Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007567

Physical Location & Seq

Genomic Coordinates Reverse (-)

4192384 .. 4196638

Gene Structure 4255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1054000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGGTTGCATCAGCTCCAAGCGGGCGGCTGCCTCGGCGTCTCCTTGTTACGAGTACTCGGCCGCCGTCGCCGGCAATGGCTCCGTCCTCCTCGAGCCCTCTTCGTCCTCCTTAAAAAAGAACAACTCCGCCTCCGCTGCCAGCAACAGCAACAACGTTAGCCGATCCAAATCCAAACCAAAGTCCAAATCGAACTCTTCGGAATTTAAGAAGAGTGGTAGCAAGAGGGAACCACAGAATCAGAATCATCGTCCGGGCGACGTGTCGGAGGATCGGAGTGTCGTTGGCGGCAGACAGTCGAAGAAGAGCAGGAAGGAGAAGGAGGGGTCTTATCACAGCCATAATAGTCACAATGGCGGGAAAGGTAGTGCCTTTAGCTTCAGATTGGGTTTTTCAAGTCGTTCTTCTTTGGAGGCTGAGCAAGTCTCCGCCGGCTGGCCCTCTTGGCTCAGCGCCGCAGCCGGCGAGGCCATATACGGGTGGATCCCCCTCAAAGCTGATGCTTATGAGAAGTTGGAAAAGATTGGACAAGGTACGTACAGCAGTGTGTTCCGAGCAAAGGAAGTGGAAACTGGGAGGATTGTTGCACTCAAGAAAGTACGTTTTGACAATTTTCAACCGGAGAGTATTAGGTTTATGGCTAGAGAGATCATGATTCTTCGCAGGCTTGATCATCCGAACATCATGAAACTGGACGGAATAATAACATCTCGATCATCAAATAGCATATACCTTGTATTCGAGTACATGGAGCACGATCTTGCAGGACTTGTAGCTAGTTCCGAGATCAAATTCACTGATATGCAGATTAAATGTTATATGAGGCAGCTATTTTCCGCAGTCGAGCACTGTCACTTAAGAGGTATTATGCATAGAGACATCAAAGCCTCCAATATTTTGGTAAACAACGAAGGGATTCTGAAGTTGGGAGATTTTGGATTGGCGAATGTCATTAACTCAAAGAGCAAGCGACCGCTGACCAGTCGGGTGGTGACCTTGTGGTACCGTCCTCCTGAACTTTTGATGGGGGCAACTGAGTATGGAGTTTCGGTGGATCTCTGGAGCGTGGGCTGCGTATTTGCAGAGCTGTTTCTAGGAAAGACTCTCCTTAAAGGAAGAACTGAGGTCGAACAATTGCACAAAATTTTCAAGCTTTGTGGTTCACCGCCTGAAGAATACTGGAAAACGTCAAAACTTCCTCACGCAACTATGTTTAAACCACAGTACAACTACGAAAGTTCTCTTAAGGAGAGGTGTAAAGAGTTTCCAACTACTGCACTAGACATGTTAGAGAATCTCCTTTCCGTAGAACCTTACAAGCGCGGGACTGCCTCCTCTGCCCTAATGTCCGAGTACTTCAAAACAAAGCCTTATGCCTGCGATCCGTCACATTTGCCAAAGTATCCTCCTACCAAAGAAATGGATGCTAAAAATCGGGAGGAATCACAAAGGAAAAGACCTGGTATTCGAGCACGAGAGACAACAAGGAAACCAAGGAGAGTCCGTAAGGCCTTGCACGAGTCAAATGGTTCCGCTAAAGTGGCACCAAAAGAGGATGGGCAAGACGATCATACTCAATCTGCTCGTCGAATCAATGGTGGTAACCCGTTTATCCCAAAGGCGAAAGGAGGGCTTCTGCGCCGAGAGTCTAATCTAAAACCGCAATTTGATACAGTTTCGGAGAATTGCGAGTTGAATGCATCACAAGGATACAGTACATTGTCTTCCGTACAAACCTCAGCAGCAAGCGGCTTTGCATGGGCAAAAAGGGGAACAGAGGATGGTACTAAATCTGCAGTTTCAGATGGCTCGAAAAGCCAGTTCAGTGCCCTCGATGCAAACTTCGCAAAGAGTACATTTGACCTAAAACAAGGATACAGAACTGGCAAGACTGCGATACGAAAGCTTAGAACTTTCGACACGTCTGAACTTGCTAGTACAGGAGAGCCAGACGATACAGGAAACGACATGGATTCCGTTGTGGAAGAGAACCGTGCCATTGAGTTTTCCGGACCCCTACTTTCCCAACAGTGTGGAATCGACGAACACTTGCAAAGGAACGAAAGCCATATACGCCATGTATCTCGAAGATCAAGGTTCGAAAGAGAGGTAGAACCCCAACCTTTACAGGTGACCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase WRKY

Protein Analysis

714

Amino Acids

79.28

Weight (kDa)

9.46

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	169 - 453	5.4e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	171 - 382	4e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007567)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01085 AT3G01085 AT3G01085 AT5G39420
fragaria_vesca	FvH4_6g47810 FvH4_6g47810
malus_domestica	MD09G1056500.v1.1 MD17G1054000.v1.1
prunus_persica	Prupe.3G262200_v2.0.a1 Prupe.3G262200_v2.0.a1
pyrus_communis	pycom111g04730
rosa_chinensis	RchiOBHm_Chr2g0167001
rosa_laevigata	RLG00000021688
rosa_multiflora	Rmu_co8508959.1_g000001
rosa_roxburghii	Rroxscaffold_2G00083820 Rroxscaffold_2G00083870
rosa_rugosa	Rorug02G0527400 Rorug02G0527500 Rorug02G0527600.1
rosa_samantha	Rh2AG594200 Rh2BG605700 Rh2CG576600 Rh2DG617200
rosa_wichuraiana	Rw2G049480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1002
AccB1I	GGYRCC	2 cut(s)	1002, 1543
AccII	CGCG	1 cut(s)	1323
AccIII	TCCGGA	1 cut(s)	2009
AclI	AACGTT	1 cut(s)	156
AclWI	GGATC	6 cut(s)	159, 279, 478, 491, 1062, 1376
AcoI	YGGCCR	1 cut(s)	60
AcsI	RAATTY	3 cut(s)	203, 791, 1143
AcuI	CTGAAG	3 cut(s)	364, 941, 1191
AcyI	GRCGYC	1 cut(s)	38
AfiI	CCNNNNNNNGG	3 cut(s)	435, 477, 559
AflII	CTTAAG	2 cut(s)	856, 1244
AflIII	ACRYGT	3 cut(s)	261, 1284, 1920
AgsI	TTSAA	5 cut(s)	396, 617, 1150, 1360, 1696
AjiI	CACGTC	2 cut(s)	262, 1923
AjnI	CCWGG	1 cut(s)	1459
AluBI	AGCT	8 cut(s)	15, 378, 497, 776, 829, 1087, 1153, 1906
AluI	AGCT	8 cut(s)	15, 378, 497, 776, 829, 1087, 1153, 1906
Alw21I	GWGCWC	3 cut(s)	756, 849, 1474
Alw26I	GTCTC	4 cut(s)	45, 430, 870, 1472
AlwI	GGATC	6 cut(s)	159, 279, 478, 491, 1062, 1376
