Rorug02G0527400
WRKY Family

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007567
Physical Location & Seq
Forward (+)
65716828 .. 65718416
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0527400.1

Sequence Viewer

Length: 1563 bp
ATGAGGCTTCTTCCCACCTTGACAGTCGGTGGCACACAGCCCCGCCCACCATGGGCGCTTTTGCCCGGATTTTCTCTCCGATCTGTGGCCATGTGCCTCTCTTTGCCTTCCTCTGTTTTTTCCTTTCAGTTGGGTCTCCCTTGTTGTTTCTTTCCTTATGGTTTTCTTGGTTTGTTTCTCATGTTCCTGACCAAATATTTTCCAGATCTACTTTGTTTCCCCTCAAACCCGTCCCTTTACCTGTGCATCGCTATCTCCGGTGATGCTTATCCTAAGCCTACTGCCATGACCTGTAAATATGCTCCTCGGAGCTCATCTCAAAACGGGAGTTGCCCGTTAGTGCACCCGGTGATGCTACAAGGAACTGAGTACCCTTTGGGTGCCTGTCCCTCCGATCCTTTTCTGGATCTCACCTCTCTCTCTTTTCAACTAGGCTATCTGGTTGATTTCTTGGCAGATGAAGATCTTCTTGTTGGAGATGGTTTGAGTTATGTGCTCTTGACTTTTGTATCTGGAAAGTTGGCGGATGGGTACTTCGGAATCGCCATCGATCCCCTCAACGATCTCTCGTTCCGGTTGGATGTTGTTGCTTGTGGCTGCGCTCTACAATGCGGCGGCGCTGCTGGTTCTTCTTCGTTGTCGGCAACTGTGTTGGCTAACCAAGACTGTGGGCTTGCCTGGTTTAGGCCCACTATGTTTGTTGTTTTTCTGTTTTTGTTTGGGCCCTTTGGGCTCTGTTGTCTACTTTTGTTGGCTTCTGGAAGGCCTAGCTGTCCTTGTTTTCTAGTTGTTTGCCAAAAAAAAAAAAAAAAAGGAGCCCTTGGTTGGAATACTTATCGTGCTATTAACGTGAGGTTCGACTACATCTCCAACTCCATAATTCAGGACATAACTTCCCTTAACAGCAGAAGTTACCACATTAACGTTTATGCGTGCCATGATGTTACATTCCAGCGTGTGACTGTCATAGCACCCGGCGAGAGTTTTAATACAGATGGAATCCACATTGCACGTTCATGGAGGATCAACGTAACGGATACAAACATCGGAACCGGAGATGATTGTATTTCCATCGGTGATGGCACTTCGCAACTCATAGTAACCAATGTTAATTGTGGACCGGGACATGGAATAAGCGTAGGAAGTCTTGGAAAGTATCTTGATGAAAAACCGGTATATGGCCTCATCATTAAGAACTGCACCCTCACGAACACAACAAACGGTGTGAGAATCAAGACATGGCCTGGTTCTCCCAGTGCCGGTTTTGCCTCACACATATACTTTGAGGATATTATCATGAATAATGTGAAGAACCCCATCATTATAGACCAAAACTACTGCCCCGGGCATAGTTGCAAACAACAGGCTCAGTCGAACGTTAAGATTAGTAATGTCAGCTTCAAGAACATCAGAGGCTCATCTGCATCACAAGTTGCCTTCAATCTTGCATGTAGTGGGAGCTTACCATGTGAGAATGTAGAACTGATAGGCATTGATCTCGTATACAGTGGAGATGGCGATCGACCTATTACATCTCGATGCTCTAATAATCAGAATGACTGA

Protein Analysis

520

Amino Acids

56.11

Weight (kDa)

