MD09G1019900.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015791
Physical Location & Seq
Forward (+)
1204419 .. 1206536
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1019900.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGCTCAGCTCTCACTTTTGCTTACAATTTTTCTCATAACTTCTTTAAGTGCAACTTTTGTTTCCTCTTCACAAGTTCAAGACCCTGAACGAGTAGTAGAGGAGGTCCATAGGAGCATCAATGCCTCGAGGAGGAACTTGGCCTACTTGTCTTGTGGAACTGGCAACCCAATTGATGATTGCTGGAGGTGTGATCCCAACTGGGAAAGAAACCGCCAGCGCTTTGCTGACTGTGCCATTGGCTTCGGCAAAGACGCCATCGGAGGAAGAAACGGTCGAATCTACGTCGTCACCGACTCCGGCGATGACGACCCAGTAAACCCTAAGCCAGGGACCCTAAGACACGCAGTGATCCAGGACGAGCCGTTGTGGATCATATTCAAAAGGGACATGGTGGTTCAGCTAAAACAAGAGCTAGTTATGAACTCGTTCAAGACCATCGACGGCAGAGGTGCGAGCGTCCACATTGCTGGAGGGCCATGCATCACCATACATTACGCTACCAACATCATCATCCATGGCATCCACATACACGACTGCAAACCGGCGGGTAATGGGAACATAAGAAACTCGCCGGAGCACTCGGGGTGGTGGACGGTGTCGGACGGCGACGGTATATCCATTTTTAATGGGCAGCATATATGGGTGGACCATTGCTCGCTGTCGAATTGCCATGATGGACTCATTGATGCCATACATGGATCCACGGCCATCACCATATCGAATAACTACATGACTCATCATGATAAGGTCATGCTTTTGGGACATAGTGATTCGTATACACAAGACAAGAACATGCAAGTCACTGTTGCCTTTAATCATTTTGGAGAAGGCCTTGTCCAAAGAATGCCAAGGTGTAGACATGGATATTTTCATGTGGTAAACAATGACTACACACACTGGGAAATGTACGCAATAGGAGGGAGTGCTTCCCCAACTATCCTCAGCCAAGGCAATAGGTTTCTTGCACCAGATAGACGTTTTGATAAAGAGGTGACAAAACATGAGGATGCACCAGAAAGTGAGTGGAGGAAGTGGAACTGGAGGTCAGAGGGGGATATGCTGTTGAACGGGGCGTTTTTCAGGCAATCAGGAGCAGGAGCCTCTTCAACCTATGCCAGGGCTTCCAGCCTCAGTGCAAGGCCTTCTTCTCTTGTGGGTTCCATCACCACCGCCGCCGGTGCCCTTAACTGCAGGAGGGGATCCCACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

405

Amino Acids

44.79

Weight (kDa)

6.71

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 137 - 325 4.9e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27400 AT3G27400
fragaria_vesca FvH4_6g52190
malus_domestica MD09G1019900.v1.1
prunus_persica Prupe.3G296600_v2.0.a1
pyrus_communis pycom111g01650
rosa_chinensis RchiOBHm_Chr2g0173681
rosa_laevigata RLG00000022214
rosa_multiflora Rmu_sc0002538.1_g000007
rosa_rugosa Rorug02G0572300
rosa_samantha Rh2AG651200 Rh2BG661600 Rh2CG626600 Rh2DG676900
rosa_wichuraiana Rw2G053440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 1181
AccI GTMKAC 2 cut(s) 779, 859
AciI CCGC 4 cut(s) 214, 548, 1173, 1176
AclWI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
AcoI YGGCCR 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 1 cut(s) 911
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 132, 329, 1119
AgsI TTSAA 5 cut(s) 80, 382, 433, 1069, 1110
AjnI CCWGG 3 cut(s) 328, 354, 1118
AluBI AGCT 3 cut(s) 10, 403, 415
AluI AGCT 3 cut(s) 10, 403, 415
Alw21I GWGCWC 1 cut(s) 582
AlwI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
Ama87I CYCGRG 2 cut(s) 127, 583
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 5 cut(s) 141, 476, 708, 832, 1142
ApeKI GCWGC 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 4 cut(s) 106, 333, 476, 649
AsuHPI GGTGA 5 cut(s) 283, 478, 706, 1006, 1159
AvaI CYCGRG 2 cut(s) 127, 583
AvaII GGWCC 3 cut(s) 106, 333, 649
BaeGI GKGCMC 1 cut(s) 1186
BamHI GGATCC 2 cut(s) 701, 1202
BanI GGYRCC 1 cut(s) 1181
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 1 cut(s) 646
BccI CCATC 5 cut(s) 266, 446, 671, 719, 1172
BceAI ACGGC 4 cut(s) 349, 460, 622, 723
