Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005148

Physical Location & Seq

Genomic Coordinates Forward (+)

2478220 .. 2482336

Gene Structure 4117 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1034300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGACAACTTGTATACCCGCCGGAAGTGGTAGACTCCATTGTAGAGTGAGGGACATCAAGGGACAAGGTTGTAGAAGGACTTCTTGGATTTCCAATGTTACATTTAGTGGATGCATACGGAAAGACCTTCCATGGTCACACGGGGTATCAAAGTTCTTGCAGATCCAAAGATGTAACTTTTCCCAATCACCAATCACTGAGAACTCAAAGACCTTAAGAACAATCACTTCATCAAGCTTGAGAGATGGTTCCACTAAGTTTTTTGATTTTGCTGTTATTGGTAGTGGAGTTGCTGGGCTCCGCTATGCCCTTGAAGTGGCAAAACATGGATCCGTTGCTGTGATTACCAAAGCTGAACCTCATGAGAGCAATACAAACTATGCTCAAGGTGGTGTTAGTGCTGTTCTTTCCCAATCGGATTCTGTAGAGAGTCATATGCAGGACACCATTGTGGCAGGTGCTTATCTCTGCGACGAGGAGACTGTCAGAGTTGTCTGTACTGAAGGACCCGAAAGAATTAGAGAACTGATTGCAATGGGGGCATCCTTTGATCATGGGGAGGATGGAAACTTACACCTAGCAAGAGAGGGAGGTCACTCTCATCGCAGGATTGTTCATGCTGCTGATATGACTGGAAGGGAGATTGAACGGGCTCTGTTGAAGGCAGCTCTTAAGGATCCTAATATCTTCATGTTTGAACACCATCTTGCGATAGATTTGCTAACTTGCCAGGATGGTTCCGATACAGTTTGTCTTGGAGTTGACACTTTAAATACAGAAACACAAGAGGTGATACGGTTTATCTCAAAGGTGACTTTACTTGCGTCAGGTGGGGCTGGACAAATCTATCCGACAACTACAAATCCTCCGGTAGCCACAGGGGATGGAATTGCGATGGCCCATCGAGCTCAAGCTGTGATATCCAACATGGAATTTGTTCAGTTCCATCCAACGGCATTAGCCGATGAAGGCCTTCCCATCCAACCATCCAAGGCTCGAGAAAATGCATTTCTCATCACTGAAGCTGTCAGAGGTGATGGAGCCATCCTGTACAATCTAGACATGGAAAGGTTTATGCCACTCTATGATGAACGAGCAGAACTTGCTCCGAGGGATGTGGTGGCCAGAGGCATAGACGACCAGCTTAAAAAGCGAAATGAGAAGTATGTGCTACTTGATATAAGTCACAAGCCCAGAGAAAAAATCCTCTCACACTTCCCCAACATAGCAGCCGAGTGCCTTAAGTACGCCTTGGACATAACTTGCCAGCCAATTCCCGTTGTTCCTGCTGCTCACTATATGTGTGGGGGAGTCCGTGCTGGACTCCAGGGAGAGACAAATGTGCAGGGCCTGTACGTGGCAGGCGAAGTTGCTTGCACAGGTTTGCATGGAGCCAACCGTCTTGCTAGTAACTCATTGCTTGAAGCACTAGTTTTCGCACGAAGAGCAGTCCAGCCCTCAATTAAACACATGAAGTGCTCTAGCCTGGACTTCAGTGCTTCAGATTGGTGGGCCAGACCAGTCGTGCCTGTCTCACTCGAGAGCAATGCCATCGAAAAAATTTTGTCAATGACAAGGGAAGTAAGAAAAGAAGTGCAATCGATCATGTGGAAATATGTTGGAATTGTACGTTCTACAACGAGGCTTGAAACAGCAAAGCAAAAGATTTGTGATCTTGAGGATAAGTGGGAAGACTACTTATTTCAGATGGGATGGGAACCGACGATGGTGGGGCTTGAGACTTGCGAAATGAGAAACTTCTTCTGTTGTGCAAAACTGGTGGTGAGCAGCGCCCTTGCTAGGCATGAGAGCCGCGGACTTCACTACACCATCGATTTTCCTGATCTTGAGGAAAGTAAGAGGCTTCCAACAGTTATATTCCCTTGTGCATCTGTACGAAGTTCATGGAGTTCGCGTCAGCTACAGAAGCTGCCATTGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009435 GO:0009507 GO:0009536 GO:0009987 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019637 GO:0019674 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046496 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072524 GO:0072525 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00250 ko00760 ko01100 map00250 map00760 map01100