Ama87I	CYCGRG	1 cut(s)	92
Aor13HI	TCCGGA	1 cut(s)	2009
AoxI	GGCC	4 cut(s)	60, 437, 468, 1509
ApeKI	GCWGC	6 cut(s)	29, 137, 458, 826, 1071, 1742
ApoI	RAATTY	3 cut(s)	203, 791, 1143
ArsI	GACNNNNNNTTYG	2 cut(s)	1449, 1481
Asp700I	GAANNNNTTC	1 cut(s)	915
Asp718I	GGTACC	1 cut(s)	1002
AspLEI	GCGC	3 cut(s)	455, 1323, 1641
AspS9I	GGNCC	2 cut(s)	438, 2012
AsuC2I	CCSGG	1 cut(s)	255
AsuHPI	GGTGA	2 cut(s)	1003, 1155
AsuII	TTCGAA	1 cut(s)	2100
AvaI	CYCGRG	1 cut(s)	92
AvaII	GGWCC	1 cut(s)	2012
BaeGI	GKGCMC	1 cut(s)	1831
BamHI	GGATCC	1 cut(s)	483
BanI	GGYRCC	2 cut(s)	1002, 1543
BanII	GRGCYC	1 cut(s)	99
BarI	GAAGNNNNNNTAC	2 cut(s)	1708, 1740
BauI	CACGAG	2 cut(s)	1473, 1517
BbsI	GAAGAC	1 cut(s)	1716
Bbv12I	GWGCWC	3 cut(s)	756, 849, 1474
BbvCI	CCTCAGC	1 cut(s)	1738
BbvI	GCAGC	6 cut(s)	16, 124, 470, 838, 1058, 1754
BccI	CCATC	4 cut(s)	1018, 1550, 1775, 1799
BceAI	ACGGC	1 cut(s)	50
BcgI	CGANNNNNNTGC	2 cut(s)	1679, 1713
BciT130I	CCWGG	1 cut(s)	1461
BciVI	GTATCC	3 cut(s)	1413, 1703, 1868
BclI	TGATCA	1 cut(s)	670
BcnI	CCSGG	1 cut(s)	255
BcoDI	GTCTC	4 cut(s)	45, 430, 870, 1472
BfaI	CTAG	5 cut(s)	642, 777, 1094, 1280, 1935
BfmI	CTRYAG	1 cut(s)	1794
BfoI	RGCGCY	1 cut(s)	456
BfrI	CTTAAG	2 cut(s)	856, 1244
BfuI	GTATCC	3 cut(s)	1413, 1703, 1868
BglI	GCCNNNNNGGC	2 cut(s)	445, 467
BlpI	GCTNAGC	2 cut(s)	417, 449
BmcAI	AGTACT	2 cut(s)	56, 1355
Bme1390I	CCNGG	2 cut(s)	255, 1461
Bme18I	GGWCC	1 cut(s)	2012
BmeT110I	CYCGRG	1 cut(s)	92
BmgBI	CACGTC	2 cut(s)	262, 1923
BmgT120I	GGNCC	2 cut(s)	438, 2012
BmiI	GGNNCC	7 cut(s)	82, 231, 485, 1004, 1531, 1545, 2014
BmrFI	CCNGG	2 cut(s)	255, 1461
BmrI	ACTGGG	1 cut(s)	582
BmsI	GCATC	5 cut(s)	18, 490, 1414, 1709, 1825
BmuI	ACTGGG	1 cut(s)	582
BpiI	GAAGAC	1 cut(s)	1716
BplI	GAGNNNNNCTC	2 cut(s)	410, 442
BpmI	CTGGAG	1 cut(s)	1081
Bpu10I	CCTNAGC	1 cut(s)	1738
Bpu1102I	GCTNAGC	2 cut(s)	417, 449
Bpu14I	TTCGAA	1 cut(s)	2100
BpuEI	CTTGAG	1 cut(s)	575
BpuMI	CCSGG	1 cut(s)	255
BsaAI	YACGTR	1 cut(s)	537
BsaBI	GATNNNNATC	1 cut(s)	169
BsaHI	GRCGYC	1 cut(s)	38
BsaJI	CCNNGG	2 cut(s)	33, 1493
BsaWI	WCCGGW	2 cut(s)	619, 2009
