MD15G1417000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015636
Physical Location & Seq
Reverse (-)
51862113 .. 51863478
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1417000.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGGTAATTGTGCTAGTGAACCAAAGACCAAGGGTGATGATGCTGCCGCAGTCCCAGCACCGGAGCCCAGGAAGGGGTCGGTTCAGGCCGAGGAGGTGAACGCTGAGCAGCTGCAGGAAGAAAACAAGGCTGAACAAAATACCGATGATGGTCATAACAAACAACCCTCTCTCGGGGCCTTGCTCAAGGAGGAAGAAGAGAAGAGAGCCGAAGTTGCGGCAAAGCTAGAAGAAGAGAAGATAGCTGAAGTTGCGGAAAGGCCAGAAGAAGAGAAGATAGCTGAAGTTGCGGCAAAGATAGAAGAAGCAGAAGAAAAGCCAAAGACCACTGAGAAGAATGAGGAGACAGCGGTTGAGCTCGAGAAATCACCTGAAGCAGCAGCACCAGCTGCAGTTACCCAAGAAGACAAGAAATGTGATATTGAGGAGAAGAGAGAGGAGATAGCGGTTGAGCTCGAGAAATCACCTGAAGTAGCAGCACCAGCTGCAGTTACCCAAGAAGACAAGAAATCTGATATTGAGGAGAAGAAAGCCGAAGAGGTAAAGGAGACAAAACCAGAAGAGACCACGACGACAAAAACAGAAGAGACCAAGGAGACAAAACCTGAAGTGAAAGACCAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4694

Protein Analysis

209

Amino Acids

22.89

Weight (kDa)

4.5

Isoelectric Point (pI)

