Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0009766

Physical Location & Seq

Genomic Coordinates Reverse (-)

88273091 .. 88280720

Gene Structure 7630 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ54349

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3066 bp

ATGGCTTCTTCTACTGCGTGGGCGATGCTGCTGGTGGCGGTCTTATTGGCACAGTTGCTACCCGCAAGATCGGAGTACATAGCTTACAATACGACGGCGGGGATCGTCCCGGAGAAACTCAACGTTCATTTGGTTCCTCACTCTCACGACGACGTCGGGTGGCTTAAGACGGTGGACCAGTACTACGTGGGCGCCAACAATTCAATTCGAGGTGCTTGTGTACAGAACGTCATTGACTCTGTCATCTCTGCCTTATTGGAGGACAAGAATCGCAAGTTCATCTATGTTGAGATTGCATTCTTTCAGAGATGGTGGAGACAGCAAAGCCCTGCTATGAAGATCAAAGTCAAGGACCTGGTCACCTCCGGTCAACTCGAGTTCATAAATGGGGGCATGTGTATGCACGACGAGGCCACCCCGCATTACATTGACTTGATTGATCAGACTACCCTAGGGCATCAATTTATACTGAAAGAGTTTGGTCTGACTCCTCGAGTTGGCTGGCAGATTGATCCTTTTGGCCATTCTGCTGTTCAAGCTTACTTGCTTGGTGCTGAGTTAGGTTTCGATTCCCTCTTTTTCGCTCGAATTGATTACCAAGATAGAGCAGTCCGGCTCAGGGATAAGACTCTTGAGGTTATATGGCAGGGTTCTAAGTCCCTTGCTGCATCTTCACAGATTTTCACTGGCGTATTCCCCAGGCACTATGATCCTCCAGATGGTTTCGTATTTGAAATAAATGATGTGTCACCTCCTATTCAGGATGACATTCTCCTGTTTGACTACAATGTTCAAGAGCGAGTCAATGACTTTGTTGCAGCTGCTTTAGCTCAGGCTAATGTGACAAGAACAAATCATATTATGTGGACCATGGGGACAGATTTTCGCTACCAATATGGAAATTCATGGTTCAGGCAGATGGACAAATTCATTCATTATGTCAACAAGGATGGGCGTGTCAATGCATTTTATTCAACTCCATCAATCTACACTGATGCAAAATATGCAGCAGATGAGCAATGGCCTCTGAAAACTGATGATTTCTTTCCATATGCGGATCATCCAAATGCATATTGGACCGGTTATTTTACAAGTCGTCCGGCCTTTAAAGGTTATGTCCGAAGTTTGAGTGGTTACTATCTGGCAGCAAGGCAGTTGGAATTCTTCAAAGGCAGGAGTGATTCTGGAGCAAATACTGATGCATTAGCTGATGCTTTGGCTATTGTTCAACATCATGATGCAGTTAGTGGTACACAACGACAGCATGTTGCTGCTGATTATGCAATGCGATTATCAATAGGTTATTTGGAGGCTGAAAAGTTGGTCGCCTCTTCTCTTGCTTACCTTTCTGAGTCAGAACCAAGTTCTGGACAGAAGCGCATAGCCACAAAATTTGAACAGTGCCCTCTTCTAAACATCAGTTATTGTCCTCCATCAGAAACTGCTTTATCCGATGGGAAAAGCTTGGTTGTTGTTATCTACAATCCTCTTGGTTGGAAGAGAGAGGAAGTAATATGGATCCCTGTTTCCAATGAAAGGGTCACTGTTCAGGATTCTAGCCAAAGAAAAATTGATGCTCAGCTTTTACCTCTCTCAAATGCCACTTTGAAATTAAGAAGTTACTATGTCCGAGCATATTTGGGTATTACCCCAAGTGAACTGCCCAAATATTGGTTAGCTTTTTCAGTAACGGTACCACCACTTGGTTTCAGCTCTTACATTGTCTCAAGTGCAAATCAGACAGATAAAGATCGAAGCTCAACTATCTCCAAAGTATACACATCAGAAGGAAATACAAATAAGACCATACAAGTTGGGCAAGGGAGTTTAAAGCTACTTTATTCTGCAGAAGGAAAGCTTGCTCGTTATATTAACAGCAGAAATATGGTTACAGCGGTTGCTGAACAATCATATAGTTATTATACTGGAAACGATGGAACTGACAAAGATCCTCAGGCCTCTGGGGCATATGTATTTAGACCGAATGGCACAGTTGTTATAAAATCTGAAGAAAAGGTTTCTCTCACTGTGATGCGAGGCCCATTATTGGATGAAGTGCATCAGCAGATCAATCCATGGGTATCTCAGATTACAAGGTTATACAAAGGAAAGGAGCATGCTGAAGTAGAGTTCACTATTGGGCCTATACCCGTGGATGATGGTATTGGAAAAGAAATCACAACTCAACTTACAACAGCCATGAAGACCAATAAGACATTCTACACAGATTCCAATGGTCGCGACTTCATTAAGCGGATTCGAGATTTCAGGACAGATTGGGACCTACAAGTGAACCAACCAATTGCTGGAAATTATTACCCGATTAATCTTGGAATTTATGTGCAAGATAGCACTACAGAACTTTCAGTCTTGGTTGATCGTGCAGTGGGAGGATCTAGCTTAGTAGATGGTCAAGTAGAGCTGATGCTCCACAGGAGGCTAACTCATGATGATATCAGAGGTGTTGGCGAGATACTAAATGAAACGGTTTGCGTTTCTGAGAAGTGTGAAGGTTTAACTATCCAAGGGAAGTTTTATGTCAGAATCGATCCTGTTGGAGAAGGTTCTAAGTGGCGTCGCACAGCTGGCCAAGAGATAAGTGCTCCACTTTTATTGGCCTTCACCGAACAGGAAGGAAATGATTGGATGAATTCTCATGTATCAACATTTTCTGGAATTGACCCTTCCTATGCCTTACCAGACAATGTTGCTGTGATAACCCTCCAGGAACTTGAAAATGGGAAAGTACTGCTTCGTTTGGCTCACCTATACGAGACTGGCGAGGACAAGGACTATTCTGTATTGGCAAATGTGGAACTAAGAAAGTTGTTTCCAAGGAAGAAGATCAGCAAAGTGACAGAGATGAGTTTATCTGCCAATCAAGAAAGAGCTGAAATGGAGAAGAGGAGATTAGTTTGGAAAGTGGAAGGTTCTGCCACGGCCGAAGAGTCTAAGGTGGCGAGGGGAGGCGCTGTTGACCCTGCAAAACTGGTGGTGGAACTAGCACCAATGGAAATTCGCACTTTCCTTATTGACTTGGATTACCTCCATATGTATGGTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005774 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009505 GO:0009987 GO:0015923 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030312 GO:0031090 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1021

Amino Acids

114.69

Weight (kDa)

5.76

Isoelectric Point (pI)

34.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	41 - 352	1.3e-84	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	357 - 449	1.6e-19	Alpha mannosidase middle domain
Laman-like_dom	PF21260	524 - 575	8.4e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	607 - 820	3.5e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	902 - 1009	7e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26720 AT3G26720 AT3G26720 AT3G26720 AT3G26720
fragaria_vesca	FvH4_6g54280 FvH4_6g54280 FvH4_6g54280 FvH4_6g54280
malus_domestica	MD09G1002800.v1.1
prunus_persica	Prupe.3G313400_v2.0.a1 Prupe.3G313400_v2.0.a1
pyrus_communis	pycom111g00230
rosa_chinensis	RchiOBHm_Chr2g0176491
rosa_laevigata	RLG00000022417
rosa_roxburghii	Rroxscaffold_2G00076340
rosa_rugosa	Rorug02G0595100
rosa_samantha	Rh2AG673400 Rh2BG684700 Rh2DG698300
rosa_wichuraiana	Rw2G055230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2000
AatII	GACGTC	1 cut(s)	156
Acc65I	GGTACC	1 cut(s)	1693
AccB1I	GGYRCC	2 cut(s)	191, 1693
AccB7I	CCANNNNNTGG	4 cut(s)	1367, 1671, 1703, 2306
AccI	GTMKAC	1 cut(s)	1776
AccII	CGCG	1 cut(s)	2241
AciI	CCGC	7 cut(s)	38, 63, 98, 419, 1055, 1895, 2254
AclI	AACGTT	1 cut(s)	123
AclWI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2401, 2543