BsaXI	ACNNNNNCTCC	6 cut(s)	116, 146, 1035, 1065, 1489, 1519
Bsc4I	CCNNNNNNNGG	3 cut(s)	435, 477, 559
Bse118I	RCCGGY	3 cut(s)	71, 431, 461
Bse1I	ACTGG	6 cut(s)	577, 696, 981, 1184, 1819, 1888
Bse3DI	GCAATG	1 cut(s)	82
Bse8I	GATNNNNATC	1 cut(s)	169
BseAI	TCCGGA	1 cut(s)	2009
BseBI	CCWGG	1 cut(s)	1461
BseDI	CCNNGG	2 cut(s)	33, 1493
BseGI	GGATG	4 cut(s)	673, 1429, 1561, 1786
BseJI	GATNNNNATC	1 cut(s)	169
BseLI	CCNNNNNNNGG	3 cut(s)	435, 477, 559
BseMI	GCAATG	1 cut(s)	82
BseMII	CTCAG	5 cut(s)	408, 463, 1026, 1113, 1752
BseNI	ACTGG	6 cut(s)	577, 696, 981, 1184, 1819, 1888
BseRI	GAGGAG	2 cut(s)	80, 1324
BseSI	GKGCMC	1 cut(s)	1831
BseX3I	CGGCCG	1 cut(s)	60
BseXI	GCAGC	6 cut(s)	16, 124, 470, 838, 1058, 1754
BsgI	GTGCAG	1 cut(s)	1260
Bsh1236I	CGCG	1 cut(s)	1323
Bsh1285I	CGRYCG	2 cut(s)	63, 974
BshFI	GGCC	4 cut(s)	62, 439, 470, 1511
BshNI	GGYRCC	2 cut(s)	1002, 1543
BsiEI	CGRYCG	2 cut(s)	63, 974
BsiHKAI	GWGCWC	3 cut(s)	756, 849, 1474
BsiHKCI	CYCGRG	1 cut(s)	92
BsiSI	CCGG	6 cut(s)	72, 254, 432, 462, 620, 2010
BslFI	GGGAC	1 cut(s)	1339
BslI	CCNNNNNNNGG	3 cut(s)	435, 477, 559
BsmAI	GTCTC	4 cut(s)	45, 430, 870, 1472
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	1339
BsmI	GAATGC	1 cut(s)	1702
BsnI	GGCC	4 cut(s)	62, 439, 470, 1511
BsoBI	CYCGRG	1 cut(s)	92
Bsp119I	TTCGAA	1 cut(s)	2100
Bsp1286I	GDGCHC	5 cut(s)	99, 756, 849, 1474, 1831
Bsp13I	TCCGGA	1 cut(s)	2009
Bsp1720I	GCTNAGC	2 cut(s)	417, 449
BspANI	GGCC	4 cut(s)	62, 439, 470, 1511
BspCNI	CTCAG	5 cut(s)	409, 462, 1027, 1114, 1751
BspEI	TCCGGA	1 cut(s)	2009
BspFNI	CGCG	1 cut(s)	1323
BspHI	TCATGA	2 cut(s)	651, 684
BspLI	GGNNCC	7 cut(s)	82, 231, 485, 1004, 1531, 1545, 2014
BspMAI	CTGCAG	1 cut(s)	1798
BspPI	GGATC	6 cut(s)	159, 279, 478, 491, 1062, 1376
BspQI	GCTCTTC	1 cut(s)	299
BspT104I	TTCGAA	1 cut(s)	2100
BspT107I	GGYRCC	2 cut(s)	1002, 1543
BspTI	CTTAAG	2 cut(s)	856, 1244
BsrDI	GCAATG	1 cut(s)	82
BsrFI	RCCGGY	3 cut(s)	71, 431, 461
BsrI	ACTGG	6 cut(s)	577, 696, 981, 1184, 1819, 1888
BssAI	RCCGGY	3 cut(s)	71, 431, 461
BssECI	CCNNGG	2 cut(s)	33, 1493
BssNI	GRCGYC	1 cut(s)	38
BssSI	CACGAG	2 cut(s)	1473, 1517
