Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0016495

Physical Location & Seq

Genomic Coordinates Reverse (-)

2184445 .. 2188438

Gene Structure 3994 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1035800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGAATAGAATAATCCCCACGCCGGCCGCTAAATTCCGCCCCCTCTACACCGACCCTAACTCCGCCGACGTCTCCTCCTCCGCCGTGCCGGACGAACAGAACCCGCTCACAATCCTATGGAACCACCAAATCCTCGACCCCGACAGCGATATCGTAGCGCATTGGAACCACATCTTCCTCGTCATCTGCATTCTGGCCCTCTTCGTCGACCCCCTCTATTTCTACCTCCCTTGGGTCGGCGACACAGCTTGCTTGACCACCGACAACCAGCTCGCCGTCGTCGTCACCTGCTTCCGCACCCTCACCGACCTCTTCTACCTCCTCCACATGATCATCAAGTTCCGCACCGCCTATGTCAATCCCAGCACTAGGGTTTTCGGCCGCGGTGACCTCGTCACGGACCCCCACCTGATTGCCATCCGCTACTTGAAGTCCGACTTCATTGTCGATCTTGCCGCTACCCTTCCGGTCCCCCAGCTTGTAATCTGGTTGGTGATTCCAGCAACGAGGAACTCGAGAGCTGATCATGCCAACAACACTCTGGCACTGTTCGTTTTGATTCAGTACATTCCTCGGATCTTCCTCATCTTCCCGTTGAATCAACGTATCATAAAGACAACTGGTGTCGTAGCAAAGACTGCATGGGCGGGAGCTGCGTACAATCTCCTCCTCTTCGTTCTAGCCGGCCATATAGTTGGTTCTGTCTGGTATATATCGTCCATAGGGCGGCAGTTCTCTTGCTGGAAATCCTATTGTAAAGAGGAGAACTCGAAAAGGATTGTTTCTTGCCTCACAAGTTTTCTAGATTGTAGTAGTACAGAACTACCAGAACGGAAGTACTGGGTCAACGTCACCGAAGTTGTTAAAAGTTGTGATGCAAATAATGAGAAAGCCAAATTTAAGTTTGGAATTTTTGGGGATGCTTTCAAGAGTGATGTTGCTACTACAAAGGACTTCTATCCAAAGTACCTGTATTGTTTGTGGTGGGGTTTAAGAAACTTAAGTTCATATGGGCAGACTCTGAAGACGAGCACGTACTTTTGGGAGATAATGTTTTCCATTACACTATGTCTCACTGGTCTAATTCTGTTCTCATTATTGATTGGCAACATGCAGACTTATCTGCAATCAATGTCGATAAAATTCGAAGAATGGAGGATTAAGCTAAAAGATACTGAGGAGTGGATGAGGCATCGGCAGTTACCTGAGGATTTGCGCGAACGTGTTCGTAGATTTATGCAGTACAAATGGTTTGCCACAAGAGGAGTTGATGAAGAATCCATATTGCGGTCATTACCTTTAGACCTCTGCCATGAAATCCAGCGGCACTTATGCCTTTCGCTTGTTCGTCGTGTTCCTTTTTTTTCACAAATGGATGATCAGCTTCTCGATGCCATATGCGAACGTCTTGTCTCATCCTTGAGTATCCAAGGCACACATATAGTTCAAGAGGGTGATCCAGTAAATGAGATGTTGTTTATCATTCGAGGGAAACTCGAGAGCTCCACAACCAATGGAGGGAGGTCCGGGTTCTTTAACTCCATCACTCTTGGACCTGGTGACTTCTGCGGCGAGGAACTGCTGACATGGGCCTTGATGCCAAGCTCTAGTCTCAACTTGCCTTCTTCCACGCGGACAGTCAGAGCTCTTACCGAAGTTGAAGCTTTTGCACTTCGAGCAGAGGACCTTAAATTCGTTGCTGGGCAATTCAAACGCCTTCACAGCAAGAAACTACAGCATGCCTTTAGGTACTATTCCCACCAATGGAGGACATGGGGTTCTTGCTTTATCCAAGCCGCTTGGAGAAGGTTTAAGAAGCGAAAGATGACGAGGGACTTGGCTATGCAGGAGAGCTTGTACTATATGCAAAATCCAGGCCAAGAGGAAGACGGTTACTACTACTATGACGAAGAACAAGCAGGTGGAAATTATGAGGGTGATGCGAGTGGTGAGAGATCAGGAGAGAATGCAAGCAATTTGGGCCAAGGCCAACATCTTGGGGTCACATTTCTCGCTTCCAAGTTTGCTGCAACTACAAGGCGAGGAATTGCGCAGAAAGCTCAGGTGGTTGAAGCTGCTTCTCCCAGTTTGGACATGCCTCGGTTGTTAAAGCCAGATGAACCTGATTTCTCTGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0008324 