Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005148

Physical Location & Seq

Genomic Coordinates Forward (+)

1982104 .. 1985891

Gene Structure 3788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1032700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGCAACTTGTATACCCGCGGGAAGTGGCAGACTCCACTGTAGAGTGAGAGACGTCAAGGGACAAGGTTGTAGAAGGACTTGTTGGATTGCCAGTGTTGCATTTAGTGGATGCATACAGAAAGACTTTTCATGGTCACACGGGGTATCAAAGTTCTTGCAGATCCAAAGATGTAACTTTTCCCCATCTCCAATTAACGAGAACTCAAAGACCTTAAGAACAGTCACTTCATCGAGCTTGAGAGATGACTCCACCAAGTTTTTTGATTTTGCTGTCATTGGTAGTGGAGTTGCCGGGCTCCGCTATGCCCTTGAAGTGGCAAAACATGGATCTGTAGCCGTGATTACCAAGGCTGAACCTCATGAGAGCAATACAAACTATGCTCAAGGTGGTGTTAGTGCTGTTCTTTCCCTATCAGATTCTGTGGAGAGTCATGTCCAGGACACCATTGTAGCAGGGGCCTATCTCTGTGACGAGGAGACTGTCAGAGTTGTGTGTACAGAAGGACCTGAAAGAATTAGAGAATTAATTGCAATGGGGGCATCCTTTGATCACGGGGAGGATGGAAACTTGCACCTAGCAAGAGAGGGAGGTCACTCTCATCACAGGATTGTTCATGCTGCTGATATGACTGGAAGGGAGATTGAAAGGGCACTGTTGGAGGCCGTTCTTAAGGATCCTAAGATCTTCATGTTTGAACACCATCTTGCGATAGATTTGCTAACTTGTCAGGATGGTTCTGATACAGTTTGTCTTGGCGTTGACACTTTAAATACGGAAACACAAGAGGTGATACGGTTTATCTCAAAGGTGACTTTACTTGCGTCAGGTGGGGCTGGACAAATCTATCCAACAACTACAAATCCTCTGGTAGCCACAGGGGATGGAATTGCTATGGCCCATCGAGCTCAAGCTGTGATTTCCAACATGGAATTTGTTCAGTTCCATCCTACGGCCTTAGCTGACGAGGGCCTTCCTGTCCAACCTACCAAGGCTCGAGAAAATGCATTTCTCATTACTGAAGCTGTCAGGGGTGATGGAGGCATCCTGTACAATTTAGACATGGAAAGGTTCATGCCACTCTATGATGAACGAGCAGAACTTGCTCCGAGGGATGTGGTGGCAAGATGCATAGATGACCAGCTTAAAAAGCGAAATGAGAAGTACGTGCTACTTGATATAAGTCACAAGCCCAGAGAAAAAATCCTCTCACACTTCCCCAACATAGCAGCCGAGTGCCTTAAGTACGGCTTGGACATAACTCGCCAGCCAATTCCCGTTGTTCCTGCTGCTCACTATATGTGTGGGGGAGTCCGTGCTGGACTCCAGGGAGAGACAAATGTGCAGGGCCTGTTCGTGGCAGGTGAAGTTGCTTGCACGGGATTGCATGGAGCCAACCGTCTTGCTAGTAACTCATTGCTTGAAGCACTAGTTTTCGCACGAAGAGCGGTCCACCCCTCAATTGAACACATGAAGTGCTCTAGCCTCGACTTCAGCACTTCAGATTGGTGGGCCAGACCAGCCGTGCCTGTCTCACTCGAGAGCAATGCCATCGAAAAAATTTTGGCAATGACAAGGGAAGTAAGAAAAGAAGTGCAATCGATAATGTGGAAGTATGTTGGAATTGTACGTTCTACAACAAGGCTTGAAACAGCAAAGCAAAAGATTTGTGTTCTGGAGGATAAGTGGGAGGACTACTTATTTCAGATGGGATGGGAACCGACAATGGTGGGGCTCGAGGCTTGCGAAATGAGAAACTTCTTCTGTTGTGCAAAACTGGTGGTGAGCAGCGCCCTTGCTAGGCATGAGAGCCGCGGACTTCACTATACCATCGATTTTCCTGATCTTGAGGAGAGCAAGAGGCTTCCAACAGTTATACTCCCTTCTTCATCCGTGCGAACTTCATGGAGTTCGCGTCAACTACACAAGCTGCCATTGTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009435 GO:0009507 GO:0009536 GO:0009987 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019637 GO:0019674 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046496 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072524 GO:0072525 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00760 ko01100 map00250 map00760 map01100

