MD09G1017100.v1.1

F-box protein SKIP14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005923
Physical Location & Seq
Reverse (-)
1064370 .. 1067615
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1017100.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGGCTTTGAATCTCTCTCGTGGCTCAATCTTGCCTGCGTATCCGTCCTCGGAGCATAGTTCGTGTTCTTCTGTTAAGTACCCTAATGGGTATGTGATGGATCAATTCAAGGGAAAGGATTTGGATGGTATTTGTGATGATCCGTCAAGTTCTTGGAGTTCCAATTTGGATTTGAAGCATACCCATTGTAAGAGCGAATTGGGTGGTTATTGTGACCGTTCTGCCGATGATATTGTGGATCGATTGCCTGTCGATCCTTTTGGAATGGAGATTAGGTCTACGTTTACGGCGATCACTGGTTGGTTTCAAGGTTTCGAACCAAATTATGAATCTTGTAGCTCTGGGTGGGATGAAGGAAAGGCAAAGATGGTTGATGGCGGATTGTTTGCCGGTCTGAATTGGGTTTGGAATAGCGCTGTGTGGTATCAGCCGGAAGTTTGTGACCTGAAATTTGATGGAGTATCAATCCCATATGACAGCTTTGATGGGTTCGGGATCAATGATGGAGGTTTTGCATTGGATGACAGTGTGGAGGAGTTTCTGAGTTTTAGCTATGTGGGGGACTTAGATGTTTTTGATGGAGCTAAGCAACTGCAGGTTGGAACTAAGGAAATACAGGGTTGTGGCATCATGTGTCCCGAGAGTGAGGCGAGTGCTCCGCATGATGCTATGTTTTATGCATTGCGTTTTCTTGGTGTTAAGGACCTTCTGTCGGTTGAAAGGGTTTGCAAGTCTTTTCGTAATGCAGTTAGAAGTGACCCTCTTCCATGGAGGAGTATTGTCATCGATTGGCCGTTGAATGAACGCATCACCGATGATGTTCTTGTAAAACTAACAAGCAGGGCTCAAGGCACACTTCAAACCTTGGGTTTAGTGCATTGCGTTAAGATCACTGATGGTGGGCTGCAGGGTGTGTTTGACAGCAACCCGAGGTTGACAAAGTTAAGTGTTCCCGGATGTCTTAAAATCAGTGTCGAGTGCATCTTGTTAAACATAAGGACCTTGAAGTCTGCAGGCAACCCCGGAATAAAGCAGTTAAGAATTGGTGGGCTCTCTGGTATAACAGACAAGCAGTTCGAAGAGTTGAAATTCTTGCTGGCCACAGATAACCATATCCAGCCTGGAGCCCCGAAACCTCGGTTTTTTAATGGTGGGTTGTCGTATGGCTCTTGTGATGATGATTGTCCCATCGACATCGAAGCATGCCCCAGGTGCCAGAGACTTAGTTTAGTTTATGATTGCCCTGCAAAGAGTTGCCAAGGGAAACAACCTGCTGATCAGAAGTGCAGGGCTTGCACCCTCTGCATAGCTCGCTGTATTAGTTGCGGGTGCTGCCTTAAAGACTGCGATTATGAGGAGACATTTTGTCTAGACTTGCTTTGCTTGCATTGTTTGGAGAGCCTACTCAACTGTCAAGAGAAGCATGGAGAAAAGGGCGCTCCCAAGTGCGCCATATTCTGTCAGGAAAACAGGTACCAATTTTGCCTTGTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

499

Amino Acids

54.76

Weight (kDa)

4.94

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 220 - 262 8e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1006
Acc36I ACCTGC 2 cut(s) 586, 1279
Acc65I GGTACC 1 cut(s) 1473
AccB1I GGYRCC 2 cut(s) 1212, 1473
AccI GTMKAC 1 cut(s) 278
AciI CCGC 3 cut(s) 378, 659, 1326
AclWI GGATC 5 cut(s) 108, 134, 246, 248, 503
AcoI YGGCCR 2 cut(s) 791, 1098
AcsI RAATTY 2 cut(s) 449, 1088
AfaI GTAC 2 cut(s) 80, 1475
AfeI AGCGCT 1 cut(s) 415
AfiI CCNNNNNNNGG 1 cut(s) 712
AgsI TTSAA 9 cut(s) 10, 109, 175, 308, 719, 799, 860, 1006, 1087
AhdI GACNNNNNGTC 2 cut(s) 709, 1365
AjnI CCWGG 2 cut(s) 1120, 1208
AluBI AGCT 5 cut(s) 339, 480, 552, 584, 1310
AluI AGCT 5 cut(s) 339, 480, 552, 584, 1310
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 2 cut(s) 1213, 1352