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016324 GO:0019725 GO:0022414 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0034220 GO:0040007 GO:0042391 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0046873 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051704 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072511 GO:0098590 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

714

Amino Acids

81.57

Weight (kDa)

7.83

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	55 - 378	2.2e-17	Ion transport protein
cNMP_binding	PF00027	478 - 564	9.7e-09	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14870
fragaria_vesca	FvH4_6g50510
malus_domestica	MD09G1035800.v1.1 MD17G1037400.v1.1
prunus_persica	Prupe.3G282200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0170931
rosa_laevigata	RLG00000021993
rosa_multiflora	Rmu_sc0025148.1_g000002
rosa_roxburghii	Rroxscaffold_2G00080970
rosa_rugosa	Rorug02G0552400
rosa_samantha	Rh2BG636000 Rh2CG604500 Rh2DG647600
rosa_wichuraiana	Rw2G051780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	296, 1910
AasI	GACNNNNNNGTC	1 cut(s)	443
AatII	GACGTC	1 cut(s)	72
Acc16I	TGCGCA	1 cut(s)	2052
Acc36I	ACCTGC	2 cut(s)	296, 1910
AccBSI	CCGCTC	1 cut(s)	106
AccI	GTMKAC	1 cut(s)	207
AccII	CGCG	3 cut(s)	384, 1218, 1633
AclWI	GGATC	2 cut(s)	584, 1451
AcoI	YGGCCR	3 cut(s)	24, 379, 685
AcsI	RAATTY	5 cut(s)	32, 896, 909, 1142, 1691
AcuI	CTGAAG	1 cut(s)	1043
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	9 cut(s)	566, 659, 817, 839, 968, 1037, 1244, 1751, 1859
AfiI	CCNNNNNNNGG	9 cut(s)	22, 232, 236, 369, 397, 726, 1287, 1518, 1764
AflII	CTTAAG	1 cut(s)	1000
AflIII	ACRYGT	1 cut(s)	1222
AgsI	TTSAA	7 cut(s)	430, 598, 928, 1448, 1661, 1711, 2072
AjnI	CCWGG	2 cut(s)	1555, 1873
AloI	GAACNNNNNNTCC	2 cut(s)	1762, 1794
Alw21I	GWGCWC	3 cut(s)	1034, 1505, 1648
Alw26I	GTCTC	4 cut(s)	76, 1076, 1417, 1616
AlwI	GGATC	2 cut(s)	584, 1451
AlwNI	CAGNNNCTG	1 cut(s)	1021
Ama87I	CYCGRG	2 cut(s)	514, 1496
