RLG00000022214

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015791
Physical Location & Seq
Reverse (-)
81729127 .. 81732626
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022214

Sequence Viewer

Length: 2262 bp
ATGGAAACCCAGCTCTCAATGTTGGTTATTCTCCTCGCTTCTTTTCTAACTGCAACTACTTTTGTTTCCTGCTCCCAACTTCAAGACCCTGATCTAGTAGTACAGGAGGTCCACAGGAATATCAATGCGTCTAGGAGGCAGCTGGCCTATTCCTCATGTGGAAATGGCAACCCCATAGACGACTGCTGGCGGTGCGACCCTAACTGGGAACAAAACCGCCAGCGACTGGCCGATTGCGCCATCGGCTTCGGCAAGGACGCAATTGGAGGGAGAAACGGCCGCATTTATGTAGTCTCCGACTCAGGCGACGACGACCCTATGAACCCTAGGCCCGGAACCATCCGACACGCCGTGATACAAGATGAGCCACTGTGGATCGCTTTCAAGAGGGACATGGTGATCCAGCTGAAGCAAGAGCTGGTCATGAACTCGTTCAAGACCATAGACGGTAGAGGTGCGAGTGTTCACATTGCCAATGGACCATGCATCACCATACATCACGTCACCAACATCATCATCCATGGGATCAACATACACGACTGTAAGCAGGCCGGGAACGGAGACATTAGGGACTCCCCGAGCCATGCCGGGTGGTGGACGCAGTCCGATGGGGACGGCGTGTCGATTTTCGGCGGGAAGCACATTTGGGTTGACCATTGCTCGCTGTCAAATTGTCATGATGGACTCATCGATGCCATACATGGATCGACGGCCATCACGATTTCGAACAACTTCATGACGCATCATGACAAGGTTATGCTTTTAGGGCACAGTGATTCGTTTACGGAAGACAAGGGCATGCAGGTCACTGTTGCGTTTAATCACTTTGGAGAAGGGCTCGTGCAAAGAATGCCAAGGTGTAGACATGGATATTTTCATGTGGTGAACAATGACTATACACATTGGGAGATGTATGCAATAGGAGGGAGTGCTGCCCCAACCATCTTAAGTCAAGGCAATAGATTTCTTGCACCGGATAGAAGTTTCGATAAAGAGGTGACAAAACATGAGGATGCGCCGGAAAGTGAGTGGAAGAATTGGAACTGGAGATCCGAGGGGGATTTGATGCTGAACGGCGCATTTTTCCGGCAATCGGGTGCGGGTGCATCGTCAAGCTATGCGAGGGCGTCGAGTCTCAGTGGAAGACCTTCTTCTCTTGTGGGTGCGATGACTGTCACTGCCGAAACGAAAACTAACTTAAAAACTCAGAAAGCAAAGAAGCCAGCGACCACTACGATAGCCGCGGCGGCGCCAGAGGTGGAGACCTTTCTCGCAGCAGCAGATTTCGGAGCCTCACTGGGAGGAACAACAGGGGAATCACTACCGGTAGTGGTGTCATCGGTGGGGACGGGGGGAGATGTTGCAGCCGATGAGTCTGGTACCGGTGCGGAGGAGGTTGAAGAGGAGTCGTCGGAGCTGGATGATGATGACCCCTTGGGTGGCTTGCTGCTGCTGCCTGAAGATGAAGATGATGAGTCAGATGAATCAGATGATGAAGATCCTGACCCCTTGGGTGACGATGCTTTGCCGCCGCTACCGCCAGAGGGAGTTGGTGCTTTAGCGCTGCCGCTACTGCTACCGCCAGAGGAAGATCCTGACCCCTTGGGTGGTGATGCTTTGCCTCCACCGCTGCCGCCGCCGCTGCTACCGCCATCGGACGAGTCGGATGAAGGTCCCGGAGACGCTTTAGGTGAAGGAGACTGTTTGGGGGATGGTGCTGATTTGCCGCTTGAGCCGCCGGAAGAAGAAGAGTCCGGTGCGTTGGCGGGAGATGCCTTAGGAGATGGTGAAGAAGTGGGAGAGGATGAGGAGCCCCCCTTGTCGGACTTGGGTGACGGTGCCGATGAAGACGAGTCCGAGCCCTTCGGTGATGATGCTGATGATGATGAGGAGGAGGAGGATGAGTCTTTCGCCTTAGGGGCATCGGCCGATGATGATGAAGAGGACGAGTCTTTCGCCTTGGGGGCATCGGCCGATGATGATGAGGAGGACGAATCTTTCGGGTTAGGGGGTGATGCTGATGGAGAGCCGTCGTCGCCGTCGCTTTCCGTGTCTTTTTTTGGATTCTTTGAGGCCTTGGAGGGTGCTGGTGACTCCGCCATGGAAACTCCGATAATGGCAACCAACATGAGAGCGAGAATGATCACTTTCCTACTCTCCATTTTGGCCATTGTCAGAAAGACTGCGCGGCCAGAGGATGAGGGATATGGTGGTGAAGGGGATGAGATTTATGGGGCTCGACTTGGAAGAGAGGTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