Pfam Domains

Protein Families

FAD_binding_2 Succ_DH_flav_C

Protein Analysis

648

Amino Acids

71.44

Weight (kDa)

6.61

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_2	PF00890	89 - 473	2.5e-89	FAD binding domain
DAO	PF01266	89 - 205	2.4e-08	FAD dependent oxidoreductase
Succ_DH_flav_C	PF02910	529 - 617	2.7e-16	Fumarate reductase flavoprotein C-term

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14760
fragaria_vesca	FvH4_6g50750 FvH4_6g50750 FvH4_6g50750 FvH4_6g50750 FvH4_6g50750
malus_domestica	MD09G1032700.v1.1 MD17G1034300.v1.1
prunus_persica	Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1 Prupe.3G285300_v2.0.a1
pyrus_communis	pycom111g02610 pycom17g03170
rosa_chinensis	RchiOBHm_Chr2g0171691
rosa_laevigata	RLG00000022029
rosa_multiflora	Rmu_ssc0000252.1_g000010
rosa_roxburghii	Rroxscaffold_2G00080520
rosa_rugosa	Rorug02G0555400
rosa_samantha	Rh2AG630400 Rh2BG640900 Rh2BG643400 Rh2CG609100 Rh2DG651800
rosa_wichuraiana	Rw2G052170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	446
Acc36I	ACCTGC	1 cut(s)	446
AccI	GTMKAC	2 cut(s)	13, 31
AccII	CGCG	2 cut(s)	1815, 1915
AciI	CCGC	4 cut(s)	18, 301, 1813, 1815
AclWI	GGATC	5 cut(s)	157, 324, 337, 671, 684
AcoI	YGGCCR	1 cut(s)	1122
AcsI	RAATTY	2 cut(s)	932, 1560
AcuI	CTGAAG	4 cut(s)	522, 1041, 1477, 1485
AfaI	GTAC	6 cut(s)	499, 1052, 1247, 1355, 1629, 1896
AfiI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
AflII	CTTAAG	3 cut(s)	214, 671, 1241
AgsI	TTSAA	6 cut(s)	314, 647, 661, 698, 1424, 1649
AhlI	ACTAGT	1 cut(s)	1429
AjnI	CCWGG	3 cut(s)	729, 1326, 1485
AjuI	GAANNNNNNNTTGG	2 cut(s)	67, 99
AleI	CACNNNNGTG	1 cut(s)	449
AluBI	AGCT	9 cut(s)	237, 353, 668, 908, 914, 1025, 1144, 1921, 1930
AluI	AGCT	9 cut(s)	237, 353, 668, 908, 914, 1025, 1144, 1921, 1930
Alw21I	GWGCWC	2 cut(s)	910, 1481
Alw26I	GTCTC	4 cut(s)	473, 1328, 1537, 1733
AlwI	GGATC	5 cut(s)	157, 324, 337, 671, 684
AlwNI	CAGNNNCTG	2 cut(s)	1351, 1930
Ama87I	CYCGRG	2 cut(s)	996, 1538
AoxI	GGCC	5 cut(s)	897, 970, 1122, 1348, 1512
ApeKI	GCWGC	6 cut(s)	620, 665, 1229, 1289, 1788, 1930
ApoI	RAATTY	2 cut(s)	932, 1560
Asp700I	GAANNNNTTC	3 cut(s)	79, 936, 972
AspLEI	GCGC	1 cut(s)	1793
AspS9I	GGNCC	4 cut(s)	506, 898, 1348, 1512
AsuHPI	GGTGA	5 cut(s)	180, 802, 823, 1046, 1795
AvaI	CYCGRG	2 cut(s)	996, 1538
AvaII	GGWCC	1 cut(s)	506
BalI	TGGCCA	1 cut(s)	1124
BamHI	GGATCC	2 cut(s)	329, 676