AoxI	GGCC	8 cut(s)	24, 195, 379, 685, 1590, 1876, 1981, 1987
ApeKI	GCWGC	3 cut(s)	653, 2027, 2075
ApoI	RAATTY	5 cut(s)	32, 896, 909, 1142, 1691
ArsI	GACNNNNNNTTYG	4 cut(s)	1395, 1427, 1676, 1708
Asp700I	GAANNNNTTC	1 cut(s)	1224
AspLEI	GCGC	3 cut(s)	160, 1218, 2053
AspS9I	GGNCC	8 cut(s)	196, 400, 469, 1524, 1553, 1590, 1684, 1981
AsuC2I	CCSGG	1 cut(s)	1528
AsuHPI	GGTGA	9 cut(s)	277, 295, 398, 505, 844, 1466, 1571, 1949, 1961
AsuII	TTCGAA	1 cut(s)	1146
AvaI	CYCGRG	2 cut(s)	514, 1496
AvaII	GGWCC	5 cut(s)	400, 469, 1524, 1553, 1684
AxyI	CCTNAGG	1 cut(s)	1206
BanII	GRGCYC	2 cut(s)	1505, 1648
BbsI	GAAGAC	2 cut(s)	1031, 1893
Bbv12I	GWGCWC	3 cut(s)	1034, 1505, 1648
BbvI	GCAGC	3 cut(s)	640, 2014, 2062
BccI	CCATC	2 cut(s)	425, 1550
BceAI	ACGGC	2 cut(s)	68, 260
BciT130I	CCWGG	2 cut(s)	1557, 1875
BciVI	GTATCC	1 cut(s)	1436
BclI	TGATCA	3 cut(s)	330, 523, 1378
BcnI	CCSGG	1 cut(s)	1528
BcoDI	GTCTC	4 cut(s)	76, 1076, 1417, 1616
BfaI	CTAG	4 cut(s)	369, 680, 803, 1608
BfmI	CTRYAG	2 cut(s)	1733, 2133
BfrI	CTTAAG	1 cut(s)	1000
BfuAI	ACCTGC	2 cut(s)	296, 1910
BfuI	GTATCC	1 cut(s)	1436
BmcAI	AGTACT	1 cut(s)	839
Bme1390I	CCNGG	3 cut(s)	1528, 1557, 1875
Bme18I	GGWCC	5 cut(s)	400, 469, 1524, 1553, 1684
BmeT110I	CYCGRG	2 cut(s)	514, 1496
BmgT120I	GGNCC	8 cut(s)	196, 400, 469, 1524, 1553, 1590, 1684, 1981
BmiI	GGNNCC	4 cut(s)	122, 167, 402, 471
BmrFI	CCNGG	3 cut(s)	1528, 1557, 1875
BmrI	ACTGGG	2 cut(s)	850, 2079
BmsI	GCATC	6 cut(s)	865, 910, 1201, 1381, 1587, 1930
BmuI	ACTGGG	2 cut(s)	850, 2079
BpiI	GAAGAC	2 cut(s)	1031, 1893
BplI	GAGNNNNNCTC	4 cut(s)	752, 784, 1479, 1511
Bpu10I	CCTNAGC	1 cut(s)	2061
Bpu14I	TTCGAA	1 cut(s)	1146
BpuEI	CTTGAG	1 cut(s)	1441
BpuMI	CCSGG	1 cut(s)	1528
BsaAI	YACGTR	1 cut(s)	1035
BsaBI	GATNNNNATC	1 cut(s)	416
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	6 cut(s)	230, 382, 572, 1429, 1984, 2099
BsaWI	WCCGGW	1 cut(s)	466
BsaXI	ACNNNNNCTCC	8 cut(s)	44, 59, 74, 89, 1037, 1067, 1841, 1871
Bsc4I	CCNNNNNNNGG	9 cut(s)	22, 232, 236, 369, 397, 726, 1287, 1518, 1764
Bse118I	RCCGGY	2 cut(s)	22, 683
Bse1I	ACTGG	5 cut(s)	625, 845, 1081, 1460, 2085
Bse21I	CCTNAGG	1 cut(s)	1206
Bse8I	GATNNNNATC	1 cut(s)	416
BseBI	CCWGG	2 cut(s)	1557, 1875
BseDI	CCNNGG	6 cut(s)	230, 382, 572, 1429, 1984, 2099
BseGI	GGATG	5 cut(s)	417, 925, 1191, 1381, 1415