BssT1I	CCWWGG	1 cut(s)	1493
Bst2BI	CACGAG	2 cut(s)	1473, 1517
Bst2UI	CCWGG	1 cut(s)	1461
Bst4CI	ACNGT	8 cut(s)	297, 851, 1007, 1224, 1675, 1715, 1994, 2031
Bst6I	CTCTTC	5 cut(s)	106, 202, 206, 299, 1980
BstACI	GRCGYC	1 cut(s)	38
BstAFI	CTTAAG	2 cut(s)	856, 1244
BstBAI	YACGTR	1 cut(s)	537
BstBI	TTCGAA	1 cut(s)	2100
BstDEI	CTNAG	6 cut(s)	417, 449, 1035, 1122, 1738, 1907
BstEII	GGTNACC	3 cut(s)	991, 1601, 2131
BstF5I	GGATG	4 cut(s)	673, 1429, 1561, 1786
BstFNI	CGCG	1 cut(s)	1323
BstH2I	RGCGCY	1 cut(s)	456
BstHHI	GCGC	3 cut(s)	455, 1323, 1641
BstMAI	GTCTC	4 cut(s)	45, 430, 870, 1472
BstMCI	CGRYCG	2 cut(s)	63, 974
BstNI	CCWGG	1 cut(s)	1461
BstNSI	RCATGY	1 cut(s)	1288
BstPI	GGTNACC	3 cut(s)	991, 1601, 2131
BstSCI	CCNGG	2 cut(s)	253, 1459
BstSFI	CTRYAG	1 cut(s)	1794
BstSLI	GKGCMC	1 cut(s)	1831
BstSNI	TACGTA	1 cut(s)	537
BstUI	CGCG	1 cut(s)	1323
BstV1I	GCAGC	6 cut(s)	16, 124, 470, 838, 1058, 1754
BstV2I	GAAGAC	1 cut(s)	1716
BstX2I	RGATCY	2 cut(s)	483, 1054
BstXI	CCANNNNNNTGG	4 cut(s)	898, 988, 1420, 2033
BstYI	RGATCY	2 cut(s)	483, 1054
BstZI	CGGCCG	1 cut(s)	60
BsuI	GTATCC	3 cut(s)	1413, 1703, 1868
BsuRI	GGCC	4 cut(s)	62, 439, 470, 1511
BtrI	CACGTC	2 cut(s)	262, 1923
BtsCI	GGATG	4 cut(s)	673, 1429, 1561, 1786
BtsI	GCAGTG	1 cut(s)	550
BtsIMutI	CAGTG	5 cut(s)	550, 795, 847, 1831, 2036
CciI	TCATGA	2 cut(s)	651, 684
CfoI	GCGC	3 cut(s)	455, 1323, 1641
Cfr10I	RCCGGY	3 cut(s)	71, 431, 461
Cfr13I	GGNCC	2 cut(s)	438, 2012
CseI	GACGC	1 cut(s)	27
CsiI	ACCWGGT	1 cut(s)	1459
CspCI	CAANNNNNGTGG	4 cut(s)	546, 581, 1138, 1173
CviAII	CATG	7 cut(s)	652, 685, 748, 1285, 1758, 1969, 2078
DdeI	CTNAG	6 cut(s)	417, 449, 1035, 1122, 1738, 1907
DraI	TTTAAA	1 cut(s)	1216
EaeI	YGGCCR	1 cut(s)	60
EagI	CGGCCG	1 cut(s)	60
Eam1104I	CTCTTC	5 cut(s)	106, 202, 206, 299, 1980
EarI	CTCTTC	5 cut(s)	106, 202, 206, 299, 1980
EciI	GGCGGA	2 cut(s)	118, 418
EclXI	CGGCCG	1 cut(s)	60
Eco105I	TACGTA	1 cut(s)	537
Eco130I	CCWWGG	1 cut(s)	1493
Eco147I	AGGCCT	1 cut(s)	1511
Eco24I	GRGCYC	1 cut(s)	99
Eco47I	GGWCC	1 cut(s)	2012
Eco52I	CGGCCG	1 cut(s)	60
