Rroxscaffold_2G00089860

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011587
Physical Location & Seq
Reverse (-)
11761524 .. 11766789
5266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089860.1

Sequence Viewer

Length: 2820 bp
ATGAGAAGAGCCAGTTATGCGGTTTTCGTGATTCTGCTCTGTGCATTGGTCATTGGGGCCTCAGAGGGCGTCAGGAGGACCAAAGAAACGTTTTATGGAGGTGATTGGGTTTCGCATGAGGAGGAGATAGATGAACATATGGCGGAGCAGGTATGGTACCATTGCAGGAAAGAACTAATAGGCGGGACAGATGGGGTGGAAGATCTTGACTTGTACATTCCACAGGAAGCAGCCAGTGACTCGGAAACAGTGTTCTTTTCAAAAAGCAACATGCACGATGCGATTTTAGTTCTTCCTCCTGATGTGAAACAAACACTTGCTGATTGCTTAAGGAAGAAGGGGATTGTGTTTCATGCTCCTGGCAAAAGGGATAGCGGCAACAACTGGTTGATCAACTACTTTGAGTGGCTTTTAAGTTCGGATATTGCTCCTAGAAGATATTTGGCCAGTGGTTCCCCCAAGTCAAAAGCACCAGCCGCTGCTCCATCTCTAGCTCCAGGTCCTGCATCTAAGTTGTCTAGCGGTCGAGCACCTCCACGTTCAGTTCTATCTCCAAGCCCTACAGATGAGTCAGATAACAATGAAGCACCAGCACCTTCACCTTCACCTCCTAAACAAAGTGGGAAGGGACGTCCACCTCCCAAACCAAGTGGGAAAGAAGCTGCTTCAAGTCCACCAGCAAAACCGGCCAAAACAAAAAGTCCACCAGCAAAACCAGCCCAAACAAATGGTCCACCATCAAAGGATGGCAGTATGAGTTCTTCACAAATATCCAATAGTTTAGGTTCAAGTACTAGAGAGTTCAACGCTGGTGGAAAGTTGGGAAATTTGCCTATGAAAAATGAAAAAGGAAATGATTCTTCTGAGGCAAAGTCAGAAGGTACAGCAGGACCACCAAACCCTCCTGTACCACCTCCTCCAGGAAAAGCAGCTCCTCCGCCTCCTGGACCACCACCACCGCCTCCTCTTCGTCCTCGCCCGCCACCAGTACCTCCCAAAAATGCACCTAAACCCATGGCTGGTAAGACCCAAATTCCACCTAAAGGACATCTCAAAGCAAACAGTGGCGATAATGCTGACGCTGGAAGTGAATCTGGAAAGACCAAGTTAAAACCATTCTTCTGGGATAAGGTTAATGCCAACCCTGATCAATCGATGGTCTGGCATGAGCTCAAAGAAGGATCATTCCAGTTCAATGAGGAGAAGATAGAGTCTCTATTTGGTTATACAAAGAGCCAAAATGAGCGCAAAAAGGTTTCACCATCGGAACCTGCAATCCAGTTTATCCAAATTATTGACAAAAAGAAAGCACAAAATCTATCAATTCTTCTGCGAGCATTGAATGTGACAACAGAAGAAGTTGCTGAGGCCCTCCGAGAAGGCATCATCGACTTACATCTATTCACTGAATCAGGCAACGAGCTTCCTGTGGAGCTCCTCCAAACCCTGTTGAAGATGGCACCAACTTCAGAAGAGGAACTGAAGCTTAGGTTATACACAGGTGATCTTGCTCTACTAGGCCCTGCAGAGCGGTTCCTCAAAGTCATGGTTGAGATACCGTTTGCTTTTAAGAGGATGGAGGCACTGCTCTTCATGTGTTCCTTCAATGAGGAGGTCTCTAACACTAGAGAATCCTTTGCAACTTTAGAGGTTGCTTGCAGCAAGCTCAGGAAGAGTAGGCTATTCCTAAAACTTTTGGAAGCCGTACTAAAAACTGGCAACCGGATGAATGACGGTACCTACCGTGGTGGTGCACAAGCATTTAGGCTTGATACGCTCTTGAAACTAGCTGATGTAAAAGGAACTGATGGCAAGACCACACTCTTGCACTTTGTCGTTCAAGAGATCATCCGTTATGAGGGCATGAAAGCTGCCCGCAGAGCCAGAGAATCAAACCGGAGCATGTCCAACTCCAGTATGAGTACAGAGGAGTTTGTTGAAGAGGTTGGTGAGGAATCAGCAGAGCACTATCGTAACCTTGGTCTTCAGGTTGTCTCAGGTTTAAGCGATGAACTTCAAGATGTGAAAAAGGCAGCAGTTGTAGATGCCGATAGCTTAACTGCAACAGTTTCAAATATTGGCACAAATCTAATAAAAGCTCGAGACTTTGTAAACACCGACTTGAAGAATAATCTGGATGAAGACAGCGAGTTCCATCATGCACTAGCCAGTTTTCTGGAGCGTGCAGAAGGAGAAACTACATTTCTTCTAGAAGAAGAAAAGAGGATAACGACTCTTGTGAAGAGCACGGCAGATTACTTTCATGGGAACGCAATGAAGGATGAAGGCATTCGTTTGTTTGCCATTGTACGTGATTTCTTGGTAATACTAAATAAGGTATGTGCTGAGATTAGAATGCAAGCAATGAAGCAAGCAAAGACGAATTCCAGGAAAGAGGCTCTCACACAAGCAAGTTCCAAGAAGGAGGCTCTCACACAAGCAAATTCAGAAAAGCAGTCAGAAATGAATAAGGTACCCACAGAAGCTAGTTCAGAAAAGAAAGAGAAGGTTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGACTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACAGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACAGAAGCAAGTTCAGAAAAGAAAGAGAAGGCTCCCACTGAAGCAAGTTCAGAAAAGAAAGAGAAGCCTCCCACAGAAGCCAGTTCAGAAAAACCGCGGGAAATTCAACAGCAACATTCAGATATTCGTCGGCGACTCTTTCCAGCAATTGCAGAACTGCGAATAGAGGACGATTTCAGTTCAGATGATGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04320 map04320
Pfam Domains
Protein Families
FH2

Protein Analysis

939

Amino Acids

102.81

Weight (kDa)

6.49

Isoelectric Point (pI)

56.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 366 - 775 3.4e-99 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 634
Acc36I ACCTGC 2 cut(s) 139, 1279
Acc65I GGTACC 3 cut(s) 156, 1736, 2473
AccB1I GGYRCC 4 cut(s) 156, 1459, 1736, 2473
AccBSI CCGCTC 1 cut(s) 1531
AccII CGCG 1 cut(s) 2719
AclI AACGTT 1 cut(s) 89
AclWI GGATC 1 cut(s) 1189
AcoI YGGCCR 2 cut(s) 444, 687
AcsI RAATTY 5 cut(s) 826, 1032, 2383, 2443, 2724
AcuI CTGAAG 6 cut(s) 1452, 1502, 1970, 2538, 2574, 2682
AcyI GRCGYC 2 cut(s) 69, 631
AfiI CCNNNNNNNGG 5 cut(s) 920, 944, 1019, 1043, 1858
AflII CTTAAG 1 cut(s) 328
AjnI CCWGG 5 cut(s) 358, 496, 919, 943, 2387
AloI GAACNNNNNNTCC 4 cut(s) 236, 268, 1260, 1292
Alw21I GWGCWC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
Alw26I GTCTC 5 cut(s) 1218, 1621, 1999, 2097, 2807
Alw44I GTGCAC 1 cut(s) 1752
AlwI GGATC 1 cut(s) 1189
AlwNI CAGNNNCTG 2 cut(s) 479, 503
Ama87I CYCGRG 1 cut(s) 2100
AoxI GGCC 5 cut(s) 57, 444, 687, 1368, 1519
ApaLI GTGCAC 1 cut(s) 1752
ApeKI GCWGC 7 cut(s) 230, 479, 662, 929, 1659, 1871, 2033
ApoI RAATTY 5 cut(s) 826, 1032, 2383, 2443, 2724
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
Asp700I GAANNNNTTC 2 cut(s) 856, 2289
Asp718I GGTACC 3 cut(s) 156, 1736, 2473
AspLEI GCGC 1 cut(s) 1248
AspS9I GGNCC 8 cut(s) 57, 78, 500, 731, 890, 947, 1369, 1520
AsuHPI GGTGA 6 cut(s) 113, 591, 597, 1251, 1514, 1961
AvaI CYCGRG 1 cut(s) 2100
AvaII GGWCC 5 cut(s) 78, 500, 731, 890, 947
BaeGI GKGCMC 1 cut(s) 1756