AlwI GGATC 5 cut(s) 108, 134, 246, 248, 503
Ama87I CYCGRG 2 cut(s) 638, 928
Aor51HI AGCGCT 1 cut(s) 415
AoxI GGCC 2 cut(s) 791, 1098
ApeKI GCWGC 2 cut(s) 904, 1332
ApoI RAATTY 2 cut(s) 449, 1088
ArsI GACNNNNNNTTYG 4 cut(s) 434, 466, 1058, 1090
Asp718I GGTACC 1 cut(s) 1473
AspLEI GCGC 3 cut(s) 416, 1439, 1451
AspS9I GGNCC 2 cut(s) 703, 999
AsuC2I CCSGG 2 cut(s) 954, 1023
AsuHPI GGTGA 1 cut(s) 802
AsuII TTCGAA 2 cut(s) 315, 1077
AvaI CYCGRG 2 cut(s) 638, 928
AvaII GGWCC 2 cut(s) 703, 999
BalI TGGCCA 1 cut(s) 1100
BanI GGYRCC 2 cut(s) 1212, 1473
BanII GRGCYC 3 cut(s) 847, 1053, 1129
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 2 cut(s) 891, 1319
BceAI ACGGC 2 cut(s) 303, 778
BciT130I CCWGG 2 cut(s) 1122, 1210
BciVI GTATCC 1 cut(s) 51
BclI TGATCA 1 cut(s) 1276
BcnI CCSGG 2 cut(s) 954, 1023
BcoDI GTCTC 2 cut(s) 1213, 1352
BfaI CTAG 1 cut(s) 1370
BfmI CTRYAG 3 cut(s) 593, 905, 1011
BfoI RGCGCY 2 cut(s) 417, 1440
BfuAI ACCTGC 2 cut(s) 586, 1279
BfuI GTATCC 1 cut(s) 51
BglI GCCNNNNNGGC 1 cut(s) 1491
BisI GCNGC 2 cut(s) 905, 1333
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 2 cut(s) 906, 1334
Bme1390I CCNGG 4 cut(s) 954, 1023, 1122, 1210
Bme18I GGWCC 2 cut(s) 703, 999
BmeRI GACNNNNNGTC 2 cut(s) 709, 1365
BmeT110I CYCGRG 2 cut(s) 638, 928
BmgT120I GGNCC 2 cut(s) 703, 999
BmiI GGNNCC 3 cut(s) 1126, 1214, 1475
BmrFI CCNGG 4 cut(s) 954, 1023, 1122, 1210
BmsI GCATC 4 cut(s) 636, 655, 816, 990
BpmI CTGGAG 1 cut(s) 1143
Bpu1102I GCTNAGC 1 cut(s) 585
Bpu14I TTCGAA 2 cut(s) 315, 1077
BpuEI CTTGAG 1 cut(s) 831
BpuMI CCSGG 2 cut(s) 954, 1023
Bsa29I ATCGAT 2 cut(s) 241, 786
BsaJI CCNNGG 8 cut(s) 48, 767, 864, 929, 1021, 1136, 1208, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 712
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 2 cut(s) 680, 877
BseBI CCWGG 2 cut(s) 1122, 1210
BseCI ATCGAT 2 cut(s) 241, 786
BseDI CCNNGG 8 cut(s) 48, 767, 864, 929, 1021, 1136, 1208, 1258
BseGI GGATG 4 cut(s) 130, 355, 526, 962
BseLI CCNNNNNNNGG 1 cut(s) 712
BseMI GCAATG 2 cut(s) 680, 877
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 3 cut(s) 548, 787, 1370
BseXI GCAGC 2 cut(s) 891, 1319
BsgI GTGCAG 1 cut(s) 1306
BshFI GGCC 2 cut(s) 793, 1100
BshNI GGYRCC 2 cut(s) 1212, 1473
BshVI ATCGAT 2 cut(s) 241, 786
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 2 cut(s) 638, 928
BsiSI CCGG 4 cut(s) 390, 431, 954, 1023
BslFI GGGAC 3 cut(s) 575, 621, 1170
BslI CCNNNNNNNGG 1 cut(s) 712
BsmAI GTCTC 2 cut(s) 1213, 1352
BsmFI GGGAC 3 cut(s) 575, 621, 1170
BsnI GGCC 2 cut(s) 793, 1100
BsoBI CYCGRG 2 cut(s) 638, 928
Bsp119I TTCGAA 2 cut(s) 315, 1077
Bsp1286I GDGCHC 4 cut(s) 658, 847, 1053, 1129
