MD09G1074500.v1.1

HAUS augmin-like complex subunit 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010982
Physical Location & Seq
Forward (+)
5177188 .. 5183842
6655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1074500.v1.1.491

Sequence Viewer

Length: 2379 bp
ATGCAGGGCTCGTCGCAGAGCTCTGCGGCGGCTCAGCCGGAGGCGATATTGCAATGGCTTCAGAAGGAAATGGGGTACCGGCCTTTGGGTCCGTACAGTGCTGCTTCTAGCAAATCGCAATTGCCCTCAATCGACTCGATGAGGAAGATTTGCCGTGGGAACATGATACCCATTTGGAATTTCTTGATTACCCGCGTGAAGTCGGAGAACACGGTGAAGAATATTCGGAGGAATATAACGGTTCACGGTGGTGGCGGTGGCGGGGATGGTGGCGCATTGGTAAAATCGAGGAAGGAGGAGGGTACCAGGAGTAAAGGAGGTAGGAGAAAGGAGAAGCTTGGGGAGGGTACGAGTGCGGCGGAGACTAGGGAGGCCGCGCTGCAAGAGAGGGACTTGGCGGCAAAGGAGGTGGAGAAGTTGAGGAACATTGTGAAGAGGCAGAGGAAGGATTTGAAGGCCAGAATGTTGGAGGTTTCAAGGGCCGAGGCCGAACGGAAGAGGATGCTTGATGAACGCTCTAAAAAAAGGCATAAGCAAGTAATGTTGGAGGCTTATTATCAACAGTGTGATGAAGCGGAAAAGATATTTGCAGAGTATCATAAACGTCTTCGTTATTATGTTAATCAAGCAAGAGATGCTCAAAGGTCTGGTGTTGATTCATCTCTTGAATTGGTTAACAGTTTTGGTTCAAATAGTGAAAAGGAAGCTGTTTATTCAACTCTTAAGGGCAGTAAAGCTGCAGAAGATGTACTTCTTATTGAAACCACTAAGGAAAGAAATATCCGAAATGCCTGTGAATCTCTTGCAGCACATATGATAGAAAAAATTCGATGTTCTTTTCCTGCATATGAAGGAAGTGGTATTCATTTGAATCCTCAGTTAGAAGCAGCCAAGTTAGGCTTTAATTTTGATGGGGAATTACCCGATGAGGTTAGAGCTGCAATTGTGAATGGGTTGAAGAATCCTCCTCAATTGCTTGAGGCAATTACTTCATACACATCACGGTTGAAATCTCTAATTTCCAGAGAGATAGAGAAAATTGATGTTAGAGCTGATGCAGAAACTTTGAGGTACAAGTATGAGAACAACCGAGTTATGGATGTTTCTTCTCCTGATGTGAGTTCACCATTACACTATCAACTATATGGTAATGGAAAGATAAGAGCGGATGCACCTTCTAGAGGAACTCAACTTCTTGAAAGACAGAATGCACATGTTCAGCAATTTTTGGCTACTGAAGATGCACTGAACAAAGCTGCAGAAGCTAGGGACCTATGTCAAAAACTTATAAAACGCTTGCATGGCAATAGTGATGTAATTTCTTCAGGAACATCACAAAATGTGGGCAGTCTTAGGCAACTTGAGCTGGAGGTTTGGGCCAAGGAAAGAGAAGTTGCTGGCTTGAGGGCTAGCTTGAATACACTGATGTCTGAAATACAACGCTTAAATAAATTATGTGCGGAAAGGAAAGAAGCTGAAGATTCTTTGAAAAAGAAGTGGAAGAAGATTGAAGAATTTGATTACCGCAGATCAGAACTTGAAATCATATATACCTCATTACTAAAAGTTAACATGGATGCTGCTGCCTTCTGGAATCAGCAGCCATTAGCCGCACGGGAGTATGCATCAAGCACTATTATTCCTGCATGCACAATAGTCATGGATCTTTCAAATGGTGCAAAAGATCTTATTGAAAGAGAAGTTTCTGCTTTTGACCAAAGTCCTGATAATAGCCTCTACATGCTTCCAGCAACTCCACAGGCACTATTGGAGTCCATGGGTACAAATGGGTCTACTGGACCTGAAGCTGTTGCTGCTGCAGAAAAAAATGCTGCTATATTAACTGCAAAAGCAGGAGCTAGGGATCCATCAGCAATACCATCCATATGTCGCATATCTGCGGCCCTTCAGTATCCTGGTGGTTTGGAGGGTTCAGACGCTGCCCTAGCATCAATCCTAGAGTCCCTGGAATTCTGTTTGAAACTTCGTGGTTCTGAAGCTAGTGTGTTGGAGGACTTGGTAAAGGCAATCAATTTGGTCCATACACGACAGGATCTTGTTGAAAGTGGTCATGTATTGTTGAACCATGCCTATAGAGCTCAACAAGAATATGAAAGGACAACGAGCTATTGTTTAAATATGGCTGCTGAGCAAGAGAAAATTGTCATGGAGAAATGGTTGCCTGAACTTAAGGCTGCAATTTTGAGTGCTCAGAAGTGCTTGGAAGATTGCAACTATGTTAGGGGATTGCTTGATGAATGGTGGGAGCAACCAGCAGCAACTGTTGTTGACTGGGTCCTAGTCGATGGATTAAACGTTGCTGCCTGGCATAATCATGTGAAGCAGCTCCTTGCATTTTATGATCAGGAGCACTTGTGA