754

Amino Acids

80.24

Weight (kDa)

4.11

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 137 - 335 7e-23 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27400 AT3G27400
fragaria_vesca FvH4_6g52190
malus_domestica MD09G1019900.v1.1
prunus_persica Prupe.3G296600_v2.0.a1
pyrus_communis pycom111g01650
rosa_chinensis RchiOBHm_Chr2g0173681
rosa_laevigata RLG00000022214
rosa_multiflora Rmu_sc0002538.1_g000007
rosa_rugosa Rorug02G0572300
rosa_samantha Rh2AG651200 Rh2BG661600 Rh2CG626600 Rh2DG676900
rosa_wichuraiana Rw2G053440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 793
Acc65I GGTACC 1 cut(s) 1379
AccB1I GGYRCC 3 cut(s) 1249, 1379, 1838
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 2 cut(s) 862, 2256
AccII CGCG 2 cut(s) 1244, 2188
AclWI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1493, 1586
AcoI YGGCCR 7 cut(s) 228, 277, 711, 1926, 1971, 2166, 2189
AcuI CTGAAG 2 cut(s) 428, 1479
AcyI GRCGYC 2 cut(s) 1127, 1250
AdeI CACNNNGTG 1 cut(s) 352
AfaI GTAC 2 cut(s) 102, 1381
AfeI AGCGCT 1 cut(s) 1563
AfiI CCNNNNNNNGG 9 cut(s) 205, 226, 332, 594, 1093, 1439, 1544, 1607, 1822
AflII CTTAAG 1 cut(s) 946
AgeI ACCGGT 2 cut(s) 1324, 1382
AgsI TTSAA 4 cut(s) 83, 385, 436, 1400
AhdI GACNNNNNGTC 1 cut(s) 619
AjiI CACGTC 1 cut(s) 502
AloI GAACNNNNNNTCC 2 cut(s) 1034, 1066
AluBI AGCT 6 cut(s) 13, 142, 406, 418, 1116, 1417
AluI AGCT 6 cut(s) 13, 142, 406, 418, 1116, 1417
Alw26I GTCTC 6 cut(s) 298, 555, 1139, 1256, 1674, 1692
AlwI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1493, 1586
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 1563
ArsI GACNNNNNNTTYG 4 cut(s) 1937, 1969, 1982, 2014
AsiGI ACCGGT 2 cut(s) 1324, 1382
Asp700I GAANNNNTTC 3 cut(s) 431, 731, 1147
Asp718I GGTACC 1 cut(s) 1379
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 6 cut(s) 239, 1018, 1079, 1252, 1564, 2188
AspS9I GGNCC 4 cut(s) 109, 330, 479, 1673
AsuC2I CCSGG 4 cut(s) 333, 553, 589, 1677
AsuII TTCGAA 1 cut(s) 725
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 3 cut(s) 109, 479, 1673
AvrII CCTAGG 1 cut(s) 326
AxyI CCTNAGG 2 cut(s) 1777, 1915
BaeGI GKGCMC 1 cut(s) 771
BalI TGGCCA 1 cut(s) 2168
BanI GGYRCC 3 cut(s) 1249, 1379, 1838
BanII GRGCYC 4 cut(s) 840, 1815, 1863, 2239