BanII	GRGCYC	3 cut(s)	300, 655, 910
BarI	GAAGNNNNNNTAC	2 cut(s)	64, 96
BbsI	GAAGAC	1 cut(s)	1698
Bbv12I	GWGCWC	2 cut(s)	910, 1481
BbvI	GCAGC	6 cut(s)	607, 677, 1241, 1276, 1800, 1917
BceAI	ACGGC	1 cut(s)	969
BcgI	CGANNNNNNTGC	6 cut(s)	700, 734, 1529, 1534, 1563, 1568
BciT130I	CCWGG	3 cut(s)	731, 1328, 1487
BclI	TGATCA	1 cut(s)	550
BcoDI	GTCTC	4 cut(s)	473, 1328, 1537, 1733
BcuI	ACTAGT	1 cut(s)	1429
BfaI	CTAG	7 cut(s)	578, 1058, 1407, 1430, 1482, 1800, 1942
BfmI	CTRYAG	2 cut(s)	423, 1922
BfoI	RGCGCY	1 cut(s)	1794
BfrI	CTTAAG	3 cut(s)	214, 671, 1241
BfuAI	ACCTGC	1 cut(s)	446
BisI	GCNGC	7 cut(s)	621, 666, 1230, 1290, 1789, 1813, 1931
BlsI	GCNGC	7 cut(s)	622, 667, 1231, 1291, 1790, 1814, 1932
Bme1390I	CCNGG	3 cut(s)	731, 1328, 1487
Bme18I	GGWCC	1 cut(s)	506
BmeT110I	CYCGRG	2 cut(s)	996, 1538
BmgT120I	GGNCC	4 cut(s)	506, 898, 1348, 1512
BmiI	GGNNCC	9 cut(s)	250, 299, 331, 508, 678, 739, 1042, 1393, 1719
BmrFI	CCNGG	3 cut(s)	731, 1328, 1487
BmsI	GCATC	3 cut(s)	101, 551, 1898
BpiI	GAAGAC	1 cut(s)	1698
BpmI	CTGGAG	1 cut(s)	1310
BpuEI	CTTGAG	6 cut(s)	259, 369, 894, 1697, 1757, 1868
Bsa29I	ATCGAT	2 cut(s)	1601, 1833
BsaAI	YACGTR	1 cut(s)	1357
BsaJI	CCNNGG	6 cut(s)	131, 990, 1109, 1251, 1327, 1813
BsaWI	WCCGGW	1 cut(s)	868
BsaXI	ACNNNNNCTCC	4 cut(s)	850, 880, 1900, 1930
Bsc4I	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
Bse1I	ACTGG	3 cut(s)	637, 1520, 1782
Bse3DI	GCAATG	3 cut(s)	540, 1415, 1552
BseBI	CCWGG	3 cut(s)	731, 1328, 1487
BseCI	ATCGAT	2 cut(s)	1601, 1833
BseDI	CCNNGG	6 cut(s)	131, 990, 1109, 1251, 1327, 1813
BseLI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
BseMI	GCAATG	3 cut(s)	540, 1415, 1552
BseMII	CTCAG	1 cut(s)	189
BseNI	ACTGG	3 cut(s)	637, 1520, 1782
BseRI	GAGGAG	1 cut(s)	491
BseXI	GCAGC	6 cut(s)	607, 677, 1241, 1276, 1800, 1917
BseYI	CCCAGC	1 cut(s)	293
BsgI	GTGCAG	1 cut(s)	1364
Bsh1236I	CGCG	2 cut(s)	1815, 1915
BshFI	GGCC	5 cut(s)	899, 972, 1124, 1350, 1514
BshVI	ATCGAT	2 cut(s)	1601, 1833
BsiHKAI	GWGCWC	2 cut(s)	910, 1481
BsiHKCI	CYCGRG	2 cut(s)	996, 1538
BsiSI	CCGG	2 cut(s)	21, 869
BslFI	GGGAC	2 cut(s)	65, 75
BslI	CCNNNNNNNGG	4 cut(s)	316, 952, 1357, 1800
BsmAI	GTCTC	4 cut(s)	473, 1328, 1537, 1733
BsmFI	GGGAC	2 cut(s)	65, 75
BsnI	GGCC	5 cut(s)	899, 972, 1124, 1350, 1514
BsoBI	CYCGRG	2 cut(s)	996, 1538
Bsp1286I	GDGCHC	4 cut(s)	300, 655, 910, 1481
