RchiOBHm_Chr2g0160331

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011587
Physical Location & Seq
Forward (+)
76236806 .. 76241401
4596 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52880

Sequence Viewer

Length: 2979 bp
ATGGTGATTTGGGAAGGGATGCAAATGAGAAGAGCCAGTTATGCGGTTTTCGTGATTCTGCTCTGTGCATTGGTCATTGGGGCCTCAGAGGGCGTCAGGAGGACCAAAGAAACGTTTTATGGAGGTGATTGGGTTTCGCATGAGGAGGAGATGGATGAACATATGGCGGAGCAGGTATGGTACCATTGCAAGAAAGAATTAATAGGCGGGACAGATGGGGTTGAAGATCTTGATTTGTATATTCCACAGGAAGCAGCCAGTGACGAAACAGTGTTCTTTTCAAAGAGCAACATACACGATGCGATTTTAGTTCTTCCTCCTGATGTGAAACAAACACTTGTTGATTGCTTAAGAAAGAAAGGGATTGTGTTTCATGCTCCTAGCAAAAGGAATAGCGGCAACAACTGGTTGATCAACTACTTTGAGTGGCTTTTAAGTTCGGATATTGCTCCTAAAAGATATTTGGCCAGTGGTTCCCCCAAGTCAAAAGCACCAGCCGCTGTTCCATCTCTAGCTCCAGGTCCTGCTTCTAAATTGTCTAGCGGTCGAGCACCTCCACGTTCAGTTCTATCTCCAAGCCCTACAGATGAGTCAGATAACAATGAAGCACCAGCACCTTCACCTTCACCTTCACCTCCTAAACAAAGTGGGAAGGGAAGTCCACCTCCCAAACCAAGTGGGAAAGTAGCTGCTTCAAGTCCACCAGCAAAACCGGCCAAAACAAAAAGTCCACCAGCAAAACCAGCCAAAACAAATGGTCCACCATCAAAGGATGGCAGTATGAGTATGATCAGAATAATTGGTATTGCTAGTGCTGCAGTAGTGATAATTTTGCTTGTTGTACTGCCGATCTGCTGCTGCTGTCTAAAGAAAAGAAGAAGGAGAAGAAAAGTTAATCCGAGAAGTGTACCAAAGGATGACAGGCCTCTTCTCTATATATCTTCTTCTGGTTCTTCACAAATATCCAATAGTTTAGGTTCAAGTACCAGAGAGTTCAACGCTGGCGGAAAGTTGGGAAATTTGCCTATGAAAAATGAAAAAGGAAATGATTCTTCTGAGGCAAAGTCAGAAGGTACAGCAGGACCACCAAACCCTCCTGTACCACCTCCTCCAGGAAAAGCAGCTCCTCCGCCTCCTGGACCACCACCACCGCCTCCTCTTCGTCCTCGCCCGCCACCAGTACCTCCCAAAAATGCACCTAAACCAATGGCTGGTAAGACCCAAATTCCACCTAAAGGAAATCTCAAAGCAAACAGTGGCGATAATGCTGATGCTGGAAGTGAATCTGGAAAGACCAAGTTAAAACCATTCTTCTGGGATAAGGTTAATGCCAACCCTGATCAATCGATGGTCTGGCATGAGCTCAAAGAAGGATCATTCCAGTTCAATGAGGAGAAGATAGAGTCTCTATTTGGTTATACAAAGAGCCAAAATGAGCGCAAAAAGGTTTCGCCATCGGAACCTGCAATCCAGTTTATCCAAATTATTGACAAAAAGAAAGCACAAAATCTATCAATTCTTCTGCGAGCATTGAATGTGACAACGGAAGAAGTTTCTGAGGCCCTCCGAGAAGGCAACGAGCTTCCTGTGGAGCTCCTCCAAACCCTGTTGAAGATGGCACCAACTTCAGAAGAGGAACTGAAGCTTAGGTTATACACAGGTGATCTTGCTCTACTAGGCCCTGCAGAGCGGTTCCTCAAAGCCATGGTTGAGATACCATTTGCTTTTAAGCGGATGGAGGCACTGCTCTTCATGTGTTCCTTCAATGAGGAGGTCTCTAACACTAAAGAATCCTTTGCAACTTTAGAGGTTGCTTGCAGCAAGCTCAGGAAGAGCAGGCTATTCCTAAAACTTTTGGAAGCCGTACTAAAAACTGGCAACCGGATGAATGATGGTACCTACCGTGGTGGTGCACAAGCATTTAGGCTTGATACTCTCTTGAAACTGGCTGATGTAAAAGGAACTGATGGCAAGACCACACTCTTGCACTTTGTCGTTCAAGAGATCATCCGTTATGAGGGCATGAAAGCTGCCCGCAGAGCCAGAGAATCAAACCTGAGCATGTCCAACTCCAGTATGAGTACGGAGAATTTTGTTGAAGAGGTTGGTGAGGAATTAGCAGAGCACTATCGTAACCTTGGTCTTCAGGTTGTCTCAGGTTTAAGCGATGAACTTCAAGATGTGAAAAAGGCAGCAGTTGTAGATGCCGATAGCTTAACTGCAACAGTTTCAAATATTGGTACAAATCTAATAAAAGCTCGAGACTTTGTAAACACCGACTTGAAGAATAATCTGGATGAAGACAGCGAGTTCCATCGTGCACTAGCCAGTTTTCTGGAGCGTGCAGAAGGAGAAACTACATGTCTTCTAGAAGAAGAAAGGAGGATAACGACTCTTGTGAAGAGCACGGCAGATTACTTTCATGGGAACGCAATGAAGGATGAAGGCATTCGTTTGTTTGCCATTGTACGTGATTTTTTGGTAATACTAAATAAGGTATGTGCTGAGATTAGAATGCAAGCAATGAAGCAAGCGAAGACGAATTCCAGGAAAGAGGCTCTCGCACAAGCAAGTCCCAAGAAGGAGGCTCTCACACAAGCAAAATCAGAAAACCAGTCAGAAATGAATAAGGTACCCACAGAAGCTAGTTCAGAAAAGAAAGAGAAGGTTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGACTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACAGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACTGAAGCAAGTTCAGAAAATAAAGAGAAGGCTCCAACTGCAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACAGAAGCAAGTTCAGAAAAACCGTCAGAAATTCAACAGCAACATTCAGATATTCGTCGGCGACTCTTTCCAGCAATTGCAGAACTGCGAATACAGGATGATTTCAGTTCAGATGATGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04320 map04320
Pfam Domains
Protein Families
FH2

Protein Analysis

992

Amino Acids

108.45

Weight (kDa)

8.72

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 430 - 828 4e-102 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 163, 1471
Acc65I GGTACC 3 cut(s) 180, 1895, 2632
AccB1I GGYRCC 4 cut(s) 180, 1618, 1895, 2632
AccB7I CCANNNNNTGG 1 cut(s) 1211
AccBSI CCGCTC 1 cut(s) 1690
AclI AACGTT 1 cut(s) 113
AclWI GGATC 1 cut(s) 1381
AcoI YGGCCR 2 cut(s) 465, 714
AcsI RAATTY 5 cut(s) 1018, 1224, 2089, 2542, 2883
AcuI CTGAAG 6 cut(s) 1611, 1661, 2129, 2697, 2733, 2805
AcyI GRCGYC 1 cut(s) 93
AfiI CCNNNNNNNGG 5 cut(s) 1112, 1136, 1211, 1235, 2017
AflII CTTAAG 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 2360
AjnI CCWGG 4 cut(s) 517, 1111, 1135, 2546
AloI GAACNNNNNNTCC 2 cut(s) 1452, 1484
Alw21I GWGCWC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
Alw26I GTCTC 5 cut(s) 1410, 1780, 2158, 2256, 2966
Alw44I GTGCAC 2 cut(s) 1911, 2319
AlwI GGATC 1 cut(s) 1381
AlwNI CAGNNNCTG 2 cut(s) 500, 524
Ama87I CYCGRG 1 cut(s) 2259
AoxI GGCC 6 cut(s) 81, 465, 714, 923, 1560, 1678
ApaLI GTGCAC 2 cut(s) 1911, 2319
ApoI RAATTY 5 cut(s) 1018, 1224, 2089, 2542, 2883
AseI ATTAAT 1 cut(s) 200
Asp700I GAANNNNTTC 2 cut(s) 1048, 2448
Asp718I GGTACC 3 cut(s) 180, 1895, 2632
AspLEI GCGC 1 cut(s) 1440
