Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009033

Physical Location & Seq

Genomic Coordinates Reverse (-)

205385 .. 216338

Gene Structure 10954 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1001500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGGAGCCGGACTCTGCAATTTCGACGCCCTCCCGGTATCCCATTCGTCCTCCTCGTCAGCTTTCATGGGTGAATCTCCCCAGAAATCTCATACTTGCATATCAAAGCCTTGGCGTGGTTTATGGGGACCTCAGTACTTCTCCTCTCTACGTTTATACTAGCACATTCACAGGGAAGCTGCACAACCATAACTCTGAGGAAGTGATATTTGGTGCATTTTCCTTAATCTTCTGGACCATTACAATGGTACCCTTGCTCAAGTACGTCTTTATCCTATTGAGTGCCGACGATAATGGTGAAGGTGGGACTTTTGCGCTTTACTCGCTTCTATGCCGCCATGCAAAGTTTAGTTTACTTCCCAACCAGCAAGCAGCTGATGAGGAGCTCTCAGCTTACAAATACGGTCCTTCATCACAGGTTGTAAGCTCGTCCCGTTTAAAGATATTTTTGGAGAAGCATAAAAGGTTACGGACGGCCCTACTAGTGGTGGTATTGTTAGGCGCTTGCATGGTCATAGGAGATGGTGTACTCACTCCAGCAATTTCAGTTTTATCATCGGTATCAGGGTTACAAGTTACAGAGGAGAAGTTAACTGATGGGGAACTCCTCTTGCTTGCCTGTGTCATATTGGTTGGCCTGTTTGCTCTGCAGCATTGTGGCACCCACAGGGTAGCATTTTTGTTTGCACCTATTGTAATAATCTGGTTGATATCAATATTTTCCGTTGGCCTGTACAATACAATACATTGGAACCCGGCTGTTGTTCGTGCATTATCGCCCCATTATATTATCAAGTTTTTCCGTGAGACTGGTAAAGATGGTTGGATTTCTCTTGGTGGGGTTCTTCTCTCGATAACTGGTACTGAAGCTATGTTTGCAGATCTTGGTCATTTCACTGCCTTATCGATAAGACTTGCGTTTGCATTCGCTATATATCCTTGTTTGGTGGTACAATACATGGGTCAGGCTGCTTTCCTGTCAAAAAATCCTGACCACAATCTTTACAGTTTCTATGATTCAATACCTGCTCCTGTATTTTGGCCTGTCTTTGTTGTTGCCACCCTCGCATCAATTGTTGGAAGCCAGGCTGTTATAACTGCTACTTTCTCAATCATCAAGCAATGTCATGCCCTTGGTTGTTTCCCCCGAGTAAAAGTTGTCCACACTTCAAAGCATATATATGGGCAGATCTACATTCCAGAAATTAATTGGATACTTATGATCCTTACTCTTGCCATAACTATTGGATTTCAAGACACTACTTTAATTGGAAATGCTTACGGACTTGCTTGCATGATGGTTATGTTTATCACGACATTCCTTATGGCACTGGTAATAATCTTTGTTTGGCAGAAAAGTGTGGTTCTGGCTGCGATTTTTCTCATCTTTTTCTGGTTCATTGAGGGAATGTACTTATCAGCAGCACTCATTAAAGTACCTCAGGGAGGATGGGTCCCTTTTGTTCTCTCATTCATCTTTATGATTGTTATGTACGTCTGGCATTATGGTACGCGGAAGAAGTATAACTATGATCTGCACAACAAAGTCTCGTTAAAATGGCTACTTGGTCTTGGCCCAAGCCTTGGTATAGTCCGGGTGCCTGGAATAGGCCTCATATACTCAGAGTTGGCAACAGGAGTTCCTGCAATATTCTCTCACTTTGTAACAAATCTTCCTGCATTCCACAAGGTGTTGGTTTTTGTTTGTGTAAAATCAGTTCAGGTGCCTTGTGTTTCACCTGAAGAACGATTTCTGATTGGCCGGATATGTCCAAGATCGTATCGTATGTATAGGTGCATTGTCAGGTATGGGTACAAGGACATTCAACGGGATGATGGGGACTTTGAGGACCTGCTCATACAGAGCATAGCAGAGTTCATCCAGATGGAAGCAGTGGAACCACAATTCTCAAGCCCAGAGATATCATCATTTGATGGAAGGATGGCGGTTATAAGCACTAGGACAAGTTCGAGCTTGATAAGCAGTGAACAAGAGGGTTTTGGCGTGGGGGATTCCATCCAGAGCAGCAAATCTCTGACCCTGCAGAGTATGGTATCAGTGTATGATGAGGAGAACCCACAAATGAGGAGGTGCCAAGTAAGGTTTCAGGTACCATCAAACGTGGGTATGGATCCTGCAGTAAGGGAAGAGCTGATGGATTTGATCCAGGCCAAGGAAGCAGGGGTTGCGTATATAATGGGGCACTCGTATGTGAAGGCGAGGAGGTCGTCTTCATTTCTGAAGAAGCTTGTGATTGATATGGGGTATTCTTTTCTTAGGAAGAACTGCAGGGGCCCTGCTGTGGCGCTTAACATTCCTCACATCAGTCTTATCGAAGTTGGGATGATATACTATGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0016043 GO:0016049 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0032502 GO:0032989 GO:0034220 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048589 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0071804 GO:0071805 GO:0071840 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

787

Amino Acids

87.64

Weight (kDa)

8.57

Isoelectric Point (pI)

44.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	32 - 521	5.7e-164	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	534 - 784	4e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02050
fragaria_vesca	FvH4_6g54410 FvH4_6g54410
malus_domestica	MD09G1001500.v1.1 MD17G1005100.v1.1
prunus_persica	Prupe.3G314700_v2.0.a1 Prupe.3G314700_v2.0.a1
pyrus_communis	pycom17g00130
rosa_chinensis	RchiOBHm_Chr2g0176661
rosa_laevigata	RLG00000022433
rosa_multiflora	Rmu_co8314729.1_g000001 Rmu_co8416913.1_g000001 Rmu_sc0001417.1_g000018 Rmu_sc0012494.1_g000001 Rmu_ssc0000468.1_g000012
rosa_rugosa	Rorug02G0597400
rosa_samantha	Rh2AG674700 Rh2BG685800 Rh2CG649100 Rh2DG700000
rosa_wichuraiana	Rw0G015660 Rw2G055370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1094, 1952
Acc36I	ACCTGC	2 cut(s)	1033, 1860
Acc65I	GGTACC	2 cut(s)	247, 2110
AccB1I	GGYRCC	6 cut(s)	247, 659, 1597, 1723, 2091, 2110
AccB7I	CCANNNNNTGG	1 cut(s)	1583
AccII	CGCG	1 cut(s)	1513
AciI	CCGC	3 cut(s)	334, 1513, 1946
AclWI	GGATC	4 cut(s)	1216, 2126, 2139, 2158
AcoI	YGGCCR	1 cut(s)	1759
AcuI	CTGAAG	3 cut(s)	885, 1761, 2261
AcyI	GRCGYC	1 cut(s)	26
AdeI	CACNNNGTG	1 cut(s)	1690
AfiI	CCNNNNNNNGG	7 cut(s)	115, 484, 1445, 1583, 1607, 2173, 2302
AgsI	TTSAA	4 cut(s)	1020, 1170, 1253, 1826
AhlI	ACTAGT	1 cut(s)	481
AjnI	CCWGG	3 cut(s)	1083, 1600, 2166
