Rw2G045370

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011587
Physical Location & Seq
Forward (+)
72286215 .. 72291672
5458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045370.1

Sequence Viewer

Length: 2973 bp
ATGGTGATTTGGGAAGGGATGCAAATGAGAAGAGCCAGTTATGCGGTTTTCGTGATTCTGCTCTGTGCATTGGTCATTGGGGCCTCAGAGGGCGTCAGGAGGACCAAAGAAACGTTTTATGGAGGTGATTGGGTTTCGCATGAGGAGGAGATGGATGAACATATGGCGGAGCAGGTATGGTACCATTGCAAGAAAGAATTAATAGGCGGGACAGATGGGGTGGAAGATCTTGATTTGTATATTCCACAGGAAGCAGCCAGTGACGAAACAGTCTTCTTTTCAAAAAGCAACATACACGATGCGATTTTAGTTCTTCCTCCTGATGTGAAACAAACACTTGTTGATTGCTTAAGAAAGAAAGGGATTGTGTTTCATGCTCCTAGCAGAAGGGATAGCGGCAACAACTGGTTGATCAACTACTTTGAGTGGCTTTTAAGTTCGGATATTGCTCCTAGAAGATATTTGGCCAGTGGTTCCCCCAAGTCAAAAGCACCAGCCGCTTCTCCATCTCTAGCTCCAGGTCCTGCTTCTAAATTGTCTAGCGGTCGAGCACCTCCACGTTCAGTTCTATCTCCAAGCCCTACAGATGAGTCAGATAACAATGAAGCACCAGCACCTTCACCTTCACCTCCTAAACAAAGTGGGAAGGGAAGTCCACCTCCCAAACCAAGTGGGAAAGTAGCTGCTTCAAGTCCACCAGCAAAACCGGCCAAAACAAAAAGTCCACCAGCAAAACCAGCCAAAACAAATGGTCCACCATCAAAGGATGGCAGTATGAGTATGATCAGAATAATTGGTATTGCTATTGCTGCAGTAGTGATAATTTTGCTTGTTGTACTGCCGATCTGCTGCTGCTGTCTAAAGAAAAGAAGAAGGAGAAGAAAAGTTAATCCGAGAAGTGTACCAAAGGATGACAGGCCTCTTCTCTATATATCTTCTTCTGGTTCTTCACAAATATCCAATAGTTTAGGTTCAAGTACCAGAGAGTTCAACGCTGGCGGAAAGTTGGGAAACTTGCCTCTGAAAAATGAAAAAGGAAATGATTCTTCTGAGGCAAAGTCAGAAGGTACAGCAGGACCACCAAACCCTCCTGTACCACCTCCTCCAGGAAAAGCAGCTCCTCCGCCTCCTGGACCACCACCACCGCCTCCTCTTCGTCCTCGCCCGCCACCAGTACCTCCCAAAAATGCACCTAAACCAATGGCTGGTAAGACCCAAATTCCACCTAAAGGAAATCTCAAAGCAAACAGTGGCGATAATGCTGATGCTGGAAGTGAATCTGGAAAGACCAAGTTAAAACCATTCTTCTGGGATAAGGTTAATGCCAACCCTGATCAATCGATGGTCTGGCATGAGCTCAAAGAAGGATCATTCCAGTTCAATGAGGAGAAGATAGAGTCTCTATTTGGTTATACAAAGAGCCAAAATGAGCGCAAAAAGGTTTCACCATCGGAACCTGCAATCCAGTTTATCCAAATTATTGACAAAAAGAAAGCACAAAATCTATCAATTCTTCTGCGAGCATTGAATGTGACAACAGAAGAAGTTGCTGAGGCCCTCCGAGAAGGCAACGAGCTTCCTGTGGAGCTCCTCCAAACCCTGTTGAAGATGGCACCAACTTCAGAAGAGGAACTGAAGCTTAGGTTATACACAGGTGATCTTGCTCTACTAGGCCCTGCAGAGCGGTTCCTCAAAGCCATGGTTGAGATACCATTTGCTTTTAAGCGGATGGAGGCACTGCTCTTCATGTGTTCCTTCAATGAGGAGGTCTCTAACACTAAAGAATCCTTTGCAACTTTAGAGGTTGCTTGCAGCAAGCTCAGGAAGAGCAGGCTATTCCTAAAACTTTTGGAAGCCGTACTAAAAACTGGCAACCGGATGAATGACGGTACCTACCGTGGTGGTGCACAAGCATTTAGGCTTGATACTCTCTTGAAACTGGCTGATGTAAAAGGAACTGATGGCAAGACCACACTCTTGCACTTTGTCGTTCAAGAGATCATCCGTTATGAGGGCATGAAAGCTGCCCGCAGAGCCAGAGAATCAAACCTGAGCATGTCCAACTCCAGTATGAGTACAGAGAAGTTTGTTGAAGAGGTTGGTGAGGAATTAGCAGAGCACTATCGTAACCTTGGTCTTCAGGTTGTCTCAGGTTTAAGCGATGAACTTCAAGATGTGAAAAAGGCAGCAGTTGTAGATGCCGATAGCTTAACTGCAACAGTTTCAAATATTGGCACAAATCTAATAAAAGCTCGAGACTTTGTAAACACCGACTTGAAGAATAATCTGGATGAAGACAGCGAGTTCCATCGTGCACTAGCCAGTTTTCTGGAGCGTGCAGAAGGAGAAACTACATTTCTTCTAGAAGAAGAAAGGAGGATAACGACTCTTGTGAAGAGCACGGCAGATTACTTTCATGGGAACGCAATGAAGGATGAAGGCATTCGTTTGTTTGCCATTGTACGTGATTTTTTGGTAATACTAAATAAGGTATGTGCTGAGATTAGAATGCAAGCAATGAAGCAAGCGAAGACGAATTCCAGGAAAGAGGCTCTCGCACAAGCAAGTCCCAAGAAGGAGGCTCTCACACAAGCAAAATCAGAAAACCAGTCAGAAATGAATAAGGTACCCACAGAAGCTAGTTCAGAAAAGAAAGAGAAGGTTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGACTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTTCCACAGAAGCAAGTTCAGAAAAGAAAGAGAAGGCACCCACTGAAGCAAGTTCAGAAAATAAAGAGAAGGCTCCAACTGCAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACAGAAGCAAGTTCAGAAAAACCGTCAGAAATTCAACAGCAACATTCAGATATTCGTCGGCGACTATTTCCAGCAATTGCAGAACTGCGAATACAGGATGATTTCAGTTCAGATGATGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04320 map04320
Pfam Domains
Protein Families
FH2

Protein Analysis

990

Amino Acids

108.35

Weight (kDa)

8.74

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 428 - 826 3e-102 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
Acc36I ACCTGC 2 cut(s) 163, 1465
Acc65I GGTACC 3 cut(s) 180, 1889, 2626
AccB1I GGYRCC 5 cut(s) 180, 1612, 1889, 2626, 2770
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccBSI CCGCTC 1 cut(s) 1684
AclI AACGTT 1 cut(s) 113
AclWI GGATC 1 cut(s) 1375
AcoI YGGCCR 2 cut(s) 465, 708
AcsI RAATTY 3 cut(s) 1218, 2536, 2877
AcuI CTGAAG 6 cut(s) 1605, 1655, 2123, 2691, 2727, 2799
AcyI GRCGYC 1 cut(s) 93
AfiI CCNNNNNNNGG 5 cut(s) 1106, 1130, 1205, 1229, 2011
AflII CTTAAG 1 cut(s) 349
AjnI CCWGG 4 cut(s) 517, 1105, 1129, 2540
AloI GAACNNNNNNTCC 2 cut(s) 1446, 1478
Alw21I GWGCWC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
Alw26I GTCTC 5 cut(s) 1404, 1774, 2152, 2250, 2960
Alw44I GTGCAC 2 cut(s) 1905, 2313
AlwI GGATC 1 cut(s) 1375
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 2253
AoxI GGCC 6 cut(s) 81, 465, 708, 917, 1554, 1672
ApaLI GTGCAC 2 cut(s) 1905, 2313
ApoI RAATTY 3 cut(s) 1218, 2536, 2877
AseI ATTAAT 1 cut(s) 200
Asp700I GAANNNNTTC 2 cut(s) 1042, 2442
Asp718I GGTACC 3 cut(s) 180, 1889, 2626
AspLEI GCGC 1 cut(s) 1434
AspS9I GGNCC 8 cut(s) 81, 102, 521, 752, 1076, 1133, 1555, 1673
