MD08G1229900.v1.1

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002499
Physical Location & Seq
Reverse (-)
29563428 .. 29566802
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1229900.v1.1.491

Sequence Viewer

Length: 543 bp
ATGAAGAAATGGGAAAGAAACATAATGAAAATGACATTGAATGGAACAATGTTGTCTTTCCCTCCAGCCATGCTTTTAATACCAACCGAATTCCCTTATCTCCTCGCATTTTACCTTTTGATTCCTCCTAAAGGTCTTTTACTGGTAACCCATGAACCTGGCTCTGTCAGTACTGCACCAGGTCCTCACATCAGAGGAAGTCCGTTGAAAGCTTATTCTCTCGGTGACAAAGAAGTCAATCTCTTGCTCCCTGGTACAGGAAGGTTAAGGGTGCAACTAGGTGTAGACATGCCGGCCCGGGCCAATCGTAAGATTGTGCGGGAAGAGGTCAATTCAACAGAAGTAGAACTATTTGAATTCTACGACGTGGTTCTTTATATGGGGATAATCGACATATTGCAGGAGTACAATGAGAACAAGAAACTCGAGCACGCTTACAAATCGTCGAAGTTTGACCCTCAACGCGTTTCTGCTGTCGAACCGGAGCTGTACGCTAAGCGTTTCATCAATTTCTTGGAGAAAGTTTTCCCGGTCCTGCCGTAA

Protein Analysis

181

Amino Acids

20.54

Weight (kDa)

