Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009766

Physical Location & Seq

Genomic Coordinates Reverse (-)

82691290 .. 82697865

Gene Structure 6576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G055230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGTGGAGACAGCAAAGCCCTGCTATGAAGATCAAAGTCAAGGACCTGGTCAGCTCCGGTCAGCTCGAGTTCATAATGGGGGCATGTGTATGCACGACGAGGCCACCCCGCATTACATTGACTTGATTGATCAGACTACCCTAGGGCATCAATTTATACTGAAAGAGTTTGGTCTGACTCCTCGAGTTGGCTGGCAGATTGATCCTTTTGGCCATTCTGCTGTTCAAGCTTACTTGCTTGGTGCTGAGTTAGGTTTCGATTCCCTCTTTTTCGCTCGAATTGATTACCAGATAGAGCAGTCCGGCTCAGGGATAAGACTCTTGAGGTTATATGGCAGGATTTTCACTGGCGTATTCCCCAGGCACTATGATCCTCCAGATGGTTTCGTATTTGAAATAAATGATGTGTCACCTCCTATTCAGGATGACATTCTCCTGTTTGACTACAATGTTCAAGAGCGAGTCAATGACTTTGTTGCAGCTGCTTTAGCTCAGGCTAATGTGACAAGAACAAATCATATTATGTGGACCATGGGGACAGATTTTCGCTACCAATATGGAAATTCATGGTTCAGGCAGATGGACAAATTCATTCATTATGTCAACAAGGATGGGCGCGTCAATGCATTTTATTCAACTCCATCAATCTACACTGATGCAAAATATGCAGCAGATGAGCAATGGCCTCTGAAAACTGATGATTTCTTTCCATATGCGGATCATCCAAATGCATATTGGACCGGTTATTTTACAAGTCGTCCGGCCTTTAAAGGTTATGTCCGAAGTTTGAGTGGTTACTATCTGGCAGCAAGGCAGTTGGAATTCTTCAAAGGCAGGAGTGATTCTGGAGCAAATACTGATGCATTAGCTGATGCTTTGGCTATTGTTCAACATCATGATGCAGTTAGTGGTACACAACGACAGCATGTTGCTGCTGATTATGCAATGCGATTATCAATAGGTTATTTGGAGGCTGAAAAGTTGGTCGCCTCTTCTCTTGCTTACCTTTCTGAGTCAGAACCAAGTTCTGGACAGAAGCGCATAGCCACAAAATTTGAACAGTGCCCTCTTCTAAACATCAGTTATTGTCCTCCATCAGAAACTGCTTTATCCGATGGGAAAAGCTTGGTTGTTGTTATCTACAATCCTCTTGGTTGGAAGAGAGAGGAAGTAATATGGATCCCTGTTTCCAATGAACGGGTCACTGTTCAGGATTCTAGCCAAAGAAAAATTGATGCTCAGCTTTTACCTCTCTCAAATGCCACTTTGAAATTAAGAAGTTACTATGTCCGAGCATATTTGGGTATTACCCCAAGTGAACTGCCCAAATATTGGCTAGCTTTTTCAGTAACGGTACCACCACTTGGTTTCAGCTCTTACATTGTCTCAAGTGCAAATCAGACAGATAAAGATCGAAGCTCAACTATCTCCAAAGTATACACATCAGAAGGAAATACAAATAAGACCATACAAGTTGGGCAAGGGAGTTTAAAGCTACTTTATTCTGCAGAAGGAAAGCTTGCTCGTTATATTAACAGCAGAAATATGGTTACAGCGGTTGCTGAACAATCATATAGTTATTATACTGGAAACGATGGAACTGACAAAGATCCTCAGGCCTCTGGGGCATATGTATTTAGACCGAATGGCACAGTTGTTATAAAATCTGAAGAAAAGGTTTCTCTCACCGTGATGCGAGGCCCATTATTGGATGAAGTGCATCAGCAGATCAATCCATGGGTATCTCAGATTACAAGGTTATACAAAGGAAAGGAGCATGCTGAAGTAGAGTTCACTATTGGGCCTATACCCGTGGATGATGGTATTGGAAAAGAAATCACAACTCAACTTACAACAGCCATGAAGACCAATAAGACATTCTACACAGATTCCAATGGTCGCGACTTCATTAAGCGGATTCGAAATTTCAGGACAGATTGGGACCTACAAGTGAACCAACCAATTGCTGGAAATTATTACCCGATTAATCTTGGAATTTATGTGCAAGATAGCACTACAGAACTTTCAGTCTTGGTTGATCGTGCAGTGGGAGGATCTAGCTTAGTAGATGGTCAAGTAGAGCTGATGCTCCACAGGAGGCTAACTCATGATGATATCAGAGGTGTTGGCGAGATACTAAATGAAACGGTTTGCGTTTCTGAGAAGTGTGAAGGTTTAACTATCCAAGGGAAGTTTTATGTCAGAATCGATCCTGTTGGAGAAGGTTCTAAGTGGCGTCGCACAGCAGGCCAAGAGATAAGTGCTCCACTTTTATTGGCCTTCGCCGAACAGGAAGGAAATGATTGGATGAATTCTCATGTATCAACATTTTCTGGAATTGACCCTTCCTATGCCTTACCAGACAATGTTGCTGTGATAACCCTCCAGGAACTTGAAAATGGGAAAGTACTGCTTCGTTTGGCTCACCTATACGAGACTGGCGAGGACAAGGACTATTCTGTATTGGCAAATGTGGAACTAAGAAAGTTGTTTCCAAGGAAGAAGATCAGCAAAGTGACAGAGATGAGTTTATCTGCCAATCAAGAAAGAGCTGAAATGGAGAAGAGGAGATTAGTTTGGAAAGTGGAAGGTTCTGCCGCAGCCGAAGAGTCTAAGGTGGTGAGGGGAGGCGCTGTTGACCCTGCAAAACTGGTGGTGGAACTAGCACCAATGGAAATACGCACTTTCCTTATTGACCTGGATTACCTCCATATGTATGGTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005774 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009505 GO:0009987 GO:0015923 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030312 GO:0031090 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

908

Amino Acids

101.91

Weight (kDa)

5.67

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	24 - 239	1.1e-43	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	244 - 336	1.4e-19	Alpha mannosidase middle domain
Laman-like_dom	PF21260	411 - 462	7.3e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	494 - 707	2.9e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	789 - 896	6.1e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009766)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26720 AT3G26720 AT3G26720 AT3G26720 AT3G26720
fragaria_vesca	FvH4_6g54280 FvH4_6g54280 FvH4_6g54280 FvH4_6g54280
malus_domestica	MD09G1002800.v1.1
prunus_persica	Prupe.3G313400_v2.0.a1 Prupe.3G313400_v2.0.a1
pyrus_communis	pycom111g00230
rosa_chinensis	RchiOBHm_Chr2g0176491
rosa_laevigata	RLG00000022417
rosa_roxburghii	Rroxscaffold_2G00076340
rosa_rugosa	Rorug02G0595100
rosa_samantha	Rh2AG673400 Rh2BG684700 Rh2DG698300
rosa_wichuraiana	Rw2G055230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1661
Acc65I	GGTACC	1 cut(s)	1354
AccB1I	GGYRCC	1 cut(s)	1354
AccB7I	CCANNNNNTGG	4 cut(s)	1028, 1332, 1364, 1967
AccI	GTMKAC	1 cut(s)	1437
AccII	CGCG	2 cut(s)	618, 1902
AciI	CCGC	5 cut(s)	110, 716, 1556, 1915, 2598
AclWI	GGATC	8 cut(s)	197, 365, 726, 1174, 1187, 1604, 2062, 2204