AjuI	GAANNNNNNNTTGG	4 cut(s)	192, 224, 2088, 2120
Alw21I	GWGCWC	1 cut(s)	387
Alw26I	GTCTC	2 cut(s)	800, 1552
AlwI	GGATC	4 cut(s)	1216, 2126, 2139, 2158
Ama87I	CYCGRG	1 cut(s)	1146
AoxI	GGCC	9 cut(s)	474, 634, 727, 1040, 1573, 1609, 1759, 2169, 2293
ApaI	GGGCCC	1 cut(s)	2297
ApeKI	GCWGC	7 cut(s)	178, 371, 649, 968, 1370, 1421, 2025
AseI	ATTAAT	1 cut(s)	1206
Asp700I	GAANNNNTTC	1 cut(s)	1749
Asp718I	GGTACC	2 cut(s)	247, 2110
AspLEI	GCGC	3 cut(s)	316, 503, 2308
AspS9I	GGNCC	9 cut(s)	127, 234, 404, 475, 1453, 1574, 1849, 2293, 2294
AsuC2I	CCSGG	3 cut(s)	34, 755, 1595
AsuHPI	GGTGA	3 cut(s)	82, 308, 1728
AvaI	CYCGRG	1 cut(s)	1146
AvaII	GGWCC	5 cut(s)	127, 234, 404, 1453, 1849
AxyI	CCTNAGG	1 cut(s)	1440
BaeGI	GKGCMC	2 cut(s)	2205, 2297
BaeI	ACNNNNGTAYC	2 cut(s)	231, 264
BamHI	GGATCC	1 cut(s)	2131
BanI	GGYRCC	6 cut(s)	247, 659, 1597, 1723, 2091, 2110
BanII	GRGCYC	2 cut(s)	387, 2297
BarI	GAAGNNNNNNTAC	2 cut(s)	1656, 1688
BbsI	GAAGAC	1 cut(s)	2223
Bbv12I	GWGCWC	1 cut(s)	387
BbvI	GCAGC	7 cut(s)	165, 383, 661, 955, 1357, 1433, 2037
BceAI	ACGGC	1 cut(s)	489
BciT130I	CCWGG	3 cut(s)	1085, 1602, 2168
BciVI	GTATCC	2 cut(s)	48, 1206
BcnI	CCSGG	3 cut(s)	34, 755, 1595
BcoDI	GTCTC	2 cut(s)	800, 1552
BcuI	ACTAGT	1 cut(s)	481
BfaI	CTAG	3 cut(s)	159, 482, 1959
BfmI	CTRYAG	4 cut(s)	647, 2042, 2136, 2287
BfoI	RGCGCY	2 cut(s)	504, 2309
BfuAI	ACCTGC	2 cut(s)	1033, 1860
BfuI	GTATCC	2 cut(s)	48, 1206
BglII	AGATCT	2 cut(s)	880, 1188
BisI	GCNGC	8 cut(s)	179, 334, 372, 650, 969, 1371, 1422, 2026
BlsI	GCNGC	8 cut(s)	180, 335, 373, 651, 970, 1372, 1423, 2027
BmcAI	AGTACT	1 cut(s)	136
Bme1390I	CCNGG	6 cut(s)	34, 755, 1085, 1595, 1602, 2168
Bme18I	GGWCC	5 cut(s)	127, 234, 404, 1453, 1849
BmeT110I	CYCGRG	1 cut(s)	1146
BmgT120I	GGNCC	9 cut(s)	127, 234, 404, 475, 1453, 1574, 1849, 2293, 2294
BmrFI	CCNGG	6 cut(s)	34, 755, 1085, 1595, 1602, 2168
BmsI	GCATC	1 cut(s)	1076
BpiI	GAAGAC	1 cut(s)	2223
BplI	GAGNNNNNCTC	3 cut(s)	28, 371, 403
BpmI	CTGGAG	1 cut(s)	519
BpuEI	CTTGAG	2 cut(s)	242, 1894
BpuMI	CCSGG	3 cut(s)	34, 755, 1595
Bsa29I	ATCGAT	1 cut(s)	905
BsaHI	GRCGYC	1 cut(s)	26
BsaJI	CCNNGG	4 cut(s)	109, 1132, 1582, 2172
BsaXI	ACNNNNNCTCC	4 cut(s)	510, 540, 1437, 1467
