Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0007567

Physical Location & Seq

Genomic Coordinates Reverse (-)

3735836 .. 3739631

Gene Structure 3796 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1056500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGGTTGCATCAGCTCCAAGAGGGCGGCTGCCACGGCTTCTCCTTGTTACGAGTACTCGGCCGCAGTTTCCCACAATGGCTCCGTCCTCCTCCAGCCCTCTTCATCTTCCTTGAAAAAGGACAACTCCGCCTCCGCCGCCACCACCAACAACAACGTCAGCCGATCCAAATCCAAAGCAAAGTCCAAATCGAACTCTTCGGAATTTAAGAAGAGTGGTAGCAAGAAGGACCCCCAGAATCATCGACCGGGCGACGTGTCGGAGGATCGGAGCGTCGTCTGCGGGAGAGAGTCGAAGAAGAGCAGGAAAGAGAAGGAGGGGTCTTATCACAGCCATAATAGTCACAATGGCGGGAAAGGTAGCGCCTTTAGCTTCAGGTTGGGTTTTTCCAGTCGTTCTTCTTTGGAGGCAGAGCAAGTCTCCGCCGGCTGGCCCTCTTGGCTCAGCGCCGCCGCCGGCGAGGTCATTTATGGGTGGATCCCCCTCAAAGCTGATGCTTACGAGAAGTTGGAAAAGATTGGACAAGGTACATACAGCAGTGTGTTCCGAGCTAAGGAAGTCGAAACTGGGAGGATTGTTGCACTCAAGAAAGTGCGATTTGACAATTTTCAACCAGAGAGTATTAGGTTTATGGCTCGAGAGATTATGATTCTTCGCAGGCTTGATCATCCGAACATCATGAAATTGGAGGGCATAATAACATCTCGATCATCAAATAGCATATATCTCGTATTCGAGTACATGGAGCACGATCTTGCAGGACTTGTAGCTAGTCCCGAGATCAAATTCAGCGACATGCAGATTAAATGTTATTTGAGGCAGCTATTATCCGCAGTCGAGCACTGTCACTTAAGAGGTATTATGCATCGAGATATCAAGGCCTCGAATATTTTGGTAAACGACGATGGGATTCTAAAGTTGGGAGATTTTGGATTGGCTAATGTCGTTAACTCAAAGAGCAAGCAACCACTGACAAGTCGTGTGGTGACCTTGTGGTACCGTCCTCCTGAACTTTTGATGGGGGCAACTGAGTATGGAGTTTCGGTGGATCTCTGGAGCGTGGGCTGTGTATTTGCAGAACTGTTTCTAGGAAAGCCTATCCTTAAAGGAAGAACCGAGGTCGAACAACTGCACAAAATTTTCAAGCTTTGTGGTTCGCCTCCTGAAGAATACTGGAAAACGTCAAAGCTTCCTCACGCAACTATGTTTAAACCGCAGCACACTTACGAAAGTTCTCTTAGGGAGAGGTGTAAAGAATTTCCAACTACTGCAGTAGACATGTTAGAGAATCTTCTTTCGGTAGAACCTTACAAACGCGGGACTGTCTCCTCTGCCCTAATGTCCGAGTACTTCAAAACAAAGCCTTATGCATGCGATCCGTCACATTTGCCAAAGTATCCTCCTACCAAAGAAATGGATGCTAAAATTCGGGAGGAATCACAAAGAAAAAAACCTGGTATTCGAGCACGAGAGACAAGGAAACCAAGGAGAGTCCGTAAGGCCTTGCTCGAGTCAAATAGTTCCGCTAAATTGGCACCAAAAGAGGATGGGCAAGACGATCATACTCAATCTGCTCGTAGAATGAATGGTGGTAACCCACTTATCCTGAGGGCGAAAGGAGGGCTTCTGCGCCGCGAATCTAATCTAAAACCGCAATTTGATACAGTTTCGGAGAATTGCGAGTTGAATGCATCACTGGGAGATAGTACATTGTCTTCCGTACAAACCTCAGCAGCAAGCGGTTTTGCATGGGCTAAAAGGGGAACTGAGGATGGTACTCAATCCGCAGTTTCAGACGGCTCGAAAAGTCAGTTCAGTGCACTTGATGCAAATTTCGCACGGAGTACATATGACCTAAAACAAGGACATAGAACTGGCAGGATGGCTATACGAAAGCTTAGAACTTTCGACGCGTCTGAACTTACTAGTACAGAAGAGCCAGATGATACCGGAAACAACACGGATTACATTGCGGAAGAGAAGCGTGACATTGAGTTTTCCGGACCCCTACTATCCCAACCGTGTAGAATCGATGAACACTTGCGAAGGAACGAAAGCCATATACGCCATGTAGCTCGACGATCAAGGTTTGAAAAAGAAGTAGAACCCGAGTCTTTACAAGTTGCCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase WRKY

Protein Analysis

711

Amino Acids

79.19

Weight (kDa)

9.33

Isoelectric Point (pI)

49.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	167 - 451	1.3e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	169 - 380	8.2e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007567)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01085 AT3G01085 AT3G01085 AT5G39420
fragaria_vesca	FvH4_6g47810 FvH4_6g47810
malus_domestica	MD09G1056500.v1.1 MD17G1054000.v1.1
prunus_persica	Prupe.3G262200_v2.0.a1 Prupe.3G262200_v2.0.a1
pyrus_communis	pycom111g04730
rosa_chinensis	RchiOBHm_Chr2g0167001
rosa_laevigata	RLG00000021688
rosa_multiflora	Rmu_co8508959.1_g000001
rosa_roxburghii	Rroxscaffold_2G00083820 Rroxscaffold_2G00083870
rosa_rugosa	Rorug02G0527400 Rorug02G0527500 Rorug02G0527600.1
rosa_samantha	Rh2AG594200 Rh2BG605700 Rh2CG576600 Rh2DG617200
rosa_wichuraiana	Rw2G049480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	996
AccB1I	GGYRCC	2 cut(s)	996, 1534
AccI	GTMKAC	1 cut(s)	1275
AccII	CGCG	3 cut(s)	1317, 1635, 1913
AccIII	TCCGGA	1 cut(s)	2000
AclWI	GGATC	6 cut(s)	159, 273, 472, 485, 1056, 1370
AcoI	YGGCCR	1 cut(s)	60
AcsI	RAATTY	6 cut(s)	203, 785, 1137, 1256, 1425, 1831
AcuI	CTGAAG	2 cut(s)	358, 1185
AfiI	CCNNNNNNNGG	2 cut(s)	429, 553
AflII	CTTAAG	1 cut(s)	850
AflIII	ACRYGT	3 cut(s)	255, 1278, 1911
AgsI	TTSAA	6 cut(s)	115, 611, 1144, 1354, 1687, 2093
AhlI	ACTAGT	1 cut(s)	1925
AjiI	CACGTC	1 cut(s)	256
AjnI	CCWGG	1 cut(s)	1453
Alw21I	GWGCWC	4 cut(s)	750, 843, 1468, 1822
Alw26I	GTCTC	3 cut(s)	424, 1330, 1466
Alw44I	GTGCAC	1 cut(s)	1818
AlwI	GGATC	6 cut(s)	159, 273, 472, 485, 1056, 1370
Ama87I	CYCGRG	4 cut(s)	636, 776, 1508, 2108
Aor13HI	TCCGGA	1 cut(s)	2000
AoxI	GGCC	4 cut(s)	60, 431, 879, 1500
