Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009766

Physical Location & Seq

Genomic Coordinates Forward (+)

285841 .. 302929

Gene Structure 17089 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1002800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3066 bp

ATGGCTTCTACCGCTGCGGCGTTGTTTCTGTTCACGGCGACAACGGCGGTCTTATTGGCTGGGTTACCGGGAGCAAAATCGGAGTACATAGCTTATAACACGTCGGCGGGAATTGTCCCCGGAAAAATCAATGTTCATTTAGTTCCCCACTCTCACGACGACGTCGGGTGGCTTAAGACCGTTGACCAGTACTTCGTAGGCGCCAACAACTCAATTCGCGGCGCTTGCGTTCAGAACGTCCTCGACTCTGTAATTTCGGCCTTGCTGGAGGAGAAGAATCGGAAGTTCATCTATGTCGAGATTGCATTCTTTCAGAGATGGTGGAGACAGCAAAGCCCGGCCTTGAAGGCTAAAGTCAAGGAGCTAGTCAGCTCTGGTCAGCTCGAGTTCATAAATGGAGGAATGTGTATGCACGACGAGGCCACCCCGCATTACATGGACTTGATTGATCAGACAACTTTAGGCCATCAATTTATTCTGAAAGAATTTGGTCAGGTTCCTCGAATCGGCTGGCAGATTGACCCTTTTGGCCACTCTGCTGTTCAAGCTTACCTGCTTGGTGCTGAGTTAGGATTCGATTCCCTCTTTTTCGCTCGAATAGATTACCAAGATAGAGCTAAGCGACTCCGGGATAAGACTCTTGAATTTATATGGCAGGGTTCTAGATCCCTTGCTTCATCTTCGCAGATTTTCACAGGCATTTTCCCCAGACATTATGATCCCCCTGATGGTTTCGTGTTTGAAATAAATGATGTGTCACCCCCTATTCAGGATGACGTTCTCATGTTCGACTACAATGTTCAAGAGCGAGTCAACGACTTTGTAGCAGCTGCTTTAGCACAGGCTAATGTGACAAGAACAAACCATATTATGTGGAACATGGGGACAGATTTCCGCTATCAATATGCATATTCATGGTTCAGACAGATGGACAAATTCATTCATTATGTCAATCAGGATGGGCGTGTCAATGCATTGTATTCAACCCCATCAATCTACACTGATGCAAAATATGCGACAAATGAACAATGGCCTCTAAAAACTGATGATTTCTTCCCGTATGCAGATAAACCTAATGCATATTGGACTGGTTATTTTACAAGTCGTCCAGCCCTTAAAGGTTATGTCAGAAGTATGAGTGGTTACTATCAGGCAGCTAGGCAATTGGAATTCTTTCGAGGCAGAAGTGATTCTGGACCAAACACTGATGCATTAGCTGATGCTTTGGCTATTGCTCAACATCATGATGCAGTTAGTGGTACAGAAAGACAGCATGTTGCTGCTGATTATGCAATGCGACTGTCAATAGGCTATTTACAGGCTGAAAAGGTGGTTGCTTCCTCTCTTGCTTACCTTTCCAAGTCAGAATCAAGATCCGGACAGGAGCAAACTGTCACAAATTTTCAACAGTGTCCTCTCCTAAATATTAGTTATTGTCCTCCATCAGAAGCTGTCTTGTCTAATGGAAAAAACTTGGTTGTTGTTGTCTACAATCCACTTGGTTGGAAGAGAGAAGAAGTGATACGTATCCCCATTTCCAATGAAAAGGTTACTGTTCAGGATTCCAGTGGAACAGAAATTGAAGCTCAGCTTCTACCCCCATCAAACGCTTCTTTGAGGTTAAGGAGTTACTATGTTAGAGCATATTTGGGTAAAACTCCAGGTGAACCACCCAAGTATTGGCTAGCATTTTCAGTGACAGTACCACCAATTGGTTTCAGTTCTTACATTGTCTCAATTGCAAAACAGACAGGTAGTGGTTCAACTGTTTCCAAAGTATACTCGTCAGAGAGAAGTATGAATAAGACCATAGAAGTTGGGAAAGGAAGTTTAAAGCTGCATTATTCTGCAGATGAAGGAAAACTTGCTCGTTATGTTAATAGCAGAAGTCAGGTTACATCATCTGCTGAACAATCATATAGTTATTATAGTGGAAATGACGGGACTGACAAAGATCCTCAGGCGTCTGGGGCATATGTGTTTCGACCAAATAGCACAGTTCCTATAAAGTCTGAACGGAAGGTTCCTCTAATTGTCATGCGGGGTCCAGTTCTAGACGAAGTACATCAACAGCTCAATCCATGGGTATCTCAGATTACAAGGGTGTACAAGCAAAAGGATCATGCTGAAATTGAGTTCACTATCGGGCCTATACCTGCGGATGATGGGATTGGGAAGGAAATCACAACTCAAATTACAACAGCCGTGAAGACCAATAAGACATTCTACACAGATTCTAACGGCCGGGACTTCATTAAACGGATTCGAGATTTCAGGACAGATTGGGACCTACAAGTGAACCAACCAATAGCTGGAAACTATTACCCTATTAATCTTGGAATTTATGTTCAAGATGGCAGTACAGAAGTTTCAGTCTTGGTTGATCGTGCAGTGGGAGGAACTAGCTTGGTAGATGGTCAAATAGAGCTGATGCTCCACAGGAGGTTGCTTCATGATGATACCCGAGGTGTTGGTGAGGTACTGAATGAAACAGCTTGCATCCCTGAGAAGTGTGAAGGTTTAACTATCCAAGGGAAGTTTTACGTGAGGATCGATAATCTTGGAGAAGGTGCTAAGTGGCGTCGCACGCTTGGGCAAGAGATAAATTCTCCGCTTCTTTTGGCCTTCACAGAACAGGATGAAAATGATTGGGTGAATTCTCATGCATTGACTTTCTCTGGAATTGATCCTTCCTATGCTTTACCAAACAATACTGCTGTCATAACACTCCAGGAACTTGAAAATCGGGAAGTACTCCTCCGGCTGGCCCACCTATATGAGATTGGAGAAGACAAAGACTATTCTGTACTGGCGAATGTGGAACTAAAGAAGTTGTTCCCAACAAAGAAGATCAGCAAAGTGACAGAGATGAGTTTGTCGGCCAATCAAGAAAGATCTGAGATGGAGAAGAGGAGGTTAGTATGGAAAGTAGAAGGTTCCGGCCAAGAGTTCAAGGTGGTGAGGGGTGGACCTGTCGACCCTGCAAAACTGGTGGTGGAACTAGCTCCAATGGAAATACGCACTTTCATTGTCGACTTTGATCACCTCCGTATGTATGGTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005774 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009505 GO:0009987 GO:0015923 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030312 GO:0031090 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1022

Amino Acids

114.66

Weight (kDa)

6.08

Isoelectric Point (pI)

40.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	44 - 355	7.1e-85	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	360 - 452	7.6e-20	Alpha mannosidase middle domain
Laman-like_dom	PF21260	557 - 578	1.1e-06	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	608 - 822	3.5e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	906 - 1009	8.7e-07	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26720 AT3G26720 AT3G26720 AT3G26720 AT3G26720
fragaria_vesca	FvH4_6g54280 FvH4_6g54280 FvH4_6g54280 FvH4_6g54280
malus_domestica	MD09G1002800.v1.1
prunus_persica	Prupe.3G313400_v2.0.a1 Prupe.3G313400_v2.0.a1
pyrus_communis	pycom111g00230
rosa_chinensis	RchiOBHm_Chr2g0176491
rosa_laevigata	RLG00000022417
rosa_roxburghii	Rroxscaffold_2G00076340
rosa_rugosa	Rorug02G0595100
rosa_samantha	Rh2AG673400 Rh2BG684700 Rh2DG698300
rosa_wichuraiana	Rw2G055230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	96
AatII	GACGTC	1 cut(s)	165
Acc36I	ACCTGC	2 cut(s)	561, 2164
AccB1I	GGYRCC	1 cut(s)	200
