Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009766

Physical Location & Seq

Genomic Coordinates Forward (+)

218275 .. 233301

Gene Structure 15027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00076340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3078 bp

ATGGCTTCTTCTACTGCGTGGGCGATGCTGTTGGTGGCGGTGTTATTGGCACAGTTGCTGGCCGCCAGATCGGAGTACATAGCTTACAATACGACGGCGGGGATCGTCCCGGAGAAACTCAACGTTCATTTGGTTCCTCACTCTCACGACGACGTCGGCTGGCTTAAGACGGTGGACCAGTACTACGTGGGTGCCAACAATTCAATTCGAGGTGCTTGTGTGCAGAACGTCATTGACTCTGTCATCTCTGCCTTATTGGAGGACAAGAATCGCAAGTTCATCTATGTTGAGATTGCATTCTTTCAGAGATGGTGGAGACAGCAAAGCCCTGCTATGAAGATCAAAGTCAAGGACCTGGTCAGCTCCGGTCAACTTGAGCTCATAAATGGGGGCATGTGTATGCACGACGAGGCCACCCCGCATTACATTGACTTGATTGATCAGACTACCCTAGGGCATCAATTTATACTGAAAGAGTTTGGTCAGACTCCTCGAGTTGGCTGGCAGATTGATCCTTTTGGCCATTCTGCTGTTCAAGCTTACTTGCTTGGTGCGGAGTTAGGTTTCGATTCCCTCTTTTTTGCTCGAATTGATTACCAAGATAGAGCAGTCCGGCTCAGGGATAAGACTCTTGAGGTTATATGGCAGGGCTCTAAGTCCCTTGCTGCATCTTCACAGATTTTCACTGGCGTATTCCCCAGGCACTATGATCCTCCAGATGGTTTCGTATTTGAAATAAATGATGTGTCACCTCCTATTCAGGATGACATTCTCCTGTTTGACTACAATGTTCAAGAGCGAGTCAATGACTTTGTTGCAGCTGCTTTAGCTCAGGCTAATGTGACAAGAACAAATCATATTATGTGGACCATGGGAACAGATTTTCGCTACCAATATGGAAATTCATGGTTCAGGCAGATGGACAAATTCATTCATTATGTCAACCAGGATGGGCGTGTCAATGCATTTTATTCAACTCCATCAATCTACACTGATGCAAAATATGCAGCAGATGAGCAATGGCCTCTGAAAACTGATGATTTCTTTCCATATGCGGATCATCCAAATGCATATTGGACCGGTTATTTTACAAGTCGTCCGGCCTTTAAAGGTTACGTCCGAAGTTTGAGTGGTTACTATCTGGCAGCAAGGCAGTTGGAATTCTTCAAAGGCAGGAGTGATTCTGGAGCAAATACTGATGCATTAGCTGATGCTTTGGCTATTGTTCAACATCATGATGCAGTTAGTGGTACACAACGACAGCATGTTGCTGCTGATTATGCAATGCGATTATCAATAGGTTATTTGGAGGCTGAAAAGTTGGTCGCCTCTTCTCTTGCTTACCTTTCTGAGTCAGAACAAAGTTCTGGACAGAAGCGCATAGCCACAAAATTTGAACAGTGCCCTCTTCTAAACATCAGTTATTGTCCTCCATCAGAAACTGCTTTATCCGATGGGAAAAGCTTGGTTGTTGTTATCTACAATCCTCTTGGTTGGAAGAGAGAGGAAGTAATACGGATCCCTGTTTCCAATGAAAGGGTCACTGTTCAGGATTCTAGCCAAAGAAAAATTGATGCTCAGCTTTTACCTCTCTCAAATGCCACTTTGAAATTAAGAAGTTACTATGTCAGAGCATATTTGGGTATTACCCCAAGTGAACCACCCAAATATTGGCTAGCTTTTTCAGTAACGGTACCACCACTTGGTTTCAGCTCTTACATTGTCTCAAGTGCAAATCAGACAGATAAAGATCGAAGCTCAACTATCTCCAAAGTATACACATCAGAAGGAAATACAAATAAGACCATACAAGTTGGGCAAGGGAGTTTAAAGCTACTTTATTCTGCAGAAGGAAAGCTTGCTCGTTATATTAACAGCAGAAATATGGTTACAGCGGTTGCTGAACAATCATATAGTTATTATACTGGAAACGATGGAACTGACAAAGATCCTCAGGCCTCTGGGGCATATGTATTTAGACCAAATGGCACAGTTGTTATAAAATCTGAAGAAAAGTATCTTTATCAGGTTTCTCTCACCATGGTGCGAGGCCCATTATTGGATGAAGTGCATCAGCAGATCAATCCATGGGTATCTCAGATTACAAGGTTATACAAAGGAAAGGAGCATGCTGAAGTAGAGTTCACTATTGGGCCTATACCCGTGGATGATGGTATTGGAAAAGAAATCACAACTCAACTTACAACAGCCATGAAGACCAATAAGACATTCTACACAGATTCCAATGGTCGTGACTTCATTAAGCGGATTCGAGATTTCAGGACAGATTGGGACCTACAAGTGAACCAACCAATTGCTGGAAATTATTACCCGATTAATCTTGGAATTTATGTGCAAGATAGCACTACAGAACTTTCAGTCTTGGTTGATCGTGCAGTGGGAGGATCTAGCTTAGTAGATGGTCAAGTAGAGCTGATGCTCCACAGGAGGCTAACTCATGATGATATCAGAGGTGTTGGCGAGATACTAAATGAAACGGTTTGCGTTTCTGGGAAGTGTGAAGGTTTAACTATCCAAGGGAAGTTTTATGTCAGAATCGATCCTGTTGGAGAAGGTTCTAAGTGGCGTCGCACAGCTGGCCAAGAGATAAGTGCTCCACTTTTATTGGCCTTTGCCGAACAGGAAGGAAATGATTGGATGAATTCTCATGTATCAACATTTTCTGGAATTGACCCTTCCTATGCCTTACCAGACAATGTTGCTGTGATAACCCTCCAGGAACTTGAAAATGGGAAAGTACTGCTTCGTTTGGCTCACCTATACGAGACTGACGAGGACAAGGACTATTCTGTATTGGCAAATGTGGAACTAAGAAAGTTGTTTCCAAGGAAGAAGATCAGCAAAGTGACAGAGATGAGTTTATCTGCCAATCAAGAAAGAGCTGAAATGGAGAAGAGGAAATTAGTTTGGAAAGTGGAAGGTTCTGCCGCGGCCGAAGAGTCGAAGGTGGTGAGGGGAGGTGCTGTTGACCCTGCAAAACTGGTGGTGGAACTAGCACCAATGGAAATACGCACTTTCCTTATTGACTTGGATTACCTCCATATGTATGGTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005774 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009505 GO:0009987 GO:0015923 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030312 GO:0031090 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1025

Amino Acids

115.11

Weight (kDa)

5.76

Isoelectric Point (pI)

33.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	41 - 352	1.8e-84	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	357 - 449	1.6e-19	Alpha mannosidase middle domain
Laman-like_dom	PF21260	524 - 575	7.5e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	607 - 824	1.7e-44	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	906 - 1013	6.1e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26720 AT3G26720 AT3G26720 AT3G26720 AT3G26720
fragaria_vesca	FvH4_6g54280 FvH4_6g54280 FvH4_6g54280 FvH4_6g54280
malus_domestica	MD09G1002800.v1.1
prunus_persica	Prupe.3G313400_v2.0.a1 Prupe.3G313400_v2.0.a1
pyrus_communis	pycom111g00230
rosa_chinensis	RchiOBHm_Chr2g0176491
rosa_laevigata	RLG00000022417
rosa_roxburghii	Rroxscaffold_2G00076340
rosa_rugosa	Rorug02G0595100
rosa_samantha	Rh2AG673400 Rh2BG684700 Rh2DG698300
rosa_wichuraiana	Rw2G055230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2000
AatII	GACGTC	1 cut(s)	156
Acc65I	GGTACC	1 cut(s)	1693
AccB1I	GGYRCC	2 cut(s)	191, 1693
AccB7I	CCANNNNNTGG	3 cut(s)	1671, 1703, 2318
AccI	GTMKAC	1 cut(s)	1776
AccII	CGCG	1 cut(s)	2951
AclI	AACGTT	1 cut(s)	123
AclWI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2413, 2555