Pfam Domains

Protein Families

FAD_binding_2 Succ_DH_flav_C

Protein Analysis

648

Amino Acids

71.35

Weight (kDa)

6.33

Isoelectric Point (pI)

38.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_2	PF00890	89 - 473	1.4e-89	FAD binding domain
DAO	PF01266	89 - 197	6.7e-08	FAD dependent oxidoreductase
Succ_DH_flav_C	PF02910	529 - 618	4.4e-16	Fumarate reductase flavoprotein C-term

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14760
fragaria_vesca	FvH4_6g50750 FvH4_6g50750 FvH4_6g50750 FvH4_6g50750 FvH4_6g50750
malus_domestica	MD09G1032700.v1.1 MD17G1034300.v1.1
prunus_persica	Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1
pyrus_communis	pycom111g02610 pycom17g03170
rosa_chinensis	RchiOBHm_Chr2g0171691
rosa_laevigata	RLG00000022029
rosa_multiflora	Rmu_ssc0000252.1_g000010
rosa_roxburghii	Rroxscaffold_2G00080520
rosa_rugosa	Rorug02G0555400
rosa_samantha	Rh2AG630400 Rh2BG640900 Rh2BG643400 Rh2CG609100 Rh2DG651800
rosa_wichuraiana	Rw2G052170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1352
AatII	GACGTC	1 cut(s)	57
Acc36I	ACCTGC	1 cut(s)	1352
AccBSI	CCGCTC	1 cut(s)	1448
AccI	GTMKAC	1 cut(s)	13
AccII	CGCG	3 cut(s)	20, 1815, 1915
AciI	CCGC	6 cut(s)	18, 20, 301, 1448, 1813, 1815
AclWI	GGATC	4 cut(s)	157, 337, 671, 684
AcsI	RAATTY	2 cut(s)	932, 1560
AcuI	CTGAAG	3 cut(s)	1041, 1477, 1485
AcyI	GRCGYC	1 cut(s)	54
AfaI	GTAC	5 cut(s)	499, 1052, 1166, 1247, 1629
AfiI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
AflII	CTTAAG	3 cut(s)	214, 671, 1241
AgsI	TTSAA	6 cut(s)	314, 647, 698, 1424, 1466, 1649
AhlI	ACTAGT	1 cut(s)	1429
AjnI	CCWGG	2 cut(s)	438, 1326
AjuI	GAANNNNNNNTTGG	2 cut(s)	67, 99
AluBI	AGCT	7 cut(s)	237, 908, 914, 962, 1025, 1144, 1930
AluI	AGCT	7 cut(s)	237, 908, 914, 962, 1025, 1144, 1930
Alw21I	GWGCWC	2 cut(s)	910, 1481
Alw26I	GTCTC	4 cut(s)	45, 473, 1328, 1537
AlwI	GGATC	4 cut(s)	157, 337, 671, 684
AlwNI	CAGNNNCTG	2 cut(s)	422, 1351
Ama87I	CYCGRG	3 cut(s)	996, 1538, 1736
AoxI	GGCC	7 cut(s)	459, 663, 897, 954, 970, 1348, 1512
ApeKI	GCWGC	5 cut(s)	620, 1229, 1289, 1788, 1930
ApoI	RAATTY	2 cut(s)	932, 1560
AseI	ATTAAT	1 cut(s)	527
Asp700I	GAANNNNTTC	1 cut(s)	936
AspLEI	GCGC	1 cut(s)	1793
AspS9I	GGNCC	7 cut(s)	459, 506, 898, 970, 1348, 1450, 1512
AsuC2I	CCSGG	1 cut(s)	295
AsuHPI	GGTGA	5 cut(s)	802, 823, 1046, 1376, 1795