AcoI	YGGCCR	3 cut(s)	520, 2587, 2940
AcsI	RAATTY	7 cut(s)	901, 926, 1160, 1391, 2334, 2650, 3015
AcuI	CTGAAG	2 cut(s)	2028, 2142
AcyI	GRCGYC	3 cut(s)	153, 192, 2575
AfaI	GTAC	6 cut(s)	77, 182, 222, 1252, 1695, 2748
AfiI	CCNNNNNNNGG	9 cut(s)	497, 619, 719, 1367, 1536, 1671, 1703, 2047, 2306
AflII	CTTAAG	1 cut(s)	164
AgeI	ACCGGT	1 cut(s)	1079
AjnI	CCWGG	3 cut(s)	354, 698, 2724
AjuI	GAANNNNNNNTTGG	2 cut(s)	2201, 2233
Alw21I	GWGCWC	1 cut(s)	2605
Alw26I	GTCTC	3 cut(s)	310, 1729, 2768
AlwI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2401, 2543
AlwNI	CAGNNNCTG	1 cut(s)	1442
Ama87I	CYCGRG	2 cut(s)	374, 492
ApeKI	GCWGC	7 cut(s)	28, 665, 818, 821, 1007, 1145, 1271
ApoI	RAATTY	7 cut(s)	901, 926, 1160, 1391, 2334, 2650, 3015
AseI	ATTAAT	1 cut(s)	2325
AsiGI	ACCGGT	1 cut(s)	1079
Asp718I	GGTACC	1 cut(s)	1693
AspA2I	CCTAGG	1 cut(s)	451
AspLEI	GCGC	3 cut(s)	194, 1380, 2972
AspS9I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2039, 2141, 2281
AsuC2I	CCSGG	1 cut(s)	110
AsuHPI	GGTGA	4 cut(s)	352, 741, 2614, 2756
AvaI	CYCGRG	2 cut(s)	374, 492
AvaII	GGWCC	5 cut(s)	175, 352, 867, 1077, 2281
AvrII	CCTAGG	1 cut(s)	451
AxyI	CCTNAGG	1 cut(s)	1953
BaeGI	GKGCMC	1 cut(s)	1406
BalI	TGGCCA	2 cut(s)	522, 2589
BamHI	GGATCC	1 cut(s)	1518
BanI	GGYRCC	2 cut(s)	191, 1693
BbsI	GAAGAC	1 cut(s)	2210
Bbv12I	GWGCWC	1 cut(s)	2605
BbvI	GCAGC	7 cut(s)	15, 652, 808, 830, 1019, 1157, 1258
BceAI	ACGGC	2 cut(s)	111, 2955
BciT130I	CCWGG	3 cut(s)	356, 700, 2726
BclI	TGATCA	1 cut(s)	439
BcnI	CCSGG	1 cut(s)	110
BcoDI	GTCTC	3 cut(s)	310, 1729, 2768
BfaI	CTAG	4 cut(s)	452, 1557, 2397, 3002
BfmI	CTRYAG	2 cut(s)	1845, 2355
BfoI	RGCGCY	2 cut(s)	195, 2973
BfrI	CTTAAG	1 cut(s)	164
BglI	GCCNNNNNGGC	1 cut(s)	1964
BisI	GCNGC	7 cut(s)	29, 666, 819, 822, 1008, 1146, 1272
BlnI	CCTAGG	1 cut(s)	451
BlpI	GCTNAGC	1 cut(s)	1578
BlsI	GCNGC	7 cut(s)	30, 667, 820, 823, 1009, 1147, 1273
BmcAI	AGTACT	2 cut(s)	182, 2748
Bme1390I	CCNGG	4 cut(s)	110, 356, 700, 2726
Bme18I	GGWCC	5 cut(s)	175, 352, 867, 1077, 2281
BmeT110I	CYCGRG	2 cut(s)	374, 492
BmgT120I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2039, 2141, 2281
BmiI	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2282, 3061
BmrFI	CCNGG	4 cut(s)	110, 356, 700, 2726
BpiI	GAAGAC	1 cut(s)	2210
BplI	GAGNNNNNCTC	2 cut(s)	2428, 2460
BpmI	CTGGAG	3 cut(s)	699, 1206, 2708
Bpu10I	CCTNAGC	2 cut(s)	617, 831
Bpu1102I	GCTNAGC	1 cut(s)	1578
BpuEI	CTTGAG	2 cut(s)	653, 1711
BpuMI	CCSGG	1 cut(s)	110
Bsa29I	ATCGAT	1 cut(s)	2547
BsaAI	YACGTR	1 cut(s)	187
