Rh2DG600500

HAUS augmin-like complex subunit 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010982
Physical Location & Seq
Reverse (-)
83270528 .. 83277316
6789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG600500.1

Sequence Viewer

Length: 2331 bp
ATGCAGGGCTCGTCGTCGAGCTCTGCGGCTCAGCCGGAGGCCATACTGGAGTGGCTTCAGAAGGAAATGGGGTACCGACCCTTGGGCCCTTACGGCGGCGCCGCCTCCAGCAAATCCCAATTGCCGTCAATCGACGCCGTGAGGAAGATTTGCAGGGGGAACATGATACCCATTTGGAACTTCTTGATCACGCGCGTGAAGTCGGAGAGTACTGTGAAGAATATTCGGAGGAATATTACGGTGCACGGCGACAGCGGCGGCGCGTTGGTGAAGTTGGGGAAGGAGGAGGGGAGGAGTAAAGGGGGGAGGAGGAAGGAGAAGGCTGGGGAGAGTTCGAGTGCGGCGGAGGTCAGGGAGGCGGCGCTGCAGGAGAGGGTGGCGGCGGAGAAGGAGGTGGAGAAGTTGAGGAATATTGTCAAGAGGCAGAGGAAGGATTTGAAGGCTAGAATGCTGGAGGTTTCGAGGGCCGAGGCCGAGAGGAAGAGGATGCTTGATGAAAGATCAAAGAAAAGGCATAAGCAGGTGATGTTGGACGCGTACTATCTACAGTGTGAGGAAGCGGAAAAAATATTTGCAGAGTATCATAAACGTCTCTGTTATTATGTTAATCAAGCCAGGGATTCTCAAAGGTTAAGTGTGGACTCGTCTCTTGAATTGGTTAATAGCTTTAGTTCCAACAGTGAAAAGGAGGCTGTTTATTCAACACTTAGGGGCAGTAAAGCTGCAGATGATGTGCTTCTTATTGAAACCACTCGGGAAAGAAACATACGGAAGGCCTGTGAATCTCTTGCAGCGCATATGATTGAAAAGATTCGCAATTCCTTTCCTGCGTATGAAGGAAGTGGTATTCATTTAAGTCCTCAGTTAGAAGCAGCCAAGTTAGGATTTGAATTTGATGGGGAATTACCTGAGGAGGTCAGAGGTGCAATTCTGAATGGCCTGAAGAGTCCTCCTCAGTTGCTTCAGGCAATTACGTCATACACATCACGATTGAAATCTCTAATTTCCAGAGAAATAGAGAAAATAGATGTAAGAGCTGATGCAGAAACTTTAAGGTACAAGTATGAAAACAACCGAGTTATAGATGTTTCTTCTCCTGATGTGAGTTCACCTTTACACTATCAACTTTATGGTAATGGAAAGATAGGAGTTGATGCACCATCAAGAGGAACTCAGCTTCTTGAAAGACAGAATAGTCGGGCTAAACCCCAATGCCATAGAAGATCATACTGTTTAAATAAAGCCCATGTTCAGCAATTTTTGGCTACTGAAGATGCACTGAACAAAGCTGCAGAAGCTAGGAGTCTGTGTCAAAATCTTTTAAAGCGTTTGCATGGAAATGGTGATGGAGTTTCTTCAGGCATATCACAGAATGTAGGCAGTCTTAGGCAACTTGAGTTGGAGGTTTGGGCCAAGGAAAGAGAAGTTGCCGGCTTGAGGGCTAGCTTGAATACGCTAATGTCTGAAATACAGCGCCTGAATAAATTATGTGCAGAAAGAAAAGAAGCTGAAGATTCATTGAAAAAGAAGTGGAAAAAGATTGAAGAATTTGATTCCCGCAGATCTGAACTGGAAACCATATATACTGCTCTACTAAAAGTTAACATGGATGCTGCCGCCTTCTGGAATCAGCAGCCATTAGCTGCAAGGGAGTATGCATCAAGCACTATTATTCCAGCATGCACAATAGTTATGGATCTTTCAAATAATGCAAAAGATCTTATTGAAAAAGAAGTATCTGCTTTTGATCAAAGTCCTGATAATAGTCTCTACATGCTTCCAGCAACTCCACAGGCACTATTGGAGTCAATGGGTGCAAACGGCTCGACTGGACCTGAAGCTGTTGCTGCTGCAGAAAAGAATGCAGCTATATTAACTGCAAAAGCAGGTGCTAGGGATCCATCAGCAATACCATCCATATGTCGTGTATCAGCCGCCCTTCAGTATCCTGGTGGTCTGGAGGGTTCAGATGCTGCTTTAGCATCAGTGTTAGAGTCGCTGGAGTTCTGTTTGAAACTTCGTGGTTCTGAGGCTAGCGTGTTGGAGGACTTAGCAAAGGCAATCAATTTGGTCCATACACGACAGGACCTTGTTGAAAGTGGTCATGTGTTGTTGAACCATGCCTATAGAGCTCAACAAGAATATGATAGGAAAACGAGTTTTTGTTTAAATTTGGCTGCTGAGCAAGAGAGAACTGTCACAGAGAAATGGTTGCCAGAACTTAAGACTGCAATTTTGAATGCTCAGAAGTGCTTGGAAGACTGCAATTATGTTAGAGGATTGGTTAGGAGGTTGGGGTGGATTCATATGTCTAGATATTACTTTCCATAA