ApaLI	GTGCAC	1 cut(s)	1818
ApeKI	GCWGC	4 cut(s)	29, 820, 1216, 1733
ApoI	RAATTY	6 cut(s)	203, 785, 1137, 1256, 1425, 1831
Asp700I	GAANNNNTTC	1 cut(s)	1083
Asp718I	GGTACC	1 cut(s)	996
AspLEI	GCGC	3 cut(s)	365, 449, 1632
AspS9I	GGNCC	3 cut(s)	229, 432, 2003
AsuC2I	CCSGG	1 cut(s)	249
AsuHPI	GGTGA	1 cut(s)	997
AvaI	CYCGRG	4 cut(s)	636, 776, 1508, 2108
AvaII	GGWCC	2 cut(s)	229, 2003
AxyI	CCTNAGG	1 cut(s)	1607
BaeGI	GKGCMC	1 cut(s)	1822
BamHI	GGATCC	1 cut(s)	477
BanI	GGYRCC	2 cut(s)	996, 1534
BarI	GAAGNNNNNNTAC	2 cut(s)	1699, 1731
BauI	CACGAG	1 cut(s)	1467
BbsI	GAAGAC	1 cut(s)	1707
Bbv12I	GWGCWC	4 cut(s)	750, 843, 1468, 1822
BbvCI	CCTCAGC	1 cut(s)	1729
BbvI	GCAGC	4 cut(s)	16, 832, 1228, 1745
BccI	CCATC	5 cut(s)	899, 1012, 1541, 1766, 1876
BceAI	ACGGC	2 cut(s)	51, 1813
BcgI	CGANNNNNNTGC	4 cut(s)	709, 743, 1670, 1704
BciT130I	CCWGG	1 cut(s)	1455
BciVI	GTATCC	1 cut(s)	1407
BclI	TGATCA	1 cut(s)	664
BcnI	CCSGG	1 cut(s)	249
BcoDI	GTCTC	3 cut(s)	424, 1330, 1466
BcuI	ACTAGT	1 cut(s)	1925
BfaI	CTAG	3 cut(s)	771, 1088, 1926
BfmI	CTRYAG	1 cut(s)	1269
BfoI	RGCGCY	2 cut(s)	366, 450
BfrI	CTTAAG	1 cut(s)	850
BfuI	GTATCC	1 cut(s)	1407
BglI	GCCNNNNNGGC	1 cut(s)	439
BlpI	GCTNAGC	1 cut(s)	443
BmcAI	AGTACT	2 cut(s)	56, 1349
Bme1390I	CCNGG	2 cut(s)	249, 1455
Bme18I	GGWCC	2 cut(s)	229, 2003
BmeT110I	CYCGRG	4 cut(s)	636, 776, 1508, 2108
BmgBI	CACGTC	1 cut(s)	256
BmgT120I	GGNCC	3 cut(s)	229, 432, 2003
BmiI	GGNNCC	6 cut(s)	82, 231, 479, 998, 1536, 2005
BmrFI	CCNGG	2 cut(s)	249, 1455
BmrI	ACTGGG	2 cut(s)	576, 1706
BmsI	GCATC	6 cut(s)	18, 484, 874, 1408, 1700, 1816
BmuI	ACTGGG	2 cut(s)	576, 1706
BpiI	GAAGAC	1 cut(s)	1707
BplI	GAGNNNNNCTC	2 cut(s)	404, 436
BpmI	CTGGAG	2 cut(s)	77, 1075
Bpu10I	CCTNAGC	2 cut(s)	552, 1729
Bpu1102I	GCTNAGC	1 cut(s)	443
BpuEI	CTTGAG	1 cut(s)	569
BpuMI	CCSGG	1 cut(s)	249
Bsa29I	ATCGAT	1 cut(s)	2031
BsaBI	GATNNNNATC	1 cut(s)	169
BsaJI	CCNNGG	3 cut(s)	33, 1116, 1484
BsaWI	WCCGGW	2 cut(s)	1949, 2000
Bsc4I	CCNNNNNNNGG	2 cut(s)	429, 553
Bse118I	RCCGGY	2 cut(s)	425, 455
Bse1I	ACTGG	5 cut(s)	390, 571, 1178, 1701, 1879
Bse21I	CCTNAGG	1 cut(s)	1607
