Rh2AG655900

F-box protein SKIP14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005923
Physical Location & Seq
Reverse (-)
87269692 .. 87273601
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG655900.1

Sequence Viewer

Length: 1482 bp
ATGGCTTTGAATCTGTCTCGTGGCTCAGTTCTGCCTAAGTATCCTTCCCCAGAGCATAGTTTATGTTCTTCTGTTAGGTGTTCTAATGGGTATGTGAGGGATCAATGTAAAGAGAAGAGTTTGGATGGTGATGCATCGAGTTCGAATTCGGAGTTGAAGAAGAATACCCATTACAAGGGTGAATTGGGTGGCTATTGTGAGCCTTCTGTTGATGATATTCTTGATAGGTTGCCTGTTGATCCTTTTGGGATGGATATGAGGTCTACTTTTACGGCGATTAGTGGTTGGCTTGAGGATTTTGAGAAGGGGTGTGAGTCTTATGGGTTTGGGGTGGATGAGCAGAAGAAGAAAGTGGTGGATGGTGGGTTGTTTGCTGGTCTGAATTGGGTTTGGAATAGCGCTGTGATATTTCAGCCGGAACTGGGTGAGGTGAAGTTTGATGGAATATCGATTCCATATGATAGCTTTGATGGGTTTGGGATCAATGATGGAGGTTTTGTATTGGACGACAATGTGGAGGAGTTTCTGGGTTTTGGCCATGTGGTGAACTTGGATGTTGGTTATGGAGCTAAGGAACTGCCGGTTGGAACTATGGAAAGGCCAGAATGTAGCATAAAGAATTTTGATAGTGAAGGGGGTGCTCCACATGATGCTATGTTTTATGCATTGGGTTATCTCGGCGTTCAGGATCTTCTTTCAGTTCAACGGGTTTGCAGGTCTTTGAATCATGCAGTCAAAAGTGATCCTCTTTTATGGAGGAGCCTTAACATTGATTGGCCGTTGAATGAGAGGATCACTGATGATGCTCTTGTAAATTTAACTAGCAGGGCTCAGGGTAATCTTCAAACCTTAAGTCTAGTGCATTGTGCATGGATTACCGATATTGGGCTGCATCGCGTGTTTGACAGCAATCCAAGGCTGACAAAATTAAGTATTCCAGGATGTATAAAAATAAGTGTTGAGAGCATCTTGTTAAACCTAAGGACCTTGAAGTCTGCAGGCAAACCTGGAATAAAGCAGTTAAGAATTGGAGGTCTCTCTGGTATAACAGACAAGCAGTTCGAAGAGTTAAAATTCTTGCTGGGTGCAGATAACCATATACAGGTTAAAGCCCCAAAACCACGGTATTTTCATGGTGGGCTATCGTACCTCTCTTGTGATGATGATTGCGCCATCGACATTGAAGCATGCCCCAGGTGCCAGAGACTAAGTTTAGTTTATGATTGCCCAGCAGAGAGTTGCCAAGGGAAATATCAGAAGGCTCAGATGTGCAGGGCTTGCATACTCTGCATAACTCGCTGTATTAGCTGTGGGTGTTGCCTCGGAGACTGCGATTATGTGGAGACGTTTTGCCTACAGTTGCTTTGTTTGGAATGTTTGGAGAACCTACTCAACTGTCAAGAGAGGCATGGAGAAAATGGTGCTACCAAATGTGCCATATTTTGTCAGGAGAGCAGGTATCAGTTTTGCCTTTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

493

Amino Acids

54.5

Weight (kDa)