AspS9I GGNCC 8 cut(s) 81, 102, 521, 758, 1082, 1139, 1561, 1679
AsuHPI GGTGA 7 cut(s) 16, 137, 612, 618, 624, 1673, 2120
AvaI CYCGRG 1 cut(s) 2259
AvaII GGWCC 5 cut(s) 102, 521, 758, 1082, 1139
BaeGI GKGCMC 2 cut(s) 1915, 2323
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 4 cut(s) 180, 1618, 1895, 2632
BanII GRGCYC 2 cut(s) 1365, 1596
BbsI GAAGAC 5 cut(s) 2135, 2307, 2357, 2543, 2708
Bbv12I GWGCWC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
BceAI ACGGC 2 cut(s) 1847, 2424
BciT130I CCWGG 4 cut(s) 519, 1113, 1137, 2548
BclI TGATCA 3 cut(s) 411, 789, 1339
BcoDI GTCTC 5 cut(s) 1410, 1780, 2158, 2256, 2966
BfaI CTAG 8 cut(s) 381, 512, 540, 810, 1676, 2324, 2369, 2646
BfmI CTRYAG 4 cut(s) 582, 816, 1683, 2820
BfrI CTTAAG 1 cut(s) 349
BfuAI ACCTGC 2 cut(s) 163, 1471
BglII AGATCT 1 cut(s) 226
Bme1390I CCNGG 4 cut(s) 519, 1113, 1137, 2548
Bme18I GGWCC 5 cut(s) 102, 521, 758, 1082, 1139
BmeT110I CYCGRG 1 cut(s) 2259
BmgT120I GGNCC 8 cut(s) 81, 102, 521, 758, 1082, 1139, 1561, 1679
BmrFI CCNGG 4 cut(s) 519, 1113, 1137, 2548
BmsI GCATC 4 cut(s) 9, 289, 1261, 2194
BpiI GAAGAC 5 cut(s) 2135, 2307, 2357, 2543, 2708
BplI GAGNNNNNCTC 2 cut(s) 1760, 1792
BpmI CTGGAG 4 cut(s) 501, 1095, 2056, 2357
Bpu10I CCTNAGC 3 cut(s) 1646, 1826, 2057
Bsa29I ATCGAT 1 cut(s) 1346
BsaAI YACGTR 1 cut(s) 2471
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 1780
BsaJI CCNNGG 3 cut(s) 1704, 1903, 2137
BsaWI WCCGGW 1 cut(s) 1881
Bsc4I CCNNNNNNNGG 5 cut(s) 1112, 1136, 1211, 1235, 2017
Bse118I RCCGGY 1 cut(s) 712
Bse3DI GCAATG 3 cut(s) 184, 2439, 2529
BseBI CCWGG 4 cut(s) 519, 1113, 1137, 2548
BseCI ATCGAT 1 cut(s) 1346
BseDI CCNNGG 3 cut(s) 1704, 1903, 2137
BseLI CCNNNNNNNGG 5 cut(s) 1112, 1136, 1211, 1235, 2017
BseMI GCAATG 3 cut(s) 184, 2439, 2529
BseMII CTCAG 7 cut(s) 99, 1047, 1548, 1840, 2048, 2169, 2496
BseRI GAGGAG 8 cut(s) 158, 161, 1098, 1116, 1146, 1406, 1586, 1784
BseSI GKGCMC 2 cut(s) 1915, 2323
BsgI GTGCAG 1 cut(s) 2364
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 6 cut(s) 83, 467, 716, 925, 1562, 1680
BshNI GGYRCC 4 cut(s) 180, 1618, 1895, 2632
BshVI ATCGAT 1 cut(s) 1346
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
BsiHKCI CYCGRG 1 cut(s) 2259
BsiSI CCGG 2 cut(s) 713, 1882
BslFI GGGAC 2 cut(s) 223, 2559
BslI CCNNNNNNNGG 5 cut(s) 1112, 1136, 1211, 1235, 2017
BsmAI GTCTC 5 cut(s) 1410, 1780, 2158, 2256, 2966