AvaI	CYCGRG	3 cut(s)	996, 1538, 1736
AvaII	GGWCC	2 cut(s)	506, 1450
BaeGI	GKGCMC	1 cut(s)	655
BamHI	GGATCC	1 cut(s)	676
BanII	GRGCYC	3 cut(s)	300, 910, 1737
Bbv12I	GWGCWC	2 cut(s)	910, 1481
BbvI	GCAGC	5 cut(s)	607, 1241, 1276, 1800, 1917
BceAI	ACGGC	5 cut(s)	323, 650, 969, 1264, 1508
BcgI	CGANNNNNNTGC	6 cut(s)	700, 734, 1529, 1534, 1563, 1568
BciT130I	CCWGG	2 cut(s)	440, 1328
BclI	TGATCA	1 cut(s)	550
BcnI	CCSGG	1 cut(s)	295
BcoDI	GTCTC	4 cut(s)	45, 473, 1328, 1537
BcuI	ACTAGT	1 cut(s)	1429
BfaI	CTAG	5 cut(s)	578, 1407, 1430, 1482, 1800
BfmI	CTRYAG	2 cut(s)	40, 333
BfoI	RGCGCY	1 cut(s)	1794
BfrI	CTTAAG	3 cut(s)	214, 671, 1241
BfuAI	ACCTGC	1 cut(s)	1352
BglII	AGATCT	1 cut(s)	684
BisI	GCNGC	6 cut(s)	621, 1230, 1290, 1789, 1813, 1931
BlsI	GCNGC	6 cut(s)	622, 1231, 1291, 1790, 1814, 1932
Bme1390I	CCNGG	3 cut(s)	295, 440, 1328
Bme18I	GGWCC	2 cut(s)	506, 1450
BmeT110I	CYCGRG	3 cut(s)	996, 1538, 1736
BmgT120I	GGNCC	7 cut(s)	459, 506, 898, 970, 1348, 1450, 1512
BmiI	GGNNCC	5 cut(s)	299, 460, 678, 1393, 1719
BmrFI	CCNGG	3 cut(s)	295, 440, 1328
BmsI	GCATC	4 cut(s)	101, 551, 1053, 1118
BpmI	CTGGAG	2 cut(s)	1310, 1697
Bpu10I	CCTNAGC	1 cut(s)	958
BpuEI	CTTGAG	4 cut(s)	259, 369, 894, 1868
BpuMI	CCSGG	1 cut(s)	295
Bsa29I	ATCGAT	2 cut(s)	1601, 1833
BsaAI	YACGTR	1 cut(s)	1168
BsaHI	GRCGYC	1 cut(s)	54
BsaJI	CCNNGG	6 cut(s)	18, 348, 990, 1109, 1327, 1813
BsaXI	ACNNNNNCTCC	4 cut(s)	1670, 1700, 1900, 1930
Bsc4I	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
Bse1I	ACTGG	3 cut(s)	93, 637, 1782
Bse3DI	GCAATG	4 cut(s)	540, 1415, 1552, 1575
BseBI	CCWGG	2 cut(s)	440, 1328
BseCI	ATCGAT	2 cut(s)	1601, 1833
BseDI	CCNNGG	6 cut(s)	18, 348, 990, 1109, 1327, 1813
BseLI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
BseMI	GCAATG	4 cut(s)	540, 1415, 1552, 1575
BseNI	ACTGG	3 cut(s)	93, 637, 1782
BseRI	GAGGAG	2 cut(s)	491, 1865
BseSI	GKGCMC	1 cut(s)	655
BseXI	GCAGC	5 cut(s)	607, 1241, 1276, 1800, 1917
BsgI	GTGCAG	1 cut(s)	1364
Bsh1236I	CGCG	3 cut(s)	20, 1815, 1915
BshFI	GGCC	7 cut(s)	461, 665, 899, 956, 972, 1350, 1514
BshVI	ATCGAT	2 cut(s)	1601, 1833
BsiHKAI	GWGCWC	2 cut(s)	910, 1481
BsiHKCI	CYCGRG	3 cut(s)	996, 1538, 1736
BsiSI	CCGG	1 cut(s)	294
BslFI	GGGAC	1 cut(s)	75
BslI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
BsmAI	GTCTC	4 cut(s)	45, 473, 1328, 1537
