Rorug02G0483100

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011587
Physical Location & Seq
Reverse (-)
61296909 .. 61304818
7910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0483100.1

Sequence Viewer

Length: 3702 bp
ATGGTAAGGACGAAGAAAGGAGGACGACCGCCGACTCTGATTCCAGACGAGGCAAGGATCCGAATACGTGACATACTCCAGCAGTTCCTGGAGTCCAATGAAGAAGTTTACACATTTGGGACAGACCTGACGGTTAATGAACGGGCTTACGTCCACGAACAGTGCAGGAAGATGGATATGAAATCCAAGAGCTCCGGGTCGGGTAAAACGCGCAAGGTTTCGGTTTACAGGCTTTTTAGGATGGAGGCAACCAGCAATGGTAAGGAAAGAATCCCTCAGGTGATGTTTTCCGGAGACACAAGAGAAGTTTTGGGGGATTTGTTTACGTGGTACCCACCCGGAGATGGACAGGAAGCCAAAGAAAGGATCCGTGAAGATAGTGACAAGTTTCAGAAACTCCACCGCAAGAAAGACCCGCTGTTCTGCAGACCTTCAATGAGCCAGAACGAAATTGCAAAGAAAGTGGAAGTGCAGGCTGCTAAAATGAGAAAAGCCAAGTTTACTAAAGAGAGGTCCAAGCTACCCATGGCATCCTACAGGGATGTGGTAACGTCAGCAATTGAATCTCACCAGGTTGTTCTTGTATCTGGTGAGACTGGGTGCGGAAAGACCACACAGGTGCCACAATATCTTTTGGACCATATGTGGGGTAAGGGTGAGGCATGTAAAATAGTGTGCACTCAACCCAGGCGTATCTCAGCCATATCAGTTGCTGAGAGAATCTCTTATGAAAGAGGGCAGAATGTTGGGGAGGATATTGGATACAAGATACGTTTGGAAAGCAAAGGTGGGAGGCACTCATCTATTGTGTTCTGCACAAATGGGATTCTACTGAGGATTCTAATTTCCAGAGGCGGTGGTAGGTCAAAAACAGAAGCATCGAATAAAGAAACCAAACAAGATCTCTCTGACATAACTCACATTATTGTGGATGAAATTCATGAAAGGGATCGCCACTCTGATTTCATTCTGGCTATTCTCAGAGACATGCTTCCTTCACATCCTCATCTGCGTCTGATATTGATGAGTGCGACTGTTGATGTGGAACGGTTTTCACAATACTTTGGTGGCTGCCCAATTATCCGTGTCCCAGGGTTCACTTATCCTGTCAAATCTTTCTATTTGGAGGATGTATTGTCTATCTTGAGATCTGTAGAAAATAATCACTTAGATAACCCTGCATTAGGTGCCTCAACTGCAGACCGTGACTTATCAGAGGAAGATAAGATTGCTTTAGATGAAGCAATTAGTTTGGCTTGGTCAGATGATGAATTTGATCCACTTCTAGAATTGGTTTCTACTGAAGGAACCCGTAAAATCTTTAATTACCAGCATTCTGTAACTGGACTTACACCATTAATGGTATTTGCTGGAAAGGGTAGAGTAGGAGATGTTTGCATGCTGCTATCATTTGGTGCAGACTGCCAATTACGGGCCAAGGATGGAACCACTGCATTAGAGTTGGCTGAGCGGGTAAACCAATTAGAATCTGCTCAGATGTTGAAAGAGCATATGGAAAGTGCTCTTTCTAACTCTGTGGAAGAACAAAATTTGCTCAATAAATATCTCCAATATTTTAATCCTGAGAATATTGATTTCGTTCTTATCGAGCAATTGTTAGGGAAAATTTTTAGTGATTCGAAAGATGGAGCTATTCTTGTTTTTCTTCCAGGGTGGGATGATATAAATAAAATGCGGGACAGATTACTCACGAACCCATATTTCAGAGATCCTTCTAAGTGTGTGATTATATCTCTACATTCAATGGTTCCAGCTGCTGAACAGAAGAAGGTCTTCATTCGTCCACCTAGTGGTTGCCGAAAAATTGTTCTTTCAACCAATATTGCTGAAACTGCAGTCACTATTGATGATGTTGTTTATGTTATAAACACGGGTCGGATGAAAGAAAAAAATTATGATCCTTTTAGTAATGTTTCAACTCTTCAATCTTCTTGGGTGTCAAAAGCGAATGCCAAGCAGCGACAGGGGCGTGCGGGCCGTTGTCAGCCTGGAATCTGTTATCATCTTTACTCTAAACTTCGAGCAGCTGCATTGTCAGATTTTCAGGTGCCTGAGATTAGGAGAATGCCAATCGAGGAACTTTGCCTTCAGGTGAAGTTGCTTGATCCAGATTGCAAGATAGACGAGTTCCTAAATAAGACGCTGGATCCTCCTCATTCTGAGACCATACACAATGCGATTTCTGTTCTTCGAGATATTGGGGCTTTGTCGGTGGACGATAAACTTACAGAACTTGGGGAAAAGCTAGGTTCTTTACCTGTTCATCCATTGACAAGCAAGATGCTATTCTTTGCCACACTGATGAACTGTCTTGACCCAGCTTTAACTCTGGCCTGTGCTGCCGACTACAAGGATCCATTCTCTCTTCCTATGATGCCTGAGGAAAAGGAGAAAGCTGCTGCTGCTAAGGCTGAACTATGTTCATTGTATGGTGGGAACAGTGATCAGCTAGCAGTCATAGCAGCATTCGACTGCTGGAAGATTGCAAAAGAACAGGGTAAAGAGAATCGGTTCTGTGCTAAATACTTTGTCTCTGCAAGTACCATGCAGATGCTATCTGGTATGCGAAAGCAACTCCAAACAGAGCTAATTCGGCTGGGGTTTATTCCTGAGGATGTGTCTAGATGTAGCTTGAATGCACGCAATCCAGGTATAATCCACGCTGTCCTTGTGGCTGGTCTGTATCCCATGTTGGGAAGATTGCTTTCTTCTACTAAGAGTGGAAAAAAAAAAGGATCCTTTATAGAAATTCCTGATGGTAGTAACGTTCGTTTGCACCCTCGATCTGCTAATTTTAAAATAGCGTTCAAGGCACGTGGTGGCTCTCCTTTGGTTGTATATGATGAGATAACCCGTGGGGATGGGGGTTCGAATATACGAAACTGTACTGTTGTCGGGCCACTTCCATTATTATTGCTGTCGACAGAAATAGCTGTTGCTCCTGCCAATGATGGTGAGGGAAGTGATGTCGATGATAATGAGTATGAAAGTGATGAAGATGCAATGAAGATAGATTCTAAGTCGGGTGGACAGGCCGAAGAGAAGATTATGTCATCACCTGATAAATCCGTCATGGTTATAGTGGATGGGTGGCTTTGTTTTGAGTCGAAAGCTCTTGACGTTGCTCAGATCTATTGCTTGAGGGAACGCCTGTCTGCAGCAATACTATTTAAGGTTACTCATCCAAAGGAAGTTTTACCTCCTGATCTTGGGGCATCTGTTTATGCTATAGCCTGCGTTCTATCTTATGATGGTCTATCTGGGATTCCATTGCCAAAGGCTATGGATTCGCCGAATTCAGATTTAGAAGAACTTGAGATCGGTAAAGATGGAAAGGCACAGTTTAAAGTGACAGGAAATAAAGCAGCAAAATCAAAGGAGCCATTACGTCAGAGAGTTTGTCAGAAGTCTCTATCGCGAGCTACTGATTTACCTGCCTTAAATGGAGTTGCAAAGTTGAGTGACGACCCATCAATTCAGAGTGTCCCCCCACCAGCTTTCGCGAACGCTACTGATTTACCTGCCTTAATAAATGGAGTTGCAAAGTTGAGTGGCGACCGATCAATTCACAGTGTTCCCCCACCAGCTTTTGCGAACACAGTCCCGCCGCCCACTTCACAAGTTCCAGCAGGTCCTGTAGCAGGAAACAAGCGGAAGAAGCGCCGGAATCAGGCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04320 map04320
Pfam Domains
Protein Families
FH2

Protein Analysis

1233

Amino Acids

136.25

Weight (kDa)

7.98

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 20 - 77 2.3e-08 R3H domain
DEAD PF00270 186 - 347 6.3e-12 DEAD/DEAH box helicase
Helicase_C PF00271 539 - 668 6.9e-11 Helicase conserved C-terminal domain
HA2_C PF21010 763 - 824 1.6e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 893 - 983 5.7e-11 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1886
Acc36I ACCTGC 3 cut(s) 3463, 3550, 3641
Acc65I GGTACC 1 cut(s) 330
AccB1I GGYRCC 4 cut(s) 330, 619, 1187, 2068
AccB7I CCANNNNNTGG 1 cut(s) 1811
AccBSI CCGCTC 1 cut(s) 1471
AccI GTMKAC 1 cut(s) 2942
AccII CGCG 3 cut(s) 211, 3439, 3524
AccIII TCCGGA 1 cut(s) 290
AclI AACGTT 1 cut(s) 2787
AcsI RAATTY 6 cut(s) 936, 1271, 1549, 1626, 2769, 3316
AcuI CTGAAG 2 cut(s) 1323, 2093
AcvI CACGTG 1 cut(s) 2837
AdeI CACNNNGTG 2 cut(s) 1811, 2840
AfaI GTAC 3 cut(s) 332, 2563, 2908