Protein Analysis

793

Amino Acids

88.34

Weight (kDa)

7.21

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS5 PF14817 13 - 778 7.2e-249 HAUS augmin-like complex subunit 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1289
Acc65I GGTACC 2 cut(s) 75, 302
AccB1I GGYRCC 2 cut(s) 75, 302
AccBSI CCGCTC 1 cut(s) 1166
AccI GTMKAC 1 cut(s) 1793
AccII CGCG 2 cut(s) 195, 377
AclI AACGTT 1 cut(s) 2316
AclWI GGATC 4 cut(s) 1671, 1859, 1872, 2061
AcsI RAATTY 4 cut(s) 178, 825, 1514, 1970
AcuI CTGAAG 7 cut(s) 44, 1257, 1308, 1497, 1824, 1892, 2016
AfaI GTAC 7 cut(s) 77, 95, 304, 349, 750, 1073, 1783
AfiI CCNNNNNNNGG 3 cut(s) 85, 1096, 1181
AflII CTTAAG 2 cut(s) 722, 2189
AflIII ACRYGT 1 cut(s) 1213
AjnI CCWGG 4 cut(s) 305, 1915, 1965, 2324
AleI CACNNNNGTG 1 cut(s) 249
Alw21I GWGCWC 4 cut(s) 23, 2101, 2212, 2373
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 4 cut(s) 1671, 1859, 1872, 2061
AlwNI CAGNNNCTG 2 cut(s) 1940, 2282
AoxI GGCC 7 cut(s) 80, 372, 456, 480, 486, 1377, 1902
ApoI RAATTY 4 cut(s) 178, 825, 1514, 1970
Asp700I GAANNNNTTC 1 cut(s) 825
Asp718I GGTACC 2 cut(s) 75, 302
AspLEI GCGC 2 cut(s) 275, 379
AspS9I GGNCC 8 cut(s) 89, 480, 1270, 1377, 1799, 1903, 2038, 2296
AsuHPI GGTGA 2 cut(s) 226, 1116
AsuNHI GCTAGC 1 cut(s) 1409
AvaII GGWCC 5 cut(s) 89, 1270, 1799, 2038, 2296
BamHI GGATCC 1 cut(s) 1864
BanI GGYRCC 2 cut(s) 75, 302
BanII GRGCYC 3 cut(s) 11, 23, 2101
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 4 cut(s) 23, 2101, 2212, 2373
BccI CCATC 5 cut(s) 260, 905, 1876, 1888, 2300
BceAI ACGGC 1 cut(s) 138
BciT130I CCWGG 4 cut(s) 307, 1917, 1967, 2326
BciVI GTATCC 1 cut(s) 1923
BclI TGATCA 1 cut(s) 2362
BcoDI GTCTC 1 cut(s) 356
BfmI CTRYAG 4 cut(s) 738, 1257, 1818, 2092
BfrI CTTAAG 2 cut(s) 722, 2189
BfuI GTATCC 1 cut(s) 1923
BglII AGATCT 1 cut(s) 1684
BlpI GCTNAGC 2 cut(s) 33, 2148
Bme1390I CCNGG 4 cut(s) 307, 1917, 1967, 2326
Bme18I GGWCC 5 cut(s) 89, 1270, 1799, 2038, 2296
BmgT120I GGNCC 8 cut(s) 89, 480, 1270, 1377, 1799, 1903, 2038, 2296
BmiI GGNNCC 6 cut(s) 77, 90, 304, 1271, 1866, 2297
BmrFI CCNGG 4 cut(s) 307, 1917, 1967, 2326
BmrI ACTGGG 1 cut(s) 2302
BmsI GCATC 8 cut(s) 492, 625, 1045, 1159, 1231, 1567, 1634, 1958
BmtI GCTAGC 1 cut(s) 1413
BmuI ACTGGG 1 cut(s) 2302