BciT130I CCWGG 3 cut(s) 330, 356, 1120
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 1192
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 635, 1176
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 2 cut(s) 636, 1177
Bme1390I CCNGG 3 cut(s) 330, 356, 1120
Bme18I GGWCC 3 cut(s) 106, 333, 649
BmeT110I CYCGRG 2 cut(s) 127, 583
BmgT120I GGNCC 4 cut(s) 106, 333, 476, 649
BmiI GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
BmrFI CCNGG 3 cut(s) 330, 356, 1120
BmrI ACTGGG 3 cut(s) 211, 308, 910
BmsI GCATC 5 cut(s) 126, 492, 531, 679, 1000
BmuI ACTGGG 3 cut(s) 211, 308, 910
BpmI CTGGAG 3 cut(s) 205, 492, 1063
Bpu10I CCTNAGC 2 cut(s) 324, 944
Bpu1102I GCTNAGC 1 cut(s) 6
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 329, 1119
Bse118I RCCGGY 2 cut(s) 544, 1178
Bse1I ACTGG 5 cut(s) 166, 206, 314, 905, 1046
Bse3DI GCAATG 2 cut(s) 465, 652
BseBI CCWGG 3 cut(s) 330, 356, 1120
BseDI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
BseGI GGATG 3 cut(s) 513, 522, 1015
BseLI CCNNNNNNNGG 3 cut(s) 132, 329, 1119
BseMI GCAATG 2 cut(s) 465, 652
BseMII CTCAG 3 cut(s) 20, 958, 1147
BseNI ACTGG 5 cut(s) 166, 206, 314, 905, 1046
BseRI GAGGAG 2 cut(s) 116, 145
BseSI GKGCMC 1 cut(s) 1186
BseXI GCAGC 1 cut(s) 646
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BshNI GGYRCC 1 cut(s) 1181
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 2 cut(s) 127, 583
BsiSI CCGG 4 cut(s) 300, 545, 575, 1179
BslFI GGGAC 3 cut(s) 346, 401, 777
BslI CCNNNNNNNGG 3 cut(s) 132, 329, 1119
BsmFI GGGAC 3 cut(s) 346, 401, 777
BsmI GAATGC 1 cut(s) 852
BsnI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BsoBI CYCGRG 2 cut(s) 127, 583
Bsp1286I GDGCHC 2 cut(s) 582, 1186
Bsp143I GATC 5 cut(s) 193, 351, 372, 701, 1202
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 4 cut(s) 214, 548, 1173, 1176
BspANI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BspCNI CTCAG 3 cut(s) 19, 957, 1146
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
BspMAI CTGCAG 1 cut(s) 1196
BspPI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
BspT107I GGYRCC 1 cut(s) 1181
BsrDI GCAATG 2 cut(s) 465, 652
BsrFI RCCGGY 2 cut(s) 544, 1178
BsrI ACTGG 5 cut(s) 166, 206, 314, 905, 1046
BssAI RCCGGY 2 cut(s) 544, 1178
BssECI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
BssMI GATC 5 cut(s) 193, 351, 372, 701, 1202
BssNAI GTATAC 1 cut(s) 780
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 3 cut(s) 517, 851, 949
Bst1107I GTATAC 1 cut(s) 780
Bst2UI CCWGG 3 cut(s) 330, 356, 1120
Bst4CI ACNGT 5 cut(s) 233, 275, 598, 614, 808
Bst6I CTCTTC 2 cut(s) 73, 1111
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 3 cut(s) 218, 457, 659
BstDEI CTNAG 5 cut(s) 6, 324, 338, 944, 1133
BstDSI CCRYGG 2 cut(s) 517, 705
BstENI CCTNNNNNAGG 3 cut(s) 130, 327, 1117
BstF5I GGATG 3 cut(s) 513, 522, 1015
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 5 cut(s) 196, 354, 375, 704, 1205
BstMBI GATC 5 cut(s) 193, 351, 372, 701, 1202
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 233, 1181
BstNI CCWGG 3 cut(s) 330, 356, 1120
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 3 cut(s) 328, 354, 1118
BstSFI CTRYAG 1 cut(s) 1192
