Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0016495

Physical Location & Seq

Genomic Coordinates Forward (+)

87413827 .. 87418090

Gene Structure 4264 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG647600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGAGTAGACCAATCACCACGCCGGTCAAATTCCGCCCTCTCCAGAACCCTAGCGGCGACGGCGCCACCTCGGCCGCCGTAGTAGACGAGCACTCGCTCCACATTCTATGGCGACGTCACAAGATTCTCGACCCCGACTCCGAACTCGTATCCCTCTGGCACCACATCTTCCTCGTCATCTGCATCGCCGCGCTCTTCATCGACCCTCTCTACTTCTACCTCCCCTTCGTCGGCGGCGAGGGTTGCCTCTCCACCGACCACCGACTCGCCGTCATCGTCACCTTCTTCCGCTCCATCGCCGACCTCTTCTACCTCTTCCACATGGTCGTCAAGTTCCGCACCGCATTCATCGCCCCCAGCTCTAGGGTTTTCGGCCGCGGAGATCTCGTCATGGACGGCAGGCAGATCGCCATGCGGTATCTCAAGTCCGACTTCGTGGTCGATCTGGCTGCCACTCTTCCAATTCCGCAGATTGTTATCTGGCTGGTGATTCCAGCAACGAACAATGCGGGCATTTATCATGCCAACAACACTCTGGCCCTTTTCGTGCTTATTCAATATATTCCTAGAATATTTCTCATATTTCCTTTGAATCAGAAAATTGTGAAGACTACTGGGGTTGTAGCCAAGACTCCCTGGGCAGGAGCTGCGTACAATTTGCTTCTTTTCATGCTAGCCAGTCATATATTTGGAGCCGTTTGGTATCTTTGTGCCATAGGGCGGCAATTTGACTGTTGGAAAACGCAGTGTAGTGAAGAGAATACAAAAGTTGTGACTTGCCTCTATAGTTTCCTGGATTGCAAGAGTCTGGACCTACCAGAACGGGAATCTTGGGCCAACGCCACCGTAGTAATAAAAAAGTGTGATGCCTCTAATAAAAAGGCCGATTTTAAGTTTGGAATCTTTAAGAATGCGTTTAAGACTGGGGCTGCAAAGACGAACTTTTTTGAGAAGTACTTGTTTTGTTTCTGGTGGGGTTTAAGAAACTTATGCTCATACGGGCAGACTTTGATGACGAGCACATATATTGGCGAGACATTGTTCTCCATCGTCTTGTGCATTTCTGGTTTGATTTTGTTCTCACTATTGATCGGCAACATGCAGACTTATCTGCAATCAATGAAAATAAAAATCGAAGAATGGAGGATTAAGCAAAGAGATACTGATGAGTGGATGAGGCATCGCCAGTTACCTGAACCCTTGCAAGAACGTGTTCGTAGATTTATGCAATACAAGTGGTTTGCAACACGAGGAGTTGATGAAGAATCCATATTGCATTCATTACCCATCGACCTCTGCCATGAAATCCAGCAGCACTTATGCCTTTCCCTTGTTCGTCGTGTCCCTTTTTTCTCACAAATGGATGACCAGCTTCTTGATGCCATATGTGAGCGTCTTGTGTCATCCTTGACTATCAAAGGCACATATATAGTTCAAGAAGGTGACCCAGTAAATGAGATGCTGTTTATTATTAGAGGGGAACTTGAGAGCTCCACAACCAATGGAGGGAGGACTGGCTTCTTTAACTCCATCAATCTAGGACCTGGTGACTTCTGTGGTGAGGAACTATTGACTTGGGCATTGATGCCAAGTTCTAGCGTTAGCATGCCTTCTTCCACTCGGACTGTGAGAGCCCTAACTGAAGTTGAAGCTTTTGCGCTTCGAGCTGAGGACCTTAAGTTTGTTGCAAGTCAGTTCAAACGCCTTCACAGCAAGAAACTACAGCATGCCTTTAGGTACTATTCTCACCAATGGAGGACATGGGGTGCTTGCTTCATACAAGCTGCTTGGAGAAGGTTTAAGAAGAGGAAGATGGCAAGGGACTTGGCTATGCAGGAGGGCTTATACTATATGCATGTACCTGGCCAAGAGGAAGAGGGTTACTACTACGACAACGAAGAACAAACAGATCTTGGAAACTATGATGGTGAGAGTGGTGATAGGTCATCGACTGGGGAGAGGCTAAGCACTTCCATGAATCTTGGGGCCACATTTCTGGCTTCTAAATTTGCTGCAAATACAAGAAGAGGGATTGTGCAGAAAGCTCAGCTGGTTGATCCTGATTCAACTAGTTTGCAGATGCCTCGGATGTTTAAGCCAGATGAGCCTGATTTTTCTGTAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0008324 