BauI CACGAG 1 cut(s) 839
BbsI GAAGAC 3 cut(s) 795, 1150, 1854
BceAI ACGGC 7 cut(s) 292, 335, 631, 726, 1090, 2014, 2023
BclI TGATCA 1 cut(s) 2142
BcnI CCSGG 4 cut(s) 333, 553, 589, 1677
BcoDI GTCTC 6 cut(s) 298, 555, 1139, 1256, 1674, 1692
BfaI CTAG 4 cut(s) 95, 132, 327, 2260
BfoI RGCGCY 2 cut(s) 1253, 1565
BfrI CTTAAG 1 cut(s) 946
BfuAI ACCTGC 1 cut(s) 793
BglI GCCNNNNNGGC 3 cut(s) 1247, 1919, 1964
BlnI CCTAGG 1 cut(s) 326
Bme1390I CCNGG 4 cut(s) 333, 553, 589, 1677
Bme18I GGWCC 3 cut(s) 109, 479, 1673
BmeRI GACNNNNNGTC 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 4 cut(s) 109, 330, 479, 1673
BmiI GGNNCC 7 cut(s) 337, 1251, 1291, 1381, 1675, 1812, 1840
BmrFI CCNGG 4 cut(s) 333, 553, 589, 1677
BmrI ACTGGG 2 cut(s) 214, 1307
BmuI ACTGGG 2 cut(s) 214, 1307
BpiI GAAGAC 3 cut(s) 795, 1150, 1854
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 1 cut(s) 1066
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 1751
BpuMI CCSGG 4 cut(s) 333, 553, 589, 1677
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 1497
BsaHI GRCGYC 2 cut(s) 1127, 1250
BsaI GGTCTC 1 cut(s) 1256
BsaWI WCCGGW 4 cut(s) 973, 1324, 1382, 1754
BsaXI ACNNNNNCTCC 2 cut(s) 1889, 1919
Bsc4I CCNNNNNNNGG 9 cut(s) 205, 226, 332, 594, 1093, 1439, 1544, 1607, 1822
Bse118I RCCGGY 2 cut(s) 1324, 1382
Bse1I ACTGG 4 cut(s) 209, 231, 1049, 1302
Bse21I CCTNAGG 2 cut(s) 1777, 1915
Bse3DI GCAATG 2 cut(s) 468, 655
Bse8I GATNNNNATC 1 cut(s) 1497
BseCI ATCGAT 1 cut(s) 690
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 9 cut(s) 205, 226, 332, 594, 1093, 1439, 1544, 1607, 1822
BseMI GCAATG 2 cut(s) 468, 655
BseMII CTCAG 3 cut(s) 315, 1150, 1220
BseNI ACTGG 4 cut(s) 209, 231, 1049, 1302
BseRI GAGGAG 8 cut(s) 23, 1406, 1418, 1823, 1904, 1907, 1910, 2000
BseSI GKGCMC 1 cut(s) 771
BseX3I CGGCCG 3 cut(s) 277, 1926, 1971
BseYI CCCAGC 1 cut(s) 9
Bsh1236I CGCG 2 cut(s) 1244, 2188
Bsh1285I CGRYCG 3 cut(s) 280, 1929, 1974
BshNI GGYRCC 3 cut(s) 1249, 1379, 1838
BshTI ACCGGT 2 cut(s) 1324, 1382
BshVI ATCGAT 1 cut(s) 690
BsiEI CGRYCG 3 cut(s) 280, 1929, 1974
BsiHKCI CYCGRG 1 cut(s) 577
BslFI GGGAC 5 cut(s) 404, 584, 626, 1360, 1659
BslI CCNNNNNNNGG 9 cut(s) 205, 226, 332, 594, 1093, 1439, 1544, 1607, 1822
BsmAI GTCTC 6 cut(s) 298, 555, 1139, 1256, 1674, 1692
BsmBI CGTCTC 1 cut(s) 1674
BsmFI GGGAC 5 cut(s) 404, 584, 626, 1360, 1659
BsmI GAATGC 1 cut(s) 855
