MD09G1103500.v1.1

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011587
Physical Location & Seq
Reverse (-)
7576536 .. 7580538
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1103500.v1.1.491

Sequence Viewer

Length: 2691 bp
ATGGACGGGATGGAAATGGGAAGAGGCAGCTATGCGGTTGTTTTTGTGATTCTGCTTTGCGCGTTGGTGATAGGGGCGTCGGAGGGTGTGAGGAGGACGAGAGAAACATTTTTCGGCAATGGCGGCGATTGGCTCTCCGAGGAGATAGACGAAGACATGGCAGAGCAGGCATGGATCCATTGCAGGAAAGAACTGATAGATGAGAAAGATGCTATCGAAAAGTTTAACTTGTACATTCCACAGGAAGCAGCCGCTGACTCTGAAACAACGTTCTTAACGAAAAGAAACATACACCATGCAATTTTAGTTCTTCCTCATCATGTGAAAAAAACACTTTTTGATTGCTTAAGAAAGAAAGGGCTTCTGCTTCATGCTTCTGAAGAAAAGGCTACCTCGAAAAATTGGTTCATCAGCTGCTTAGGGTGGCTTTTAGGCTGGCCGGATGCTCCTAGAAGACATTTGGCCAGCGATTCACGTAAGCCAACAACGCCAAGTCCTTCTCCTTCTCCACAACCCGGGCATGCTCCAGTTCTAGCTCCCGGCCCTGCAGCTAGGTTGCCTAGATATCAAGCACCTTCACCTTCACCTTCATCCTCTAAGAAAACTCATACTACTTTTCACGCTAGTCCTAATCCCGCATCAAAGGATCCCTCGTCGAAAAAGAAAAATCCACCATCAAAGGATGCGAAACCCTCACCAAAAAAGAAAAATCCACCAGCAAAGGATCCCTCATTTGGTAAAAAAAAGGCGCCACAAAAGGATAACAGCAATGTGATTAAACTAATTGCTATTGCTGGAGCTGCAGTTGTAATATTTATCATTGTTGCACTTCTCTGTTTCTGCTGTATTAAGAAAAGGAGAAGCCGAAAAATTGGTCCAAGAAATGGACCAAATGATGATAGGCCTCTTCTCACAATATCCGCTGGTTCTTCTCAGAAGTCTACTAGTTTAGGAAATTCCAGTAACAAAGAGTTCGGCACTAGTGCAAATTTTGCAAACAATATGGCCCTGAAAAAAGAAAATGAATCTGCACCTGGTGATGCAACTACATCAGAAGCCGTGGCAGGAGGGTCAGTTCCTCCCCTAAAACCTCCCCCTGGAAGACAAGCTCCTCCACCACCTGAACCACCAGGTCCACCACCACCTCCTCCTCCTCGTCCTAAGGCCCCACCACCTCCGAAAGTTGCACCTCCACCGAAACCATTGCCTGGTAAGAGCAACCGTCCGCCTATAGGACTTCATCGCAAAGCAAACAGTGGAGGTAGTGGTGATATGGATGCTGAGGGATCTCAAAAGACCAAGCTAAAGCCATTCTTTTGGGATAAGGTTAATACCAATTCGGATCAGTCAATGGTCTGGCATGAGATCAGTGCAGGATCATTCCAATTCAATGAGGAGCAGATAGAGTCGCTATTTGGTTATAACACTGCGGATAAAGCCAAAAATGATCGCAGGAAGGAAGCATCAGCCTTAGAACCTGCAGTCCAGTATATTCAAATTATCGACAGAAAGAAAGCACAAAATCTTGCAATTCTTCTACGAGCATTGAATCTGACAGCGGAAGAAGTTGCTGATGCCCTCCGAGAGGGTAATGAGCTTCCTGTGGAGTTCCTCCAAAACTTGTTGAGGATGGCACCAACTGCGGAAGAAGAATTGAAGCTTAGGTTATTCACAGGGGATACTGCTCAACTTGGTCACGCAGAGCGGTTCCTCAAAGCCATGGTCGACATACCATTTGCTTTTAAGCGCATGGAGTCACTGTTGTTTATGTGTTCTCTGCCAGAGGAGGTTTCTACCACTAAAGAGTCCTTCATAACTTTAGAGGTTGCTTGCAACAAACTCAGAAAGAGCAGGCTATTCCTAAAACTTTTAGAAGCGGTTCTCAAAACTGGAAACCGTATGAATGATGGCACCTACCGTGGTGGTGCGCAGGCATTTAGGCTTGATACGCTTTTGAAACTCGCTGATGTAAAAGGCACTGATGGCAAAACCACACTCTTGCACTTTGTAGTTCAAGAGATCATGCGTTCTGAGGGTCGTAGAGCTATTCGCACAGCCAGAGAAAGCCGGAGCATGTCCAACTTGAGTACAGAGGACATAACTGAGGATGTCAATGAGGAATCAGAGGAGCACTTGTGTAACCTCGGTCTTCAGGTTGTCTCAGGTTTAAGCGATGAACTTGAAGATGTGAAAAAGGCTGCACTTGTAGATGCTGATGGCCTAACGTCGACAGTTGCAAATCTTGGCCAGTCCTTAATAAAAAATCGAGATTTTATAAATGTTGAGATGAAGAATATGGATGAAGATAGTGAATTCCAACGCACACTAGCCAGTTTTGTGGAGCGGGCGGAATCTGATATTACATGGCTGTTGGAAGAAGAAAAGAGGATAACTGCTCTTGTGAAGAGCACAGCGGATTACTTCCACGGGAACGCAGGGAGGGATGAAGGCATTCGCTTGTTTGCCATTGTACGAGATTTCTTGGTAATTCTAAACAAGGTATGCACAGAGGTCCGAAATTCGACAATGAAGGCGGCGAAGAATGCCAAGAAAGAGGCTCAGGCCCCAACGGCGACAGAAACAACTCCAGAAAATCGTCAAACACCACCTACAGATATGCGTCAACGACTATTTCCAGCAATTGCAGAACGGCGGGTCGAGGAGGGTTTCAGTTCAGATGATGAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04320 map04320
Pfam Domains
Protein Families
FH2

Protein Analysis

897

Amino Acids

98.56

Weight (kDa)

8.8

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 430 - 829 1.4e-101 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1422, 2276
Acc16I TGCGCA 1 cut(s) 1929
Acc36I ACCTGC 1 cut(s) 1486
AccB1I GGYRCC 3 cut(s) 748, 1633, 1910
AccB7I CCANNNNNTGG 2 cut(s) 884, 1208
AccBSI CCGCTC 2 cut(s) 1705, 2344
AccI GTMKAC 3 cut(s) 941, 1725, 2228
AccII CGCG 1 cut(s) 62
AclI AACGTT 1 cut(s) 269
AclWI GGATC 9 cut(s) 169, 182, 641, 654, 719, 732, 1294, 1350, 1384
AcoI YGGCCR 3 cut(s) 437, 462, 2245
AcsI RAATTY 4 cut(s) 955, 988, 2312, 2518
AcuI CTGAAG 2 cut(s) 399, 2135
AcyI GRCGYC 2 cut(s) 77, 749
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 3 cut(s) 233, 2089, 2472
AfiI CCNNNNNNNGG 7 cut(s) 515, 734, 884, 1097, 1208, 1232, 1585
AflII CTTAAG 1 cut(s) 346
AgsI TTSAA 7 cut(s) 1390, 1496, 1549, 1657, 1957, 2015, 2183
AhlI ACTAGT 2 cut(s) 944, 980
AjnI CCWGG 4 cut(s) 1033, 1096, 1129, 1207
AloI GAACNNNNNNTCC 4 cut(s) 1059, 1091, 1467, 1499
Alw21I GWGCWC 2 cut(s) 2133, 2411
Alw26I GTCTC 2 cut(s) 2164, 2678
AlwI GGATC 9 cut(s) 169, 182, 641, 654, 719, 732, 1294, 1350, 1384
AlwNI CAGNNNCTG 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 9 cut(s) 437, 462, 541, 902, 1005, 1164, 2218, 2245, 2562
ApeKI GCWGC 6 cut(s) 27, 248, 414, 548, 800, 2198
ApoI RAATTY 4 cut(s) 955, 988, 2312, 2518
ArsI GACNNNNNNTTYG 6 cut(s) 859, 891, 1707, 1718, 1739, 1750
Asp700I GAANNNNTTC 2 cut(s) 1878, 2451
AspLEI GCGC 4 cut(s) 62, 751, 1749, 1930