AgsI TTSAA 9 cut(s) 435, 563, 1504, 1764, 1836, 1938, 1946, 2656, 2830
AjnI CCWGG 7 cut(s) 87, 570, 686, 1090, 1669, 2008, 2668
AleI CACNNNNGTG 1 cut(s) 617
Alw21I GWGCWC 3 cut(s) 194, 680, 1525
Alw26I GTCTC 6 cut(s) 288, 587, 978, 2177, 2557, 3435
Alw44I GTGCAC 1 cut(s) 676
AlwNI CAGNNNCTG 4 cut(s) 88, 713, 1778, 3656
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 5 cut(s) 1434, 1996, 2352, 2918, 3054
ApaLI GTGCAC 1 cut(s) 676
ApoI RAATTY 6 cut(s) 936, 1271, 1549, 1626, 2769, 3316
AseI ATTAAT 1 cut(s) 1358
Asp700I GAANNNNTTC 2 cut(s) 1793, 2531
Asp718I GGTACC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 213, 3684
AspS9I GGNCC 6 cut(s) 513, 637, 1434, 1996, 2918, 3653
AsuC2I CCSGG 2 cut(s) 196, 339
AsuHPI GGTGA 7 cut(s) 292, 560, 602, 668, 2125, 2987, 3069
AsuII TTCGAA 2 cut(s) 1640, 2891
AsuNHI GCTAGC 1 cut(s) 2470
AvaII GGWCC 3 cut(s) 513, 637, 3653
AxyI CCTNAGG 3 cut(s) 276, 2400, 2631
BaeGI GKGCMC 1 cut(s) 680
BamHI GGATCC 5 cut(s) 57, 366, 2167, 2374, 2756
BanI GGYRCC 4 cut(s) 330, 619, 1187, 2068
BanII GRGCYC 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 2837
BbsI GAAGAC 1 cut(s) 1786
Bbv12I GWGCWC 3 cut(s) 194, 680, 1525
BceAI ACGGC 1 cut(s) 1983
BciT130I CCWGG 7 cut(s) 89, 572, 688, 1092, 1671, 2010, 2670
BciVI GTATCC 2 cut(s) 755, 2715
BclI TGATCA 1 cut(s) 2464
BcnI CCSGG 2 cut(s) 196, 339
BcoDI GTCTC 6 cut(s) 288, 587, 978, 2177, 2557, 3435
BfaI CTAG 5 cut(s) 1286, 1809, 2267, 2471, 2643
BfmI CTRYAG 8 cut(s) 424, 535, 1152, 1197, 1854, 3177, 3249, 3657
BfoI RGCGCY 1 cut(s) 3685
BfuAI ACCTGC 3 cut(s) 3463, 3550, 3641
BfuI GTATCC 2 cut(s) 755, 2715
BglII AGATCT 3 cut(s) 901, 1148, 3150
BlpI GCTNAGC 1 cut(s) 1467
Bme1390I CCNGG 9 cut(s) 89, 196, 339, 572, 688, 1092, 1671, 2010, 2670
Bme18I GGWCC 3 cut(s) 513, 637, 3653
BmgT120I GGNCC 6 cut(s) 513, 637, 1434, 1996, 2918, 3653
BmrFI CCNGG 9 cut(s) 89, 196, 339, 572, 688, 1092, 1671, 2010, 2670
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 7 cut(s) 539, 887, 2292, 2385, 2562, 3010, 3245
BmtI GCTAGC 1 cut(s) 2474
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 1786
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 2 cut(s) 62, 110
Bpu10I CCTNAGC 1 cut(s) 2427
Bpu1102I GCTNAGC 1 cut(s) 1467
Bpu14I TTCGAA 2 cut(s) 1640, 2891
BpuEI CTTGAG 3 cut(s) 1165, 3181, 3356
BpuMI CCSGG 2 cut(s) 196, 339
BsaAI YACGTR 3 cut(s) 68, 327, 2837
BsaI GGTCTC 1 cut(s) 2177
BsaJI CCNNGG 7 cut(s) 525, 686, 1090, 1091, 1437, 1670, 2875
BsaWI WCCGGW 1 cut(s) 290