BsaBI	GATNNNNATC	1 cut(s)	1749
BsaHI	GRCGYC	3 cut(s)	153, 192, 2575
BsaJI	CCNNGG	8 cut(s)	451, 698, 870, 2075, 2151, 2524, 2834, 2937
BsaWI	WCCGGW	2 cut(s)	365, 1079
BsaXI	ACNNNNNCTCC	2 cut(s)	2958, 2988
Bsc4I	CCNNNNNNNGG	9 cut(s)	497, 619, 719, 1367, 1536, 1671, 1703, 2047, 2306
Bse118I	RCCGGY	1 cut(s)	1079
Bse1I	ACTGG	5 cut(s)	178, 691, 1930, 2782, 2994
Bse21I	CCTNAGG	1 cut(s)	1953
Bse3DI	GCAATG	2 cut(s)	1025, 1290
Bse8I	GATNNNNATC	1 cut(s)	1749
BseBI	CCWGG	3 cut(s)	356, 700, 2726
BseCI	ATCGAT	1 cut(s)	2547
BseDI	CCNNGG	8 cut(s)	451, 698, 870, 2075, 2151, 2524, 2834, 2937
BseGI	GGATG	6 cut(s)	769, 955, 1060, 2056, 2161, 2652
BseJI	GATNNNNATC	1 cut(s)	1749
BseLI	CCNNNNNNNGG	9 cut(s)	497, 619, 719, 1367, 1536, 1671, 1703, 2047, 2306
BseMI	GCAATG	2 cut(s)	1025, 1290
BseMII	CTCAG	8 cut(s)	546, 631, 845, 1341, 1592, 1967, 2099, 2490
BseNI	ACTGG	5 cut(s)	178, 691, 1930, 2782, 2994
BseRI	GAGGAG	2 cut(s)	480, 2920
BseSI	GKGCMC	1 cut(s)	1406
BseX3I	CGGCCG	1 cut(s)	2940
BseXI	GCAGC	7 cut(s)	15, 652, 808, 830, 1019, 1157, 1258
BsgI	GTGCAG	1 cut(s)	2403
Bsh1236I	CGCG	1 cut(s)	2241
Bsh1285I	CGRYCG	1 cut(s)	2943
BshNI	GGYRCC	2 cut(s)	191, 1693
BshTI	ACCGGT	1 cut(s)	1079
BshVI	ATCGAT	1 cut(s)	2547
BsiEI	CGRYCG	1 cut(s)	2943
BsiHKAI	GWGCWC	1 cut(s)	2605
BsiHKCI	CYCGRG	2 cut(s)	374, 492
BsiSI	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
BslFI	GGGAC	4 cut(s)	92, 643, 889, 2294
BslI	CCNNNNNNNGG	9 cut(s)	497, 619, 719, 1367, 1536, 1671, 1703, 2047, 2306
BsmAI	GTCTC	3 cut(s)	310, 1729, 2768
BsmFI	GGGAC	4 cut(s)	92, 643, 889, 2294
BsmI	GAATGC	1 cut(s)	296
BsoBI	CYCGRG	2 cut(s)	374, 492
Bsp1286I	GDGCHC	2 cut(s)	1406, 2605
Bsp1407I	TGTACA	1 cut(s)	220
Bsp1720I	GCTNAGC	1 cut(s)	1578
Bsp19I	CCATGG	2 cut(s)	870, 2075
Bsp68I	TCGCGA	1 cut(s)	2241
BspACI	CCGC	7 cut(s)	38, 63, 98, 419, 1055, 1895, 2254
BspCNI	CTCAG	8 cut(s)	547, 630, 844, 1342, 1591, 1966, 2098, 2491
BspDI	ATCGAT	1 cut(s)	2547
BspFNI	CGCG	1 cut(s)	2241
BspHI	TCATGA	2 cut(s)	1234, 2446
BspLI	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2282, 3061
BspMAI	CTGCAG	1 cut(s)	1849
BspPI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2401, 2543
BspT107I	GGYRCC	2 cut(s)	191, 1693
BspTI	CTTAAG	1 cut(s)	164
BsrDI	GCAATG	2 cut(s)	1025, 1290
BsrFI	RCCGGY	1 cut(s)	1079
BsrGI	TGTACA	1 cut(s)	220
BsrI	ACTGG	5 cut(s)	178, 691, 1930, 2782, 2994
BssAI	RCCGGY	1 cut(s)	1079
BssECI	CCNNGG	8 cut(s)	451, 698, 870, 2075, 2151, 2524, 2834, 2937
BssNAI	GTATAC	1 cut(s)	1777