Bsp143I GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
Bsp1720I GCTNAGC 1 cut(s) 585
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 3 cut(s) 378, 659, 1326
BspANI GGCC 2 cut(s) 793, 1100
BspCNI CTCAG 1 cut(s) 534
BspDI ATCGAT 2 cut(s) 241, 786
BspLI GGNNCC 3 cut(s) 1126, 1214, 1475
BspMAI CTGCAG 3 cut(s) 597, 909, 1015
BspMI ACCTGC 2 cut(s) 586, 1279
BspPI GGATC 5 cut(s) 108, 134, 246, 248, 503
BspT104I TTCGAA 2 cut(s) 315, 1077
BspT107I GGYRCC 2 cut(s) 1212, 1473
BsrDI GCAATG 2 cut(s) 680, 877
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 8 cut(s) 48, 767, 864, 929, 1021, 1136, 1208, 1258
BssMI GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 767, 864, 1258
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 1122, 1210
Bst4CI ACNGT 3 cut(s) 218, 527, 1412
Bst6I CTCTTC 2 cut(s) 768, 1074
BstAPI GCANNNNNTGC 2 cut(s) 1293, 1302
BstBI TTCGAA 2 cut(s) 315, 1077
BstC8I GCNNGC 7 cut(s) 36, 1015, 1098, 1204, 1294, 1312, 1385
BstDEI CTNAG 5 cut(s) 542, 565, 585, 606, 1223
BstDSI CCRYGG 1 cut(s) 767
BstF5I GGATG 4 cut(s) 130, 355, 526, 962
BstH2I RGCGCY 2 cut(s) 417, 1440
BstHHI GCGC 3 cut(s) 416, 1439, 1451
BstKTI GATC 8 cut(s) 103, 142, 241, 256, 294, 498, 891, 1279
BstMAI GTCTC 2 cut(s) 1213, 1352
BstMBI GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
BstMWI GCNNNNNNNGC 7 cut(s) 1212, 1293, 1302, 1311, 1332, 1384, 1491
BstNI CCWGG 2 cut(s) 1122, 1210
BstNSI RCATGY 1 cut(s) 1206
BstSCI CCNGG 4 cut(s) 952, 1021, 1120, 1208
BstSFI CTRYAG 3 cut(s) 593, 905, 1011
BstV1I GCAGC 2 cut(s) 891, 1319
Bsu15I ATCGAT 2 cut(s) 241, 786
BsuI GTATCC 1 cut(s) 51
BsuRI GGCC 2 cut(s) 793, 1100
BsuTUI ATCGAT 2 cut(s) 241, 786
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 4 cut(s) 130, 355, 526, 962
BtsIMutI CAGTG 4 cut(s) 294, 532, 891, 976
BveI ACCTGC 2 cut(s) 586, 1279
Cac8I GCNNGC 7 cut(s) 36, 1015, 1098, 1204, 1294, 1312, 1385
CfoI GCGC 3 cut(s) 416, 1439, 1451
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 703, 999
ClaI ATCGAT 2 cut(s) 241, 786
Csp6I GTAC 2 cut(s) 79, 1474
CviAII CATG 5 cut(s) 631, 662, 768, 1203, 1424
CviQI GTAC 2 cut(s) 79, 1474
DdeI CTNAG 5 cut(s) 542, 565, 585, 606, 1223
DpnI GATC 8 cut(s) 102, 141, 240, 255, 293, 497, 890, 1278
DpnII GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
DrdI GACNNNNNNGTC 1 cut(s) 1006
DriI GACNNNNNGTC 2 cut(s) 709, 1365
DseDI GACNNNNNNGTC 1 cut(s) 1006
EaeI YGGCCR 2 cut(s) 791, 1098
Eam1104I CTCTTC 2 cut(s) 768, 1074
Eam1105I GACNNNNNGTC 2 cut(s) 709, 1365
EarI CTCTTC 2 cut(s) 768, 1074
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 3 cut(s) 767, 864, 1258
Eco24I GRGCYC 3 cut(s) 847, 1053, 1129