BseJI	GATNNNNATC	1 cut(s)	416
BseLI	CCNNNNNNNGG	9 cut(s)	22, 232, 236, 369, 397, 726, 1287, 1518, 1764
BseMII	CTCAG	3 cut(s)	1167, 1197, 2075
BseNI	ACTGG	5 cut(s)	625, 845, 1081, 1460, 2085
BseRI	GAGGAG	8 cut(s)	64, 67, 311, 656, 659, 776, 1193, 1278
BseX3I	CGGCCG	2 cut(s)	24, 379
BseXI	GCAGC	3 cut(s)	640, 2014, 2062
BseYI	CCCAGC	3 cut(s)	362, 474, 1700
Bsh1236I	CGCG	3 cut(s)	384, 1218, 1633
Bsh1285I	CGRYCG	2 cut(s)	27, 382
BshFI	GGCC	8 cut(s)	26, 197, 381, 687, 1592, 1878, 1983, 1989
BsiEI	CGRYCG	2 cut(s)	27, 382
BsiHKAI	GWGCWC	3 cut(s)	1034, 1505, 1648
BsiHKCI	CYCGRG	2 cut(s)	514, 1496
BsiSI	CCGG	5 cut(s)	23, 89, 467, 684, 1527
BslFI	GGGAC	2 cut(s)	455, 1847
BslI	CCNNNNNNNGG	9 cut(s)	22, 232, 236, 369, 397, 726, 1287, 1518, 1764
BsmAI	GTCTC	4 cut(s)	76, 1076, 1417, 1616
BsmBI	CGTCTC	1 cut(s)	76
BsmFI	GGGAC	2 cut(s)	455, 1847
BsmI	GAATGC	2 cut(s)	189, 1972
BsnI	GGCC	8 cut(s)	26, 197, 381, 687, 1592, 1878, 1983, 1989
BsoBI	CYCGRG	2 cut(s)	514, 1496
Bsp119I	TTCGAA	1 cut(s)	1146
Bsp1286I	GDGCHC	3 cut(s)	1034, 1505, 1648
Bsp143I	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
BspANI	GGCC	8 cut(s)	26, 197, 381, 687, 1592, 1878, 1983, 1989
BspCNI	CTCAG	3 cut(s)	1168, 1198, 2074
BspFNI	CGCG	3 cut(s)	384, 1218, 1633
BspLI	GGNNCC	4 cut(s)	122, 167, 402, 471
BspMAI	CTGCAG	1 cut(s)	2137
BspMI	ACCTGC	2 cut(s)	296, 1910
BspPI	GGATC	2 cut(s)	584, 1451
BspT104I	TTCGAA	1 cut(s)	1146
BspTI	CTTAAG	1 cut(s)	1000
BsrBI	CCGCTC	1 cut(s)	106
BsrFI	RCCGGY	2 cut(s)	22, 683
BsrI	ACTGG	5 cut(s)	625, 845, 1081, 1460, 2085
BssAI	RCCGGY	2 cut(s)	22, 683
BssECI	CCNNGG	6 cut(s)	230, 382, 572, 1429, 1984, 2099
BssMI	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	3 cut(s)	230, 1429, 1984
Bst2UI	CCWGG	2 cut(s)	1557, 1875
Bst4CI	ACNGT	3 cut(s)	549, 1639, 1892
Bst6I	CTCTTC	3 cut(s)	206, 317, 677
BstACI	GRCGYC	1 cut(s)	69
BstAFI	CTTAAG	1 cut(s)	1000
BstAPI	GCANNNNNTGC	1 cut(s)	638
BstBAI	YACGTR	1 cut(s)	1035
BstBI	TTCGAA	1 cut(s)	1146
BstC8I	GCNNGC	6 cut(s)	24, 250, 273, 685, 1740, 1972
BstDEI	CTNAG	3 cut(s)	1176, 1206, 2061
BstDSI	CCRYGG	1 cut(s)	382
BstEII	GGTNACC	1 cut(s)	386
BstF5I	GGATG	5 cut(s)	417, 925, 1191, 1381, 1415
BstFNI	CGCG	3 cut(s)	384, 1218, 1633
BstHHI	GCGC	3 cut(s)	160, 1218, 2053
