Rroxscaffold_2G00083870
WRKY Family

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007567
Physical Location & Seq
Reverse (-)
6442705 .. 6444889
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083870.1

Sequence Viewer

Length: 1215 bp
ATGGGTTGCATCAGCTCGAAGCAGGCGGCTCCGGGGTCTCCATGTATTGAGTATTCAGCGGCCCTGACCAAGAATGGCTCTGTTGTTCTCGAGCCTTCCGTGAGCAACAAGAAAAGCAAGAATACTACTCATCTTAATGCAATCCTCATTGTTCCAGTGCGAAACAACGGTAGTAGTAAGAGAAGCAGCAATAGTTTTAACAAGAAGGATCAGACTGATCAGAATCAGAATGTTCAGGAGAAGGACAAGGACAAGGAGAAGCCGTCCGACGATCACAAGAACAAGGAGCTGAAGAAGTCAAGAAAGGAGGGTCGCCGCCGGCGCTGCTTGGCTCAGCGCCGCTGCCAGGCCATCTACGGTTGGGTGCCCCTCAAAGCTGACGCTTACGAAAAATTGGAGAAGAGTATTAGGTTTATGGCTCGCGAGATTATGATTCTTCGTAGGCTTGATCATCCTAACATCATGAAGCTGGAGGGGATAATAACATCTCGATCATCGAGTAGCATATACCTAGTGTTTGAGTACATGGAGCATGATCTGGCTGGACTAGTAGCTGCAAATGACATCAAATTCAGTGACGTGCAGTTGGCAGATTTTGGATTGGCAAATGTGGTAAACGCAAAAAGCAATCAACCACTAACAAAGTCGGGTGGTGACCTTGTGGTATCGTCCTCCCGAGCTCTTGATGGGGGCAACAGAGTATGGAGTATCTGTGGATCTCTGGAGTGTGGGCTGTGTGTTTGCAGAACTCTATCTAGGGGAAGCCTGTTCTCAAAGGAAGAACTGAGGTTGAACAATTGCACAAAATTTTCAAGCTTTGTGGTTCTCCACCTGAAGATTACTGGAAGAACTCAAAGCCACCACGCAACAATGTTTAAACCTCAGTATTCTTATGAAAGTTCTCTTAGGGAAAGATGTAAGGAATTTCCAACCACATCGGTGGACTTGTTACAAACACTTCTTTCAGTAGAACTAACTACAACCGCGGAACTGCCTCCTCTGCCCTTATGTCTGAGAACAAAGCCTTACGCTTGCGATCCAGCAAAAGTGCCGAAGTATCCTCCCACCAAGGAAATGGATGCTAAAAGTCGTGAGGATGCACAGAAGAAAAAGCATGGTCCTCGAGCACGAGAGACAGCGGCCAAGAAACCAAGGAGATTCCGAAAGACTTTGCAAGAGACCAACTCTGCAAAGTTAGCACCAAAAAGAGGATAG

Protein Analysis

404

Amino Acids

45.23

Weight (kDa)