Protein Analysis

776

Amino Acids

86.47

Weight (kDa)

8.85

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS5 PF14817 12 - 763 9e-223 HAUS augmin-like complex subunit 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 511, 1877
AasI GACNNNNNNGTC 1 cut(s) 1194
Acc36I ACCTGC 2 cut(s) 511, 1877
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 2 cut(s) 72, 98
AccII CGCG 4 cut(s) 193, 195, 263, 536
AclWI GGATC 3 cut(s) 1704, 1892, 1905
AcsI RAATTY 3 cut(s) 890, 1547, 2170
AcuI CTGAAG 8 cut(s) 41, 947, 962, 1290, 1341, 1530, 1857, 1925
AcyI GRCGYC 2 cut(s) 99, 135
AfaI GTAC 4 cut(s) 74, 211, 539, 1058
AfiI CCNNNNNNNGG 5 cut(s) 82, 95, 1166, 1437, 1623
AflII CTTAAG 1 cut(s) 2222
AflIII ACRYGT 1 cut(s) 534
AjnI CCWGG 2 cut(s) 614, 1948
AleI CACNNNNGTG 1 cut(s) 194
Alw21I GWGCWC 3 cut(s) 23, 246, 2134
Alw26I GTCTC 3 cut(s) 596, 651, 1772
Alw44I GTGCAC 1 cut(s) 242
AlwI GGATC 3 cut(s) 1704, 1892, 1905
AlwNI CAGNNNCTG 2 cut(s) 1477, 1973
Ama87I CYCGRG 1 cut(s) 753
AoxI GGCC 7 cut(s) 39, 85, 465, 471, 774, 937, 1410
ApaI GGGCCC 1 cut(s) 89
ApaLI GTGCAC 1 cut(s) 242
ApoI RAATTY 3 cut(s) 890, 1547, 2170
ArsI GACNNNNNNTTYG 2 cut(s) 2218, 2250
Asp700I GAANNNNTTC 1 cut(s) 810
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 6 cut(s) 101, 195, 263, 364, 796, 1476
AspS9I GGNCC 7 cut(s) 85, 86, 465, 1410, 1832, 2071, 2086
AsuHPI GGTGA 4 cut(s) 280, 535, 1101, 1355
AsuNHI GCTAGC 2 cut(s) 1442, 2033
AvaI CYCGRG 1 cut(s) 753
AvaII GGWCC 3 cut(s) 1832, 2071, 2086
AxyI CCTNAGG 1 cut(s) 909
BaeGI GKGCMC 2 cut(s) 89, 246
BamHI GGATCC 1 cut(s) 1897
BanI GGYRCC 2 cut(s) 72, 98
BanII GRGCYC 4 cut(s) 11, 23, 89, 2134
BbsI GAAGAC 1 cut(s) 2265
Bbv12I GWGCWC 3 cut(s) 23, 246, 2134
BccI CCATC 5 cut(s) 890, 1168, 1340, 1909, 1921
BceAI ACGGC 5 cut(s) 109, 109, 122, 262, 1837
BcgI CGANNNNNNTGC 2 cut(s) 1806, 1840
BciT130I CCWGG 2 cut(s) 616, 1950
BciVI GTATCC 1 cut(s) 1956
BclI TGATCA 2 cut(s) 186, 1747