Bse3DI	GCAATG	1 cut(s)	1968
Bse8I	GATNNNNATC	1 cut(s)	169
BseAI	TCCGGA	1 cut(s)	2000
BseBI	CCWGG	1 cut(s)	1455
BseCI	ATCGAT	1 cut(s)	2031
BseDI	CCNNGG	3 cut(s)	33, 1116, 1484
BseGI	GGATG	5 cut(s)	667, 1423, 1552, 1777, 1887
BseJI	GATNNNNATC	1 cut(s)	169
BseLI	CCNNNNNNNGG	2 cut(s)	429, 553
BseMI	GCAATG	1 cut(s)	1968
BseMII	CTCAG	5 cut(s)	457, 1020, 1598, 1743, 1758
BseNI	ACTGG	5 cut(s)	390, 571, 1178, 1701, 1879
BseRI	GAGGAG	2 cut(s)	80, 1318
BseSI	GKGCMC	1 cut(s)	1822
BseX3I	CGGCCG	1 cut(s)	60
BseXI	GCAGC	4 cut(s)	16, 832, 1228, 1745
BsgI	GTGCAG	1 cut(s)	1115
Bsh1236I	CGCG	3 cut(s)	1317, 1635, 1913
Bsh1285I	CGRYCG	2 cut(s)	63, 248
BshFI	GGCC	4 cut(s)	62, 433, 881, 1502
BshNI	GGYRCC	2 cut(s)	996, 1534
BshVI	ATCGAT	1 cut(s)	2031
BsiEI	CGRYCG	2 cut(s)	63, 248
BsiHKAI	GWGCWC	4 cut(s)	750, 843, 1468, 1822
BsiHKCI	CYCGRG	4 cut(s)	636, 776, 1508, 2108
BsiSI	CCGG	5 cut(s)	248, 426, 456, 1950, 2001
BslFI	GGGAC	2 cut(s)	759, 1333
BslI	CCNNNNNNNGG	2 cut(s)	429, 553
BsmAI	GTCTC	3 cut(s)	424, 1330, 1466
BsmFI	GGGAC	2 cut(s)	759, 1333
BsmI	GAATGC	1 cut(s)	1693
BsnI	GGCC	4 cut(s)	62, 433, 881, 1502
BsoBI	CYCGRG	4 cut(s)	636, 776, 1508, 2108
Bsp1286I	GDGCHC	4 cut(s)	750, 843, 1468, 1822
Bsp13I	TCCGGA	1 cut(s)	2000
Bsp1720I	GCTNAGC	1 cut(s)	443
BspANI	GGCC	4 cut(s)	62, 433, 881, 1502
BspCNI	CTCAG	5 cut(s)	456, 1021, 1599, 1742, 1759
BspDI	ATCGAT	1 cut(s)	2031
BspEI	TCCGGA	1 cut(s)	2000
BspFNI	CGCG	3 cut(s)	1317, 1635, 1913
BspHI	TCATGA	1 cut(s)	678
BspLI	GGNNCC	6 cut(s)	82, 231, 479, 998, 1536, 2005
BspMAI	CTGCAG	1 cut(s)	1273
BspPI	GGATC	6 cut(s)	159, 273, 472, 485, 1056, 1370
BspQI	GCTCTTC	2 cut(s)	293, 1929
BspT107I	GGYRCC	2 cut(s)	996, 1534
BspTI	CTTAAG	1 cut(s)	850
BsrDI	GCAATG	1 cut(s)	1968
BsrFI	RCCGGY	2 cut(s)	425, 455
BsrI	ACTGG	5 cut(s)	390, 571, 1178, 1701, 1879
BssAI	RCCGGY	2 cut(s)	425, 455
BssECI	CCNNGG	3 cut(s)	33, 1116, 1484
BssSI	CACGAG	1 cut(s)	1467
BssT1I	CCWWGG	1 cut(s)	1484
Bst2BI	CACGAG	1 cut(s)	1467
Bst2UI	CCWGG	1 cut(s)	1455
Bst4CI	ACNGT	6 cut(s)	845, 1001, 1083, 1324, 1666, 2022
Bst6I	CTCTTC	6 cut(s)	106, 202, 206, 293, 1929, 1971
