Prupe.3G296600_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015791
Physical Location & Seq
Reverse (-)
26345295 .. 26347881
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G296600.1

Sequence Viewer

Length: 1218 bp
ATGGCTGGGCTCTCACTTTTGCTTATTTTCCTCATCATCACTTGTTTCCTAACTGCAACTTTTGCTTCCTCCTCACAACTTCATGACCCTGAACTGGTAGCACAGGAAGTCCATAGGAGCATCAATGCTTCTAGGAGGAACTTGGGCTACTTGTCATGTGGAACTGGCAACCCCATAGACGATTGCTGGAGGTGTGATCCCAACTGGGAACAAAACCGCCAGCGCTTAGCCGACTGCGCCATTGGGTTCGGCAAGGACGCCATCGGAGGAAGAAACGGTCGAATCTACGTAGTCACCGACTCTGGGGACGACGACCCAGTGAACCCTAGACCCGGAACCCTAAGACACGCAGTGATCCAAGACGAGCCATTGTGGATTATATTCAAAAGGGACATGGTGGTTCAGCTGAAGCAAGAGCTTGTAATGAACTCGTTCAAGACCATCGACGGTAGAGGAGCGAGCGTCCACATAGCCGGAGGGCCATGCATCACCATCCATTATGCCACCAACATCATCATCCATGGCATCAACATCCACGACTGTAAGCAGGCTGGGAACGGGAACATACGAGACTCGCCGCAGCATTCAGGGTGGTGGACTATATCGGACGGCGATGGGGTGTCCATATTTGGTGGGAAACATATATGGGTGGACCATTGCTCTCTCTCTAATTGCCATGATGGCCTCATTGATGCCATCCATGGATCCACAGCCATCACCATATCCAATAACTACATGACCCATCATGATAAGGTCATGCTTTTGGGGCACAGCGATTCCTATACGCAAGACAAGGGCATGCAGGTCACAGTTGCCTTTAATCATTTTGGAGAAGGCCTTGTCCAAAGAATGCCAAGGTGTAGACATGGATATTTTCATGTGGTGAACAATGACTACACACACTGGGAAATGTATGCAATTGGAGGGAGTGCAGCTCCAACTATCAACAGCCAAGGCAATAGATTTCTGGCGCCTAATACACGTTTCAATAAAGAGGTGACTAAACATGAGGATGCACCGGAAAGTGAGTGGAGGGGTTGGAACTGGAGGTCAGAGGGGGATCTGATGTTGAACGGGGCATATTTCAGGCAATCAGGAGCAGGAGCATCTTCAAGCTACGCCAGGGCTTCAAGCCTCAGTGCAAGGCCTTCTTCTCTGGTGGGCTCCATTACAACTACTGCCGGTGCCCTTATCTGCAGGAAGGGATCCCGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

406

Amino Acids

44.55

Weight (kDa)

6.84

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27400 AT3G27400
fragaria_vesca FvH4_6g52190
malus_domestica MD09G1019900.v1.1
prunus_persica Prupe.3G296600_v2.0.a1
pyrus_communis pycom111g01650
rosa_chinensis RchiOBHm_Chr2g0173681
rosa_laevigata RLG00000022214
rosa_multiflora Rmu_sc0002538.1_g000007
rosa_rugosa Rorug02G0572300
rosa_samantha Rh2AG651200 Rh2BG661600 Rh2CG626600 Rh2DG676900
rosa_wichuraiana Rw2G053440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 2 cut(s) 970, 1184
AccI GTMKAC 1 cut(s) 862
AciI CCGC 3 cut(s) 217, 578, 1210
AclWI GGATC 7 cut(s) 191, 349, 699, 712, 1068, 1200, 1213
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 2 cut(s) 258, 971
AdeI CACNNNGTG 1 cut(s) 352
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 3 cut(s) 94, 303, 332
AflIII ACRYGT 1 cut(s) 980
AgsI TTSAA 6 cut(s) 385, 436, 988, 1072, 1113, 1131
AjnI CCWGG 1 cut(s) 1121
AluBI AGCT 4 cut(s) 406, 418, 935, 1116
AluI AGCT 4 cut(s) 406, 418, 935, 1116