AspS9I GGNCC 8 cut(s) 542, 875, 887, 1006, 1133, 1165, 2512, 2563
AsuC2I CCSGG 3 cut(s) 516, 517, 540
AsuHPI GGTGA 6 cut(s) 79, 570, 576, 687, 1049, 1280
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 4 cut(s) 875, 887, 1133, 2512
AxyI CCTNAGG 1 cut(s) 1161
BalI TGGCCA 2 cut(s) 464, 2247
BamHI GGATCC 3 cut(s) 174, 646, 724
BanI GGYRCC 3 cut(s) 748, 1633, 1910
BbsI GAAGAC 4 cut(s) 159, 460, 1108, 2141
Bbv12I GWGCWC 2 cut(s) 2133, 2411
BbvCI CCTCAGC 1 cut(s) 1281
BbvI GCAGC 6 cut(s) 39, 260, 401, 560, 787, 2185
BccI CCATC 6 cut(s) 4, 682, 1624, 1901, 1976, 2210
BceAI ACGGC 3 cut(s) 1043, 2586, 2666
BcgI CGANNNNNNTGC 2 cut(s) 1186, 1220
BciT130I CCWGG 4 cut(s) 1035, 1098, 1131, 1209
BciVI GTATCC 1 cut(s) 1672
BcnI CCSGG 3 cut(s) 516, 517, 540
BcoDI GTCTC 2 cut(s) 2164, 2678
BcuI ACTAGT 2 cut(s) 944, 980
BfaI CTAG 9 cut(s) 450, 533, 552, 561, 624, 945, 981, 2327, 2689
BfmI CTRYAG 5 cut(s) 546, 801, 1230, 1479, 2610
BfoI RGCGCY 1 cut(s) 752
BfrI CTTAAG 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 1486
BfuI GTATCC 1 cut(s) 1672
BglI GCCNNNNNGGC 1 cut(s) 2570
BisI GCNGC 9 cut(s) 28, 124, 249, 252, 415, 549, 801, 2199, 2535
BlsI GCNGC 9 cut(s) 29, 125, 250, 253, 416, 550, 802, 2200, 2536
Bme1390I CCNGG 7 cut(s) 516, 517, 540, 1035, 1098, 1131, 1209
Bme18I GGWCC 4 cut(s) 875, 887, 1133, 2512
BmeT110I CYCGRG 1 cut(s) 515
BmgT120I GGNCC 8 cut(s) 542, 875, 887, 1006, 1133, 1165, 2512, 2563
BmiI GGNNCC 9 cut(s) 176, 648, 726, 750, 1167, 1635, 1709, 1912, 2565
BmrFI CCNGG 7 cut(s) 516, 517, 540, 1035, 1098, 1131, 1209
BmsI GCATC 9 cut(s) 199, 433, 647, 673, 1030, 1267, 1472, 1564, 2200
BpiI GAAGAC 4 cut(s) 159, 460, 1108, 2141
BpmI CTGGAG 3 cut(s) 510, 816, 2571
Bpu10I CCTNAGC 4 cut(s) 418, 1281, 1661, 2559
BpuEI CTTGAG 1 cut(s) 2104
BpuMI CCSGG 3 cut(s) 516, 517, 540
BsaAI YACGTR 1 cut(s) 476
BsaHI GRCGYC 2 cut(s) 77, 749
BsaI GGTCTC 1 cut(s) 2678
BsaJI CCNNGG 8 cut(s) 138, 515, 1059, 1096, 1719, 1918, 2143, 2425
BsaXI ACNNNNNCTCC 8 cut(s) 789, 819, 1059, 1089, 1745, 1775, 2120, 2150
Bsc4I CCNNNNNNNGG 7 cut(s) 515, 734, 884, 1097, 1208, 1232, 1585
Bse1I ACTGG 6 cut(s) 527, 960, 1486, 1894, 2248, 2331
Bse21I CCTNAGG 1 cut(s) 1161
Bse3DI GCAATG 4 cut(s) 124, 178, 775, 1202
BseBI CCWGG 4 cut(s) 1035, 1098, 1131, 1209
