pycom08g20000

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002499
Physical Location & Seq
Reverse (-)
19914922 .. 19917450
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20000.1

Sequence Viewer

Length: 786 bp
ATGGTCATGGGGAATATGTTTTGCACTGAATTACAAATTCATCGCCGTTATAATTTGAATGGTTCGACTATTGGAAGATTTACAGACAAAGATAAAATTGATGAGAATAACACATTGAAAGATCTCGATCTGACATTTGAATTTCATGTGGACAAACTGTTGCGAGAAGCACATTTTAAACAAATATCACTAGACTGCATGTTTCTACAATCTCAGCAAATTATCGATTATAGCCTTCTGTTGGGTCTACGTTTTAGAGCTCCCGAGGATCTGAAGGGATTTTCACAACCTCCTGCCACAACGCACAACTATGAGAGTTTACTTGCTAATGATGGTGTGACTTTGCAAGGGGAGCTTTTGATTCCTCCTAAAGGTCTTCTACTAGTAACCCATGAACCTGGCTCTGTCAGTACTGCACCAGGTCCTCACATCAGAGGAAGTCGGTTGAAAGCTTATTCTCTCGGTGACAAAGAAGTCGATCTCTTACTCCCTGGTACAGGAAGGTTAAGGGTGCAATTAGGCGTAAACATGCCGGCCCTGGCCAAACGTAAGCATGCACAGGAAGAGGTCGATTCAACAGAAGTAGAACTCTTTGAATTCTACCACGTGGTTCTTTATATGGGGATAATCGACATATTGCAGGAGTACAATGTGGAAAAGAAATTCGAGCATGCTTACAAATCGTTGAAGAATATTGACCCTCAACGCATTTCTGCTGTCGAACCGGAGCTGTACGCTAAGCGTTTCATCAATTTCATGAAGAGTGTTTTCCCGGACCTGCCGTAA

Protein Analysis

262

Amino Acids

29.78

Weight (kDa)