BsmFI GGGAC 2 cut(s) 223, 2559
BsmI GAATGC 2 cut(s) 2448, 2520
BsnI GGCC 6 cut(s) 83, 467, 716, 925, 1562, 1680
Bso31I GGTCTC 1 cut(s) 1780
BsoBI CYCGRG 1 cut(s) 2259
Bsp1286I GDGCHC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
Bsp143I GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
Bsp19I CCATGG 1 cut(s) 1704
BspANI GGCC 6 cut(s) 83, 467, 716, 925, 1562, 1680
BspCNI CTCAG 7 cut(s) 98, 1048, 1549, 1839, 2049, 2168, 2497
BspDI ATCGAT 1 cut(s) 1346
BspMAI CTGCAG 3 cut(s) 820, 1687, 2824
BspMI ACCTGC 2 cut(s) 163, 1471
BspPI GGATC 1 cut(s) 1381
BspQI GCTCTTC 4 cut(s) 25, 1754, 1826, 2396
BspT107I GGYRCC 4 cut(s) 180, 1618, 1895, 2632
BspTI CTTAAG 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 1780
BsrBI CCGCTC 1 cut(s) 1690
BsrDI GCAATG 3 cut(s) 184, 2439, 2529
BsrFI RCCGGY 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 1704, 1903, 2137
BssMI GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 1704, 2137
Bst2UI CCWGG 4 cut(s) 519, 1113, 1137, 2548
Bst4CI ACNGT 5 cut(s) 271, 1256, 1904, 2227, 2877
Bst6I CTCTTC 8 cut(s) 25, 933, 1164, 1626, 1754, 1826, 2094, 2396
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 349
BstBAI YACGTR 1 cut(s) 2471
BstDEI CTNAG 8 cut(s) 85, 1056, 1557, 1646, 1826, 2057, 2155, 2505
BstDSI CCRYGG 2 cut(s) 1704, 1903
BstENI CCTNNNNNAGG 1 cut(s) 1110
BstHHI GCGC 1 cut(s) 1440
BstKTI GATC 8 cut(s) 229, 414, 792, 852, 1342, 1376, 1666, 2007
BstMAI GTCTC 5 cut(s) 1410, 1780, 2158, 2256, 2966
BstMBI GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 7 cut(s) 41, 497, 713, 743, 815, 2039, 2819
BstNI CCWGG 4 cut(s) 519, 1113, 1137, 2548
BstNSI RCATGY 2 cut(s) 2065, 2364
BstSCI CCNGG 4 cut(s) 517, 1111, 1135, 2546
BstSFI CTRYAG 4 cut(s) 582, 816, 1683, 2820
BstSLI GKGCMC 2 cut(s) 1915, 2323
BstV2I GAAGAC 5 cut(s) 2135, 2307, 2357, 2543, 2708
BstX2I RGATCY 1 cut(s) 226
BstXI CCANNNNNNTGG 2 cut(s) 1314, 2335
BstYI RGATCY 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 1346
BsuRI GGCC 6 cut(s) 83, 467, 716, 925, 1562, 1680
BsuTUI ATCGAT 1 cut(s) 1346
BtgI CCRYGG 2 cut(s) 1704, 1903
BtgZI GCGATG 1 cut(s) 2181
BtsI GCAGTG 1 cut(s) 1742
BtsIMutI CAGTG 8 cut(s) 265, 276, 475, 1261, 1742, 2673, 2709, 2781
BveI ACCTGC 2 cut(s) 163, 1471
CaiI CAGNNNCTG 2 cut(s) 500, 524
CfoI GCGC 1 cut(s) 1440