BseDI CCNNGG 8 cut(s) 138, 515, 1059, 1096, 1719, 1918, 2143, 2425
BseGI GGATG 9 cut(s) 15, 448, 590, 688, 1282, 1635, 2113, 2305, 2449
BseLI CCNNNNNNNGG 7 cut(s) 515, 734, 884, 1097, 1208, 1232, 1585
BseMI GCAATG 4 cut(s) 124, 178, 775, 1202
BseMII CTCAG 7 cut(s) 947, 1272, 1855, 2022, 2094, 2175, 2573
BseNI ACTGG 6 cut(s) 527, 960, 1486, 1894, 2248, 2331
BseXI GCAGC 6 cut(s) 39, 260, 401, 560, 787, 2185
BsgI GTGCAG 3 cut(s) 1014, 1392, 2184
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 9 cut(s) 439, 464, 543, 904, 1007, 1166, 2220, 2247, 2564
BshNI GGYRCC 3 cut(s) 748, 1633, 1910
BsiHKAI GWGCWC 2 cut(s) 2133, 2411
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 4 cut(s) 440, 516, 540, 2068
BslI CCNNNNNNNGG 7 cut(s) 515, 734, 884, 1097, 1208, 1232, 1585
BsmAI GTCTC 2 cut(s) 2164, 2678
BsmI GAATGC 2 cut(s) 2451, 2548
BsnI GGCC 9 cut(s) 439, 464, 543, 904, 1007, 1166, 2220, 2247, 2564
Bso31I GGTCTC 1 cut(s) 2678
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 2133, 2411
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
Bsp19I CCATGG 1 cut(s) 1719
BspANI GGCC 9 cut(s) 439, 464, 543, 904, 1007, 1166, 2220, 2247, 2564
BspCNI CTCAG 7 cut(s) 946, 1273, 1854, 2023, 2095, 2174, 2572
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 9 cut(s) 176, 648, 726, 750, 1167, 1635, 1709, 1912, 2565
BspMAI CTGCAG 3 cut(s) 550, 805, 1483
BspMI ACCTGC 1 cut(s) 1486
BspPI GGATC 9 cut(s) 169, 182, 641, 654, 719, 732, 1294, 1350, 1384
BspQI GCTCTTC 1 cut(s) 2399
BspT107I GGYRCC 3 cut(s) 748, 1633, 1910
BspTI CTTAAG 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 2678
BsrBI CCGCTC 2 cut(s) 1705, 2344
BsrDI GCAATG 4 cut(s) 124, 178, 775, 1202
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 6 cut(s) 527, 960, 1486, 1894, 2248, 2331
BssECI CCNNGG 8 cut(s) 138, 515, 1059, 1096, 1719, 1918, 2143, 2425
BssMI GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
BssNI GRCGYC 2 cut(s) 77, 749
BssT1I CCWWGG 1 cut(s) 1719
Bst2UI CCWGG 4 cut(s) 1035, 1098, 1131, 1209
Bst4CI ACNGT 6 cut(s) 1223, 1256, 1761, 1898, 1919, 2233
Bst6I CTCTTC 3 cut(s) 16, 912, 2399
BstACI GRCGYC 2 cut(s) 77, 749
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 2 cut(s) 992, 1640
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 476