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	75
BsnI	GGCC	7 cut(s)	461, 665, 899, 956, 972, 1350, 1514
BsoBI	CYCGRG	3 cut(s)	996, 1538, 1736
Bsp1286I	GDGCHC	5 cut(s)	300, 655, 910, 1481, 1737
Bsp1407I	TGTACA	2 cut(s)	497, 1050
Bsp143I	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
BspACI	CCGC	6 cut(s)	18, 20, 301, 1448, 1813, 1815
BspANI	GGCC	7 cut(s)	461, 665, 899, 956, 972, 1350, 1514
BspDI	ATCGAT	2 cut(s)	1601, 1833
BspFNI	CGCG	3 cut(s)	20, 1815, 1915
BspHI	TCATGA	1 cut(s)	361
BspLI	GGNNCC	5 cut(s)	299, 460, 678, 1393, 1719
BspMI	ACCTGC	1 cut(s)	1352
BspPI	GGATC	4 cut(s)	157, 337, 671, 684
BspQI	GCTCTTC	1 cut(s)	1438
BspTI	CTTAAG	3 cut(s)	214, 671, 1241
BsrBI	CCGCTC	1 cut(s)	1448
BsrDI	GCAATG	4 cut(s)	540, 1415, 1552, 1575
BsrGI	TGTACA	2 cut(s)	497, 1050
BsrI	ACTGG	3 cut(s)	93, 637, 1782
BssECI	CCNNGG	6 cut(s)	18, 348, 990, 1109, 1327, 1813
BssMI	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
BssNAI	GTATAC	1 cut(s)	14
BssNI	GRCGYC	1 cut(s)	54
BssT1I	CCWWGG	2 cut(s)	348, 990
Bst1107I	GTATAC	1 cut(s)	14
Bst2UI	CCWGG	2 cut(s)	440, 1328
Bst4CI	ACNGT	8 cut(s)	41, 223, 484, 657, 748, 798, 1400, 1873
Bst6I	CTCTTC	1 cut(s)	1438
BstACI	GRCGYC	1 cut(s)	54
BstAFI	CTTAAG	3 cut(s)	214, 671, 1241
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	2 cut(s)	497, 1050
BstBAI	YACGTR	1 cut(s)	1168
BstC8I	GCNNGC	3 cut(s)	1268, 1375, 1744
BstDEI	CTNAG	2 cut(s)	681, 958
BstDSI	CCRYGG	2 cut(s)	18, 1813
BstFNI	CGCG	3 cut(s)	20, 1815, 1915
BstH2I	RGCGCY	1 cut(s)	1794
BstHHI	GCGC	1 cut(s)	1793
BstKTI	GATC	6 cut(s)	165, 332, 553, 679, 687, 1846
BstMAI	GTCTC	4 cut(s)	45, 473, 1328, 1537
BstMBI	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
BstMWI	GCNNNNNNNGC	7 cut(s)	98, 539, 905, 1103, 1150, 1445, 1520
BstNI	CCWGG	2 cut(s)	440, 1328
BstSCI	CCNGG	3 cut(s)	293, 438, 1326
BstSFI	CTRYAG	2 cut(s)	40, 333
BstSLI	GKGCMC	1 cut(s)	655
BstUI	CGCG	3 cut(s)	20, 1815, 1915
BstV1I	GCAGC	5 cut(s)	607, 1241, 1276, 1800, 1917
BstX2I	RGATCY	4 cut(s)	162, 329, 676, 684
BstYI	RGATCY	4 cut(s)	162, 329, 676, 684
BstZ17I	GTATAC	1 cut(s)	14
Bsu15I	ATCGAT	2 cut(s)	1601, 1833
BsuRI	GGCC	7 cut(s)	461, 665, 899, 956, 972, 1350, 1514
BsuTUI	ATCGAT	2 cut(s)	1601, 1833
BtgI	CCRYGG	2 cut(s)	18, 1813
BtsIMutI	CAGTG	3 cut(s)	37, 100, 653
BveI	ACCTGC	1 cut(s)	1352