Eco47I GGWCC 2 cut(s) 703, 999
Eco47III AGCGCT 1 cut(s) 415
Eco88I CYCGRG 2 cut(s) 638, 928
EcoO109I RGGNCCY 2 cut(s) 703, 999
EcoRII CCWGG 2 cut(s) 1120, 1208
EcoT14I CCWWGG 3 cut(s) 767, 864, 1258
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 3 cut(s) 847, 1053, 1129
ErhI CCWWGG 3 cut(s) 767, 864, 1258
FaeI CATG 5 cut(s) 634, 665, 771, 1206, 1427
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 3 cut(s) 575, 621, 1170
FatI CATG 5 cut(s) 630, 661, 767, 1202, 1423
FauI CCCGC 1 cut(s) 1319
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 1276
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 2 cut(s) 905, 1333
FokI GGATG 4 cut(s) 137, 362, 533, 969
FriOI GRGCYC 3 cut(s) 847, 1053, 1129
Fsp4HI GCNGC 2 cut(s) 905, 1333
FspBI CTAG 1 cut(s) 1370
GlaI GCGC 3 cut(s) 415, 1438, 1450
GluI GCNGC 2 cut(s) 905, 1333
GsuI CTGGAG 1 cut(s) 1143
HaeII RGCGCY 2 cut(s) 417, 1440
HaeIII GGCC 2 cut(s) 793, 1100
HapII CCGG 4 cut(s) 390, 431, 954, 1023
HhaI GCGC 3 cut(s) 416, 1439, 1451
Hin1II CATG 5 cut(s) 634, 665, 771, 1206, 1427
Hin6I GCGC 3 cut(s) 414, 1437, 1449
HinP1I GCGC 3 cut(s) 414, 1437, 1449
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HinfI GANTC 2 cut(s) 10, 329
HpaII CCGG 4 cut(s) 390, 431, 954, 1023
HphI GGTGA 1 cut(s) 802
Hpy166II GTNNAC 3 cut(s) 279, 285, 936
Hpy188I TCNGA 4 cut(s) 52, 396, 543, 1281
Hpy188III TCNNGA 5 cut(s) 493, 638, 1370, 1415, 1463
Hpy8I GTNNAC 3 cut(s) 279, 285, 936
HpyAV CCTTC 2 cut(s) 347, 716
HpyCH4III ACNGT 3 cut(s) 218, 527, 1412
HpyCH4IV ACGT 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 7 cut(s) 1212, 1293, 1302, 1311, 1332, 1384, 1491
HpyF3I CTNAG 5 cut(s) 542, 565, 585, 606, 1223
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 5 cut(s) 634, 665, 771, 1206, 1427
HspAI GCGC 3 cut(s) 414, 1437, 1449
KpnI GGTACC 1 cut(s) 1477
Ksp22I TGATCA 1 cut(s) 1276
Kzo9I GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
LmnI GCTCC 5 cut(s) 52, 581, 661, 1124, 1444
Lsp1109I GCAGC 2 cut(s) 891, 1319
LweI GCATC 4 cut(s) 636, 655, 816, 990
MaeI CTAG 1 cut(s) 1370
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 3 cut(s) 212, 440, 755
MalI GATC 8 cut(s) 102, 141, 240, 255, 293, 497, 890, 1278
MboI GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
MboII GAAGA 3 cut(s) 60, 755, 1091
MhlI GDGCHC 4 cut(s) 658, 847, 1053, 1129
MlsI TGGCCA 1 cut(s) 1100
MluCI AATT 9 cut(s) 104, 163, 197, 322, 397, 449, 1041, 1088, 1478
MluNI TGGCCA 1 cut(s) 1100
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 1100
Mph1103I ATGCAT 1 cut(s) 682
MscI TGGCCA 1 cut(s) 1100
MseI TTAA 9 cut(s) 75, 699, 885, 944, 963, 989, 1037, 1146, 1338