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016324 GO:0019725 GO:0022414 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0034220 GO:0040007 GO:0042391 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0046873 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051704 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072511 GO:0098590 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

708

Amino Acids

81.02

Weight (kDa)

8.34

Isoelectric Point (pI)

40.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	52 - 375	4.3e-21	Ion transport protein
cNMP_binding	PF00027	473 - 562	1.6e-09	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14870
fragaria_vesca	FvH4_6g50510
malus_domestica	MD09G1035800.v1.1 MD17G1037400.v1.1
prunus_persica	Prupe.3G282200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0170931
rosa_laevigata	RLG00000021993
rosa_multiflora	Rmu_sc0025148.1_g000002
rosa_roxburghii	Rroxscaffold_2G00080970
rosa_rugosa	Rorug02G0552400
rosa_samantha	Rh2BG636000 Rh2CG604500 Rh2DG647600
rosa_wichuraiana	Rw2G051780

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	437
AatII	GACGTC	1 cut(s)	118
AccB1I	GGYRCC	2 cut(s)	62, 159
AccBSI	CCGCTC	1 cut(s)	291
AccI	GTMKAC	2 cut(s)	7, 84
AccII	CGCG	2 cut(s)	191, 378
AclWI	GGATC	1 cut(s)	2051
AcoI	YGGCCR	3 cut(s)	72, 373, 1864
AcsI	RAATTY	2 cut(s)	29, 2006
AcuI	CTGAAG	1 cut(s)	1662
AcyI	GRCGYC	2 cut(s)	63, 115
AfaI	GTAC	4 cut(s)	653, 956, 1739, 1860
AfiI	CCNNNNNNNGG	5 cut(s)	230, 363, 641, 720, 1506
AflII	CTTAAG	1 cut(s)	1676
AflIII	ACRYGT	1 cut(s)	1210
AgsI	TTSAA	6 cut(s)	557, 592, 1436, 1649, 1699, 2067
AhdI	GACNNNNNGTC	1 cut(s)	269
AhlI	ACTAGT	1 cut(s)	2069
AjnI	CCWGG	4 cut(s)	635, 792, 1543, 1861
AluBI	AGCT	9 cut(s)	360, 647, 1372, 1491, 1652, 1667, 1784, 2045, 2050
AluI	AGCT	9 cut(s)	360, 647, 1372, 1491, 1652, 1667, 1784, 2045, 2050
Alw21I	GWGCWC	3 cut(s)	93, 1022, 1493