BoxI GACNNNNGTC 2 cut(s) 1275, 1719
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 1388
Bpu1102I GCTNAGC 2 cut(s) 33, 2148
BpuEI CTTGAG 3 cut(s) 998, 1382, 1423
BsaJI CCNNGG 5 cut(s) 154, 483, 1380, 1776, 1965
BsaXI ACNNNNNCTCC 3 cut(s) 2162, 2192, 2360
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 1096, 1181
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 1801, 2297
Bse3DI GCAATG 1 cut(s) 59
BseBI CCWGG 4 cut(s) 307, 1917, 1967, 2326
BseDI CCNNGG 5 cut(s) 154, 483, 1380, 1776, 1965
BseGI GGATG 6 cut(s) 271, 507, 1105, 1174, 1582, 1880
BseLI CCNNNNNNNGG 3 cut(s) 85, 1096, 1181
BseMI GCAATG 1 cut(s) 59
BseMII CTCAG 4 cut(s) 47, 890, 2139, 2225
BseNI ACTGG 2 cut(s) 1801, 2297
BseRI GAGGAG 2 cut(s) 311, 957
Bsh1236I CGCG 2 cut(s) 195, 377
BshFI GGCC 7 cut(s) 82, 374, 458, 482, 488, 1379, 1904
BshNI GGYRCC 2 cut(s) 75, 302
BsiHKAI GWGCWC 4 cut(s) 23, 2101, 2212, 2373
BsiSI CCGG 2 cut(s) 38, 79
BslFI GGGAC 3 cut(s) 404, 1283, 1948
BslI CCNNNNNNNGG 3 cut(s) 85, 1096, 1181
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 3 cut(s) 404, 1283, 1948
BsmI GAATGC 1 cut(s) 1213
BsnI GGCC 7 cut(s) 82, 374, 458, 482, 488, 1379, 1904
Bsp1286I GDGCHC 5 cut(s) 11, 23, 2101, 2212, 2373
Bsp143I GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
Bsp1720I GCTNAGC 2 cut(s) 33, 2148
Bsp19I CCATGG 1 cut(s) 1776
BspANI GGCC 7 cut(s) 82, 374, 458, 482, 488, 1379, 1904
BspCNI CTCAG 4 cut(s) 46, 889, 2140, 2224
BspFNI CGCG 2 cut(s) 195, 377
BspLI GGNNCC 6 cut(s) 77, 90, 304, 1271, 1866, 2297
BspMAI CTGCAG 3 cut(s) 742, 1261, 1822
BspOI GCTAGC 1 cut(s) 1413
BspPI GGATC 4 cut(s) 1671, 1859, 1872, 2061
BspT107I GGYRCC 2 cut(s) 75, 302
BspTI CTTAAG 2 cut(s) 722, 2189
BsrBI CCGCTC 1 cut(s) 1166
BsrDI GCAATG 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 2 cut(s) 1801, 2297
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 5 cut(s) 154, 483, 1380, 1776, 1965
BssMI GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
BssT1I CCWWGG 2 cut(s) 1380, 1776
Bst2UI CCWGG 4 cut(s) 307, 1917, 1967, 2326
Bst4CI ACNGT 8 cut(s) 98, 214, 241, 248, 564, 680, 1005, 2284
Bst6I CTCTTC 2 cut(s) 428, 491
BstAFI CTTAAG 2 cut(s) 722, 2189
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 1298, 1399, 1411, 1648
BstDEI CTNAG 6 cut(s) 33, 768, 876, 1352, 2148, 2211
BstDSI CCRYGG 2 cut(s) 154, 1776
BstENI CCTNNNNNAGG 1 cut(s) 1179
BstF5I GGATG 6 cut(s) 271, 507, 1105, 1174, 1582, 1880
BstFNI CGCG 2 cut(s) 195, 377