Bsp1407I	TGTACA	1 cut(s)	1050
Bsp143I	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
Bsp19I	CCATGG	1 cut(s)	131
BspACI	CCGC	4 cut(s)	18, 301, 1813, 1815
BspANI	GGCC	5 cut(s)	899, 972, 1124, 1350, 1514
BspCNI	CTCAG	1 cut(s)	190
BspDI	ATCGAT	2 cut(s)	1601, 1833
BspFNI	CGCG	2 cut(s)	1815, 1915
BspHI	TCATGA	1 cut(s)	361
BspLI	GGNNCC	9 cut(s)	250, 299, 331, 508, 678, 739, 1042, 1393, 1719
BspMI	ACCTGC	1 cut(s)	446
BspPI	GGATC	5 cut(s)	157, 324, 337, 671, 684
BspQI	GCTCTTC	1 cut(s)	1438
BspTI	CTTAAG	3 cut(s)	214, 671, 1241
BsrDI	GCAATG	3 cut(s)	540, 1415, 1552
BsrGI	TGTACA	1 cut(s)	1050
BsrI	ACTGG	3 cut(s)	637, 1520, 1782
BssECI	CCNNGG	6 cut(s)	131, 990, 1109, 1251, 1327, 1813
BssMI	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
BssNAI	GTATAC	1 cut(s)	14
BssT1I	CCWWGG	3 cut(s)	131, 990, 1251
Bst1107I	GTATAC	1 cut(s)	14
Bst2UI	CCWGG	3 cut(s)	731, 1328, 1487
Bst4CI	ACNGT	5 cut(s)	484, 748, 798, 1400, 1873
Bst6I	CTCTTC	1 cut(s)	1438
BstAFI	CTTAAG	3 cut(s)	214, 671, 1241
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	1 cut(s)	1050
BstBAI	YACGTR	1 cut(s)	1357
BstC8I	GCNNGC	3 cut(s)	1268, 1363, 1375
BstDEI	CTNAG	2 cut(s)	198, 255
BstDSI	CCRYGG	2 cut(s)	131, 1813
BstFNI	CGCG	2 cut(s)	1815, 1915
BstH2I	RGCGCY	1 cut(s)	1794
BstHHI	GCGC	1 cut(s)	1793
BstKTI	GATC	7 cut(s)	165, 332, 553, 679, 1605, 1675, 1846
BstMAI	GTCTC	4 cut(s)	473, 1328, 1537, 1733
BstMBI	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
BstMWI	GCNNNNNNNGC	6 cut(s)	539, 905, 1103, 1150, 1445, 1927
BstNI	CCWGG	3 cut(s)	731, 1328, 1487
BstSCI	CCNGG	3 cut(s)	729, 1326, 1485
BstSFI	CTRYAG	2 cut(s)	423, 1922
BstUI	CGCG	2 cut(s)	1815, 1915
BstV1I	GCAGC	6 cut(s)	607, 677, 1241, 1276, 1800, 1917
BstV2I	GAAGAC	1 cut(s)	1698
BstX2I	RGATCY	3 cut(s)	162, 329, 676
BstYI	RGATCY	3 cut(s)	162, 329, 676
BstZ17I	GTATAC	1 cut(s)	14
Bsu15I	ATCGAT	2 cut(s)	1601, 1833
BsuRI	GGCC	5 cut(s)	899, 972, 1124, 1350, 1514
BsuTUI	ATCGAT	2 cut(s)	1601, 1833
BtgI	CCRYGG	2 cut(s)	131, 1813
BtgZI	GCGATG	2 cut(s)	587, 908
BtsIMutI	CAGTG	3 cut(s)	195, 1017, 1501
BveI	ACCTGC	1 cut(s)	446
Cac8I	GCNNGC	3 cut(s)	1268, 1363, 1375
CaiI	CAGNNNCTG	2 cut(s)	1351, 1930
CciI	TCATGA	1 cut(s)	361
CfoI	GCGC	1 cut(s)	1793
Cfr13I	GGNCC	4 cut(s)	506, 898, 1348, 1512
Cfr42I	CCGCGG	1 cut(s)	1816
ClaI	ATCGAT	2 cut(s)	1601, 1833
CseI	GACGC	2 cut(s)	813, 1904
Csp6I	GTAC	6 cut(s)	498, 1051, 1246, 1354, 1628, 1895