BstAFI	CTTAAG	1 cut(s)	850
BstAPI	GCANNNNNTGC	1 cut(s)	1826
BstC8I	GCNNGC	7 cut(s)	427, 431, 457, 659, 962, 1372, 1738
BstDEI	CTNAG	8 cut(s)	443, 552, 1029, 1238, 1607, 1729, 1767, 1898
BstDSI	CCRYGG	1 cut(s)	33
BstEII	GGTNACC	2 cut(s)	985, 1592
BstF5I	GGATG	5 cut(s)	667, 1423, 1552, 1777, 1887
BstFNI	CGCG	3 cut(s)	1317, 1635, 1913
BstH2I	RGCGCY	2 cut(s)	366, 450
BstHHI	GCGC	3 cut(s)	365, 449, 1632
BstMAI	GTCTC	3 cut(s)	424, 1330, 1466
BstMCI	CGRYCG	2 cut(s)	63, 248
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 137, 279, 369, 439, 1532, 1826, 1835, 2064
BstNI	CCWGG	1 cut(s)	1455
BstNSI	RCATGY	3 cut(s)	799, 1282, 1374
BstPI	GGTNACC	2 cut(s)	985, 1592
BstSCI	CCNGG	2 cut(s)	247, 1453
BstSFI	CTRYAG	1 cut(s)	1269
BstSLI	GKGCMC	1 cut(s)	1822
BstUI	CGCG	3 cut(s)	1317, 1635, 1913
BstV1I	GCAGC	4 cut(s)	16, 832, 1228, 1745
BstV2I	GAAGAC	1 cut(s)	1707
BstX2I	RGATCY	2 cut(s)	477, 1048
BstXI	CCANNNNNNTGG	1 cut(s)	1414
BstYI	RGATCY	2 cut(s)	477, 1048
BstZI	CGGCCG	1 cut(s)	60
Bsu15I	ATCGAT	1 cut(s)	2031
Bsu36I	CCTNAGG	1 cut(s)	1607
BsuI	GTATCC	1 cut(s)	1407
BsuRI	GGCC	4 cut(s)	62, 433, 881, 1502
BsuTUI	ATCGAT	1 cut(s)	2031
BtgI	CCRYGG	1 cut(s)	33
BtrI	CACGTC	1 cut(s)	256
BtsCI	GGATG	5 cut(s)	667, 1423, 1552, 1777, 1887
BtsI	GCAGTG	1 cut(s)	544
BtsIMutI	CAGTG	5 cut(s)	544, 841, 968, 1694, 1822
Cac8I	GCNNGC	7 cut(s)	427, 431, 457, 659, 962, 1372, 1738
CciI	TCATGA	1 cut(s)	678
CfoI	GCGC	3 cut(s)	365, 449, 1632
Cfr10I	RCCGGY	2 cut(s)	425, 455
Cfr13I	GGNCC	3 cut(s)	229, 432, 2003
ClaI	ATCGAT	1 cut(s)	2031
CseI	GACGC	3 cut(s)	262, 1902, 1919
CsiI	ACCWGGT	1 cut(s)	1453
CspCI	CAANNNNNGTGG	4 cut(s)	963, 998, 1132, 1167
CviAII	CATG	7 cut(s)	679, 742, 796, 1279, 1371, 1749, 2069
DdeI	CTNAG	8 cut(s)	443, 552, 1029, 1238, 1607, 1729, 1767, 1898
DraI	TTTAAA	1 cut(s)	1210
EaeI	YGGCCR	1 cut(s)	60
EagI	CGGCCG	1 cut(s)	60
Eam1104I	CTCTTC	6 cut(s)	106, 202, 206, 293, 1929, 1971
EarI	CTCTTC	6 cut(s)	106, 202, 206, 293, 1929, 1971
EciI	GGCGGA	3 cut(s)	118, 124, 412
EclXI	CGGCCG	1 cut(s)	60
Eco130I	CCWWGG	1 cut(s)	1484
Eco147I	AGGCCT	2 cut(s)	881, 1502
Eco32I	GATATC	1 cut(s)	874
