pycom111g01650

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015791
Physical Location & Seq
Forward (+)
1020259 .. 1022077
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01650.1

Sequence Viewer

Length: 1215 bp
ATGGCTCAGCTCTCACTTTTGCTTACAATTTTTCTCATAACTTCTTTAAGTGCAACTTTTGTTTCCTCTTCACAAGTTCAAGACCCTGAACGAGTAGTAGAGGAGGTCCATAGGAGCATCAATGCCTCCAGGAGGAACTTGGCCTACTTGTCTTGTGGAACTGGCAACCCAATTGATGATTGCTGGAGGTGTGATCCCAACTGGGAAAGAAACCGCCAGCGCTTTGCCGACTGTGCCATTGGCTTCGGCAAGGACGCCATCGGAGGAAGAAACGGTCGAATCTACGTCGTCACCGATTCCGGCGACGACGACCCAGTAAACCCTAAGCCAGGGACCCTAAGACATGCAGTGATCCAGGACGAGCCGTTGTGGATCATATTCAAAAGGGACATGGTGGTTCAGTTAAAACAAGAGCTAGTTATGAACTCGTTCAAGACCATCGACGGCAGAGGTGCGAGCGTCCACATTGCTGGAGGGCCATGCATCACCATACATTACGCTACCAACATCATCATCCATGGCATCCACATACACGACTGCAAACCGGCGGGGAATGGGAACATAAGAAACTCACCGGAGCACTCGGGGTGGTGGACGGTGTCGGACGGTGACGGTGTATCCATTTTTAATGGGCAGCATATATGGGTGGACCATTGCTCGCTGTCGAATTGCCATGATGGACTCATTGATGCCATACATGGATCCACGGCCATCACCATATCGAATAACTACATGACTCATCATGATAAGGTCATGCTTTTGGGACATAGTGATTCGTATACACAAGACAAGAACATGCAAGTCACTATTGCCTTTAATCATTTTGGAGAAGGCCTTGTCCAAAGAATGCCAAGGTGTAGACATGGATATTTTCATGTGGTAAACAATGACTACACACACTGGGAAATGTACGCAATAGGAGGGAGTGCTTCCCCAACTATCCTCAGCCAAGGCAATAGGTTTCTTGCACCGGATAGACGTTTTGATAAAGAGGTGACTAAACATGAGGATGCACCAGAAAGTGAGTGGAGGAAGTGGAACTGGAGGTCAGAGGGGGATATGCTGTTGAACGGGGCTTTTTTCAGGCAATCAGGAGCAGGAGCCTCTTCAAGCTATGCCAGGGCTTCCAGCCTCAGTGCAAGGCCTTCTTCTCTTGTGGGTTCCATCACCACCGCCGCCGGTGCCCTTAACTGCAGGAGGGGATCCCGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

405

Amino Acids

44.79

Weight (kDa)