Msp20I TGGCCA 1 cut(s) 1100
MspI CCGG 4 cut(s) 390, 431, 954, 1023
MspR9I CCNGG 4 cut(s) 954, 1023, 1122, 1210
MvaI CCWGG 2 cut(s) 1122, 1210
MwoI GCNNNNNNNGC 7 cut(s) 1212, 1293, 1302, 1311, 1332, 1384, 1491
NciI CCSGG 2 cut(s) 954, 1023
NcoI CCATGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 472
NdeII GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
NlaIII CATG 5 cut(s) 634, 665, 771, 1206, 1427
NlaIV GGNNCC 3 cut(s) 1126, 1214, 1475
NmuCI GTSAC 3 cut(s) 212, 440, 755
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 1206
NspV TTCGAA 2 cut(s) 315, 1077
PaeI GCATGC 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PfeI GAWTC 2 cut(s) 10, 329
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 2 cut(s) 906, 1334
PpuMI RGGWCCY 2 cut(s) 703, 999
Psp5II RGGWCCY 2 cut(s) 703, 999
Psp6I CCWGG 2 cut(s) 1120, 1208
PspGI CCWGG 2 cut(s) 1120, 1208
PspN4I GGNNCC 3 cut(s) 1126, 1214, 1475
PspPI GGNCC 2 cut(s) 703, 999
PspPPI RGGWCCY 2 cut(s) 703, 999
PstI CTGCAG 3 cut(s) 597, 909, 1015
RsaI GTAC 2 cut(s) 80, 1475
RsaNI GTAC 2 cut(s) 79, 1474
SaqAI TTAA 9 cut(s) 75, 699, 885, 944, 963, 989, 1037, 1146, 1338
SatI GCNGC 2 cut(s) 905, 1333
Sau3AI GATC 8 cut(s) 100, 139, 238, 253, 291, 495, 888, 1276
Sau96I GGNCC 2 cut(s) 703, 999
ScrFI CCNGG 4 cut(s) 954, 1023, 1122, 1210
SduI GDGCHC 4 cut(s) 658, 847, 1053, 1129
SfaNI GCATC 4 cut(s) 636, 655, 816, 990
SfcI CTRYAG 3 cut(s) 593, 905, 1011
SfuI TTCGAA 2 cut(s) 315, 1077
SinI GGWCC 2 cut(s) 703, 999
SmlI CTYRAG 1 cut(s) 846
SmoI CTYRAG 1 cut(s) 846
SphI GCATGC 1 cut(s) 1206
Sse9I AATT 9 cut(s) 104, 163, 197, 322, 397, 449, 1041, 1088, 1478
SsiI CCGC 3 cut(s) 378, 659, 1326
SspMI CTAG 1 cut(s) 1370
StyD4I CCNGG 4 cut(s) 952, 1021, 1120, 1208
StyI CCWWGG 3 cut(s) 767, 864, 1258
TaaI ACNGT 3 cut(s) 218, 527, 1412
TaiI ACGT 1 cut(s) 284
TaqI TCGA 8 cut(s) 241, 252, 315, 786, 975, 1077, 1191, 1197
TasI AATT 9 cut(s) 104, 163, 197, 322, 397, 449, 1041, 1088, 1478
TfiI GAWTC 2 cut(s) 10, 329
Tru1I TTAA 9 cut(s) 75, 699, 885, 944, 963, 989, 1037, 1146, 1338
Tru9I TTAA 9 cut(s) 75, 699, 885, 944, 963, 989, 1037, 1146, 1338
TscAI CASTG 4 cut(s) 301, 532, 898, 976
TseFI GTSAC 3 cut(s) 212, 440, 755
TseI GCWGC 2 cut(s) 904, 1332
Tsp45I GTSAC 3 cut(s) 212, 440, 755
TspDTI ATGAA 3 cut(s) 342, 366, 816
TspGWI ACGGA 2 cut(s) 33, 132
TspRI CASTG 4 cut(s) 301, 532, 898, 976
VpaK11BI GGWCC 2 cut(s) 703, 999
XapI RAATTY 2 cut(s) 449, 1088
XbaI TCTAGA 1 cut(s) 1369
XceI RCATGY 1 cut(s) 1206
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XmiI GTMKAC 1 cut(s) 278
XspI CTAG 1 cut(s) 1370
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.