CspCI	CAANNNNNGTGG	2 cut(s)	1761, 1796
CviQI	GTAC	6 cut(s)	498, 1051, 1246, 1354, 1628, 1895
DdeI	CTNAG	2 cut(s)	198, 255
DpnI	GATC	7 cut(s)	164, 331, 552, 678, 1604, 1674, 1845
DpnII	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
DraI	TTTAAA	1 cut(s)	771
EaeI	YGGCCR	1 cut(s)	1122
Eam1104I	CTCTTC	1 cut(s)	1438
EarI	CTCTTC	1 cut(s)	1438
Ecl136II	GAGCTC	1 cut(s)	908
Eco130I	CCWWGG	3 cut(s)	131, 990, 1251
Eco147I	AGGCCT	1 cut(s)	972
Eco24I	GRGCYC	3 cut(s)	300, 655, 910
Eco32I	GATATC	1 cut(s)	921
Eco47I	GGWCC	1 cut(s)	506
Eco53kI	GAGCTC	1 cut(s)	908
Eco57I	CTGAAG	4 cut(s)	522, 1041, 1477, 1485
Eco88I	CYCGRG	2 cut(s)	996, 1538
EcoICRI	GAGCTC	1 cut(s)	908
EcoO109I	RGGNCCY	2 cut(s)	506, 1348
EcoRII	CCWGG	3 cut(s)	729, 1326, 1485
EcoRV	GATATC	1 cut(s)	921
EcoT14I	CCWWGG	3 cut(s)	131, 990, 1251
EcoT22I	ATGCAT	2 cut(s)	116, 1009
EcoT38I	GRGCYC	3 cut(s)	300, 655, 910
ErhI	CCWWGG	3 cut(s)	131, 990, 1251
FalI	AAGNNNNNCTT	4 cut(s)	67, 99, 1235, 1267
FaqI	GGGAC	2 cut(s)	65, 75
FauI	CCCGC	1 cut(s)	25
FauNDI	CATATG	1 cut(s)	435
FbaI	TGATCA	1 cut(s)	550
FblI	GTMKAC	2 cut(s)	13, 31
Fnu4HI	GCNGC	7 cut(s)	621, 666, 1230, 1290, 1789, 1813, 1931
FriOI	GRGCYC	3 cut(s)	300, 655, 910
Fsp4HI	GCNGC	7 cut(s)	621, 666, 1230, 1290, 1789, 1813, 1931
FspBI	CTAG	7 cut(s)	578, 1058, 1407, 1430, 1482, 1800, 1942
GlaI	GCGC	1 cut(s)	1792
GluI	GCNGC	7 cut(s)	621, 666, 1230, 1290, 1789, 1813, 1931
GsaI	CCCAGC	1 cut(s)	297
GsuI	CTGGAG	1 cut(s)	1310
HaeII	RGCGCY	1 cut(s)	1794
HaeIII	GGCC	5 cut(s)	899, 972, 1124, 1350, 1514
HapII	CCGG	2 cut(s)	21, 869
HgaI	GACGC	2 cut(s)	813, 1904
HhaI	GCGC	1 cut(s)	1793
Hin6I	GCGC	1 cut(s)	1791
HinP1I	GCGC	1 cut(s)	1791
HincII	GTYRAC	1 cut(s)	763
HindII	GTYRAC	1 cut(s)	763
HindIII	AAGCTT	1 cut(s)	235
HinfI	GANTC	5 cut(s)	33, 419, 430, 1311, 1323
HpaII	CCGG	2 cut(s)	21, 869
HphI	GGTGA	5 cut(s)	180, 802, 823, 1046, 1795
Hpy166II	GTNNAC	3 cut(s)	14, 32, 763
Hpy188I	TCNGA	8 cut(s)	418, 488, 742, 852, 1031, 1110, 1504, 1707
Hpy188III	TCNNGA	7 cut(s)	362, 998, 1058, 1540, 1676, 1841, 1847
Hpy8I	GTNNAC	3 cut(s)	14, 32, 763
Hpy99I	CGWCG	2 cut(s)	476, 1726
HpyAV	CCTTC	7 cut(s)	69, 137, 497, 630, 655, 962, 983
HpyCH4III	ACNGT	5 cut(s)	484, 748, 798, 1400, 1873
HpyCH4IV	ACGT	2 cut(s)	1356, 1630
HpyF10VI	GCNNNNNNNGC	6 cut(s)	539, 905, 1103, 1150, 1445, 1927
HpyF3I	CTNAG	2 cut(s)	198, 255