6.84

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 137 - 325 2.7e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27400 AT3G27400
fragaria_vesca FvH4_6g52190
malus_domestica MD09G1019900.v1.1
prunus_persica Prupe.3G296600_v2.0.a1
pyrus_communis pycom111g01650
rosa_chinensis RchiOBHm_Chr2g0173681
rosa_laevigata RLG00000022214
rosa_multiflora Rmu_sc0002538.1_g000007
rosa_rugosa Rorug02G0572300
rosa_samantha Rh2AG651200 Rh2BG661600 Rh2CG626600 Rh2DG676900
rosa_wichuraiana Rw2G053440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1181
AccI GTMKAC 2 cut(s) 779, 859
AciI CCGC 5 cut(s) 214, 548, 1173, 1176, 1207
AclWI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
AcoI YGGCCR 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 911
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 132, 329
AgsI TTSAA 5 cut(s) 80, 382, 433, 1069, 1110
AjnI CCWGG 4 cut(s) 128, 328, 354, 1118
AluBI AGCT 3 cut(s) 10, 415, 1113
AluI AGCT 3 cut(s) 10, 415, 1113
Alw21I GWGCWC 1 cut(s) 582
AlwI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
Ama87I CYCGRG 1 cut(s) 583
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 5 cut(s) 141, 476, 708, 832, 1142
ApeKI GCWGC 2 cut(s) 634, 1209
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 4 cut(s) 106, 333, 476, 649
AsuHPI GGTGA 7 cut(s) 283, 478, 564, 620, 706, 1006, 1159
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 3 cut(s) 106, 333, 649
BaeGI GKGCMC 1 cut(s) 1186
BamHI GGATCC 2 cut(s) 701, 1202
BanI GGYRCC 1 cut(s) 1181
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 2 cut(s) 646, 1196
BccI CCATC 5 cut(s) 266, 446, 671, 719, 1172
BceAI ACGGC 3 cut(s) 349, 460, 723
BciT130I CCWGG 4 cut(s) 130, 330, 356, 1120
BciVI GTATCC 1 cut(s) 628
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 1192
BfoI RGCGCY 1 cut(s) 223
BfuI GTATCC 1 cut(s) 628
BisI GCNGC 3 cut(s) 635, 1176, 1210
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 3 cut(s) 636, 1177, 1211
Bme1390I CCNGG 4 cut(s) 130, 330, 356, 1120
Bme18I GGWCC 3 cut(s) 106, 333, 649
BmeT110I CYCGRG 1 cut(s) 583
BmgT120I GGNCC 4 cut(s) 106, 333, 476, 649
BmiI GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
BmrFI CCNGG 4 cut(s) 130, 330, 356, 1120
BmrI ACTGGG 3 cut(s) 211, 308, 910
BmsI GCATC 5 cut(s) 126, 492, 531, 679, 1000
BmuI ACTGGG 3 cut(s) 211, 308, 910
BpmI CTGGAG 4 cut(s) 112, 205, 492, 1063
Bpu10I CCTNAGC 2 cut(s) 324, 944
Bpu1102I GCTNAGC 1 cut(s) 6
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
BsaWI WCCGGW 2 cut(s) 574, 970
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 329
Bse118I RCCGGY 2 cut(s) 544, 1178
Bse1I ACTGG 5 cut(s) 166, 206, 314, 905, 1046
Bse3DI GCAATG 2 cut(s) 465, 652
BseBI CCWGG 4 cut(s) 130, 330, 356, 1120
BseDI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
BseGI GGATG 3 cut(s) 513, 522, 1015
BseLI CCNNNNNNNGG 2 cut(s) 132, 329
BseMI GCAATG 2 cut(s) 465, 652
BseMII CTCAG 3 cut(s) 20, 958, 1147
BseNI ACTGG 5 cut(s) 166, 206, 314, 905, 1046
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 1186
BseXI GCAGC 2 cut(s) 646, 1196
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BshNI GGYRCC 1 cut(s) 1181
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 5 cut(s) 300, 545, 575, 971, 1179
BslFI GGGAC 3 cut(s) 346, 401, 777
BslI CCNNNNNNNGG 2 cut(s) 132, 329
BsmFI GGGAC 3 cut(s) 346, 401, 777
BsmI GAATGC 1 cut(s) 852
BsnI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 2 cut(s) 582, 1186
Bsp143I GATC 5 cut(s) 193, 351, 372, 701, 1202
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 5 cut(s) 214, 548, 1173, 1176, 1207
BspANI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BspCNI CTCAG 3 cut(s) 19, 957, 1146
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