8.71

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 100 - 174 3.5e-20 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 285
AccII CGCG 1 cut(s) 465
AciI CCGC 1 cut(s) 319
AcsI RAATTY 2 cut(s) 89, 356
AfaI GTAC 4 cut(s) 172, 256, 407, 491
AfiI CCNNNNNNNGG 2 cut(s) 131, 257
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 4 cut(s) 40, 208, 336, 356
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 3 cut(s) 157, 178, 250
AluBI AGCT 2 cut(s) 212, 487
AluI AGCT 2 cut(s) 212, 487
Alw21I GWGCWC 1 cut(s) 432
Ama87I CYCGRG 2 cut(s) 297, 425
AoxI GGCC 2 cut(s) 294, 300
ApoI RAATTY 2 cut(s) 89, 356
Asp700I GAANNNNTTC 1 cut(s) 524
AspS9I GGNCC 4 cut(s) 182, 295, 300, 532
AsuC2I CCSGG 3 cut(s) 298, 299, 530
AsuHPI GGTGA 1 cut(s) 236
AvaI CYCGRG 2 cut(s) 297, 425
AvaII GGWCC 2 cut(s) 182, 532
Bbv12I GWGCWC 1 cut(s) 432
BceAI ACGGC 1 cut(s) 523
BciT130I CCWGG 3 cut(s) 159, 180, 252
BcnI CCSGG 3 cut(s) 298, 299, 530
BfaI CTAG 1 cut(s) 278
BlpI GCTNAGC 1 cut(s) 495
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 6 cut(s) 159, 180, 252, 298, 299, 530
Bme18I GGWCC 2 cut(s) 182, 532
BmeT110I CYCGRG 2 cut(s) 297, 425
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 4 cut(s) 182, 295, 300, 532
BmrFI CCNGG 6 cut(s) 159, 180, 252, 298, 299, 530
BpmI CTGGAG 1 cut(s) 48
Bpu1102I GCTNAGC 1 cut(s) 495
BpuMI CCSGG 3 cut(s) 298, 299, 530
BsaJI CCNNGG 2 cut(s) 250, 297
BsaWI WCCGGW 1 cut(s) 481
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 257
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 147
BseBI CCWGG 3 cut(s) 159, 180, 252
BseDI CCNNGG 2 cut(s) 250, 297
BseLI CCNNNNNNNGG 2 cut(s) 131, 257
BseNI ACTGG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 92
BsgI GTGCAG 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 2 cut(s) 296, 302
BsiHKAI GWGCWC 1 cut(s) 432
BsiHKCI CYCGRG 2 cut(s) 297, 425
BsiSI CCGG 4 cut(s) 293, 298, 482, 530
BslI CCNNNNNNNGG 2 cut(s) 131, 257
BsnI GGCC 2 cut(s) 296, 302
BsoBI CYCGRG 2 cut(s) 297, 425
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 1 cut(s) 319
BspANI GGCC 2 cut(s) 296, 302
BspFNI CGCG 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 1 cut(s) 147
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 2 cut(s) 250, 297
Bst2UI CCWGG 3 cut(s) 159, 180, 252
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 294, 432
BstDEI CTNAG 1 cut(s) 495
BstEII GGTNACC 1 cut(s) 145
BstENI CCTNNNNNAGG 2 cut(s) 129, 255
BstFNI CGCG 1 cut(s) 465
BstNI CCWGG 3 cut(s) 159, 180, 252
BstNSI RCATGY 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 6 cut(s) 157, 178, 250, 296, 297, 528
BstUI CGCG 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 158
BsuRI GGCC 2 cut(s) 296, 302
BtrI CACGTC 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 294, 432
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 4 cut(s) 182, 295, 300, 532
Cfr9I CCCGGG 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 178
Csp6I GTAC 4 cut(s) 171, 255, 406, 490
CviAII CATG 3 cut(s) 70, 152, 289
CviJI RGCY 6 cut(s) 68, 162, 212, 296, 302, 487
CviKI_1 RGCY 6 cut(s) 68, 162, 212, 296, 302, 487
CviQI GTAC 4 cut(s) 171, 255, 406, 490
DdeI CTNAG 1 cut(s) 495
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 182, 532
Eco88I CYCGRG 2 cut(s) 297, 425
Eco91I GGTNACC 1 cut(s) 145
EcoNI CCTNNNNNAGG 2 cut(s) 129, 255
EcoO109I RGGNCCY 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 145
EcoRI GAATTC 2 cut(s) 89, 356
EcoRII CCWGG 3 cut(s) 157, 178, 250
FaeI CATG 3 cut(s) 73, 155, 292
FaiI YATR 7 cut(s) 23, 71, 153, 290, 378, 380, 395
FatI CATG 3 cut(s) 69, 151, 288
FauI CCCGC 1 cut(s) 312
FblI GTMKAC 1 cut(s) 285
FspBI CTAG 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 2 cut(s) 296, 302
HapII CCGG 4 cut(s) 293, 298, 482, 530
Hin1II CATG 3 cut(s) 73, 155, 292
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 121
HpaII CCGG 4 cut(s) 293, 298, 482, 530
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 286
Hpy99I CGWCG 2 cut(s) 368, 448
HpyAV CCTTC 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 3 cut(s) 176, 274, 400
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 73, 155, 292
KroI GCCGGC 1 cut(s) 292
KroNI GCCGGC 1 cut(s) 294
LmnI GCTCC 2 cut(s) 252, 484
MabI ACCWGGT 1 cut(s) 178
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 145, 224
MboII GAAGA 2 cut(s) 16, 335
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 4 cut(s) 89, 331, 356, 508
MluI ACGCGT 1 cut(s) 463
MnlI CCTC 7 cut(s) 72, 113, 135, 188, 195, 319, 468
MroNI GCCGGC 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 2 cut(s) 77, 266
MspI CCGG 4 cut(s) 293, 298, 482, 530
MspR9I CCNGG 6 cut(s) 159, 180, 252, 298, 299, 530
MvaI CCWGG 3 cut(s) 159, 180, 252
MvnI CGCG 1 cut(s) 465
NaeI GCCGGC 1 cut(s) 294
NciI CCSGG 3 cut(s) 298, 299, 530
NgoMIV GCCGGC 1 cut(s) 292
NlaIII CATG 3 cut(s) 73, 155, 292
NmuCI GTSAC 1 cut(s) 224
NspI RCATGY 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 425
PdiI GCCGGC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 3 cut(s) 157, 178, 250
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 3 cut(s) 157, 178, 250
PspPI GGNCC 4 cut(s) 182, 295, 300, 532
PspPPI RGGWCCY 1 cut(s) 182
PspXI VCTCGAGB 1 cut(s) 425
RsaI GTAC 4 cut(s) 172, 256, 407, 491
RsaNI GTAC 4 cut(s) 171, 255, 406, 490
SaqAI TTAA 2 cut(s) 77, 266
Sau96I GGNCC 4 cut(s) 182, 295, 300, 532
ScaI AGTACT 1 cut(s) 172
ScrFI CCNGG 6 cut(s) 159, 180, 252, 298, 299, 530
SduI GDGCHC 1 cut(s) 432
SexAI ACCWGGT 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 425
SinI GGWCC 2 cut(s) 182, 532
SlaI CTCGAG 1 cut(s) 425
SmaI CCCGGG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
SrfI GCCCGGGC 1 cut(s) 299
Sse9I AATT 4 cut(s) 89, 331, 356, 508
SsiI CCGC 1 cut(s) 319
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 6 cut(s) 157, 178, 250, 296, 297, 528
TaiI ACGT 1 cut(s) 369
TaqI TCGA 4 cut(s) 390, 426, 446, 477
TasI AATT 4 cut(s) 89, 331, 356, 508
TatI WGTACW 2 cut(s) 170, 405
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 2 cut(s) 77, 266
Tru9I TTAA 2 cut(s) 77, 266
TseFI GTSAC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 4 cut(s) 17, 41, 168, 493
TspGWI ACGGA 1 cut(s) 192
TspMI CCCGGG 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 182, 532
XagI CCTNNNNNAGG 2 cut(s) 129, 255
XapI RAATTY 2 cut(s) 89, 356
XceI RCATGY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 425
XmaI CCCGGG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 278
ZrmI AGTACT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.