BssNI	GRCGYC	3 cut(s)	153, 192, 2575
BssT1I	CCWWGG	5 cut(s)	451, 870, 2075, 2524, 2834
Bst1107I	GTATAC	1 cut(s)	1777
Bst2UI	CCWGG	3 cut(s)	356, 700, 2726
Bst4CI	ACNGT	8 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2029, 2488
Bst6I	CTCTTC	5 cut(s)	1336, 1413, 1493, 2897, 2940
BstACI	GRCGYC	3 cut(s)	153, 192, 2575
BstAFI	CTTAAG	1 cut(s)	164
BstAPI	GCANNNNNTGC	1 cut(s)	1004
BstAUI	TGTACA	1 cut(s)	220
BstBAI	YACGTR	1 cut(s)	187
BstC8I	GCNNGC	4 cut(s)	503, 1860, 2118, 2587
BstDSI	CCRYGG	4 cut(s)	870, 2075, 2151, 2937
BstEII	GGTNACC	1 cut(s)	358
BstF5I	GGATG	6 cut(s)	769, 955, 1060, 2056, 2161, 2652
BstFNI	CGCG	1 cut(s)	2241
BstH2I	RGCGCY	2 cut(s)	195, 2973
BstHHI	GCGC	3 cut(s)	194, 1380, 2972
BstMAI	GTCTC	3 cut(s)	310, 1729, 2768
BstMCI	CGRYCG	1 cut(s)	2943
BstMWI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2586
BstNI	CCWGG	3 cut(s)	356, 700, 2726
BstNSI	RCATGY	3 cut(s)	397, 1268, 2120
BstPI	GGTNACC	1 cut(s)	358
BstSCI	CCNGG	4 cut(s)	108, 354, 698, 2724
BstSFI	CTRYAG	2 cut(s)	1845, 2355
BstSLI	GKGCMC	1 cut(s)	1406
BstUI	CGCG	1 cut(s)	2241
BstV1I	GCAGC	7 cut(s)	15, 652, 808, 830, 1019, 1157, 1258
BstV2I	GAAGAC	1 cut(s)	2210
BstX2I	RGATCY	3 cut(s)	1518, 1948, 2393
BstXI	CCANNNNNNTGG	1 cut(s)	3056
BstYI	RGATCY	3 cut(s)	1518, 1948, 2393
BstZ17I	GTATAC	1 cut(s)	1777
BstZI	CGGCCG	1 cut(s)	2940
Bsu15I	ATCGAT	1 cut(s)	2547
Bsu36I	CCTNAGG	1 cut(s)	1953
BsuTUI	ATCGAT	1 cut(s)	2547
BtgI	CCRYGG	4 cut(s)	870, 2075, 2151, 2937
BtgZI	GCGATG	1 cut(s)	38
BtsCI	GGATG	6 cut(s)	769, 955, 1060, 2056, 2161, 2652
BtsI	GCAGTG	1 cut(s)	2391
BtsIMutI	CAGTG	6 cut(s)	684, 990, 1406, 1542, 2025, 2391
BtuMI	TCGCGA	1 cut(s)	2241
Cac8I	GCNNGC	4 cut(s)	503, 1860, 2118, 2587
CaiI	CAGNNNCTG	1 cut(s)	1442
CciI	TCATGA	2 cut(s)	1234, 2446
CfoI	GCGC	3 cut(s)	194, 1380, 2972
Cfr10I	RCCGGY	1 cut(s)	1079
Cfr13I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2039, 2141, 2281
ClaI	ATCGAT	1 cut(s)	2547
CseI	GACGC	1 cut(s)	2564
CsiI	ACCWGGT	1 cut(s)	354
Csp6I	GTAC	6 cut(s)	76, 181, 221, 1251, 1694, 2747
CspAI	ACCGGT	1 cut(s)	1079
CspCI	CAANNNNNGTGG	6 cut(s)	1375, 1410, 2595, 2630, 2973, 3008
CviQI	GTAC	6 cut(s)	76, 181, 221, 1251, 1694, 2747
DinI	GGCGCC	1 cut(s)	193
DraI	TTTAAA	2 cut(s)	1108, 1830
EaeI	YGGCCR	3 cut(s)	520, 2587, 2940
EagI	CGGCCG	1 cut(s)	2940
Eam1104I	CTCTTC	5 cut(s)	1336, 1413, 1493, 2897, 2940
EarI	CTCTTC	5 cut(s)	1336, 1413, 1493, 2897, 2940
EclXI	CGGCCG	1 cut(s)	2940
Eco130I	CCWWGG	5 cut(s)	451, 870, 2075, 2524, 2834
Eco147I	AGGCCT	1 cut(s)	1958