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 8 cut(s) 81, 102, 521, 758, 1082, 1139, 1561, 1679
ClaI ATCGAT 1 cut(s) 1346
CseI GACGC 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 9 cut(s) 140, 374, 1358, 1705, 1753, 2023, 2062, 2361, 2423
DdeI CTNAG 8 cut(s) 85, 1056, 1557, 1646, 1826, 2057, 2155, 2505
DpnI GATC 8 cut(s) 228, 413, 791, 851, 1341, 1375, 1665, 2006
DpnII GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
EaeI YGGCCR 2 cut(s) 465, 714
Eam1104I CTCTTC 8 cut(s) 25, 933, 1164, 1626, 1754, 1826, 2094, 2396
EarI CTCTTC 8 cut(s) 25, 933, 1164, 1626, 1754, 1826, 2094, 2396
EciI GGCGGA 3 cut(s) 182, 1020, 1119
Ecl136II GAGCTC 2 cut(s) 1363, 1594
Eco130I CCWWGG 2 cut(s) 1704, 2137
Eco147I AGGCCT 1 cut(s) 925
Eco24I GRGCYC 2 cut(s) 1365, 1596
Eco31I GGTCTC 1 cut(s) 1780
Eco47I GGWCC 5 cut(s) 102, 521, 758, 1082, 1139
Eco53kI GAGCTC 2 cut(s) 1363, 1594
Eco57I CTGAAG 6 cut(s) 1611, 1661, 2129, 2697, 2733, 2805
Eco88I CYCGRG 1 cut(s) 2259
EcoICRI GAGCTC 2 cut(s) 1363, 1594
EcoNI CCTNNNNNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 4 cut(s) 81, 521, 1561, 1679
EcoRI GAATTC 1 cut(s) 2542
EcoRII CCWGG 4 cut(s) 517, 1111, 1135, 2546
EcoT14I CCWWGG 2 cut(s) 1704, 2137
EcoT38I GRGCYC 2 cut(s) 1365, 1596
ErhI CCWWGG 2 cut(s) 1704, 2137
FaeI CATG 9 cut(s) 143, 377, 1361, 1708, 1756, 2026, 2065, 2364, 2426
FaqI GGGAC 2 cut(s) 223, 2559
FatI CATG 9 cut(s) 139, 373, 1357, 1704, 1752, 2022, 2061, 2360, 2422
FauI CCCGC 3 cut(s) 200, 1179, 2042
FauNDI CATATG 1 cut(s) 162
FbaI TGATCA 3 cut(s) 411, 789, 1339
FriOI GRGCYC 2 cut(s) 1365, 1596
FspBI CTAG 8 cut(s) 381, 512, 540, 810, 1676, 2324, 2369, 2646
GlaI GCGC 1 cut(s) 1439
GsuI CTGGAG 4 cut(s) 501, 1095, 2056, 2357
HaeIII GGCC 6 cut(s) 83, 467, 716, 925, 1562, 1680
HapII CCGG 2 cut(s) 713, 1882
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 1440
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 9 cut(s) 143, 377, 1361, 1708, 1756, 2026, 2065, 2364, 2426
Hin6I GCGC 1 cut(s) 1438
HinP1I GCGC 1 cut(s) 1438
HindIII AAGCTT 1 cut(s) 1643
HpaII CCGG 2 cut(s) 713, 1882
HphI GGTGA 7 cut(s) 16, 137, 612, 618, 624, 1673, 2120
Hpy166II GTNNAC 8 cut(s) 662, 701, 731, 761, 908, 1913, 2272, 2321
Hpy8I GTNNAC 8 cut(s) 662, 701, 731, 761, 908, 1913, 2272, 2321
Hpy99I CGWCG 1 cut(s) 2913
HpyCH4III ACNGT 5 cut(s) 271, 1256, 1904, 2227, 2877