AcoI	YGGCCR	4 cut(s)	60, 520, 2599, 2952
AcsI	RAATTY	6 cut(s)	901, 926, 1160, 1391, 2346, 2662
AcuI	CTGAAG	2 cut(s)	2028, 2154
AcyI	GRCGYC	2 cut(s)	153, 2587
AfaI	GTAC	5 cut(s)	77, 182, 1252, 1695, 2760
AfiI	CCNNNNNNNGG	8 cut(s)	497, 619, 719, 1536, 1671, 1703, 2059, 2318
AflII	CTTAAG	1 cut(s)	164
AgeI	ACCGGT	1 cut(s)	1079
AjnI	CCWGG	4 cut(s)	354, 698, 945, 2736
AjuI	GAANNNNNNNTTGG	2 cut(s)	2213, 2245
AleI	CACNNNNGTG	1 cut(s)	2042
Alw21I	GWGCWC	2 cut(s)	381, 2617
Alw26I	GTCTC	3 cut(s)	310, 1729, 2780
AlwI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2413, 2555
AlwNI	CAGNNNCTG	2 cut(s)	58, 1442
Ama87I	CYCGRG	1 cut(s)	492
ApeKI	GCWGC	6 cut(s)	665, 818, 821, 1007, 1145, 1271
ApoI	RAATTY	6 cut(s)	901, 926, 1160, 1391, 2346, 2662
AseI	ATTAAT	1 cut(s)	2337
AsiGI	ACCGGT	1 cut(s)	1079
Asp718I	GGTACC	1 cut(s)	1693
AspA2I	CCTAGG	1 cut(s)	451
AspLEI	GCGC	1 cut(s)	1380
AspS9I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2051, 2153, 2293
AsuC2I	CCSGG	1 cut(s)	110
AsuHPI	GGTGA	4 cut(s)	741, 2029, 2768, 2983
AsuNHI	GCTAGC	1 cut(s)	1675
AvaI	CYCGRG	1 cut(s)	492
AvaII	GGWCC	5 cut(s)	175, 352, 867, 1077, 2293
AvrII	CCTAGG	1 cut(s)	451
AxyI	CCTNAGG	1 cut(s)	1953
BaeGI	GKGCMC	1 cut(s)	1406
BalI	TGGCCA	2 cut(s)	522, 2601
BamHI	GGATCC	1 cut(s)	1518
BanI	GGYRCC	2 cut(s)	191, 1693
BanII	GRGCYC	2 cut(s)	381, 653
BbsI	GAAGAC	1 cut(s)	2222
Bbv12I	GWGCWC	2 cut(s)	381, 2617
BbvI	GCAGC	6 cut(s)	652, 808, 830, 1019, 1157, 1258
BceAI	ACGGC	1 cut(s)	111
BciT130I	CCWGG	4 cut(s)	356, 700, 947, 2738
BclI	TGATCA	1 cut(s)	439
BcnI	CCSGG	1 cut(s)	110
BcoDI	GTCTC	3 cut(s)	310, 1729, 2780
BfaI	CTAG	5 cut(s)	452, 1557, 1676, 2409, 3014
BfmI	CTRYAG	2 cut(s)	1845, 2367
BfrI	CTTAAG	1 cut(s)	164
BglI	GCCNNNNNGGC	1 cut(s)	1964
BisI	GCNGC	9 cut(s)	63, 666, 819, 822, 1008, 1146, 1272, 2949, 2952
BlnI	CCTAGG	1 cut(s)	451
BlpI	GCTNAGC	1 cut(s)	1578
BlsI	GCNGC	9 cut(s)	64, 667, 820, 823, 1009, 1147, 1273, 2950, 2953
BmcAI	AGTACT	2 cut(s)	182, 2760
Bme1390I	CCNGG	5 cut(s)	110, 356, 700, 947, 2738
Bme18I	GGWCC	5 cut(s)	175, 352, 867, 1077, 2293
BmeT110I	CYCGRG	1 cut(s)	492
BmgT120I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2051, 2153, 2293
BmiI	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2294, 3073
BmrFI	CCNGG	5 cut(s)	110, 356, 700, 947, 2738
BmtI	GCTAGC	1 cut(s)	1679
BpiI	GAAGAC	1 cut(s)	2222
BplI	GAGNNNNNCTC	2 cut(s)	2440, 2472
BpmI	CTGGAG	3 cut(s)	699, 1206, 2720