BcoDI GTCTC 3 cut(s) 596, 651, 1772
BfaI CTAG 6 cut(s) 444, 1299, 1443, 1893, 2034, 2313
BfmI CTRYAG 6 cut(s) 365, 545, 723, 1290, 1851, 2125
BfoI RGCGCY 3 cut(s) 102, 365, 1477
BfrI CTTAAG 1 cut(s) 2222
BfuAI ACCTGC 2 cut(s) 511, 1877
BfuI GTATCC 1 cut(s) 1956
BglI GCCNNNNNGGC 1 cut(s) 93
BglII AGATCT 2 cut(s) 1562, 1717
BlpI GCTNAGC 2 cut(s) 30, 2181
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 616, 1950
Bme18I GGWCC 3 cut(s) 1832, 2071, 2086
BmeT110I CYCGRG 1 cut(s) 753
BmgT120I GGNCC 7 cut(s) 85, 86, 465, 1410, 1832, 2071, 2086
BmiI GGNNCC 4 cut(s) 74, 87, 100, 1899
BmrFI CCNGG 2 cut(s) 616, 1950
BmsI GCATC 8 cut(s) 477, 1030, 1144, 1264, 1600, 1667, 1960, 1991
BmtI GCTAGC 2 cut(s) 1446, 2037
BpiI GAAGAC 1 cut(s) 2265
BplI GAGNNNNNCTC 2 cut(s) 937, 969
BpmI CTGGAG 5 cut(s) 68, 91, 473, 1979, 2021
Bpu1102I GCTNAGC 2 cut(s) 30, 2181
BpuEI CTTGAG 2 cut(s) 1415, 1456
BsaBI GATNNNNATC 1 cut(s) 994
BsaHI GRCGYC 2 cut(s) 99, 135
BsaJI CCNNGG 4 cut(s) 81, 468, 615, 1413
BsaXI ACNNNNNCTCC 2 cut(s) 1294, 1324
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 95, 1166, 1437, 1623
Bse118I RCCGGY 1 cut(s) 1430
Bse1I ACTGG 3 cut(s) 51, 1575, 1834
Bse21I CCTNAGG 1 cut(s) 909
Bse8I GATNNNNATC 1 cut(s) 994
BseBI CCWGG 2 cut(s) 616, 1950
BseDI CCNNGG 4 cut(s) 81, 468, 615, 1413
BseGI GGATG 3 cut(s) 492, 1615, 1913
BseJI GATNNNNATC 1 cut(s) 994
BseLI CCNNNNNNNGG 5 cut(s) 82, 95, 1166, 1437, 1623
BseMII CTCAG 8 cut(s) 44, 875, 900, 968, 1187, 2019, 2172, 2258
BseNI ACTGG 3 cut(s) 51, 1575, 1834
BseRI GAGGAG 5 cut(s) 299, 307, 322, 926, 942
BseSI GKGCMC 2 cut(s) 89, 246
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 1512
Bsh1236I CGCG 4 cut(s) 193, 195, 263, 536
BshFI GGCC 7 cut(s) 41, 87, 467, 473, 776, 939, 1412
BshNI GGYRCC 2 cut(s) 72, 98
BsiHKAI GWGCWC 3 cut(s) 23, 246, 2134
BsiHKCI CYCGRG 1 cut(s) 753
BsiSI CCGG 2 cut(s) 35, 1431
BslI CCNNNNNNNGG 5 cut(s) 82, 95, 1166, 1437, 1623
BsmAI GTCTC 3 cut(s) 596, 651, 1772
BsmBI CGTCTC 2 cut(s) 596, 651
BsmI GAATGC 3 cut(s) 453, 1867, 2245
BsnI GGCC 7 cut(s) 41, 87, 467, 473, 776, 939, 1412
BsoBI CYCGRG 1 cut(s) 753
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 5 cut(s) 11, 23, 89, 246, 2134
Bsp143I GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
Bsp1720I GCTNAGC 2 cut(s) 30, 2181