Cac8I	GCNNGC	3 cut(s)	1268, 1375, 1744
CaiI	CAGNNNCTG	2 cut(s)	422, 1351
CciI	TCATGA	1 cut(s)	361
CfoI	GCGC	1 cut(s)	1793
Cfr13I	GGNCC	7 cut(s)	459, 506, 898, 970, 1348, 1450, 1512
Cfr42I	CCGCGG	2 cut(s)	21, 1816
ClaI	ATCGAT	2 cut(s)	1601, 1833
CseI	GACGC	2 cut(s)	813, 1904
Csp6I	GTAC	5 cut(s)	498, 1051, 1165, 1246, 1628
CspCI	CAANNNNNGTGG	2 cut(s)	1761, 1796
CviQI	GTAC	5 cut(s)	498, 1051, 1165, 1246, 1628
DdeI	CTNAG	2 cut(s)	681, 958
DpnI	GATC	6 cut(s)	164, 331, 552, 678, 686, 1845
DpnII	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
DraI	TTTAAA	1 cut(s)	771
Eam1104I	CTCTTC	1 cut(s)	1438
EarI	CTCTTC	1 cut(s)	1438
Ecl136II	GAGCTC	1 cut(s)	908
Eco130I	CCWWGG	2 cut(s)	348, 990
Eco24I	GRGCYC	3 cut(s)	300, 910, 1737
Eco47I	GGWCC	2 cut(s)	506, 1450
Eco53kI	GAGCTC	1 cut(s)	908
Eco57I	CTGAAG	3 cut(s)	1041, 1477, 1485
Eco88I	CYCGRG	3 cut(s)	996, 1538, 1736
EcoICRI	GAGCTC	1 cut(s)	908
EcoO109I	RGGNCCY	4 cut(s)	459, 506, 970, 1348
EcoRII	CCWGG	2 cut(s)	438, 1326
EcoT14I	CCWWGG	2 cut(s)	348, 990
EcoT22I	ATGCAT	3 cut(s)	116, 1009, 1133
EcoT38I	GRGCYC	3 cut(s)	300, 910, 1737
ErhI	CCWWGG	2 cut(s)	348, 990
Esp3I	CGTCTC	1 cut(s)	45
FalI	AAGNNNNNCTT	2 cut(s)	1235, 1267
FaqI	GGGAC	1 cut(s)	75
FauI	CCCGC	2 cut(s)	13, 25
FbaI	TGATCA	1 cut(s)	550
FblI	GTMKAC	1 cut(s)	13
Fnu4HI	GCNGC	6 cut(s)	621, 1230, 1290, 1789, 1813, 1931
FriOI	GRGCYC	3 cut(s)	300, 910, 1737
Fsp4HI	GCNGC	6 cut(s)	621, 1230, 1290, 1789, 1813, 1931
FspBI	CTAG	5 cut(s)	578, 1407, 1430, 1482, 1800
GlaI	GCGC	1 cut(s)	1792
GluI	GCNGC	6 cut(s)	621, 1230, 1290, 1789, 1813, 1931
GsuI	CTGGAG	2 cut(s)	1310, 1697
HaeII	RGCGCY	1 cut(s)	1794
HaeIII	GGCC	7 cut(s)	461, 665, 899, 956, 972, 1350, 1514
HapII	CCGG	1 cut(s)	294
HgaI	GACGC	2 cut(s)	813, 1904
HhaI	GCGC	1 cut(s)	1793
Hin1I	GRCGYC	1 cut(s)	54
Hin6I	GCGC	1 cut(s)	1791
HinP1I	GCGC	1 cut(s)	1791
HincII	GTYRAC	2 cut(s)	763, 1919
HindII	GTYRAC	2 cut(s)	763, 1919
HinfI	GANTC	6 cut(s)	33, 248, 419, 430, 1311, 1323
HpaII	CCGG	1 cut(s)	294
HphI	GGTGA	5 cut(s)	802, 823, 1046, 1376, 1795
Hpy166II	GTNNAC	5 cut(s)	14, 498, 763, 1453, 1919
Hpy188I	TCNGA	6 cut(s)	418, 488, 742, 1110, 1504, 1707
Hpy188III	TCNNGA	7 cut(s)	362, 731, 998, 1540, 1676, 1841, 1847
Hpy8I	GTNNAC	5 cut(s)	14, 498, 763, 1453, 1919
HpyAV	CCTTC	5 cut(s)	69, 497, 630, 983, 1893
HpyCH4III	ACNGT	8 cut(s)	41, 223, 484, 657, 748, 798, 1400, 1873