Alw26I	GTCTC	1 cut(s)	1028
AlwI	GGATC	1 cut(s)	2051
AlwNI	CAGNNNCTG	1 cut(s)	647
AoxI	GGCC	7 cut(s)	72, 373, 537, 834, 882, 1864, 1986
ApeKI	GCWGC	6 cut(s)	449, 647, 929, 1312, 1784, 2012
ApoI	RAATTY	2 cut(s)	29, 2006
ArsI	GACNNNNNNTTYG	2 cut(s)	1664, 1696
Asp700I	GAANNNNTTC	1 cut(s)	1212
AspLEI	GCGC	3 cut(s)	65, 193, 1660
AspS9I	GGNCC	6 cut(s)	538, 811, 834, 1541, 1672, 1986
AsuHPI	GGTGA	9 cut(s)	7, 271, 499, 1454, 1559, 1571, 1739, 1940, 1949
AsuNHI	GCTAGC	1 cut(s)	673
AvaII	GGWCC	3 cut(s)	811, 1541, 1672
BalI	TGGCCA	1 cut(s)	1866
BanI	GGYRCC	2 cut(s)	62, 159
BanII	GRGCYC	2 cut(s)	1493, 1636
BauI	CACGAG	1 cut(s)	1248
BbsI	GAAGAC	1 cut(s)	614
Bbv12I	GWGCWC	3 cut(s)	93, 1022, 1493
BbvCI	CCTCAGC	1 cut(s)	1668
BbvI	GCAGC	6 cut(s)	436, 634, 916, 1324, 1771, 1999
BccI	CCATC	6 cut(s)	302, 1055, 1295, 1538, 1807, 1919
BceAI	ACGGC	5 cut(s)	62, 76, 254, 412, 680
BcgI	CGANNNNNNTGC	6 cut(s)	388, 422, 431, 465, 2066, 2100
BciT130I	CCWGG	4 cut(s)	637, 794, 1545, 1863
BciVI	GTATCC	1 cut(s)	160
BcoDI	GTCTC	1 cut(s)	1028
BcuI	ACTAGT	1 cut(s)	2069
BfaI	CTAG	7 cut(s)	51, 363, 567, 674, 1538, 1596, 2070
BfmI	CTRYAG	3 cut(s)	784, 1721, 2118
BfoI	RGCGCY	1 cut(s)	66
BfrI	CTTAAG	1 cut(s)	1676
BfuI	GTATCC	1 cut(s)	160
BglI	GCCNNNNNGGC	1 cut(s)	71
BglII	AGATCT	2 cut(s)	382, 1909
BlpI	GCTNAGC	2 cut(s)	1964, 2046
BmcAI	AGTACT	1 cut(s)	956
Bme1390I	CCNGG	4 cut(s)	637, 794, 1545, 1863
Bme18I	GGWCC	3 cut(s)	811, 1541, 1672
BmeRI	GACNNNNNGTC	1 cut(s)	269
BmgT120I	GGNCC	6 cut(s)	538, 811, 834, 1541, 1672, 1986
BmiI	GGNNCC	4 cut(s)	64, 161, 694, 1987
BmrFI	CCNGG	4 cut(s)	637, 794, 1545, 1863
BmrI	ACTGGG	4 cut(s)	624, 933, 1442, 1962
BmsI	GCATC	7 cut(s)	192, 856, 1189, 1369, 1449, 1575, 2070
BmtI	GCTAGC	1 cut(s)	677
BmuI	ACTGGG	4 cut(s)	624, 933, 1442, 1962
BpiI	GAAGAC	1 cut(s)	614
BpmI	CTGGAG	1 cut(s)	26
Bpu10I	CCTNAGC	1 cut(s)	1668
Bpu1102I	GCTNAGC	2 cut(s)	1964, 2046
BpuEI	CTTGAG	2 cut(s)	407, 1505
BsaBI	GATNNNNATC	1 cut(s)	476
BsaHI	GRCGYC	2 cut(s)	63, 115
BsaJI	CCNNGG	5 cut(s)	69, 376, 635, 636, 2084
BsaXI	ACNNNNNCTCC	4 cut(s)	122, 152, 372, 402
Bsc4I	CCNNNNNNNGG	5 cut(s)	230, 363, 641, 720, 1506
Bse118I	RCCGGY	1 cut(s)	22