Eco47I	GGWCC	2 cut(s)	229, 2003
Eco52I	CGGCCG	1 cut(s)	60
Eco57I	CTGAAG	2 cut(s)	358, 1185
Eco81I	CCTNAGG	1 cut(s)	1607
Eco88I	CYCGRG	4 cut(s)	636, 776, 1508, 2108
Eco91I	GGTNACC	2 cut(s)	985, 1592
EcoO109I	RGGNCCY	1 cut(s)	229
EcoO65I	GGTNACC	2 cut(s)	985, 1592
EcoRII	CCWGG	1 cut(s)	1453
EcoRV	GATATC	1 cut(s)	874
EcoT14I	CCWWGG	1 cut(s)	1484
EcoT22I	ATGCAT	3 cut(s)	867, 1372, 1693
ErhI	CCWWGG	1 cut(s)	1484
FaeI	CATG	7 cut(s)	682, 745, 799, 1282, 1374, 1752, 2072
FalI	AAGNNNNNCTT	2 cut(s)	1608, 1640
FaqI	GGGAC	2 cut(s)	759, 1333
FatI	CATG	7 cut(s)	678, 741, 795, 1278, 1370, 1748, 2068
FauI	CCCGC	3 cut(s)	275, 344, 1310
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	1 cut(s)	664
FblI	GTMKAC	1 cut(s)	1275
FokI	GGATG	5 cut(s)	654, 1430, 1559, 1784, 1894
FspBI	CTAG	3 cut(s)	771, 1088, 1926
GlaI	GCGC	3 cut(s)	364, 448, 1631
GsuI	CTGGAG	2 cut(s)	77, 1075
HaeII	RGCGCY	2 cut(s)	366, 450
HaeIII	GGCC	4 cut(s)	62, 433, 881, 1502
HapII	CCGG	5 cut(s)	248, 426, 456, 1950, 2001
HgaI	GACGC	3 cut(s)	262, 1902, 1919
HhaI	GCGC	3 cut(s)	365, 449, 1632
Hin1II	CATG	7 cut(s)	682, 745, 799, 1282, 1374, 1752, 2072
Hin6I	GCGC	3 cut(s)	363, 447, 1630
HinP1I	GCGC	3 cut(s)	363, 447, 1630
HincII	GTYRAC	1 cut(s)	949
HindII	GTYRAC	1 cut(s)	949
HindIII	AAGCTT	3 cut(s)	1145, 1187, 1895
HpaI	GTTAAC	1 cut(s)	949
HpaII	CCGG	5 cut(s)	248, 426, 456, 1950, 2001
HphI	GGTGA	1 cut(s)	997
Hpy166II	GTNNAC	4 cut(s)	898, 949, 1276, 1820
Hpy188I	TCNGA	9 cut(s)	202, 262, 270, 548, 672, 1345, 1672, 1795, 1918
Hpy8I	GTNNAC	4 cut(s)	898, 949, 1276, 1820
Hpy99I	CGWCG	5 cut(s)	257, 278, 905, 1913, 2082
HpyAV	CCTTC	3 cut(s)	220, 307, 2040
HpyCH4III	ACNGT	6 cut(s)	845, 1001, 1083, 1324, 1666, 2022
HpyCH4IV	ACGT	3 cut(s)	156, 255, 1181
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 137, 279, 369, 439, 1532, 1826, 1835, 2064
HpyF3I	CTNAG	8 cut(s)	443, 552, 1029, 1238, 1607, 1729, 1767, 1898
HpySE526I	ACGT	3 cut(s)	156, 255, 1181
Hsp92II	CATG	7 cut(s)	682, 745, 799, 1282, 1374, 1752, 2072
HspAI	GCGC	3 cut(s)	363, 447, 1630
Kpn2I	TCCGGA	1 cut(s)	2000
KpnI	GGTACC	1 cut(s)	1000
KroI	GCCGGC	2 cut(s)	425, 455
