Rh2BG661600

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015791
Physical Location & Seq
Reverse (-)
87653588 .. 87655699
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG661600.1

Sequence Viewer

Length: 1218 bp
ATGGAAACCCAGCTCTCAATGTTGGTTATTCTCCTCGCTTCTTTTCTAACTGCAACTACTTTTGTTTCCTGCTCCCAACTTCAAGACCCTGATCTAGTAGTACAGGAGGTCCACAGGAATATCAATGCGTCTAGGAGGCAGCTGGCCTATTCCTCATGTGGAAGTGGCAACCCCATAGACGACTGCTGGCGGTGCGACCCTAACTGGGAACAAAACCGCCAGCGCTTGGCCGATTGCGCCATCGGCTTCGGCAAGGACGCAATTGGCGGGAGAAACGGCCGAATCTACGTAGTCTCCGACTCAGGAGACGACGACCCTGTGAACCCTAGGCCCGGAACCATCCGACACGCCGTGATACAAGACGAGCCACTGTGGATCATTTTCAAGAGGGACATGGTGATCCAGCTGAAGCAAGAGCTGGTCATGAACTCGTTCAAGACCATAGACGGTAGAGGTGCGAGTGTTCACATTGCCAATGGACCATGCATCACCATACATCATGTCACCAACATCATCATCCATGGGATCAACATACACGACTGTAAGCAGGCCGGGAACGGAGACATTAGGGACTCCCCAAGCCACGCCGGGTGGTGGACGCAGTCCGACGGGGACGGCGTGTCGATTTTCGGCGGAAAGCACATTTGGGTTGACCATTGCTCGCTGTCAAATTGTCACGATGGACTCATCGATGCCATACATGGATCGACGGCCATCACGATTTCGAACAACTTCATGACGCATCATGACAAGGTTATGCTTTTAGGGCACAGTGATTCGTTTACGGAAGACAAGGGCATGCAGGTCACTGTTGCGTTTAATCACTTTGGAGAAGGGCTCGTGCAAAGAATGCCAAGGTGTAGACATGGATATTTTCATGTGGTGAACAATGACTATACACATTGGGAGATGTATGCAATAGGAGGGAGTGCTGCCCCAACCATCTTAAGTCAAGGCAATAGATTTCTTGCACCGGATAGAAGTTTCGATAAAGAGGTGACAAAACATGAGGATGCGCCGGAAAGTGAGTGGAAGAAGTGGAACTGGAGATCCGAGGGGGATTTGATGCTGAACGGCGCATTTTTCCGGCAATCGGGTGCTGGTGCATCGTCAAGCTATGCGAGGGCGTCGAGTCTCAGTGGAAGACCTTCTTCTCTTGTGGGTGCAATGACTGTCACTGCCGGTGCACTTAACTGCCGGAGAGGATCCCGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

405

Amino Acids

44.66

Weight (kDa)