Bse1I	ACTGG	7 cut(s)	619, 678, 928, 1186, 1448, 1519, 1957
Bse8I	GATNNNNATC	1 cut(s)	476
BseBI	CCWGG	4 cut(s)	637, 794, 1545, 1863
BseDI	CCNNGG	5 cut(s)	69, 376, 635, 636, 2084
BseGI	GGATG	4 cut(s)	1179, 1369, 1403, 2094
BseJI	GATNNNNATC	1 cut(s)	476
BseLI	CCNNNNNNNGG	5 cut(s)	230, 363, 641, 720, 1506
BseMII	CTCAG	2 cut(s)	1659, 2060
BseNI	ACTGG	7 cut(s)	619, 678, 928, 1186, 1448, 1519, 1957
BseRI	GAGGAG	1 cut(s)	1266
BseX3I	CGGCCG	2 cut(s)	72, 373
BseXI	GCAGC	6 cut(s)	436, 634, 916, 1324, 1771, 1999
BseYI	CCCAGC	1 cut(s)	356
BsgI	GTGCAG	1 cut(s)	2057
Bsh1236I	CGCG	2 cut(s)	191, 378
Bsh1285I	CGRYCG	2 cut(s)	75, 376
BshFI	GGCC	7 cut(s)	74, 375, 539, 836, 884, 1866, 1988
BshNI	GGYRCC	2 cut(s)	62, 159
BsiEI	CGRYCG	2 cut(s)	75, 376
BsiHKAI	GWGCWC	3 cut(s)	93, 1022, 1493
BsiSI	CCGG	1 cut(s)	23
BslFI	GGGAC	2 cut(s)	1328, 1835
BslI	CCNNNNNNNGG	5 cut(s)	230, 363, 641, 720, 1506
BsmAI	GTCTC	1 cut(s)	1028
BsmFI	GGGAC	2 cut(s)	1328, 1835
BsmI	GAATGC	3 cut(s)	344, 916, 1276
BsnI	GGCC	7 cut(s)	74, 375, 539, 836, 884, 1866, 1988
Bsp1286I	GDGCHC	4 cut(s)	93, 1022, 1493, 1636
Bsp143I	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
Bsp1720I	GCTNAGC	2 cut(s)	1964, 2046
BspANI	GGCC	7 cut(s)	74, 375, 539, 836, 884, 1866, 1988
BspCNI	CTCAG	2 cut(s)	1660, 2059
BspFNI	CGCG	2 cut(s)	191, 378
BspLI	GGNNCC	4 cut(s)	64, 161, 694, 1987
BspOI	GCTAGC	1 cut(s)	677
BspPI	GGATC	1 cut(s)	2051
BspQI	GCTCTTC	1 cut(s)	200
BspT107I	GGYRCC	2 cut(s)	62, 159
BspTI	CTTAAG	1 cut(s)	1676
BsrBI	CCGCTC	1 cut(s)	291
BsrFI	RCCGGY	1 cut(s)	22
BsrI	ACTGG	7 cut(s)	619, 678, 928, 1186, 1448, 1519, 1957
BssAI	RCCGGY	1 cut(s)	22
BssECI	CCNNGG	5 cut(s)	69, 376, 635, 636, 2084
BssMI	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
BssNI	GRCGYC	2 cut(s)	63, 115
BssSI	CACGAG	1 cut(s)	1248
Bst2BI	CACGAG	1 cut(s)	1248
Bst2UI	CCWGG	4 cut(s)	637, 794, 1545, 1863
Bst4CI	ACNGT	3 cut(s)	734, 847, 1627
Bst6I	CTCTTC	8 cut(s)	200, 311, 320, 462, 750, 1799, 1869, 2020
BstACI	GRCGYC	2 cut(s)	63, 115
BstAFI	CTTAAG	1 cut(s)	1676
BstAPI	GCANNNNNTGC	1 cut(s)	647
BstC8I	GCNNGC	6 cut(s)	401, 511, 675, 1607, 1728, 1771
BstDEI	CTNAG	3 cut(s)	1668, 1964, 2046
BstDSI	CCRYGG	1 cut(s)	376