BstKTI	GATC	7 cut(s)	333, 451, 526, 579, 1381, 1459, 1958
BstMAI	GTCTC	4 cut(s)	76, 1076, 1417, 1616
BstMBI	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
BstMCI	CGRYCG	2 cut(s)	27, 382
BstMWI	GCNNNNNNNGC	7 cut(s)	527, 638, 653, 1676, 1722, 1980, 2057
BstNI	CCWGG	2 cut(s)	1557, 1875
BstNSI	RCATGY	3 cut(s)	1114, 1742, 2098
BstPI	GGTNACC	1 cut(s)	386
BstSCI	CCNGG	3 cut(s)	1526, 1555, 1873
BstSFI	CTRYAG	2 cut(s)	1733, 2133
BstUI	CGCG	3 cut(s)	384, 1218, 1633
BstV1I	GCAGC	3 cut(s)	640, 2014, 2062
BstV2I	GAAGAC	2 cut(s)	1031, 1893
BstX2I	RGATCY	1 cut(s)	576
BstXI	CCANNNNNNTGG	2 cut(s)	695, 1997
BstYI	RGATCY	1 cut(s)	576
BstZI	CGGCCG	2 cut(s)	24, 379
Bsu36I	CCTNAGG	1 cut(s)	1206
BsuI	GTATCC	1 cut(s)	1436
BsuRI	GGCC	8 cut(s)	26, 197, 381, 687, 1592, 1878, 1983, 1989
BtgI	CCRYGG	1 cut(s)	382
BtsCI	GGATG	5 cut(s)	417, 925, 1191, 1381, 1415
BtsIMutI	CAGTG	2 cut(s)	545, 1074
BveI	ACCTGC	2 cut(s)	296, 1910
Cac8I	GCNNGC	6 cut(s)	24, 250, 273, 685, 1740, 1972
CaiI	CAGNNNCTG	1 cut(s)	1021
CfoI	GCGC	3 cut(s)	160, 1218, 2053
Cfr10I	RCCGGY	2 cut(s)	22, 683
Cfr13I	GGNCC	8 cut(s)	196, 400, 469, 1524, 1553, 1590, 1684, 1981
Cfr42I	CCGCGG	1 cut(s)	385
CsiI	ACCWGGT	1 cut(s)	1555
Csp6I	GTAC	9 cut(s)	565, 658, 816, 838, 967, 1036, 1243, 1750, 1858
CviAII	CATG	9 cut(s)	328, 527, 642, 1111, 1313, 1587, 1739, 1773, 2095
CviQI	GTAC	9 cut(s)	565, 658, 816, 838, 967, 1036, 1243, 1750, 1858
DdeI	CTNAG	3 cut(s)	1176, 1206, 2061
DpnI	GATC	7 cut(s)	332, 450, 525, 578, 1380, 1458, 1957
DpnII	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
DrdI	GACNNNNNNGTC	1 cut(s)	443
DseDI	GACNNNNNNGTC	1 cut(s)	443
EaeI	YGGCCR	3 cut(s)	24, 379, 685
EagI	CGGCCG	2 cut(s)	24, 379
Eam1104I	CTCTTC	3 cut(s)	206, 317, 677
EarI	CTCTTC	3 cut(s)	206, 317, 677
EciI	GGCGGA	3 cut(s)	26, 52, 70
Ecl136II	GAGCTC	2 cut(s)	1503, 1646
EclXI	CGGCCG	2 cut(s)	24, 379
Eco130I	CCWWGG	3 cut(s)	230, 1429, 1984
Eco24I	GRGCYC	2 cut(s)	1505, 1648
Eco32I	GATATC	1 cut(s)	151
Eco47I	GGWCC	5 cut(s)	400, 469, 1524, 1553, 1684
Eco52I	CGGCCG	2 cut(s)	24, 379
Eco53kI	GAGCTC	2 cut(s)	1503, 1646
Eco57I	CTGAAG	1 cut(s)	1043
Eco81I	CCTNAGG	1 cut(s)	1206
Eco88I	CYCGRG	2 cut(s)	514, 1496
Eco91I	GGTNACC	1 cut(s)	386
EcoICRI	GAGCTC	2 cut(s)	1503, 1646
EcoO109I	RGGNCCY	1 cut(s)	1684