Bse1I ACTGG 2 cut(s) 601, 1348
Bse21I CCTNAGG 3 cut(s) 276, 2400, 2631
Bse3DI GCAATG 3 cut(s) 262, 3030, 3290
BseAI TCCGGA 1 cut(s) 290
BseBI CCWGG 7 cut(s) 89, 572, 688, 1092, 1671, 2010, 2670
BseDI CCNNGG 7 cut(s) 525, 686, 1090, 1091, 1437, 1670, 2875
BseMI GCAATG 3 cut(s) 262, 3030, 3290
BseNI ACTGG 2 cut(s) 601, 1348
BseRI GAGGAG 1 cut(s) 2163
BseSI GKGCMC 1 cut(s) 680
BseYI CCCAGC 2 cut(s) 2338, 2617
BsgI GTGCAG 4 cut(s) 184, 491, 799, 1437
Bsh1236I CGCG 3 cut(s) 211, 3439, 3524
Bsh1285I CGRYCG 2 cut(s) 29, 3580
BshFI GGCC 5 cut(s) 1436, 1998, 2354, 2920, 3056
BshNI GGYRCC 4 cut(s) 330, 619, 1187, 2068
BsiEI CGRYCG 2 cut(s) 29, 3580
BsiHKAI GWGCWC 3 cut(s) 194, 680, 1525
BsiSI CCGG 4 cut(s) 195, 291, 339, 3685
BslFI GGGAC 5 cut(s) 133, 1073, 1712, 3491, 3608
BsmAI GTCTC 6 cut(s) 288, 587, 978, 2177, 2557, 3435
BsmFI GGGAC 5 cut(s) 133, 1073, 1712, 3491, 3608
BsmI GAATGC 5 cut(s) 1333, 1975, 2091, 2486, 2662
BsnI GGCC 5 cut(s) 1436, 1998, 2354, 2920, 3056
Bso31I GGTCTC 1 cut(s) 2177
Bsp119I TTCGAA 2 cut(s) 1640, 2891
Bsp1286I GDGCHC 3 cut(s) 194, 680, 1525
Bsp13I TCCGGA 1 cut(s) 290
Bsp1720I GCTNAGC 1 cut(s) 1467
Bsp19I CCATGG 1 cut(s) 525
Bsp68I TCGCGA 2 cut(s) 3439, 3524
BspANI GGCC 5 cut(s) 1436, 1998, 2354, 2920, 3056
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 3 cut(s) 211, 3439, 3524
BspHI TCATGA 1 cut(s) 940
BspMAI CTGCAG 4 cut(s) 428, 1201, 1858, 3181
BspMI ACCTGC 3 cut(s) 3463, 3550, 3641
BspOI GCTAGC 1 cut(s) 2474
BspT104I TTCGAA 2 cut(s) 1640, 2891
BspT107I GGYRCC 4 cut(s) 330, 619, 1187, 2068
BspTNI GGTCTC 1 cut(s) 2177
BsrBI CCGCTC 1 cut(s) 1471
BsrDI GCAATG 3 cut(s) 262, 3030, 3290
BsrI ACTGG 2 cut(s) 601, 1348
BssECI CCNNGG 7 cut(s) 525, 686, 1090, 1091, 1437, 1670, 2875
BssT1I CCWWGG 2 cut(s) 525, 1437
Bst2UI CCWGG 7 cut(s) 89, 572, 688, 1092, 1671, 2010, 2670
Bst6I CTCTTC 3 cut(s) 1947, 2391, 3054
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstBAI YACGTR 3 cut(s) 68, 327, 2837
BstBI TTCGAA 2 cut(s) 1640, 2891
BstC8I GCNNGC 8 cut(s) 474, 1400, 1992, 1996, 2472, 2662, 3256, 3441
BstDSI CCRYGG 2 cut(s) 525, 2875
BstENI CCTNNNNNAGG 2 cut(s) 1182, 3660
BstFNI CGCG 3 cut(s) 211, 3439, 3524
BstH2I RGCGCY 1 cut(s) 3685
BstHHI GCGC 2 cut(s) 213, 3684
BstMAI GTCTC 6 cut(s) 288, 587, 978, 2177, 2557, 3435
BstMCI CGRYCG 2 cut(s) 29, 3580
BstNI CCWGG 7 cut(s) 89, 572, 688, 1092, 1671, 2010, 2670
BstNSI RCATGY 3 cut(s) 666, 991, 1402
BstSCI CCNGG 9 cut(s) 87, 194, 337, 570, 686, 1090, 1669, 2008, 2668