BspMAI CTGCAG 1 cut(s) 1196
BspPI GGATC 7 cut(s) 188, 346, 380, 696, 709, 1197, 1210
BspT107I GGYRCC 1 cut(s) 1181
BsrDI GCAATG 2 cut(s) 465, 652
BsrFI RCCGGY 2 cut(s) 544, 1178
BsrI ACTGG 5 cut(s) 166, 206, 314, 905, 1046
BssAI RCCGGY 2 cut(s) 544, 1178
BssECI CCNNGG 6 cut(s) 329, 517, 705, 851, 949, 1119
BssMI GATC 5 cut(s) 193, 351, 372, 701, 1202
BssNAI GTATAC 1 cut(s) 780
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 3 cut(s) 517, 851, 949
Bst1107I GTATAC 1 cut(s) 780
Bst2UI CCWGG 4 cut(s) 130, 330, 356, 1120
Bst4CI ACNGT 5 cut(s) 233, 275, 598, 608, 614
Bst6I CTCTTC 2 cut(s) 73, 1111
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 3 cut(s) 218, 457, 659
BstDEI CTNAG 5 cut(s) 6, 324, 338, 944, 1133
BstDSI CCRYGG 2 cut(s) 517, 705
BstENI CCTNNNNNAGG 2 cut(s) 130, 327
BstF5I GGATG 3 cut(s) 513, 522, 1015
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 5 cut(s) 196, 354, 375, 704, 1205
BstMBI GATC 5 cut(s) 193, 351, 372, 701, 1202
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 233, 1181
BstNI CCWGG 4 cut(s) 130, 330, 356, 1120
BstNSI RCATGY 2 cut(s) 347, 799
BstSCI CCNGG 4 cut(s) 128, 328, 354, 1118
BstSFI CTRYAG 1 cut(s) 1192
BstSLI GKGCMC 1 cut(s) 1186
BstV1I GCAGC 2 cut(s) 646, 1196
BstX2I RGATCY 2 cut(s) 701, 1202
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 2 cut(s) 701, 1202
BstZ17I GTATAC 1 cut(s) 780
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 5 cut(s) 143, 478, 710, 834, 1144
BtgI CCRYGG 2 cut(s) 517, 705
BtsCI GGATG 3 cut(s) 513, 522, 1015
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 354, 898, 1141
Cac8I GCNNGC 3 cut(s) 218, 457, 659
CciI TCATGA 1 cut(s) 742
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 2 cut(s) 544, 1178
Cfr13I GGNCC 4 cut(s) 106, 333, 476, 649
CseI GACGC 2 cut(s) 263, 448
Csp6I GTAC 1 cut(s) 910
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 5 cut(s) 6, 324, 338, 944, 1133
DpnI GATC 5 cut(s) 195, 353, 374, 703, 1204
DpnII GATC 5 cut(s) 193, 351, 372, 701, 1202
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 2 cut(s) 73, 1111
EarI CTCTTC 2 cut(s) 73, 1111
Eco130I CCWWGG 3 cut(s) 517, 851, 949
Eco147I AGGCCT 2 cut(s) 834, 1144
Eco47I GGWCC 3 cut(s) 106, 333, 649
Eco47III AGCGCT 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 583
EcoNI CCTNNNNNAGG 2 cut(s) 130, 327
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 4 cut(s) 128, 328, 354, 1118
EcoT14I CCWWGG 3 cut(s) 517, 851, 949
EcoT22I ATGCAT 1 cut(s) 485
ErhI CCWWGG 3 cut(s) 517, 851, 949
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 1132, 1164
FaqI GGGAC 3 cut(s) 346, 401, 777
FauI CCCGC 1 cut(s) 541
FblI GTMKAC 2 cut(s) 779, 859
Fnu4HI GCNGC 3 cut(s) 635, 1176, 1210
FokI GGATG 3 cut(s) 500, 509, 1022
Fsp4HI GCNGC 3 cut(s) 635, 1176, 1210
FspBI CTAG 1 cut(s) 416
GlaI GCGC 1 cut(s) 221
GluI GCNGC 3 cut(s) 635, 1176, 1210
GsuI CTGGAG 4 cut(s) 112, 205, 492, 1063
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 5 cut(s) 143, 478, 710, 834, 1144
HapII CCGG 5 cut(s) 300, 545, 575, 971, 1179
HgaI GACGC 2 cut(s) 263, 448
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 255
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 5 cut(s) 279, 296, 681, 736, 773
HpaII CCGG 5 cut(s) 300, 545, 575, 971, 1179
HphI GGTGA 7 cut(s) 283, 478, 564, 620, 706, 1006, 1159
Hpy166II GTNNAC 7 cut(s) 319, 463, 594, 649, 780, 860, 883
Hpy188I TCNGA 3 cut(s) 263, 604, 1051
Hpy188III TCNNGA 4 cut(s) 80, 433, 743, 1092
Hpy8I GTNNAC 7 cut(s) 319, 463, 594, 649, 780, 860, 883
Hpy99I CGWCG 4 cut(s) 290, 308, 311, 446
HpyAV CCTTC 2 cut(s) 824, 1155
HpyCH4III ACNGT 5 cut(s) 233, 275, 598, 608, 614
HpyCH4IV ACGT 2 cut(s) 285, 979
HpyCH4V TGCA 9 cut(s) 53, 347, 483, 540, 799, 968, 1013, 1139, 1194