AcoI	YGGCCR	1 cut(s)	211
AcsI	RAATTY	7 cut(s)	562, 587, 821, 1052, 1924, 1995, 2311
AcuI	CTGAAG	2 cut(s)	1689, 1803
AcyI	GRCGYC	1 cut(s)	2236
AfaI	GTAC	3 cut(s)	913, 1356, 2409
AfiI	CCNNNNNNNGG	9 cut(s)	188, 309, 380, 1028, 1197, 1332, 1364, 1708, 1967
AgeI	ACCGGT	1 cut(s)	740
AgsI	TTSAA	9 cut(s)	227, 395, 455, 636, 829, 890, 1058, 1270, 2396
AjnI	CCWGG	4 cut(s)	46, 359, 2385, 2697
AjuI	GAANNNNNNNTTGG	2 cut(s)	1862, 1894
Alw21I	GWGCWC	1 cut(s)	2266
Alw26I	GTCTC	3 cut(s)	2, 1390, 2429
AlwI	GGATC	8 cut(s)	197, 365, 726, 1174, 1187, 1604, 2062, 2204
AlwNI	CAGNNNCTG	1 cut(s)	1103
Ama87I	CYCGRG	2 cut(s)	66, 183
AoxI	GGCC	9 cut(s)	102, 211, 683, 762, 1617, 1699, 1802, 2248, 2277
ApeKI	GCWGC	6 cut(s)	479, 482, 668, 806, 932, 2600
ApoI	RAATTY	7 cut(s)	562, 587, 821, 1052, 1924, 1995, 2311
AseI	ATTAAT	1 cut(s)	1986
AsiGI	ACCGGT	1 cut(s)	740
Asp718I	GGTACC	1 cut(s)	1354
AspA2I	CCTAGG	1 cut(s)	142
AspLEI	GCGC	3 cut(s)	618, 1041, 2633
AspS9I	GGNCC	6 cut(s)	44, 528, 738, 1700, 1802, 1942
AsuHPI	GGTGA	4 cut(s)	402, 1678, 2417, 2632
AsuII	TTCGAA	1 cut(s)	1921
AsuNHI	GCTAGC	1 cut(s)	1336
AvaI	CYCGRG	2 cut(s)	66, 183
AvaII	GGWCC	4 cut(s)	44, 528, 738, 1942
AvrII	CCTAGG	1 cut(s)	142
AxyI	CCTNAGG	1 cut(s)	1614
BaeGI	GKGCMC	1 cut(s)	1067
BalI	TGGCCA	1 cut(s)	213
BamHI	GGATCC	1 cut(s)	1179
BanI	GGYRCC	1 cut(s)	1354
BbsI	GAAGAC	1 cut(s)	1871
Bbv12I	GWGCWC	1 cut(s)	2266
BbvI	GCAGC	6 cut(s)	469, 491, 680, 818, 919, 2612
BccI	CCATC	9 cut(s)	374, 574, 605, 649, 1102, 1109, 1589, 1814, 2063
BciT130I	CCWGG	4 cut(s)	48, 361, 2387, 2699
BclI	TGATCA	1 cut(s)	130
BcoDI	GTCTC	3 cut(s)	2, 1390, 2429
BfaI	CTAG	5 cut(s)	143, 1218, 1337, 2058, 2663
BfmI	CTRYAG	2 cut(s)	1506, 2016
BfoI	RGCGCY	1 cut(s)	2634
BglI	GCCNNNNNGGC	1 cut(s)	1625
BisI	GCNGC	7 cut(s)	480, 483, 669, 807, 933, 2598, 2601
BlnI	CCTAGG	1 cut(s)	142
BlpI	GCTNAGC	1 cut(s)	1239
BlsI	GCNGC	7 cut(s)	481, 484, 670, 808, 934, 2599, 2602
BmcAI	AGTACT	1 cut(s)	2409
Bme1390I	CCNGG	4 cut(s)	48, 361, 2387, 2699
Bme18I	GGWCC	4 cut(s)	44, 528, 738, 1942
BmeT110I	CYCGRG	2 cut(s)	66, 183
BmgT120I	GGNCC	6 cut(s)	44, 528, 738, 1700, 1802, 1942
BmiI	GGNNCC	4 cut(s)	1181, 1356, 1943, 2722
BmrFI	CCNGG	4 cut(s)	48, 361, 2387, 2699
BmsI	GCATC	9 cut(s)	157, 646, 850, 862, 889, 1225, 1683, 1729, 2076
BmtI	GCTAGC	1 cut(s)	1340