BstSFI CTRYAG 8 cut(s) 424, 535, 1152, 1197, 1854, 3177, 3249, 3657
BstSLI GKGCMC 1 cut(s) 680
BstUI CGCG 3 cut(s) 211, 3439, 3524
BstV2I GAAGAC 1 cut(s) 1786
BstX2I RGATCY 9 cut(s) 57, 366, 901, 1148, 1729, 2167, 2374, 2756, 3150
BstYI RGATCY 9 cut(s) 57, 366, 901, 1148, 1729, 2167, 2374, 2756, 3150
Bsu36I CCTNAGG 3 cut(s) 276, 2400, 2631
BsuI GTATCC 2 cut(s) 755, 2715
BsuRI GGCC 5 cut(s) 1436, 1998, 2354, 2920, 3056
BtgI CCRYGG 2 cut(s) 525, 2875
BtsI GCAGTG 1 cut(s) 1449
BtsIMutI CAGTG 5 cut(s) 167, 1449, 2318, 2467, 3598
BtuMI TCGCGA 2 cut(s) 3439, 3524
BveI ACCTGC 3 cut(s) 3463, 3550, 3641
Cac8I GCNNGC 8 cut(s) 474, 1400, 1992, 1996, 2472, 2662, 3256, 3441
CaiI CAGNNNCTG 4 cut(s) 88, 713, 1778, 3656
CciI TCATGA 1 cut(s) 940
CfoI GCGC 2 cut(s) 213, 3684
Cfr13I GGNCC 6 cut(s) 513, 637, 1434, 1996, 2918, 3653
CseI GACGC 2 cut(s) 1001, 2170
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 3 cut(s) 331, 2562, 2907
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 2818, 2853
CviAII CATG 8 cut(s) 526, 663, 941, 988, 1399, 2566, 2710, 3094
CviQI GTAC 3 cut(s) 331, 2562, 2907
DraI TTTAAA 2 cut(s) 2818, 3367
DraIII CACNNNGTG 2 cut(s) 1811, 2840
Eam1104I CTCTTC 3 cut(s) 1947, 2391, 3054
EarI CTCTTC 3 cut(s) 1947, 2391, 3054
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 525, 1437
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 2177
Eco47I GGWCC 3 cut(s) 513, 637, 3653
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 1323, 2093
Eco72I CACGTG 1 cut(s) 2837
Eco81I CCTNAGG 3 cut(s) 276, 2400, 2631
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 2 cut(s) 1182, 3660
EcoO109I RGGNCCY 1 cut(s) 3653
EcoRI GAATTC 1 cut(s) 3316
EcoRII CCWGG 7 cut(s) 87, 570, 686, 1090, 1669, 2008, 2668
EcoT14I CCWWGG 2 cut(s) 525, 1437
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 525, 1437
FaeI CATG 8 cut(s) 529, 666, 944, 991, 1402, 2569, 2713, 3097
FalI AAGNNNNNCTT 2 cut(s) 1778, 1810
FaqI GGGAC 5 cut(s) 133, 1073, 1712, 3491, 3608
FatI CATG 8 cut(s) 525, 662, 940, 987, 1398, 2565, 2709, 3093
FauI CCCGC 5 cut(s) 423, 1464, 1689, 1987, 3633
FauNDI CATATG 2 cut(s) 642, 1512
FbaI TGATCA 1 cut(s) 2464
FblI GTMKAC 1 cut(s) 2942
FriOI GRGCYC 1 cut(s) 194
FspBI CTAG 5 cut(s) 1286, 1809, 2267, 2471, 2643
GlaI GCGC 2 cut(s) 212, 3683
GsaI CCCAGC 2 cut(s) 2342, 2621
GsuI CTGGAG 2 cut(s) 62, 110
HaeII RGCGCY 1 cut(s) 3685
HaeIII GGCC 5 cut(s) 1436, 1998, 2354, 2920, 3056
HapII CCGG 4 cut(s) 195, 291, 339, 3685