BstSLI GKGCMC 1 cut(s) 1186
BstV1I GCAGC 1 cut(s) 646
BstX2I RGATCY 2 cut(s) 701, 1202
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 2 cut(s) 701, 1202
BstZ17I GTATAC 1 cut(s) 780
BsuRI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BtgI CCRYGG 2 cut(s) 517, 705
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 3 cut(s) 513, 522, 1015
BtsI GCAGTG 2 cut(s) 354, 1207
BtsIMutI CAGTG 5 cut(s) 354, 804, 898, 1141, 1207
Cac8I GCNNGC 3 cut(s) 218, 457, 659
CciI TCATGA 1 cut(s) 742
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 2 cut(s) 544, 1178
Cfr13I GGNCC 4 cut(s) 106, 333, 476, 649
CseI GACGC 2 cut(s) 263, 448
Csp6I GTAC 1 cut(s) 910
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 5 cut(s) 6, 324, 338, 944, 1133
DpnI GATC 5 cut(s) 195, 353, 374, 703, 1204
DpnII GATC 5 cut(s) 193, 351, 372, 701, 1202
DraIII CACNNNGTG 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 2 cut(s) 73, 1111
EarI CTCTTC 2 cut(s) 73, 1111
Eco130I CCWWGG 3 cut(s) 517, 851, 949
Eco147I AGGCCT 2 cut(s) 834, 1144
Eco47I GGWCC 3 cut(s) 106, 333, 649
Eco47III AGCGCT 1 cut(s) 221
Eco88I CYCGRG 2 cut(s) 127, 583
EcoNI CCTNNNNNAGG 3 cut(s) 130, 327, 1117
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 328, 354, 1118
EcoT14I CCWWGG 3 cut(s) 517, 851, 949
EcoT22I ATGCAT 1 cut(s) 485
ErhI CCWWGG 3 cut(s) 517, 851, 949
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 1132, 1164
FaqI GGGAC 3 cut(s) 346, 401, 777
FauI CCCGC 1 cut(s) 541
FblI GTMKAC 2 cut(s) 779, 859
Fnu4HI GCNGC 2 cut(s) 635, 1176
FokI GGATG 3 cut(s) 500, 509, 1022
Fsp4HI GCNGC 2 cut(s) 635, 1176
FspBI CTAG 1 cut(s) 416
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 635, 1176
GsuI CTGGAG 3 cut(s) 205, 492, 1063
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 5 cut(s) 143, 478, 710, 834, 1144
HapII CCGG 4 cut(s) 300, 545, 575, 1179
HgaI GACGC 2 cut(s) 263, 448
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 255
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 5 cut(s) 279, 296, 681, 736, 773
HpaII CCGG 4 cut(s) 300, 545, 575, 1179
HphI GGTGA 5 cut(s) 283, 478, 706, 1006, 1159
Hpy166II GTNNAC 7 cut(s) 319, 463, 594, 649, 780, 860, 883
Hpy188I TCNGA 3 cut(s) 263, 604, 1051
Hpy188III TCNNGA 4 cut(s) 80, 433, 743, 1092
Hpy8I GTNNAC 7 cut(s) 319, 463, 594, 649, 780, 860, 883
Hpy99I CGWCG 3 cut(s) 290, 446, 614
HpyAV CCTTC 2 cut(s) 824, 1155
HpyCH4III ACNGT 5 cut(s) 233, 275, 598, 614, 808
HpyCH4IV ACGT 2 cut(s) 285, 979
HpyCH4V TGCA 8 cut(s) 53, 483, 540, 799, 968, 1013, 1139, 1194
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 1181
HpyF3I CTNAG 5 cut(s) 6, 324, 338, 944, 1133
HpySE526I ACGT 2 cut(s) 285, 979
Hsp92I GRCGYC 1 cut(s) 255
HspAI GCGC 1 cut(s) 220
KflI GGGWCCC 1 cut(s) 333
Kzo9I GATC 5 cut(s) 193, 351, 372, 701, 1202
LmnI GCTCC 4 cut(s) 114, 577, 1094, 1100
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 5 cut(s) 126, 492, 531, 679, 1000
MaeI CTAG 1 cut(s) 416
MaeII ACGT 2 cut(s) 285, 979
MaeIII GTNAC 3 cut(s) 289, 802, 994
MalI GATC 5 cut(s) 195, 353, 374, 703, 1204
MboI GATC 5 cut(s) 193, 351, 372, 701, 1202
MboII GAAGA 4 cut(s) 60, 279, 1098, 1140