Eco32I	GATATC	1 cut(s)	2455
Eco47I	GGWCC	5 cut(s)	175, 352, 867, 1077, 2281
Eco52I	CGGCCG	1 cut(s)	2940
Eco57I	CTGAAG	2 cut(s)	2028, 2142
Eco81I	CCTNAGG	1 cut(s)	1953
Eco88I	CYCGRG	2 cut(s)	374, 492
Eco91I	GGTNACC	1 cut(s)	358
EcoO109I	RGGNCCY	2 cut(s)	352, 2281
EcoO65I	GGTNACC	1 cut(s)	358
EcoRI	GAATTC	2 cut(s)	1160, 2650
EcoRII	CCWGG	3 cut(s)	354, 698, 2724
EcoRV	GATATC	1 cut(s)	2455
EcoT14I	CCWWGG	5 cut(s)	451, 870, 2075, 2524, 2834
EcoT22I	ATGCAT	3 cut(s)	967, 1072, 1204
EgeI	GGCGCC	1 cut(s)	193
EheI	GGCGCC	1 cut(s)	193
ErhI	CCWWGG	5 cut(s)	451, 870, 2075, 2524, 2834
FalI	AAGNNNNNCTT	4 cut(s)	1842, 1874, 2736, 2768
FaqI	GGGAC	4 cut(s)	92, 643, 889, 2294
FauI	CCCGC	3 cut(s)	70, 91, 426
FauNDI	CATATG	3 cut(s)	1051, 1969, 3051
FbaI	TGATCA	1 cut(s)	439
FblI	GTMKAC	1 cut(s)	1776
Fnu4HI	GCNGC	7 cut(s)	29, 666, 819, 822, 1008, 1146, 1272
FokI	GGATG	6 cut(s)	776, 962, 1047, 2063, 2168, 2659
Fsp4HI	GCNGC	7 cut(s)	29, 666, 819, 822, 1008, 1146, 1272
FspBI	CTAG	4 cut(s)	452, 1557, 2397, 3002
GlaI	GCGC	3 cut(s)	193, 1379, 2971
GluI	GCNGC	7 cut(s)	29, 666, 819, 822, 1008, 1146, 1272
GsuI	CTGGAG	3 cut(s)	699, 1206, 2708
HaeII	RGCGCY	2 cut(s)	195, 2973
HapII	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
HgaI	GACGC	1 cut(s)	2564
HhaI	GCGC	3 cut(s)	194, 1380, 2972
Hin1I	GRCGYC	3 cut(s)	153, 192, 2575
Hin6I	GCGC	3 cut(s)	192, 1378, 2970
HinP1I	GCGC	3 cut(s)	192, 1378, 2970
HincII	GTYRAC	3 cut(s)	371, 943, 2977
HindII	GTYRAC	3 cut(s)	371, 943, 2977
HindIII	AAGCTT	3 cut(s)	537, 1462, 1856
HpaII	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
HphI	GGTGA	4 cut(s)	352, 741, 2614, 2756
Hpy99I	CGWCG	6 cut(s)	97, 152, 155, 158, 410, 2580
HpyAV	CCTTC	8 cut(s)	1781, 1844, 2504, 2555, 2627, 2629, 2694, 2921
HpyCH4III	ACNGT	8 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2029, 2488
HpyCH4IV	ACGT	4 cut(s)	123, 153, 186, 228
HpyF10VI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2586
HpySE526I	ACGT	4 cut(s)	123, 153, 186, 228
Hsp92I	GRCGYC	3 cut(s)	153, 192, 2575
HspAI	GCGC	3 cut(s)	192, 1378, 2970
KasI	GGCGCC	1 cut(s)	191
KpnI	GGTACC	1 cut(s)	1697
Ksp22I	TGATCA	1 cut(s)	439
LmnI	GCTCC	4 cut(s)	1187, 2113, 2433, 2608
Lsp1109I	GCAGC	7 cut(s)	15, 652, 808, 830, 1019, 1157, 1258
MabI	ACCWGGT	1 cut(s)	354
MaeI	CTAG	4 cut(s)	452, 1557, 2397, 3002
MaeII	ACGT	4 cut(s)	123, 153, 186, 228
MaeIII	GTNAC	9 cut(s)	358, 747, 841, 1133, 1540, 1619, 1687, 1888, 2854
MfeI	CAATTG	1 cut(s)	2301
MflI	RGATCY	3 cut(s)	1518, 1948, 2393