BpiI	GAAGAC	1 cut(s)	1871
BplI	GAGNNNNNCTC	2 cut(s)	2089, 2121
BpmI	CTGGAG	3 cut(s)	360, 867, 2369
Bpu10I	CCTNAGC	2 cut(s)	307, 492
Bpu1102I	GCTNAGC	1 cut(s)	1239
Bpu14I	TTCGAA	1 cut(s)	1921
BpuEI	CTTGAG	2 cut(s)	343, 1372
Bsa29I	ATCGAT	1 cut(s)	2208
BsaBI	GATNNNNATC	1 cut(s)	1410
BsaHI	GRCGYC	1 cut(s)	2236
BsaJI	CCNNGG	7 cut(s)	142, 359, 531, 1736, 1812, 2185, 2495
BsaWI	WCCGGW	2 cut(s)	57, 740
BsaXI	ACNNNNNCTCC	2 cut(s)	2619, 2649
Bsc4I	CCNNNNNNNGG	9 cut(s)	188, 309, 380, 1028, 1197, 1332, 1364, 1708, 1967
Bse118I	RCCGGY	1 cut(s)	740
Bse1I	ACTGG	4 cut(s)	352, 1591, 2443, 2655
Bse21I	CCTNAGG	1 cut(s)	1614
Bse3DI	GCAATG	2 cut(s)	686, 951
Bse8I	GATNNNNATC	1 cut(s)	1410
BseBI	CCWGG	4 cut(s)	48, 361, 2387, 2699
BseCI	ATCGAT	1 cut(s)	2208
BseDI	CCNNGG	7 cut(s)	142, 359, 531, 1736, 1812, 2185, 2495
BseGI	GGATG	6 cut(s)	430, 616, 721, 1717, 1822, 2313
BseJI	GATNNNNATC	1 cut(s)	1410
BseLI	CCNNNNNNNGG	9 cut(s)	188, 309, 380, 1028, 1197, 1332, 1364, 1708, 1967
BseMI	GCAATG	2 cut(s)	686, 951
BseMII	CTCAG	8 cut(s)	237, 321, 506, 1002, 1253, 1628, 1760, 2151
BseNI	ACTGG	4 cut(s)	352, 1591, 2443, 2655
BseRI	GAGGAG	2 cut(s)	171, 2581
BseSI	GKGCMC	1 cut(s)	1067
BseXI	GCAGC	6 cut(s)	469, 491, 680, 818, 919, 2612
BsgI	GTGCAG	1 cut(s)	2064
Bsh1236I	CGCG	2 cut(s)	618, 1902
BshFI	GGCC	9 cut(s)	104, 213, 685, 764, 1619, 1701, 1804, 2250, 2279
BshNI	GGYRCC	1 cut(s)	1354
BshTI	ACCGGT	1 cut(s)	740
BshVI	ATCGAT	1 cut(s)	2208
BsiHKAI	GWGCWC	1 cut(s)	2266
BsiHKCI	CYCGRG	2 cut(s)	66, 183
BsiSI	CCGG	4 cut(s)	58, 303, 741, 761
BslFI	GGGAC	2 cut(s)	550, 1955
BslI	CCNNNNNNNGG	9 cut(s)	188, 309, 380, 1028, 1197, 1332, 1364, 1708, 1967
BsmAI	GTCTC	3 cut(s)	2, 1390, 2429
BsmFI	GGGAC	2 cut(s)	550, 1955
BsnI	GGCC	9 cut(s)	104, 213, 685, 764, 1619, 1701, 1804, 2250, 2279
BsoBI	CYCGRG	2 cut(s)	66, 183
Bsp119I	TTCGAA	1 cut(s)	1921
Bsp1286I	GDGCHC	2 cut(s)	1067, 2266
Bsp1720I	GCTNAGC	1 cut(s)	1239
Bsp19I	CCATGG	2 cut(s)	531, 1736
Bsp68I	TCGCGA	1 cut(s)	1902
BspACI	CCGC	5 cut(s)	110, 716, 1556, 1915, 2598
BspANI	GGCC	9 cut(s)	104, 213, 685, 764, 1619, 1701, 1804, 2250, 2279
BspCNI	CTCAG	8 cut(s)	238, 320, 505, 1003, 1252, 1627, 1759, 2152
BspDI	ATCGAT	1 cut(s)	2208
BspFNI	CGCG	2 cut(s)	618, 1902
BspHI	TCATGA	2 cut(s)	895, 2107
BspLI	GGNNCC	4 cut(s)	1181, 1356, 1943, 2722
BspMAI	CTGCAG	1 cut(s)	1510
