RLG00000021494

HAUS augmin-like complex subunit 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010982
Physical Location & Seq
Reverse (-)
75891602 .. 75897888
6287 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021494

Sequence Viewer

Length: 2472 bp
ATGCAGGGCTCGTCGTCGAGCTCTGCGGCTCAGCCGGAGGCCATACTGGAGTGGCTTCAGAAGGAAATGGGGTACCGACCCTTGGGCCCTTACGGCGGCGCCGCCTCCAGCAAATCCCAATTGCCGTCAATCGACGCCGTGAGGAAGATTTGCAGGGGCAACATGATACCCATTTGGAACTTCTTGATCACCCGCGTGAAGTCGGAGAGTACTGTGAAGAATATTCGGAGGAATATTACGGTGCACGGCGACAGCGGCGGCGGCGCGTTGGTGAAGTTGGGGAAGGAGGAGGGGAGGAGTAAAGGGGGGAGAAGGAAGGAGAAGGCTGGGGAGAGTTCGAGTGCGGCGGAGGTCAGGGAGGCGGCGCTGCAGGAGAGGGAGGCGGCGGAGAAGGAGGTGGAGAAGTTGAGGAATATTGTCAAGAGGCAGAGGAAGGATTTGAAGGCTAGAATGCTGGAGGTTTCGAGGGCCGAGGCCGAGAGGAAGAGGATGCTTGATGAAAGATCAAAGAAGAGGCATAAGCAGGTGATGTTGGACGCGTACTATCTACAGTGTGAGGAAGCGGAAAAAATATTTGCAGAGTATCATAAACGTCTCTGTTATTATGTTAATCAAGCCAGGGATTCTCAAAGGTTAAGTGTGGACTCGTCTCTTGAATTGGTTAATAGCTTTAGTTCCAACAGTGAAAAGGAGGCTGTTTATTCAACACTTAGGGGCAGTAAAGCTGCAGATGATGTGCTTCTTATTGAAACCACTCGGGAAAGAAACATACGGAAGGCCTGTGAATCTCTTGCAGCACATATGATTGAAAAGATTCGCAATTCCTTTCCTGCGTATGAAGGAAGTGGTATTCATTTAAGTCCTCAGTTAGAAGCAGCGAAGTTAGGATTTGAATTTGATGGGGAATTACCTGAGGAGGTCAGAGGTGCAATTCTGAATGGCCTGAAGAGTCCTCCTCAGTTGCTTCAGGCAATTACATCATACACATCACGATTGAAATCTCTAATTTCCAGAGAAATAGAGAAAATAGATGTAAGAGCTGATGCAGAAACTTTAAGGTACAAGTACGAAAACAACCGAGTTATAGATGTTTCTTCTCCTGATGTGAGTTCACCTTTACACTATCAACTTTATGGTAATGGAAAGATAGGAGTTGATGCACCATCAAGAGGAACTCAGCTTCTTGAAAGACAGAAAGCCCATGTTCAGCAATTTTTGGCTACTGAAGATGCACTGAACAAAGCTGCAGAAGCTAGAAGTCTGTGTCAAAATCTTATAAAGCGTTTGCATGGAAATAGTGATGGAGTTTCTTCAGGCATATCACAGAATGTAGGCAGTCTTAGGCAACTTGAGTTGGAGGTTTGGGCCAAGGAAAGAGAAGTTGCTGGCTTGAGGGCTAGCTTGAATACGCTAATGTCTGAAATACAGCGCCTGAATAAATTATGTGCAGAAAGAAAAGAAGCTGAAGATTCATTGAAAAAGAAGTGGAAAAAGATTGAAGAATTTGATTCCCGCAGATCTGAACTGGAAACCATATATACTGCTCTACTAAAAGTTAACATGGATGCTGCCGCCTTCTGGAATCAGCAGCCATTAGCCGCAAGGGAGTATGCATCAAGCACTATTATTCCAGCATGCACAATAGTTATGGATCTTTCAAATAATGCAAAAGATCTTATTGAAAAAGAAGTATCTGCTTTTGATCAAAGTCCTGATAATAGTCTCTACATGCTTCCAGCAACTCCACAGGCACTATTGGAGTCAATGGGTGCAAACGGCTCGACTGGACCTGAAGCTGTTGCTGCTGCAGAAAAGAATGCAGCTATATTAACTGCAAAAGCAGGTGCTAGGGATCCATCAGCAATACCATCCATATGTCGTGTATCAGCTGCCCTTCAGTATCCTGGTGGTCTGGAGGGTTCAGATGCTGCTTTAGCATCAGTGTTAGAGTCGCTGGAGTTCTGTTTGAAACTTCGTGGTTCTGAGGCTAGCGTGTTGGAGGACTTAGCAAAGGCAATCAATTTGGTCCATACACGACAGGACCTTGTTGAAAGTGGTCATGTGTTGTTGAACCATGCCTATAGAGCTCAACAAGAATATGATAGGAAAACGAGTTTTTGTTTAAATTTGGCTGCTGAGCAAGAGAGAACTGTCACAGAGAAATGGTTGCCAGAACTTAAGACTGCAATTTTGAATGCTCAGAAGTGCTTGGAAGACTGCAATTATGTTAGAGGATTGCTTGATGAATGGTGGGAGCAACCAGCGGCAACTGTTGTTGACTGGGTCCTAGTTGATGGATTAAATGTCGCTGCTTGGCACAATCATGTGAAGCAGCTTCTTGCATTTTATGATCAGGAGCACATGAGTGCAACGGAGCCATACACTGAGATCACCACAGCCATCATTGGACTTGGGGAAGAGGAGGAGGGTAGCCCCGGCGGAAGGAAGAGGTCTGGGTCCTCTGGGTCTTAG

Protein Analysis

824

Amino Acids

91.16

Weight (kDa)

6.37

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS5 PF14817 12 - 774 1.5e-247 HAUS augmin-like complex subunit 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1277
AarI CACCTGC 2 cut(s) 514, 1832
Acc36I ACCTGC 2 cut(s) 514, 1832
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 2 cut(s) 72, 98
AccII CGCG 3 cut(s) 195, 266, 539
AclWI GGATC 3 cut(s) 1659, 1847, 1860
AcsI RAATTY 3 cut(s) 893, 1502, 2125
AcuI CTGAAG 8 cut(s) 41, 950, 965, 1245, 1296, 1485, 1812, 1880
AcyI GRCGYC 2 cut(s) 99, 135
AfaI GTAC 5 cut(s) 74, 211, 542, 1061, 1067
AfiI CCNNNNNNNGG 5 cut(s) 82, 95, 1169, 1578, 2442
AflII CTTAAG 1 cut(s) 2177
AflIII ACRYGT 1 cut(s) 537
AjnI CCWGG 2 cut(s) 617, 1903
AleI CACNNNNGTG 2 cut(s) 194, 2364
Alw21I GWGCWC 4 cut(s) 23, 246, 2089, 2361
Alw26I GTCTC 3 cut(s) 599, 654, 1727
Alw44I GTGCAC 1 cut(s) 242
AlwI GGATC 3 cut(s) 1659, 1847, 1860
AlwNI CAGNNNCTG 2 cut(s) 1432, 1928
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 7 cut(s) 39, 85, 468, 474, 777, 940, 1365
ApaI GGGCCC 1 cut(s) 89
ApaLI GTGCAC 1 cut(s) 242
ApoI RAATTY 3 cut(s) 893, 1502, 2125
ArsI GACNNNNNNTTYG 2 cut(s) 2173, 2205
Asp700I GAANNNNTTC 1 cut(s) 813
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 4 cut(s) 101, 266, 367, 1431
AspS9I GGNCC 9 cut(s) 85, 86, 468, 1365, 1787, 2026, 2041, 2284, 2457
AsuC2I CCSGG 1 cut(s) 2436
AsuHPI GGTGA 5 cut(s) 181, 283, 538, 1104, 2383
AsuNHI GCTAGC 2 cut(s) 1397, 1988
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 5 cut(s) 1787, 2026, 2041, 2284, 2457
AxyI CCTNAGG 1 cut(s) 912
BaeGI GKGCMC 2 cut(s) 89, 246
BamHI GGATCC 1 cut(s) 1852
BanI GGYRCC 2 cut(s) 72, 98
BanII GRGCYC 4 cut(s) 11, 23, 89, 2089
BbsI GAAGAC 1 cut(s) 2220
Bbv12I GWGCWC 4 cut(s) 23, 246, 2089, 2361
BccI CCATC 7 cut(s) 893, 1171, 1295, 1864, 1876, 2288, 2408