Bpu10I	CCTNAGC	2 cut(s)	617, 831
Bpu1102I	GCTNAGC	1 cut(s)	1578
BpuEI	CTTGAG	3 cut(s)	395, 653, 1711
BpuMI	CCSGG	1 cut(s)	110
Bsa29I	ATCGAT	1 cut(s)	2559
BsaAI	YACGTR	1 cut(s)	187
BsaBI	GATNNNNATC	1 cut(s)	1749
BsaHI	GRCGYC	2 cut(s)	153, 2587
BsaJI	CCNNGG	9 cut(s)	451, 698, 870, 2040, 2087, 2163, 2536, 2846, 2949
BsaWI	WCCGGW	2 cut(s)	365, 1079
BsaXI	ACNNNNNCTCC	2 cut(s)	2970, 3000
Bsc4I	CCNNNNNNNGG	8 cut(s)	497, 619, 719, 1536, 1671, 1703, 2059, 2318
Bse118I	RCCGGY	1 cut(s)	1079
Bse1I	ACTGG	4 cut(s)	178, 691, 1930, 3006
Bse21I	CCTNAGG	1 cut(s)	1953
Bse3DI	GCAATG	2 cut(s)	1025, 1290
Bse8I	GATNNNNATC	1 cut(s)	1749
BseBI	CCWGG	4 cut(s)	356, 700, 947, 2738
BseCI	ATCGAT	1 cut(s)	2559
BseDI	CCNNGG	9 cut(s)	451, 698, 870, 2040, 2087, 2163, 2536, 2846, 2949
BseGI	GGATG	6 cut(s)	769, 955, 1060, 2068, 2173, 2664
BseJI	GATNNNNATC	1 cut(s)	1749
BseLI	CCNNNNNNNGG	8 cut(s)	497, 619, 719, 1536, 1671, 1703, 2059, 2318
BseMI	GCAATG	2 cut(s)	1025, 1290
BseMII	CTCAG	6 cut(s)	631, 845, 1341, 1592, 1967, 2111
BseNI	ACTGG	4 cut(s)	178, 691, 1930, 3006
BseRI	GAGGAG	1 cut(s)	480
BseSI	GKGCMC	1 cut(s)	1406
BseX3I	CGGCCG	1 cut(s)	2952
BseXI	GCAGC	6 cut(s)	652, 808, 830, 1019, 1157, 1258
BsgI	GTGCAG	2 cut(s)	242, 2415
Bsh1236I	CGCG	1 cut(s)	2951
Bsh1285I	CGRYCG	1 cut(s)	2955
BshNI	GGYRCC	2 cut(s)	191, 1693
BshTI	ACCGGT	1 cut(s)	1079
BshVI	ATCGAT	1 cut(s)	2559
BsiEI	CGRYCG	1 cut(s)	2955
BsiHKAI	GWGCWC	2 cut(s)	381, 2617
BsiHKCI	CYCGRG	1 cut(s)	492
BsiSI	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
BslFI	GGGAC	3 cut(s)	92, 643, 2306
BslI	CCNNNNNNNGG	8 cut(s)	497, 619, 719, 1536, 1671, 1703, 2059, 2318
BsmAI	GTCTC	3 cut(s)	310, 1729, 2780
BsmFI	GGGAC	3 cut(s)	92, 643, 2306
BsmI	GAATGC	1 cut(s)	296
BsoBI	CYCGRG	1 cut(s)	492
Bsp1286I	GDGCHC	4 cut(s)	381, 653, 1406, 2617
Bsp1720I	GCTNAGC	1 cut(s)	1578
Bsp19I	CCATGG	3 cut(s)	870, 2040, 2087
BspCNI	CTCAG	6 cut(s)	630, 844, 1342, 1591, 1966, 2110
BspDI	ATCGAT	1 cut(s)	2559
BspFNI	CGCG	1 cut(s)	2951
BspHI	TCATGA	2 cut(s)	1234, 2458
BspLI	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2294, 3073
BspMAI	CTGCAG	1 cut(s)	1849
BspOI	GCTAGC	1 cut(s)	1679
BspPI	GGATC	9 cut(s)	110, 506, 704, 1065, 1513, 1526, 1943, 2413, 2555
BspT107I	GGYRCC	2 cut(s)	191, 1693
BspTI	CTTAAG	1 cut(s)	164
BsrDI	GCAATG	2 cut(s)	1025, 1290
BsrFI	RCCGGY	1 cut(s)	1079
BsrI	ACTGG	4 cut(s)	178, 691, 1930, 3006
BssAI	RCCGGY	1 cut(s)	1079
BssECI	CCNNGG	9 cut(s)	451, 698, 870, 2040, 2087, 2163, 2536, 2846, 2949