HgaI GACGC 2 cut(s) 1001, 2170
HhaI GCGC 2 cut(s) 213, 3684
Hin1II CATG 8 cut(s) 529, 666, 944, 991, 1402, 2569, 2713, 3097
Hin6I GCGC 2 cut(s) 211, 3682
HinP1I GCGC 2 cut(s) 211, 3682
HincII GTYRAC 1 cut(s) 2943
HindII GTYRAC 1 cut(s) 2943
HpaII CCGG 4 cut(s) 195, 291, 339, 3685
HphI GGTGA 7 cut(s) 292, 560, 602, 668, 2125, 2987, 3069
HpyAV CCTTC 6 cut(s) 441, 1005, 1298, 1743, 1783, 2117
HpyCH4IV ACGT 9 cut(s) 67, 150, 326, 551, 772, 2787, 2836, 3141, 3409
HpySE526I ACGT 9 cut(s) 67, 150, 326, 551, 772, 2787, 2836, 3141, 3409
Hsp92II CATG 8 cut(s) 529, 666, 944, 991, 1402, 2569, 2713, 3097
HspAI GCGC 2 cut(s) 211, 3682
Kpn2I TCCGGA 1 cut(s) 290
KpnI GGTACC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 2464
LmnI GCTCC 4 cut(s) 197, 1649, 2965, 3400
LweI GCATC 7 cut(s) 539, 887, 2292, 2385, 2562, 3010, 3245
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 5 cut(s) 1286, 1809, 2267, 2471, 2643
MaeII ACGT 9 cut(s) 67, 150, 326, 551, 772, 2787, 2836, 3141, 3409
MbiI CCGCTC 1 cut(s) 1471
MfeI CAATTG 2 cut(s) 558, 1613
MflI RGATCY 9 cut(s) 57, 366, 901, 1148, 1729, 2167, 2374, 2756, 3150
MhlI GDGCHC 3 cut(s) 194, 680, 1525
MlyI GAGTC 3 cut(s) 28, 101, 3134
MmeI TCCRAC 1 cut(s) 1877
MroI TCCGGA 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 1793, 2531
MslI CAYNNNNRTG 4 cut(s) 617, 926, 2321, 2570
MspA1I CMGCKG 3 cut(s) 418, 1775, 2048
MspI CCGG 4 cut(s) 195, 291, 339, 3685
MspR9I CCNGG 9 cut(s) 89, 196, 339, 572, 688, 1092, 1671, 2010, 2670
MunI CAATTG 2 cut(s) 558, 1613
Mva1269I GAATGC 5 cut(s) 1333, 1975, 2091, 2486, 2662
MvaI CCWGG 7 cut(s) 89, 572, 688, 1092, 1671, 2010, 2670
MvnI CGCG 3 cut(s) 211, 3439, 3524
NciI CCSGG 2 cut(s) 196, 339
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 2 cut(s) 642, 1512
NheI GCTAGC 1 cut(s) 2470
NlaIII CATG 8 cut(s) 529, 666, 944, 991, 1402, 2569, 2713, 3097
NmuCI GTSAC 6 cut(s) 68, 380, 1205, 1858, 3370, 3482
NruI TCGCGA 2 cut(s) 3439, 3524
NspI RCATGY 3 cut(s) 666, 991, 1402
NspV TTCGAA 2 cut(s) 1640, 2891
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 1402
PagI TCATGA 1 cut(s) 940
PasI CCCWGGG 1 cut(s) 1091
PcsI WCGNNNNNNNCGW 1 cut(s) 1605
PctI GAATGC 5 cut(s) 1333, 1975, 2091, 2486, 2662
PdmI GAANNNNTTC 2 cut(s) 1793, 2531
PflMI CCANNNNNTGG 1 cut(s) 1811
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 3 cut(s) 28, 100, 3133
PmaCI CACGTG 1 cut(s) 2837
PmlI CACGTG 1 cut(s) 2837
PpsI GAGTC 3 cut(s) 28, 100, 3133
Ppu21I YACGTR 3 cut(s) 68, 327, 2837
PpuMI RGGWCCY 1 cut(s) 3653