AsuHPI GGTGA 7 cut(s) 16, 137, 612, 618, 1437, 1667, 2114
AvaI CYCGRG 1 cut(s) 2253
AvaII GGWCC 5 cut(s) 102, 521, 752, 1076, 1133
BaeGI GKGCMC 2 cut(s) 1909, 2317
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 5 cut(s) 180, 1612, 1889, 2626, 2770
BanII GRGCYC 2 cut(s) 1359, 1590
BbsI GAAGAC 5 cut(s) 265, 2129, 2301, 2537, 2702
Bbv12I GWGCWC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
BbvCI CCTCAGC 1 cut(s) 1551
BceAI ACGGC 2 cut(s) 1841, 2418
BciT130I CCWGG 4 cut(s) 519, 1107, 1131, 2542
BclI TGATCA 3 cut(s) 411, 783, 1333
BcoDI GTCTC 5 cut(s) 1404, 1774, 2152, 2250, 2960
BfaI CTAG 8 cut(s) 381, 453, 512, 540, 1670, 2318, 2363, 2640
BfmI CTRYAG 4 cut(s) 582, 810, 1677, 2814
BfrI CTTAAG 1 cut(s) 349
BfuAI ACCTGC 2 cut(s) 163, 1465
BglII AGATCT 1 cut(s) 226
Bme1390I CCNGG 4 cut(s) 519, 1107, 1131, 2542
Bme18I GGWCC 5 cut(s) 102, 521, 752, 1076, 1133
BmeT110I CYCGRG 1 cut(s) 2253
BmgT120I GGNCC 8 cut(s) 81, 102, 521, 752, 1076, 1133, 1555, 1673
BmrFI CCNGG 4 cut(s) 519, 1107, 1131, 2542
BmsI GCATC 4 cut(s) 9, 289, 1255, 2188
BpiI GAAGAC 5 cut(s) 265, 2129, 2301, 2537, 2702
BplI GAGNNNNNCTC 2 cut(s) 1754, 1786
BpmI CTGGAG 4 cut(s) 501, 1089, 2050, 2351
Bpu10I CCTNAGC 4 cut(s) 1551, 1640, 1820, 2051
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 1 cut(s) 2465
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 1774
BsaJI CCNNGG 3 cut(s) 1698, 1897, 2131
BsaWI WCCGGW 1 cut(s) 1875
Bsc4I CCNNNNNNNGG 5 cut(s) 1106, 1130, 1205, 1229, 2011
Bse118I RCCGGY 1 cut(s) 706
Bse3DI GCAATG 3 cut(s) 184, 2433, 2523
BseBI CCWGG 4 cut(s) 519, 1107, 1131, 2542
BseCI ATCGAT 1 cut(s) 1340
BseDI CCNNGG 3 cut(s) 1698, 1897, 2131
BseLI CCNNNNNNNGG 5 cut(s) 1106, 1130, 1205, 1229, 2011
BseMI GCAATG 3 cut(s) 184, 2433, 2523
BseMII CTCAG 7 cut(s) 99, 1041, 1542, 1834, 2042, 2163, 2490
BseRI GAGGAG 8 cut(s) 158, 161, 1092, 1110, 1140, 1400, 1580, 1778
BseSI GKGCMC 2 cut(s) 1909, 2317
BsgI GTGCAG 1 cut(s) 2358
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 6 cut(s) 83, 467, 710, 919, 1556, 1674
BshNI GGYRCC 5 cut(s) 180, 1612, 1889, 2626, 2770
BshVI ATCGAT 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
BsiHKCI CYCGRG 1 cut(s) 2253
BsiSI CCGG 2 cut(s) 707, 1876
BslFI GGGAC 2 cut(s) 223, 2553
BslI CCNNNNNNNGG 5 cut(s) 1106, 1130, 1205, 1229, 2011