BalI TGGCCA 1 cut(s) 446
BanI GGYRCC 4 cut(s) 156, 1459, 1736, 2473
BanII GRGCYC 2 cut(s) 1173, 1437
BarI GAAGNNNNNNTAC 2 cut(s) 745, 777
BbsI GAAGAC 3 cut(s) 1976, 2148, 2549
Bbv12I GWGCWC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
BbvCI CCTCAGC 1 cut(s) 1365
BbvI GCAGC 7 cut(s) 242, 466, 649, 941, 1671, 1858, 2045
BceAI ACGGC 2 cut(s) 1688, 2265
BciT130I CCWGG 5 cut(s) 360, 498, 921, 945, 2389
BclI TGATCA 2 cut(s) 390, 1147
BcoDI GTCTC 5 cut(s) 1218, 1621, 1999, 2097, 2807
BfmI CTRYAG 2 cut(s) 561, 1524
BfrI CTTAAG 1 cut(s) 328
BfuAI ACCTGC 2 cut(s) 139, 1279
BglII AGATCT 1 cut(s) 202
BisI GCNGC 9 cut(s) 231, 376, 477, 480, 663, 930, 1660, 1872, 2034
BlsI GCNGC 9 cut(s) 232, 377, 478, 481, 664, 931, 1661, 1873, 2035
BmcAI AGTACT 1 cut(s) 793
Bme1390I CCNGG 5 cut(s) 360, 498, 921, 945, 2389
Bme18I GGWCC 5 cut(s) 78, 500, 731, 890, 947
BmeT110I CYCGRG 1 cut(s) 2100
BmgT120I GGNCC 8 cut(s) 57, 78, 500, 731, 890, 947, 1369, 1520
BmrFI CCNGG 5 cut(s) 360, 498, 921, 945, 2389
BmsI GCATC 4 cut(s) 268, 515, 1392, 2035
BpiI GAAGAC 3 cut(s) 1976, 2148, 2549
BplI GAGNNNNNCTC 2 cut(s) 1601, 1633
BpmI CTGGAG 4 cut(s) 480, 903, 1897, 2198
Bpu10I CCTNAGC 3 cut(s) 1365, 1487, 1667
Bsa29I ATCGAT 1 cut(s) 1154
BsaAI YACGTR 1 cut(s) 2312
BsaHI GRCGYC 2 cut(s) 69, 631
BsaI GGTCTC 1 cut(s) 1621
BsaJI CCNNGG 4 cut(s) 1014, 1744, 1978, 2717
BsaWI WCCGGW 2 cut(s) 1722, 1896
Bsc4I CCNNNNNNNGG 5 cut(s) 920, 944, 1019, 1043, 1858
Bse118I RCCGGY 1 cut(s) 685
Bse3DI GCAATG 3 cut(s) 160, 2280, 2370
BseBI CCWGG 5 cut(s) 360, 498, 921, 945, 2389
BseCI ATCGAT 1 cut(s) 1154
BseDI CCNNGG 4 cut(s) 1014, 1744, 1978, 2717
BseGI GGATG 6 cut(s) 751, 1581, 1731, 1848, 2143, 2287
BseLI CCNNNNNNNGG 5 cut(s) 920, 944, 1019, 1043, 1858
BseMI GCAATG 3 cut(s) 160, 2280, 2370
BseMII CTCAG 6 cut(s) 75, 855, 1356, 1681, 2010, 2337
BseRI GAGGAG 9 cut(s) 134, 137, 906, 924, 954, 1214, 1427, 1625, 1943
BseSI GKGCMC 1 cut(s) 1756
BseXI GCAGC 7 cut(s) 242, 466, 649, 941, 1671, 1858, 2045
BsgI GTGCAG 1 cut(s) 2205
Bsh1236I CGCG 1 cut(s) 2719
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 5 cut(s) 59, 446, 689, 1370, 1521
BshNI GGYRCC 4 cut(s) 156, 1459, 1736, 2473
BshVI ATCGAT 1 cut(s) 1154
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
BsiHKCI CYCGRG 1 cut(s) 2100
BsiSI CCGG 3 cut(s) 686, 1723, 1897
BslFI GGGAC 2 cut(s) 199, 642
BslI CCNNNNNNNGG 5 cut(s) 920, 944, 1019, 1043, 1858