9.76

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 134 - 185 2.3e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 2 cut(s) 423, 986
AciI CCGC 7 cut(s) 26, 59, 316, 340, 984, 986, 1139
AclWI GGATC 3 cut(s) 216, 724, 1031
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 3 cut(s) 569, 806, 923
AcuI CTGAAG 2 cut(s) 311, 854
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 2 cut(s) 346, 1208
AgsI TTSAA 2 cut(s) 793, 813
AhlI ACTAGT 1 cut(s) 547
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 1 cut(s) 345
AleI CACNNNNGTG 1 cut(s) 938
AluBI AGCT 7 cut(s) 15, 289, 377, 469, 554, 680, 816
AluI AGCT 7 cut(s) 15, 289, 377, 469, 554, 680, 816
Alw21I GWGCWC 2 cut(s) 682, 1129
Alw26I GTCTC 3 cut(s) 42, 1127, 1172
AlwI GGATC 3 cut(s) 216, 724, 1031
Ama87I CYCGRG 3 cut(s) 89, 675, 1122
AoxI GGCC 3 cut(s) 60, 348, 1140
ApeKI GCWGC 4 cut(s) 186, 324, 342, 554
ApoI RAATTY 3 cut(s) 569, 806, 923
AspLEI GCGC 2 cut(s) 324, 339
AspS9I GGNCC 2 cut(s) 61, 1118
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 665
AvaI CYCGRG 3 cut(s) 89, 675, 1122
AvaII GGWCC 1 cut(s) 1118
BaeGI GKGCMC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 682
BauI CACGAG 1 cut(s) 1128
Bbv12I GWGCWC 2 cut(s) 682, 1129
BbvI GCAGC 4 cut(s) 198, 311, 329, 541
BccI CCATC 2 cut(s) 359, 680
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 1 cut(s) 347
BciVI GTATCC 1 cut(s) 1068
BclI TGATCA 2 cut(s) 217, 448
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 3 cut(s) 42, 1127, 1172
BcuI ACTAGT 1 cut(s) 547
BfaI CTAG 3 cut(s) 512, 548, 756
BfoI RGCGCY 2 cut(s) 325, 340
BfuI GTATCC 1 cut(s) 1068
BisI GCNGC 9 cut(s) 27, 60, 187, 316, 325, 340, 343, 555, 1140
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 9 cut(s) 28, 61, 188, 317, 326, 341, 344, 556, 1141
Bme1390I CCNGG 2 cut(s) 33, 347
Bme18I GGWCC 1 cut(s) 1118
BmeT110I CYCGRG 3 cut(s) 89, 675, 1122
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 61, 1118
BmiI GGNNCC 2 cut(s) 30, 366
BmrFI CCNGG 2 cut(s) 33, 347
BmsI GCATC 3 cut(s) 18, 1069, 1087
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 2 cut(s) 491, 743
Bpu1102I GCTNAGC 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 222
BsaI GGTCTC 2 cut(s) 42, 1172
BsaJI CCNNGG 4 cut(s) 32, 984, 1068, 1151
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 697, 727
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 1208
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 2 cut(s) 155, 847
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 4 cut(s) 32, 984, 1068, 1151
BseGI GGATG 3 cut(s) 451, 1084, 1102
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 346, 1208
BseMII CTCAG 4 cut(s) 347, 776, 896, 1004
BseNI ACTGG 2 cut(s) 155, 847
BseRI GAGGAG 1 cut(s) 987
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 4 cut(s) 198, 311, 329, 541
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 2 cut(s) 423, 986
BshFI GGCC 3 cut(s) 62, 350, 1142
BshNI GGYRCC 1 cut(s) 364
BsiHKAI GWGCWC 2 cut(s) 682, 1129
BsiHKCI CYCGRG 3 cut(s) 89, 675, 1122
BsiSI CCGG 2 cut(s) 32, 319
BslI CCNNNNNNNGG 2 cut(s) 346, 1208
BsmAI GTCTC 3 cut(s) 42, 1127, 1172
BsnI GGCC 3 cut(s) 62, 350, 1142
Bso31I GGTCTC 2 cut(s) 42, 1172
BsoBI CYCGRG 3 cut(s) 89, 675, 1122
Bsp1286I GDGCHC 3 cut(s) 369, 682, 1129
Bsp143I GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
Bsp1720I GCTNAGC 1 cut(s) 333
Bsp68I TCGCGA 1 cut(s) 423
BspACI CCGC 7 cut(s) 26, 59, 316, 340, 984, 986, 1139
BspANI GGCC 3 cut(s) 62, 350, 1142
BspCNI CTCAG 4 cut(s) 346, 777, 895, 1005
BspFNI CGCG 2 cut(s) 423, 986
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 30, 366
BspPI GGATC 3 cut(s) 216, 724, 1031
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 2 cut(s) 42, 1172