BspOI	GCTAGC	1 cut(s)	1340
BspPI	GGATC	8 cut(s)	197, 365, 726, 1174, 1187, 1604, 2062, 2204
BspT104I	TTCGAA	1 cut(s)	1921
BspT107I	GGYRCC	1 cut(s)	1354
BsrDI	GCAATG	2 cut(s)	686, 951
BsrFI	RCCGGY	1 cut(s)	740
BsrI	ACTGG	4 cut(s)	352, 1591, 2443, 2655
BssAI	RCCGGY	1 cut(s)	740
BssECI	CCNNGG	7 cut(s)	142, 359, 531, 1736, 1812, 2185, 2495
BssNAI	GTATAC	1 cut(s)	1438
BssNI	GRCGYC	1 cut(s)	2236
BssT1I	CCWWGG	5 cut(s)	142, 531, 1736, 2185, 2495
Bst1107I	GTATAC	1 cut(s)	1438
Bst2UI	CCWGG	4 cut(s)	48, 361, 2387, 2699
Bst4CI	ACNGT	6 cut(s)	1062, 1207, 1354, 1654, 1690, 2149
Bst6I	CTCTTC	5 cut(s)	997, 1074, 1154, 2558, 2601
BstACI	GRCGYC	1 cut(s)	2236
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstBI	TTCGAA	1 cut(s)	1921
BstC8I	GCNNGC	5 cut(s)	194, 1338, 1521, 1779, 2248
BstDSI	CCRYGG	3 cut(s)	531, 1736, 1812
BstF5I	GGATG	6 cut(s)	430, 616, 721, 1717, 1822, 2313
BstFNI	CGCG	2 cut(s)	618, 1902
BstH2I	RGCGCY	1 cut(s)	2634
BstHHI	GCGC	3 cut(s)	618, 1041, 2633
BstMAI	GTCTC	3 cut(s)	2, 1390, 2429
BstMWI	GCNNNNNNNGC	6 cut(s)	227, 488, 665, 941, 1625, 2247
BstNI	CCWGG	4 cut(s)	48, 361, 2387, 2699
BstNSI	RCATGY	3 cut(s)	88, 929, 1781
BstSCI	CCNGG	4 cut(s)	46, 359, 2385, 2697
BstSFI	CTRYAG	2 cut(s)	1506, 2016
BstSLI	GKGCMC	1 cut(s)	1067
BstUI	CGCG	2 cut(s)	618, 1902
BstV1I	GCAGC	6 cut(s)	469, 491, 680, 818, 919, 2612
BstV2I	GAAGAC	1 cut(s)	1871
BstX2I	RGATCY	3 cut(s)	1179, 1609, 2054
BstXI	CCANNNNNNTGG	1 cut(s)	2717
BstYI	RGATCY	3 cut(s)	1179, 1609, 2054
BstZ17I	GTATAC	1 cut(s)	1438
Bsu15I	ATCGAT	1 cut(s)	2208
Bsu36I	CCTNAGG	1 cut(s)	1614
BsuRI	GGCC	9 cut(s)	104, 213, 685, 764, 1619, 1701, 1804, 2250, 2279
BsuTUI	ATCGAT	1 cut(s)	2208
BtgI	CCRYGG	3 cut(s)	531, 1736, 1812
BtsCI	GGATG	6 cut(s)	430, 616, 721, 1717, 1822, 2313
BtsI	GCAGTG	1 cut(s)	2052
BtsIMutI	CAGTG	5 cut(s)	345, 651, 1067, 1203, 2052
BtuMI	TCGCGA	1 cut(s)	1902
Cac8I	GCNNGC	5 cut(s)	194, 1338, 1521, 1779, 2248
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	2 cut(s)	895, 2107
CfoI	GCGC	3 cut(s)	618, 1041, 2633
Cfr10I	RCCGGY	1 cut(s)	740
Cfr13I	GGNCC	6 cut(s)	44, 528, 738, 1700, 1802, 1942
ClaI	ATCGAT	1 cut(s)	2208
CseI	GACGC	2 cut(s)	607, 2225
CsiI	ACCWGGT	1 cut(s)	46
Csp6I	GTAC	3 cut(s)	912, 1355, 2408
CspAI	ACCGGT	1 cut(s)	740
CspCI	CAANNNNNGTGG	6 cut(s)	1036, 1071, 2256, 2291, 2634, 2669