AccB7I	CCANNNNNTGG	3 cut(s)	1680, 1712, 2312
AccI	GTMKAC	4 cut(s)	1488, 1779, 2976, 3033
AccII	CGCG	1 cut(s)	219
AccIII	TCCGGA	1 cut(s)	1376
AclWI	GGATC	7 cut(s)	660, 713, 1368, 1949, 2127, 2558, 2681
AcoI	YGGCCR	4 cut(s)	529, 2242, 2880, 2941
AcsI	RAATTY	8 cut(s)	485, 644, 935, 1169, 1399, 2340, 2605, 2656
AcyI	GRCGYC	4 cut(s)	162, 201, 1964, 2581
AfiI	CCNNNNNNNGG	7 cut(s)	506, 728, 769, 1680, 1712, 2312, 2764
AflII	CTTAAG	1 cut(s)	173
AflIII	ACRYGT	1 cut(s)	99
AjiI	CACGTC	1 cut(s)	102
AjnI	CCWGG	2 cut(s)	1660, 2730
AjuI	GAANNNNNNNTTGG	2 cut(s)	2207, 2239
AloI	GAACNNNNNNTCC	2 cut(s)	2331, 2363
Alw26I	GTCTC	2 cut(s)	319, 1738
AlwI	GGATC	7 cut(s)	660, 713, 1368, 1949, 2127, 2558, 2681
AlwNI	CAGNNNCTG	1 cut(s)	1451
Ama87I	CYCGRG	2 cut(s)	383, 2463
Aor13HI	TCCGGA	1 cut(s)	1376
ApeKI	GCWGC	6 cut(s)	14, 827, 830, 1154, 1280, 1837
ApoI	RAATTY	8 cut(s)	485, 644, 935, 1169, 1399, 2340, 2605, 2656
ArsI	GACNNNNNNTTYG	2 cut(s)	176, 208
AseI	ATTAAT	1 cut(s)	2331
Asp700I	GAANNNNTTC	3 cut(s)	1173, 1189, 2834
AspLEI	GCGC	2 cut(s)	203, 224
AspS9I	GGNCC	6 cut(s)	1196, 2045, 2147, 2287, 2767, 2969
AsuC2I	CCSGG	5 cut(s)	69, 120, 338, 629, 2246
AsuHPI	GGTGA	6 cut(s)	750, 1676, 2486, 2665, 2971, 3035
AsuNHI	GCTAGC	1 cut(s)	1684
AvaI	CYCGRG	2 cut(s)	383, 2463
AvaII	GGWCC	4 cut(s)	1196, 2045, 2287, 2969
AxyI	CCTNAGG	1 cut(s)	1959
BalI	TGGCCA	1 cut(s)	531
BanI	GGYRCC	1 cut(s)	200
BarI	GAAGNNNNNNTAC	4 cut(s)	1506, 1538, 1879, 1911
BbsI	GAAGAC	2 cut(s)	2216, 2796
BbvI	GCAGC	5 cut(s)	817, 839, 1166, 1267, 1824
BceAI	ACGGC	4 cut(s)	51, 60, 2189, 2257
BciT130I	CCWGG	2 cut(s)	1662, 2732
BciVI	GTATCC	1 cut(s)	1538
BclI	TGATCA	2 cut(s)	448, 3040
BcnI	CCSGG	5 cut(s)	69, 120, 338, 629, 2246
BcoDI	GTCTC	2 cut(s)	319, 1738
BfaI	CTAG	7 cut(s)	365, 663, 1158, 1685, 2054, 2403, 3002
BfmI	CTRYAG	1 cut(s)	1848
BfoI	RGCGCY	2 cut(s)	204, 225
BfrI	CTTAAG	1 cut(s)	173
BfuAI	ACCTGC	2 cut(s)	561, 2164
BfuI	GTATCC	1 cut(s)	1538
BglI	GCCNNNNNGGC	1 cut(s)	347
BglII	AGATCT	1 cut(s)	2894
BisI	GCNGC	8 cut(s)	15, 18, 220, 828, 831, 1155, 1281, 1838
BlpI	GCTNAGC	2 cut(s)	618, 1587
BlsI	GCNGC	8 cut(s)	16, 19, 221, 829, 832, 1156, 1282, 1839
BmcAI	AGTACT	2 cut(s)	191, 2754
Bme1390I	CCNGG	7 cut(s)	69, 120, 338, 629, 1662, 2246, 2732
Bme18I	GGWCC	4 cut(s)	1196, 2045, 2287, 2969
BmeT110I	CYCGRG	2 cut(s)	383, 2463
BmgBI	CACGTC	1 cut(s)	102
BmgT120I	GGNCC	6 cut(s)	1196, 2045, 2147, 2287, 2767, 2969