BstEII	GGTNACC	1 cut(s)	1442
BstF5I	GGATG	4 cut(s)	1179, 1369, 1403, 2094
BstFNI	CGCG	2 cut(s)	191, 378
BstH2I	RGCGCY	1 cut(s)	66
BstHHI	GCGC	3 cut(s)	65, 193, 1660
BstKTI	GATC	6 cut(s)	385, 408, 445, 1092, 1912, 2059
BstMAI	GTCTC	1 cut(s)	1028
BstMBI	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
BstMCI	CGRYCG	2 cut(s)	75, 376
BstMWI	GCNNNNNNNGC	8 cut(s)	60, 71, 243, 350, 647, 1664, 1710, 2104
BstNI	CCWGG	4 cut(s)	637, 794, 1545, 1863
BstNSI	RCATGY	4 cut(s)	1102, 1609, 1730, 1859
BstPI	GGTNACC	1 cut(s)	1442
BstSCI	CCNGG	4 cut(s)	635, 792, 1543, 1861
BstSFI	CTRYAG	3 cut(s)	784, 1721, 2118
BstUI	CGCG	2 cut(s)	191, 378
BstV1I	GCAGC	6 cut(s)	436, 634, 916, 1324, 1771, 1999
BstV2I	GAAGAC	1 cut(s)	614
BstX2I	RGATCY	2 cut(s)	382, 1909
BstXI	CCANNNNNNTGG	1 cut(s)	1996
BstYI	RGATCY	2 cut(s)	382, 1909
BstZI	CGGCCG	2 cut(s)	72, 373
BsuI	GTATCC	1 cut(s)	160
BsuRI	GGCC	7 cut(s)	74, 375, 539, 836, 884, 1866, 1988
BtgI	CCRYGG	1 cut(s)	376
BtgZI	GCGATG	4 cut(s)	169, 280, 334, 1166
BtsCI	GGATG	4 cut(s)	1179, 1369, 1403, 2094
BtsI	GCAGTG	1 cut(s)	752
BtsIMutI	CAGTG	1 cut(s)	752
Cac8I	GCNNGC	6 cut(s)	401, 511, 675, 1607, 1728, 1771
CaiI	CAGNNNCTG	1 cut(s)	647
CfoI	GCGC	3 cut(s)	65, 193, 1660
Cfr10I	RCCGGY	1 cut(s)	22
Cfr13I	GGNCC	6 cut(s)	538, 811, 834, 1541, 1672, 1986
Cfr42I	CCGCGG	1 cut(s)	379
CseI	GACGC	1 cut(s)	1382
CsiI	ACCWGGT	1 cut(s)	1543
Csp6I	GTAC	4 cut(s)	652, 955, 1738, 1859
CspCI	CAANNNNNGTGG	2 cut(s)	1217, 1252
CviQI	GTAC	4 cut(s)	652, 955, 1738, 1859
DdeI	CTNAG	3 cut(s)	1668, 1964, 2046
DinI	GGCGCC	1 cut(s)	64
DpnI	GATC	6 cut(s)	384, 407, 444, 1091, 1911, 2058
DpnII	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
DrdI	GACNNNNNNGTC	1 cut(s)	437
DriI	GACNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	437
EaeI	YGGCCR	3 cut(s)	72, 373, 1864
EagI	CGGCCG	2 cut(s)	72, 373
Eam1104I	CTCTTC	8 cut(s)	200, 311, 320, 462, 750, 1799, 1869, 2020
Eam1105I	GACNNNNNGTC	1 cut(s)	269
EarI	CTCTTC	8 cut(s)	200, 311, 320, 462, 750, 1799, 1869, 2020
EciI	GGCGGA	1 cut(s)	23
Ecl136II	GAGCTC	1 cut(s)	1491
EclXI	CGGCCG	2 cut(s)	72, 373
Eco24I	GRGCYC	2 cut(s)	1493, 1636
Eco47I	GGWCC	3 cut(s)	811, 1541, 1672
Eco52I	CGGCCG	2 cut(s)	72, 373