Bsc4I	CCNNNNNNNGG	7 cut(s)	115, 484, 1445, 1583, 1607, 2173, 2302
Bse1I	ACTGG	3 cut(s)	814, 862, 1335
Bse21I	CCTNAGG	1 cut(s)	1440
Bse3DI	GCAATG	1 cut(s)	1127
BseBI	CCWGG	3 cut(s)	1085, 1602, 2168
BseCI	ATCGAT	1 cut(s)	905
BseDI	CCNNGG	4 cut(s)	109, 1132, 1582, 2172
BseGI	GGATG	6 cut(s)	1454, 1837, 1878, 1947, 2016, 2349
BseLI	CCNNNNNNNGG	7 cut(s)	115, 484, 1445, 1583, 1607, 2173, 2302
BseMI	GCAATG	1 cut(s)	1127
BseMII	CTCAG	5 cut(s)	145, 186, 402, 1454, 1635
BseNI	ACTGG	3 cut(s)	814, 862, 1335
BseRI	GAGGAG	8 cut(s)	42, 132, 395, 596, 596, 2084, 2101, 2236
BseSI	GKGCMC	2 cut(s)	2205, 2297
BseXI	GCAGC	7 cut(s)	165, 383, 661, 955, 1357, 1433, 2037
BsgI	GTGCAG	2 cut(s)	164, 1520
Bsh1236I	CGCG	1 cut(s)	1513
BshFI	GGCC	9 cut(s)	476, 636, 729, 1042, 1575, 1611, 1761, 2171, 2295
BshNI	GGYRCC	6 cut(s)	247, 659, 1597, 1723, 2091, 2110
BshVI	ATCGAT	1 cut(s)	905
BsiHKAI	GWGCWC	1 cut(s)	387
BsiHKCI	CYCGRG	1 cut(s)	1146
BsiSI	CCGG	5 cut(s)	8, 34, 755, 1594, 1762
BslFI	GGGAC	5 cut(s)	140, 319, 415, 1439, 1853
BslI	CCNNNNNNNGG	7 cut(s)	115, 484, 1445, 1583, 1607, 2173, 2302
BsmAI	GTCTC	2 cut(s)	800, 1552
BsmFI	GGGAC	5 cut(s)	140, 319, 415, 1439, 1853
BsmI	GAATGC	2 cut(s)	923, 1679
BsnI	GGCC	9 cut(s)	476, 636, 729, 1042, 1575, 1611, 1761, 2171, 2295
BsoBI	CYCGRG	1 cut(s)	1146
Bsp120I	GGGCCC	1 cut(s)	2293
Bsp1286I	GDGCHC	3 cut(s)	387, 2205, 2297
Bsp1407I	TGTACA	1 cut(s)	732
Bsp143I	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
BspACI	CCGC	3 cut(s)	334, 1513, 1946
BspANI	GGCC	9 cut(s)	476, 636, 729, 1042, 1575, 1611, 1761, 2171, 2295
BspCNI	CTCAG	5 cut(s)	144, 187, 401, 1453, 1634
BspDI	ATCGAT	1 cut(s)	905
BspFNI	CGCG	1 cut(s)	1513
BspMAI	CTGCAG	4 cut(s)	651, 2046, 2140, 2291
BspMI	ACCTGC	2 cut(s)	1033, 1860
BspPI	GGATC	4 cut(s)	1216, 2126, 2139, 2158
BspQI	GCTCTTC	1 cut(s)	2142
BspT107I	GGYRCC	6 cut(s)	247, 659, 1597, 1723, 2091, 2110
BsrDI	GCAATG	1 cut(s)	1127
BsrGI	TGTACA	1 cut(s)	732
BsrI	ACTGG	3 cut(s)	814, 862, 1335
BssECI	CCNNGG	4 cut(s)	109, 1132, 1582, 2172
BssMI	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
BssNI	GRCGYC	1 cut(s)	26
BssT1I	CCWWGG	4 cut(s)	109, 1132, 1582, 2172
Bst2UI	CCWGG	3 cut(s)	1085, 1602, 2168