PshBI ATTAAT 1 cut(s) 1358
PsiI TTATAA 1 cut(s) 1886
Psp124BI GAGCTC 1 cut(s) 194
Psp1406I AACGTT 1 cut(s) 2787
Psp5II RGGWCCY 1 cut(s) 3653
Psp6I CCWGG 7 cut(s) 87, 570, 686, 1090, 1669, 2008, 2668
PspCI CACGTG 1 cut(s) 2837
PspFI CCCAGC 2 cut(s) 2338, 2617
PspGI CCWGG 7 cut(s) 87, 570, 686, 1090, 1669, 2008, 2668
PspPI GGNCC 6 cut(s) 513, 637, 1434, 1996, 2918, 3653
PspPPI RGGWCCY 1 cut(s) 3653
PstI CTGCAG 4 cut(s) 428, 1201, 1858, 3181
PstNI CAGNNNCTG 4 cut(s) 88, 713, 1778, 3656
PsuI RGATCY 9 cut(s) 57, 366, 901, 1148, 1729, 2167, 2374, 2756, 3150
PvuII CAGCTG 2 cut(s) 1775, 2048
RruI TCGCGA 2 cut(s) 3439, 3524
RsaI GTAC 3 cut(s) 332, 2563, 2908
RsaNI GTAC 3 cut(s) 331, 2562, 2907
RseI CAYNNNNRTG 4 cut(s) 617, 926, 2321, 2570
SacI GAGCTC 1 cut(s) 194
SalI GTCGAC 1 cut(s) 2941
Sau96I GGNCC 6 cut(s) 513, 637, 1434, 1996, 2918, 3653
SchI GAGTC 3 cut(s) 28, 101, 3134
ScrFI CCNGG 9 cut(s) 89, 196, 339, 572, 688, 1092, 1671, 2010, 2670
SduI GDGCHC 3 cut(s) 194, 680, 1525
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 7 cut(s) 539, 887, 2292, 2385, 2562, 3010, 3245
SfcI CTRYAG 8 cut(s) 424, 535, 1152, 1197, 1854, 3177, 3249, 3657
SfuI TTCGAA 2 cut(s) 1640, 2891
SinI GGWCC 3 cut(s) 513, 637, 3653
SmiMI CAYNNNNRTG 4 cut(s) 617, 926, 2321, 2570
SmlI CTYRAG 3 cut(s) 1144, 3160, 3335
SmoI CTYRAG 3 cut(s) 1144, 3160, 3335
SphI GCATGC 1 cut(s) 1402
SspI AATATT 3 cut(s) 1574, 1591, 1843
SspMI CTAG 5 cut(s) 1286, 1809, 2267, 2471, 2643
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 9 cut(s) 87, 194, 337, 570, 686, 1090, 1669, 2008, 2668
StyI CCWWGG 2 cut(s) 525, 1437
TaiI ACGT 9 cut(s) 70, 153, 329, 554, 775, 2790, 2839, 3144, 3412
TaqII GACCGA 1 cut(s) 3594
TatI WGTACW 1 cut(s) 2906
TauI GCSGC 1 cut(s) 3631
TscAI CASTG 5 cut(s) 167, 1456, 2325, 2467, 3598
TseFI GTSAC 6 cut(s) 68, 380, 1205, 1858, 3370, 3482
Tsp45I GTSAC 6 cut(s) 68, 380, 1205, 1858, 3370, 3482
TspGWI ACGGA 3 cut(s) 359, 1073, 3079
TspRI CASTG 5 cut(s) 167, 1456, 2325, 2467, 3598
Van91I CCANNNNNTGG 1 cut(s) 1811
VneI GTGCAC 1 cut(s) 676
VpaK11BI GGWCC 3 cut(s) 513, 637, 3653
VspI ATTAAT 1 cut(s) 1358
XagI CCTNNNNNAGG 2 cut(s) 1182, 3660
XapI RAATTY 6 cut(s) 936, 1271, 1549, 1626, 2769, 3316
XbaI TCTAGA 2 cut(s) 1285, 2642
XceI RCATGY 3 cut(s) 666, 991, 1402
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 2942
XmnI GAANNNNTTC 2 cut(s) 1793, 2531
XspI CTAG 5 cut(s) 1286, 1809, 2267, 2471, 2643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.