6.0

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 1 - 255 5.5e-51 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 51
AasI GACNNNNNNGTC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 247
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 4 cut(s) 36, 140, 596, 662
AcuI CTGAAG 1 cut(s) 293
AcvI CACGTG 1 cut(s) 607
AfaI GTAC 4 cut(s) 412, 496, 647, 734
AfiI CCNNNNNNNGG 3 cut(s) 241, 371, 497
AgsI TTSAA 7 cut(s) 58, 118, 140, 448, 576, 596, 688
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 4 cut(s) 397, 418, 490, 537
AluBI AGCT 4 cut(s) 260, 355, 452, 730
AluI AGCT 4 cut(s) 260, 355, 452, 730
Alw21I GWGCWC 1 cut(s) 262
AlwI GGATC 1 cut(s) 276
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 2 cut(s) 534, 540
ApoI RAATTY 4 cut(s) 36, 140, 596, 662
AspS9I GGNCC 3 cut(s) 422, 535, 775
AsuC2I CCSGG 1 cut(s) 773
AsuHPI GGTGA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 422, 775
BalI TGGCCA 1 cut(s) 542
BanII GRGCYC 1 cut(s) 262
BbrPI CACGTG 1 cut(s) 607
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 262
BccI CCATC 1 cut(s) 326
BceAI ACGGC 2 cut(s) 30, 766
BciT130I CCWGG 4 cut(s) 399, 420, 492, 539
BcnI CCSGG 1 cut(s) 773
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 2 cut(s) 191, 383
BglII AGATCT 1 cut(s) 121
BlpI GCTNAGC 1 cut(s) 738
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 5 cut(s) 399, 420, 492, 539, 773
Bme18I GGWCC 2 cut(s) 422, 775
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 3 cut(s) 422, 535, 775
BmrFI CCNGG 5 cut(s) 399, 420, 492, 539, 773
BpiI GAAGAC 1 cut(s) 368
Bpu1102I GCTNAGC 1 cut(s) 738
BpuMI CCSGG 1 cut(s) 773
Bsa29I ATCGAT 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 607
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 264, 490, 537
BsaWI WCCGGW 1 cut(s) 724
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 371, 497
Bse118I RCCGGY 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 4 cut(s) 399, 420, 492, 539
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 264, 490, 537
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 241, 371, 497
BseMII CTCAG 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 399
BshFI GGCC 2 cut(s) 536, 542
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 3 cut(s) 533, 725, 773
BslI CCNNNNNNNGG 3 cut(s) 241, 371, 497
BsnI GGCC 2 cut(s) 536, 542
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 262
Bsp143I GATC 4 cut(s) 121, 127, 268, 478
Bsp1720I GCTNAGC 1 cut(s) 738
BspANI GGCC 2 cut(s) 536, 542
BspCNI CTCAG 1 cut(s) 226
BspDI ATCGAT 1 cut(s) 225
BspHI TCATGA 1 cut(s) 756
BspPI GGATC 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 532
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 264, 490, 537
BssMI GATC 4 cut(s) 121, 127, 268, 478
Bst2UI CCWGG 4 cut(s) 399, 420, 492, 539
Bst4CI ACNGT 1 cut(s) 159
Bst6I CTCTTC 2 cut(s) 558, 755
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 534, 555, 672
BstDEI CTNAG 2 cut(s) 213, 738
BstENI CCTNNNNNAGG 2 cut(s) 369, 495
BstKTI GATC 4 cut(s) 124, 130, 271, 481
BstMBI GATC 4 cut(s) 121, 127, 268, 478
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNI CCWGG 4 cut(s) 399, 420, 492, 539
BstNSI RCATGY 4 cut(s) 202, 532, 557, 674
BstSCI CCNGG 5 cut(s) 397, 418, 490, 537, 771
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 121, 268
BstXI CCANNNNNNTGG 1 cut(s) 398
BstYI RGATCY 2 cut(s) 121, 268
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 2 cut(s) 536, 542
BsuTUI ATCGAT 1 cut(s) 225
BtgZI GCGATG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 534, 555, 672
CciI TCATGA 1 cut(s) 756
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 3 cut(s) 422, 535, 775
ClaI ATCGAT 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 418
Csp6I GTAC 4 cut(s) 411, 495, 646, 733
CviAII CATG 8 cut(s) 7, 146, 199, 392, 529, 554, 671, 757
CviJI RGCY 8 cut(s) 234, 260, 355, 402, 452, 536, 542, 730
CviKI_1 RGCY 8 cut(s) 234, 260, 355, 402, 452, 536, 542, 730
CviQI GTAC 4 cut(s) 411, 495, 646, 733
DdeI CTNAG 2 cut(s) 213, 738
DpnI GATC 4 cut(s) 123, 129, 270, 480
DpnII GATC 4 cut(s) 121, 127, 268, 478
DraI TTTAAA 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 2 cut(s) 558, 755
EarI CTCTTC 2 cut(s) 558, 755
Ecl136II GAGCTC 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 2 cut(s) 422, 775