BstHHI GCGC 2 cut(s) 275, 379
BstKTI GATC 6 cut(s) 1532, 1666, 1687, 1867, 2056, 2365
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
BstMWI GCNNNNNNNGC 7 cut(s) 635, 1262, 1302, 1363, 1814, 1946, 2096
BstNI CCWGG 4 cut(s) 307, 1917, 1967, 2326
BstNSI RCATGY 3 cut(s) 1217, 1650, 1744
BstPAI GACNNNNGTC 2 cut(s) 1275, 1719
BstSCI CCNGG 4 cut(s) 305, 1915, 1965, 2324
BstSFI CTRYAG 4 cut(s) 738, 1257, 1818, 2092
BstUI CGCG 2 cut(s) 195, 377
BstV2I GAAGAC 1 cut(s) 599
BstX2I RGATCY 4 cut(s) 1663, 1684, 1864, 2053
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 4 cut(s) 1663, 1684, 1864, 2053
BsuI GTATCC 1 cut(s) 1923
BsuRI GGCC 7 cut(s) 82, 374, 458, 482, 488, 1379, 1904
BtgI CCRYGG 2 cut(s) 154, 1776
BtsCI GGATG 6 cut(s) 271, 507, 1105, 1174, 1582, 1880
BtsIMutI CAGTG 4 cut(s) 103, 569, 1244, 1421
Cac8I GCNNGC 4 cut(s) 1298, 1399, 1411, 1648
CaiI CAGNNNCTG 2 cut(s) 1940, 2282
CfoI GCGC 2 cut(s) 275, 379
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 8 cut(s) 89, 480, 1270, 1377, 1799, 1903, 2038, 2296
CseI GACGC 1 cut(s) 1946
Csp6I GTAC 7 cut(s) 76, 94, 303, 348, 749, 1072, 1782
CspCI CAANNNNNGTGG 2 cut(s) 390, 425
CviQI GTAC 7 cut(s) 76, 94, 303, 348, 749, 1072, 1782
DdeI CTNAG 6 cut(s) 33, 768, 876, 1352, 2148, 2211
DpnI GATC 6 cut(s) 1531, 1665, 1686, 1866, 2055, 2364
DpnII GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
DraI TTTAAA 1 cut(s) 2136
Eam1104I CTCTTC 2 cut(s) 428, 491
EarI CTCTTC 2 cut(s) 428, 491
EciI GGCGGA 1 cut(s) 374
Ecl136II GAGCTC 2 cut(s) 21, 2099
Eco130I CCWWGG 2 cut(s) 1380, 1776
Eco24I GRGCYC 3 cut(s) 11, 23, 2101
Eco47I GGWCC 5 cut(s) 89, 1270, 1799, 2038, 2296
Eco53kI GAGCTC 2 cut(s) 21, 2099
Eco57I CTGAAG 7 cut(s) 44, 1257, 1308, 1497, 1824, 1892, 2016
EcoICRI GAGCTC 2 cut(s) 21, 2099
EcoNI CCTNNNNNAGG 1 cut(s) 1179
EcoO109I RGGNCCY 2 cut(s) 1270, 2296
EcoRI GAATTC 1 cut(s) 1970
EcoRII CCWGG 4 cut(s) 305, 1915, 1965, 2324
EcoT14I CCWWGG 2 cut(s) 1380, 1776
EcoT22I ATGCAT 1 cut(s) 1627
EcoT38I GRGCYC 3 cut(s) 11, 23, 2101
ErhI CCWWGG 2 cut(s) 1380, 1776
FalI AAGNNNNNCTT 4 cut(s) 735, 767, 884, 916
FaqI GGGAC 3 cut(s) 404, 1283, 1948
FauI CCCGC 2 cut(s) 200, 254
FauNDI CATATG 3 cut(s) 813, 847, 1886
FbaI TGATCA 1 cut(s) 2362
FblI GTMKAC 1 cut(s) 1793
FokI GGATG 6 cut(s) 278, 514, 1112, 1181, 1589, 1867
FriOI GRGCYC 3 cut(s) 11, 23, 2101
GlaI GCGC 2 cut(s) 274, 378
GsuI CTGGAG 1 cut(s) 1388
HaeIII GGCC 7 cut(s) 82, 374, 458, 482, 488, 1379, 1904
HapII CCGG 2 cut(s) 38, 79