BmiI	GGNNCC	6 cut(s)	202, 498, 2025, 2046, 2288, 2938
BmrFI	CCNGG	7 cut(s)	69, 120, 338, 629, 1662, 2246, 2732
BmsI	GCATC	6 cut(s)	994, 1198, 1210, 1237, 2421, 2508
BmtI	GCTAGC	1 cut(s)	1688
BpiI	GAAGAC	2 cut(s)	2216, 2796
BpmI	CTGGAG	3 cut(s)	287, 1644, 2714
Bpu1102I	GCTNAGC	2 cut(s)	618, 1587
BpuMI	CCSGG	5 cut(s)	69, 120, 338, 629, 2246
Bsa29I	ATCGAT	1 cut(s)	2553
BsaAI	YACGTR	2 cut(s)	1526, 2545
BsaBI	GATNNNNATC	1 cut(s)	1526
BsaHI	GRCGYC	4 cut(s)	162, 201, 1964, 2581
BsaJI	CCNNGG	4 cut(s)	118, 2081, 2464, 2530
BsaWI	WCCGGW	1 cut(s)	1376
Bsc4I	CCNNNNNNNGG	7 cut(s)	506, 728, 769, 1680, 1712, 2312, 2764
Bse1I	ACTGG	6 cut(s)	187, 1093, 1566, 2048, 2814, 2994
Bse21I	CCTNAGG	1 cut(s)	1959
Bse3DI	GCAATG	1 cut(s)	1299
Bse8I	GATNNNNATC	1 cut(s)	1526
BseAI	TCCGGA	1 cut(s)	1376
BseBI	CCWGG	2 cut(s)	1662, 2732
BseCI	ATCGAT	1 cut(s)	2553
BseDI	CCNNGG	4 cut(s)	118, 2081, 2464, 2530
BseGI	GGATG	5 cut(s)	778, 964, 2167, 2499, 2644
BseJI	GATNNNNATC	1 cut(s)	1526
BseLI	CCNNNNNNNGG	7 cut(s)	506, 728, 769, 1680, 1712, 2312, 2764
BseMI	GCAATG	1 cut(s)	1299
BseMII	CTCAG	6 cut(s)	555, 1601, 1973, 2105, 2496, 2889
BseNI	ACTGG	6 cut(s)	187, 1093, 1566, 2048, 2814, 2994
BseRI	GAGGAG	3 cut(s)	284, 2747, 2926
BseX3I	CGGCCG	1 cut(s)	2242
BseXI	GCAGC	5 cut(s)	817, 839, 1166, 1267, 1824
BseYI	CCCAGC	1 cut(s)	59
BsgI	GTGCAG	1 cut(s)	2409
Bsh1236I	CGCG	1 cut(s)	219
Bsh1285I	CGRYCG	1 cut(s)	2245
BshNI	GGYRCC	1 cut(s)	200
BshVI	ATCGAT	1 cut(s)	2553
BsiEI	CGRYCG	1 cut(s)	2245
BsiHKCI	CYCGRG	2 cut(s)	383, 2463
BsiSI	CCGG	8 cut(s)	68, 120, 338, 628, 1377, 2245, 2761, 2940
BslFI	GGGAC	5 cut(s)	101, 898, 1957, 2261, 2300
BslI	CCNNNNNNNGG	7 cut(s)	506, 728, 769, 1680, 1712, 2312, 2764
BsmAI	GTCTC	2 cut(s)	319, 1738
BsmFI	GGGAC	5 cut(s)	101, 898, 1957, 2261, 2300
BsmI	GAATGC	1 cut(s)	305
BsoBI	CYCGRG	2 cut(s)	383, 2463
Bsp13I	TCCGGA	1 cut(s)	1376
Bsp1407I	TGTACA	1 cut(s)	2106
Bsp1720I	GCTNAGC	2 cut(s)	618, 1587
Bsp19I	CCATGG	1 cut(s)	2081
BspCNI	CTCAG	6 cut(s)	556, 1600, 1972, 2104, 2497, 2890
BspDI	ATCGAT	1 cut(s)	2553
BspEI	TCCGGA	1 cut(s)	1376
BspFNI	CGCG	1 cut(s)	219
BspHI	TCATGA	2 cut(s)	1243, 2452
BspLI	GGNNCC	6 cut(s)	202, 498, 2025, 2046, 2288, 2938
BspMAI	CTGCAG	1 cut(s)	1852
BspMI	ACCTGC	2 cut(s)	561, 2164
BspOI	GCTAGC	1 cut(s)	1688
BspPI	GGATC	7 cut(s)	660, 713, 1368, 1949, 2127, 2558, 2681
BspT107I	GGYRCC	1 cut(s)	200
BspTI	CTTAAG	1 cut(s)	173