Eco53kI GAGCTC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 293
Eco72I CACGTG 1 cut(s) 607
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 260
EcoNI CCTNNNNNAGG 2 cut(s) 369, 495
EcoO109I RGGNCCY 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 4 cut(s) 397, 418, 490, 537
EcoT38I GRGCYC 1 cut(s) 262
FaeI CATG 8 cut(s) 10, 149, 202, 395, 532, 557, 674, 760
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FatI CATG 8 cut(s) 6, 145, 198, 391, 528, 553, 670, 756
FblI GTMKAC 1 cut(s) 247
FriOI GRGCYC 1 cut(s) 262
FspBI CTAG 2 cut(s) 191, 383
HaeIII GGCC 2 cut(s) 536, 542
HapII CCGG 3 cut(s) 533, 725, 773
Hin1II CATG 8 cut(s) 10, 149, 202, 395, 532, 557, 674, 760
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 2 cut(s) 361, 572
HpaII CCGG 3 cut(s) 533, 725, 773
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 4 cut(s) 151, 248, 320, 526
Hpy188I TCNGA 3 cut(s) 132, 273, 434
Hpy188III TCNNGA 3 cut(s) 125, 263, 757
Hpy8I GTNNAC 4 cut(s) 151, 248, 320, 526
HpyAV CCTTC 3 cut(s) 245, 268, 495
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4IV ACGT 3 cut(s) 250, 547, 606
HpyCH4V TGCA 7 cut(s) 24, 198, 346, 416, 514, 557, 640
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 2 cut(s) 213, 738
HpySE526I ACGT 3 cut(s) 250, 547, 606
Hsp92II CATG 8 cut(s) 10, 149, 202, 395, 532, 557, 674, 760
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Kzo9I GATC 4 cut(s) 121, 127, 268, 478
LmnI GCTCC 3 cut(s) 265, 352, 727
MabI ACCWGGT 1 cut(s) 418
MaeI CTAG 2 cut(s) 191, 383
MaeII ACGT 3 cut(s) 250, 547, 606
MaeIII GTNAC 3 cut(s) 337, 385, 464
MalI GATC 4 cut(s) 123, 129, 270, 480
MboI GATC 4 cut(s) 121, 127, 268, 478
MboII GAAGA 5 cut(s) 87, 368, 575, 700, 772
MflI RGATCY 2 cut(s) 121, 268
MhlI GDGCHC 1 cut(s) 262
MlsI TGGCCA 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 542
MnlI CCTC 7 cut(s) 259, 300, 375, 428, 435, 559, 711
Mox20I TGGCCA 1 cut(s) 542
MroNI GCCGGC 1 cut(s) 532
MscI TGGCCA 1 cut(s) 542
MseI TTAA 2 cut(s) 177, 506
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 542
MspI CCGG 3 cut(s) 533, 725, 773
MspR9I CCNGG 5 cut(s) 399, 420, 492, 539, 773
MvaI CCWGG 4 cut(s) 399, 420, 492, 539
MwoI GCNNNNNNNGC 1 cut(s) 352
NaeI GCCGGC 1 cut(s) 534
NciI CCSGG 1 cut(s) 773
NdeII GATC 4 cut(s) 121, 127, 268, 478
NgoMIV GCCGGC 1 cut(s) 532
NlaIII CATG 8 cut(s) 10, 149, 202, 395, 532, 557, 674, 760
NmuCI GTSAC 2 cut(s) 337, 464
NspI RCATGY 4 cut(s) 202, 532, 557, 674
PaeI GCATGC 2 cut(s) 557, 674
PagI TCATGA 1 cut(s) 756
PdiI GCCGGC 1 cut(s) 534
PfeI GAWTC 2 cut(s) 361, 572
PfoI TCCNGGA 1 cut(s) 771
PmaCI CACGTG 1 cut(s) 607
PmlI CACGTG 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 607
PpuMI RGGWCCY 1 cut(s) 422
PsiI TTATAA 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 262
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 4 cut(s) 397, 418, 490, 537
PspCI CACGTG 1 cut(s) 607
PspGI CCWGG 4 cut(s) 397, 418, 490, 537
PspPI GGNCC 3 cut(s) 422, 535, 775
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 2 cut(s) 121, 268
RsaI GTAC 4 cut(s) 412, 496, 647, 734
RsaNI GTAC 4 cut(s) 411, 495, 646, 733
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 177, 506
Sau3AI GATC 4 cut(s) 121, 127, 268, 478
Sau96I GGNCC 3 cut(s) 422, 535, 775
ScaI AGTACT 1 cut(s) 412
ScrFI CCNGG 5 cut(s) 399, 420, 492, 539, 773
SduI GDGCHC 1 cut(s) 262
SexAI ACCWGGT 1 cut(s) 418
SinI GGWCC 2 cut(s) 422, 775
SmiMI CAYNNNNRTG 1 cut(s) 309
SpeI ACTAGT 1 cut(s) 382
SphI GCATGC 2 cut(s) 557, 674
SspI AATATT 1 cut(s) 694
SspMI CTAG 2 cut(s) 191, 383
SstI GAGCTC 1 cut(s) 262
StyD4I CCNGG 5 cut(s) 397, 418, 490, 537, 771
TaaI ACNGT 1 cut(s) 159
TaiI ACGT 3 cut(s) 253, 550, 609
TaqI TCGA 8 cut(s) 65, 126, 225, 477, 570, 630, 666, 720
TatI WGTACW 2 cut(s) 410, 645
TfiI GAWTC 2 cut(s) 361, 572
Tru1I TTAA 2 cut(s) 177, 506
Tru9I TTAA 2 cut(s) 177, 506
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 2 cut(s) 337, 464
Tsp45I GTSAC 2 cut(s) 337, 464
TspDTI ATGAA 6 cut(s) 29, 134, 408, 736, 745, 773
TspRI CASTG 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 422, 775
XagI CCTNNNNNAGG 2 cut(s) 369, 495
XapI RAATTY 4 cut(s) 36, 140, 596, 662
XceI RCATGY 4 cut(s) 202, 532, 557, 674
XmiI GTMKAC 1 cut(s) 247
XspI CTAG 2 cut(s) 191, 383
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.