MD08G1223500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015636
Physical Location & Seq
Reverse (-)
28735654 .. 28737120
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1223500.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGGTAATTGTGCTAGTGAACCAAAGACCAAGGGTGATGATGCTGCTGCAGTCCCAGTACCCGAGCCCAGGAAGGAGGAATCGGTTCAGGCCAAGGATGTGAATGCTGAGCAGCTGCAGGAAGAAAACAAGGATGAACAAAATAACGATGGTGGTCATAACAAGCAACCCTCTCTTGGGGCCTTGCTCAAGGAGGAAGAAGAGAAGACAGCTGAAGTTGCGGCAAAGCTAGAAGAAGAGAAGACAGCTGAAGTTGCGGCAAAGCTAGAAGAAGAGAAGACAGCTGAAGTTGCAACAAAGCTAAAAGAAGAGAAGACAGCTGAAGTTGCGGCAAAGCTAGAAGAAGCGGAAGAAAAGACAAAGGCCATTGAGAAGAAAGAGGAGATGGCAGTTGAGCTCGAGAAAGCACCTGAGGCAGCAGCAGCAGCAGCTGTAGTTACCCAAGAAGACAAGAAATCTGCTATTGAGGAGATCAAGAAACCCCAAGAGGTGAAGGAGACAAAAGCAGAAGAGATCAAGGAGACAAAAGCAGAAGAGACCAAGGAAACAAACCCAGAAAAGATCAAGGAGACAAATCCTGAAGTGAAGGACCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4694

Protein Analysis

200

Amino Acids

21.86

Weight (kDa)