MhlI	GDGCHC	2 cut(s)	1406, 2605
MlsI	TGGCCA	2 cut(s)	522, 2589
MluNI	TGGCCA	2 cut(s)	522, 2589
Mly113I	GGCGCC	1 cut(s)	192
MlyI	GAGTC	6 cut(s)	230, 481, 622, 810, 1361, 2957
MmeI	TCCRAC	3 cut(s)	1137, 1475, 2536
Mox20I	TGGCCA	2 cut(s)	522, 2589
Mph1103I	ATGCAT	3 cut(s)	967, 1072, 1204
MscI	TGGCCA	2 cut(s)	522, 2589
MseI	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2250, 2325, 2516
MslI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2030, 3054
Msp20I	TGGCCA	2 cut(s)	522, 2589
MspA1I	CMGCKG	3 cut(s)	821, 1895, 2585
MspCI	CTTAAG	1 cut(s)	164
MspI	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
MspR9I	CCNGG	4 cut(s)	110, 356, 700, 2726
MunI	CAATTG	1 cut(s)	2301
Mva1269I	GAATGC	1 cut(s)	296
MvaI	CCWGG	3 cut(s)	356, 700, 2726
MvnI	CGCG	1 cut(s)	2241
MwoI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2586
NarI	GGCGCC	1 cut(s)	192
NciI	CCSGG	1 cut(s)	110
NcoI	CCATGG	2 cut(s)	870, 2075
NdeI	CATATG	3 cut(s)	1051, 1969, 3051
NlaIV	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2282, 3061
NmuCI	GTSAC	5 cut(s)	358, 747, 841, 1540, 2854
NruI	TCGCGA	1 cut(s)	2241
NsiI	ATGCAT	3 cut(s)	967, 1072, 1204
NspI	RCATGY	3 cut(s)	397, 1268, 2120
PaeI	GCATGC	1 cut(s)	2120
PaeR7I	CTCGAG	2 cut(s)	374, 492
PagI	TCATGA	2 cut(s)	1234, 2446
PceI	AGGCCT	1 cut(s)	1958
PcsI	WCGNNNNNNNCGW	1 cut(s)	2778
PctI	GAATGC	1 cut(s)	296
PfeI	GAWTC	7 cut(s)	268, 569, 1181, 1553, 2228, 2257, 2544
PflFI	GACNNNGTC	3 cut(s)	152, 239, 356
PflMI	CCANNNNNTGG	4 cut(s)	1367, 1671, 1703, 2306
PfoI	TCCNGGA	2 cut(s)	108, 2724
PinAI	ACCGGT	1 cut(s)	1079
PkrI	GCNGC	7 cut(s)	30, 667, 820, 823, 1009, 1147, 1273
PleI	GAGTC	6 cut(s)	230, 481, 622, 809, 1360, 2956
PluTI	GGCGCC	1 cut(s)	195
PpsI	GAGTC	6 cut(s)	230, 481, 622, 809, 1360, 2956
Ppu21I	YACGTR	1 cut(s)	187
PpuMI	RGGWCCY	2 cut(s)	352, 2281
PshBI	ATTAAT	1 cut(s)	2325
PsiI	TTATAA	1 cut(s)	2000
Psp1406I	AACGTT	1 cut(s)	123
Psp5II	RGGWCCY	2 cut(s)	352, 2281
Psp6I	CCWGG	3 cut(s)	354, 698, 2724
PspEI	GGTNACC	1 cut(s)	358
PspGI	CCWGG	3 cut(s)	354, 698, 2724
PspN4I	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2282, 3061
PspPI	GGNCC	7 cut(s)	175, 352, 867, 1077, 2039, 2141, 2281
PspPPI	RGGWCCY	2 cut(s)	352, 2281
PspXI	VCTCGAGB	2 cut(s)	374, 492
PstI	CTGCAG	1 cut(s)	1849
PstNI	CAGNNNCTG	1 cut(s)	1442
PsuI	RGATCY	3 cut(s)	1518, 1948, 2393
PsyI	GACNNNGTC	3 cut(s)	152, 239, 356
PvuII	CAGCTG	2 cut(s)	821, 2585
RruI	TCGCGA	1 cut(s)	2241
RsaI	GTAC	6 cut(s)	77, 182, 222, 1252, 1695, 2748