BsmAI GTCTC 5 cut(s) 1404, 1774, 2152, 2250, 2960
BsmFI GGGAC 2 cut(s) 223, 2553
BsmI GAATGC 2 cut(s) 2442, 2514
BsnI GGCC 6 cut(s) 83, 467, 710, 919, 1556, 1674
Bso31I GGTCTC 1 cut(s) 1774
BsoBI CYCGRG 1 cut(s) 2253
Bsp1286I GDGCHC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
Bsp143I GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
Bsp19I CCATGG 1 cut(s) 1698
BspANI GGCC 6 cut(s) 83, 467, 710, 919, 1556, 1674
BspCNI CTCAG 7 cut(s) 98, 1042, 1543, 1833, 2043, 2162, 2491
BspDI ATCGAT 1 cut(s) 1340
BspMAI CTGCAG 3 cut(s) 814, 1681, 2818
BspMI ACCTGC 2 cut(s) 163, 1465
BspPI GGATC 1 cut(s) 1375
BspQI GCTCTTC 4 cut(s) 25, 1748, 1820, 2390
BspT107I GGYRCC 5 cut(s) 180, 1612, 1889, 2626, 2770
BspTI CTTAAG 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 1774
BsrBI CCGCTC 1 cut(s) 1684
BsrDI GCAATG 3 cut(s) 184, 2433, 2523
BsrFI RCCGGY 1 cut(s) 706
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 3 cut(s) 1698, 1897, 2131
BssMI GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 1698, 2131
Bst2UI CCWGG 4 cut(s) 519, 1107, 1131, 2542
Bst4CI ACNGT 6 cut(s) 271, 1250, 1889, 1898, 2221, 2871
Bst6I CTCTTC 8 cut(s) 25, 927, 1158, 1620, 1748, 1820, 2088, 2390
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 349
BstBAI YACGTR 1 cut(s) 2465
BstDEI CTNAG 8 cut(s) 85, 1050, 1551, 1640, 1820, 2051, 2149, 2499
BstDSI CCRYGG 2 cut(s) 1698, 1897
BstENI CCTNNNNNAGG 1 cut(s) 1104
BstHHI GCGC 1 cut(s) 1434
BstKTI GATC 8 cut(s) 229, 414, 786, 846, 1336, 1370, 1660, 2001
BstMAI GTCTC 5 cut(s) 1404, 1774, 2152, 2250, 2960
BstMBI GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 7 cut(s) 41, 497, 707, 737, 809, 2033, 2813
BstNI CCWGG 4 cut(s) 519, 1107, 1131, 2542
BstNSI RCATGY 1 cut(s) 2059
BstSCI CCNGG 4 cut(s) 517, 1105, 1129, 2540
BstSFI CTRYAG 4 cut(s) 582, 810, 1677, 2814
BstSLI GKGCMC 2 cut(s) 1909, 2317
BstV2I GAAGAC 5 cut(s) 265, 2129, 2301, 2537, 2702
BstX2I RGATCY 1 cut(s) 226
BstXI CCANNNNNNTGG 2 cut(s) 1308, 2329
BstYI RGATCY 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 1340
BsuRI GGCC 6 cut(s) 83, 467, 710, 919, 1556, 1674
BsuTUI ATCGAT 1 cut(s) 1340
BtgI CCRYGG 2 cut(s) 1698, 1897
BtgZI GCGATG 1 cut(s) 2175
BtsI GCAGTG 1 cut(s) 1736
BtsIMutI CAGTG 7 cut(s) 265, 475, 1255, 1736, 2667, 2703, 2775
BveI ACCTGC 2 cut(s) 163, 1465
CaiI CAGNNNCTG 1 cut(s) 524