BspANI GGCC 7 cut(s) 41, 87, 467, 473, 776, 939, 1412
BspCNI CTCAG 8 cut(s) 43, 874, 901, 967, 1186, 2020, 2173, 2257
BspFNI CGCG 4 cut(s) 193, 195, 263, 536
BspLI GGNNCC 4 cut(s) 74, 87, 100, 1899
BspMAI CTGCAG 4 cut(s) 369, 727, 1294, 1855
BspMI ACCTGC 2 cut(s) 511, 1877
BspOI GCTAGC 2 cut(s) 1446, 2037
BspPI GGATC 3 cut(s) 1704, 1892, 1905
BspT107I GGYRCC 2 cut(s) 72, 98
BspTI CTTAAG 1 cut(s) 2222
BsrFI RCCGGY 1 cut(s) 1430
BsrI ACTGG 3 cut(s) 51, 1575, 1834
BssAI RCCGGY 1 cut(s) 1430
BssECI CCNNGG 4 cut(s) 81, 468, 615, 1413
BssMI GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
BssNI GRCGYC 2 cut(s) 99, 135
BssT1I CCWWGG 2 cut(s) 81, 1413
Bst2UI CCWGG 2 cut(s) 616, 1950
Bst4CI ACNGT 6 cut(s) 214, 241, 549, 680, 1232, 2197
Bst6I CTCTTC 2 cut(s) 476, 938
BstACI GRCGYC 2 cut(s) 99, 135
BstAFI CTTAAG 1 cut(s) 2222
BstC8I GCNNGC 4 cut(s) 1432, 1444, 1681, 2035
BstF5I GGATG 3 cut(s) 492, 1615, 1913
BstFNI CGCG 4 cut(s) 193, 195, 263, 536
BstH2I RGCGCY 3 cut(s) 102, 365, 1477
BstHHI GCGC 6 cut(s) 101, 195, 263, 364, 796, 1476
BstKTI GATC 8 cut(s) 189, 503, 1226, 1565, 1699, 1720, 1750, 1900
BstMAI GTCTC 3 cut(s) 596, 651, 1772
BstMBI GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
BstMWI GCNNNNNNNGC 6 cut(s) 93, 255, 1295, 1847, 1979, 2129
BstNI CCWGG 2 cut(s) 616, 1950
BstNSI RCATGY 2 cut(s) 1683, 1777
BstSCI CCNGG 2 cut(s) 614, 1948
BstSFI CTRYAG 6 cut(s) 365, 545, 723, 1290, 1851, 2125
BstSLI GKGCMC 2 cut(s) 89, 246
BstUI CGCG 4 cut(s) 193, 195, 263, 536
BstV2I GAAGAC 1 cut(s) 2265
BstX2I RGATCY 4 cut(s) 1562, 1696, 1717, 1897
BstYI RGATCY 4 cut(s) 1562, 1696, 1717, 1897
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 1956
BsuRI GGCC 7 cut(s) 41, 87, 467, 473, 776, 939, 1412
BtsCI GGATG 3 cut(s) 492, 1615, 1913
BtsIMutI CAGTG 4 cut(s) 554, 685, 1277, 1992
BveI ACCTGC 2 cut(s) 511, 1877
Cac8I GCNNGC 4 cut(s) 1432, 1444, 1681, 2035
CaiI CAGNNNCTG 2 cut(s) 1477, 1973
CfoI GCGC 6 cut(s) 101, 195, 263, 364, 796, 1476
Cfr10I RCCGGY 1 cut(s) 1430
Cfr13I GGNCC 7 cut(s) 85, 86, 465, 1410, 1832, 2071, 2086
CseI GACGC 2 cut(s) 143, 542
Csp6I GTAC 4 cut(s) 73, 210, 538, 1057
CviAII CATG 8 cut(s) 163, 1247, 1334, 1606, 1680, 1774, 2105, 2120
CviQI GTAC 4 cut(s) 73, 210, 538, 1057
DinI GGCGCC 1 cut(s) 100