HpyCH4IV ACGT 3 cut(s) 113, 559, 2470
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 497, 713, 743, 815, 2039, 2819
HpyF3I CTNAG 8 cut(s) 85, 1056, 1557, 1646, 1826, 2057, 2155, 2505
HpySE526I ACGT 3 cut(s) 113, 559, 2470
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 9 cut(s) 143, 377, 1361, 1708, 1756, 2026, 2065, 2364, 2426
HspAI GCGC 1 cut(s) 1438
KpnI GGTACC 3 cut(s) 184, 1899, 2636
Ksp22I TGATCA 3 cut(s) 411, 789, 1339
Kzo9I GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
LguI GCTCTTC 4 cut(s) 25, 1754, 1826, 2396
LweI GCATC 4 cut(s) 9, 289, 1261, 2194
MaeI CTAG 8 cut(s) 381, 512, 540, 810, 1676, 2324, 2369, 2646
MaeII ACGT 3 cut(s) 113, 559, 2470
MaeIII GTNAC 3 cut(s) 260, 1537, 2132
MalI GATC 8 cut(s) 228, 413, 791, 851, 1341, 1375, 1665, 2006
MbiI CCGCTC 1 cut(s) 1690
MboI GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
MfeI CAATTG 1 cut(s) 2928
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
MlsI TGGCCA 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 5 cut(s) 599, 1412, 2386, 2698, 2910
MmeI TCCRAC 2 cut(s) 2091, 2840
Mox20I TGGCCA 1 cut(s) 467
MroXI GAANNNNTTC 2 cut(s) 1048, 2448
MscI TGGCCA 1 cut(s) 467
MseI TTAA 9 cut(s) 200, 350, 434, 894, 1301, 1326, 1728, 2162, 2216
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 2 cut(s) 713, 1882
MspR9I CCNGG 4 cut(s) 519, 1113, 1137, 2548
MunI CAATTG 1 cut(s) 2928
Mva1269I GAATGC 2 cut(s) 2448, 2520
MvaI CCWGG 4 cut(s) 519, 1113, 1137, 2548
MwoI GCNNNNNNNGC 7 cut(s) 41, 497, 713, 743, 815, 2039, 2819
NcoI CCATGG 1 cut(s) 1704
NdeI CATATG 1 cut(s) 162
NdeII GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
NlaIII CATG 9 cut(s) 143, 377, 1361, 1708, 1756, 2026, 2065, 2364, 2426
NmuCI GTSAC 2 cut(s) 260, 1537
NspI RCATGY 2 cut(s) 2065, 2364
PaeR7I CTCGAG 1 cut(s) 2259
PceI AGGCCT 1 cut(s) 925
PciI ACATGT 1 cut(s) 2360
PciSI GCTCTTC 4 cut(s) 25, 1754, 1826, 2396
PctI GAATGC 2 cut(s) 2448, 2520
PdmI GAANNNNTTC 2 cut(s) 1048, 2448
PfeI GAWTC 5 cut(s) 55, 1049, 1283, 1790, 2048
PflMI CCANNNNNTGG 1 cut(s) 1211
PfoI TCCNGGA 3 cut(s) 1111, 1135, 2546
PleI GAGTC 5 cut(s) 598, 1411, 2386, 2698, 2910
PpsI GAGTC 5 cut(s) 598, 1411, 2386, 2698, 2910
Ppu21I YACGTR 1 cut(s) 2471
PpuMI RGGWCCY 1 cut(s) 521
PscI ACATGT 1 cut(s) 2360
PshBI ATTAAT 1 cut(s) 200
Psp124BI GAGCTC 2 cut(s) 1365, 1596