BstC8I GCNNGC 8 cut(s) 168, 437, 466, 522, 1831, 1853, 1932, 2346
BstDSI CCRYGG 4 cut(s) 1059, 1719, 1918, 2425
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 9 cut(s) 15, 448, 590, 688, 1282, 1635, 2113, 2305, 2449
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 752
BstHHI GCGC 4 cut(s) 62, 751, 1749, 1930
BstKTI GATC 9 cut(s) 177, 649, 727, 1289, 1345, 1368, 1379, 1450, 2022
BstMAI GTCTC 2 cut(s) 2164, 2678
BstMBI GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
BstNI CCWGG 4 cut(s) 1035, 1098, 1131, 1209
BstNSI RCATGY 2 cut(s) 524, 2077
BstSCI CCNGG 7 cut(s) 514, 515, 538, 1033, 1096, 1129, 1207
BstSFI CTRYAG 5 cut(s) 546, 801, 1230, 1479, 2610
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 6 cut(s) 39, 260, 401, 560, 787, 2185
BstV2I GAAGAC 4 cut(s) 159, 460, 1108, 2141
BstX2I RGATCY 4 cut(s) 174, 646, 724, 1286
BstXI CCANNNNNNTGG 2 cut(s) 1317, 2338
BstYI RGATCY 4 cut(s) 174, 646, 724, 1286
Bsu36I CCTNAGG 1 cut(s) 1161
BsuI GTATCC 1 cut(s) 1672
BsuRI GGCC 9 cut(s) 439, 464, 543, 904, 1007, 1166, 2220, 2247, 2564
BtgI CCRYGG 4 cut(s) 1059, 1719, 1918, 2425
BtgZI GCGATG 2 cut(s) 1226, 2187
BtsCI GGATG 9 cut(s) 15, 448, 590, 688, 1282, 1635, 2113, 2305, 2449
BtsI GCAGTG 1 cut(s) 1425
BtsIMutI CAGTG 5 cut(s) 1261, 1375, 1425, 1757, 1977
BveI ACCTGC 1 cut(s) 1486
Cac8I GCNNGC 8 cut(s) 168, 437, 466, 522, 1831, 1853, 1932, 2346
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 4 cut(s) 62, 751, 1749, 1930
Cfr13I GGNCC 8 cut(s) 542, 875, 887, 1006, 1133, 1165, 2512, 2563
Cfr9I CCCGGG 1 cut(s) 515
CseI GACGC 2 cut(s) 66, 2609
CsiI ACCWGGT 2 cut(s) 1033, 1129
Csp6I GTAC 3 cut(s) 232, 2088, 2471
CviQI GTAC 3 cut(s) 232, 2088, 2471
DinI GGCGCC 1 cut(s) 750
DpnI GATC 9 cut(s) 176, 648, 726, 1288, 1344, 1367, 1378, 1449, 2021
DpnII GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
DraIII CACNNNGTG 1 cut(s) 1037
EaeI YGGCCR 3 cut(s) 437, 462, 2245
Eam1104I CTCTTC 3 cut(s) 16, 912, 2399
EarI CTCTTC 3 cut(s) 16, 912, 2399
EciI GGCGGA 2 cut(s) 1215, 2363
Eco130I CCWWGG 1 cut(s) 1719
Eco147I AGGCCT 1 cut(s) 904
Eco31I GGTCTC 1 cut(s) 2678
Eco32I GATATC 1 cut(s) 566
Eco47I GGWCC 4 cut(s) 875, 887, 1133, 2512
Eco57I CTGAAG 2 cut(s) 399, 2135
Eco81I CCTNAGG 1 cut(s) 1161
Eco88I CYCGRG 1 cut(s) 515