BceAI ACGGC 5 cut(s) 109, 109, 122, 262, 1792
BcgI CGANNNNNNTGC 2 cut(s) 1761, 1795
BciT130I CCWGG 2 cut(s) 619, 1905
BciVI GTATCC 1 cut(s) 1911
BclI TGATCA 3 cut(s) 186, 1702, 2350
BcnI CCSGG 1 cut(s) 2436
BcoDI GTCTC 3 cut(s) 599, 654, 1727
BfaI CTAG 6 cut(s) 447, 1254, 1398, 1848, 1989, 2288
BfmI CTRYAG 6 cut(s) 368, 548, 726, 1245, 1806, 2080
BfoI RGCGCY 3 cut(s) 102, 368, 1432
BfrI CTTAAG 1 cut(s) 2177
BfuAI ACCTGC 2 cut(s) 514, 1832
BfuI GTATCC 1 cut(s) 1911
BglI GCCNNNNNGGC 1 cut(s) 93
BglII AGATCT 2 cut(s) 1517, 1672
BlpI GCTNAGC 2 cut(s) 30, 2136
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 3 cut(s) 619, 1905, 2436
Bme18I GGWCC 5 cut(s) 1787, 2026, 2041, 2284, 2457
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 9 cut(s) 85, 86, 468, 1365, 1787, 2026, 2041, 2284, 2457
BmiI GGNNCC 7 cut(s) 74, 87, 100, 1854, 2285, 2376, 2458
BmrFI CCNGG 3 cut(s) 619, 1905, 2436
BmrI ACTGGG 1 cut(s) 2290
BmsI GCATC 8 cut(s) 480, 1033, 1147, 1219, 1555, 1622, 1915, 1946
BmtI GCTAGC 2 cut(s) 1401, 1992
BmuI ACTGGG 1 cut(s) 2290
BpiI GAAGAC 1 cut(s) 2220
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 5 cut(s) 68, 91, 476, 1934, 1976
Bpu1102I GCTNAGC 2 cut(s) 30, 2136
BpuEI CTTGAG 2 cut(s) 1370, 1411
BpuMI CCSGG 1 cut(s) 2436
BsaBI GATNNNNATC 1 cut(s) 997
BsaHI GRCGYC 2 cut(s) 99, 135
BsaJI CCNNGG 5 cut(s) 81, 471, 618, 1368, 2434
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 95, 1169, 1578, 2442
Bse1I ACTGG 4 cut(s) 51, 1530, 1789, 2285
Bse21I CCTNAGG 1 cut(s) 912
Bse8I GATNNNNATC 1 cut(s) 997
BseBI CCWGG 2 cut(s) 619, 1905
BseDI CCNNGG 5 cut(s) 81, 471, 618, 1368, 2434
BseGI GGATG 3 cut(s) 495, 1570, 1868
BseJI GATNNNNATC 1 cut(s) 997
BseLI CCNNNNNNNGG 5 cut(s) 82, 95, 1169, 1578, 2442
BseMII CTCAG 9 cut(s) 44, 878, 903, 971, 1190, 1974, 2127, 2213, 2376
BseNI ACTGG 4 cut(s) 51, 1530, 1789, 2285
BseRI GAGGAG 6 cut(s) 302, 310, 929, 945, 2435, 2438
BseSI GKGCMC 2 cut(s) 89, 246
BseYI CCCAGC 1 cut(s) 326
BsgI GTGCAG 1 cut(s) 1467
Bsh1236I CGCG 3 cut(s) 195, 266, 539
BshFI GGCC 7 cut(s) 41, 87, 470, 476, 779, 942, 1367
BshNI GGYRCC 2 cut(s) 72, 98
BsiHKAI GWGCWC 4 cut(s) 23, 246, 2089, 2361
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 2 cut(s) 35, 2436
BslI CCNNNNNNNGG 5 cut(s) 82, 95, 1169, 1578, 2442
BsmAI GTCTC 3 cut(s) 599, 654, 1727