Eco53kI	GAGCTC	1 cut(s)	1491
Eco57I	CTGAAG	1 cut(s)	1662
Eco91I	GGTNACC	1 cut(s)	1442
EcoICRI	GAGCTC	1 cut(s)	1491
EcoO109I	RGGNCCY	2 cut(s)	1541, 1672
EcoO65I	GGTNACC	1 cut(s)	1442
EcoRII	CCWGG	4 cut(s)	635, 792, 1543, 1861
EcoT22I	ATGCAT	1 cut(s)	1857
EcoT38I	GRGCYC	2 cut(s)	1493, 1636
EgeI	GGCGCC	1 cut(s)	64
EheI	GGCGCC	1 cut(s)	64
FalI	AAGNNNNNCTT	4 cut(s)	416, 448, 926, 958
FaqI	GGGAC	2 cut(s)	1328, 1835
FauI	CCCGC	1 cut(s)	502
FauNDI	CATATG	1 cut(s)	1385
FblI	GTMKAC	2 cut(s)	7, 84
FokI	GGATG	4 cut(s)	1186, 1376, 1390, 2101
FriOI	GRGCYC	2 cut(s)	1493, 1636
FspBI	CTAG	7 cut(s)	51, 363, 567, 674, 1538, 1596, 2070
GlaI	GCGC	3 cut(s)	64, 192, 1659
GsaI	CCCAGC	1 cut(s)	360
GsuI	CTGGAG	1 cut(s)	26
HaeII	RGCGCY	1 cut(s)	66
HaeIII	GGCC	7 cut(s)	74, 375, 539, 836, 884, 1866, 1988
HapII	CCGG	1 cut(s)	23
HgaI	GACGC	1 cut(s)	1382
HhaI	GCGC	3 cut(s)	65, 193, 1660
Hin1I	GRCGYC	2 cut(s)	63, 115
Hin6I	GCGC	3 cut(s)	63, 191, 1658
HinP1I	GCGC	3 cut(s)	63, 191, 1658
HindIII	AAGCTT	1 cut(s)	1650
HpaII	CCGG	1 cut(s)	23
HphI	GGTGA	9 cut(s)	7, 271, 499, 1454, 1559, 1571, 1739, 1940, 1949
Hpy166II	GTNNAC	2 cut(s)	8, 85
Hpy188I	TCNGA	5 cut(s)	142, 430, 597, 1623, 2088
Hpy188III	TCNNGA	6 cut(s)	43, 128, 809, 1376, 1436, 2060
Hpy8I	GTNNAC	2 cut(s)	8, 85
Hpy99I	CGWCG	4 cut(s)	62, 117, 233, 1341
HpyAV	CCTTC	6 cut(s)	235, 292, 1433, 1620, 1715, 1788
HpyCH4III	ACNGT	3 cut(s)	734, 847, 1627
HpyCH4IV	ACGT	2 cut(s)	115, 1210
HpyF10VI	GCNNNNNNNGC	8 cut(s)	60, 71, 243, 350, 647, 1664, 1710, 2104
HpyF3I	CTNAG	3 cut(s)	1668, 1964, 2046
HpySE526I	ACGT	2 cut(s)	115, 1210
Hsp92I	GRCGYC	2 cut(s)	63, 115
HspAI	GCGC	3 cut(s)	63, 191, 1658
KasI	GGCGCC	1 cut(s)	62
KspI	CCGCGG	1 cut(s)	379
Kzo9I	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
LguI	GCTCTTC	1 cut(s)	200
LmnI	GCTCC	5 cut(s)	102, 296, 644, 692, 1496
Lsp1109I	GCAGC	6 cut(s)	436, 634, 916, 1324, 1771, 1999
LweI	GCATC	7 cut(s)	192, 856, 1189, 1369, 1449, 1575, 2070
MabI	ACCWGGT	1 cut(s)	1543
MaeI	CTAG	7 cut(s)	51, 363, 567, 674, 1538, 1596, 2070
MaeII	ACGT	2 cut(s)	115, 1210
MaeIII	GTNAC	7 cut(s)	116, 277, 772, 1188, 1442, 1547, 1880
MalI	GATC	6 cut(s)	384, 407, 444, 1091, 1911, 2058
MbiI	CCGCTC	1 cut(s)	291