BssNAI	GTATAC	1 cut(s)	1777
BssNI	GRCGYC	2 cut(s)	153, 2587
BssT1I	CCWWGG	6 cut(s)	451, 870, 2040, 2087, 2536, 2846
Bst1107I	GTATAC	1 cut(s)	1777
Bst2UI	CCWGG	4 cut(s)	356, 700, 947, 2738
Bst4CI	ACNGT	7 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2500
Bst6I	CTCTTC	5 cut(s)	1336, 1413, 1493, 2909, 2952
BstACI	GRCGYC	2 cut(s)	153, 2587
BstAFI	CTTAAG	1 cut(s)	164
BstAPI	GCANNNNNTGC	1 cut(s)	1004
BstBAI	YACGTR	1 cut(s)	187
BstC8I	GCNNGC	7 cut(s)	60, 161, 503, 1677, 1860, 2130, 2599
BstDSI	CCRYGG	5 cut(s)	870, 2040, 2087, 2163, 2949
BstF5I	GGATG	6 cut(s)	769, 955, 1060, 2068, 2173, 2664
BstFNI	CGCG	1 cut(s)	2951
BstHHI	GCGC	1 cut(s)	1380
BstMAI	GTCTC	3 cut(s)	310, 1729, 2780
BstMCI	CGRYCG	1 cut(s)	2955
BstMWI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2598
BstNI	CCWGG	4 cut(s)	356, 700, 947, 2738
BstNSI	RCATGY	3 cut(s)	397, 1268, 2132
BstSCI	CCNGG	5 cut(s)	108, 354, 698, 945, 2736
BstSFI	CTRYAG	2 cut(s)	1845, 2367
BstSLI	GKGCMC	1 cut(s)	1406
BstUI	CGCG	1 cut(s)	2951
BstV1I	GCAGC	6 cut(s)	652, 808, 830, 1019, 1157, 1258
BstV2I	GAAGAC	1 cut(s)	2222
BstX2I	RGATCY	3 cut(s)	1518, 1948, 2405
BstXI	CCANNNNNNTGG	1 cut(s)	3068
BstYI	RGATCY	3 cut(s)	1518, 1948, 2405
BstZ17I	GTATAC	1 cut(s)	1777
BstZI	CGGCCG	1 cut(s)	2952
Bsu15I	ATCGAT	1 cut(s)	2559
Bsu36I	CCTNAGG	1 cut(s)	1953
BsuTUI	ATCGAT	1 cut(s)	2559
BtgI	CCRYGG	5 cut(s)	870, 2040, 2087, 2163, 2949
BtgZI	GCGATG	1 cut(s)	38
BtsCI	GGATG	6 cut(s)	769, 955, 1060, 2068, 2173, 2664
BtsI	GCAGTG	1 cut(s)	2403
BtsIMutI	CAGTG	5 cut(s)	684, 990, 1406, 1542, 2403
Cac8I	GCNNGC	7 cut(s)	60, 161, 503, 1677, 1860, 2130, 2599
CaiI	CAGNNNCTG	2 cut(s)	58, 1442
CciI	TCATGA	2 cut(s)	1234, 2458
CfoI	GCGC	1 cut(s)	1380
Cfr10I	RCCGGY	1 cut(s)	1079
Cfr13I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2051, 2153, 2293
Cfr42I	CCGCGG	1 cut(s)	2952
ClaI	ATCGAT	1 cut(s)	2559
CseI	GACGC	1 cut(s)	2576
CsiI	ACCWGGT	1 cut(s)	354
Csp6I	GTAC	5 cut(s)	76, 181, 1251, 1694, 2759
CspAI	ACCGGT	1 cut(s)	1079
CspCI	CAANNNNNGTGG	6 cut(s)	1375, 1410, 2607, 2642, 2985, 3020
CviQI	GTAC	5 cut(s)	76, 181, 1251, 1694, 2759
DraI	TTTAAA	2 cut(s)	1108, 1830
EaeI	YGGCCR	4 cut(s)	60, 520, 2599, 2952
EagI	CGGCCG	1 cut(s)	2952
Eam1104I	CTCTTC	5 cut(s)	1336, 1413, 1493, 2909, 2952
EarI	CTCTTC	5 cut(s)	1336, 1413, 1493, 2909, 2952
Ecl136II	GAGCTC	1 cut(s)	379
EclXI	CGGCCG	1 cut(s)	2952
Eco130I	CCWWGG	6 cut(s)	451, 870, 2040, 2087, 2536, 2846
Eco147I	AGGCCT	1 cut(s)	1958