BsmAI GTCTC 5 cut(s) 1218, 1621, 1999, 2097, 2807
BsmFI GGGAC 2 cut(s) 199, 642
BsmI GAATGC 2 cut(s) 2289, 2361
BsnI GGCC 5 cut(s) 59, 446, 689, 1370, 1521
Bso31I GGTCTC 1 cut(s) 1621
BsoBI CYCGRG 1 cut(s) 2100
Bsp1286I GDGCHC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
Bsp19I CCATGG 1 cut(s) 1014
BspANI GGCC 5 cut(s) 59, 446, 689, 1370, 1521
BspCNI CTCAG 6 cut(s) 74, 856, 1357, 1680, 2009, 2338
BspDI ATCGAT 1 cut(s) 1154
BspFNI CGCG 1 cut(s) 2719
BspMAI CTGCAG 1 cut(s) 1528
BspMI ACCTGC 2 cut(s) 139, 1279
BspPI GGATC 1 cut(s) 1189
BspQI GCTCTTC 2 cut(s) 1595, 2237
BspT107I GGYRCC 4 cut(s) 156, 1459, 1736, 2473
BspTI CTTAAG 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 1621
BsrBI CCGCTC 1 cut(s) 1531
BsrDI GCAATG 3 cut(s) 160, 2280, 2370
BsrFI RCCGGY 1 cut(s) 685
BsrGI TGTACA 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 685
BssECI CCNNGG 4 cut(s) 1014, 1744, 1978, 2717
BssMI GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
BssNI GRCGYC 2 cut(s) 69, 631
BssT1I CCWWGG 2 cut(s) 1014, 1978
Bst2UI CCWGG 5 cut(s) 360, 498, 921, 945, 2389
Bst4CI ACNGT 6 cut(s) 250, 1064, 1560, 1736, 1745, 2068
Bst6I CTCTTC 6 cut(s) 972, 1467, 1595, 1667, 1935, 2237
BstACI GRCGYC 2 cut(s) 69, 631
BstAFI CTTAAG 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 2312
BstC8I GCNNGC 8 cut(s) 980, 1335, 1657, 1664, 1876, 2184, 2361, 2373
BstDEI CTNAG 8 cut(s) 61, 510, 864, 1365, 1487, 1667, 1996, 2346
BstDSI CCRYGG 3 cut(s) 1014, 1744, 2717
BstENI CCTNNNNNAGG 1 cut(s) 918
BstF5I GGATG 6 cut(s) 751, 1581, 1731, 1848, 2143, 2287
BstFNI CGCG 1 cut(s) 2719
BstHHI GCGC 1 cut(s) 1248
BstKTI GATC 6 cut(s) 205, 393, 1150, 1184, 1507, 1848
BstMAI GTCTC 5 cut(s) 1218, 1621, 1999, 2097, 2807
BstMBI GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 6 cut(s) 17, 476, 686, 716, 1774, 1880
BstNI CCWGG 5 cut(s) 360, 498, 921, 945, 2389
BstNSI RCATGY 2 cut(s) 274, 1906
BstSCI CCNGG 5 cut(s) 358, 496, 919, 943, 2387
BstSFI CTRYAG 2 cut(s) 561, 1524
BstSLI GKGCMC 1 cut(s) 1756
BstUI CGCG 1 cut(s) 2719
BstV1I GCAGC 7 cut(s) 242, 466, 649, 941, 1671, 1858, 2045
BstV2I GAAGAC 3 cut(s) 1976, 2148, 2549
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 3 cut(s) 728, 1122, 2176
BstYI RGATCY 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 1154
BsuRI GGCC 5 cut(s) 59, 446, 689, 1370, 1521
BsuTUI ATCGAT 1 cut(s) 1154
BtgI CCRYGG 3 cut(s) 1014, 1744, 2717