MboI	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
MflI	RGATCY	2 cut(s)	382, 1909
MhlI	GDGCHC	4 cut(s)	93, 1022, 1493, 1636
MlsI	TGGCCA	1 cut(s)	1866
MluCI	AATT	6 cut(s)	29, 462, 600, 655, 725, 2006
MluNI	TGGCCA	1 cut(s)	1866
Mly113I	GGCGCC	1 cut(s)	63
MlyI	GAGTC	4 cut(s)	131, 258, 625, 814
MmeI	TCCRAC	2 cut(s)	453, 716
Mox20I	TGGCCA	1 cut(s)	1866
Mph1103I	ATGCAT	1 cut(s)	1857
MroXI	GAANNNNTTC	1 cut(s)	1212
MscI	TGGCCA	1 cut(s)	1866
MseI	TTAA	9 cut(s)	891, 906, 918, 980, 1149, 1524, 1677, 1800, 2094
MslI	CAYNNNNRTG	1 cut(s)	1973
Msp20I	TGGCCA	1 cut(s)	1866
MspA1I	CMGCKG	2 cut(s)	378, 2050
MspCI	CTTAAG	1 cut(s)	1676
MspI	CCGG	1 cut(s)	23
MspR9I	CCNGG	4 cut(s)	637, 794, 1545, 1863
Mva1269I	GAATGC	3 cut(s)	344, 916, 1276
MvaI	CCWGG	4 cut(s)	637, 794, 1545, 1863
MvnI	CGCG	2 cut(s)	191, 378
MwoI	GCNNNNNNNGC	8 cut(s)	60, 71, 243, 350, 647, 1664, 1710, 2104
NarI	GGCGCC	1 cut(s)	63
NdeI	CATATG	1 cut(s)	1385
NdeII	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
NheI	GCTAGC	1 cut(s)	673
NlaIV	GGNNCC	4 cut(s)	64, 161, 694, 1987
NmeAIII	GCCGAG	1 cut(s)	50
NmuCI	GTSAC	5 cut(s)	116, 277, 772, 1442, 1547
NsiI	ATGCAT	1 cut(s)	1857
NspI	RCATGY	4 cut(s)	1102, 1609, 1730, 1859
PaeI	GCATGC	2 cut(s)	1609, 1730
PasI	CCCWGGG	1 cut(s)	636
PciSI	GCTCTTC	1 cut(s)	200
PcsI	WCGNNNNNNNCGW	2 cut(s)	234, 273
PctI	GAATGC	3 cut(s)	344, 916, 1276
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	8 cut(s)	124, 490, 592, 827, 900, 1265, 1978, 2063
PfoI	TCCNGGA	1 cut(s)	792
PleI	GAGTC	4 cut(s)	131, 258, 625, 813
PluTI	GGCGCC	1 cut(s)	66
PpsI	GAGTC	4 cut(s)	131, 258, 625, 813
PpuMI	RGGWCCY	2 cut(s)	1541, 1672
Psp124BI	GAGCTC	1 cut(s)	1493
Psp5II	RGGWCCY	2 cut(s)	1541, 1672
Psp6I	CCWGG	4 cut(s)	635, 792, 1543, 1861
PspEI	GGTNACC	1 cut(s)	1442
PspFI	CCCAGC	1 cut(s)	356
PspGI	CCWGG	4 cut(s)	635, 792, 1543, 1861
PspN4I	GGNNCC	4 cut(s)	64, 161, 694, 1987
PspPI	GGNCC	6 cut(s)	538, 811, 834, 1541, 1672, 1986
PspPPI	RGGWCCY	2 cut(s)	1541, 1672
PstNI	CAGNNNCTG	1 cut(s)	647
PsuI	RGATCY	2 cut(s)	382, 1909
PvuII	CAGCTG	1 cut(s)	2050
RsaI	GTAC	4 cut(s)	653, 956, 1739, 1860
RsaNI	GTAC	4 cut(s)	652, 955, 1738, 1859
RseI	CAYNNNNRTG	1 cut(s)	1973