Bst4CI	ACNGT	2 cut(s)	404, 1007
Bst6I	CTCTTC	1 cut(s)	2142
BstACI	GRCGYC	1 cut(s)	26
BstAPI	GCANNNNNTGC	1 cut(s)	2186
BstAUI	TGTACA	1 cut(s)	732
BstC8I	GCNNGC	4 cut(s)	369, 505, 615, 1291
BstDEI	CTNAG	6 cut(s)	131, 195, 388, 1440, 1621, 2276
BstENI	CCTNNNNNAGG	2 cut(s)	1443, 1605
BstF5I	GGATG	6 cut(s)	1454, 1837, 1878, 1947, 2016, 2349
BstFNI	CGCG	1 cut(s)	1513
BstH2I	RGCGCY	2 cut(s)	504, 2309
BstHHI	GCGC	3 cut(s)	316, 503, 2308
BstKTI	GATC	7 cut(s)	883, 1191, 1224, 1534, 1778, 2134, 2166
BstMAI	GTCTC	2 cut(s)	800, 1552
BstMBI	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
BstMWI	GCNNNNNNNGC	6 cut(s)	322, 875, 1064, 1980, 2177, 2186
BstNI	CCWGG	3 cut(s)	1085, 1602, 2168
BstSCI	CCNGG	6 cut(s)	32, 753, 1083, 1593, 1600, 2166
BstSFI	CTRYAG	4 cut(s)	647, 2042, 2136, 2287
BstSLI	GKGCMC	2 cut(s)	2205, 2297
BstUI	CGCG	1 cut(s)	1513
BstV1I	GCAGC	7 cut(s)	165, 383, 661, 955, 1357, 1433, 2037
BstV2I	GAAGAC	1 cut(s)	2223
BstX2I	RGATCY	3 cut(s)	880, 1188, 2131
BstXI	CCANNNNNNTGG	1 cut(s)	244
BstYI	RGATCY	3 cut(s)	880, 1188, 2131
Bsu15I	ATCGAT	1 cut(s)	905
Bsu36I	CCTNAGG	1 cut(s)	1440
BsuI	GTATCC	2 cut(s)	48, 1206
BsuRI	GGCC	9 cut(s)	476, 636, 729, 1042, 1575, 1611, 1761, 2171, 2295
BsuTUI	ATCGAT	1 cut(s)	905
BtsCI	GGATG	6 cut(s)	1454, 1837, 1878, 1947, 2016, 2349
BtsI	GCAGTG	3 cut(s)	894, 1899, 1990
BtsIMutI	CAGTG	5 cut(s)	894, 1328, 1899, 1990, 2064
BveI	ACCTGC	2 cut(s)	1033, 1860
Cac8I	GCNNGC	4 cut(s)	369, 505, 615, 1291
CfoI	GCGC	3 cut(s)	316, 503, 2308
Cfr13I	GGNCC	9 cut(s)	127, 234, 404, 475, 1453, 1574, 1849, 2293, 2294
ClaI	ATCGAT	1 cut(s)	905
CseI	GACGC	1 cut(s)	34
CviAII	CATG	6 cut(s)	66, 338, 508, 958, 1127, 1294
DdeI	CTNAG	6 cut(s)	131, 195, 388, 1440, 1621, 2276
DpnI	GATC	7 cut(s)	882, 1190, 1223, 1533, 1777, 2133, 2165
DpnII	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
DraI	TTTAAA	1 cut(s)	438
DraIII	CACNNNGTG	1 cut(s)	1690
EaeI	YGGCCR	1 cut(s)	1759
Eam1104I	CTCTTC	1 cut(s)	2142
EarI	CTCTTC	1 cut(s)	2142
Ecl136II	GAGCTC	1 cut(s)	385
Eco130I	CCWWGG	4 cut(s)	109, 1132, 1582, 2172
Eco147I	AGGCCT	1 cut(s)	1611
Eco24I	GRGCYC	2 cut(s)	387, 2297
Eco32I	GATATC	2 cut(s)	711, 1923
Eco47I	GGWCC	5 cut(s)	127, 234, 404, 1453, 1849
Eco53kI	GAGCTC	1 cut(s)	385