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 2 cut(s) 155, 847
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 4 cut(s) 32, 984, 1068, 1151
BssMI GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
BssSI CACGAG 1 cut(s) 1128
BssT1I CCWWGG 2 cut(s) 1068, 1151
Bst2BI CACGAG 1 cut(s) 1128
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 170, 359
Bst6I CTCTTC 1 cut(s) 395
BstC8I GCNNGC 4 cut(s) 24, 320, 421, 1033
BstDEI CTNAG 5 cut(s) 333, 785, 882, 905, 1013
BstDSI CCRYGG 1 cut(s) 984
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 3 cut(s) 451, 1084, 1102
BstFNI CGCG 2 cut(s) 423, 986
BstH2I RGCGCY 2 cut(s) 325, 340
BstHHI GCGC 2 cut(s) 324, 339
BstKTI GATC 8 cut(s) 211, 220, 274, 451, 494, 538, 719, 1039
BstMAI GTCTC 3 cut(s) 42, 1127, 1172
BstMBI GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
BstMWI GCNNNNNNNGC 4 cut(s) 321, 324, 1000, 1196
BstNI CCWGG 1 cut(s) 347
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 31, 345
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 2 cut(s) 423, 986
BstV1I GCAGC 4 cut(s) 198, 311, 329, 541
BstX2I RGATCY 1 cut(s) 716
BstXI CCANNNNNNTGG 2 cut(s) 940, 1075
BstYI RGATCY 1 cut(s) 716
BsuI GTATCC 1 cut(s) 1068
BsuRI GGCC 3 cut(s) 62, 350, 1142
BtgI CCRYGG 1 cut(s) 984
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 3 cut(s) 451, 1084, 1102
BtsIMutI CAGTG 2 cut(s) 162, 580
BtuMI TCGCGA 1 cut(s) 423
Cac8I GCNNGC 4 cut(s) 24, 320, 421, 1033
CciI TCATGA 1 cut(s) 462
CfoI GCGC 2 cut(s) 324, 339
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 2 cut(s) 61, 1118
Cfr42I CCGCGG 1 cut(s) 987
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 523
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 5 cut(s) 42, 463, 526, 533, 1115
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 5 cut(s) 333, 785, 882, 905, 1013
DpnI GATC 8 cut(s) 210, 219, 273, 450, 493, 537, 718, 1038
DpnII GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
DraI TTTAAA 1 cut(s) 877
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 680
Eco130I CCWWGG 2 cut(s) 1068, 1151
Eco24I GRGCYC 1 cut(s) 682
Eco31I GGTCTC 2 cut(s) 42, 1172
Eco47I GGWCC 1 cut(s) 1118
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 2 cut(s) 311, 854
Eco88I CYCGRG 3 cut(s) 89, 675, 1122
Eco91I GGTNACC 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 680
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 1 cut(s) 345
EcoT14I CCWWGG 2 cut(s) 1068, 1151
EcoT38I GRGCYC 1 cut(s) 682
ErhI CCWWGG 2 cut(s) 1068, 1151
FaeI CATG 5 cut(s) 45, 466, 529, 536, 1118
FatI CATG 5 cut(s) 41, 462, 525, 532, 1114
FbaI TGATCA 2 cut(s) 217, 448
Fnu4HI GCNGC 9 cut(s) 27, 60, 187, 316, 325, 340, 343, 555, 1140
FokI GGATG 3 cut(s) 438, 1091, 1109
FriOI GRGCYC 1 cut(s) 682
Fsp4HI GCNGC 9 cut(s) 27, 60, 187, 316, 325, 340, 343, 555, 1140
FspBI CTAG 3 cut(s) 512, 548, 756
GlaI GCGC 2 cut(s) 323, 338
GluI GCNGC 9 cut(s) 27, 60, 187, 316, 325, 340, 343, 555, 1140
GsuI CTGGAG 2 cut(s) 491, 743
HaeII RGCGCY 2 cut(s) 325, 340
HaeIII GGCC 3 cut(s) 62, 350, 1142
HapII CCGG 2 cut(s) 32, 319
HgaI GACGC 1 cut(s) 389
HhaI GCGC 2 cut(s) 324, 339
Hin1II CATG 5 cut(s) 45, 466, 529, 536, 1118
Hin6I GCGC 2 cut(s) 322, 337
HinP1I GCGC 2 cut(s) 322, 337
HindIII AAGCTT 1 cut(s) 814
HinfI GANTC 3 cut(s) 223, 433, 1158
HpaII CCGG 2 cut(s) 32, 319
HphI GGTGA 1 cut(s) 665
Hpy166II GTNNAC 2 cut(s) 616, 943
Hpy188I TCNGA 6 cut(s) 213, 222, 228, 268, 1014, 1163
Hpy8I GTNNAC 2 cut(s) 616, 943
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 3 cut(s) 105, 199, 235
HpyCH4III ACNGT 2 cut(s) 170, 359
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 9 cut(s) 9, 140, 557, 583, 744, 801, 1100, 1174, 1190
HpyF10VI GCNNNNNNNGC 4 cut(s) 321, 324, 1000, 1196
HpyF3I CTNAG 5 cut(s) 333, 785, 882, 905, 1013