BsrDI	GCAATG	1 cut(s)	1299
BsrGI	TGTACA	1 cut(s)	2106
BsrI	ACTGG	6 cut(s)	187, 1093, 1566, 2048, 2814, 2994
BssECI	CCNNGG	4 cut(s)	118, 2081, 2464, 2530
BssNAI	GTATAC	1 cut(s)	1780
BssNI	GRCGYC	4 cut(s)	162, 201, 1964, 2581
BssT1I	CCWWGG	2 cut(s)	2081, 2530
Bst1107I	GTATAC	1 cut(s)	1780
Bst2UI	CCWGG	2 cut(s)	1662, 2732
Bst4CI	ACNGT	8 cut(s)	181, 1302, 1393, 1410, 1555, 1702, 1768, 1999
Bst6I	CTCTTC	2 cut(s)	1502, 2903
BstACI	GRCGYC	4 cut(s)	162, 201, 1964, 2581
BstAFI	CTTAAG	1 cut(s)	173
BstAPI	GCANNNNNTGC	1 cut(s)	1013
BstAUI	TGTACA	1 cut(s)	2106
BstBAI	YACGTR	2 cut(s)	1526, 2545
BstC8I	GCNNGC	6 cut(s)	226, 512, 1686, 2497, 2588, 2766
BstDEI	CTNAG	8 cut(s)	564, 618, 1587, 1959, 2091, 2505, 2574, 2898
BstDSI	CCRYGG	1 cut(s)	2081
BstEII	GGTNACC	1 cut(s)	63
BstF5I	GGATG	5 cut(s)	778, 964, 2167, 2499, 2644
BstFNI	CGCG	1 cut(s)	219
BstH2I	RGCGCY	2 cut(s)	204, 225
BstHHI	GCGC	2 cut(s)	203, 224
BstMAI	GTCTC	2 cut(s)	319, 1738
BstMCI	CGRYCG	1 cut(s)	2245
BstNI	CCWGG	2 cut(s)	1662, 2732
BstNSI	RCATGY	1 cut(s)	1277
BstPI	GGTNACC	1 cut(s)	63
BstSCI	CCNGG	7 cut(s)	67, 118, 336, 627, 1660, 2244, 2730
BstSFI	CTRYAG	1 cut(s)	1848
BstSNI	TACGTA	1 cut(s)	1526
BstUI	CGCG	1 cut(s)	219
BstV1I	GCAGC	5 cut(s)	817, 839, 1166, 1267, 1824
BstV2I	GAAGAC	2 cut(s)	2216, 2796
BstX2I	RGATCY	4 cut(s)	665, 1373, 1954, 2894
BstXI	CCANNNNNNTGG	1 cut(s)	1503
BstYI	RGATCY	4 cut(s)	665, 1373, 1954, 2894
BstZ17I	GTATAC	1 cut(s)	1780
BstZI	CGGCCG	1 cut(s)	2242
Bsu15I	ATCGAT	1 cut(s)	2553
Bsu36I	CCTNAGG	1 cut(s)	1959
BsuI	GTATCC	1 cut(s)	1538
BsuTUI	ATCGAT	1 cut(s)	2553
BtgI	CCRYGG	1 cut(s)	2081
BtrI	CACGTC	1 cut(s)	102
BtsCI	GGATG	5 cut(s)	778, 964, 2167, 2499, 2644
BtsI	GCAGTG	1 cut(s)	2397
BtsIMutI	CAGTG	6 cut(s)	999, 1203, 1415, 1573, 1701, 2397
BveI	ACCTGC	2 cut(s)	561, 2164
Cac8I	GCNNGC	6 cut(s)	226, 512, 1686, 2497, 2588, 2766
CaiI	CAGNNNCTG	1 cut(s)	1451
CciI	TCATGA	2 cut(s)	1243, 2452
CfoI	GCGC	2 cut(s)	203, 224
Cfr13I	GGNCC	6 cut(s)	1196, 2045, 2147, 2287, 2767, 2969
ClaI	ATCGAT	1 cut(s)	2553
CseI	GACGC	2 cut(s)	1953, 2570
CspCI	CAANNNNNGTGG	2 cut(s)	2973, 3008
DdeI	CTNAG	8 cut(s)	564, 618, 1587, 1959, 2091, 2505, 2574, 2898
DinI	GGCGCC	1 cut(s)	202
DraI	TTTAAA	1 cut(s)	1833
EaeI	YGGCCR	4 cut(s)	529, 2242, 2880, 2941
EagI	CGGCCG	1 cut(s)	2242
Eam1104I	CTCTTC	2 cut(s)	1502, 2903
EarI	CTCTTC	2 cut(s)	1502, 2903
EclXI	CGGCCG	1 cut(s)	2242