HpySE526I	ACGT	2 cut(s)	1356, 1630
HspAI	GCGC	1 cut(s)	1791
Ksp22I	TGATCA	1 cut(s)	550
KspI	CCGCGG	1 cut(s)	1816
Kzo9I	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
LguI	GCTCTTC	1 cut(s)	1438
LmnI	GCTCC	4 cut(s)	303, 1040, 1111, 1391
Lsp1109I	GCAGC	6 cut(s)	607, 677, 1241, 1276, 1800, 1917
LweI	GCATC	3 cut(s)	101, 551, 1898
MaeI	CTAG	7 cut(s)	578, 1058, 1407, 1430, 1482, 1800, 1942
MaeII	ACGT	2 cut(s)	1356, 1630
MaeIII	GTNAC	7 cut(s)	97, 135, 173, 593, 811, 1184, 1409
MalI	GATC	7 cut(s)	164, 331, 552, 678, 1604, 1674, 1845
MboI	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
MboII	GAAGA	4 cut(s)	679, 1455, 1703, 1753
MflI	RGATCY	3 cut(s)	162, 329, 676
MhlI	GDGCHC	4 cut(s)	300, 655, 910, 1481
MlsI	TGGCCA	1 cut(s)	1124
MluCI	AATT	7 cut(s)	516, 888, 932, 1272, 1461, 1560, 1623
MluNI	TGGCCA	1 cut(s)	1124
MlyI	GAGTC	4 cut(s)	27, 439, 1317, 1320
MmeI	TCCRAC	6 cut(s)	875, 948, 974, 1006, 1600, 1892
Mox20I	TGGCCA	1 cut(s)	1124
Mph1103I	ATGCAT	2 cut(s)	116, 1009
MroXI	GAANNNNTTC	3 cut(s)	79, 936, 972
MscI	TGGCCA	1 cut(s)	1124
MseI	TTAA	6 cut(s)	215, 672, 770, 1146, 1242, 1464
MslI	CAYNNNNRTG	1 cut(s)	449
Msp20I	TGGCCA	1 cut(s)	1124
MspA1I	CMGCKG	1 cut(s)	1815
MspCI	CTTAAG	3 cut(s)	214, 671, 1241
MspI	CCGG	2 cut(s)	21, 869
MspR9I	CCNGG	3 cut(s)	731, 1328, 1487
MvaI	CCWGG	3 cut(s)	731, 1328, 1487
MvnI	CGCG	2 cut(s)	1815, 1915
MwoI	GCNNNNNNNGC	6 cut(s)	539, 905, 1103, 1150, 1445, 1927
NcoI	CCATGG	1 cut(s)	131
NdeI	CATATG	1 cut(s)	435
NdeII	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
NlaIV	GGNNCC	9 cut(s)	250, 299, 331, 508, 678, 739, 1042, 1393, 1719
NmeAIII	GCCGAG	1 cut(s)	1258
NmuCI	GTSAC	4 cut(s)	135, 593, 811, 1184
NsiI	ATGCAT	2 cut(s)	116, 1009
OliI	CACNNNNGTG	1 cut(s)	449
PaeR7I	CTCGAG	2 cut(s)	996, 1538
PagI	TCATGA	1 cut(s)	361
PaqCI	CACCTGC	1 cut(s)	446
PceI	AGGCCT	1 cut(s)	972
PciSI	GCTCTTC	1 cut(s)	1438
PcsI	WCGNNNNNNNCGW	1 cut(s)	1362
PdmI	GAANNNNTTC	3 cut(s)	79, 936, 972
PfeI	GAWTC	1 cut(s)	419
PkrI	GCNGC	7 cut(s)	622, 667, 1231, 1291, 1790, 1814, 1932
PleI	GAGTC	4 cut(s)	27, 438, 1317, 1319
PpsI	GAGTC	4 cut(s)	27, 438, 1317, 1319
Ppu21I	YACGTR	1 cut(s)	1357
PpuMI	RGGWCCY	1 cut(s)	506
Psp124BI	GAGCTC	1 cut(s)	910
Psp5II	RGGWCCY	1 cut(s)	506
Psp6I	CCWGG	3 cut(s)	729, 1326, 1485
PspFI	CCCAGC	1 cut(s)	293
PspGI	CCWGG	3 cut(s)	729, 1326, 1485