HgaI GACGC 1 cut(s) 1946
HhaI GCGC 2 cut(s) 275, 379
Hin6I GCGC 2 cut(s) 273, 377
HinP1I GCGC 2 cut(s) 273, 377
HincII GTYRAC 3 cut(s) 676, 1570, 2290
HindII GTYRAC 3 cut(s) 676, 1570, 2290
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 9 cut(s) 134, 656, 797, 871, 961, 1481, 1594, 1772, 1961
HpaI GTTAAC 2 cut(s) 676, 1570
HpaII CCGG 2 cut(s) 38, 79
HphI GGTGA 2 cut(s) 226, 1116
Hpy166II GTNNAC 6 cut(s) 244, 676, 1124, 1570, 1794, 2290
Hpy188I TCNGA 9 cut(s) 63, 205, 228, 785, 1432, 1534, 1936, 1996, 2214
Hpy8I GTNNAC 6 cut(s) 244, 676, 1124, 1570, 1794, 2290
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 8 cut(s) 58, 286, 439, 448, 845, 1185, 1597, 1916
HpyCH4III ACNGT 8 cut(s) 98, 214, 241, 248, 564, 680, 1005, 2284
HpyCH4IV ACGT 2 cut(s) 604, 2316
HpyF10VI GCNNNNNNNGC 7 cut(s) 635, 1262, 1302, 1363, 1814, 1946, 2096
HpyF3I CTNAG 6 cut(s) 33, 768, 876, 1352, 2148, 2211
HpySE526I ACGT 2 cut(s) 604, 2316
HspAI GCGC 2 cut(s) 273, 377
KpnI GGTACC 2 cut(s) 79, 306
Ksp22I TGATCA 1 cut(s) 2362
KspAI GTTAAC 2 cut(s) 676, 1570
Kzo9I GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
LmnI GCTCC 4 cut(s) 1856, 2266, 2352, 2368
LweI GCATC 8 cut(s) 492, 625, 1045, 1159, 1231, 1567, 1634, 1958
MaeII ACGT 2 cut(s) 604, 2316
MalI GATC 6 cut(s) 1531, 1665, 1686, 1866, 2055, 2364
MbiI CCGCTC 1 cut(s) 1166
MboI GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
MfeI CAATTG 3 cut(s) 119, 942, 971
MflI RGATCY 4 cut(s) 1663, 1684, 1864, 2053
MhlI GDGCHC 5 cut(s) 11, 23, 2101, 2212, 2373
MlyI GAGTC 3 cut(s) 128, 1781, 1970
MmeI TCCRAC 4 cut(s) 183, 447, 525, 1989
Mph1103I ATGCAT 1 cut(s) 1627
MroXI GAANNNNTTC 1 cut(s) 825
MslI CAYNNNNRTG 3 cut(s) 249, 1885, 2334
MspCI CTTAAG 2 cut(s) 722, 2189
MspI CCGG 2 cut(s) 38, 79
MspR9I CCNGG 4 cut(s) 307, 1917, 1967, 2326
MunI CAATTG 3 cut(s) 119, 942, 971
Mva1269I GAATGC 1 cut(s) 1213
MvaI CCWGG 4 cut(s) 307, 1917, 1967, 2326
MvnI CGCG 2 cut(s) 195, 377
MwoI GCNNNNNNNGC 7 cut(s) 635, 1262, 1302, 1363, 1814, 1946, 2096
NcoI CCATGG 1 cut(s) 1776
NdeI CATATG 3 cut(s) 813, 847, 1886
NdeII GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
NheI GCTAGC 1 cut(s) 1409
NlaIV GGNNCC 6 cut(s) 77, 90, 304, 1271, 1866, 2297
NmeAIII GCCGAG 1 cut(s) 508
NsiI ATGCAT 1 cut(s) 1627
NspI RCATGY 3 cut(s) 1217, 1650, 1744
OliI CACNNNNGTG 1 cut(s) 249
PaeI GCATGC 1 cut(s) 1650
PciI ACATGT 1 cut(s) 1213
PctI GAATGC 1 cut(s) 1213
PdmI GAANNNNTTC 1 cut(s) 825