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 1181
HpyF3I CTNAG 5 cut(s) 6, 324, 338, 944, 1133
HpySE526I ACGT 2 cut(s) 285, 979
Hsp92I GRCGYC 1 cut(s) 255
HspAI GCGC 1 cut(s) 220
KflI GGGWCCC 1 cut(s) 333
Kzo9I GATC 5 cut(s) 193, 351, 372, 701, 1202
LmnI GCTCC 4 cut(s) 114, 577, 1094, 1100
Lsp1109I GCAGC 2 cut(s) 646, 1196
LweI GCATC 5 cut(s) 126, 492, 531, 679, 1000
MaeI CTAG 1 cut(s) 416
MaeII ACGT 2 cut(s) 285, 979
MaeIII GTNAC 4 cut(s) 289, 608, 802, 994
MalI GATC 5 cut(s) 195, 353, 374, 703, 1204
MboI GATC 5 cut(s) 193, 351, 372, 701, 1202
MboII GAAGA 4 cut(s) 60, 279, 1098, 1140
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 2 cut(s) 701, 1202
MhlI GDGCHC 2 cut(s) 582, 1186
MluCI AATT 3 cut(s) 27, 171, 667
MlyI GAGTC 2 cut(s) 675, 730
MmeI TCCRAC 1 cut(s) 582
Mph1103I ATGCAT 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 5 cut(s) 47, 404, 627, 816, 1188
MslI CAYNNNNRTG 1 cut(s) 1008
MspA1I CMGCKG 1 cut(s) 1209
MspI CCGG 5 cut(s) 300, 545, 575, 971, 1179
MspR9I CCNGG 4 cut(s) 130, 330, 356, 1120
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 852
MvaI CCWGG 4 cut(s) 130, 330, 356, 1120
MwoI GCNNNNNNNGC 2 cut(s) 233, 1181
NcoI CCATGG 1 cut(s) 517
NdeII GATC 5 cut(s) 193, 351, 372, 701, 1202
NlaIV GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
NmuCI GTSAC 4 cut(s) 289, 608, 802, 994
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 2 cut(s) 347, 799
PagI TCATGA 1 cut(s) 742
PceI AGGCCT 2 cut(s) 834, 1144
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 852
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 3 cut(s) 279, 296, 773
PflFI GACNNNGTC 1 cut(s) 598
PfoI TCCNGGA 2 cut(s) 128, 354
PkrI GCNGC 3 cut(s) 636, 1177, 1211
PleI GAGTC 2 cut(s) 675, 730
PpsI GAGTC 2 cut(s) 675, 730
PpuMI RGGWCCY 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 333
Psp6I CCWGG 4 cut(s) 128, 328, 354, 1118
PspGI CCWGG 4 cut(s) 128, 328, 354, 1118
PspN4I GGNNCC 7 cut(s) 334, 335, 703, 1102, 1162, 1183, 1204
PspPI GGNCC 4 cut(s) 106, 333, 476, 649
PspPPI RGGWCCY 1 cut(s) 333
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PstI CTGCAG 1 cut(s) 1196
PsuI RGATCY 2 cut(s) 701, 1202
PsyI GACNNNGTC 1 cut(s) 598
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 1 cut(s) 1008
SaqAI TTAA 5 cut(s) 47, 404, 627, 816, 1188
SatI GCNGC 3 cut(s) 635, 1176, 1210
Sau3AI GATC 5 cut(s) 193, 351, 372, 701, 1202
Sau96I GGNCC 4 cut(s) 106, 333, 476, 649
SchI GAGTC 2 cut(s) 675, 730
ScrFI CCNGG 4 cut(s) 130, 330, 356, 1120
SduI GDGCHC 2 cut(s) 582, 1186
SfaNI GCATC 5 cut(s) 126, 492, 531, 679, 1000
SfcI CTRYAG 1 cut(s) 1192
SgrAI CRCCGGYG 1 cut(s) 1178
SinI GGWCC 3 cut(s) 106, 333, 649
SmiMI CAYNNNNRTG 1 cut(s) 1008
Sse9I AATT 3 cut(s) 27, 171, 667
SseBI AGGCCT 2 cut(s) 834, 1144
SsiI CCGC 5 cut(s) 214, 548, 1173, 1176, 1207
SspMI CTAG 1 cut(s) 416
StuI AGGCCT 2 cut(s) 834, 1144
StyD4I CCNGG 4 cut(s) 128, 328, 354, 1118
StyI CCWWGG 3 cut(s) 517, 851, 949
TaaI ACNGT 5 cut(s) 233, 275, 598, 608, 614
TaiI ACGT 2 cut(s) 288, 982
TaqI TCGA 4 cut(s) 277, 441, 665, 722
TasI AATT 3 cut(s) 27, 171, 667
TauI GCSGC 1 cut(s) 1178
TfiI GAWTC 3 cut(s) 279, 296, 773
Tru1I TTAA 5 cut(s) 47, 404, 627, 816, 1188
Tru9I TTAA 5 cut(s) 47, 404, 627, 816, 1188
TscAI CASTG 3 cut(s) 354, 905, 1141
TseFI GTSAC 4 cut(s) 289, 608, 802, 994
TseI GCWGC 2 cut(s) 634, 1209
Tsp45I GTSAC 4 cut(s) 289, 608, 802, 994
TspDTI ATGAA 2 cut(s) 437, 863
TspRI CASTG 3 cut(s) 354, 905, 1141
Tth111I GACNNNGTC 1 cut(s) 598
VpaK11BI GGWCC 3 cut(s) 106, 333, 649
XagI CCTNNNNNAGG 2 cut(s) 130, 327
XceI RCATGY 2 cut(s) 347, 799
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 1023
XmiI GTMKAC 2 cut(s) 779, 859
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.