PspN4I	GGNNCC	9 cut(s)	250, 299, 331, 508, 678, 739, 1042, 1393, 1719
PspPI	GGNCC	4 cut(s)	506, 898, 1348, 1512
PspPPI	RGGWCCY	1 cut(s)	506
PstNI	CAGNNNCTG	2 cut(s)	1351, 1930
PsuI	RGATCY	3 cut(s)	162, 329, 676
RsaI	GTAC	6 cut(s)	499, 1052, 1247, 1355, 1629, 1896
RsaNI	GTAC	6 cut(s)	498, 1051, 1246, 1354, 1628, 1895
RseI	CAYNNNNRTG	1 cut(s)	449
SacI	GAGCTC	1 cut(s)	910
SacII	CCGCGG	1 cut(s)	1816
SapI	GCTCTTC	1 cut(s)	1438
SaqAI	TTAA	6 cut(s)	215, 672, 770, 1146, 1242, 1464
SatI	GCNGC	7 cut(s)	621, 666, 1230, 1290, 1789, 1813, 1931
Sau3AI	GATC	7 cut(s)	162, 329, 550, 676, 1602, 1672, 1843
Sau96I	GGNCC	4 cut(s)	506, 898, 1348, 1512
SchI	GAGTC	4 cut(s)	27, 439, 1317, 1320
ScrFI	CCNGG	3 cut(s)	731, 1328, 1487
SduI	GDGCHC	4 cut(s)	300, 655, 910, 1481
SfaNI	GCATC	3 cut(s)	101, 551, 1898
SfcI	CTRYAG	2 cut(s)	423, 1922
Sfr274I	CTCGAG	2 cut(s)	996, 1538
Sfr303I	CCGCGG	1 cut(s)	1816
SgrBI	CCGCGG	1 cut(s)	1816
SinI	GGWCC	1 cut(s)	506
SlaI	CTCGAG	2 cut(s)	996, 1538
SmiMI	CAYNNNNRTG	1 cut(s)	449
SpeI	ACTAGT	1 cut(s)	1429
Sse9I	AATT	7 cut(s)	516, 888, 932, 1272, 1461, 1560, 1623
SseBI	AGGCCT	1 cut(s)	972
SsiI	CCGC	4 cut(s)	18, 301, 1813, 1815
SspMI	CTAG	7 cut(s)	578, 1058, 1407, 1430, 1482, 1800, 1942
SstI	GAGCTC	1 cut(s)	910
StuI	AGGCCT	1 cut(s)	972
StyD4I	CCNGG	3 cut(s)	729, 1326, 1485
StyI	CCWWGG	3 cut(s)	131, 990, 1251
TaaI	ACNGT	5 cut(s)	484, 748, 798, 1400, 1873
TaiI	ACGT	2 cut(s)	1359, 1633
TaqI	TCGA	6 cut(s)	904, 997, 1539, 1554, 1601, 1833
TasI	AATT	7 cut(s)	516, 888, 932, 1272, 1461, 1560, 1623
TatI	WGTACW	2 cut(s)	497, 1050
TauI	GCSGC	1 cut(s)	1815
TfiI	GAWTC	1 cut(s)	419
Tru1I	TTAA	6 cut(s)	215, 672, 770, 1146, 1242, 1464
Tru9I	TTAA	6 cut(s)	215, 672, 770, 1146, 1242, 1464
TscAI	CASTG	3 cut(s)	202, 1024, 1501
TseFI	GTSAC	4 cut(s)	135, 593, 811, 1184
TseI	GCWGC	6 cut(s)	620, 665, 1229, 1289, 1788, 1930
Tsp45I	GTSAC	4 cut(s)	135, 593, 811, 1184
TspDTI	ATGAA	7 cut(s)	219, 605, 679, 981, 1104, 1487, 1893
TspGWI	ACGGA	3 cut(s)	133, 322, 1304
TspRI	CASTG	3 cut(s)	202, 1024, 1501
Vha464I	CTTAAG	3 cut(s)	214, 671, 1241
VpaK11BI	GGWCC	1 cut(s)	506
XapI	RAATTY	2 cut(s)	932, 1560
XbaI	TCTAGA	1 cut(s)	1057
XhoI	CTCGAG	2 cut(s)	996, 1538
XmiI	GTMKAC	2 cut(s)	13, 31
XmnI	GAANNNNTTC	3 cut(s)	79, 936, 972
XspI	CTAG	7 cut(s)	578, 1058, 1407, 1430, 1482, 1800, 1942
Zsp2I	ATGCAT	2 cut(s)	116, 1009

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.