PfeI GAWTC 6 cut(s) 656, 797, 871, 961, 1481, 1594
PflFI GACNNNGTC 1 cut(s) 2294
PleI GAGTC 3 cut(s) 128, 1780, 1969
PpsI GAGTC 3 cut(s) 128, 1780, 1969
PpuMI RGGWCCY 2 cut(s) 1270, 2296
PscI ACATGT 1 cut(s) 1213
PshAI GACNNNNGTC 2 cut(s) 1275, 1719
PsiI TTATAA 1 cut(s) 1289
Psp124BI GAGCTC 2 cut(s) 23, 2101
Psp1406I AACGTT 1 cut(s) 2316
Psp5II RGGWCCY 2 cut(s) 1270, 2296
Psp6I CCWGG 4 cut(s) 305, 1915, 1965, 2324
PspGI CCWGG 4 cut(s) 305, 1915, 1965, 2324
PspN4I GGNNCC 6 cut(s) 77, 90, 304, 1271, 1866, 2297
PspPI GGNCC 8 cut(s) 89, 480, 1270, 1377, 1799, 1903, 2038, 2296
PspPPI RGGWCCY 2 cut(s) 1270, 2296
PstI CTGCAG 3 cut(s) 742, 1261, 1822
PstNI CAGNNNCTG 2 cut(s) 1940, 2282
PsuI RGATCY 4 cut(s) 1663, 1684, 1864, 2053
PsyI GACNNNGTC 1 cut(s) 2294
RsaI GTAC 7 cut(s) 77, 95, 304, 349, 750, 1073, 1783
RsaNI GTAC 7 cut(s) 76, 94, 303, 348, 749, 1072, 1782
RseI CAYNNNNRTG 3 cut(s) 249, 1885, 2334
SacI GAGCTC 2 cut(s) 23, 2101
Sau3AI GATC 6 cut(s) 1529, 1663, 1684, 1864, 2053, 2362
Sau96I GGNCC 8 cut(s) 89, 480, 1270, 1377, 1799, 1903, 2038, 2296
SchI GAGTC 3 cut(s) 128, 1781, 1970
ScrFI CCNGG 4 cut(s) 307, 1917, 1967, 2326
SduI GDGCHC 5 cut(s) 11, 23, 2101, 2212, 2373
SfaNI GCATC 8 cut(s) 492, 625, 1045, 1159, 1231, 1567, 1634, 1958
SfcI CTRYAG 4 cut(s) 738, 1257, 1818, 2092
SinI GGWCC 5 cut(s) 89, 1270, 1799, 2038, 2296
SmiMI CAYNNNNRTG 3 cut(s) 249, 1885, 2334
SmlI CTYRAG 5 cut(s) 722, 977, 1361, 1402, 2189
SmoI CTYRAG 5 cut(s) 722, 977, 1361, 1402, 2189
SphI GCATGC 1 cut(s) 1650
SspI AATATT 1 cut(s) 223
SstI GAGCTC 2 cut(s) 23, 2101
StyD4I CCNGG 4 cut(s) 305, 1915, 1965, 2324
StyI CCWWGG 2 cut(s) 1380, 1776
TaaI ACNGT 8 cut(s) 98, 214, 241, 248, 564, 680, 1005, 2284
TaiI ACGT 2 cut(s) 607, 2319
TaqI TCGA 5 cut(s) 132, 137, 287, 829, 2304
TatI WGTACW 1 cut(s) 748
TauI GCSGC 7 cut(s) 29, 32, 359, 377, 401, 1613, 1904
TfiI GAWTC 6 cut(s) 656, 797, 871, 961, 1481, 1594
TscAI CASTG 4 cut(s) 103, 569, 1251, 1428
TspDTI ATGAA 8 cut(s) 525, 585, 648, 854, 864, 981, 2127, 2271
TspGWI ACGGA 2 cut(s) 81, 508
TspRI CASTG 4 cut(s) 103, 569, 1251, 1428
Tth111I GACNNNGTC 1 cut(s) 2294
Vha464I CTTAAG 2 cut(s) 722, 2189
VpaK11BI GGWCC 5 cut(s) 89, 1270, 1799, 2038, 2296
XagI CCTNNNNNAGG 1 cut(s) 1179
XapI RAATTY 4 cut(s) 178, 825, 1514, 1970
XbaI TCTAGA 1 cut(s) 1178
XceI RCATGY 3 cut(s) 1217, 1650, 1744
XmiI GTMKAC 1 cut(s) 1793
XmnI GAANNNNTTC 1 cut(s) 825
Zsp2I ATGCAT 1 cut(s) 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.