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 7 cut(s) 191, 349, 699, 712, 1068, 1200, 1213
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 4 cut(s) 479, 682, 835, 1145
ApeKI GCWGC 3 cut(s) 580, 932, 1212
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 3 cut(s) 225, 239, 973
AspS9I GGNCC 2 cut(s) 479, 652
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 5 cut(s) 286, 481, 709, 895, 1009
AvaII GGWCC 1 cut(s) 652
BaeGI GKGCMC 2 cut(s) 771, 1189
BamHI GGATCC 2 cut(s) 704, 1205
BanI GGYRCC 2 cut(s) 970, 1184
BanII GRGCYC 2 cut(s) 12, 1166
BbvI GCAGC 3 cut(s) 592, 944, 1199
BccI CCATC 8 cut(s) 269, 449, 500, 608, 674, 704, 722, 750
BceAI ACGGC 1 cut(s) 625
BciT130I CCWGG 1 cut(s) 1123
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 2 cut(s) 132, 327
BfmI CTRYAG 1 cut(s) 1195
BfoI RGCGCY 2 cut(s) 226, 974
BfuAI ACCTGC 1 cut(s) 793
BglI GCCNNNNNGGC 1 cut(s) 681
BisI GCNGC 4 cut(s) 578, 581, 933, 1213
BlpI GCTNAGC 1 cut(s) 226
BlsI GCNGC 4 cut(s) 579, 582, 934, 1214
Bme1390I CCNGG 2 cut(s) 333, 1123
Bme18I GGWCC 1 cut(s) 652
BmgT120I GGNCC 2 cut(s) 479, 652
BmiI GGNNCC 6 cut(s) 337, 706, 972, 1165, 1186, 1207
BmrFI CCNGG 2 cut(s) 333, 1123
BmrI ACTGGG 3 cut(s) 214, 311, 913
BmsI GCATC 6 cut(s) 129, 495, 534, 682, 1003, 1115
BmuI ACTGGG 3 cut(s) 214, 311, 913
BplI GAGNNNNNCTC 2 cut(s) 919, 951
BpmI CTGGAG 2 cut(s) 208, 1066
Bpu1102I GCTNAGC 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 2 cut(s) 258, 971
BsaJI CCNNGG 5 cut(s) 520, 700, 854, 952, 1122
BsaWI WCCGGW 1 cut(s) 1018
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 303, 332
Bse118I RCCGGY 1 cut(s) 1181
Bse1I ACTGG 6 cut(s) 99, 169, 209, 317, 908, 1049
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 1 cut(s) 1123
BseDI CCNNGG 5 cut(s) 520, 700, 854, 952, 1122
BseGI GGATG 5 cut(s) 492, 516, 531, 696, 1018
BseLI CCNNNNNNNGG 3 cut(s) 94, 303, 332
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 1 cut(s) 1150
BseNI ACTGG 6 cut(s) 99, 169, 209, 317, 908, 1049
BseRI GAGGAG 2 cut(s) 61, 468
BseSI GKGCMC 2 cut(s) 771, 1189
BseXI GCAGC 3 cut(s) 592, 944, 1199
BseYI CCCAGC 2 cut(s) 5, 551
BsgI GTGCAG 1 cut(s) 951
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 4 cut(s) 481, 684, 837, 1147
BshNI GGYRCC 2 cut(s) 970, 1184
BsiEI CGRYCG 1 cut(s) 280
BsiSI CCGG 4 cut(s) 333, 474, 1019, 1182
BslFI GGGAC 2 cut(s) 320, 404
BslI CCNNNNNNNGG 3 cut(s) 94, 303, 332
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 2 cut(s) 320, 404
BsmI GAATGC 2 cut(s) 583, 855
BsnI GGCC 4 cut(s) 481, 684, 837, 1147