Eco105I	TACGTA	1 cut(s)	1526
Eco130I	CCWWGG	2 cut(s)	2081, 2530
Eco47I	GGWCC	4 cut(s)	1196, 2045, 2287, 2969
Eco52I	CGGCCG	1 cut(s)	2242
Eco81I	CCTNAGG	1 cut(s)	1959
Eco88I	CYCGRG	2 cut(s)	383, 2463
Eco91I	GGTNACC	1 cut(s)	63
EcoO109I	RGGNCCY	1 cut(s)	2287
EcoO65I	GGTNACC	1 cut(s)	63
EcoRI	GAATTC	2 cut(s)	1169, 2656
EcoRII	CCWGG	2 cut(s)	1660, 2730
EcoT14I	CCWWGG	2 cut(s)	2081, 2530
EcoT22I	ATGCAT	5 cut(s)	910, 976, 1081, 1213, 2668
EgeI	GGCGCC	1 cut(s)	202
EheI	GGCGCC	1 cut(s)	202
ErhI	CCWWGG	2 cut(s)	2081, 2530
FalI	AAGNNNNNCTT	4 cut(s)	1575, 1607, 1848, 1880
FaqI	GGGAC	5 cut(s)	101, 898, 1957, 2261, 2300
FauI	CCCGC	3 cut(s)	100, 435, 2034
FauNDI	CATATG	1 cut(s)	1975
FbaI	TGATCA	2 cut(s)	448, 3040
FblI	GTMKAC	4 cut(s)	1488, 1779, 2976, 3033
Fnu4HI	GCNGC	8 cut(s)	15, 18, 220, 828, 831, 1155, 1281, 1838
FokI	GGATG	5 cut(s)	785, 971, 2174, 2486, 2651
Fsp4HI	GCNGC	8 cut(s)	15, 18, 220, 828, 831, 1155, 1281, 1838
FspBI	CTAG	7 cut(s)	365, 663, 1158, 1685, 2054, 2403, 3002
GlaI	GCGC	2 cut(s)	202, 223
GluI	GCNGC	8 cut(s)	15, 18, 220, 828, 831, 1155, 1281, 1838
GsaI	CCCAGC	1 cut(s)	63
GsuI	CTGGAG	3 cut(s)	287, 1644, 2714
HaeII	RGCGCY	2 cut(s)	204, 225
HapII	CCGG	8 cut(s)	68, 120, 338, 628, 1377, 2245, 2761, 2940
HgaI	GACGC	2 cut(s)	1953, 2570
HhaI	GCGC	2 cut(s)	203, 224
Hin1I	GRCGYC	4 cut(s)	162, 201, 1964, 2581
Hin6I	GCGC	2 cut(s)	201, 222
HinP1I	GCGC	2 cut(s)	201, 222
HincII	GTYRAC	4 cut(s)	184, 814, 2977, 3034
HindII	GTYRAC	4 cut(s)	184, 814, 2977, 3034
HindIII	AAGCTT	1 cut(s)	546
HpaII	CCGG	8 cut(s)	68, 120, 338, 628, 1377, 2245, 2761, 2940
HphI	GGTGA	6 cut(s)	750, 1676, 2486, 2665, 2971, 3035
Hpy99I	CGWCG	6 cut(s)	106, 161, 164, 167, 419, 2586
HpyAV	CCTTC	9 cut(s)	340, 1850, 2014, 2170, 2510, 2561, 2635, 2700, 2927
HpyCH4III	ACNGT	8 cut(s)	181, 1302, 1393, 1410, 1555, 1702, 1768, 1999
HpyCH4IV	ACGT	6 cut(s)	101, 162, 237, 777, 1525, 2544
HpyF3I	CTNAG	8 cut(s)	564, 618, 1587, 1959, 2091, 2505, 2574, 2898
HpySE526I	ACGT	6 cut(s)	101, 162, 237, 777, 1525, 2544
Hsp92I	GRCGYC	4 cut(s)	162, 201, 1964, 2581
HspAI	GCGC	2 cut(s)	201, 222
KasI	GGCGCC	1 cut(s)	200
Kpn2I	TCCGGA	1 cut(s)	1376
Ksp22I	TGATCA	2 cut(s)	448, 3040
LmnI	GCTCC	5 cut(s)	71, 361, 1384, 2439, 3010
Lsp1109I	GCAGC	5 cut(s)	817, 839, 1166, 1267, 1824
LweI	GCATC	6 cut(s)	994, 1198, 1210, 1237, 2421, 2508
MaeI	CTAG	7 cut(s)	365, 663, 1158, 1685, 2054, 2403, 3002