Eco24I	GRGCYC	2 cut(s)	381, 653
Eco32I	GATATC	1 cut(s)	2467
Eco47I	GGWCC	5 cut(s)	175, 352, 867, 1077, 2293
Eco52I	CGGCCG	1 cut(s)	2952
Eco53kI	GAGCTC	1 cut(s)	379
Eco57I	CTGAAG	2 cut(s)	2028, 2154
Eco81I	CCTNAGG	1 cut(s)	1953
Eco88I	CYCGRG	1 cut(s)	492
EcoICRI	GAGCTC	1 cut(s)	379
EcoO109I	RGGNCCY	2 cut(s)	352, 2293
EcoRI	GAATTC	2 cut(s)	1160, 2662
EcoRII	CCWGG	4 cut(s)	354, 698, 945, 2736
EcoRV	GATATC	1 cut(s)	2467
EcoT14I	CCWWGG	6 cut(s)	451, 870, 2040, 2087, 2536, 2846
EcoT22I	ATGCAT	3 cut(s)	967, 1072, 1204
EcoT38I	GRGCYC	2 cut(s)	381, 653
ErhI	CCWWGG	6 cut(s)	451, 870, 2040, 2087, 2536, 2846
FalI	AAGNNNNNCTT	4 cut(s)	1842, 1874, 2748, 2780
FaqI	GGGAC	3 cut(s)	92, 643, 2306
FauI	CCCGC	2 cut(s)	91, 426
FauNDI	CATATG	3 cut(s)	1051, 1969, 3063
FbaI	TGATCA	1 cut(s)	439
FblI	GTMKAC	1 cut(s)	1776
Fnu4HI	GCNGC	9 cut(s)	63, 666, 819, 822, 1008, 1146, 1272, 2949, 2952
FokI	GGATG	6 cut(s)	776, 962, 1047, 2075, 2180, 2671
FriOI	GRGCYC	2 cut(s)	381, 653
Fsp4HI	GCNGC	9 cut(s)	63, 666, 819, 822, 1008, 1146, 1272, 2949, 2952
FspBI	CTAG	5 cut(s)	452, 1557, 1676, 2409, 3014
GlaI	GCGC	1 cut(s)	1379
GluI	GCNGC	9 cut(s)	63, 666, 819, 822, 1008, 1146, 1272, 2949, 2952
GsuI	CTGGAG	3 cut(s)	699, 1206, 2720
HapII	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
HgaI	GACGC	1 cut(s)	2576
HhaI	GCGC	1 cut(s)	1380
Hin1I	GRCGYC	2 cut(s)	153, 2587
Hin6I	GCGC	1 cut(s)	1378
HinP1I	GCGC	1 cut(s)	1378
HincII	GTYRAC	3 cut(s)	371, 943, 2989
HindII	GTYRAC	3 cut(s)	371, 943, 2989
HindIII	AAGCTT	3 cut(s)	537, 1462, 1856
HpaII	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
HphI	GGTGA	4 cut(s)	741, 2029, 2768, 2983
Hpy99I	CGWCG	6 cut(s)	97, 152, 155, 158, 410, 2592
HpyAV	CCTTC	8 cut(s)	1781, 1844, 2516, 2567, 2639, 2706, 2933, 2959
HpyCH4III	ACNGT	7 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2500
HpyCH4IV	ACGT	5 cut(s)	123, 153, 186, 228, 1116
HpyF10VI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2598
HpySE526I	ACGT	5 cut(s)	123, 153, 186, 228, 1116
Hsp92I	GRCGYC	2 cut(s)	153, 2587
HspAI	GCGC	1 cut(s)	1378
KpnI	GGTACC	1 cut(s)	1697
Ksp22I	TGATCA	1 cut(s)	439
KspI	CCGCGG	1 cut(s)	2952
LmnI	GCTCC	5 cut(s)	368, 1187, 2125, 2445, 2620
Lsp1109I	GCAGC	6 cut(s)	652, 808, 830, 1019, 1157, 1258
MabI	ACCWGGT	1 cut(s)	354
MaeI	CTAG	5 cut(s)	452, 1557, 1676, 2409, 3014
MaeII	ACGT	5 cut(s)	123, 153, 186, 228, 1116
MfeI	CAATTG	1 cut(s)	2313
MflI	RGATCY	3 cut(s)	1518, 1948, 2405
MhlI	GDGCHC	4 cut(s)	381, 653, 1406, 2617