BtgZI GCGATG 1 cut(s) 2022
BtsCI GGATG 6 cut(s) 751, 1581, 1731, 1848, 2143, 2287
BtsI GCAGTG 1 cut(s) 1583
BtsIMutI CAGTG 9 cut(s) 241, 255, 454, 1069, 1404, 1583, 2514, 2550, 2658
BveI ACCTGC 2 cut(s) 139, 1279
Cac8I GCNNGC 8 cut(s) 980, 1335, 1657, 1664, 1876, 2184, 2361, 2373
CaiI CAGNNNCTG 2 cut(s) 479, 503
CfoI GCGC 1 cut(s) 1248
Cfr10I RCCGGY 1 cut(s) 685
Cfr13I GGNCC 8 cut(s) 57, 78, 500, 731, 890, 947, 1369, 1520
Cfr42I CCGCGG 1 cut(s) 2720
ClaI ATCGAT 1 cut(s) 1154
CseI GACGC 2 cut(s) 58, 1088
CspCI CAANNNNNGTGG 4 cut(s) 631, 666, 793, 828
DdeI CTNAG 8 cut(s) 61, 510, 864, 1365, 1487, 1667, 1996, 2346
DpnI GATC 6 cut(s) 204, 392, 1149, 1183, 1506, 1847
DpnII GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
EaeI YGGCCR 2 cut(s) 444, 687
Eam1104I CTCTTC 6 cut(s) 972, 1467, 1595, 1667, 1935, 2237
EarI CTCTTC 6 cut(s) 972, 1467, 1595, 1667, 1935, 2237
EciI GGCGGA 2 cut(s) 158, 927
Ecl136II GAGCTC 2 cut(s) 1171, 1435
Eco130I CCWWGG 2 cut(s) 1014, 1978
Eco24I GRGCYC 2 cut(s) 1173, 1437
Eco31I GGTCTC 1 cut(s) 1621
Eco47I GGWCC 5 cut(s) 78, 500, 731, 890, 947
Eco53kI GAGCTC 2 cut(s) 1171, 1435
Eco57I CTGAAG 6 cut(s) 1452, 1502, 1970, 2538, 2574, 2682
Eco88I CYCGRG 1 cut(s) 2100
EcoICRI GAGCTC 2 cut(s) 1171, 1435
EcoNI CCTNNNNNAGG 1 cut(s) 918
EcoO109I RGGNCCY 4 cut(s) 57, 500, 1369, 1520
EcoRI GAATTC 1 cut(s) 2383
EcoRII CCWGG 5 cut(s) 358, 496, 919, 943, 2387
EcoT14I CCWWGG 2 cut(s) 1014, 1978
EcoT38I GRGCYC 2 cut(s) 1173, 1437
ErhI CCWWGG 2 cut(s) 1014, 1978
FaqI GGGAC 2 cut(s) 199, 642
FauI CCCGC 4 cut(s) 176, 987, 1883, 2712
FauNDI CATATG 1 cut(s) 138
FbaI TGATCA 2 cut(s) 390, 1147
Fnu4HI GCNGC 9 cut(s) 231, 376, 477, 480, 663, 930, 1660, 1872, 2034
FokI GGATG 6 cut(s) 758, 1588, 1738, 1835, 2150, 2294
FriOI GRGCYC 2 cut(s) 1173, 1437
Fsp4HI GCNGC 9 cut(s) 231, 376, 477, 480, 663, 930, 1660, 1872, 2034
GlaI GCGC 1 cut(s) 1247
GluI GCNGC 9 cut(s) 231, 376, 477, 480, 663, 930, 1660, 1872, 2034
GsuI CTGGAG 4 cut(s) 480, 903, 1897, 2198
HaeIII GGCC 5 cut(s) 59, 446, 689, 1370, 1521
HapII CCGG 3 cut(s) 686, 1723, 1897
HgaI GACGC 2 cut(s) 58, 1088
HhaI GCGC 1 cut(s) 1248
Hin1I GRCGYC 2 cut(s) 69, 631
Hin6I GCGC 1 cut(s) 1246
HinP1I GCGC 1 cut(s) 1246
HindIII AAGCTT 1 cut(s) 1484
HpaII CCGG 3 cut(s) 686, 1723, 1897
HphI GGTGA 6 cut(s) 113, 591, 597, 1251, 1514, 1961
Hpy166II GTNNAC 6 cut(s) 635, 674, 704, 734, 1754, 2113
Hpy8I GTNNAC 6 cut(s) 635, 674, 704, 734, 1754, 2113