DpnI GATC 8 cut(s) 188, 502, 1225, 1564, 1698, 1719, 1749, 1899
DpnII GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
DraI TTTAAA 3 cut(s) 1236, 1323, 2169
DrdI GACNNNNNNGTC 1 cut(s) 1194
DseDI GACNNNNNNGTC 1 cut(s) 1194
Eam1104I CTCTTC 2 cut(s) 476, 938
EarI CTCTTC 2 cut(s) 476, 938
EciI GGCGGA 2 cut(s) 359, 398
Ecl136II GAGCTC 2 cut(s) 21, 2132
Eco130I CCWWGG 2 cut(s) 81, 1413
Eco147I AGGCCT 1 cut(s) 776
Eco24I GRGCYC 4 cut(s) 11, 23, 89, 2134
Eco47I GGWCC 3 cut(s) 1832, 2071, 2086
Eco53kI GAGCTC 2 cut(s) 21, 2132
Eco57I CTGAAG 8 cut(s) 41, 947, 962, 1290, 1341, 1530, 1857, 1925
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 753
EcoICRI GAGCTC 2 cut(s) 21, 2132
EcoO109I RGGNCCY 2 cut(s) 86, 2086
EcoRII CCWGG 2 cut(s) 614, 1948
EcoT14I CCWWGG 2 cut(s) 81, 1413
EcoT22I ATGCAT 1 cut(s) 1660
EcoT38I GRGCYC 4 cut(s) 11, 23, 89, 2134
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 81, 1413
Esp3I CGTCTC 2 cut(s) 596, 651
FaeI CATG 8 cut(s) 166, 1250, 1337, 1609, 1683, 1777, 2108, 2123
FatI CATG 8 cut(s) 162, 1246, 1333, 1605, 1679, 1773, 2104, 2119
FauI CCCGC 1 cut(s) 1565
FauNDI CATATG 3 cut(s) 798, 1919, 2307
FbaI TGATCA 2 cut(s) 186, 1747
FokI GGATG 3 cut(s) 499, 1622, 1900
FriOI GRGCYC 4 cut(s) 11, 23, 89, 2134
FspBI CTAG 6 cut(s) 444, 1299, 1443, 1893, 2034, 2313
GlaI GCGC 6 cut(s) 100, 194, 262, 363, 795, 1475
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 5 cut(s) 68, 91, 473, 1979, 2021
HaeII RGCGCY 3 cut(s) 102, 365, 1477
HaeIII GGCC 7 cut(s) 41, 87, 467, 473, 776, 939, 1412
HapII CCGG 2 cut(s) 35, 1431
HgaI GACGC 2 cut(s) 143, 542
HhaI GCGC 6 cut(s) 101, 195, 263, 364, 796, 1476
Hin1I GRCGYC 2 cut(s) 99, 135
Hin1II CATG 8 cut(s) 166, 1250, 1337, 1609, 1683, 1777, 2108, 2123
Hin6I GCGC 6 cut(s) 99, 193, 261, 362, 794, 1474
HinP1I GCGC 6 cut(s) 99, 193, 261, 362, 794, 1474
HincII GTYRAC 1 cut(s) 1603
HindII GTYRAC 1 cut(s) 1603
HpaI GTTAAC 1 cut(s) 1603
HpaII CCGG 2 cut(s) 35, 1431
HphI GGTGA 4 cut(s) 280, 535, 1101, 1355
Hpy166II GTNNAC 4 cut(s) 244, 640, 1109, 1603
Hpy8I GTNNAC 4 cut(s) 244, 640, 1109, 1603
Hpy99I CGWCG 3 cut(s) 16, 19, 137
HpyCH4III ACNGT 6 cut(s) 214, 241, 549, 680, 1232, 2197
HpyCH4IV ACGT 2 cut(s) 589, 974
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 255, 1295, 1847, 1979, 2129