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 2 cut(s) 701, 1202
MhlI GDGCHC 2 cut(s) 582, 1186
MluCI AATT 3 cut(s) 27, 171, 667
MlyI GAGTC 3 cut(s) 290, 675, 730
MmeI TCCRAC 1 cut(s) 582
Mph1103I ATGCAT 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 4 cut(s) 47, 627, 816, 1188
MslI CAYNNNNRTG 1 cut(s) 1008
MspI CCGG 4 cut(s) 300, 545, 575, 1179
MspR9I CCNGG 3 cut(s) 330, 356, 1120
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 852
MvaI CCWGG 3 cut(s) 330, 356, 1120
MwoI GCNNNNNNNGC 2 cut(s) 233, 1181
NcoI CCATGG 1 cut(s) 517
NdeII GATC 5 cut(s) 193, 351, 372, 701, 1202
NlaIV GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
NmuCI GTSAC 3 cut(s) 289, 802, 994
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 1 cut(s) 799
PaeR7I CTCGAG 1 cut(s) 127
PagI TCATGA 1 cut(s) 742
PceI AGGCCT 2 cut(s) 834, 1144
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 852
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 279, 773
PflFI GACNNNGTC 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 2 cut(s) 636, 1177
PleI GAGTC 3 cut(s) 290, 675, 730
PpsI GAGTC 3 cut(s) 290, 675, 730
PpuMI RGGWCCY 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 333
Psp6I CCWGG 3 cut(s) 328, 354, 1118
PspGI CCWGG 3 cut(s) 328, 354, 1118
PspN4I GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
PspPI GGNCC 4 cut(s) 106, 333, 476, 649
PspPPI RGGWCCY 1 cut(s) 333
PspXI VCTCGAGB 1 cut(s) 127
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PstI CTGCAG 1 cut(s) 1196
PsuI RGATCY 2 cut(s) 701, 1202
PsyI GACNNNGTC 1 cut(s) 598
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 1 cut(s) 1008
SaqAI TTAA 4 cut(s) 47, 627, 816, 1188
SatI GCNGC 2 cut(s) 635, 1176
Sau3AI GATC 5 cut(s) 193, 351, 372, 701, 1202
Sau96I GGNCC 4 cut(s) 106, 333, 476, 649
SchI GAGTC 3 cut(s) 290, 675, 730
ScrFI CCNGG 3 cut(s) 330, 356, 1120
SduI GDGCHC 2 cut(s) 582, 1186
SfaNI GCATC 5 cut(s) 126, 492, 531, 679, 1000
SfcI CTRYAG 1 cut(s) 1192
Sfr274I CTCGAG 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 1178
SinI GGWCC 3 cut(s) 106, 333, 649
SlaI CTCGAG 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 1008
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 3 cut(s) 27, 171, 667
SseBI AGGCCT 2 cut(s) 834, 1144
SsiI CCGC 4 cut(s) 214, 548, 1173, 1176
SspMI CTAG 1 cut(s) 416
StuI AGGCCT 2 cut(s) 834, 1144
StyD4I CCNGG 3 cut(s) 328, 354, 1118
StyI CCWWGG 3 cut(s) 517, 851, 949
TaaI ACNGT 5 cut(s) 233, 275, 598, 614, 808
TaiI ACGT 2 cut(s) 288, 982
TaqI TCGA 5 cut(s) 128, 277, 441, 665, 722
TasI AATT 3 cut(s) 27, 171, 667
TauI GCSGC 1 cut(s) 1178
TfiI GAWTC 2 cut(s) 279, 773
Tru1I TTAA 4 cut(s) 47, 627, 816, 1188
Tru9I TTAA 4 cut(s) 47, 627, 816, 1188
TscAI CASTG 5 cut(s) 354, 811, 905, 1141, 1214
TseFI GTSAC 3 cut(s) 289, 802, 994
TseI GCWGC 1 cut(s) 634
Tsp45I GTSAC 3 cut(s) 289, 802, 994
TspDTI ATGAA 2 cut(s) 437, 863
TspRI CASTG 5 cut(s) 354, 811, 905, 1141, 1214
Tth111I GACNNNGTC 1 cut(s) 598
VpaK11BI GGWCC 3 cut(s) 106, 333, 649
XagI CCTNNNNNAGG 3 cut(s) 130, 327, 1117
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 1023
XhoI CTCGAG 1 cut(s) 127
XmiI GTMKAC 2 cut(s) 779, 859
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.