Hpy99I CGWCG 1 cut(s) 2754
HpyCH4III ACNGT 6 cut(s) 250, 1064, 1560, 1736, 1745, 2068
HpyCH4IV ACGT 4 cut(s) 89, 538, 631, 2311
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 476, 686, 716, 1774, 1880
HpyF3I CTNAG 8 cut(s) 61, 510, 864, 1365, 1487, 1667, 1996, 2346
HpySE526I ACGT 4 cut(s) 89, 538, 631, 2311
Hsp92I GRCGYC 2 cut(s) 69, 631
HspAI GCGC 1 cut(s) 1246
KpnI GGTACC 3 cut(s) 160, 1740, 2477
Ksp22I TGATCA 2 cut(s) 390, 1147
KspI CCGCGG 1 cut(s) 2720
Kzo9I GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
LguI GCTCTTC 2 cut(s) 1595, 2237
Lsp1109I GCAGC 7 cut(s) 242, 466, 649, 941, 1671, 1858, 2045
LweI GCATC 4 cut(s) 268, 515, 1392, 2035
MaeII ACGT 4 cut(s) 89, 538, 631, 2311
MaeIII GTNAC 3 cut(s) 236, 1345, 1973
MalI GATC 6 cut(s) 204, 392, 1149, 1183, 1506, 1847
MbiI CCGCTC 1 cut(s) 1531
MboI GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
MfeI CAATTG 1 cut(s) 2769
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 8 cut(s) 826, 1032, 1290, 1323, 2383, 2443, 2724, 2769
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 6 cut(s) 233, 578, 1220, 2227, 2539, 2751
MmeI TCCRAC 1 cut(s) 1932
Mox20I TGGCCA 1 cut(s) 446
MroXI GAANNNNTTC 2 cut(s) 856, 2289
MscI TGGCCA 1 cut(s) 446
MseI TTAA 7 cut(s) 329, 413, 1109, 1134, 1569, 2003, 2057
Msp20I TGGCCA 1 cut(s) 446
MspA1I CMGCKG 2 cut(s) 479, 2719
MspCI CTTAAG 1 cut(s) 328
MspI CCGG 3 cut(s) 686, 1723, 1897
MspR9I CCNGG 5 cut(s) 360, 498, 921, 945, 2389
MunI CAATTG 1 cut(s) 2769
Mva1269I GAATGC 2 cut(s) 2289, 2361
MvaI CCWGG 5 cut(s) 360, 498, 921, 945, 2389
MvnI CGCG 1 cut(s) 2719
MwoI GCNNNNNNNGC 6 cut(s) 17, 476, 686, 716, 1774, 1880
NcoI CCATGG 1 cut(s) 1014
NdeI CATATG 1 cut(s) 138
NdeII GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
NmuCI GTSAC 2 cut(s) 236, 1345
NspI RCATGY 2 cut(s) 274, 1906
PaeR7I CTCGAG 1 cut(s) 2100
PciSI GCTCTTC 2 cut(s) 1595, 2237
PctI GAATGC 2 cut(s) 2289, 2361
PdmI GAANNNNTTC 2 cut(s) 856, 2289
PfeI GAWTC 7 cut(s) 31, 857, 1091, 1409, 1631, 1889, 1955
PfoI TCCNGGA 3 cut(s) 919, 943, 2387
PkrI GCNGC 9 cut(s) 232, 377, 478, 481, 664, 931, 1661, 1873, 2035
PleI GAGTC 6 cut(s) 233, 577, 1219, 2227, 2539, 2751
PpsI GAGTC 6 cut(s) 233, 577, 1219, 2227, 2539, 2751
Ppu21I YACGTR 1 cut(s) 2312
PpuMI RGGWCCY 1 cut(s) 500
Psp124BI GAGCTC 2 cut(s) 1173, 1437
Psp1406I AACGTT 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 5 cut(s) 358, 496, 919, 943, 2387