EcoO65I	GGTNACC	1 cut(s)	386
EcoRII	CCWGG	2 cut(s)	1555, 1873
EcoRV	GATATC	1 cut(s)	151
EcoT14I	CCWWGG	3 cut(s)	230, 1429, 1984
EcoT38I	GRGCYC	2 cut(s)	1505, 1648
ErhI	CCWWGG	3 cut(s)	230, 1429, 1984
Esp3I	CGTCTC	1 cut(s)	76
FaeI	CATG	9 cut(s)	331, 530, 645, 1114, 1316, 1590, 1742, 1776, 2098
FalI	AAGNNNNNCTT	2 cut(s)	422, 454
FaqI	GGGAC	2 cut(s)	455, 1847
FatI	CATG	9 cut(s)	327, 526, 641, 1110, 1312, 1586, 1738, 1772, 2094
FauI	CCCGC	2 cut(s)	111, 640
FauNDI	CATATG	2 cut(s)	1009, 1397
FbaI	TGATCA	3 cut(s)	330, 523, 1378
FblI	GTMKAC	1 cut(s)	207
FokI	GGATG	5 cut(s)	404, 932, 1198, 1388, 1402
FriOI	GRGCYC	2 cut(s)	1505, 1648
FspBI	CTAG	4 cut(s)	369, 680, 803, 1608
FspI	TGCGCA	1 cut(s)	2052
GlaI	GCGC	3 cut(s)	159, 1217, 2052
GsaI	CCCAGC	3 cut(s)	366, 478, 1704
HaeIII	GGCC	8 cut(s)	26, 197, 381, 687, 1592, 1878, 1983, 1989
HapII	CCGG	5 cut(s)	23, 89, 467, 684, 1527
HhaI	GCGC	3 cut(s)	160, 1218, 2053
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	9 cut(s)	331, 530, 645, 1114, 1316, 1590, 1742, 1776, 2098
Hin6I	GCGC	3 cut(s)	158, 1216, 2051
HinP1I	GCGC	3 cut(s)	158, 1216, 2051
HincII	GTYRAC	2 cut(s)	208, 847
HindII	GTYRAC	2 cut(s)	208, 847
HindIII	AAGCTT	1 cut(s)	1662
HinfI	GANTC	5 cut(s)	496, 559, 598, 1018, 1277
HpaII	CCGG	5 cut(s)	23, 89, 467, 684, 1527
HphI	GGTGA	9 cut(s)	277, 295, 398, 505, 844, 1466, 1571, 1949, 1961
Hpy166II	GTNNAC	2 cut(s)	208, 847
Hpy188I	TCNGA	4 cut(s)	436, 576, 1023, 1643
Hpy188III	TCNNGA	7 cut(s)	516, 803, 928, 1388, 1448, 1498, 1959
Hpy8I	GTNNAC	2 cut(s)	208, 847
Hpy99I	CGWCG	5 cut(s)	71, 209, 281, 284, 1353
HpyAV	CCTTC	4 cut(s)	473, 1632, 1727, 1800
HpyCH4III	ACNGT	3 cut(s)	549, 1639, 1892
HpyCH4IV	ACGT	6 cut(s)	69, 604, 849, 1034, 1222, 1405
HpyF10VI	GCNNNNNNNGC	7 cut(s)	527, 638, 653, 1676, 1722, 1980, 2057
HpyF3I	CTNAG	3 cut(s)	1176, 1206, 2061
HpySE526I	ACGT	6 cut(s)	69, 604, 849, 1034, 1222, 1405
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	9 cut(s)	331, 530, 645, 1114, 1316, 1590, 1742, 1776, 2098
HspAI	GCGC	3 cut(s)	158, 1216, 2051
KroI	GCCGGC	2 cut(s)	22, 683
KroNI	GCCGGC	2 cut(s)	24, 685
Ksp22I	TGATCA	3 cut(s)	330, 523, 1378
KspI	CCGCGG	1 cut(s)	385
Kzo9I	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
LmnI	GCTCC	2 cut(s)	650, 1508