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 2 cut(s) 1165, 2563
EcoRI GAATTC 1 cut(s) 2312
EcoRII CCWGG 4 cut(s) 1033, 1096, 1129, 1207
EcoRV GATATC 1 cut(s) 566
EcoT14I CCWWGG 1 cut(s) 1719
EgeI GGCGCC 1 cut(s) 750
EheI GGCGCC 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 1719
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 1298, 1330
FauI CCCGC 3 cut(s) 643, 2337, 2646
FblI GTMKAC 3 cut(s) 941, 1725, 2228
Fnu4HI GCNGC 9 cut(s) 28, 124, 249, 252, 415, 549, 801, 2199, 2535
FokI GGATG 9 cut(s) 22, 455, 577, 695, 1289, 1642, 2120, 2312, 2456
Fsp4HI GCNGC 9 cut(s) 28, 124, 249, 252, 415, 549, 801, 2199, 2535
FspBI CTAG 9 cut(s) 450, 533, 552, 561, 624, 945, 981, 2327, 2689
FspI TGCGCA 1 cut(s) 1929
GlaI GCGC 4 cut(s) 61, 750, 1748, 1929
GluI GCNGC 9 cut(s) 28, 124, 249, 252, 415, 549, 801, 2199, 2535
GsuI CTGGAG 3 cut(s) 510, 816, 2571
HaeII RGCGCY 1 cut(s) 752
HaeIII GGCC 9 cut(s) 439, 464, 543, 904, 1007, 1166, 2220, 2247, 2564
HapII CCGG 4 cut(s) 440, 516, 540, 2068
HgaI GACGC 2 cut(s) 66, 2609
HhaI GCGC 4 cut(s) 62, 751, 1749, 1930
Hin1I GRCGYC 2 cut(s) 77, 749
Hin6I GCGC 4 cut(s) 60, 749, 1747, 1928
HinP1I GCGC 4 cut(s) 60, 749, 1747, 1928
HincII GTYRAC 3 cut(s) 1726, 2229, 2624
HindII GTYRAC 3 cut(s) 1726, 2229, 2624
HindIII AAGCTT 1 cut(s) 1658
HpaII CCGG 4 cut(s) 440, 516, 540, 2068
HphI GGTGA 6 cut(s) 79, 570, 576, 687, 1049, 1280
Hpy166II GTNNAC 5 cut(s) 942, 1136, 1726, 2229, 2624
Hpy188III TCNNGA 3 cut(s) 2015, 2267, 2588
Hpy8I GTNNAC 5 cut(s) 942, 1136, 1726, 2229, 2624
Hpy99I CGWCG 3 cut(s) 82, 658, 2230
HpyAV CCTTC 9 cut(s) 507, 513, 585, 591, 597, 1450, 1819, 2441, 2524
HpyCH4III ACNGT 6 cut(s) 1223, 1256, 1761, 1898, 1919, 2233
HpyCH4IV ACGT 3 cut(s) 269, 475, 2225
HpySE526I ACGT 3 cut(s) 269, 475, 2225
Hsp92I GRCGYC 2 cut(s) 77, 749
HspAI GCGC 4 cut(s) 60, 749, 1747, 1928
KasI GGCGCC 1 cut(s) 748
Kzo9I GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
LguI GCTCTTC 1 cut(s) 2399
LmnI GCTCC 9 cut(s) 451, 529, 541, 797, 1114, 1396, 2070, 2128, 2341
Lsp1109I GCAGC 6 cut(s) 39, 260, 401, 560, 787, 2185
LweI GCATC 9 cut(s) 199, 433, 647, 673, 1030, 1267, 1472, 1564, 2200
MabI ACCWGGT 2 cut(s) 1033, 1129
MaeI CTAG 9 cut(s) 450, 533, 552, 561, 624, 945, 981, 2327, 2689
MaeII ACGT 3 cut(s) 269, 475, 2225