Eco57I	CTGAAG	3 cut(s)	885, 1761, 2261
Eco81I	CCTNAGG	1 cut(s)	1440
Eco88I	CYCGRG	1 cut(s)	1146
EcoICRI	GAGCTC	1 cut(s)	385
EcoNI	CCTNNNNNAGG	2 cut(s)	1443, 1605
EcoO109I	RGGNCCY	5 cut(s)	127, 1453, 1849, 2293, 2294
EcoRII	CCWGG	3 cut(s)	1083, 1600, 2166
EcoRV	GATATC	2 cut(s)	711, 1923
EcoT14I	CCWWGG	4 cut(s)	109, 1132, 1582, 2172
EcoT38I	GRGCYC	2 cut(s)	387, 2297
ErhI	CCWWGG	4 cut(s)	109, 1132, 1582, 2172
FaeI	CATG	6 cut(s)	69, 341, 511, 961, 1130, 1297
FalI	AAGNNNNNCTT	2 cut(s)	251, 283
FaqI	GGGAC	5 cut(s)	140, 319, 415, 1439, 1853
FatI	CATG	6 cut(s)	65, 337, 507, 957, 1126, 1293
Fnu4HI	GCNGC	8 cut(s)	179, 334, 372, 650, 969, 1371, 1422, 2026
FokI	GGATG	6 cut(s)	1461, 1844, 1865, 1954, 2003, 2356
FriOI	GRGCYC	2 cut(s)	387, 2297
Fsp4HI	GCNGC	8 cut(s)	179, 334, 372, 650, 969, 1371, 1422, 2026
FspBI	CTAG	3 cut(s)	159, 482, 1959
GlaI	GCGC	3 cut(s)	315, 502, 2307
GluI	GCNGC	8 cut(s)	179, 334, 372, 650, 969, 1371, 1422, 2026
GsuI	CTGGAG	1 cut(s)	519
HaeII	RGCGCY	2 cut(s)	504, 2309
HaeIII	GGCC	9 cut(s)	476, 636, 729, 1042, 1575, 1611, 1761, 2171, 2295
HapII	CCGG	5 cut(s)	8, 34, 755, 1594, 1762
HgaI	GACGC	1 cut(s)	34
HhaI	GCGC	3 cut(s)	316, 503, 2308
Hin1I	GRCGYC	1 cut(s)	26
Hin1II	CATG	6 cut(s)	69, 341, 511, 961, 1130, 1297
Hin6I	GCGC	3 cut(s)	314, 501, 2306
HinP1I	GCGC	3 cut(s)	314, 501, 2306
HincII	GTYRAC	1 cut(s)	591
HindII	GTYRAC	1 cut(s)	591
HindIII	AAGCTT	1 cut(s)	2246
HinfI	GANTC	4 cut(s)	11, 73, 1016, 2012
HpaI	GTTAAC	1 cut(s)	591
HpaII	CCGG	5 cut(s)	8, 34, 755, 1594, 1762
HphI	GGTGA	3 cut(s)	82, 308, 1728
Hpy166II	GTNNAC	5 cut(s)	353, 527, 591, 1162, 1988
Hpy188I	TCNGA	6 cut(s)	196, 1624, 1755, 2037, 2241, 2360
Hpy188III	TCNNGA	8 cut(s)	232, 850, 989, 1199, 1253, 1312, 1882, 2020
Hpy8I	GTNNAC	5 cut(s)	353, 527, 591, 1162, 1988
Hpy99I	CGWCG	2 cut(s)	28, 290
HpyAV	CCTTC	4 cut(s)	293, 417, 1932, 2209
HpyCH4III	ACNGT	2 cut(s)	404, 1007
HpyCH4IV	ACGT	4 cut(s)	150, 264, 1494, 2121
HpyF10VI	GCNNNNNNNGC	6 cut(s)	322, 875, 1064, 1980, 2177, 2186
HpyF3I	CTNAG	6 cut(s)	131, 195, 388, 1440, 1621, 2276
HpySE526I	ACGT	4 cut(s)	150, 264, 1494, 2121
Hsp92I	GRCGYC	1 cut(s)	26
Hsp92II	CATG	6 cut(s)	69, 341, 511, 961, 1130, 1297