Lsp1109I	GCAGC	3 cut(s)	640, 2014, 2062
LweI	GCATC	6 cut(s)	865, 910, 1201, 1381, 1587, 1930
MabI	ACCWGGT	1 cut(s)	1555
MaeI	CTAG	4 cut(s)	369, 680, 803, 1608
MaeII	ACGT	6 cut(s)	69, 604, 849, 1034, 1222, 1405
MaeIII	GTNAC	8 cut(s)	283, 386, 394, 850, 1200, 1559, 1892, 2002
MalI	GATC	7 cut(s)	332, 450, 525, 578, 1380, 1458, 1957
MbiI	CCGCTC	1 cut(s)	106
MboI	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
MflI	RGATCY	1 cut(s)	576
MhlI	GDGCHC	3 cut(s)	1034, 1505, 1648
MlyI	GAGTC	1 cut(s)	1012
MmeI	TCCRAC	1 cut(s)	459
MroNI	GCCGGC	2 cut(s)	22, 683
MroXI	GAANNNNTTC	1 cut(s)	1224
MseI	TTAA	9 cut(s)	864, 900, 992, 1001, 1161, 1536, 1689, 1812, 2108
MspA1I	CMGCKG	2 cut(s)	384, 1324
MspCI	CTTAAG	1 cut(s)	1000
MspI	CCGG	5 cut(s)	23, 89, 467, 684, 1527
MspR9I	CCNGG	3 cut(s)	1528, 1557, 1875
Mva1269I	GAATGC	2 cut(s)	189, 1972
MvaI	CCWGG	2 cut(s)	1557, 1875
MvnI	CGCG	3 cut(s)	384, 1218, 1633
MwoI	GCNNNNNNNGC	7 cut(s)	527, 638, 653, 1676, 1722, 1980, 2057
NaeI	GCCGGC	2 cut(s)	24, 685
NciI	CCSGG	1 cut(s)	1528
NdeI	CATATG	2 cut(s)	1009, 1397
NdeII	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
NgoMIV	GCCGGC	2 cut(s)	22, 683
NlaIII	CATG	9 cut(s)	331, 530, 645, 1114, 1316, 1590, 1742, 1776, 2098
NlaIV	GGNNCC	4 cut(s)	122, 167, 402, 471
NmuCI	GTSAC	6 cut(s)	283, 386, 394, 850, 1559, 2002
NsbI	TGCGCA	1 cut(s)	2052
NspI	RCATGY	3 cut(s)	1114, 1742, 2098
NspV	TTCGAA	1 cut(s)	1146
PaeI	GCATGC	1 cut(s)	1742
PaeR7I	CTCGAG	2 cut(s)	514, 1496
PaqCI	CACCTGC	2 cut(s)	296, 1910
PcsI	WCGNNNNNNNCGW	2 cut(s)	279, 512
PctI	GAATGC	2 cut(s)	189, 1972
PdiI	GCCGGC	2 cut(s)	24, 685
PdmI	GAANNNNTTC	1 cut(s)	1224
PfeI	GAWTC	4 cut(s)	496, 559, 598, 1277
PflFI	GACNNNGTC	1 cut(s)	392
PleI	GAGTC	1 cut(s)	1012
PpsI	GAGTC	1 cut(s)	1012
Ppu21I	YACGTR	1 cut(s)	1035
PpuMI	RGGWCCY	1 cut(s)	1684
Psp124BI	GAGCTC	2 cut(s)	1505, 1648
Psp5II	RGGWCCY	1 cut(s)	1684
Psp6I	CCWGG	2 cut(s)	1555, 1873
PspEI	GGTNACC	1 cut(s)	386
PspFI	CCCAGC	3 cut(s)	362, 474, 1700
PspGI	CCWGG	2 cut(s)	1555, 1873
PspN4I	GGNNCC	4 cut(s)	122, 167, 402, 471
PspPI	GGNCC	8 cut(s)	196, 400, 469, 1524, 1553, 1590, 1684, 1981
PspPPI	RGGWCCY	1 cut(s)	1684
PstI	CTGCAG	1 cut(s)	2137
PstNI	CAGNNNCTG	1 cut(s)	1021
PsuI	RGATCY	1 cut(s)	576
PsyI	GACNNNGTC	1 cut(s)	392