Eco57I	CTGAAG	3 cut(s)	364, 941, 1191
Eco88I	CYCGRG	1 cut(s)	92
Eco91I	GGTNACC	3 cut(s)	991, 1601, 2131
EcoO65I	GGTNACC	3 cut(s)	991, 1601, 2131
EcoRII	CCWGG	1 cut(s)	1459
EcoT14I	CCWWGG	1 cut(s)	1493
EcoT22I	ATGCAT	2 cut(s)	873, 1702
EcoT38I	GRGCYC	1 cut(s)	99
ErhI	CCWWGG	1 cut(s)	1493
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	7 cut(s)	655, 688, 751, 1288, 1761, 1972, 2081
FalI	AAGNNNNNCTT	4 cut(s)	388, 420, 1617, 1649
FaqI	GGGAC	1 cut(s)	1339
FatI	CATG	7 cut(s)	651, 684, 747, 1284, 1757, 1968, 2077
FauI	CCCGC	3 cut(s)	15, 350, 1316
FauNDI	CATATG	1 cut(s)	2138
FbaI	TGATCA	1 cut(s)	670
FokI	GGATG	4 cut(s)	660, 1436, 1568, 1793
FriOI	GRGCYC	1 cut(s)	99
FspBI	CTAG	5 cut(s)	642, 777, 1094, 1280, 1935
GlaI	GCGC	3 cut(s)	454, 1322, 1640
GsuI	CTGGAG	1 cut(s)	1081
HaeII	RGCGCY	1 cut(s)	456
HaeIII	GGCC	4 cut(s)	62, 439, 470, 1511
HapII	CCGG	6 cut(s)	72, 254, 432, 462, 620, 2010
HgaI	GACGC	1 cut(s)	27
HhaI	GCGC	3 cut(s)	455, 1323, 1641
Hin1I	GRCGYC	1 cut(s)	38
Hin1II	CATG	7 cut(s)	655, 688, 751, 1288, 1761, 1972, 2081
Hin6I	GCGC	3 cut(s)	453, 1321, 1639
HinP1I	GCGC	3 cut(s)	453, 1321, 1639
HindIII	AAGCTT	2 cut(s)	1151, 1904
HpaII	CCGG	6 cut(s)	72, 254, 432, 462, 620, 2010
HphI	GGTGA	2 cut(s)	1003, 1155
Hpy166II	GTNNAC	2 cut(s)	904, 1163
Hpy188III	TCNNGA	9 cut(s)	592, 652, 685, 711, 1013, 1060, 1436, 2010, 2085
Hpy8I	GTNNAC	2 cut(s)	904, 1163
Hpy99I	CGWCG	4 cut(s)	71, 263, 1590, 2045
HpyAV	CCTTC	3 cut(s)	307, 313, 905
HpyCH4III	ACNGT	8 cut(s)	297, 851, 1007, 1224, 1675, 1715, 1994, 2031
HpyCH4IV	ACGT	6 cut(s)	156, 261, 536, 601, 1187, 1922
HpyF3I	CTNAG	6 cut(s)	417, 449, 1035, 1122, 1738, 1907
HpySE526I	ACGT	6 cut(s)	156, 261, 536, 601, 1187, 1922
Hsp92I	GRCGYC	1 cut(s)	38
Hsp92II	CATG	7 cut(s)	655, 688, 751, 1288, 1761, 1972, 2081
HspAI	GCGC	3 cut(s)	453, 1321, 1639
Kpn2I	TCCGGA	1 cut(s)	2009
KpnI	GGTACC	1 cut(s)	1006
KroI	GCCGGC	3 cut(s)	71, 431, 461
KroNI	GCCGGC	3 cut(s)	73, 433, 463
Ksp22I	TGATCA	1 cut(s)	670
LguI	GCTCTTC	1 cut(s)	299
LmnI	GCTCC	4 cut(s)	20, 86, 751, 1062
Lsp1109I	GCAGC	6 cut(s)	16, 124, 470, 838, 1058, 1754