Psp1406I AACGTT 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 4 cut(s) 517, 1111, 1135, 2546
PspGI CCWGG 4 cut(s) 517, 1111, 1135, 2546
PspPI GGNCC 8 cut(s) 81, 102, 521, 758, 1082, 1139, 1561, 1679
PspPPI RGGWCCY 1 cut(s) 521
PsrI GAACNNNNNNTAC 2 cut(s) 2928, 2960
PstI CTGCAG 3 cut(s) 820, 1687, 2824
PstNI CAGNNNCTG 2 cut(s) 500, 524
PsuI RGATCY 1 cut(s) 226
SacI GAGCTC 2 cut(s) 1365, 1596
SapI GCTCTTC 4 cut(s) 25, 1754, 1826, 2396
SaqAI TTAA 9 cut(s) 200, 350, 434, 894, 1301, 1326, 1728, 2162, 2216
Sau3AI GATC 8 cut(s) 226, 411, 789, 849, 1339, 1373, 1663, 2004
Sau96I GGNCC 8 cut(s) 81, 102, 521, 758, 1082, 1139, 1561, 1679
SchI GAGTC 5 cut(s) 599, 1412, 2386, 2698, 2910
ScrFI CCNGG 4 cut(s) 519, 1113, 1137, 2548
SduI GDGCHC 7 cut(s) 553, 1365, 1596, 1915, 2127, 2323, 2408
SfaNI GCATC 4 cut(s) 9, 289, 1261, 2194
SfcI CTRYAG 4 cut(s) 582, 816, 1683, 2820
Sfr274I CTCGAG 1 cut(s) 2259
SinI GGWCC 5 cut(s) 102, 521, 758, 1082, 1139
SlaI CTCGAG 1 cut(s) 2259
SmlI CTYRAG 2 cut(s) 349, 2259
SmoI CTYRAG 2 cut(s) 349, 2259
SseBI AGGCCT 1 cut(s) 925
SspI AATATT 1 cut(s) 2236
SspMI CTAG 8 cut(s) 381, 512, 540, 810, 1676, 2324, 2369, 2646
SstI GAGCTC 2 cut(s) 1365, 1596
StuI AGGCCT 1 cut(s) 925
StyD4I CCNGG 4 cut(s) 517, 1111, 1135, 2546
StyI CCWWGG 2 cut(s) 1704, 2137
TaaI ACNGT 5 cut(s) 271, 1256, 1904, 2227, 2877
TaiI ACGT 3 cut(s) 116, 562, 2473
TaqI TCGA 3 cut(s) 547, 1346, 2260
TatI WGTACW 1 cut(s) 841
TauI GCSGC 2 cut(s) 399, 500
TfiI GAWTC 5 cut(s) 55, 1049, 1283, 1790, 2048
Tru1I TTAA 9 cut(s) 200, 350, 434, 894, 1301, 1326, 1728, 2162, 2216
Tru9I TTAA 9 cut(s) 200, 350, 434, 894, 1301, 1326, 1728, 2162, 2216
TscAI CASTG 8 cut(s) 265, 276, 475, 1261, 1749, 2680, 2716, 2788
TseFI GTSAC 2 cut(s) 260, 1537
Tsp45I GTSAC 2 cut(s) 260, 1537
TspGWI ACGGA 3 cut(s) 1559, 2000, 2099
TspRI CASTG 8 cut(s) 265, 276, 475, 1261, 1749, 2680, 2716, 2788
Van91I CCANNNNNTGG 1 cut(s) 1211
Vha464I CTTAAG 1 cut(s) 349
VneI GTGCAC 2 cut(s) 1911, 2319
VpaK11BI GGWCC 5 cut(s) 102, 521, 758, 1082, 1139
VspI ATTAAT 1 cut(s) 200
XagI CCTNNNNNAGG 1 cut(s) 1110
XapI RAATTY 5 cut(s) 1018, 1224, 2089, 2542, 2883
XbaI TCTAGA 1 cut(s) 2368
XceI RCATGY 2 cut(s) 2065, 2364
XhoI CTCGAG 1 cut(s) 2259
XmnI GAANNNNTTC 2 cut(s) 1048, 2448
XspI CTAG 8 cut(s) 381, 512, 540, 810, 1676, 2324, 2369, 2646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.