4.65

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015636)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30481
malus_domestica MD08G1223500.v1.1 MD15G1417000.v1.1
prunus_persica Prupe.1G560900_v2.0.a1
pyrus_communis pycom15g36980
rosa_chinensis RchiOBHm_Chr7g0225161
rosa_laevigata RLG00000001842
rosa_multiflora Rmu_sc0000322.1_g000003 Rmu_sc0003737.1_g000006
rosa_roxburghii Rroxscaffold_3G00234180
rosa_rugosa Rorug07G0227700
rosa_samantha Rh7AG376000 Rh7BG362600 Rh7CG394600 Rh7DG373400
rosa_wichuraiana Rw7G031500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 221, 257, 329, 347
AcsI RAATTY 1 cut(s) 594
AcuI CTGAAG 5 cut(s) 234, 270, 306, 342, 600
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 69, 176, 177
AjnI CCWGG 1 cut(s) 68
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 4 cut(s) 491, 515, 530, 563
AlwNI CAGNNNCTG 1 cut(s) 431
Ama87I CYCGRG 2 cut(s) 62, 398
AoxI GGCC 3 cut(s) 90, 180, 363
ApeKI GCWGC 9 cut(s) 44, 47, 112, 115, 416, 419, 422, 425, 428
ApoI RAATTY 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 84
AspS9I GGNCC 2 cut(s) 180, 589
AsuHPI GGTGA 2 cut(s) 47, 502
AvaI CYCGRG 2 cut(s) 62, 398
AvaII GGWCC 1 cut(s) 589
AxyI CCTNAGG 1 cut(s) 411
BanII GRGCYC 2 cut(s) 69, 399
BbsI GAAGAC 5 cut(s) 212, 248, 284, 320, 453
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 9 cut(s) 31, 34, 102, 124, 428, 431, 434, 437, 440
BccI CCATC 2 cut(s) 143, 379
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 4 cut(s) 491, 515, 530, 563
BfaI CTAG 4 cut(s) 15, 230, 266, 338
BfmI CTRYAG 3 cut(s) 48, 116, 432
BlpI GCTNAGC 1 cut(s) 108
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 589
BmeT110I CYCGRG 2 cut(s) 62, 398
BmgT120I GGNCC 2 cut(s) 180, 589
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 70
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 31
BmuI ACTGGG 1 cut(s) 50
BpiI GAAGAC 5 cut(s) 212, 248, 284, 320, 453
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 4 cut(s) 30, 68, 93, 540
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 176, 177
Bse1I ACTGG 1 cut(s) 56
Bse21I CCTNAGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 30, 68, 93, 540
BseGI GGATG 2 cut(s) 103, 139
BseLI CCNNNNNNNGG 3 cut(s) 69, 176, 177
BseMII CTCAG 2 cut(s) 99, 402
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 2 cut(s) 395, 482
BseXI GCAGC 9 cut(s) 31, 34, 102, 124, 428, 431, 434, 437, 440
BshFI GGCC 3 cut(s) 92, 182, 365
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 2 cut(s) 62, 398
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 69, 176, 177
BsmAI GTCTC 4 cut(s) 491, 515, 530, 563
BsmFI GGGAC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 3 cut(s) 92, 182, 365
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 62, 398
Bsp1286I GDGCHC 2 cut(s) 69, 399
Bsp143I GATC 3 cut(s) 471, 513, 561
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 4 cut(s) 221, 257, 329, 347
BspANI GGCC 3 cut(s) 92, 182, 365
BspCNI CTCAG 2 cut(s) 100, 403
BspLI GGNNCC 1 cut(s) 181
BspMAI CTGCAG 2 cut(s) 52, 120
BspTNI GGTCTC 1 cut(s) 530
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 30, 68, 93, 540
BssMI GATC 3 cut(s) 471, 513, 561
BssT1I CCWWGG 3 cut(s) 30, 93, 540
Bst2UI CCWGG 1 cut(s) 70
Bst6I CTCTTC 6 cut(s) 195, 231, 267, 303, 504, 528
BstDEI CTNAG 2 cut(s) 108, 411
BstF5I GGATG 2 cut(s) 103, 139
BstKTI GATC 3 cut(s) 474, 516, 564
BstMAI GTCTC 4 cut(s) 491, 515, 530, 563
BstMBI GATC 3 cut(s) 471, 513, 561
BstMWI GCNNNNNNNGC 8 cut(s) 218, 254, 290, 326, 413, 422, 425, 428
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 3 cut(s) 48, 116, 432
BstV1I GCAGC 9 cut(s) 31, 34, 102, 124, 428, 431, 434, 437, 440
BstV2I GAAGAC 5 cut(s) 212, 248, 284, 320, 453
Bsu36I CCTNAGG 1 cut(s) 411
BsuRI GGCC 3 cut(s) 92, 182, 365
BtsCI GGATG 2 cut(s) 103, 139
CaiI CAGNNNCTG 1 cut(s) 431
Cfr13I GGNCC 2 cut(s) 180, 589
Csp6I GTAC 1 cut(s) 59
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 2 cut(s) 108, 411
DpnI GATC 3 cut(s) 473, 515, 563
DpnII GATC 3 cut(s) 471, 513, 561
Eam1104I CTCTTC 6 cut(s) 195, 231, 267, 303, 504, 528
EarI CTCTTC 6 cut(s) 195, 231, 267, 303, 504, 528
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 3 cut(s) 30, 93, 540
Eco24I GRGCYC 2 cut(s) 69, 399
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 1 cut(s) 589
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 5 cut(s) 234, 270, 306, 342, 600
Eco81I CCTNAGG 1 cut(s) 411
Eco88I CYCGRG 2 cut(s) 62, 398
EcoICRI GAGCTC 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 3 cut(s) 30, 93, 540
EcoT38I GRGCYC 2 cut(s) 69, 399
ErhI CCWWGG 3 cut(s) 30, 93, 540
FaiI YATR 1 cut(s) 159
FaqI GGGAC 1 cut(s) 38
FokI GGATG 2 cut(s) 110, 146
FriOI GRGCYC 2 cut(s) 69, 399
FspBI CTAG 4 cut(s) 15, 230, 266, 338
HaeIII GGCC 3 cut(s) 92, 182, 365
HinfI GANTC 1 cut(s) 80
HphI GGTGA 2 cut(s) 47, 502
Hpy166II GTNNAC 1 cut(s) 20
Hpy188III TCNNGA 3 cut(s) 400, 475, 578
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 3 cut(s) 67, 487, 580
HpyCH4V TGCA 3 cut(s) 50, 118, 293
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 254, 290, 326, 413, 422, 425, 428
HpyF3I CTNAG 2 cut(s) 108, 411
Kzo9I GATC 3 cut(s) 471, 513, 561
LpnPI CCDG 8 cut(s) 55, 69, 74, 82, 104, 423, 567, 591
Lsp1109I GCAGC 9 cut(s) 31, 34, 102, 124, 428, 431, 434, 437, 440
LweI GCATC 1 cut(s) 31
MaeI CTAG 4 cut(s) 15, 230, 266, 338
MaeIII GTNAC 1 cut(s) 436
MalI GATC 3 cut(s) 473, 515, 563
MboI GATC 3 cut(s) 471, 513, 561
MhlI GDGCHC 2 cut(s) 69, 399
MluCI AATT 2 cut(s) 7, 594
MnlI CCTC 7 cut(s) 70, 181, 187, 373, 406, 460, 481
MroXI GAANNNNTTC 1 cut(s) 84
MspA1I CMGCKG 6 cut(s) 115, 212, 248, 284, 320, 431
MspR9I CCNGG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 1 cut(s) 70
MwoI GCNNNNNNNGC 8 cut(s) 218, 254, 290, 326, 413, 422, 425, 428
NdeII GATC 3 cut(s) 471, 513, 561
NlaIV GGNNCC 1 cut(s) 181
PaeR7I CTCGAG 1 cut(s) 398
PctI GAATGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 2 cut(s) 180, 589
PstI CTGCAG 2 cut(s) 52, 120
PstNI CAGNNNCTG 1 cut(s) 431
PvuII CAGCTG 6 cut(s) 115, 212, 248, 284, 320, 431
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SacI GAGCTC 1 cut(s) 399
Sau3AI GATC 3 cut(s) 471, 513, 561
Sau96I GGNCC 2 cut(s) 180, 589
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 2 cut(s) 69, 399
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 3 cut(s) 48, 116, 432
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 589
SlaI CTCGAG 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 188, 398
SmoI CTYRAG 2 cut(s) 188, 398
Sse9I AATT 2 cut(s) 7, 594
SsiI CCGC 4 cut(s) 221, 257, 329, 347
SspMI CTAG 4 cut(s) 15, 230, 266, 338
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 68
StyI CCWWGG 3 cut(s) 30, 93, 540
TaqI TCGA 1 cut(s) 399
TasI AATT 2 cut(s) 7, 594
TauI GCSGC 3 cut(s) 224, 260, 332
TfiI GAWTC 1 cut(s) 80
TseI GCWGC 9 cut(s) 44, 47, 112, 115, 416, 419, 422, 425, 428
TspDTI ATGAA 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 1 cut(s) 594
XhoI CTCGAG 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 4 cut(s) 15, 230, 266, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.