Bsp1286I GDGCHC 4 cut(s) 12, 771, 1166, 1189
Bsp143I GATC 5 cut(s) 196, 354, 704, 1060, 1205
Bsp1720I GCTNAGC 1 cut(s) 226
Bsp19I CCATGG 2 cut(s) 520, 700
BspACI CCGC 3 cut(s) 217, 578, 1210
BspANI GGCC 4 cut(s) 481, 684, 837, 1147
BspCNI CTCAG 1 cut(s) 1149
BspHI TCATGA 2 cut(s) 82, 745
BspLI GGNNCC 6 cut(s) 337, 706, 972, 1165, 1186, 1207
BspMAI CTGCAG 1 cut(s) 1199
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 7 cut(s) 191, 349, 699, 712, 1068, 1200, 1213
BspT107I GGYRCC 2 cut(s) 970, 1184
BsrDI GCAATG 1 cut(s) 655
BsrFI RCCGGY 1 cut(s) 1181
BsrI ACTGG 6 cut(s) 99, 169, 209, 317, 908, 1049
BssAI RCCGGY 1 cut(s) 1181
BssECI CCNNGG 5 cut(s) 520, 700, 854, 952, 1122
BssMI GATC 5 cut(s) 196, 354, 704, 1060, 1205
BssNI GRCGYC 2 cut(s) 258, 971
BssT1I CCWWGG 4 cut(s) 520, 700, 854, 952
Bst2UI CCWGG 1 cut(s) 1123
Bst4CI ACNGT 4 cut(s) 278, 449, 542, 811
BstACI GRCGYC 2 cut(s) 258, 971
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 4 cut(s) 221, 460, 549, 800
BstDEI CTNAG 3 cut(s) 226, 341, 1136
BstDSI CCRYGG 2 cut(s) 520, 700
BstF5I GGATG 5 cut(s) 492, 516, 531, 696, 1018
BstH2I RGCGCY 2 cut(s) 226, 974
BstHHI GCGC 3 cut(s) 225, 239, 973
BstKTI GATC 5 cut(s) 199, 357, 707, 1063, 1208
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 5 cut(s) 196, 354, 704, 1060, 1205
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 62, 236, 681, 766
BstNI CCWGG 1 cut(s) 1123
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 2 cut(s) 331, 1121
BstSFI CTRYAG 1 cut(s) 1195
BstSLI GKGCMC 2 cut(s) 771, 1189
BstSNI TACGTA 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 592, 944, 1199
BstX2I RGATCY 3 cut(s) 704, 1060, 1205
BstYI RGATCY 3 cut(s) 704, 1060, 1205
BsuRI GGCC 4 cut(s) 481, 684, 837, 1147
BtgI CCRYGG 2 cut(s) 520, 700
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 5 cut(s) 492, 516, 531, 696, 1018
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 4 cut(s) 324, 357, 901, 1144
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 4 cut(s) 221, 460, 549, 800
CciI TCATGA 2 cut(s) 82, 745
CfoI GCGC 3 cut(s) 225, 239, 973
Cfr10I RCCGGY 1 cut(s) 1181
Cfr13I GGNCC 2 cut(s) 479, 652
CseI GACGC 2 cut(s) 266, 451
DdeI CTNAG 3 cut(s) 226, 341, 1136
DinI GGCGCC 1 cut(s) 972
DpnI GATC 5 cut(s) 198, 356, 706, 1062, 1207
DpnII GATC 5 cut(s) 196, 354, 704, 1060, 1205
DraIII CACNNNGTG 1 cut(s) 352
Eco105I TACGTA 1 cut(s) 289
Eco130I CCWWGG 4 cut(s) 520, 700, 854, 952
Eco147I AGGCCT 2 cut(s) 837, 1147
Eco24I GRGCYC 2 cut(s) 12, 1166
Eco47I GGWCC 1 cut(s) 652