Bso31I GGTCTC 1 cut(s) 1256
BsoBI CYCGRG 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 5 cut(s) 771, 840, 1815, 1863, 2239
Bsp143I GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
Bsp19I CCATGG 2 cut(s) 520, 2100
BspCNI CTCAG 3 cut(s) 314, 1149, 1219
BspDI ATCGAT 1 cut(s) 690
BspFNI CGCG 2 cut(s) 1244, 2188
BspHI TCATGA 4 cut(s) 423, 676, 735, 745
BspLI GGNNCC 7 cut(s) 337, 1251, 1291, 1381, 1675, 1812, 1840
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 7 cut(s) 383, 394, 533, 712, 1044, 1493, 1586
BspT104I TTCGAA 1 cut(s) 725
BspT107I GGYRCC 3 cut(s) 1249, 1379, 1838
BspTI CTTAAG 1 cut(s) 946
BspTNI GGTCTC 1 cut(s) 1256
BsrDI GCAATG 2 cut(s) 468, 655
BsrFI RCCGGY 2 cut(s) 1324, 1382
BsrI ACTGG 4 cut(s) 209, 231, 1049, 1302
BssAI RCCGGY 2 cut(s) 1324, 1382
BssMI GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
BssNI GRCGYC 2 cut(s) 1127, 1250
BssSI CACGAG 1 cut(s) 839
BssT1I CCWWGG 9 cut(s) 326, 520, 854, 1434, 1509, 1602, 1959, 2076, 2100
Bst2BI CACGAG 1 cut(s) 839
Bst4CI ACNGT 8 cut(s) 372, 449, 542, 773, 811, 1174, 1703, 1838
Bst6I CTCTTC 4 cut(s) 1395, 1743, 1935, 2242
BstACI GRCGYC 2 cut(s) 1127, 1250
BstAFI CTTAAG 1 cut(s) 946
BstAPI GCANNNNNTGC 1 cut(s) 850
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 8 cut(s) 144, 188, 221, 549, 662, 800, 1224, 1445
BstDEI CTNAG 5 cut(s) 301, 1136, 1206, 1777, 1915
BstDSI CCRYGG 3 cut(s) 520, 1242, 2100
BstFNI CGCG 2 cut(s) 1244, 2188
BstH2I RGCGCY 2 cut(s) 1253, 1565
BstHHI GCGC 6 cut(s) 239, 1018, 1079, 1252, 1564, 2188
BstKTI GATC 9 cut(s) 94, 378, 402, 528, 707, 1052, 1501, 1594, 2145
BstMAI GTCTC 6 cut(s) 298, 555, 1139, 1256, 1674, 1692
BstMBI GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
BstMCI CGRYCG 3 cut(s) 280, 1929, 1974
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 4 cut(s) 331, 551, 587, 1675
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 2 cut(s) 1244, 2188
BstV2I GAAGAC 3 cut(s) 795, 1150, 1854
BstX2I RGATCY 3 cut(s) 1049, 1498, 1591
BstYI RGATCY 3 cut(s) 1049, 1498, 1591
BstZI CGGCCG 3 cut(s) 277, 1926, 1971
Bsu15I ATCGAT 1 cut(s) 690
Bsu36I CCTNAGG 2 cut(s) 1777, 1915
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 3 cut(s) 520, 1242, 2100
BtgZI GCGATG 1 cut(s) 1181
BtrI CACGTC 1 cut(s) 502
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 6 cut(s) 368, 778, 807, 1144, 1176, 1295