CviQI	GTAC	3 cut(s)	912, 1355, 2408
DraI	TTTAAA	2 cut(s)	769, 1491
EaeI	YGGCCR	1 cut(s)	211
Eam1104I	CTCTTC	5 cut(s)	997, 1074, 1154, 2558, 2601
EarI	CTCTTC	5 cut(s)	997, 1074, 1154, 2558, 2601
Eco130I	CCWWGG	5 cut(s)	142, 531, 1736, 2185, 2495
Eco147I	AGGCCT	1 cut(s)	1619
Eco32I	GATATC	1 cut(s)	2116
Eco47I	GGWCC	4 cut(s)	44, 528, 738, 1942
Eco57I	CTGAAG	2 cut(s)	1689, 1803
Eco81I	CCTNAGG	1 cut(s)	1614
Eco88I	CYCGRG	2 cut(s)	66, 183
EcoO109I	RGGNCCY	2 cut(s)	44, 1942
EcoRI	GAATTC	2 cut(s)	821, 2311
EcoRII	CCWGG	4 cut(s)	46, 359, 2385, 2697
EcoRV	GATATC	1 cut(s)	2116
EcoT14I	CCWWGG	5 cut(s)	142, 531, 1736, 2185, 2495
EcoT22I	ATGCAT	3 cut(s)	628, 733, 865
ErhI	CCWWGG	5 cut(s)	142, 531, 1736, 2185, 2495
FalI	AAGNNNNNCTT	4 cut(s)	1503, 1535, 2397, 2429
FaqI	GGGAC	2 cut(s)	550, 1955
FauI	CCCGC	1 cut(s)	117
FauNDI	CATATG	3 cut(s)	712, 1630, 2712
FbaI	TGATCA	1 cut(s)	130
FblI	GTMKAC	1 cut(s)	1437
Fnu4HI	GCNGC	7 cut(s)	480, 483, 669, 807, 933, 2598, 2601
FokI	GGATG	6 cut(s)	437, 623, 708, 1724, 1829, 2320
Fsp4HI	GCNGC	7 cut(s)	480, 483, 669, 807, 933, 2598, 2601
FspBI	CTAG	5 cut(s)	143, 1218, 1337, 2058, 2663
GlaI	GCGC	3 cut(s)	617, 1040, 2632
GluI	GCNGC	7 cut(s)	480, 483, 669, 807, 933, 2598, 2601
GsuI	CTGGAG	3 cut(s)	360, 867, 2369
HaeII	RGCGCY	1 cut(s)	2634
HaeIII	GGCC	9 cut(s)	104, 213, 685, 764, 1619, 1701, 1804, 2250, 2279
HapII	CCGG	4 cut(s)	58, 303, 741, 761
HgaI	GACGC	2 cut(s)	607, 2225
HhaI	GCGC	3 cut(s)	618, 1041, 2633
Hin1I	GRCGYC	1 cut(s)	2236
Hin6I	GCGC	3 cut(s)	616, 1039, 2631
HinP1I	GCGC	3 cut(s)	616, 1039, 2631
HincII	GTYRAC	2 cut(s)	604, 2638
HindII	GTYRAC	2 cut(s)	604, 2638
HindIII	AAGCTT	3 cut(s)	228, 1123, 1517
HpaII	CCGG	4 cut(s)	58, 303, 741, 761
HphI	GGTGA	4 cut(s)	402, 1678, 2417, 2632
Hpy166II	GTNNAC	8 cut(s)	528, 604, 914, 1319, 1438, 1794, 1954, 2638
Hpy8I	GTNNAC	8 cut(s)	528, 604, 914, 1319, 1438, 1794, 1954, 2638
Hpy99I	CGWCG	2 cut(s)	101, 2241
HpyAV	CCTTC	8 cut(s)	1442, 1505, 2165, 2216, 2288, 2290, 2355, 2582
HpyCH4III	ACNGT	6 cut(s)	1062, 1207, 1354, 1654, 1690, 2149
HpyF10VI	GCNNNNNNNGC	6 cut(s)	227, 488, 665, 941, 1625, 2247
Hsp92I	GRCGYC	1 cut(s)	2236
HspAI	GCGC	3 cut(s)	616, 1039, 2631
KpnI	GGTACC	1 cut(s)	1358
Ksp22I	TGATCA	1 cut(s)	130
LmnI	GCTCC	5 cut(s)	60, 848, 1774, 2094, 2269