6.63

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 283 - 517 2.4e-73 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 380
AccI GTMKAC 2 cut(s) 742, 1503
AciI CCGC 4 cut(s) 43, 524, 612, 615
AclI AACGTT 2 cut(s) 924, 1377
AclWI GGATC 4 cut(s) 389, 414, 545, 1031
AcoI YGGCCR 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 2 cut(s) 371, 533
AfiI CCNNNNNNNGG 7 cut(s) 52, 85, 684, 825, 1127, 1178, 1259
AgeI ACCGGT 1 cut(s) 1171
AgsI TTSAA 3 cut(s) 428, 1402, 1441
AjnI CCWGG 2 cut(s) 677, 1243
AluBI AGCT 4 cut(s) 312, 771, 1398, 1461
AluI AGCT 4 cut(s) 312, 771, 1398, 1461
Alw21I GWGCWC 3 cut(s) 314, 345, 498
Alw26I GTCTC 1 cut(s) 140
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 4 cut(s) 389, 414, 545, 1031
Ama87I CYCGRG 1 cut(s) 1343
AoxI GGCC 6 cut(s) 87, 686, 722, 764, 1180, 1241
ApaI GGGCCC 1 cut(s) 726
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 597, 620
AsiGI ACCGGT 1 cut(s) 1171
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 3 cut(s) 58, 602, 620
AspS9I GGNCC 4 cut(s) 687, 722, 723, 1118
AsuC2I CCSGG 6 cut(s) 66, 347, 975, 1122, 1344, 1345
AsuHPI GGTGA 4 cut(s) 272, 361, 403, 1088
AvaI CYCGRG 1 cut(s) 1343
AvaII GGWCC 1 cut(s) 1118
BaeGI GKGCMC 2 cut(s) 345, 726
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BalI TGGCCA 1 cut(s) 89
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 4 cut(s) 314, 726, 735, 820
Bbv12I GWGCWC 3 cut(s) 314, 345, 498
BbvI GCAGC 2 cut(s) 584, 607
BccI CCATC 8 cut(s) 473, 521, 554, 989, 1073, 1079, 1325, 1508
BcgI CGANNNNNNTGC 2 cut(s) 1480, 1514
BciT130I CCWGG 2 cut(s) 679, 1245
BciVI GTATCC 1 cut(s) 1030
BcnI CCSGG 6 cut(s) 66, 347, 975, 1122, 1344, 1345
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 3 cut(s) 431, 768, 785
BfoI RGCGCY 2 cut(s) 59, 621
BfuI GTATCC 1 cut(s) 1030
BglI GCCNNNNNGGC 1 cut(s) 730
BglII AGATCT 2 cut(s) 205, 463
BisI GCNGC 4 cut(s) 598, 613, 616, 621
BlsI GCNGC 4 cut(s) 599, 614, 617, 622
Bme1390I CCNGG 8 cut(s) 66, 347, 679, 975, 1122, 1245, 1344, 1345
Bme18I GGWCC 1 cut(s) 1118
BmeT110I CYCGRG 1 cut(s) 1343
BmgT120I GGNCC 4 cut(s) 687, 722, 723, 1118
BmiI GGNNCC 4 cut(s) 382, 724, 817, 1051
BmrFI CCNGG 8 cut(s) 66, 347, 679, 975, 1122, 1245, 1344, 1345
BmrI ACTGGG 1 cut(s) 1248
BmsI GCATC 5 cut(s) 253, 255, 342, 1433, 1529
BmuI ACTGGG 1 cut(s) 1248
BplI GAGNNNNNCTC 2 cut(s) 301, 333
Bpu10I CCTNAGC 1 cut(s) 273
BpuMI CCSGG 6 cut(s) 66, 347, 975, 1122, 1344, 1345
Bsa29I ATCGAT 1 cut(s) 549
BsaBI GATNNNNATC 3 cut(s) 267, 462, 1518
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 5 cut(s) 50, 305, 820, 1342, 1343
BsaWI WCCGGW 4 cut(s) 257, 573, 1052, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 7 cut(s) 52, 85, 684, 825, 1127, 1178, 1259
Bse118I RCCGGY 2 cut(s) 1171, 1259