6.36

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 137 - 326 3.4e-23 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27400 AT3G27400
fragaria_vesca FvH4_6g52190
malus_domestica MD09G1019900.v1.1
prunus_persica Prupe.3G296600_v2.0.a1
pyrus_communis pycom111g01650
rosa_chinensis RchiOBHm_Chr2g0173681
rosa_laevigata RLG00000022214
rosa_multiflora Rmu_sc0002538.1_g000007
rosa_rugosa Rorug02G0572300
rosa_samantha Rh2AG651200 Rh2BG661600 Rh2CG626600 Rh2DG676900
rosa_wichuraiana Rw2G053440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 793
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 1 cut(s) 862
AciI CCGC 5 cut(s) 190, 217, 267, 633, 1210
AclWI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1200, 1213
AcoI YGGCCR 3 cut(s) 228, 277, 711
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 1127
AdeI CACNNNGTG 1 cut(s) 352
AfaI GTAC 1 cut(s) 102
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 5 cut(s) 205, 226, 332, 594, 1093
AflII CTTAAG 1 cut(s) 946
AgsI TTSAA 3 cut(s) 83, 385, 436
AhdI GACNNNNNGTC 1 cut(s) 619
AjuI GAANNNNNNNTTGG 2 cut(s) 628, 660
AloI GAACNNNNNNTCC 2 cut(s) 1034, 1066
AluBI AGCT 5 cut(s) 13, 142, 406, 418, 1116
AluI AGCT 5 cut(s) 13, 142, 406, 418, 1116
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 4 cut(s) 298, 300, 555, 1139
Alw44I GTGCAC 1 cut(s) 1185
AlwI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1200, 1213
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 6 cut(s) 144, 228, 277, 329, 549, 711
ApaLI GTGCAC 1 cut(s) 1185
ApeKI GCWGC 3 cut(s) 139, 932, 1212
Asp700I GAANNNNTTC 3 cut(s) 431, 731, 1147
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 4 cut(s) 225, 239, 1018, 1079
AspS9I GGNCC 3 cut(s) 109, 330, 479
AsuC2I CCSGG 3 cut(s) 333, 553, 589
AsuHPI GGTGA 5 cut(s) 409, 481, 496, 895, 1009
AsuII TTCGAA 1 cut(s) 725
AvaII GGWCC 2 cut(s) 109, 479
AvrII CCTAGG 1 cut(s) 326
BaeGI GKGCMC 2 cut(s) 771, 1189
BamHI GGATCC 1 cut(s) 1205
BanII GRGCYC 1 cut(s) 840
BauI CACGAG 1 cut(s) 839
BbsI GAAGAC 2 cut(s) 795, 1150
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 3 cut(s) 151, 919, 1199
BccI CCATC 5 cut(s) 248, 347, 674, 722, 950
BceAI ACGGC 5 cut(s) 292, 335, 631, 726, 1090
BcnI CCSGG 3 cut(s) 333, 553, 589
BcoDI GTCTC 4 cut(s) 298, 300, 555, 1139
BfaI CTAG 3 cut(s) 95, 132, 327
BfoI RGCGCY 1 cut(s) 226
BfrI CTTAAG 1 cut(s) 946
BfuAI ACCTGC 1 cut(s) 793
BisI GCNGC 3 cut(s) 140, 933, 1213
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 3 cut(s) 141, 934, 1214
Bme1390I CCNGG 3 cut(s) 333, 553, 589
Bme18I GGWCC 2 cut(s) 109, 479
BmeRI GACNNNNNGTC 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 109, 330, 479
BmiI GGNNCC 2 cut(s) 337, 1207
BmrFI CCNGG 3 cut(s) 333, 553, 589
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 6 cut(s) 495, 682, 751, 1003, 1056, 1115
BmuI ACTGGG 1 cut(s) 214
BpiI GAAGAC 2 cut(s) 795, 1150
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 1 cut(s) 1066
Bpu14I TTCGAA 1 cut(s) 725
BpuMI CCSGG 3 cut(s) 333, 553, 589
Bsa29I ATCGAT 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 1127
BsaJI CCNNGG 4 cut(s) 326, 520, 854, 1053
BsaWI WCCGGW 1 cut(s) 973
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 226, 332, 594, 1093
Bse118I RCCGGY 1 cut(s) 1181
Bse1I ACTGG 2 cut(s) 209, 1049
Bse3DI GCAATG 3 cut(s) 468, 655, 1173
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 4 cut(s) 326, 520, 854, 1053
BseGI GGATG 3 cut(s) 339, 516, 1018
BseLI CCNNNNNNNGG 5 cut(s) 205, 226, 332, 594, 1093
BseMI GCAATG 3 cut(s) 468, 655, 1173
BseMII CTCAG 2 cut(s) 315, 1150
BseNI ACTGG 2 cut(s) 209, 1049
BseRI GAGGAG 1 cut(s) 23
BseSI GKGCMC 2 cut(s) 771, 1189
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 3 cut(s) 151, 919, 1199
BseYI CCCAGC 1 cut(s) 9
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 6 cut(s) 146, 230, 279, 331, 551, 713
BshVI ATCGAT 1 cut(s) 690
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 1189
BsiSI CCGG 8 cut(s) 333, 552, 588, 974, 1019, 1087, 1182, 1198
BslFI GGGAC 3 cut(s) 404, 584, 626
BslI CCNNNNNNNGG 5 cut(s) 205, 226, 332, 594, 1093
BsmAI GTCTC 4 cut(s) 298, 300, 555, 1139
BsmBI CGTCTC 1 cut(s) 300
BsmFI GGGAC 3 cut(s) 404, 584, 626
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 6 cut(s) 146, 230, 279, 331, 551, 713
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 3 cut(s) 771, 840, 1189
Bsp143I GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 5 cut(s) 190, 217, 267, 633, 1210
BspANI GGCC 6 cut(s) 146, 230, 279, 331, 551, 713
BspCNI CTCAG 2 cut(s) 314, 1149
BspDI ATCGAT 1 cut(s) 690
BspHI TCATGA 3 cut(s) 423, 735, 745
BspLI GGNNCC 2 cut(s) 337, 1207
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1200, 1213
BspT104I TTCGAA 1 cut(s) 725
BspTI CTTAAG 1 cut(s) 946
BsrDI GCAATG 3 cut(s) 468, 655, 1173
BsrFI RCCGGY 1 cut(s) 1181
BsrI ACTGG 2 cut(s) 209, 1049
BssAI RCCGGY 1 cut(s) 1181
BssECI CCNNGG 4 cut(s) 326, 520, 854, 1053
BssMI GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
BssNI GRCGYC 1 cut(s) 1127
BssSI CACGAG 1 cut(s) 839
BssT1I CCWWGG 3 cut(s) 326, 520, 854
Bst2BI CACGAG 1 cut(s) 839
Bst4CI ACNGT 6 cut(s) 372, 449, 542, 773, 811, 1174
BstACI GRCGYC 1 cut(s) 1127
BstAFI CTTAAG 1 cut(s) 946
BstAPI GCANNNNNTGC 1 cut(s) 850
BstBAI YACGTR 1 cut(s) 289
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 6 cut(s) 144, 188, 221, 549, 662, 800
BstDEI CTNAG 2 cut(s) 301, 1136
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 3 cut(s) 339, 516, 1018
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 4 cut(s) 225, 239, 1018, 1079
BstKTI GATC 7 cut(s) 94, 378, 402, 528, 707, 1052, 1208
BstMAI GTCTC 4 cut(s) 298, 300, 555, 1139
BstMBI GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 5 cut(s) 192, 236, 243, 766, 850
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 3 cut(s) 331, 551, 587
BstSLI GKGCMC 2 cut(s) 771, 1189
BstSNI TACGTA 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 151, 919, 1199
BstV2I GAAGAC 2 cut(s) 795, 1150
BstX2I RGATCY 2 cut(s) 1049, 1205
BstYI RGATCY 2 cut(s) 1049, 1205
BstZI CGGCCG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 690
BsuRI GGCC 6 cut(s) 146, 230, 279, 331, 551, 713
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 3 cut(s) 339, 516, 1018
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 5 cut(s) 368, 778, 807, 1144, 1176