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 8 cut(s) 144, 188, 221, 549, 662, 800, 1224, 1445
CaiI CAGNNNCTG 1 cut(s) 226
CciI TCATGA 4 cut(s) 423, 676, 735, 745
CfoI GCGC 6 cut(s) 239, 1018, 1079, 1252, 1564, 2188
Cfr10I RCCGGY 2 cut(s) 1324, 1382
Cfr13I GGNCC 4 cut(s) 109, 330, 479, 1673
Cfr42I CCGCGG 1 cut(s) 1245
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 6 cut(s) 117, 266, 607, 748, 1116, 1691
Csp6I GTAC 2 cut(s) 101, 1380
CspAI ACCGGT 2 cut(s) 1324, 1382
CviQI GTAC 2 cut(s) 101, 1380
DdeI CTNAG 5 cut(s) 301, 1136, 1206, 1777, 1915
DinI GGCGCC 1 cut(s) 1251
DpnI GATC 9 cut(s) 93, 377, 401, 527, 706, 1051, 1500, 1593, 2144
DpnII GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
DraIII CACNNNGTG 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 7 cut(s) 228, 277, 711, 1926, 1971, 2166, 2189
EagI CGGCCG 3 cut(s) 277, 1926, 1971
Eam1104I CTCTTC 4 cut(s) 1395, 1743, 1935, 2242
Eam1105I GACNNNNNGTC 1 cut(s) 619
EarI CTCTTC 4 cut(s) 1395, 1743, 1935, 2242
EciI GGCGGA 1 cut(s) 2086
EclXI CGGCCG 3 cut(s) 277, 1926, 1971
Eco130I CCWWGG 9 cut(s) 326, 520, 854, 1434, 1509, 1602, 1959, 2076, 2100
Eco147I AGGCCT 1 cut(s) 2075
Eco24I GRGCYC 4 cut(s) 840, 1815, 1863, 2239
Eco31I GGTCTC 1 cut(s) 1256
Eco47I GGWCC 3 cut(s) 109, 479, 1673
Eco47III AGCGCT 1 cut(s) 1563
Eco52I CGGCCG 3 cut(s) 277, 1926, 1971
Eco57I CTGAAG 2 cut(s) 428, 1479
Eco81I CCTNAGG 2 cut(s) 1777, 1915
Eco88I CYCGRG 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 1673
EcoT14I CCWWGG 9 cut(s) 326, 520, 854, 1434, 1509, 1602, 1959, 2076, 2100
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 4 cut(s) 840, 1815, 1863, 2239
EgeI GGCGCC 1 cut(s) 1251
EheI GGCGCC 1 cut(s) 1251
ErhI CCWWGG 9 cut(s) 326, 520, 854, 1434, 1509, 1602, 1959, 2076, 2100
Esp3I CGTCTC 1 cut(s) 1674
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 5 cut(s) 404, 584, 626, 1360, 1659
FauI CCCGC 3 cut(s) 626, 1093, 1759
FbaI TGATCA 1 cut(s) 2142
FblI GTMKAC 2 cut(s) 862, 2256
FriOI GRGCYC 4 cut(s) 840, 1815, 1863, 2239
FspBI CTAG 4 cut(s) 95, 132, 327, 2260
GlaI GCGC 6 cut(s) 238, 1017, 1078, 1251, 1563, 2187
GsaI CCCAGC 1 cut(s) 13
GsuI CTGGAG 1 cut(s) 1066
HaeII RGCGCY 2 cut(s) 1253, 1565
HgaI GACGC 6 cut(s) 117, 266, 607, 748, 1116, 1691
HhaI GCGC 6 cut(s) 239, 1018, 1079, 1252, 1564, 2188
Hin1I GRCGYC 2 cut(s) 1127, 1250
Hin6I GCGC 6 cut(s) 237, 1016, 1077, 1250, 1562, 2186