HspAI	GCGC	3 cut(s)	314, 501, 2306
KflI	GGGWCCC	1 cut(s)	1453
KpnI	GGTACC	2 cut(s)	251, 2114
KspAI	GTTAAC	1 cut(s)	591
Kzo9I	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
LguI	GCTCTTC	1 cut(s)	2142
LmnI	GCTCC	3 cut(s)	4, 382, 1033
Lsp1109I	GCAGC	7 cut(s)	165, 383, 661, 955, 1357, 1433, 2037
LweI	GCATC	1 cut(s)	1076
MaeI	CTAG	3 cut(s)	159, 482, 1959
MaeII	ACGT	4 cut(s)	150, 264, 1494, 2121
MaeIII	GTNAC	4 cut(s)	465, 567, 574, 1663
MalI	GATC	7 cut(s)	882, 1190, 1223, 1533, 1777, 2133, 2165
MboI	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
MboII	GAAGA	9 cut(s)	220, 836, 1528, 1664, 1754, 2159, 2223, 2254, 2293
MfeI	CAATTG	1 cut(s)	1071
MflI	RGATCY	3 cut(s)	880, 1188, 2131
MhlI	GDGCHC	3 cut(s)	387, 2205, 2297
MluCI	AATT	7 cut(s)	18, 540, 1071, 1203, 1207, 1266, 1904
MlyI	GAGTC	1 cut(s)	5
MmeI	TCCRAC	2 cut(s)	803, 1057
MroXI	GAANNNNTTC	1 cut(s)	1749
MseI	TTAA	8 cut(s)	224, 437, 590, 1206, 1265, 1431, 1553, 2310
MslI	CAYNNNNRTG	5 cut(s)	242, 1179, 1478, 1883, 2208
MspA1I	CMGCKG	1 cut(s)	374
MspI	CCGG	5 cut(s)	8, 34, 755, 1594, 1762
MspR9I	CCNGG	6 cut(s)	34, 755, 1085, 1595, 1602, 2168
MunI	CAATTG	1 cut(s)	1071
Mva1269I	GAATGC	2 cut(s)	923, 1679
MvaI	CCWGG	3 cut(s)	1085, 1602, 2168
MvnI	CGCG	1 cut(s)	1513
MwoI	GCNNNNNNNGC	6 cut(s)	322, 875, 1064, 1980, 2177, 2186
NciI	CCSGG	3 cut(s)	34, 755, 1595
NdeII	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
NlaIII	CATG	6 cut(s)	69, 341, 511, 961, 1130, 1297
PceI	AGGCCT	1 cut(s)	1611
PciSI	GCTCTTC	1 cut(s)	2142
PcsI	WCGNNNNNNNCGW	1 cut(s)	52
PctI	GAATGC	2 cut(s)	923, 1679
PdmI	GAANNNNTTC	1 cut(s)	1749
PfeI	GAWTC	3 cut(s)	73, 1016, 2012
PflMI	CCANNNNNTGG	1 cut(s)	1583
PkrI	GCNGC	8 cut(s)	180, 335, 373, 651, 970, 1372, 1423, 2027
PleI	GAGTC	1 cut(s)	5
PpsI	GAGTC	1 cut(s)	5
PpuMI	RGGWCCY	3 cut(s)	127, 1453, 1849
PshBI	ATTAAT	1 cut(s)	1206
PsiI	TTATAA	2 cut(s)	1094, 1952
Psp124BI	GAGCTC	1 cut(s)	387
Psp5II	RGGWCCY	3 cut(s)	127, 1453, 1849
Psp6I	CCWGG	3 cut(s)	1083, 1600, 2166
PspGI	CCWGG	3 cut(s)	1083, 1600, 2166
PspOMI	GGGCCC	1 cut(s)	2293
PspPI	GGNCC	9 cut(s)	127, 234, 404, 475, 1453, 1574, 1849, 2293, 2294
PspPPI	RGGWCCY	3 cut(s)	127, 1453, 1849
PsrI	GAACNNNNNNTAC	2 cut(s)	1701, 1733