Bse1I ACTGG 1 cut(s) 1254
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 3 cut(s) 267, 462, 1518
BseBI CCWGG 2 cut(s) 679, 1245
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 5 cut(s) 50, 305, 820, 1342, 1343
BseGI GGATG 2 cut(s) 532, 586
BseJI GATNNNNATC 3 cut(s) 267, 462, 1518
BseLI CCNNNNNNNGG 7 cut(s) 52, 85, 684, 825, 1127, 1178, 1259
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 2 cut(s) 357, 1382
BseNI ACTGG 1 cut(s) 1254
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 2 cut(s) 345, 726
BseXI GCAGC 2 cut(s) 584, 607
BsgI GTGCAG 1 cut(s) 1183
Bsh1285I CGRYCG 1 cut(s) 1522
BshFI GGCC 6 cut(s) 89, 688, 724, 766, 1182, 1243
BshNI GGYRCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 1171
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 1522
BsiHKAI GWGCWC 3 cut(s) 314, 345, 498
BsiHKCI CYCGRG 1 cut(s) 1343
BslFI GGGAC 3 cut(s) 217, 372, 1137
BslI CCNNNNNNNGG 7 cut(s) 52, 85, 684, 825, 1127, 1178, 1259
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 3 cut(s) 217, 372, 1137
BsnI GGCC 6 cut(s) 89, 688, 724, 766, 1182, 1243
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 1343
Bsp120I GGGCCC 1 cut(s) 722
Bsp1286I GDGCHC 6 cut(s) 314, 345, 498, 726, 735, 820
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 4 cut(s) 43, 524, 612, 615
BspANI GGCC 6 cut(s) 89, 688, 724, 766, 1182, 1243
BspCNI CTCAG 2 cut(s) 358, 1381
BspDI ATCGAT 1 cut(s) 549
BspHI TCATGA 1 cut(s) 1296
BspLI GGNNCC 4 cut(s) 382, 724, 817, 1051
BspPI GGATC 4 cut(s) 389, 414, 545, 1031
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 1005
BsrFI RCCGGY 2 cut(s) 1171, 1259
BsrI ACTGG 1 cut(s) 1254
BssAI RCCGGY 2 cut(s) 1171, 1259
BssECI CCNNGG 5 cut(s) 50, 305, 820, 1342, 1343
BssNAI GTATAC 1 cut(s) 1504
BssT1I CCWWGG 2 cut(s) 50, 820
Bst1107I GTATAC 1 cut(s) 1504
Bst2UI CCWGG 2 cut(s) 679, 1245
Bst4CI ACNGT 6 cut(s) 25, 649, 668, 964, 1223, 1508
BstC8I GCNNGC 2 cut(s) 675, 934
BstDEI CTNAG 3 cut(s) 273, 366, 1368
BstDSI CCRYGG 1 cut(s) 50
BstENI CCTNNNNNAGG 1 cut(s) 682
BstF5I GGATG 2 cut(s) 532, 586
BstH2I RGCGCY 2 cut(s) 59, 621
BstHHI GCGC 3 cut(s) 58, 602, 620
BstMAI GTCTC 1 cut(s) 140
BstMCI CGRYCG 1 cut(s) 1522
BstMWI GCNNNNNNNGC 2 cut(s) 730, 1265
BstNI CCWGG 2 cut(s) 679, 1245
BstNSI RCATGY 1 cut(s) 1452
BstSCI CCNGG 8 cut(s) 64, 345, 677, 973, 1120, 1243, 1342, 1343
BstSLI GKGCMC 2 cut(s) 345, 726
BstV1I GCAGC 2 cut(s) 584, 607
BstX2I RGATCY 3 cut(s) 205, 406, 463
BstXI CCANNNNNNTGG 1 cut(s) 668
BstYI RGATCY 3 cut(s) 205, 406, 463
BstZ17I GTATAC 1 cut(s) 1504
Bsu15I ATCGAT 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1030
BsuRI GGCC 6 cut(s) 89, 688, 724, 766, 1182, 1243
BsuTUI ATCGAT 1 cut(s) 549
BtgI CCRYGG 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 532, 586