CfoI GCGC 1 cut(s) 1434
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 8 cut(s) 81, 102, 521, 752, 1076, 1133, 1555, 1673
ClaI ATCGAT 1 cut(s) 1340
CseI GACGC 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 8 cut(s) 140, 374, 1352, 1699, 1747, 2017, 2056, 2417
DdeI CTNAG 8 cut(s) 85, 1050, 1551, 1640, 1820, 2051, 2149, 2499
DpnI GATC 8 cut(s) 228, 413, 785, 845, 1335, 1369, 1659, 2000
DpnII GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 2 cut(s) 465, 708
Eam1104I CTCTTC 8 cut(s) 25, 927, 1158, 1620, 1748, 1820, 2088, 2390
EarI CTCTTC 8 cut(s) 25, 927, 1158, 1620, 1748, 1820, 2088, 2390
EciI GGCGGA 3 cut(s) 182, 1014, 1113
Ecl136II GAGCTC 2 cut(s) 1357, 1588
Eco130I CCWWGG 2 cut(s) 1698, 2131
Eco147I AGGCCT 1 cut(s) 919
Eco24I GRGCYC 2 cut(s) 1359, 1590
Eco31I GGTCTC 1 cut(s) 1774
Eco47I GGWCC 5 cut(s) 102, 521, 752, 1076, 1133
Eco53kI GAGCTC 2 cut(s) 1357, 1588
Eco57I CTGAAG 6 cut(s) 1605, 1655, 2123, 2691, 2727, 2799
Eco88I CYCGRG 1 cut(s) 2253
EcoICRI GAGCTC 2 cut(s) 1357, 1588
EcoNI CCTNNNNNAGG 1 cut(s) 1104
EcoO109I RGGNCCY 4 cut(s) 81, 521, 1555, 1673
EcoRI GAATTC 1 cut(s) 2536
EcoRII CCWGG 4 cut(s) 517, 1105, 1129, 2540
EcoT14I CCWWGG 2 cut(s) 1698, 2131
EcoT38I GRGCYC 2 cut(s) 1359, 1590
ErhI CCWWGG 2 cut(s) 1698, 2131
FaeI CATG 8 cut(s) 143, 377, 1355, 1702, 1750, 2020, 2059, 2420
FaqI GGGAC 2 cut(s) 223, 2553
FatI CATG 8 cut(s) 139, 373, 1351, 1698, 1746, 2016, 2055, 2416
FauI CCCGC 3 cut(s) 200, 1173, 2036
FauNDI CATATG 1 cut(s) 162
FbaI TGATCA 3 cut(s) 411, 783, 1333
FriOI GRGCYC 2 cut(s) 1359, 1590
FspBI CTAG 8 cut(s) 381, 453, 512, 540, 1670, 2318, 2363, 2640
GlaI GCGC 1 cut(s) 1433
GsuI CTGGAG 4 cut(s) 501, 1089, 2050, 2351
HaeIII GGCC 6 cut(s) 83, 467, 710, 919, 1556, 1674
HapII CCGG 2 cut(s) 707, 1876
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 1434
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 143, 377, 1355, 1702, 1750, 2020, 2059, 2420
Hin6I GCGC 1 cut(s) 1432
HinP1I GCGC 1 cut(s) 1432
HindIII AAGCTT 1 cut(s) 1637
HinfI GANTC 9 cut(s) 55, 590, 1043, 1277, 1397, 1784, 2042, 2386, 2698
HpaII CCGG 2 cut(s) 707, 1876
HphI GGTGA 7 cut(s) 16, 137, 612, 618, 1437, 1667, 2114
Hpy166II GTNNAC 8 cut(s) 656, 695, 725, 755, 902, 1907, 2266, 2315
Hpy8I GTNNAC 8 cut(s) 656, 695, 725, 755, 902, 1907, 2266, 2315
Hpy99I CGWCG 1 cut(s) 2907