KroNI	GCCGGC	2 cut(s)	427, 457
Ksp22I	TGATCA	1 cut(s)	664
KspAI	GTTAAC	1 cut(s)	949
LguI	GCTCTTC	2 cut(s)	293, 1929
LmnI	GCTCC	5 cut(s)	20, 86, 270, 745, 1056
Lsp1109I	GCAGC	4 cut(s)	16, 832, 1228, 1745
LweI	GCATC	6 cut(s)	18, 484, 874, 1408, 1700, 1816
MabI	ACCWGGT	1 cut(s)	1453
MaeI	CTAG	3 cut(s)	771, 1088, 1926
MaeII	ACGT	3 cut(s)	156, 255, 1181
MaeIII	GTNAC	7 cut(s)	47, 341, 845, 985, 1380, 1592, 1985
MflI	RGATCY	2 cut(s)	477, 1048
MhlI	GDGCHC	4 cut(s)	750, 843, 1468, 1822
MluI	ACGCGT	1 cut(s)	1911
MlyI	GAGTC	4 cut(s)	299, 1500, 1520, 2120
MmeI	TCCRAC	3 cut(s)	240, 489, 1286
Mph1103I	ATGCAT	3 cut(s)	867, 1372, 1693
MreI	CGCCGGCG	1 cut(s)	455
MroI	TCCGGA	1 cut(s)	2000
MroNI	GCCGGC	2 cut(s)	425, 455
MroXI	GAANNNNTTC	1 cut(s)	1083
MseI	TTAA	6 cut(s)	207, 804, 851, 948, 1104, 1209
MspCI	CTTAAG	1 cut(s)	850
MspI	CCGG	5 cut(s)	248, 426, 456, 1950, 2001
MspR9I	CCNGG	2 cut(s)	249, 1455
MssI	GTTTAAAC	1 cut(s)	1210
Mva1269I	GAATGC	1 cut(s)	1693
MvaI	CCWGG	1 cut(s)	1455
MvnI	CGCG	3 cut(s)	1317, 1635, 1913
MwoI	GCNNNNNNNGC	9 cut(s)	35, 137, 279, 369, 439, 1532, 1826, 1835, 2064
NaeI	GCCGGC	2 cut(s)	427, 457
NciI	CCSGG	1 cut(s)	249
NdeI	CATATG	1 cut(s)	1849
NgoMIV	GCCGGC	2 cut(s)	425, 455
NlaIII	CATG	7 cut(s)	682, 745, 799, 1282, 1374, 1752, 2072
NlaIV	GGNNCC	6 cut(s)	82, 231, 479, 998, 1536, 2005
NmeAIII	GCCGAG	1 cut(s)	38
NmuCI	GTSAC	5 cut(s)	341, 845, 985, 1380, 1985
NsiI	ATGCAT	3 cut(s)	867, 1372, 1693
NspI	RCATGY	3 cut(s)	799, 1282, 1374
PaeI	GCATGC	1 cut(s)	1374
PaeR7I	CTCGAG	2 cut(s)	636, 1508
PagI	TCATGA	1 cut(s)	678
PceI	AGGCCT	2 cut(s)	881, 1502
PciI	ACATGT	1 cut(s)	1278
PciSI	GCTCTTC	2 cut(s)	293, 1929
PctI	GAATGC	1 cut(s)	1693
PdiI	GCCGGC	2 cut(s)	427, 457
PdmI	GAANNNNTTC	1 cut(s)	1083
PfeI	GAWTC	7 cut(s)	238, 649, 910, 1288, 1436, 1637, 2028
PleI	GAGTC	4 cut(s)	298, 1499, 1519, 2119
PmeI	GTTTAAAC	1 cut(s)	1210
PpsI	GAGTC	4 cut(s)	298, 1499, 1519, 2119
PpuMI	RGGWCCY	1 cut(s)	229
PscI	ACATGT	1 cut(s)	1278
Psp5II	RGGWCCY	1 cut(s)	229
Psp6I	CCWGG	1 cut(s)	1453
PspEI	GGTNACC	2 cut(s)	985, 1592
PspGI	CCWGG	1 cut(s)	1453