RsaNI	GTAC	6 cut(s)	76, 181, 221, 1251, 1694, 2747
RseI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2030, 3054
SaqAI	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2250, 2325, 2516
SatI	GCNGC	7 cut(s)	29, 666, 819, 822, 1008, 1146, 1272
Sau96I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2039, 2141, 2281
ScaI	AGTACT	2 cut(s)	182, 2748
SchI	GAGTC	6 cut(s)	230, 481, 622, 810, 1361, 2957
ScrFI	CCNGG	4 cut(s)	110, 356, 700, 2726
SduI	GDGCHC	2 cut(s)	1406, 2605
SexAI	ACCWGGT	1 cut(s)	354
SfcI	CTRYAG	2 cut(s)	1845, 2355
SfoI	GGCGCC	1 cut(s)	193
Sfr274I	CTCGAG	2 cut(s)	374, 492
SinI	GGWCC	5 cut(s)	175, 352, 867, 1077, 2281
SlaI	CTCGAG	2 cut(s)	374, 492
SmiMI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2030, 3054
SmlI	CTYRAG	5 cut(s)	164, 374, 492, 632, 1726
SmoI	CTYRAG	5 cut(s)	164, 374, 492, 632, 1726
SphI	GCATGC	1 cut(s)	2120
SseBI	AGGCCT	1 cut(s)	1958
SsiI	CCGC	7 cut(s)	38, 63, 98, 419, 1055, 1895, 2254
SspDI	GGCGCC	1 cut(s)	191
SspI	AATATT	1 cut(s)	1670
SspMI	CTAG	4 cut(s)	452, 1557, 2397, 3002
StuI	AGGCCT	1 cut(s)	1958
StyD4I	CCNGG	4 cut(s)	108, 354, 698, 2724
StyI	CCWWGG	5 cut(s)	451, 870, 2075, 2524, 2834
TaaI	ACNGT	8 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2029, 2488
TaiI	ACGT	4 cut(s)	126, 156, 189, 231
TaqI	TCGA	8 cut(s)	208, 375, 493, 567, 586, 1753, 2260, 2547
TaqII	GACCGA	1 cut(s)	1996
TatI	WGTACW	4 cut(s)	75, 180, 220, 2746
TfiI	GAWTC	7 cut(s)	268, 569, 1181, 1553, 2228, 2257, 2544
Tru1I	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2250, 2325, 2516
Tru9I	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2250, 2325, 2516
TscAI	CASTG	6 cut(s)	691, 997, 1406, 1549, 2032, 2391
TseFI	GTSAC	5 cut(s)	358, 747, 841, 1540, 2854
TseI	GCWGC	7 cut(s)	28, 665, 818, 821, 1007, 1145, 1271
Tsp45I	GTSAC	5 cut(s)	358, 747, 841, 1540, 2854
TspRI	CASTG	6 cut(s)	691, 997, 1406, 1549, 2032, 2391
Tth111I	GACNNNGTC	3 cut(s)	152, 239, 356
Van91I	CCANNNNNTGG	4 cut(s)	1367, 1671, 1703, 2306
Vha464I	CTTAAG	1 cut(s)	164
VpaK11BI	GGWCC	5 cut(s)	175, 352, 867, 1077, 2281
VspI	ATTAAT	1 cut(s)	2325
XapI	RAATTY	7 cut(s)	901, 926, 1160, 1391, 2334, 2650, 3015
XceI	RCATGY	3 cut(s)	397, 1268, 2120
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1071, 2303
XhoI	CTCGAG	2 cut(s)	374, 492
XmaJI	CCTAGG	1 cut(s)	451
XmiI	GTMKAC	1 cut(s)	1776
XspI	CTAG	4 cut(s)	452, 1557, 2397, 3002
ZraI	GACGTC	1 cut(s)	154
ZrmI	AGTACT	2 cut(s)	182, 2748
Zsp2I	ATGCAT	3 cut(s)	967, 1072, 1204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.