HpySE526I ACGT 2 cut(s) 589, 974
Hsp92I GRCGYC 2 cut(s) 99, 135
Hsp92II CATG 8 cut(s) 166, 1250, 1337, 1609, 1683, 1777, 2108, 2123
HspAI GCGC 6 cut(s) 99, 193, 261, 362, 794, 1474
KasI GGCGCC 1 cut(s) 98
KpnI GGTACC 1 cut(s) 76
KroI GCCGGC 1 cut(s) 1430
KroNI GCCGGC 1 cut(s) 1432
Ksp22I TGATCA 2 cut(s) 186, 1747
KspAI GTTAAC 1 cut(s) 1603
Kzo9I GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
LweI GCATC 8 cut(s) 477, 1030, 1144, 1264, 1600, 1667, 1960, 1991
MaeI CTAG 6 cut(s) 444, 1299, 1443, 1893, 2034, 2313
MaeII ACGT 2 cut(s) 589, 974
MaeIII GTNAC 1 cut(s) 2197
MalI GATC 8 cut(s) 188, 502, 1225, 1564, 1698, 1719, 1749, 1899
MboI GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 4 cut(s) 1562, 1696, 1717, 1897
MhlI GDGCHC 5 cut(s) 11, 23, 89, 246, 2134
MluI ACGCGT 1 cut(s) 534
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 5 cut(s) 635, 955, 1312, 1814, 2003
MmeI TCCRAC 5 cut(s) 183, 510, 699, 1380, 2022
Mph1103I ATGCAT 1 cut(s) 1660
MroNI GCCGGC 1 cut(s) 1430
MroXI GAANNNNTTC 1 cut(s) 810
MslI CAYNNNNRTG 3 cut(s) 194, 1338, 1918
MspA1I CMGCKG 1 cut(s) 255
MspCI CTTAAG 1 cut(s) 2222
MspI CCGG 2 cut(s) 35, 1431
MspR9I CCNGG 2 cut(s) 616, 1950
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 3 cut(s) 453, 1867, 2245
MvaI CCWGG 2 cut(s) 616, 1950
MvnI CGCG 4 cut(s) 193, 195, 263, 536
MwoI GCNNNNNNNGC 6 cut(s) 93, 255, 1295, 1847, 1979, 2129
NaeI GCCGGC 1 cut(s) 1432
NarI GGCGCC 1 cut(s) 99
NdeI CATATG 3 cut(s) 798, 1919, 2307
NdeII GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
NgoMIV GCCGGC 1 cut(s) 1430
NheI GCTAGC 2 cut(s) 1442, 2033
NlaIII CATG 8 cut(s) 166, 1250, 1337, 1609, 1683, 1777, 2108, 2123
NlaIV GGNNCC 4 cut(s) 74, 87, 100, 1899
NmeAIII GCCGAG 2 cut(s) 493, 499
NmuCI GTSAC 1 cut(s) 2197
NsiI ATGCAT 1 cut(s) 1660
NspI RCATGY 2 cut(s) 1683, 1777
OliI CACNNNNGTG 1 cut(s) 194
PaeI GCATGC 1 cut(s) 1683
PaqCI CACCTGC 2 cut(s) 511, 1877
PceI AGGCCT 1 cut(s) 776
PctI GAATGC 3 cut(s) 453, 1867, 2245
PdiI GCCGGC 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 7 cut(s) 620, 782, 811, 1514, 1553, 1627, 2302
PleI GAGTC 5 cut(s) 635, 954, 1311, 1813, 2002
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 5 cut(s) 635, 954, 1311, 1813, 2002
PpuMI RGGWCCY 1 cut(s) 2086
Psp124BI GAGCTC 2 cut(s) 23, 2134