MlsI	TGGCCA	2 cut(s)	522, 2601
MluNI	TGGCCA	2 cut(s)	522, 2601
MlyI	GAGTC	6 cut(s)	230, 481, 622, 810, 1361, 2969
MmeI	TCCRAC	3 cut(s)	1137, 1475, 2548
Mox20I	TGGCCA	2 cut(s)	522, 2601
Mph1103I	ATGCAT	3 cut(s)	967, 1072, 1204
MscI	TGGCCA	2 cut(s)	522, 2601
MseI	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2262, 2337, 2528
MslI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2042, 3066
Msp20I	TGGCCA	2 cut(s)	522, 2601
MspA1I	CMGCKG	4 cut(s)	821, 1895, 2597, 2951
MspCI	CTTAAG	1 cut(s)	164
MspI	CCGG	5 cut(s)	110, 366, 613, 1080, 1100
MspR9I	CCNGG	5 cut(s)	110, 356, 700, 947, 2738
MunI	CAATTG	1 cut(s)	2313
Mva1269I	GAATGC	1 cut(s)	296
MvaI	CCWGG	4 cut(s)	356, 700, 947, 2738
MvnI	CGCG	1 cut(s)	2951
MwoI	GCNNNNNNNGC	6 cut(s)	536, 827, 1004, 1280, 1964, 2598
NciI	CCSGG	1 cut(s)	110
NcoI	CCATGG	3 cut(s)	870, 2040, 2087
NdeI	CATATG	3 cut(s)	1051, 1969, 3063
NheI	GCTAGC	1 cut(s)	1675
NlaIV	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2294, 3073
NmuCI	GTSAC	5 cut(s)	747, 841, 1540, 2252, 2866
NsiI	ATGCAT	3 cut(s)	967, 1072, 1204
NspI	RCATGY	3 cut(s)	397, 1268, 2132
OliI	CACNNNNGTG	1 cut(s)	2042
PaeI	GCATGC	1 cut(s)	2132
PaeR7I	CTCGAG	1 cut(s)	492
PagI	TCATGA	2 cut(s)	1234, 2458
PceI	AGGCCT	1 cut(s)	1958
PcsI	WCGNNNNNNNCGW	1 cut(s)	2790
PctI	GAATGC	1 cut(s)	296
PfeI	GAWTC	7 cut(s)	268, 569, 1181, 1553, 2240, 2269, 2556
PflFI	GACNNNGTC	3 cut(s)	152, 239, 356
PflMI	CCANNNNNTGG	3 cut(s)	1671, 1703, 2318
PfoI	TCCNGGA	2 cut(s)	108, 2736
PinAI	ACCGGT	1 cut(s)	1079
PkrI	GCNGC	9 cut(s)	64, 667, 820, 823, 1009, 1147, 1273, 2950, 2953
PleI	GAGTC	6 cut(s)	230, 481, 622, 809, 1360, 2968
PpsI	GAGTC	6 cut(s)	230, 481, 622, 809, 1360, 2968
Ppu21I	YACGTR	1 cut(s)	187
PpuMI	RGGWCCY	2 cut(s)	352, 2293
PshBI	ATTAAT	1 cut(s)	2337
PsiI	TTATAA	1 cut(s)	2000
Psp124BI	GAGCTC	1 cut(s)	381
Psp1406I	AACGTT	1 cut(s)	123
Psp5II	RGGWCCY	2 cut(s)	352, 2293
Psp6I	CCWGG	4 cut(s)	354, 698, 945, 2736
PspGI	CCWGG	4 cut(s)	354, 698, 945, 2736
PspN4I	GGNNCC	6 cut(s)	135, 193, 1520, 1695, 2294, 3073
PspPI	GGNCC	7 cut(s)	175, 352, 867, 1077, 2051, 2153, 2293
PspPPI	RGGWCCY	2 cut(s)	352, 2293
PspXI	VCTCGAGB	1 cut(s)	492
PstI	CTGCAG	1 cut(s)	1849
PstNI	CAGNNNCTG	2 cut(s)	58, 1442
PsuI	RGATCY	3 cut(s)	1518, 1948, 2405
PsyI	GACNNNGTC	3 cut(s)	152, 239, 356
PvuII	CAGCTG	2 cut(s)	821, 2597
RsaI	GTAC	5 cut(s)	77, 182, 1252, 1695, 2760
RsaNI	GTAC	5 cut(s)	76, 181, 1251, 1694, 2759