5.11

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 215 - 257 9.1e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 991
Acc36I ACCTGC 2 cut(s) 705, 1446
AccB1I GGYRCC 1 cut(s) 1197
AccI GTMKAC 1 cut(s) 263
AccII CGCG 1 cut(s) 897
AclWI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
AcoI YGGCCR 2 cut(s) 535, 776
AcsI RAATTY 4 cut(s) 145, 619, 814, 1073
AfaI GTAC 1 cut(s) 1148
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 4 cut(s) 175, 422, 885, 1102
AflII CTTAAG 1 cut(s) 850
AgsI TTSAA 8 cut(s) 10, 157, 704, 724, 784, 845, 991, 1184
AjnI CCWGG 3 cut(s) 937, 1006, 1193
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AluBI AGCT 3 cut(s) 465, 569, 1308
AluI AGCT 3 cut(s) 465, 569, 1308
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 5 cut(s) 21, 1040, 1198, 1320, 1337
AlwI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 3 cut(s) 535, 599, 776
ApeKI GCWGC 1 cut(s) 889
ApoI RAATTY 4 cut(s) 145, 619, 814, 1073
ArsI GACNNNNNNTTYG 4 cut(s) 838, 870, 1043, 1075
AspLEI GCGC 2 cut(s) 401, 1172
AspS9I GGNCC 1 cut(s) 984
AsuHPI GGTGA 5 cut(s) 140, 191, 437, 442, 556
AsuII TTCGAA 2 cut(s) 143, 1062
AvaII GGWCC 1 cut(s) 984
AxyI CCTNAGG 1 cut(s) 980
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 832
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 876
BccI CCATC 7 cut(s) 119, 244, 353, 434, 464, 482, 1181
BceAI ACGGC 2 cut(s) 288, 763
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 3 cut(s) 939, 1008, 1195
BciVI GTATCC 1 cut(s) 51
BcoDI GTCTC 5 cut(s) 21, 1040, 1198, 1320, 1337
BfaI CTAG 2 cut(s) 822, 857
BfmI CTRYAG 2 cut(s) 996, 1355
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 850
BfuAI ACCTGC 2 cut(s) 705, 1446
BfuI GTATCC 1 cut(s) 51
BglI GCCNNNNNGGC 1 cut(s) 1476
BisI GCNGC 1 cut(s) 890
BlsI GCNGC 1 cut(s) 891
Bme1390I CCNGG 3 cut(s) 939, 1008, 1195
Bme18I GGWCC 1 cut(s) 984
BmgT120I GGNCC 1 cut(s) 984
BmiI GGNNCC 2 cut(s) 761, 1199
BmrFI CCNGG 3 cut(s) 939, 1008, 1195
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
BmuI ACTGGG 1 cut(s) 431
Bpu10I CCTNAGC 2 cut(s) 570, 831
Bpu14I TTCGAA 2 cut(s) 143, 1062
BpuEI CTTGAG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 449
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BsaXI ACNNNNNCTCC 4 cut(s) 483, 513, 1442, 1472
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 422, 885, 1102
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 1 cut(s) 426
Bse21I CCTNAGG 1 cut(s) 980
BseBI CCWGG 3 cut(s) 939, 1008, 1195
BseCI ATCGAT 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BseGI GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BseLI CCNNNNNNNGG 4 cut(s) 175, 422, 885, 1102
BseMII CTCAG 3 cut(s) 39, 845, 1277
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 2 cut(s) 533, 772
BseXI GCAGC 1 cut(s) 876
BseYI CCCAGC 2 cut(s) 1081, 1228
BsgI GTGCAG 2 cut(s) 1107, 1291
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 3 cut(s) 537, 601, 778
BshNI GGYRCC 1 cut(s) 1197
BshVI ATCGAT 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 2 cut(s) 416, 581
BslI CCNNNNNNNGG 4 cut(s) 175, 422, 885, 1102
BsmAI GTCTC 5 cut(s) 21, 1040, 1198, 1320, 1337
BsmBI CGTCTC 1 cut(s) 1337
BsnI GGCC 3 cut(s) 537, 601, 778
Bso31I GGTCTC 1 cut(s) 1040
Bsp119I TTCGAA 2 cut(s) 143, 1062
Bsp1286I GDGCHC 2 cut(s) 643, 832
Bsp143I GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BspANI GGCC 3 cut(s) 537, 601, 778
BspCNI CTCAG 3 cut(s) 38, 844, 1276
BspDI ATCGAT 1 cut(s) 449
BspFNI CGCG 1 cut(s) 897
BspLI GGNNCC 2 cut(s) 761, 1199
BspMAI CTGCAG 1 cut(s) 1000
BspMI ACCTGC 2 cut(s) 705, 1446