HpyCH4IV	ACGT	3 cut(s)	54, 1167, 1630
HpyF10VI	GCNNNNNNNGC	7 cut(s)	98, 539, 905, 1103, 1150, 1445, 1520
HpyF3I	CTNAG	2 cut(s)	681, 958
HpySE526I	ACGT	3 cut(s)	54, 1167, 1630
Hsp92I	GRCGYC	1 cut(s)	54
HspAI	GCGC	1 cut(s)	1791
Ksp22I	TGATCA	1 cut(s)	550
KspI	CCGCGG	2 cut(s)	21, 1816
Kzo9I	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
LguI	GCTCTTC	1 cut(s)	1438
LmnI	GCTCC	3 cut(s)	303, 1111, 1391
Lsp1109I	GCAGC	5 cut(s)	607, 1241, 1276, 1800, 1917
LweI	GCATC	4 cut(s)	101, 551, 1053, 1118
MaeI	CTAG	5 cut(s)	578, 1407, 1430, 1482, 1800
MaeII	ACGT	3 cut(s)	54, 1167, 1630
MaeIII	GTNAC	8 cut(s)	135, 173, 223, 470, 593, 811, 1184, 1409
MalI	GATC	6 cut(s)	164, 331, 552, 678, 686, 1845
MbiI	CCGCTC	1 cut(s)	1448
MboI	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
MboII	GAAGA	4 cut(s)	679, 1455, 1753, 1878
MfeI	CAATTG	1 cut(s)	1461
MflI	RGATCY	4 cut(s)	162, 329, 676, 684
MhlI	GDGCHC	5 cut(s)	300, 655, 910, 1481, 1737
MlyI	GAGTC	5 cut(s)	27, 242, 439, 1317, 1320
MmeI	TCCRAC	7 cut(s)	65, 639, 875, 948, 1006, 1600, 1892
Mph1103I	ATGCAT	3 cut(s)	116, 1009, 1133
MroXI	GAANNNNTTC	1 cut(s)	936
MseI	TTAA	7 cut(s)	195, 215, 527, 672, 770, 1146, 1242
MspA1I	CMGCKG	2 cut(s)	20, 1815
MspCI	CTTAAG	3 cut(s)	214, 671, 1241
MspI	CCGG	1 cut(s)	294
MspR9I	CCNGG	3 cut(s)	295, 440, 1328
MunI	CAATTG	1 cut(s)	1461
MvaI	CCWGG	2 cut(s)	440, 1328
MvnI	CGCG	3 cut(s)	20, 1815, 1915
MwoI	GCNNNNNNNGC	7 cut(s)	98, 539, 905, 1103, 1150, 1445, 1520
NciI	CCSGG	1 cut(s)	295
NdeII	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
NlaIV	GGNNCC	5 cut(s)	299, 460, 678, 1393, 1719
NmeAIII	GCCGAG	1 cut(s)	1258
NmuCI	GTSAC	6 cut(s)	135, 223, 470, 593, 811, 1184
NsiI	ATGCAT	3 cut(s)	116, 1009, 1133
PaeR7I	CTCGAG	3 cut(s)	996, 1538, 1736
PagI	TCATGA	1 cut(s)	361
PaqCI	CACCTGC	1 cut(s)	1352
PciSI	GCTCTTC	1 cut(s)	1438
PcsI	WCGNNNNNNNCGW	1 cut(s)	1743
PdmI	GAANNNNTTC	1 cut(s)	936
PfeI	GAWTC	1 cut(s)	419
PfoI	TCCNGGA	1 cut(s)	438
PkrI	GCNGC	6 cut(s)	622, 1231, 1291, 1790, 1814, 1932
PleI	GAGTC	5 cut(s)	27, 242, 438, 1317, 1319
PpsI	GAGTC	5 cut(s)	27, 242, 438, 1317, 1319
Ppu21I	YACGTR	1 cut(s)	1168
PpuMI	RGGWCCY	1 cut(s)	506
PshBI	ATTAAT	1 cut(s)	527
Psp124BI	GAGCTC	1 cut(s)	910
Psp5II	RGGWCCY	1 cut(s)	506
Psp6I	CCWGG	2 cut(s)	438, 1326
PspGI	CCWGG	2 cut(s)	438, 1326