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 6 cut(s) 144, 188, 221, 549, 662, 800
CciI TCATGA 3 cut(s) 423, 735, 745
CfoI GCGC 4 cut(s) 225, 239, 1018, 1079
Cfr10I RCCGGY 1 cut(s) 1181
Cfr13I GGNCC 3 cut(s) 109, 330, 479
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 5 cut(s) 117, 266, 607, 748, 1116
Csp6I GTAC 1 cut(s) 101
CviQI GTAC 1 cut(s) 101
DdeI CTNAG 2 cut(s) 301, 1136
DpnI GATC 7 cut(s) 93, 377, 401, 527, 706, 1051, 1207
DpnII GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
DraIII CACNNNGTG 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 3 cut(s) 228, 277, 711
EagI CGGCCG 1 cut(s) 277
Eam1105I GACNNNNNGTC 1 cut(s) 619
EciI GGCGGA 1 cut(s) 648
EclXI CGGCCG 1 cut(s) 277
Eco105I TACGTA 1 cut(s) 289
Eco130I CCWWGG 3 cut(s) 326, 520, 854
Eco24I GRGCYC 1 cut(s) 840
Eco47I GGWCC 2 cut(s) 109, 479
Eco47III AGCGCT 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 428
EcoT14I CCWWGG 3 cut(s) 326, 520, 854
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 3 cut(s) 326, 520, 854
Esp3I CGTCTC 1 cut(s) 300
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 3 cut(s) 404, 584, 626
FauI CCCGC 1 cut(s) 260
FblI GTMKAC 1 cut(s) 862
Fnu4HI GCNGC 3 cut(s) 140, 933, 1213
FokI GGATG 3 cut(s) 326, 503, 1025
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 3 cut(s) 140, 933, 1213
FspBI CTAG 3 cut(s) 95, 132, 327
GlaI GCGC 4 cut(s) 224, 238, 1017, 1078
GluI GCNGC 3 cut(s) 140, 933, 1213
GsaI CCCAGC 1 cut(s) 13
GsuI CTGGAG 1 cut(s) 1066
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 6 cut(s) 146, 230, 279, 331, 551, 713
HapII CCGG 8 cut(s) 333, 552, 588, 974, 1019, 1087, 1182, 1198
HgaI GACGC 5 cut(s) 117, 266, 607, 748, 1116
HhaI GCGC 4 cut(s) 225, 239, 1018, 1079
Hin1I GRCGYC 1 cut(s) 1127
Hin6I GCGC 4 cut(s) 223, 237, 1016, 1077
HinP1I GCGC 4 cut(s) 223, 237, 1016, 1077
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 6 cut(s) 282, 299, 572, 684, 776, 1132
HpaII CCGG 8 cut(s) 333, 552, 588, 974, 1019, 1087, 1182, 1198
HphI GGTGA 5 cut(s) 409, 481, 496, 895, 1009
Hpy166II GTNNAC 9 cut(s) 112, 322, 466, 597, 652, 783, 863, 886, 1187
Hpy188I TCNGA 4 cut(s) 298, 344, 607, 1054
Hpy188III TCNNGA 9 cut(s) 83, 303, 385, 424, 436, 677, 718, 736, 746
Hpy8I GTNNAC 9 cut(s) 112, 322, 466, 597, 652, 783, 863, 886, 1187
Hpy99I CGWCG 4 cut(s) 314, 611, 712, 1132
HpyAV CCTTC 2 cut(s) 827, 1158
HpyCH4III ACNGT 6 cut(s) 372, 449, 542, 773, 811, 1174
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 9 cut(s) 53, 486, 802, 844, 917, 971, 1106, 1166, 1187
HpyF10VI GCNNNNNNNGC 5 cut(s) 192, 236, 243, 766, 850
HpyF3I CTNAG 2 cut(s) 301, 1136
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 1 cut(s) 1127
HspAI GCGC 4 cut(s) 223, 237, 1016, 1077
Kzo9I GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 3 cut(s) 151, 919, 1199
LweI GCATC 6 cut(s) 495, 682, 751, 1003, 1056, 1115
MaeI CTAG 3 cut(s) 95, 132, 327
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 5 cut(s) 502, 674, 805, 997, 1174
MalI GATC 7 cut(s) 93, 377, 401, 527, 706, 1051, 1207
MboI GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
MboII GAAGA 4 cut(s) 800, 1045, 1143, 1155
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 2 cut(s) 1049, 1205