BspPI GGATC 6 cut(s) 108, 233, 488, 696, 737, 800
BspT104I TTCGAA 2 cut(s) 143, 1062
BspT107I GGYRCC 1 cut(s) 1197
BspTI CTTAAG 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 1040
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 5 cut(s) 914, 1121, 1193, 1243, 1321
BssMI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 914, 1243
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 939, 1008, 1195
Bst4CI ACNGT 3 cut(s) 1125, 1359, 1397
Bst6I CTCTTC 2 cut(s) 110, 1059
BstAFI CTTAAG 1 cut(s) 850
BstAPI GCANNNNNTGC 2 cut(s) 1278, 1287
BstBI TTCGAA 2 cut(s) 143, 1062
BstC8I GCNNGC 3 cut(s) 1000, 1189, 1279
BstDEI CTNAG 7 cut(s) 25, 36, 570, 831, 980, 1208, 1263
BstDSI CCRYGG 1 cut(s) 1121
BstF5I GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BstFNI CGCG 1 cut(s) 897
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 401, 1172
BstKTI GATC 6 cut(s) 103, 241, 483, 691, 745, 795
BstMAI GTCTC 5 cut(s) 21, 1040, 1198, 1320, 1337
BstMBI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
BstMWI GCNNNNNNNGC 6 cut(s) 1197, 1278, 1287, 1296, 1305, 1476
BstNI CCWGG 3 cut(s) 939, 1008, 1195
BstNSI RCATGY 1 cut(s) 1191
BstSCI CCNGG 3 cut(s) 937, 1006, 1193
BstSFI CTRYAG 2 cut(s) 996, 1355
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 1 cut(s) 876
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 449
Bsu36I CCTNAGG 1 cut(s) 980
BsuI GTATCC 1 cut(s) 51
BsuRI GGCC 3 cut(s) 537, 601, 778
BsuTUI ATCGAT 1 cut(s) 449
BtgI CCRYGG 1 cut(s) 1121
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 6 cut(s) 130, 255, 340, 364, 559, 947
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 2 cut(s) 705, 1446
Cac8I GCNNGC 3 cut(s) 1000, 1189, 1279
CfoI GCGC 2 cut(s) 401, 1172
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 984
ClaI ATCGAT 1 cut(s) 449
Csp6I GTAC 1 cut(s) 1147
CviAII CATG 7 cut(s) 539, 647, 728, 870, 1133, 1188, 1409
CviQI GTAC 1 cut(s) 1147
DdeI CTNAG 7 cut(s) 25, 36, 570, 831, 980, 1208, 1263
DpnI GATC 6 cut(s) 102, 240, 482, 690, 744, 794
DpnII GATC 6 cut(s) 100, 238, 480, 688, 742, 792
DrdI GACNNNNNNGTC 1 cut(s) 991
DseDI GACNNNNNNGTC 1 cut(s) 991
EaeI YGGCCR 2 cut(s) 535, 776
Eam1104I CTCTTC 2 cut(s) 110, 1059
EarI CTCTTC 2 cut(s) 110, 1059
Eco130I CCWWGG 2 cut(s) 914, 1243
Eco24I GRGCYC 1 cut(s) 832
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 1 cut(s) 984
Eco47III AGCGCT 1 cut(s) 400
Eco81I CCTNAGG 1 cut(s) 980
EcoO109I RGGNCCY 1 cut(s) 984
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 937, 1006, 1193
EcoT14I CCWWGG 2 cut(s) 914, 1243
EcoT22I ATGCAT 2 cut(s) 136, 667
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 2 cut(s) 914, 1243
Esp3I CGTCTC 1 cut(s) 1337
FaeI CATG 7 cut(s) 542, 650, 731, 873, 1136, 1191, 1412
FatI CATG 7 cut(s) 538, 646, 727, 869, 1132, 1187, 1408
FauNDI CATATG 1 cut(s) 457
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 1 cut(s) 890
FokI GGATG 6 cut(s) 137, 262, 347, 371, 566, 954
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 1 cut(s) 890
FspBI CTAG 2 cut(s) 822, 857
GlaI GCGC 2 cut(s) 400, 1171
GluI GCNGC 1 cut(s) 890
GsaI CCCAGC 2 cut(s) 1085, 1232
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 3 cut(s) 537, 601, 778
HapII CCGG 2 cut(s) 416, 581
HhaI GCGC 2 cut(s) 401, 1172
Hin1II CATG 7 cut(s) 542, 650, 731, 873, 1136, 1191, 1412
Hin6I GCGC 2 cut(s) 399, 1170
HinP1I GCGC 2 cut(s) 399, 1170
HinfI GANTC 4 cut(s) 10, 314, 451, 724
HpaII CCGG 2 cut(s) 416, 581
HphI GGTGA 5 cut(s) 140, 191, 437, 442, 556
Hpy166II GTNNAC 2 cut(s) 264, 547
Hpy188I TCNGA 5 cut(s) 151, 381, 1257, 1266, 1325
Hpy188III TCNNGA 4 cut(s) 221, 686, 1400, 1448