PspN4I	GGNNCC	5 cut(s)	299, 460, 678, 1393, 1719
PspPI	GGNCC	7 cut(s)	459, 506, 898, 970, 1348, 1450, 1512
PspPPI	RGGWCCY	1 cut(s)	506
PspXI	VCTCGAGB	1 cut(s)	1736
PstNI	CAGNNNCTG	2 cut(s)	422, 1351
PsuI	RGATCY	4 cut(s)	162, 329, 676, 684
RsaI	GTAC	5 cut(s)	499, 1052, 1166, 1247, 1629
RsaNI	GTAC	5 cut(s)	498, 1051, 1165, 1246, 1628
SacI	GAGCTC	1 cut(s)	910
SacII	CCGCGG	2 cut(s)	21, 1816
SapI	GCTCTTC	1 cut(s)	1438
SaqAI	TTAA	7 cut(s)	195, 215, 527, 672, 770, 1146, 1242
SatI	GCNGC	6 cut(s)	621, 1230, 1290, 1789, 1813, 1931
Sau3AI	GATC	6 cut(s)	162, 329, 550, 676, 684, 1843
Sau96I	GGNCC	7 cut(s)	459, 506, 898, 970, 1348, 1450, 1512
SchI	GAGTC	5 cut(s)	27, 242, 439, 1317, 1320
ScrFI	CCNGG	3 cut(s)	295, 440, 1328
SduI	GDGCHC	5 cut(s)	300, 655, 910, 1481, 1737
SfaNI	GCATC	4 cut(s)	101, 551, 1053, 1118
SfcI	CTRYAG	2 cut(s)	40, 333
Sfr274I	CTCGAG	3 cut(s)	996, 1538, 1736
Sfr303I	CCGCGG	2 cut(s)	21, 1816
SgrBI	CCGCGG	2 cut(s)	21, 1816
SinI	GGWCC	2 cut(s)	506, 1450
SlaI	CTCGAG	3 cut(s)	996, 1538, 1736
SpeI	ACTAGT	1 cut(s)	1429
SsiI	CCGC	6 cut(s)	18, 20, 301, 1448, 1813, 1815
SspMI	CTAG	5 cut(s)	578, 1407, 1430, 1482, 1800
SstI	GAGCTC	1 cut(s)	910
StyD4I	CCNGG	3 cut(s)	293, 438, 1326
StyI	CCWWGG	2 cut(s)	348, 990
TaaI	ACNGT	8 cut(s)	41, 223, 484, 657, 748, 798, 1400, 1873
TaiI	ACGT	3 cut(s)	57, 1170, 1633
TaqI	TCGA	9 cut(s)	233, 904, 997, 1488, 1539, 1554, 1601, 1737, 1833
TatI	WGTACW	2 cut(s)	497, 1050
TauI	GCSGC	1 cut(s)	1815
TfiI	GAWTC	1 cut(s)	419
Tru1I	TTAA	7 cut(s)	195, 215, 527, 672, 770, 1146, 1242
Tru9I	TTAA	7 cut(s)	195, 215, 527, 672, 770, 1146, 1242
TscAI	CASTG	3 cut(s)	44, 100, 660
TseFI	GTSAC	6 cut(s)	135, 223, 470, 593, 811, 1184
TseI	GCWGC	5 cut(s)	620, 1229, 1289, 1788, 1930
Tsp45I	GTSAC	6 cut(s)	135, 223, 470, 593, 811, 1184
TspDTI	ATGAA	9 cut(s)	120, 219, 605, 679, 1063, 1104, 1487, 1878, 1893
TspGWI	ACGGA	3 cut(s)	791, 1304, 1882
TspRI	CASTG	3 cut(s)	44, 100, 660
Vha464I	CTTAAG	3 cut(s)	214, 671, 1241
VpaK11BI	GGWCC	2 cut(s)	506, 1450
VspI	ATTAAT	1 cut(s)	527
XapI	RAATTY	2 cut(s)	932, 1560
XhoI	CTCGAG	3 cut(s)	996, 1538, 1736
XmiI	GTMKAC	1 cut(s)	13
XmnI	GAANNNNTTC	1 cut(s)	936
XspI	CTAG	5 cut(s)	578, 1407, 1430, 1482, 1800
ZraI	GACGTC	1 cut(s)	55
Zsp2I	ATGCAT	3 cut(s)	116, 1009, 1133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.