MaeIII GTNAC 4 cut(s) 962, 1694, 1755, 2138
MalI GATC 9 cut(s) 176, 648, 726, 1288, 1344, 1367, 1378, 1449, 2021
MbiI CCGCTC 2 cut(s) 1705, 2344
MboI GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
MfeI CAATTG 1 cut(s) 2640
MflI RGATCY 4 cut(s) 174, 646, 724, 1286
MhlI GDGCHC 2 cut(s) 2133, 2411
MlsI TGGCCA 2 cut(s) 464, 2247
MluNI TGGCCA 2 cut(s) 464, 2247
Mly113I GGCGCC 1 cut(s) 749
MlyI GAGTC 4 cut(s) 251, 1415, 1763, 1814
MmeI TCCRAC 4 cut(s) 60, 2103, 2341, 2352
Mox20I TGGCCA 2 cut(s) 464, 2247
MroXI GAANNNNTTC 2 cut(s) 1878, 2451
MscI TGGCCA 2 cut(s) 464, 2247
MseI TTAA 9 cut(s) 225, 275, 347, 777, 849, 1329, 1743, 2168, 2255
Msp20I TGGCCA 2 cut(s) 464, 2247
MspA1I CMGCKG 5 cut(s) 254, 414, 923, 1559, 2414
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 4 cut(s) 440, 516, 540, 2068
MspR9I CCNGG 7 cut(s) 516, 517, 540, 1035, 1098, 1131, 1209
MunI CAATTG 1 cut(s) 2640
Mva1269I GAATGC 2 cut(s) 2451, 2548
MvaI CCWGG 4 cut(s) 1035, 1098, 1131, 1209
MvnI CGCG 1 cut(s) 62
NarI GGCGCC 1 cut(s) 749
NciI CCSGG 3 cut(s) 516, 517, 540
NcoI CCATGG 1 cut(s) 1719
NdeII GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
NlaIV GGNNCC 9 cut(s) 176, 648, 726, 750, 1167, 1635, 1709, 1912, 2565
NmuCI GTSAC 2 cut(s) 1694, 1755
NsbI TGCGCA 1 cut(s) 1929
NspI RCATGY 2 cut(s) 524, 2077
PaeI GCATGC 1 cut(s) 524
PceI AGGCCT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 2399
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PctI GAATGC 2 cut(s) 2451, 2548
PdmI GAANNNNTTC 2 cut(s) 1878, 2451
PfeI GAWTC 6 cut(s) 49, 470, 1025, 1549, 2120, 2351
PflMI CCANNNNNTGG 2 cut(s) 884, 1208
PkrI GCNGC 9 cut(s) 29, 125, 250, 253, 416, 550, 802, 2200, 2536
PleI GAGTC 4 cut(s) 251, 1414, 1762, 1813
PluTI GGCGCC 1 cut(s) 752
PpsI GAGTC 4 cut(s) 251, 1414, 1762, 1813
Ppu21I YACGTR 1 cut(s) 476
PsiI TTATAA 2 cut(s) 1422, 2276
Psp1406I AACGTT 1 cut(s) 269
Psp6I CCWGG 4 cut(s) 1033, 1096, 1129, 1207
PspGI CCWGG 4 cut(s) 1033, 1096, 1129, 1207
PspN4I GGNNCC 9 cut(s) 176, 648, 726, 750, 1167, 1635, 1709, 1912, 2565
PspPI GGNCC 8 cut(s) 542, 875, 887, 1006, 1133, 1165, 2512, 2563
PstI CTGCAG 3 cut(s) 550, 805, 1483
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 4 cut(s) 174, 646, 724, 1286
PvuII CAGCTG 1 cut(s) 414
RsaI GTAC 3 cut(s) 233, 2089, 2472