HinP1I GCGC 6 cut(s) 237, 1016, 1077, 1250, 1562, 2186
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
Hpy166II GTNNAC 8 cut(s) 112, 466, 597, 652, 783, 863, 886, 2257
Hpy8I GTNNAC 8 cut(s) 112, 466, 597, 652, 783, 863, 886, 2257
Hpy99I CGWCG 8 cut(s) 311, 314, 712, 1132, 1414, 2035, 2038, 2044
HpyAV CCTTC 6 cut(s) 827, 1158, 1664, 1688, 1873, 2210
HpyCH4III ACNGT 8 cut(s) 372, 449, 542, 773, 811, 1174, 1703, 1838
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 8 cut(s) 53, 486, 802, 844, 917, 971, 1106, 1364
HpyF3I CTNAG 5 cut(s) 301, 1136, 1206, 1777, 1915
HpySE526I ACGT 1 cut(s) 501
Hsp92I GRCGYC 2 cut(s) 1127, 1250
HspAI GCGC 6 cut(s) 237, 1016, 1077, 1250, 1562, 2186
KasI GGCGCC 1 cut(s) 1249
KpnI GGTACC 1 cut(s) 1383
Ksp22I TGATCA 1 cut(s) 2142
KspI CCGCGG 1 cut(s) 1245
Kzo9I GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
LmnI GCTCC 4 cut(s) 77, 1289, 1414, 1810
MaeI CTAG 4 cut(s) 95, 132, 327, 2260
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 7 cut(s) 502, 805, 997, 1174, 1514, 1832, 2090
MalI GATC 9 cut(s) 93, 377, 401, 527, 706, 1051, 1500, 1593, 2144
MboI GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 3 cut(s) 1049, 1498, 1591
MhlI GDGCHC 5 cut(s) 771, 840, 1815, 1863, 2239
MlsI TGGCCA 1 cut(s) 2168
MluCI AATT 3 cut(s) 261, 670, 1036
MluNI TGGCCA 1 cut(s) 2168
Mly113I GGCGCC 1 cut(s) 1250
MmeI TCCRAC 5 cut(s) 321, 367, 1392, 1644, 1803
Mox20I TGGCCA 1 cut(s) 2168
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 3 cut(s) 431, 731, 1147
MscI TGGCCA 1 cut(s) 2168
MseI TTAA 3 cut(s) 819, 947, 1199
MslI CAYNNNNRTG 1 cut(s) 1011
Msp20I TGGCCA 1 cut(s) 2168
MspA1I CMGCKG 5 cut(s) 142, 406, 1244, 1630, 1642
MspCI CTTAAG 1 cut(s) 946
MspR9I CCNGG 4 cut(s) 333, 553, 589, 1677
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 855
MvnI CGCG 2 cut(s) 1244, 2188
NarI GGCGCC 1 cut(s) 1250
NciI CCSGG 4 cut(s) 333, 553, 589, 1677
NcoI CCATGG 2 cut(s) 520, 2100
NdeII GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
NlaIV GGNNCC 7 cut(s) 337, 1251, 1291, 1381, 1675, 1812, 1840
NmuCI GTSAC 7 cut(s) 502, 805, 997, 1174, 1514, 1832, 2090
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 725
PaeI GCATGC 1 cut(s) 802
PagI TCATGA 4 cut(s) 423, 676, 735, 745
PceI AGGCCT 1 cut(s) 2075
PcsI WCGNNNNNNNCGW 1 cut(s) 716
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 3 cut(s) 431, 731, 1147