HpyCH4III ACNGT 6 cut(s) 271, 1250, 1889, 1898, 2221, 2871
HpyCH4IV ACGT 3 cut(s) 113, 559, 2464
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 497, 707, 737, 809, 2033, 2813
HpyF3I CTNAG 8 cut(s) 85, 1050, 1551, 1640, 1820, 2051, 2149, 2499
HpySE526I ACGT 3 cut(s) 113, 559, 2464
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 143, 377, 1355, 1702, 1750, 2020, 2059, 2420
HspAI GCGC 1 cut(s) 1432
KpnI GGTACC 3 cut(s) 184, 1893, 2630
Ksp22I TGATCA 3 cut(s) 411, 783, 1333
Kzo9I GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
LguI GCTCTTC 4 cut(s) 25, 1748, 1820, 2390
LweI GCATC 4 cut(s) 9, 289, 1255, 2188
MaeI CTAG 8 cut(s) 381, 453, 512, 540, 1670, 2318, 2363, 2640
MaeII ACGT 3 cut(s) 113, 559, 2464
MaeIII GTNAC 3 cut(s) 260, 1531, 2126
MalI GATC 8 cut(s) 228, 413, 785, 845, 1335, 1369, 1659, 2000
MbiI CCGCTC 1 cut(s) 1684
MboI GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
MfeI CAATTG 1 cut(s) 2922
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
MlsI TGGCCA 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 4 cut(s) 599, 1406, 2380, 2692
MmeI TCCRAC 2 cut(s) 2085, 2834
Mox20I TGGCCA 1 cut(s) 467
MroXI GAANNNNTTC 2 cut(s) 1042, 2442
MscI TGGCCA 1 cut(s) 467
MseI TTAA 9 cut(s) 200, 350, 434, 888, 1295, 1320, 1722, 2156, 2210
Msp20I TGGCCA 1 cut(s) 467
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 2 cut(s) 707, 1876
MspR9I CCNGG 4 cut(s) 519, 1107, 1131, 2542
MunI CAATTG 1 cut(s) 2922
Mva1269I GAATGC 2 cut(s) 2442, 2514
MvaI CCWGG 4 cut(s) 519, 1107, 1131, 2542
MwoI GCNNNNNNNGC 7 cut(s) 41, 497, 707, 737, 809, 2033, 2813
NcoI CCATGG 1 cut(s) 1698
NdeI CATATG 1 cut(s) 162
NdeII GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
NlaIII CATG 8 cut(s) 143, 377, 1355, 1702, 1750, 2020, 2059, 2420
NmuCI GTSAC 2 cut(s) 260, 1531
NspI RCATGY 1 cut(s) 2059
PaeR7I CTCGAG 1 cut(s) 2253
PceI AGGCCT 1 cut(s) 919
PciSI GCTCTTC 4 cut(s) 25, 1748, 1820, 2390
PctI GAATGC 2 cut(s) 2442, 2514
PdmI GAANNNNTTC 2 cut(s) 1042, 2442
PfeI GAWTC 5 cut(s) 55, 1043, 1277, 1784, 2042
PflMI CCANNNNNTGG 1 cut(s) 1205
PfoI TCCNGGA 3 cut(s) 1105, 1129, 2540
PleI GAGTC 4 cut(s) 598, 1405, 2380, 2692
PpsI GAGTC 4 cut(s) 598, 1405, 2380, 2692
Ppu21I YACGTR 1 cut(s) 2465
PpuMI RGGWCCY 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 200
Psp124BI GAGCTC 2 cut(s) 1359, 1590