RsaNI GTAC 3 cut(s) 232, 2088, 2471
SalI GTCGAC 2 cut(s) 1724, 2227
SapI GCTCTTC 1 cut(s) 2399
SaqAI TTAA 9 cut(s) 225, 275, 347, 777, 849, 1329, 1743, 2168, 2255
SatI GCNGC 9 cut(s) 28, 124, 249, 252, 415, 549, 801, 2199, 2535
Sau3AI GATC 9 cut(s) 174, 646, 724, 1286, 1342, 1365, 1376, 1447, 2019
Sau96I GGNCC 8 cut(s) 542, 875, 887, 1006, 1133, 1165, 2512, 2563
SchI GAGTC 4 cut(s) 251, 1415, 1763, 1814
ScrFI CCNGG 7 cut(s) 516, 517, 540, 1035, 1098, 1131, 1209
SduI GDGCHC 2 cut(s) 2133, 2411
SexAI ACCWGGT 2 cut(s) 1033, 1129
SfaNI GCATC 9 cut(s) 199, 433, 647, 673, 1030, 1267, 1472, 1564, 2200
SfcI CTRYAG 5 cut(s) 546, 801, 1230, 1479, 2610
SfoI GGCGCC 1 cut(s) 750
SinI GGWCC 4 cut(s) 875, 887, 1133, 2512
SmaI CCCGGG 1 cut(s) 517
SmlI CTYRAG 2 cut(s) 346, 2083
SmoI CTYRAG 2 cut(s) 346, 2083
SpeI ACTAGT 2 cut(s) 944, 980
SphI GCATGC 1 cut(s) 524
SseBI AGGCCT 1 cut(s) 904
SspDI GGCGCC 1 cut(s) 748
SspI AATATT 1 cut(s) 813
SspMI CTAG 9 cut(s) 450, 533, 552, 561, 624, 945, 981, 2327, 2689
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 7 cut(s) 514, 515, 538, 1033, 1096, 1129, 1207
StyI CCWWGG 1 cut(s) 1719
TaaI ACNGT 6 cut(s) 1223, 1256, 1761, 1898, 1919, 2233
TaiI ACGT 3 cut(s) 272, 478, 2228
TaqI TCGA 9 cut(s) 216, 395, 656, 1503, 1725, 2228, 2266, 2522, 2658
TaqII GACCGA 1 cut(s) 2135
TatI WGTACW 2 cut(s) 231, 2087
TauI GCSGC 3 cut(s) 126, 254, 2537
TfiI GAWTC 6 cut(s) 49, 470, 1025, 1549, 2120, 2351
Tru1I TTAA 9 cut(s) 225, 275, 347, 777, 849, 1329, 1743, 2168, 2255
Tru9I TTAA 9 cut(s) 225, 275, 347, 777, 849, 1329, 1743, 2168, 2255
TscAI CASTG 5 cut(s) 1261, 1375, 1432, 1764, 1984
TseFI GTSAC 2 cut(s) 1694, 1755
TseI GCWGC 6 cut(s) 27, 248, 414, 548, 800, 2198
Tsp45I GTSAC 2 cut(s) 1694, 1755
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 5 cut(s) 1261, 1375, 1432, 1764, 1984
Van91I CCANNNNNTGG 2 cut(s) 884, 1208
Vha464I CTTAAG 1 cut(s) 346
VpaK11BI GGWCC 4 cut(s) 875, 887, 1133, 2512
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 4 cut(s) 955, 988, 2312, 2518
XceI RCATGY 2 cut(s) 524, 2077
XmaI CCCGGG 1 cut(s) 515
XmiI GTMKAC 3 cut(s) 941, 1725, 2228
XmnI GAANNNNTTC 2 cut(s) 1878, 2451
XspI CTAG 9 cut(s) 450, 533, 552, 561, 624, 945, 981, 2327, 2689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.