PstI	CTGCAG	4 cut(s)	651, 2046, 2140, 2291
PsuI	RGATCY	3 cut(s)	880, 1188, 2131
PvuII	CAGCTG	1 cut(s)	374
RseI	CAYNNNNRTG	5 cut(s)	242, 1179, 1478, 1883, 2208
SacI	GAGCTC	1 cut(s)	387
SapI	GCTCTTC	1 cut(s)	2142
SaqAI	TTAA	8 cut(s)	224, 437, 590, 1206, 1265, 1431, 1553, 2310
SatI	GCNGC	8 cut(s)	179, 334, 372, 650, 969, 1371, 1422, 2026
Sau3AI	GATC	7 cut(s)	880, 1188, 1221, 1531, 1775, 2131, 2163
Sau96I	GGNCC	9 cut(s)	127, 234, 404, 475, 1453, 1574, 1849, 2293, 2294
ScaI	AGTACT	1 cut(s)	136
SchI	GAGTC	1 cut(s)	5
ScrFI	CCNGG	6 cut(s)	34, 755, 1085, 1595, 1602, 2168
SduI	GDGCHC	3 cut(s)	387, 2205, 2297
SfaNI	GCATC	1 cut(s)	1076
SfcI	CTRYAG	4 cut(s)	647, 2042, 2136, 2287
SinI	GGWCC	5 cut(s)	127, 234, 404, 1453, 1849
SmiMI	CAYNNNNRTG	5 cut(s)	242, 1179, 1478, 1883, 2208
SmlI	CTYRAG	2 cut(s)	257, 1909
SmoI	CTYRAG	2 cut(s)	257, 1909
SpeI	ACTAGT	1 cut(s)	481
Sse9I	AATT	7 cut(s)	18, 540, 1071, 1203, 1207, 1266, 1904
SseBI	AGGCCT	1 cut(s)	1611
SsiI	CCGC	3 cut(s)	334, 1513, 1946
SspI	AATATT	2 cut(s)	717, 1650
SspMI	CTAG	3 cut(s)	159, 482, 1959
SstI	GAGCTC	1 cut(s)	387
StuI	AGGCCT	1 cut(s)	1611
StyD4I	CCNGG	6 cut(s)	32, 753, 1083, 1593, 1600, 2166
StyI	CCWWGG	4 cut(s)	109, 1132, 1582, 2172
TaaI	ACNGT	2 cut(s)	404, 1007
TaiI	ACGT	4 cut(s)	153, 267, 1497, 2124
TaqI	TCGA	5 cut(s)	23, 851, 905, 1970, 2334
TasI	AATT	7 cut(s)	18, 540, 1071, 1203, 1207, 1266, 1904
TatI	WGTACW	4 cut(s)	134, 526, 732, 1410
TauI	GCSGC	1 cut(s)	336
TfiI	GAWTC	3 cut(s)	73, 1016, 2012
Tru1I	TTAA	8 cut(s)	224, 437, 590, 1206, 1265, 1431, 1553, 2310
Tru9I	TTAA	8 cut(s)	224, 437, 590, 1206, 1265, 1431, 1553, 2310
TscAI	CASTG	5 cut(s)	901, 1335, 1899, 1990, 2064
TseI	GCWGC	7 cut(s)	178, 371, 649, 968, 1370, 1421, 2025
TspDTI	ATGAA	6 cut(s)	54, 399, 1387, 1462, 1867, 2223
TspGWI	ACGGA	4 cut(s)	484, 712, 791, 1296
TspRI	CASTG	5 cut(s)	901, 1335, 1899, 1990, 2064
Van91I	CCANNNNNTGG	1 cut(s)	1583
VpaK11BI	GGWCC	5 cut(s)	127, 234, 404, 1453, 1849
VspI	ATTAAT	1 cut(s)	1206
XagI	CCTNNNNNAGG	2 cut(s)	1443, 1605
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1206
XmnI	GAANNNNTTC	1 cut(s)	1749
XspI	CTAG	3 cut(s)	159, 482, 1959
ZrmI	AGTACT	1 cut(s)	136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.