BsmBI CGTCTC 2 cut(s) 599, 654
BsmI GAATGC 3 cut(s) 456, 1822, 2200
BsnI GGCC 7 cut(s) 41, 87, 470, 476, 779, 942, 1367
BsoBI CYCGRG 1 cut(s) 756
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 6 cut(s) 11, 23, 89, 246, 2089, 2361
Bsp143I GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
Bsp1720I GCTNAGC 2 cut(s) 30, 2136
BspANI GGCC 7 cut(s) 41, 87, 470, 476, 779, 942, 1367
BspCNI CTCAG 9 cut(s) 43, 877, 904, 970, 1189, 1975, 2128, 2212, 2377
BspFNI CGCG 3 cut(s) 195, 266, 539
BspLI GGNNCC 7 cut(s) 74, 87, 100, 1854, 2285, 2376, 2458
BspMAI CTGCAG 4 cut(s) 372, 730, 1249, 1810
BspMI ACCTGC 2 cut(s) 514, 1832
BspOI GCTAGC 2 cut(s) 1401, 1992
BspPI GGATC 3 cut(s) 1659, 1847, 1860
BspT107I GGYRCC 2 cut(s) 72, 98
BspTI CTTAAG 1 cut(s) 2177
BsrI ACTGG 4 cut(s) 51, 1530, 1789, 2285
BssECI CCNNGG 5 cut(s) 81, 471, 618, 1368, 2434
BssMI GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
BssNI GRCGYC 2 cut(s) 99, 135
BssT1I CCWWGG 2 cut(s) 81, 1368
Bst2UI CCWGG 2 cut(s) 619, 1905
Bst4CI ACNGT 6 cut(s) 214, 241, 552, 683, 2152, 2272
Bst6I CTCTTC 5 cut(s) 479, 506, 941, 2412, 2441
BstACI GRCGYC 2 cut(s) 99, 135
BstAFI CTTAAG 1 cut(s) 2177
BstC8I GCNNGC 4 cut(s) 1387, 1399, 1636, 1990
BstF5I GGATG 3 cut(s) 495, 1570, 1868
BstFNI CGCG 3 cut(s) 195, 266, 539
BstH2I RGCGCY 3 cut(s) 102, 368, 1432
BstHHI GCGC 4 cut(s) 101, 266, 367, 1431
BstKTI GATC 9 cut(s) 189, 506, 1520, 1654, 1675, 1705, 1855, 2353, 2391
BstMAI GTCTC 3 cut(s) 599, 654, 1727
BstMBI GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
BstMWI GCNNNNNNNGC 7 cut(s) 93, 255, 261, 1250, 1802, 1934, 2084
BstNI CCWGG 2 cut(s) 619, 1905
BstNSI RCATGY 2 cut(s) 1638, 1732
BstSCI CCNGG 3 cut(s) 617, 1903, 2434
BstSFI CTRYAG 6 cut(s) 368, 548, 726, 1245, 1806, 2080
BstSLI GKGCMC 2 cut(s) 89, 246
BstUI CGCG 3 cut(s) 195, 266, 539
BstV2I GAAGAC 1 cut(s) 2220
BstX2I RGATCY 4 cut(s) 1517, 1651, 1672, 1852
BstYI RGATCY 4 cut(s) 1517, 1651, 1672, 1852
Bsu36I CCTNAGG 1 cut(s) 912
BsuI GTATCC 1 cut(s) 1911
BsuRI GGCC 7 cut(s) 41, 87, 470, 476, 779, 942, 1367
BtsCI GGATG 3 cut(s) 495, 1570, 1868
BtsIMutI CAGTG 5 cut(s) 557, 688, 1232, 1947, 2382
BveI ACCTGC 2 cut(s) 514, 1832
Cac8I GCNNGC 4 cut(s) 1387, 1399, 1636, 1990
CaiI CAGNNNCTG 2 cut(s) 1432, 1928
CfoI GCGC 4 cut(s) 101, 266, 367, 1431