Eco47III AGCGCT 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 1121
EcoT14I CCWWGG 4 cut(s) 520, 700, 854, 952
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 2 cut(s) 12, 1166
EgeI GGCGCC 1 cut(s) 972
EheI GGCGCC 1 cut(s) 972
ErhI CCWWGG 4 cut(s) 520, 700, 854, 952
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 2 cut(s) 320, 404
FblI GTMKAC 1 cut(s) 862
Fnu4HI GCNGC 4 cut(s) 578, 581, 933, 1213
FokI GGATG 5 cut(s) 479, 503, 518, 683, 1025
FriOI GRGCYC 2 cut(s) 12, 1166
Fsp4HI GCNGC 4 cut(s) 578, 581, 933, 1213
FspBI CTAG 2 cut(s) 132, 327
GlaI GCGC 3 cut(s) 224, 238, 972
GluI GCNGC 4 cut(s) 578, 581, 933, 1213
GsaI CCCAGC 2 cut(s) 9, 555
GsuI CTGGAG 2 cut(s) 208, 1066
HaeII RGCGCY 2 cut(s) 226, 974
HaeIII GGCC 4 cut(s) 481, 684, 837, 1147
HapII CCGG 4 cut(s) 333, 474, 1019, 1182
HgaI GACGC 2 cut(s) 266, 451
HhaI GCGC 3 cut(s) 225, 239, 973
Hin1I GRCGYC 2 cut(s) 258, 971
Hin6I GCGC 3 cut(s) 223, 237, 971
HinP1I GCGC 3 cut(s) 223, 237, 971
HinfI GANTC 4 cut(s) 282, 299, 572, 776
HpaII CCGG 4 cut(s) 333, 474, 1019, 1182
HphI GGTGA 5 cut(s) 286, 481, 709, 895, 1009
Hpy166II GTNNAC 6 cut(s) 322, 466, 597, 652, 863, 886
Hpy188I TCNGA 4 cut(s) 266, 607, 1054, 1065
Hpy188III TCNNGA 4 cut(s) 83, 436, 746, 1095
Hpy8I GTNNAC 6 cut(s) 322, 466, 597, 652, 863, 886
Hpy99I CGWCG 2 cut(s) 314, 449
HpyAV CCTTC 3 cut(s) 827, 1158, 1195
HpyCH4III ACNGT 4 cut(s) 278, 449, 542, 811
HpyCH4IV ACGT 2 cut(s) 288, 982
HpyCH4V TGCA 8 cut(s) 56, 486, 802, 917, 932, 1016, 1142, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 236, 681, 766
HpyF3I CTNAG 3 cut(s) 226, 341, 1136
HpySE526I ACGT 2 cut(s) 288, 982
Hsp92I GRCGYC 2 cut(s) 258, 971
HspAI GCGC 3 cut(s) 223, 237, 971
KasI GGCGCC 1 cut(s) 970
Kzo9I GATC 5 cut(s) 196, 354, 704, 1060, 1205
LmnI GCTCC 6 cut(s) 117, 455, 940, 1097, 1103, 1169
Lsp1109I GCAGC 3 cut(s) 592, 944, 1199
LweI GCATC 6 cut(s) 129, 495, 534, 682, 1003, 1115
MaeI CTAG 2 cut(s) 132, 327
MaeII ACGT 2 cut(s) 288, 982
MaeIII GTNAC 3 cut(s) 292, 805, 997
MalI GATC 5 cut(s) 198, 356, 706, 1062, 1207
MboI GATC 5 cut(s) 196, 354, 704, 1060, 1205
MboII GAAGA 3 cut(s) 282, 1101, 1143
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 3 cut(s) 704, 1060, 1205
MhlI GDGCHC 4 cut(s) 12, 771, 1166, 1189
MluCI AATT 2 cut(s) 670, 918
Mly113I GGCGCC 1 cut(s) 971
MlyI GAGTC 2 cut(s) 293, 566
MmeI TCCRAC 2 cut(s) 962, 1019
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 1011
MspA1I CMGCKG 2 cut(s) 406, 1212
MspI CCGG 4 cut(s) 333, 474, 1019, 1182
MspR9I CCNGG 2 cut(s) 333, 1123