BtsIMutI CAGTG 2 cut(s) 1261, 1513
Cac8I GCNNGC 2 cut(s) 675, 934
CciI TCATGA 1 cut(s) 1296
CfoI GCGC 3 cut(s) 58, 602, 620
Cfr10I RCCGGY 2 cut(s) 1171, 1259
Cfr13I GGNCC 4 cut(s) 687, 722, 723, 1118
Cfr9I CCCGGG 1 cut(s) 1343
ClaI ATCGAT 1 cut(s) 549
Csp6I GTAC 2 cut(s) 370, 532
CspAI ACCGGT 1 cut(s) 1171
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviQI GTAC 2 cut(s) 370, 532
DdeI CTNAG 3 cut(s) 273, 366, 1368
DraIII CACNNNGTG 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 87
EciI GGCGGA 1 cut(s) 539
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 50, 820
Eco147I AGGCCT 1 cut(s) 766
Eco24I GRGCYC 4 cut(s) 314, 726, 735, 820
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 1118
Eco53kI GAGCTC 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 1343
EcoICRI GAGCTC 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 723
EcoRII CCWGG 2 cut(s) 677, 1243
EcoT14I CCWWGG 2 cut(s) 50, 820
EcoT38I GRGCYC 4 cut(s) 314, 726, 735, 820
ErhI CCWWGG 2 cut(s) 50, 820
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 804, 836
FaqI GGGAC 3 cut(s) 217, 372, 1137
FauI CCCGC 1 cut(s) 50
FblI GTMKAC 2 cut(s) 742, 1503
Fnu4HI GCNGC 4 cut(s) 598, 613, 616, 621
FokI GGATG 2 cut(s) 539, 593
FriOI GRGCYC 4 cut(s) 314, 726, 735, 820
Fsp4HI GCNGC 4 cut(s) 598, 613, 616, 621
FspBI CTAG 3 cut(s) 431, 768, 785
GlaI GCGC 3 cut(s) 57, 601, 619
GluI GCNGC 4 cut(s) 598, 613, 616, 621
HaeII RGCGCY 2 cut(s) 59, 621
HaeIII GGCC 6 cut(s) 89, 688, 724, 766, 1182, 1243
HhaI GCGC 3 cut(s) 58, 602, 620
Hin6I GCGC 3 cut(s) 56, 600, 618
HinP1I GCGC 3 cut(s) 56, 600, 618
HinfI GANTC 3 cut(s) 540, 999, 1230
HphI GGTGA 4 cut(s) 272, 361, 403, 1088
Hpy166II GTNNAC 4 cut(s) 343, 743, 1118, 1504
Hpy188I TCNGA 7 cut(s) 80, 309, 394, 539, 1049, 1412, 1554
Hpy8I GTNNAC 4 cut(s) 343, 743, 1118, 1504
HpyAV CCTTC 3 cut(s) 117, 756, 1447
HpyCH4III ACNGT 6 cut(s) 25, 649, 668, 964, 1223, 1508
HpyCH4IV ACGT 5 cut(s) 849, 924, 1012, 1029, 1377
HpyCH4V TGCA 7 cut(s) 246, 343, 1010, 1200, 1356, 1424, 1448
HpyF10VI GCNNNNNNNGC 2 cut(s) 730, 1265
HpyF3I CTNAG 3 cut(s) 273, 366, 1368
HpySE526I ACGT 5 cut(s) 849, 924, 1012, 1029, 1377
HspAI GCGC 3 cut(s) 56, 600, 618
LmnI GCTCC 4 cut(s) 307, 309, 815, 1458
Lsp1109I GCAGC 2 cut(s) 584, 607
LweI GCATC 5 cut(s) 253, 255, 342, 1433, 1529
MaeI CTAG 3 cut(s) 431, 768, 785
MaeII ACGT 5 cut(s) 849, 924, 1012, 1029, 1377
MaeIII GTNAC 5 cut(s) 911, 943, 958, 1030, 1099
MboII GAAGA 5 cut(s) 458, 473, 621, 624, 1321
MflI RGATCY 3 cut(s) 205, 406, 463
MhlI GDGCHC 6 cut(s) 314, 345, 498, 726, 735, 820
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 2 cut(s) 879, 1111
MluNI TGGCCA 1 cut(s) 89
MmeI TCCRAC 4 cut(s) 454, 558, 806, 894