Cfr13I GGNCC 9 cut(s) 85, 86, 468, 1365, 1787, 2026, 2041, 2284, 2457
CseI GACGC 2 cut(s) 143, 545
Csp6I GTAC 5 cut(s) 73, 210, 541, 1060, 1066
CviQI GTAC 5 cut(s) 73, 210, 541, 1060, 1066
DinI GGCGCC 1 cut(s) 100
DpnI GATC 9 cut(s) 188, 505, 1519, 1653, 1674, 1704, 1854, 2352, 2390
DpnII GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
DraI TTTAAA 1 cut(s) 2124
Eam1104I CTCTTC 5 cut(s) 479, 506, 941, 2412, 2441
EarI CTCTTC 5 cut(s) 479, 506, 941, 2412, 2441
EciI GGCGGA 3 cut(s) 362, 401, 2454
Ecl136II GAGCTC 2 cut(s) 21, 2087
Eco130I CCWWGG 2 cut(s) 81, 1368
Eco147I AGGCCT 1 cut(s) 779
Eco24I GRGCYC 4 cut(s) 11, 23, 89, 2089
Eco47I GGWCC 5 cut(s) 1787, 2026, 2041, 2284, 2457
Eco53kI GAGCTC 2 cut(s) 21, 2087
Eco57I CTGAAG 8 cut(s) 41, 950, 965, 1245, 1296, 1485, 1812, 1880
Eco81I CCTNAGG 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 756
EcoICRI GAGCTC 2 cut(s) 21, 2087
EcoO109I RGGNCCY 4 cut(s) 86, 2041, 2284, 2457
EcoRII CCWGG 2 cut(s) 617, 1903
EcoT14I CCWWGG 2 cut(s) 81, 1368
EcoT22I ATGCAT 1 cut(s) 1615
EcoT38I GRGCYC 4 cut(s) 11, 23, 89, 2089
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 81, 1368
Esp3I CGTCTC 2 cut(s) 599, 654
FauI CCCGC 2 cut(s) 200, 1520
FauNDI CATATG 2 cut(s) 801, 1874
FbaI TGATCA 3 cut(s) 186, 1702, 2350
FokI GGATG 3 cut(s) 502, 1577, 1855
FriOI GRGCYC 4 cut(s) 11, 23, 89, 2089
FspBI CTAG 6 cut(s) 447, 1254, 1398, 1848, 1989, 2288
GlaI GCGC 4 cut(s) 100, 265, 366, 1430
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 5 cut(s) 68, 91, 476, 1934, 1976
HaeII RGCGCY 3 cut(s) 102, 368, 1432
HaeIII GGCC 7 cut(s) 41, 87, 470, 476, 779, 942, 1367
HapII CCGG 2 cut(s) 35, 2436
HgaI GACGC 2 cut(s) 143, 545
HhaI GCGC 4 cut(s) 101, 266, 367, 1431
Hin1I GRCGYC 2 cut(s) 99, 135
Hin6I GCGC 4 cut(s) 99, 264, 365, 1429
HinP1I GCGC 4 cut(s) 99, 264, 365, 1429
HincII GTYRAC 2 cut(s) 1558, 2278
HindII GTYRAC 2 cut(s) 1558, 2278
HpaI GTTAAC 1 cut(s) 1558
HpaII CCGG 2 cut(s) 35, 2436
HphI GGTGA 5 cut(s) 181, 283, 538, 1104, 2383
Hpy166II GTNNAC 5 cut(s) 244, 643, 1112, 1558, 2278
Hpy8I GTNNAC 5 cut(s) 244, 643, 1112, 1558, 2278
Hpy99I CGWCG 3 cut(s) 16, 19, 137
HpyCH4III ACNGT 6 cut(s) 214, 241, 552, 683, 2152, 2272
HpyCH4IV ACGT 1 cut(s) 592
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 255, 261, 1250, 1802, 1934, 2084
HpySE526I ACGT 1 cut(s) 592