76.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015636)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30481
malus_domestica MD08G1223500.v1.1 MD15G1417000.v1.1
prunus_persica Prupe.1G560900_v2.0.a1
pyrus_communis pycom15g36980
rosa_chinensis RchiOBHm_Chr7g0225161
rosa_laevigata RLG00000001842
rosa_multiflora Rmu_sc0000322.1_g000003 Rmu_sc0003737.1_g000006
rosa_roxburghii Rroxscaffold_3G00234180
rosa_rugosa Rorug07G0227700
rosa_samantha Rh7AG376000 Rh7BG362600 Rh7CG394600 Rh7DG373400
rosa_wichuraiana Rw7G031500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 48, 218, 254, 290, 350, 446
AcuI CTGAAG 5 cut(s) 267, 303, 393, 489, 627
AfiI CCNNNNNNNGG 5 cut(s) 61, 74, 75, 173, 174
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 8 cut(s) 112, 226, 245, 281, 358, 389, 454, 485
AluI AGCT 8 cut(s) 112, 226, 245, 281, 358, 389, 454, 485
Alw21I GWGCWC 2 cut(s) 360, 456
Alw26I GTCTC 5 cut(s) 338, 542, 557, 581, 590
Ama87I CYCGRG 3 cut(s) 173, 359, 455
AoxI GGCC 3 cut(s) 87, 177, 260
ApeKI GCWGC 8 cut(s) 44, 109, 112, 377, 380, 389, 476, 485
AspS9I GGNCC 1 cut(s) 177
AsuHPI GGTGA 4 cut(s) 47, 109, 360, 456
AvaI CYCGRG 3 cut(s) 173, 359, 455
BanII GRGCYC 3 cut(s) 69, 360, 456
BbsI GAAGAC 2 cut(s) 411, 507
Bbv12I GWGCWC 2 cut(s) 360, 456
BbvI GCAGC 8 cut(s) 31, 99, 121, 376, 389, 392, 472, 488
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 5 cut(s) 338, 542, 557, 581, 590
BfaI CTAG 2 cut(s) 15, 227
BfmI CTRYAG 3 cut(s) 113, 390, 486
BlpI GCTNAGC 1 cut(s) 105
Bme1390I CCNGG 1 cut(s) 70
BmeT110I CYCGRG 3 cut(s) 173, 359, 455
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 2 cut(s) 66, 178
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 2 cut(s) 411, 507
Bpu1102I GCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 170
BsaI GGTCTC 2 cut(s) 557, 581
BsaJI CCNNGG 4 cut(s) 30, 68, 90, 591
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 74, 75, 173, 174
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 30, 68, 90, 591
BseLI CCNNNNNNNGG 5 cut(s) 61, 74, 75, 173, 174
BseMII CTCAG 2 cut(s) 96, 321
BseRI GAGGAG 5 cut(s) 107, 356, 440, 452, 536
BseXI GCAGC 8 cut(s) 31, 99, 121, 376, 389, 392, 472, 488
BseYI CCCAGC 1 cut(s) 55
BshFI GGCC 3 cut(s) 89, 179, 262
BsiHKAI GWGCWC 2 cut(s) 360, 456
BsiHKCI CYCGRG 3 cut(s) 173, 359, 455
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 5 cut(s) 61, 74, 75, 173, 174
BsmAI GTCTC 5 cut(s) 338, 542, 557, 581, 590
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 3 cut(s) 89, 179, 262
Bso31I GGTCTC 2 cut(s) 557, 581
BsoBI CYCGRG 3 cut(s) 173, 359, 455
Bsp1286I GDGCHC 3 cut(s) 69, 360, 456
Bsp1720I GCTNAGC 1 cut(s) 105
BspACI CCGC 6 cut(s) 48, 218, 254, 290, 350, 446
BspANI GGCC 3 cut(s) 89, 179, 262
BspCNI CTCAG 2 cut(s) 97, 322
BspLI GGNNCC 2 cut(s) 66, 178
BspMAI CTGCAG 3 cut(s) 117, 394, 490
BspTNI GGTCTC 2 cut(s) 557, 581
BssECI CCNNGG 4 cut(s) 30, 68, 90, 591
BssT1I CCWWGG 2 cut(s) 30, 591
Bst2UI CCWGG 1 cut(s) 70
Bst6I CTCTTC 8 cut(s) 192, 197, 228, 264, 425, 531, 555, 579
BstAPI GCANNNNNTGC 2 cut(s) 389, 485
BstDEI CTNAG 2 cut(s) 105, 330
BstMAI GTCTC 5 cut(s) 338, 542, 557, 581, 590
BstMWI GCNNNNNNNGC 8 cut(s) 56, 215, 251, 287, 386, 389, 482, 485
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 3 cut(s) 113, 390, 486
BstV1I GCAGC 8 cut(s) 31, 99, 121, 376, 389, 392, 472, 488
BstV2I GAAGAC 2 cut(s) 411, 507
BsuRI GGCC 3 cut(s) 89, 179, 262
BtsIMutI CAGTG 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 177
DdeI CTNAG 2 cut(s) 105, 330
Eam1104I CTCTTC 8 cut(s) 192, 197, 228, 264, 425, 531, 555, 579
EarI CTCTTC 8 cut(s) 192, 197, 228, 264, 425, 531, 555, 579
Ecl136II GAGCTC 2 cut(s) 358, 454
Eco130I CCWWGG 2 cut(s) 30, 591
Eco24I GRGCYC 3 cut(s) 69, 360, 456
Eco31I GGTCTC 2 cut(s) 557, 581
Eco53kI GAGCTC 2 cut(s) 358, 454
Eco57I CTGAAG 5 cut(s) 267, 303, 393, 489, 627
Eco88I CYCGRG 3 cut(s) 173, 359, 455
EcoICRI GAGCTC 2 cut(s) 358, 454
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 30, 591
EcoT38I GRGCYC 3 cut(s) 69, 360, 456
ErhI CCWWGG 2 cut(s) 30, 591
FaiI YATR 2 cut(s) 156, 625
FaqI GGGAC 1 cut(s) 38
FriOI GRGCYC 3 cut(s) 69, 360, 456
FspBI CTAG 2 cut(s) 15, 227
GsaI CCCAGC 1 cut(s) 59
HaeIII GGCC 3 cut(s) 89, 179, 262
HapII CCGG 1 cut(s) 62
HpaII CCGG 1 cut(s) 62
HphI GGTGA 4 cut(s) 47, 109, 360, 456
Hpy166II GTNNAC 2 cut(s) 20, 100
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 2 cut(s) 361, 457
Hpy8I GTNNAC 2 cut(s) 20, 100
Hpy99I CGWCG 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 115, 392, 488
HpyF10VI GCNNNNNNNGC 8 cut(s) 56, 215, 251, 287, 386, 389, 482, 485
HpyF3I CTNAG 2 cut(s) 105, 330
LmnI GCTCC 1 cut(s) 64
Lsp1109I GCAGC 8 cut(s) 31, 99, 121, 376, 389, 392, 472, 488
LweI GCATC 1 cut(s) 31
MaeI CTAG 2 cut(s) 15, 227
MaeIII GTNAC 2 cut(s) 394, 490
MhlI GDGCHC 3 cut(s) 69, 360, 456
MluCI AATT 1 cut(s) 7
MnlI CCTC 9 cut(s) 85, 88, 178, 184, 334, 418, 430, 514, 532
MspA1I CMGCKG 4 cut(s) 112, 350, 389, 485
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 70
MvaI CCWGG 1 cut(s) 70
MwoI GCNNNNNNNGC 8 cut(s) 56, 215, 251, 287, 386, 389, 482, 485
NlaIV GGNNCC 2 cut(s) 66, 178
NmeAIII GCCGAG 1 cut(s) 115
PaeR7I CTCGAG 2 cut(s) 359, 455
Psp124BI GAGCTC 2 cut(s) 360, 456
Psp6I CCWGG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 66, 178
PspPI GGNCC 1 cut(s) 177
PstI CTGCAG 3 cut(s) 117, 394, 490
PvuII CAGCTG 3 cut(s) 112, 389, 485
SacI GAGCTC 2 cut(s) 360, 456
Sau96I GGNCC 1 cut(s) 177
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 3 cut(s) 69, 360, 456
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 3 cut(s) 113, 390, 486
Sfr274I CTCGAG 2 cut(s) 359, 455
SlaI CTCGAG 2 cut(s) 359, 455
SmlI CTYRAG 3 cut(s) 185, 359, 455
SmoI CTYRAG 3 cut(s) 185, 359, 455
Sse9I AATT 1 cut(s) 7
SsiI CCGC 6 cut(s) 48, 218, 254, 290, 350, 446
SspMI CTAG 2 cut(s) 15, 227
SstI GAGCTC 2 cut(s) 360, 456
StyD4I CCNGG 1 cut(s) 68
StyI CCWWGG 2 cut(s) 30, 591
TaqI TCGA 2 cut(s) 360, 456
TasI AATT 1 cut(s) 7
TauI GCSGC 3 cut(s) 50, 221, 293
TscAI CASTG 1 cut(s) 334
TseI GCWGC 8 cut(s) 44, 109, 112, 377, 380, 389, 476, 485
TspRI CASTG 1 cut(s) 334
XhoI CTCGAG 2 cut(s) 359, 455
XspI CTAG 2 cut(s) 15, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.