RsaI	GTAC	9 cut(s)	566, 659, 817, 839, 968, 1037, 1244, 1751, 1859
RsaNI	GTAC	9 cut(s)	565, 658, 816, 838, 967, 1036, 1243, 1750, 1858
SacI	GAGCTC	2 cut(s)	1505, 1648
SacII	CCGCGG	1 cut(s)	385
SalI	GTCGAC	1 cut(s)	206
SaqAI	TTAA	9 cut(s)	864, 900, 992, 1001, 1161, 1536, 1689, 1812, 2108
Sau3AI	GATC	7 cut(s)	330, 448, 523, 576, 1378, 1456, 1955
Sau96I	GGNCC	8 cut(s)	196, 400, 469, 1524, 1553, 1590, 1684, 1981
ScaI	AGTACT	1 cut(s)	839
SchI	GAGTC	1 cut(s)	1012
ScrFI	CCNGG	3 cut(s)	1528, 1557, 1875
SduI	GDGCHC	3 cut(s)	1034, 1505, 1648
SexAI	ACCWGGT	1 cut(s)	1555
SfaNI	GCATC	6 cut(s)	865, 910, 1201, 1381, 1587, 1930
SfcI	CTRYAG	2 cut(s)	1733, 2133
Sfr274I	CTCGAG	2 cut(s)	514, 1496
Sfr303I	CCGCGG	1 cut(s)	385
SfuI	TTCGAA	1 cut(s)	1146
SgrBI	CCGCGG	1 cut(s)	385
SinI	GGWCC	5 cut(s)	400, 469, 1524, 1553, 1684
SlaI	CTCGAG	2 cut(s)	514, 1496
SmlI	CTYRAG	4 cut(s)	514, 1000, 1420, 1496
SmoI	CTYRAG	4 cut(s)	514, 1000, 1420, 1496
SphI	GCATGC	1 cut(s)	1742
SspMI	CTAG	4 cut(s)	369, 680, 803, 1608
SstI	GAGCTC	2 cut(s)	1505, 1648
StyD4I	CCNGG	3 cut(s)	1526, 1555, 1873
StyI	CCWWGG	3 cut(s)	230, 1429, 1984
TaaI	ACNGT	3 cut(s)	549, 1639, 1892
TaiI	ACGT	6 cut(s)	72, 607, 852, 1037, 1225, 1408
TatI	WGTACW	5 cut(s)	564, 815, 837, 1242, 1857
TauI	GCSGC	7 cut(s)	29, 384, 458, 730, 1327, 1572, 1799
TfiI	GAWTC	4 cut(s)	496, 559, 598, 1277
Tru1I	TTAA	9 cut(s)	864, 900, 992, 1001, 1161, 1536, 1689, 1812, 2108
Tru9I	TTAA	9 cut(s)	864, 900, 992, 1001, 1161, 1536, 1689, 1812, 2108
TscAI	CASTG	2 cut(s)	552, 1081
TseFI	GTSAC	6 cut(s)	283, 386, 394, 850, 1559, 2002
TseI	GCWGC	3 cut(s)	653, 2027, 2075
Tsp45I	GTSAC	6 cut(s)	283, 386, 394, 850, 1559, 2002
TspDTI	ATGAA	6 cut(s)	17, 430, 996, 1287, 1329, 2133
TspGWI	ACGGA	2 cut(s)	413, 847
TspRI	CASTG	2 cut(s)	552, 1081
Tth111I	GACNNNGTC	1 cut(s)	392
Vha464I	CTTAAG	1 cut(s)	1000
VpaK11BI	GGWCC	5 cut(s)	400, 469, 1524, 1553, 1684
XapI	RAATTY	5 cut(s)	32, 896, 909, 1142, 1691
XbaI	TCTAGA	1 cut(s)	802
XceI	RCATGY	3 cut(s)	1114, 1742, 2098
XcmI	CCANNNNNNNNNTGG	1 cut(s)	538
XhoI	CTCGAG	2 cut(s)	514, 1496
XmiI	GTMKAC	1 cut(s)	207
XmnI	GAANNNNTTC	1 cut(s)	1224
XspI	CTAG	4 cut(s)	369, 680, 803, 1608
ZraI	GACGTC	1 cut(s)	70
ZrmI	AGTACT	1 cut(s)	839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.