Lsp1109I	GCAGC	6 cut(s)	469, 491, 680, 818, 919, 2612
LweI	GCATC	9 cut(s)	157, 646, 850, 862, 889, 1225, 1683, 1729, 2076
MabI	ACCWGGT	1 cut(s)	46
MaeI	CTAG	5 cut(s)	143, 1218, 1337, 2058, 2663
MaeIII	GTNAC	8 cut(s)	408, 502, 794, 1201, 1280, 1348, 1549, 2515
MfeI	CAATTG	1 cut(s)	1962
MflI	RGATCY	3 cut(s)	1179, 1609, 2054
MhlI	GDGCHC	2 cut(s)	1067, 2266
MlsI	TGGCCA	1 cut(s)	213
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	5 cut(s)	172, 312, 471, 1022, 2618
MmeI	TCCRAC	3 cut(s)	798, 1136, 2197
Mox20I	TGGCCA	1 cut(s)	213
Mph1103I	ATGCAT	3 cut(s)	628, 733, 865
MscI	TGGCCA	1 cut(s)	213
MseI	TTAA	7 cut(s)	768, 1274, 1490, 1533, 1911, 1986, 2177
MslI	CAYNNNNRTG	5 cut(s)	89, 726, 897, 1691, 2715
Msp20I	TGGCCA	1 cut(s)	213
MspA1I	CMGCKG	2 cut(s)	482, 1556
MspI	CCGG	4 cut(s)	58, 303, 741, 761
MspR9I	CCNGG	4 cut(s)	48, 361, 2387, 2699
MunI	CAATTG	1 cut(s)	1962
MvaI	CCWGG	4 cut(s)	48, 361, 2387, 2699
MvnI	CGCG	2 cut(s)	618, 1902
MwoI	GCNNNNNNNGC	6 cut(s)	227, 488, 665, 941, 1625, 2247
NcoI	CCATGG	2 cut(s)	531, 1736
NdeI	CATATG	3 cut(s)	712, 1630, 2712
NheI	GCTAGC	1 cut(s)	1336
NlaIV	GGNNCC	4 cut(s)	1181, 1356, 1943, 2722
NmuCI	GTSAC	4 cut(s)	408, 502, 1201, 2515
NruI	TCGCGA	1 cut(s)	1902
NsiI	ATGCAT	3 cut(s)	628, 733, 865
NspI	RCATGY	3 cut(s)	88, 929, 1781
NspV	TTCGAA	1 cut(s)	1921
PaeI	GCATGC	1 cut(s)	1781
PaeR7I	CTCGAG	2 cut(s)	66, 183
PagI	TCATGA	2 cut(s)	895, 2107
PceI	AGGCCT	1 cut(s)	1619
PcsI	WCGNNNNNNNCGW	1 cut(s)	2439
PfeI	GAWTC	6 cut(s)	260, 842, 1214, 1889, 1918, 2205
PflFI	GACNNNGTC	1 cut(s)	48
PflMI	CCANNNNNTGG	4 cut(s)	1028, 1332, 1364, 1967
PfoI	TCCNGGA	1 cut(s)	2385
PinAI	ACCGGT	1 cut(s)	740
PkrI	GCNGC	7 cut(s)	481, 484, 670, 808, 934, 2599, 2602
PleI	GAGTC	5 cut(s)	172, 312, 470, 1021, 2617
PpsI	GAGTC	5 cut(s)	172, 312, 470, 1021, 2617
PpuMI	RGGWCCY	2 cut(s)	44, 1942
PshBI	ATTAAT	1 cut(s)	1986
PsiI	TTATAA	1 cut(s)	1661
Psp5II	RGGWCCY	2 cut(s)	44, 1942
Psp6I	CCWGG	4 cut(s)	46, 359, 2385, 2697
PspGI	CCWGG	4 cut(s)	46, 359, 2385, 2697
PspN4I	GGNNCC	4 cut(s)	1181, 1356, 1943, 2722
PspPI	GGNCC	6 cut(s)	44, 528, 738, 1700, 1802, 1942
PspPPI	RGGWCCY	2 cut(s)	44, 1942
PspXI	VCTCGAGB	2 cut(s)	66, 183
PstI	CTGCAG	1 cut(s)	1510
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	3 cut(s)	1179, 1609, 2054
PsyI	GACNNNGTC	1 cut(s)	48