PspGI CCWGG 5 cut(s) 358, 496, 919, 943, 2387
PspPI GGNCC 8 cut(s) 57, 78, 500, 731, 890, 947, 1369, 1520
PspPPI RGGWCCY 1 cut(s) 500
PstI CTGCAG 1 cut(s) 1528
PstNI CAGNNNCTG 2 cut(s) 479, 503
PsuI RGATCY 1 cut(s) 202
SacI GAGCTC 2 cut(s) 1173, 1437
SacII CCGCGG 1 cut(s) 2720
SapI GCTCTTC 2 cut(s) 1595, 2237
SaqAI TTAA 7 cut(s) 329, 413, 1109, 1134, 1569, 2003, 2057
SatI GCNGC 9 cut(s) 231, 376, 477, 480, 663, 930, 1660, 1872, 2034
Sau3AI GATC 6 cut(s) 202, 390, 1147, 1181, 1504, 1845
Sau96I GGNCC 8 cut(s) 57, 78, 500, 731, 890, 947, 1369, 1520
ScaI AGTACT 1 cut(s) 793
SchI GAGTC 6 cut(s) 233, 578, 1220, 2227, 2539, 2751
ScrFI CCNGG 5 cut(s) 360, 498, 921, 945, 2389
SduI GDGCHC 6 cut(s) 532, 1173, 1437, 1756, 1968, 2249
SfaNI GCATC 4 cut(s) 268, 515, 1392, 2035
SfcI CTRYAG 2 cut(s) 561, 1524
Sfr274I CTCGAG 1 cut(s) 2100
Sfr303I CCGCGG 1 cut(s) 2720
SgrBI CCGCGG 1 cut(s) 2720
SinI GGWCC 5 cut(s) 78, 500, 731, 890, 947
SlaI CTCGAG 1 cut(s) 2100
SmlI CTYRAG 2 cut(s) 328, 2100
SmoI CTYRAG 2 cut(s) 328, 2100
Sse9I AATT 8 cut(s) 826, 1032, 1290, 1323, 2383, 2443, 2724, 2769
SspI AATATT 1 cut(s) 2077
SstI GAGCTC 2 cut(s) 1173, 1437
StyD4I CCNGG 5 cut(s) 358, 496, 919, 943, 2387
StyI CCWWGG 2 cut(s) 1014, 1978
TaaI ACNGT 6 cut(s) 250, 1064, 1560, 1736, 1745, 2068
TaiI ACGT 4 cut(s) 92, 541, 634, 2314
TaqI TCGA 4 cut(s) 526, 1154, 1389, 2101
TasI AATT 8 cut(s) 826, 1032, 1290, 1323, 2383, 2443, 2724, 2769
TatI WGTACW 3 cut(s) 213, 791, 1922
TauI GCSGC 2 cut(s) 378, 479
TfiI GAWTC 7 cut(s) 31, 857, 1091, 1409, 1631, 1889, 1955
Tru1I TTAA 7 cut(s) 329, 413, 1109, 1134, 1569, 2003, 2057
Tru9I TTAA 7 cut(s) 329, 413, 1109, 1134, 1569, 2003, 2057
TscAI CASTG 9 cut(s) 241, 255, 454, 1069, 1411, 1590, 2521, 2557, 2665
TseFI GTSAC 2 cut(s) 236, 1345
TseI GCWGC 7 cut(s) 230, 479, 662, 929, 1659, 1871, 2033
Tsp45I GTSAC 2 cut(s) 236, 1345
TspGWI ACGGA 1 cut(s) 1841
TspRI CASTG 9 cut(s) 241, 255, 454, 1069, 1411, 1590, 2521, 2557, 2665
Vha464I CTTAAG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 1752
VpaK11BI GGWCC 5 cut(s) 78, 500, 731, 890, 947
XagI CCTNNNNNAGG 1 cut(s) 918
XapI RAATTY 5 cut(s) 826, 1032, 2383, 2443, 2724
XbaI TCTAGA 1 cut(s) 2209
XceI RCATGY 2 cut(s) 274, 1906
XhoI CTCGAG 1 cut(s) 2100
XmnI GAANNNNTTC 2 cut(s) 856, 2289
ZraI GACGTC 1 cut(s) 632
ZrmI AGTACT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.