SacI	GAGCTC	1 cut(s)	1493
SacII	CCGCGG	1 cut(s)	379
SapI	GCTCTTC	1 cut(s)	200
SaqAI	TTAA	9 cut(s)	891, 906, 918, 980, 1149, 1524, 1677, 1800, 2094
Sau3AI	GATC	6 cut(s)	382, 405, 442, 1089, 1909, 2056
Sau96I	GGNCC	6 cut(s)	538, 811, 834, 1541, 1672, 1986
ScaI	AGTACT	1 cut(s)	956
SchI	GAGTC	4 cut(s)	131, 258, 625, 814
ScrFI	CCNGG	4 cut(s)	637, 794, 1545, 1863
SduI	GDGCHC	4 cut(s)	93, 1022, 1493, 1636
SexAI	ACCWGGT	1 cut(s)	1543
SfaNI	GCATC	7 cut(s)	192, 856, 1189, 1369, 1449, 1575, 2070
SfcI	CTRYAG	3 cut(s)	784, 1721, 2118
SfoI	GGCGCC	1 cut(s)	64
Sfr303I	CCGCGG	1 cut(s)	379
SgrBI	CCGCGG	1 cut(s)	379
SinI	GGWCC	3 cut(s)	811, 1541, 1672
SmiMI	CAYNNNNRTG	1 cut(s)	1973
SmlI	CTYRAG	3 cut(s)	422, 1484, 1676
SmoI	CTYRAG	3 cut(s)	422, 1484, 1676
SpeI	ACTAGT	1 cut(s)	2069
SphI	GCATGC	2 cut(s)	1609, 1730
Sse9I	AATT	6 cut(s)	29, 462, 600, 655, 725, 2006
SspDI	GGCGCC	1 cut(s)	62
SspI	AATATT	1 cut(s)	573
SspMI	CTAG	7 cut(s)	51, 363, 567, 674, 1538, 1596, 2070
SstI	GAGCTC	1 cut(s)	1493
StyD4I	CCNGG	4 cut(s)	635, 792, 1543, 1861
TaaI	ACNGT	3 cut(s)	734, 847, 1627
TaiI	ACGT	2 cut(s)	118, 1213
TaqI	TCGA	7 cut(s)	129, 201, 441, 1134, 1290, 1663, 1949
TasI	AATT	6 cut(s)	29, 462, 600, 655, 725, 2006
TatI	WGTACW	1 cut(s)	954
TauI	GCSGC	6 cut(s)	57, 77, 191, 237, 378, 724
TfiI	GAWTC	8 cut(s)	124, 490, 592, 827, 900, 1265, 1978, 2063
Tru1I	TTAA	9 cut(s)	891, 906, 918, 980, 1149, 1524, 1677, 1800, 2094
Tru9I	TTAA	9 cut(s)	891, 906, 918, 980, 1149, 1524, 1677, 1800, 2094
TscAI	CASTG	1 cut(s)	752
TseFI	GTSAC	5 cut(s)	116, 277, 772, 1442, 1547
TseI	GCWGC	6 cut(s)	449, 647, 929, 1312, 1784, 2012
Tsp45I	GTSAC	5 cut(s)	116, 277, 772, 1442, 1547
TspDTI	ATGAA	9 cut(s)	187, 337, 658, 1136, 1269, 1275, 1317, 1765, 1991
TspRI	CASTG	1 cut(s)	752
Vha464I	CTTAAG	1 cut(s)	1676
VpaK11BI	GGWCC	3 cut(s)	811, 1541, 1672
XapI	RAATTY	2 cut(s)	29, 2006
XceI	RCATGY	4 cut(s)	1102, 1609, 1730, 1859
XcmI	CCANNNNNNNNNTGG	1 cut(s)	532
XmiI	GTMKAC	2 cut(s)	7, 84
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	7 cut(s)	51, 363, 567, 674, 1538, 1596, 2070
ZraI	GACGTC	1 cut(s)	116
ZrmI	AGTACT	1 cut(s)	956
Zsp2I	ATGCAT	1 cut(s)	1857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.