RseI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2042, 3066
SacI	GAGCTC	1 cut(s)	381
SacII	CCGCGG	1 cut(s)	2952
SaqAI	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2262, 2337, 2528
SatI	GCNGC	9 cut(s)	63, 666, 819, 822, 1008, 1146, 1272, 2949, 2952
Sau96I	GGNCC	7 cut(s)	175, 352, 867, 1077, 2051, 2153, 2293
ScaI	AGTACT	2 cut(s)	182, 2760
SchI	GAGTC	6 cut(s)	230, 481, 622, 810, 1361, 2969
ScrFI	CCNGG	5 cut(s)	110, 356, 700, 947, 2738
SduI	GDGCHC	4 cut(s)	381, 653, 1406, 2617
SexAI	ACCWGGT	1 cut(s)	354
SfcI	CTRYAG	2 cut(s)	1845, 2367
Sfr274I	CTCGAG	1 cut(s)	492
Sfr303I	CCGCGG	1 cut(s)	2952
SgrBI	CCGCGG	1 cut(s)	2952
SinI	GGWCC	5 cut(s)	175, 352, 867, 1077, 2293
SlaI	CTCGAG	1 cut(s)	492
SmiMI	CAYNNNNRTG	5 cut(s)	398, 1065, 1236, 2042, 3066
SmlI	CTYRAG	5 cut(s)	164, 374, 492, 632, 1726
SmoI	CTYRAG	5 cut(s)	164, 374, 492, 632, 1726
SphI	GCATGC	1 cut(s)	2132
SseBI	AGGCCT	1 cut(s)	1958
SspI	AATATT	1 cut(s)	1670
SspMI	CTAG	5 cut(s)	452, 1557, 1676, 2409, 3014
SstI	GAGCTC	1 cut(s)	381
StuI	AGGCCT	1 cut(s)	1958
StyD4I	CCNGG	5 cut(s)	108, 354, 698, 945, 2736
StyI	CCWWGG	6 cut(s)	451, 870, 2040, 2087, 2536, 2846
TaaI	ACNGT	7 cut(s)	54, 172, 1401, 1546, 1693, 1993, 2500
TaiI	ACGT	5 cut(s)	126, 156, 189, 231, 1119
TaqI	TCGA	8 cut(s)	208, 493, 567, 586, 1753, 2272, 2559, 2963
TatI	WGTACW	3 cut(s)	75, 180, 2758
TauI	GCSGC	3 cut(s)	65, 2951, 2954
TfiI	GAWTC	7 cut(s)	268, 569, 1181, 1553, 2240, 2269, 2556
Tru1I	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2262, 2337, 2528
Tru9I	TTAA	8 cut(s)	165, 1107, 1613, 1829, 1872, 2262, 2337, 2528
TscAI	CASTG	5 cut(s)	691, 997, 1406, 1549, 2403
TseFI	GTSAC	5 cut(s)	747, 841, 1540, 2252, 2866
TseI	GCWGC	6 cut(s)	665, 818, 821, 1007, 1145, 1271
Tsp45I	GTSAC	5 cut(s)	747, 841, 1540, 2252, 2866
TspGWI	ACGGA	1 cut(s)	1531
TspRI	CASTG	5 cut(s)	691, 997, 1406, 1549, 2403
Tth111I	GACNNNGTC	3 cut(s)	152, 239, 356
Van91I	CCANNNNNTGG	3 cut(s)	1671, 1703, 2318
Vha464I	CTTAAG	1 cut(s)	164
VpaK11BI	GGWCC	5 cut(s)	175, 352, 867, 1077, 2293
VspI	ATTAAT	1 cut(s)	2337
XapI	RAATTY	6 cut(s)	901, 926, 1160, 1391, 2346, 2662
XceI	RCATGY	3 cut(s)	397, 1268, 2132
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1071, 1668, 2315
XhoI	CTCGAG	1 cut(s)	492
XmaJI	CCTAGG	1 cut(s)	451
XmiI	GTMKAC	1 cut(s)	1776
XspI	CTAG	5 cut(s)	452, 1557, 1676, 2409, 3014
ZraI	GACGTC	1 cut(s)	154
ZrmI	AGTACT	2 cut(s)	182, 2760
Zsp2I	ATGCAT	3 cut(s)	967, 1072, 1204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.