PvuII	CAGCTG	1 cut(s)	482
RruI	TCGCGA	1 cut(s)	1902
RsaI	GTAC	3 cut(s)	913, 1356, 2409
RsaNI	GTAC	3 cut(s)	912, 1355, 2408
RseI	CAYNNNNRTG	5 cut(s)	89, 726, 897, 1691, 2715
SaqAI	TTAA	7 cut(s)	768, 1274, 1490, 1533, 1911, 1986, 2177
SatI	GCNGC	7 cut(s)	480, 483, 669, 807, 933, 2598, 2601
Sau96I	GGNCC	6 cut(s)	44, 528, 738, 1700, 1802, 1942
ScaI	AGTACT	1 cut(s)	2409
SchI	GAGTC	5 cut(s)	172, 312, 471, 1022, 2618
ScrFI	CCNGG	4 cut(s)	48, 361, 2387, 2699
SduI	GDGCHC	2 cut(s)	1067, 2266
SexAI	ACCWGGT	1 cut(s)	46
SfaNI	GCATC	9 cut(s)	157, 646, 850, 862, 889, 1225, 1683, 1729, 2076
SfcI	CTRYAG	2 cut(s)	1506, 2016
Sfr274I	CTCGAG	2 cut(s)	66, 183
SfuI	TTCGAA	1 cut(s)	1921
SinI	GGWCC	4 cut(s)	44, 528, 738, 1942
SlaI	CTCGAG	2 cut(s)	66, 183
SmiMI	CAYNNNNRTG	5 cut(s)	89, 726, 897, 1691, 2715
SmlI	CTYRAG	4 cut(s)	66, 183, 322, 1387
SmoI	CTYRAG	4 cut(s)	66, 183, 322, 1387
SphI	GCATGC	1 cut(s)	1781
SseBI	AGGCCT	1 cut(s)	1619
SsiI	CCGC	5 cut(s)	110, 716, 1556, 1915, 2598
SspI	AATATT	1 cut(s)	1331
SspMI	CTAG	5 cut(s)	143, 1218, 1337, 2058, 2663
StuI	AGGCCT	1 cut(s)	1619
StyD4I	CCNGG	4 cut(s)	46, 359, 2385, 2697
StyI	CCWWGG	5 cut(s)	142, 531, 1736, 2185, 2495
TaaI	ACNGT	6 cut(s)	1062, 1207, 1354, 1654, 1690, 2149
TaqI	TCGA	7 cut(s)	67, 184, 258, 277, 1414, 1921, 2208
TaqII	GACCGA	1 cut(s)	1657
TatI	WGTACW	1 cut(s)	2407
TauI	GCSGC	1 cut(s)	2600
TfiI	GAWTC	6 cut(s)	260, 842, 1214, 1889, 1918, 2205
Tru1I	TTAA	7 cut(s)	768, 1274, 1490, 1533, 1911, 1986, 2177
Tru9I	TTAA	7 cut(s)	768, 1274, 1490, 1533, 1911, 1986, 2177
TscAI	CASTG	5 cut(s)	352, 658, 1067, 1210, 2052
TseFI	GTSAC	4 cut(s)	408, 502, 1201, 2515
TseI	GCWGC	6 cut(s)	479, 482, 668, 806, 932, 2600
Tsp45I	GTSAC	4 cut(s)	408, 502, 1201, 2515
TspRI	CASTG	5 cut(s)	352, 658, 1067, 1210, 2052
Tth111I	GACNNNGTC	1 cut(s)	48
Van91I	CCANNNNNTGG	4 cut(s)	1028, 1332, 1364, 1967
VpaK11BI	GGWCC	4 cut(s)	44, 528, 738, 1942
VspI	ATTAAT	1 cut(s)	1986
XapI	RAATTY	7 cut(s)	562, 587, 821, 1052, 1924, 1995, 2311
XceI	RCATGY	3 cut(s)	88, 929, 1781
XcmI	CCANNNNNNNNNTGG	2 cut(s)	732, 1964
XhoI	CTCGAG	2 cut(s)	66, 183
XmaJI	CCTAGG	1 cut(s)	142
XmiI	GTMKAC	1 cut(s)	1437
XspI	CTAG	5 cut(s)	143, 1218, 1337, 2058, 2663
ZrmI	AGTACT	1 cut(s)	2409
Zsp2I	ATGCAT	3 cut(s)	628, 733, 865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.