Hsp92I GRCGYC 2 cut(s) 99, 135
HspAI GCGC 4 cut(s) 99, 264, 365, 1429
KasI GGCGCC 1 cut(s) 98
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 3 cut(s) 186, 1702, 2350
KspAI GTTAAC 1 cut(s) 1558
Kzo9I GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
LmnI GCTCC 3 cut(s) 2254, 2356, 2374
LweI GCATC 8 cut(s) 480, 1033, 1147, 1219, 1555, 1622, 1915, 1946
MaeI CTAG 6 cut(s) 447, 1254, 1398, 1848, 1989, 2288
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 1 cut(s) 2152
MalI GATC 9 cut(s) 188, 505, 1519, 1653, 1674, 1704, 1854, 2352, 2390
MboI GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 4 cut(s) 1517, 1651, 1672, 1852
MhlI GDGCHC 6 cut(s) 11, 23, 89, 246, 2089, 2361
MluI ACGCGT 1 cut(s) 537
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 4 cut(s) 638, 958, 1769, 1958
MmeI TCCRAC 5 cut(s) 183, 513, 702, 1335, 1977
Mph1103I ATGCAT 1 cut(s) 1615
MroXI GAANNNNTTC 1 cut(s) 813
MslI CAYNNNNRTG 4 cut(s) 194, 1873, 2322, 2364
MspA1I CMGCKG 3 cut(s) 255, 1889, 2264
MspCI CTTAAG 1 cut(s) 2177
MspI CCGG 2 cut(s) 35, 2436
MspR9I CCNGG 3 cut(s) 619, 1905, 2436
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 3 cut(s) 456, 1822, 2200
MvaI CCWGG 2 cut(s) 619, 1905
MvnI CGCG 3 cut(s) 195, 266, 539
MwoI GCNNNNNNNGC 7 cut(s) 93, 255, 261, 1250, 1802, 1934, 2084
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 1 cut(s) 2436
NdeI CATATG 2 cut(s) 801, 1874
NdeII GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
NheI GCTAGC 2 cut(s) 1397, 1988
NlaIV GGNNCC 7 cut(s) 74, 87, 100, 1854, 2285, 2376, 2458
NmeAIII GCCGAG 2 cut(s) 496, 502
NmuCI GTSAC 1 cut(s) 2152
NsiI ATGCAT 1 cut(s) 1615
NspI RCATGY 2 cut(s) 1638, 1732
OliI CACNNNNGTG 2 cut(s) 194, 2364
PaeI GCATGC 1 cut(s) 1638
PaqCI CACCTGC 2 cut(s) 514, 1832
PceI AGGCCT 1 cut(s) 779
PctI GAATGC 3 cut(s) 456, 1822, 2200
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 623, 785, 814, 1469, 1508, 1582
PflFI GACNNNGTC 1 cut(s) 2282
PleI GAGTC 4 cut(s) 638, 957, 1768, 1957
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 4 cut(s) 638, 957, 1768, 1957
PpuMI RGGWCCY 3 cut(s) 2041, 2284, 2457
PsiI TTATAA 1 cut(s) 1277
Psp124BI GAGCTC 2 cut(s) 23, 2089
Psp5II RGGWCCY 3 cut(s) 2041, 2284, 2457
Psp6I CCWGG 2 cut(s) 617, 1903
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 617, 1903