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 2 cut(s) 583, 855
MvaI CCWGG 1 cut(s) 1123
MwoI GCNNNNNNNGC 4 cut(s) 62, 236, 681, 766
NarI GGCGCC 1 cut(s) 971
NciI CCSGG 1 cut(s) 333
NcoI CCATGG 2 cut(s) 520, 700
NdeII GATC 5 cut(s) 196, 354, 704, 1060, 1205
NlaIV GGNNCC 6 cut(s) 337, 706, 972, 1165, 1186, 1207
NmuCI GTSAC 3 cut(s) 292, 805, 997
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 1 cut(s) 802
PaeI GCATGC 1 cut(s) 802
PagI TCATGA 2 cut(s) 82, 745
PceI AGGCCT 2 cut(s) 837, 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 2 cut(s) 583, 855
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 282, 776
PkrI GCNGC 4 cut(s) 579, 582, 934, 1214
PleI GAGTC 2 cut(s) 293, 566
PluTI GGCGCC 1 cut(s) 974
PpsI GAGTC 2 cut(s) 293, 566
Ppu21I YACGTR 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 1121
PspFI CCCAGC 2 cut(s) 5, 551
PspGI CCWGG 1 cut(s) 1121
PspN4I GGNNCC 6 cut(s) 337, 706, 972, 1165, 1186, 1207
PspPI GGNCC 2 cut(s) 479, 652
PsrI GAACNNNNNNTAC 4 cut(s) 131, 163, 878, 910
PstI CTGCAG 1 cut(s) 1199
PsuI RGATCY 3 cut(s) 704, 1060, 1205
PvuII CAGCTG 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 1011
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 4 cut(s) 578, 581, 933, 1213
Sau3AI GATC 5 cut(s) 196, 354, 704, 1060, 1205
Sau96I GGNCC 2 cut(s) 479, 652
SchI GAGTC 2 cut(s) 293, 566
ScrFI CCNGG 2 cut(s) 333, 1123
SduI GDGCHC 4 cut(s) 12, 771, 1166, 1189
SfaNI GCATC 6 cut(s) 129, 495, 534, 682, 1003, 1115
SfcI CTRYAG 1 cut(s) 1195
SfoI GGCGCC 1 cut(s) 972
SinI GGWCC 1 cut(s) 652
SmiMI CAYNNNNRTG 1 cut(s) 1011
SnaBI TACGTA 1 cut(s) 289
SphI GCATGC 1 cut(s) 802
Sse9I AATT 2 cut(s) 670, 918
SseBI AGGCCT 2 cut(s) 837, 1147
SsiI CCGC 3 cut(s) 217, 578, 1210
SspDI GGCGCC 1 cut(s) 970
SspMI CTAG 2 cut(s) 132, 327
StuI AGGCCT 2 cut(s) 837, 1147
StyD4I CCNGG 2 cut(s) 331, 1121
StyI CCWWGG 4 cut(s) 520, 700, 854, 952
TaaI ACNGT 4 cut(s) 278, 449, 542, 811
TaiI ACGT 2 cut(s) 291, 985
TaqI TCGA 2 cut(s) 280, 444
TasI AATT 2 cut(s) 670, 918
TauI GCSGC 1 cut(s) 580
TfiI GAWTC 2 cut(s) 282, 776
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 4 cut(s) 324, 357, 908, 1144
TseFI GTSAC 3 cut(s) 292, 805, 997
TseI GCWGC 3 cut(s) 580, 932, 1212
Tsp45I GTSAC 3 cut(s) 292, 805, 997
TspDTI ATGAA 3 cut(s) 71, 440, 866
TspRI CASTG 4 cut(s) 324, 357, 908, 1144
VpaK11BI GGWCC 1 cut(s) 652
XceI RCATGY 1 cut(s) 802
XmiI GTMKAC 1 cut(s) 862
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 2 cut(s) 132, 327
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.