MaeII	ACGT	6 cut(s)	101, 162, 237, 777, 1525, 2544
MboII	GAAGA	9 cut(s)	286, 672, 1045, 1519, 1526, 2221, 2801, 2860, 2920
MfeI	CAATTG	3 cut(s)	1163, 1710, 1737
MflI	RGATCY	4 cut(s)	665, 1373, 1954, 2894
MlsI	TGGCCA	1 cut(s)	531
MluNI	TGGCCA	1 cut(s)	531
Mly113I	GGCGCC	1 cut(s)	201
MlyI	GAGTC	4 cut(s)	239, 618, 631, 819
MmeI	TCCRAC	1 cut(s)	1484
Mox20I	TGGCCA	1 cut(s)	531
Mph1103I	ATGCAT	5 cut(s)	910, 976, 1081, 1213, 2668
MroI	TCCGGA	1 cut(s)	1376
MroXI	GAANNNNTTC	3 cut(s)	1173, 1189, 2834
MscI	TGGCCA	1 cut(s)	531
MseI	TTAA	8 cut(s)	174, 1116, 1622, 1832, 1878, 2256, 2331, 2522
MslI	CAYNNNNRTG	3 cut(s)	913, 1245, 2775
Msp20I	TGGCCA	1 cut(s)	531
MspA1I	CMGCKG	2 cut(s)	14, 830
MspCI	CTTAAG	1 cut(s)	173
MspI	CCGG	8 cut(s)	68, 120, 338, 628, 1377, 2245, 2761, 2940
MspR9I	CCNGG	7 cut(s)	69, 120, 338, 629, 1662, 2246, 2732
MunI	CAATTG	3 cut(s)	1163, 1710, 1737
Mva1269I	GAATGC	1 cut(s)	305
MvaI	CCWGG	2 cut(s)	1662, 2732
MvnI	CGCG	1 cut(s)	219
NarI	GGCGCC	1 cut(s)	201
NciI	CCSGG	5 cut(s)	69, 120, 338, 629, 2246
NcoI	CCATGG	1 cut(s)	2081
NdeI	CATATG	1 cut(s)	1975
NheI	GCTAGC	1 cut(s)	1684
NlaIV	GGNNCC	6 cut(s)	202, 498, 2025, 2046, 2288, 2938
NmuCI	GTSAC	5 cut(s)	756, 850, 1393, 1696, 2860
NsiI	ATGCAT	5 cut(s)	910, 976, 1081, 1213, 2668
NspI	RCATGY	1 cut(s)	1277
PaeR7I	CTCGAG	1 cut(s)	383
PagI	TCATGA	2 cut(s)	1243, 2452
PcsI	WCGNNNNNNNCGW	1 cut(s)	2550
PctI	GAATGC	1 cut(s)	305
PdmI	GAANNNNTTC	3 cut(s)	1173, 1189, 2834
PfeI	GAWTC	9 cut(s)	277, 504, 573, 578, 1190, 1367, 1562, 2234, 2263
PflFI	GACNNNGTC	1 cut(s)	161
PflMI	CCANNNNNTGG	3 cut(s)	1680, 1712, 2312
PfoI	TCCNGGA	2 cut(s)	627, 2730
PkrI	GCNGC	8 cut(s)	16, 19, 221, 829, 832, 1156, 1282, 1839
PleI	GAGTC	4 cut(s)	239, 618, 631, 818
PluTI	GGCGCC	1 cut(s)	204
PpsI	GAGTC	4 cut(s)	239, 618, 631, 818
Ppu21I	YACGTR	2 cut(s)	1526, 2545
PpuMI	RGGWCCY	1 cut(s)	2287
PshBI	ATTAAT	1 cut(s)	2331
PsiI	TTATAA	1 cut(s)	96
Psp5II	RGGWCCY	1 cut(s)	2287
Psp6I	CCWGG	2 cut(s)	1660, 2730
PspEI	GGTNACC	1 cut(s)	63
PspFI	CCCAGC	1 cut(s)	59
PspGI	CCWGG	2 cut(s)	1660, 2730
PspN4I	GGNNCC	6 cut(s)	202, 498, 2025, 2046, 2288, 2938
PspPI	GGNCC	6 cut(s)	1196, 2045, 2147, 2287, 2767, 2969
PspPPI	RGGWCCY	1 cut(s)	2287
PspXI	VCTCGAGB	1 cut(s)	383
PsrI	GAACNNNNNNTAC	1 cut(s)	3043
PstI	CTGCAG	1 cut(s)	1852
PstNI	CAGNNNCTG	1 cut(s)	1451
PsuI	RGATCY	4 cut(s)	665, 1373, 1954, 2894