PspN4I GGNNCC 7 cut(s) 74, 87, 100, 1854, 2285, 2376, 2458
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 9 cut(s) 85, 86, 468, 1365, 1787, 2026, 2041, 2284, 2457
PspPPI RGGWCCY 3 cut(s) 2041, 2284, 2457
PstI CTGCAG 4 cut(s) 372, 730, 1249, 1810
PstNI CAGNNNCTG 2 cut(s) 1432, 1928
PsuI RGATCY 4 cut(s) 1517, 1651, 1672, 1852
PsyI GACNNNGTC 1 cut(s) 2282
PvuII CAGCTG 1 cut(s) 1889
RsaI GTAC 5 cut(s) 74, 211, 542, 1061, 1067
RsaNI GTAC 5 cut(s) 73, 210, 541, 1060, 1066
RseI CAYNNNNRTG 4 cut(s) 194, 1873, 2322, 2364
SacI GAGCTC 2 cut(s) 23, 2089
Sau3AI GATC 9 cut(s) 186, 503, 1517, 1651, 1672, 1702, 1852, 2350, 2388
Sau96I GGNCC 9 cut(s) 85, 86, 468, 1365, 1787, 2026, 2041, 2284, 2457
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 4 cut(s) 638, 958, 1769, 1958
ScrFI CCNGG 3 cut(s) 619, 1905, 2436
SduI GDGCHC 6 cut(s) 11, 23, 89, 246, 2089, 2361
SfaNI GCATC 8 cut(s) 480, 1033, 1147, 1219, 1555, 1622, 1915, 1946
SfcI CTRYAG 6 cut(s) 368, 548, 726, 1245, 1806, 2080
SfoI GGCGCC 1 cut(s) 100
SinI GGWCC 5 cut(s) 1787, 2026, 2041, 2284, 2457
SmiMI CAYNNNNRTG 4 cut(s) 194, 1873, 2322, 2364
SmlI CTYRAG 3 cut(s) 1349, 1390, 2177
SmoI CTYRAG 3 cut(s) 1349, 1390, 2177
SphI GCATGC 1 cut(s) 1638
SseBI AGGCCT 1 cut(s) 779
SspDI GGCGCC 1 cut(s) 98
SspI AATATT 4 cut(s) 223, 235, 415, 573
SspMI CTAG 6 cut(s) 447, 1254, 1398, 1848, 1989, 2288
SstI GAGCTC 2 cut(s) 23, 2089
StuI AGGCCT 1 cut(s) 779
StyD4I CCNGG 3 cut(s) 617, 1903, 2434
StyI CCWWGG 2 cut(s) 81, 1368
TaaI ACNGT 6 cut(s) 214, 241, 552, 683, 2152, 2272
TaiI ACGT 1 cut(s) 595
TaqI TCGA 5 cut(s) 17, 132, 338, 464, 1781
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 6 cut(s) 623, 785, 814, 1469, 1508, 1582
TscAI CASTG 5 cut(s) 557, 688, 1239, 1947, 2389
TseFI GTSAC 1 cut(s) 2152
Tsp45I GTSAC 1 cut(s) 2152
TspDTI ATGAA 5 cut(s) 513, 842, 852, 1461, 2259
TspGWI ACGGA 2 cut(s) 787, 2387
TspRI CASTG 5 cut(s) 557, 688, 1239, 1947, 2389
Tth111I GACNNNGTC 1 cut(s) 2282
Vha464I CTTAAG 1 cut(s) 2177
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 5 cut(s) 1787, 2026, 2041, 2284, 2457
XapI RAATTY 3 cut(s) 893, 1502, 2125
XceI RCATGY 2 cut(s) 1638, 1732
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 6 cut(s) 447, 1254, 1398, 1848, 1989, 2288
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.