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 5 cut(s) 45, 466, 529, 536, 1118
HspAI GCGC 2 cut(s) 322, 337
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 2 cut(s) 217, 448
KspI CCGCGG 1 cut(s) 987
Kzo9I GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
LmnI GCTCC 3 cut(s) 34, 286, 529
Lsp1109I GCAGC 4 cut(s) 198, 311, 329, 541
LweI GCATC 3 cut(s) 18, 1069, 1087
MaeI CTAG 3 cut(s) 512, 548, 756
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 3 cut(s) 575, 653, 948
MalI GATC 8 cut(s) 210, 219, 273, 450, 493, 537, 718, 1038
MboI GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
MboII GAAGA 7 cut(s) 304, 412, 428, 791, 847, 858, 1117
MfeI CAATTG 1 cut(s) 796
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 3 cut(s) 369, 682, 1129
MluCI AATT 5 cut(s) 392, 569, 796, 806, 923
MmeI TCCRAC 2 cut(s) 291, 953
MreI CGCCGGCG 1 cut(s) 318
MroNI GCCGGC 1 cut(s) 318
MseI TTAA 3 cut(s) 135, 198, 876
MslI CAYNNNNRTG 2 cut(s) 135, 938
MspA1I CMGCKG 4 cut(s) 59, 342, 986, 1139
MspI CCGG 2 cut(s) 32, 319
MspR9I CCNGG 2 cut(s) 33, 347
MssI GTTTAAAC 1 cut(s) 877
MunI CAATTG 1 cut(s) 796
MvaI CCWGG 1 cut(s) 347
MvnI CGCG 2 cut(s) 423, 986
MwoI GCNNNNNNNGC 4 cut(s) 321, 324, 1000, 1196
NaeI GCCGGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 33
NdeII GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
NgoMIV GCCGGC 1 cut(s) 318
NlaIII CATG 5 cut(s) 45, 466, 529, 536, 1118
NlaIV GGNNCC 2 cut(s) 30, 366
NmuCI GTSAC 2 cut(s) 575, 653
NruI TCGCGA 1 cut(s) 423
OliI CACNNNNGTG 1 cut(s) 938
PaeR7I CTCGAG 2 cut(s) 89, 1122
PagI TCATGA 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 320
PfeI GAWTC 3 cut(s) 223, 433, 1158
PkrI GCNGC 9 cut(s) 28, 61, 188, 317, 326, 341, 344, 556, 1141
PmeI GTTTAAAC 1 cut(s) 877
Psp124BI GAGCTC 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 345
PspEI GGTNACC 1 cut(s) 653
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 30, 366
PspPI GGNCC 2 cut(s) 61, 1118
PspXI VCTCGAGB 1 cut(s) 1122
PsuI RGATCY 1 cut(s) 716
RruI TCGCGA 1 cut(s) 423
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 2 cut(s) 135, 938
SacI GAGCTC 1 cut(s) 682
SacII CCGCGG 1 cut(s) 987
SaqAI TTAA 3 cut(s) 135, 198, 876
SatI GCNGC 9 cut(s) 27, 60, 187, 316, 325, 340, 343, 555, 1140
Sau3AI GATC 8 cut(s) 208, 217, 271, 448, 491, 535, 716, 1036
Sau96I GGNCC 2 cut(s) 61, 1118
ScrFI CCNGG 2 cut(s) 33, 347
SduI GDGCHC 3 cut(s) 369, 682, 1129
SfaNI GCATC 3 cut(s) 18, 1069, 1087
Sfr274I CTCGAG 2 cut(s) 89, 1122
Sfr303I CCGCGG 1 cut(s) 987
SgrAI CRCCGGYG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 1 cut(s) 1118
SlaI CTCGAG 2 cut(s) 89, 1122
SmiMI CAYNNNNRTG 2 cut(s) 135, 938
SmlI CTYRAG 2 cut(s) 89, 1122
SmoI CTYRAG 2 cut(s) 89, 1122
SpeI ACTAGT 1 cut(s) 547
Sse9I AATT 5 cut(s) 392, 569, 796, 806, 923
SsiI CCGC 7 cut(s) 26, 59, 316, 340, 984, 986, 1139
SspMI CTAG 3 cut(s) 512, 548, 756
SstI GAGCTC 1 cut(s) 682
StyD4I CCNGG 2 cut(s) 31, 345
StyI CCWWGG 2 cut(s) 1068, 1151
TaaI ACNGT 2 cut(s) 170, 359
TaiI ACGT 1 cut(s) 582
TaqI TCGA 5 cut(s) 17, 90, 490, 497, 1123
TasI AATT 5 cut(s) 392, 569, 796, 806, 923
TatI WGTACW 1 cut(s) 522
TauI GCSGC 5 cut(s) 29, 62, 318, 342, 1142
TfiI GAWTC 3 cut(s) 223, 433, 1158
Tru1I TTAA 3 cut(s) 135, 198, 876
Tru9I TTAA 3 cut(s) 135, 198, 876
TscAI CASTG 2 cut(s) 162, 580
TseFI GTSAC 2 cut(s) 575, 653
TseI GCWGC 4 cut(s) 186, 324, 342, 554
Tsp45I GTSAC 2 cut(s) 575, 653
TspDTI ATGAA 2 cut(s) 479, 909
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 2 cut(s) 162, 580
VpaK11BI GGWCC 1 cut(s) 1118
XapI RAATTY 3 cut(s) 569, 806, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XhoI CTCGAG 2 cut(s) 89, 1122
XspI CTAG 3 cut(s) 512, 548, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.