PsyI	GACNNNGTC	1 cut(s)	161
PvuII	CAGCTG	1 cut(s)	830
RseI	CAYNNNNRTG	3 cut(s)	913, 1245, 2775
SalI	GTCGAC	2 cut(s)	2975, 3032
SaqAI	TTAA	8 cut(s)	174, 1116, 1622, 1832, 1878, 2256, 2331, 2522
SatI	GCNGC	8 cut(s)	15, 18, 220, 828, 831, 1155, 1281, 1838
Sau96I	GGNCC	6 cut(s)	1196, 2045, 2147, 2287, 2767, 2969
ScaI	AGTACT	2 cut(s)	191, 2754
SchI	GAGTC	4 cut(s)	239, 618, 631, 819
ScrFI	CCNGG	7 cut(s)	69, 120, 338, 629, 1662, 2246, 2732
SfaNI	GCATC	6 cut(s)	994, 1198, 1210, 1237, 2421, 2508
SfcI	CTRYAG	1 cut(s)	1848
SfoI	GGCGCC	1 cut(s)	202
Sfr274I	CTCGAG	1 cut(s)	383
SinI	GGWCC	4 cut(s)	1196, 2045, 2287, 2969
SlaI	CTCGAG	1 cut(s)	383
SmiMI	CAYNNNNRTG	3 cut(s)	913, 1245, 2775
SmlI	CTYRAG	2 cut(s)	173, 383
SmoI	CTYRAG	2 cut(s)	173, 383
SnaBI	TACGTA	1 cut(s)	1526
SspDI	GGCGCC	1 cut(s)	200
SspI	AATATT	1 cut(s)	1426
SspMI	CTAG	7 cut(s)	365, 663, 1158, 1685, 2054, 2403, 3002
StyD4I	CCNGG	7 cut(s)	67, 118, 336, 627, 1660, 2244, 2730
StyI	CCWWGG	2 cut(s)	2081, 2530
TaaI	ACNGT	8 cut(s)	181, 1302, 1393, 1410, 1555, 1702, 1768, 1999
TaiI	ACGT	6 cut(s)	104, 165, 240, 780, 1528, 2547
TatI	WGTACW	7 cut(s)	84, 189, 2062, 2106, 2360, 2752, 2806
TauI	GCSGC	2 cut(s)	20, 222
TfiI	GAWTC	9 cut(s)	277, 504, 573, 578, 1190, 1367, 1562, 2234, 2263
Tru1I	TTAA	8 cut(s)	174, 1116, 1622, 1832, 1878, 2256, 2331, 2522
Tru9I	TTAA	8 cut(s)	174, 1116, 1622, 1832, 1878, 2256, 2331, 2522
TscAI	CASTG	6 cut(s)	1006, 1210, 1415, 1573, 1701, 2397
TseFI	GTSAC	5 cut(s)	756, 850, 1393, 1696, 2860
TseI	GCWGC	6 cut(s)	14, 827, 830, 1154, 1280, 1837
Tsp45I	GTSAC	5 cut(s)	756, 850, 1393, 1696, 2860
TspGWI	ACGGA	3 cut(s)	2032, 2275, 3038
TspRI	CASTG	6 cut(s)	1006, 1210, 1415, 1573, 1701, 2397
Tth111I	GACNNNGTC	1 cut(s)	161
Van91I	CCANNNNNTGG	3 cut(s)	1680, 1712, 2312
Vha464I	CTTAAG	1 cut(s)	173
VpaK11BI	GGWCC	4 cut(s)	1196, 2045, 2287, 2969
VspI	ATTAAT	1 cut(s)	2331
XapI	RAATTY	8 cut(s)	485, 644, 935, 1169, 1399, 2340, 2605, 2656
XbaI	TCTAGA	2 cut(s)	662, 2053
XceI	RCATGY	1 cut(s)	1277
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1677, 2309
XhoI	CTCGAG	1 cut(s)	383
XmiI	GTMKAC	4 cut(s)	1488, 1779, 2976, 3033
XmnI	GAANNNNTTC	3 cut(s)	1173, 1189, 2834
XspI	CTAG	7 cut(s)	365, 663, 1158, 1685, 2054, 2403, 3002
ZraI	GACGTC	1 cut(s)	163
ZrmI	AGTACT	2 cut(s)	191, 2754
Zsp2I	ATGCAT	5 cut(s)	910, 976, 1081, 1213, 2668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.