MhlI GDGCHC 3 cut(s) 771, 840, 1189
MluCI AATT 2 cut(s) 261, 670
MlyI GAGTC 4 cut(s) 293, 566, 678, 1141
MmeI TCCRAC 3 cut(s) 321, 367, 630
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 3 cut(s) 431, 731, 1147
MseI TTAA 3 cut(s) 819, 947, 1191
MslI CAYNNNNRTG 1 cut(s) 1011
MspA1I CMGCKG 3 cut(s) 142, 406, 1212
MspCI CTTAAG 1 cut(s) 946
MspI CCGG 8 cut(s) 333, 552, 588, 974, 1019, 1087, 1182, 1198
MspR9I CCNGG 3 cut(s) 333, 553, 589
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 855
MwoI GCNNNNNNNGC 5 cut(s) 192, 236, 243, 766, 850
NciI CCSGG 3 cut(s) 333, 553, 589
NcoI CCATGG 1 cut(s) 520
NdeII GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
NlaIV GGNNCC 2 cut(s) 337, 1207
NmuCI GTSAC 5 cut(s) 502, 674, 805, 997, 1174
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 725
PaeI GCATGC 1 cut(s) 802
PagI TCATGA 3 cut(s) 423, 735, 745
PcsI WCGNNNNNNNCGW 3 cut(s) 294, 615, 716
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 3 cut(s) 431, 731, 1147
PfeI GAWTC 2 cut(s) 282, 776
PflFI GACNNNGTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 3 cut(s) 141, 934, 1214
PleI GAGTC 4 cut(s) 293, 566, 678, 1140
PpsI GAGTC 4 cut(s) 293, 566, 678, 1140
Ppu21I YACGTR 1 cut(s) 289
PspFI CCCAGC 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 337, 1207
PspPI GGNCC 3 cut(s) 109, 330, 479
PsuI RGATCY 2 cut(s) 1049, 1205
PsyI GACNNNGTC 1 cut(s) 601
PvuII CAGCTG 2 cut(s) 142, 406
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 1 cut(s) 1011
SaqAI TTAA 3 cut(s) 819, 947, 1191
SatI GCNGC 3 cut(s) 140, 933, 1213
Sau3AI GATC 7 cut(s) 91, 375, 399, 525, 704, 1049, 1205
Sau96I GGNCC 3 cut(s) 109, 330, 479
SchI GAGTC 4 cut(s) 293, 566, 678, 1141
ScrFI CCNGG 3 cut(s) 333, 553, 589
SduI GDGCHC 3 cut(s) 771, 840, 1189
SfaNI GCATC 6 cut(s) 495, 682, 751, 1003, 1056, 1115
SfuI TTCGAA 1 cut(s) 725
SinI GGWCC 2 cut(s) 109, 479
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 1 cut(s) 946
SmoI CTYRAG 1 cut(s) 946
SnaBI TACGTA 1 cut(s) 289
SphI GCATGC 1 cut(s) 802
Sse9I AATT 2 cut(s) 261, 670
SsiI CCGC 5 cut(s) 190, 217, 267, 633, 1210
SspMI CTAG 3 cut(s) 95, 132, 327
StyD4I CCNGG 3 cut(s) 331, 551, 587
StyI CCWWGG 3 cut(s) 326, 520, 854
TaaI ACNGT 6 cut(s) 372, 449, 542, 773, 811, 1174
TaiI ACGT 1 cut(s) 291
TaqI TCGA 6 cut(s) 623, 690, 707, 725, 987, 1130
TasI AATT 2 cut(s) 261, 670
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 2 cut(s) 282, 776
Tru1I TTAA 3 cut(s) 819, 947, 1191
Tru9I TTAA 3 cut(s) 819, 947, 1191
TscAI CASTG 5 cut(s) 375, 778, 814, 1144, 1183
TseFI GTSAC 5 cut(s) 502, 674, 805, 997, 1174
TseI GCWGC 3 cut(s) 139, 932, 1212
Tsp45I GTSAC 5 cut(s) 502, 674, 805, 997, 1174
TspDTI ATGAA 3 cut(s) 440, 724, 866
TspGWI ACGGA 2 cut(s) 573, 800
TspRI CASTG 5 cut(s) 375, 778, 814, 1144, 1183
Tth111I GACNNNGTC 1 cut(s) 601
Van91I CCANNNNNTGG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 946
VneI GTGCAC 1 cut(s) 1185
VpaK11BI GGWCC 2 cut(s) 109, 479
XceI RCATGY 1 cut(s) 802
XmaJI CCTAGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 862
XmnI GAANNNNTTC 3 cut(s) 431, 731, 1147
XspI CTAG 3 cut(s) 95, 132, 327
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.