PfeI GAWTC 5 cut(s) 776, 1316, 1484, 1994, 2064
PflFI GACNNNGTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 1675
PinAI ACCGGT 2 cut(s) 1324, 1382
PluTI GGCGCC 1 cut(s) 1253
PpuMI RGGWCCY 1 cut(s) 1673
Psp5II RGGWCCY 1 cut(s) 1673
PspFI CCCAGC 1 cut(s) 9
PspN4I GGNNCC 7 cut(s) 337, 1251, 1291, 1381, 1675, 1812, 1840
PspPI GGNCC 4 cut(s) 109, 330, 479, 1673
PspPPI RGGWCCY 1 cut(s) 1673
PstNI CAGNNNCTG 1 cut(s) 226
PsuI RGATCY 3 cut(s) 1049, 1498, 1591
PsyI GACNNNGTC 1 cut(s) 601
PvuII CAGCTG 2 cut(s) 142, 406
RsaI GTAC 2 cut(s) 102, 1381
RsaNI GTAC 2 cut(s) 101, 1380
RseI CAYNNNNRTG 1 cut(s) 1011
SacII CCGCGG 1 cut(s) 1245
SaqAI TTAA 3 cut(s) 819, 947, 1199
Sau3AI GATC 9 cut(s) 91, 375, 399, 525, 704, 1049, 1498, 1591, 2142
Sau96I GGNCC 4 cut(s) 109, 330, 479, 1673
ScrFI CCNGG 4 cut(s) 333, 553, 589, 1677
SduI GDGCHC 5 cut(s) 771, 840, 1815, 1863, 2239
SfoI GGCGCC 1 cut(s) 1251
Sfr303I CCGCGG 1 cut(s) 1245
SfuI TTCGAA 1 cut(s) 725
SgrBI CCGCGG 1 cut(s) 1245
SinI GGWCC 3 cut(s) 109, 479, 1673
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 2 cut(s) 946, 1730
SmoI CTYRAG 2 cut(s) 946, 1730
SphI GCATGC 1 cut(s) 802
Sse9I AATT 3 cut(s) 261, 670, 1036
SseBI AGGCCT 1 cut(s) 2075
SspDI GGCGCC 1 cut(s) 1249
SspMI CTAG 4 cut(s) 95, 132, 327, 2260
StuI AGGCCT 1 cut(s) 2075
StyD4I CCNGG 4 cut(s) 331, 551, 587, 1675
StyI CCWWGG 9 cut(s) 326, 520, 854, 1434, 1509, 1602, 1959, 2076, 2100
TaaI ACNGT 8 cut(s) 372, 449, 542, 773, 811, 1174, 1703, 1838
TaiI ACGT 1 cut(s) 504
TaqI TCGA 7 cut(s) 623, 690, 707, 725, 987, 1130, 2239
TasI AATT 3 cut(s) 261, 670, 1036
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 5 cut(s) 776, 1316, 1484, 1994, 2064
Tru1I TTAA 3 cut(s) 819, 947, 1199
Tru9I TTAA 3 cut(s) 819, 947, 1199
TscAI CASTG 6 cut(s) 375, 778, 814, 1144, 1183, 1302
TseFI GTSAC 7 cut(s) 502, 805, 997, 1174, 1514, 1832, 2090
Tsp45I GTSAC 7 cut(s) 502, 805, 997, 1174, 1514, 1832, 2090
TspGWI ACGGA 3 cut(s) 573, 800, 2038
TspRI CASTG 6 cut(s) 375, 778, 814, 1144, 1183, 1302
Tth111I GACNNNGTC 1 cut(s) 601
Van91I CCANNNNNTGG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 946
VpaK11BI GGWCC 3 cut(s) 109, 479, 1673
XceI RCATGY 1 cut(s) 802
XmaJI CCTAGG 1 cut(s) 326
XmiI GTMKAC 2 cut(s) 862, 2256
XmnI GAANNNNTTC 3 cut(s) 431, 731, 1147
XspI CTAG 4 cut(s) 95, 132, 327, 2260
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.