PspN4I	GGNNCC	6 cut(s)	82, 231, 479, 998, 1536, 2005
PspPI	GGNCC	3 cut(s)	229, 432, 2003
PspPPI	RGGWCCY	1 cut(s)	229
PspXI	VCTCGAGB	1 cut(s)	1508
PstI	CTGCAG	1 cut(s)	1273
PsuI	RGATCY	2 cut(s)	477, 1048
SapI	GCTCTTC	2 cut(s)	293, 1929
SaqAI	TTAA	6 cut(s)	207, 804, 851, 948, 1104, 1209
Sau96I	GGNCC	3 cut(s)	229, 432, 2003
ScaI	AGTACT	2 cut(s)	56, 1349
SchI	GAGTC	4 cut(s)	299, 1500, 1520, 2120
ScrFI	CCNGG	2 cut(s)	249, 1455
SduI	GDGCHC	4 cut(s)	750, 843, 1468, 1822
SexAI	ACCWGGT	1 cut(s)	1453
SfaNI	GCATC	6 cut(s)	18, 484, 874, 1408, 1700, 1816
SfcI	CTRYAG	1 cut(s)	1269
Sfr274I	CTCGAG	2 cut(s)	636, 1508
SgrAI	CRCCGGYG	1 cut(s)	455
SinI	GGWCC	2 cut(s)	229, 2003
SlaI	CTCGAG	2 cut(s)	636, 1508
SmlI	CTYRAG	4 cut(s)	584, 636, 850, 1508
SmoI	CTYRAG	4 cut(s)	584, 636, 850, 1508
SpeI	ACTAGT	1 cut(s)	1925
SphI	GCATGC	1 cut(s)	1374
SseBI	AGGCCT	2 cut(s)	881, 1502
SspI	AATATT	1 cut(s)	889
SspMI	CTAG	3 cut(s)	771, 1088, 1926
StuI	AGGCCT	2 cut(s)	881, 1502
StyD4I	CCNGG	2 cut(s)	247, 1453
StyI	CCWWGG	1 cut(s)	1484
TaaI	ACNGT	6 cut(s)	845, 1001, 1083, 1324, 1666, 2022
TaiI	ACGT	3 cut(s)	159, 258, 1184
TatI	WGTACW	6 cut(s)	54, 738, 1347, 1706, 1844, 1928
TauI	GCSGC	6 cut(s)	29, 65, 140, 452, 455, 1635
TfiI	GAWTC	7 cut(s)	238, 649, 910, 1288, 1436, 1637, 2028
Tru1I	TTAA	6 cut(s)	207, 804, 851, 948, 1104, 1209
Tru9I	TTAA	6 cut(s)	207, 804, 851, 948, 1104, 1209
TscAI	CASTG	5 cut(s)	544, 848, 975, 1701, 1822
TseFI	GTSAC	5 cut(s)	341, 845, 985, 1380, 1985
TseI	GCWGC	4 cut(s)	29, 820, 1216, 1733
Tsp45I	GTSAC	5 cut(s)	341, 845, 985, 1380, 1985
TspDTI	ATGAA	4 cut(s)	93, 695, 1598, 2049
TspGWI	ACGGA	6 cut(s)	73, 1368, 1484, 1708, 1855, 1976
TspRI	CASTG	5 cut(s)	544, 848, 975, 1701, 1822
Vha464I	CTTAAG	1 cut(s)	850
VneI	GTGCAC	1 cut(s)	1818
VpaK11BI	GGWCC	2 cut(s)	229, 2003
XapI	RAATTY	6 cut(s)	203, 785, 1137, 1256, 1425, 1831
XceI	RCATGY	3 cut(s)	799, 1282, 1374
XhoI	CTCGAG	2 cut(s)	636, 1508
XmiI	GTMKAC	1 cut(s)	1275
XmnI	GAANNNNTTC	1 cut(s)	1083
XspI	CTAG	3 cut(s)	771, 1088, 1926
ZrmI	AGTACT	2 cut(s)	56, 1349
Zsp2I	ATGCAT	3 cut(s)	867, 1372, 1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.