Psp5II RGGWCCY 1 cut(s) 2086
Psp6I CCWGG 2 cut(s) 614, 1948
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 2 cut(s) 614, 1948
PspN4I GGNNCC 4 cut(s) 74, 87, 100, 1899
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 7 cut(s) 85, 86, 465, 1410, 1832, 2071, 2086
PspPPI RGGWCCY 1 cut(s) 2086
PstI CTGCAG 4 cut(s) 369, 727, 1294, 1855
PstNI CAGNNNCTG 2 cut(s) 1477, 1973
PsuI RGATCY 4 cut(s) 1562, 1696, 1717, 1897
RsaI GTAC 4 cut(s) 74, 211, 539, 1058
RsaNI GTAC 4 cut(s) 73, 210, 538, 1057
RseI CAYNNNNRTG 3 cut(s) 194, 1338, 1918
SacI GAGCTC 2 cut(s) 23, 2134
Sau3AI GATC 8 cut(s) 186, 500, 1223, 1562, 1696, 1717, 1747, 1897
Sau96I GGNCC 7 cut(s) 85, 86, 465, 1410, 1832, 2071, 2086
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 5 cut(s) 635, 955, 1312, 1814, 2003
ScrFI CCNGG 2 cut(s) 616, 1950
SduI GDGCHC 5 cut(s) 11, 23, 89, 246, 2134
SfaNI GCATC 8 cut(s) 477, 1030, 1144, 1264, 1600, 1667, 1960, 1991
SfcI CTRYAG 6 cut(s) 365, 545, 723, 1290, 1851, 2125
SfoI GGCGCC 1 cut(s) 100
SinI GGWCC 3 cut(s) 1832, 2071, 2086
SmiMI CAYNNNNRTG 3 cut(s) 194, 1338, 1918
SmlI CTYRAG 3 cut(s) 1394, 1435, 2222
SmoI CTYRAG 3 cut(s) 1394, 1435, 2222
SphI GCATGC 1 cut(s) 1683
SseBI AGGCCT 1 cut(s) 776
SspDI GGCGCC 1 cut(s) 98
SspI AATATT 4 cut(s) 223, 235, 412, 570
SspMI CTAG 6 cut(s) 444, 1299, 1443, 1893, 2034, 2313
SstI GAGCTC 2 cut(s) 23, 2134
StuI AGGCCT 1 cut(s) 776
StyD4I CCNGG 2 cut(s) 614, 1948
StyI CCWWGG 2 cut(s) 81, 1413
TaaI ACNGT 6 cut(s) 214, 241, 549, 680, 1232, 2197
TaiI ACGT 2 cut(s) 592, 977
TaqI TCGA 5 cut(s) 17, 132, 335, 461, 1826
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 7 cut(s) 620, 782, 811, 1514, 1553, 1627, 2302
TscAI CASTG 4 cut(s) 554, 685, 1284, 1992
TseFI GTSAC 1 cut(s) 2197
Tsp45I GTSAC 1 cut(s) 2197
TspDTI ATGAA 6 cut(s) 510, 839, 849, 1080, 1506, 2294
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 4 cut(s) 554, 685, 1284, 1992
Vha464I CTTAAG 1 cut(s) 2222
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 3 cut(s) 1832, 2071, 2086
XapI RAATTY 3 cut(s) 890, 1547, 2170
XbaI TCTAGA 1 cut(s) 2312
XceI RCATGY 2 cut(s) 1683, 1777
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 6 cut(s) 444, 1299, 1443, 1893, 2034, 2313
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.