LweI	GCATC	5 cut(s)	18, 490, 1414, 1709, 1825
MabI	ACCWGGT	1 cut(s)	1459
MaeI	CTAG	5 cut(s)	642, 777, 1094, 1280, 1935
MaeII	ACGT	6 cut(s)	156, 261, 536, 601, 1187, 1922
MaeIII	GTNAC	7 cut(s)	47, 347, 851, 991, 1386, 1601, 2131
MfeI	CAATTG	1 cut(s)	1133
MflI	RGATCY	2 cut(s)	483, 1054
MhlI	GDGCHC	5 cut(s)	99, 756, 849, 1474, 1831
MluCI	AATT	7 cut(s)	203, 610, 791, 1133, 1143, 1664, 1684
MlyI	GAGTC	4 cut(s)	1096, 1509, 1529, 1655
MmeI	TCCRAC	3 cut(s)	246, 495, 1292
Mph1103I	ATGCAT	2 cut(s)	873, 1702
MroI	TCCGGA	1 cut(s)	2009
MroNI	GCCGGC	3 cut(s)	71, 431, 461
MroXI	GAANNNNTTC	1 cut(s)	915
MseI	TTAA	8 cut(s)	113, 207, 810, 857, 954, 1110, 1215, 1245
MslI	CAYNNNNRTG	1 cut(s)	800
MspA1I	CMGCKG	2 cut(s)	137, 976
MspCI	CTTAAG	2 cut(s)	856, 1244
MspI	CCGG	6 cut(s)	72, 254, 432, 462, 620, 2010
MspR9I	CCNGG	2 cut(s)	255, 1461
MssI	GTTTAAAC	1 cut(s)	1216
MunI	CAATTG	1 cut(s)	1133
Mva1269I	GAATGC	1 cut(s)	1702
MvaI	CCWGG	1 cut(s)	1461
MvnI	CGCG	1 cut(s)	1323
NaeI	GCCGGC	3 cut(s)	73, 433, 463
NciI	CCSGG	1 cut(s)	255
NdeI	CATATG	1 cut(s)	2138
NgoMIV	GCCGGC	3 cut(s)	71, 431, 461
NlaIII	CATG	7 cut(s)	655, 688, 751, 1288, 1761, 1972, 2081
NlaIV	GGNNCC	7 cut(s)	82, 231, 485, 1004, 1531, 1545, 2014
NmeAIII	GCCGAG	3 cut(s)	14, 38, 1667
NmuCI	GTSAC	5 cut(s)	347, 851, 991, 1386, 2131
NsiI	ATGCAT	2 cut(s)	873, 1702
NspI	RCATGY	1 cut(s)	1288
NspV	TTCGAA	1 cut(s)	2100
PaeR7I	CTCGAG	1 cut(s)	92
PagI	TCATGA	2 cut(s)	651, 684
PceI	AGGCCT	1 cut(s)	1511
PciI	ACATGT	1 cut(s)	1284
PciSI	GCTCTTC	1 cut(s)	299
PctI	GAATGC	1 cut(s)	1702
PdiI	GCCGGC	3 cut(s)	73, 433, 463
PdmI	GAANNNNTTC	1 cut(s)	915
PfeI	GAWTC	9 cut(s)	238, 244, 655, 916, 1294, 1442, 1590, 1973, 2037
PleI	GAGTC	4 cut(s)	1096, 1508, 1528, 1654
PmeI	GTTTAAAC	1 cut(s)	1216
PpsI	GAGTC	4 cut(s)	1096, 1508, 1528, 1654
Ppu21I	YACGTR	1 cut(s)	537
PscI	ACATGT	1 cut(s)	1284
Psp1406I	AACGTT	1 cut(s)	156
Psp6I	CCWGG	1 cut(s)	1459
PspEI	GGTNACC	3 cut(s)	991, 1601, 2131
PspGI	CCWGG	1 cut(s)	1459
PspN4I	GGNNCC	7 cut(s)	82, 231, 485, 1004, 1531, 1545, 2014
PspPI	GGNCC	2 cut(s)	438, 2012
PspXI	VCTCGAGB	1 cut(s)	92