Hpy8I GTNNAC 2 cut(s) 264, 547
HpyAV CCTTC 5 cut(s) 54, 213, 298, 626, 1252
HpyCH4III ACNGT 3 cut(s) 1125, 1359, 1397
HpyCH4IV ACGT 1 cut(s) 1346
HpyF10VI GCNNNNNNNGC 6 cut(s) 1197, 1278, 1287, 1296, 1305, 1476
HpyF3I CTNAG 7 cut(s) 25, 36, 570, 831, 980, 1208, 1263
HpySE526I ACGT 1 cut(s) 1346
Hsp92II CATG 7 cut(s) 542, 650, 731, 873, 1136, 1191, 1412
HspAI GCGC 2 cut(s) 399, 1170
Kzo9I GATC 6 cut(s) 100, 238, 480, 688, 742, 792
LmnI GCTCC 3 cut(s) 566, 646, 759
Lsp1109I GCAGC 1 cut(s) 876
LweI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
MaeI CTAG 2 cut(s) 822, 857
MaeII ACGT 1 cut(s) 1346
MalI GATC 6 cut(s) 102, 240, 482, 690, 744, 794
MboI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
MboII GAAGA 9 cut(s) 60, 127, 169, 172, 355, 358, 683, 833, 1076
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 2 cut(s) 643, 832
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 8 cut(s) 145, 182, 382, 619, 814, 926, 1026, 1073
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 565
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 136, 667
MscI TGGCCA 1 cut(s) 537
MseI TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
Msp20I TGGCCA 1 cut(s) 537
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 2 cut(s) 416, 581
MspR9I CCNGG 3 cut(s) 939, 1008, 1195
MvaI CCWGG 3 cut(s) 939, 1008, 1195
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 6 cut(s) 1197, 1278, 1287, 1296, 1305, 1476
NdeI CATATG 1 cut(s) 457
NdeII GATC 6 cut(s) 100, 238, 480, 688, 742, 792
NlaIII CATG 7 cut(s) 542, 650, 731, 873, 1136, 1191, 1412
NlaIV GGNNCC 2 cut(s) 761, 1199
NmeAIII GCCGAG 1 cut(s) 657
NsiI ATGCAT 2 cut(s) 136, 667
NspI RCATGY 1 cut(s) 1191
NspV TTCGAA 2 cut(s) 143, 1062
PaeI GCATGC 1 cut(s) 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 1329
PfeI GAWTC 3 cut(s) 10, 451, 724
PfoI TCCNGGA 1 cut(s) 937
PkrI GCNGC 1 cut(s) 891
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 984
Psp6I CCWGG 3 cut(s) 937, 1006, 1193
PspFI CCCAGC 2 cut(s) 1081, 1228
PspGI CCWGG 3 cut(s) 937, 1006, 1193
PspN4I GGNNCC 2 cut(s) 761, 1199
PspPI GGNCC 1 cut(s) 984
PspPPI RGGWCCY 1 cut(s) 984
PstI CTGCAG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 1 cut(s) 1148
RsaNI GTAC 1 cut(s) 1147
SaqAI TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
SatI GCNGC 1 cut(s) 890
Sau3AI GATC 6 cut(s) 100, 238, 480, 688, 742, 792
Sau96I GGNCC 1 cut(s) 984
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 939, 1008, 1195
SduI GDGCHC 2 cut(s) 643, 832
SfaNI GCATC 6 cut(s) 121, 143, 640, 793, 901, 975
SfcI CTRYAG 2 cut(s) 996, 1355
SfuI TTCGAA 2 cut(s) 143, 1062
SinI GGWCC 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 290, 850
SmoI CTYRAG 2 cut(s) 290, 850
SphI GCATGC 1 cut(s) 1191
Sse9I AATT 8 cut(s) 145, 182, 382, 619, 814, 926, 1026, 1073
SspMI CTAG 2 cut(s) 822, 857
StyD4I CCNGG 3 cut(s) 937, 1006, 1193
StyI CCWWGG 2 cut(s) 914, 1243
TaaI ACNGT 3 cut(s) 1125, 1359, 1397
TaiI ACGT 1 cut(s) 1349
TaqI TCGA 5 cut(s) 137, 143, 449, 1062, 1176
TasI AATT 8 cut(s) 145, 182, 382, 619, 814, 926, 1026, 1073
TfiI GAWTC 3 cut(s) 10, 451, 724
Tru1I TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
Tru9I TTAA 8 cut(s) 765, 818, 851, 929, 974, 1022, 1070, 1107
TscAI CASTG 1 cut(s) 802
TseI GCWGC 1 cut(s) 889
TspDTI ATGAA 1 cut(s) 1121
TspRI CASTG 1 cut(s) 802
Vha464I CTTAAG 1 cut(s) 850
VpaK11BI GGWCC 1 cut(s) 984
XapI RAATTY 4 cut(s) 145, 619, 814, 1073
XceI RCATGY 1 cut(s) 1191
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 2 cut(s) 822, 857
Zsp2I ATGCAT 2 cut(s) 136, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.