Psp1406I AACGTT 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 4 cut(s) 517, 1105, 1129, 2540
PspGI CCWGG 4 cut(s) 517, 1105, 1129, 2540
PspPI GGNCC 8 cut(s) 81, 102, 521, 752, 1076, 1133, 1555, 1673
PspPPI RGGWCCY 1 cut(s) 521
PsrI GAACNNNNNNTAC 2 cut(s) 2922, 2954
PstI CTGCAG 3 cut(s) 814, 1681, 2818
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 1 cut(s) 226
SacI GAGCTC 2 cut(s) 1359, 1590
SapI GCTCTTC 4 cut(s) 25, 1748, 1820, 2390
SaqAI TTAA 9 cut(s) 200, 350, 434, 888, 1295, 1320, 1722, 2156, 2210
Sau3AI GATC 8 cut(s) 226, 411, 783, 843, 1333, 1367, 1657, 1998
Sau96I GGNCC 8 cut(s) 81, 102, 521, 752, 1076, 1133, 1555, 1673
SchI GAGTC 4 cut(s) 599, 1406, 2380, 2692
ScrFI CCNGG 4 cut(s) 519, 1107, 1131, 2542
SduI GDGCHC 7 cut(s) 553, 1359, 1590, 1909, 2121, 2317, 2402
SfaNI GCATC 4 cut(s) 9, 289, 1255, 2188
SfcI CTRYAG 4 cut(s) 582, 810, 1677, 2814
Sfr274I CTCGAG 1 cut(s) 2253
SinI GGWCC 5 cut(s) 102, 521, 752, 1076, 1133
SlaI CTCGAG 1 cut(s) 2253
SmlI CTYRAG 2 cut(s) 349, 2253
SmoI CTYRAG 2 cut(s) 349, 2253
SseBI AGGCCT 1 cut(s) 919
SspI AATATT 1 cut(s) 2230
SspMI CTAG 8 cut(s) 381, 453, 512, 540, 1670, 2318, 2363, 2640
SstI GAGCTC 2 cut(s) 1359, 1590
StuI AGGCCT 1 cut(s) 919
StyD4I CCNGG 4 cut(s) 517, 1105, 1129, 2540
StyI CCWWGG 2 cut(s) 1698, 2131
TaaI ACNGT 6 cut(s) 271, 1250, 1889, 1898, 2221, 2871
TaiI ACGT 3 cut(s) 116, 562, 2467
TaqI TCGA 3 cut(s) 547, 1340, 2254
TatI WGTACW 2 cut(s) 835, 2075
TauI GCSGC 2 cut(s) 399, 500
TfiI GAWTC 5 cut(s) 55, 1043, 1277, 1784, 2042
Tru1I TTAA 9 cut(s) 200, 350, 434, 888, 1295, 1320, 1722, 2156, 2210
Tru9I TTAA 9 cut(s) 200, 350, 434, 888, 1295, 1320, 1722, 2156, 2210
TscAI CASTG 7 cut(s) 265, 475, 1255, 1743, 2674, 2710, 2782
TseFI GTSAC 2 cut(s) 260, 1531
Tsp45I GTSAC 2 cut(s) 260, 1531
TspGWI ACGGA 1 cut(s) 1994
TspRI CASTG 7 cut(s) 265, 475, 1255, 1743, 2674, 2710, 2782
Van91I CCANNNNNTGG 1 cut(s) 1205
Vha464I CTTAAG 1 cut(s) 349
VneI GTGCAC 2 cut(s) 1905, 2313
VpaK11BI GGWCC 5 cut(s) 102, 521, 752, 1076, 1133
VspI ATTAAT 1 cut(s) 200
XagI CCTNNNNNAGG 1 cut(s) 1104
XapI RAATTY 3 cut(s) 1218, 2536, 2877
XbaI TCTAGA 1 cut(s) 2362
XceI RCATGY 1 cut(s) 2059
XhoI CTCGAG 1 cut(s) 2253
XmnI GAANNNNTTC 2 cut(s) 1042, 2442
XspI CTAG 8 cut(s) 381, 453, 512, 540, 1670, 2318, 2363, 2640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.