PstI	CTGCAG	1 cut(s)	1798
PsuI	RGATCY	2 cut(s)	483, 1054
RseI	CAYNNNNRTG	1 cut(s)	800
SapI	GCTCTTC	1 cut(s)	299
SaqAI	TTAA	8 cut(s)	113, 207, 810, 857, 954, 1110, 1215, 1245
Sau96I	GGNCC	2 cut(s)	438, 2012
ScaI	AGTACT	2 cut(s)	56, 1355
SchI	GAGTC	4 cut(s)	1096, 1509, 1529, 1655
ScrFI	CCNGG	2 cut(s)	255, 1461
SduI	GDGCHC	5 cut(s)	99, 756, 849, 1474, 1831
SexAI	ACCWGGT	1 cut(s)	1459
SfaNI	GCATC	5 cut(s)	18, 490, 1414, 1709, 1825
SfcI	CTRYAG	1 cut(s)	1794
Sfr274I	CTCGAG	1 cut(s)	92
SfuI	TTCGAA	1 cut(s)	2100
SinI	GGWCC	1 cut(s)	2012
SlaI	CTCGAG	1 cut(s)	92
SmiMI	CAYNNNNRTG	1 cut(s)	800
SmlI	CTYRAG	4 cut(s)	92, 590, 856, 1244
SmoI	CTYRAG	4 cut(s)	92, 590, 856, 1244
SnaBI	TACGTA	1 cut(s)	537
Sse9I	AATT	7 cut(s)	203, 610, 791, 1133, 1143, 1664, 1684
SseBI	AGGCCT	1 cut(s)	1511
SspI	AATATT	1 cut(s)	895
SspMI	CTAG	5 cut(s)	642, 777, 1094, 1280, 1935
StuI	AGGCCT	1 cut(s)	1511
StyD4I	CCNGG	2 cut(s)	253, 1459
StyI	CCWWGG	1 cut(s)	1493
TaaI	ACNGT	8 cut(s)	297, 851, 1007, 1224, 1675, 1715, 1994, 2031
TaiI	ACGT	6 cut(s)	159, 264, 539, 604, 1190, 1925
TasI	AATT	7 cut(s)	203, 610, 791, 1133, 1143, 1664, 1684
TatI	WGTACW	7 cut(s)	54, 744, 1224, 1353, 1715, 1853, 1937
TauI	GCSGC	5 cut(s)	29, 65, 291, 458, 1752
TfiI	GAWTC	9 cut(s)	238, 244, 655, 916, 1294, 1442, 1590, 1973, 2037
Tru1I	TTAA	8 cut(s)	113, 207, 810, 857, 954, 1110, 1215, 1245
Tru9I	TTAA	8 cut(s)	113, 207, 810, 857, 954, 1110, 1215, 1245
TscAI	CASTG	5 cut(s)	550, 802, 854, 1831, 2036
TseFI	GTSAC	5 cut(s)	347, 851, 991, 1386, 2131
TseI	GCWGC	6 cut(s)	29, 137, 458, 826, 1071, 1742
Tsp45I	GTSAC	5 cut(s)	347, 851, 991, 1386, 2131
TspDTI	ATGAA	1 cut(s)	701
TspGWI	ACGGA	7 cut(s)	73, 711, 1294, 1374, 1493, 1717, 1966
TspRI	CASTG	5 cut(s)	550, 802, 854, 1831, 2036
Vha464I	CTTAAG	2 cut(s)	856, 1244
VpaK11BI	GGWCC	1 cut(s)	2012
XapI	RAATTY	3 cut(s)	203, 791, 1143
XceI	RCATGY	1 cut(s)	1288
XhoI	CTCGAG	1 cut(s)	92
XmnI	GAANNNNTTC	1 cut(s)	915
XspI	CTAG	5 cut(s)	642, 777, 1094, 1280, 1935
ZrmI	AGTACT	2 cut(s)	56, 1355
Zsp2I	ATGCAT	2 cut(s)	873, 1702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.