Mox20I TGGCCA 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 465
MscI TGGCCA 1 cut(s) 89
MseI TTAA 7 cut(s) 846, 900, 921, 987, 1110, 1191, 1380
MslI CAYNNNNRTG 2 cut(s) 1015, 1537
Msp20I TGGCCA 1 cut(s) 89
MspR9I CCNGG 8 cut(s) 66, 347, 679, 975, 1122, 1245, 1344, 1345
MvaI CCWGG 2 cut(s) 679, 1245
MwoI GCNNNNNNNGC 2 cut(s) 730, 1265
NciI CCSGG 6 cut(s) 66, 347, 975, 1122, 1344, 1345
NcoI CCATGG 1 cut(s) 50
NlaIV GGNNCC 4 cut(s) 382, 724, 817, 1051
NmuCI GTSAC 1 cut(s) 958
NspI RCATGY 1 cut(s) 1452
PagI TCATGA 1 cut(s) 1296
PceI AGGCCT 1 cut(s) 766
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 540, 999, 1230
PinAI ACCGGT 1 cut(s) 1171
PkrI GCNGC 4 cut(s) 599, 614, 617, 622
Ple19I CGATCG 1 cut(s) 1522
Psp124BI GAGCTC 1 cut(s) 314
Psp1406I AACGTT 2 cut(s) 924, 1377
Psp6I CCWGG 2 cut(s) 677, 1243
PspGI CCWGG 2 cut(s) 677, 1243
PspN4I GGNNCC 4 cut(s) 382, 724, 817, 1051
PspOMI GGGCCC 1 cut(s) 722
PspPI GGNCC 4 cut(s) 687, 722, 723, 1118
PsuI RGATCY 3 cut(s) 205, 406, 463
PvuI CGATCG 1 cut(s) 1522
RsaI GTAC 2 cut(s) 371, 533
RsaNI GTAC 2 cut(s) 370, 532
RseI CAYNNNNRTG 2 cut(s) 1015, 1537
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 7 cut(s) 846, 900, 921, 987, 1110, 1191, 1380
SatI GCNGC 4 cut(s) 598, 613, 616, 621
Sau96I GGNCC 4 cut(s) 687, 722, 723, 1118
ScrFI CCNGG 8 cut(s) 66, 347, 679, 975, 1122, 1245, 1344, 1345
SduI GDGCHC 6 cut(s) 314, 345, 498, 726, 735, 820
SfaNI GCATC 5 cut(s) 253, 255, 342, 1433, 1529
SinI GGWCC 1 cut(s) 1118
SmaI CCCGGG 1 cut(s) 1345
SmiMI CAYNNNNRTG 2 cut(s) 1015, 1537
Sse9I AATT 2 cut(s) 879, 1111
SseBI AGGCCT 1 cut(s) 766
SsiI CCGC 4 cut(s) 43, 524, 612, 615
SspI AATATT 1 cut(s) 197
SspMI CTAG 3 cut(s) 431, 768, 785
SstI GAGCTC 1 cut(s) 314
StuI AGGCCT 1 cut(s) 766
StyD4I CCNGG 8 cut(s) 64, 345, 677, 973, 1120, 1243, 1342, 1343
StyI CCWWGG 2 cut(s) 50, 820
TaaI ACNGT 6 cut(s) 25, 649, 668, 964, 1223, 1508
TaiI ACGT 5 cut(s) 852, 927, 1015, 1032, 1380
TaqI TCGA 5 cut(s) 549, 858, 1373, 1522, 1537
TasI AATT 2 cut(s) 879, 1111
TauI GCSGC 2 cut(s) 615, 618
TfiI GAWTC 3 cut(s) 540, 999, 1230
Tru1I TTAA 7 cut(s) 846, 900, 921, 987, 1110, 1191, 1380
Tru9I TTAA 7 cut(s) 846, 900, 921, 987, 1110, 1191, 1380
TscAI CASTG 2 cut(s) 1261, 1513
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 2 cut(s) 597, 620
Tsp45I GTSAC 1 cut(s) 958
TspDTI ATGAA 4 cut(s) 474, 1005, 1179, 1313
TspGWI ACGGA 1 cut(s) 1049
TspMI CCCGGG 1 cut(s) 1343
TspRI CASTG 2 cut(s) 1261, 1513
VneI GTGCAC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 1118
XagI CCTNNNNNAGG 1 cut(s) 682
XceI RCATGY 1 cut(s) 1452
XmaI CCCGGG 1 cut(s) 1343
XmiI GTMKAC 2 cut(s) 742, 1503
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 3 cut(s) 431, 768, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.