Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008001

Physical Location & Seq

Genomic Coordinates Forward (+)

9379281 .. 9382725

Gene Structure 3445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1121600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGATTCCTTTAGGGGAAGATGGGACAGATAAAGTTGCTTCTGATATAGTACAGAAGAAAGAGAGTTCTGATAGTGAAATTCATGTGCCACATCAAGCTCCTAATAAGGGGATCTGTCCGTTGCAATCAGATCATAAAGGGACTGCTCAATTTCTAACACCTGAGAAAGCGTCACAGGTGCCTGATGGAGTTGTTACTGCGTCACAACCAAATCAAGAAGGAAGTGCTTCCTCTTTAACATCTGAGAAAACCCCACAAACTCCTGAAACCGCTGCACTTGCCTTGCAATCTGCTCAAGAAGGAACCACTCCATCTACTACACTTAAGAGAGTGTTAGACGATGGTTATCACTGGAGAAAATACGGCCAGAAATTTGTTAAGGGAAATTCATATGTAAGAAGTTACTACAGATGTACGCATCCAAAATGTGAAGTGAAGAAGCAAGTGGAACGTGCACTTAGTGGCCAGATAACAGATACTGTATACTTTGGTGAGCATGAACATCCTAAACTCCGAAGTGTCCCAGTAGCTGTTAGTTTTGTTGTGTCCATTGTTGAGGACGAAAGACCAAAAGTGCTTTTGTTAACTGATGTTGAAGACAAATCGTCAAATGCGCATGAGCACGAATCAAACCAGATTGAGCCAGTAGATCCTCCACAGCTTTCAAGCGTTGCAGTTACTGAAGCCGTGCAGGGTGTGCTCTCTCAGTCAAATAGACCGAGAGATGGCGATCCTGACCCTATAAGACAGAAGAAGGCAAAACATAATGGCAACTCATTACTGGTGGATAAGCCAGCTGTTGAACCACGTATGGTTGTTCAGACTATGAGTGAGGTTGATATAGTGAGTGATGGGTACAGATGGCGCAAATACGGGCAGAAATTAGTAAAAGGCAATCCAAATCCAAGGAGTTACTACAGATGCTCAAATCCTGGATGCCCTGTTAAGAAACATGTAGAGAGGGCGTCTCATGATTCAAAAGTCGTTATAGCCACATATGAGGGGCAACATGATCATGATATGCCCCCGACAAGGACTGTGACCCATAATACAGCAGCATCAAACGTGTTTACGACAGCCCGCATTTGTGAGTCTGGCACTATACCCGAAGGGAATGCTGTCTGCCGCGATACTAGCCCAGAACATGAAGTTAAACTAAGCAAGCAACGGAACGATGAGTCAAAAACTAAACCAAGTGATGTTGCTGGCTCAGATATGGTCGTTGATTCTGATCTAGGCTCCGAAAGAACACTACATGATCAATCGGTTGCCGAAGCATGTACGACAACGGAAAGCGACCCCCCTTACATGATTGTTTGCAGAGCAAATCATAAGAAAACTATTGAGATGGGAATTAAGTCAGAAGGCGTTGATATGGTGGCTTGCGACAATCTGCGTCCGGAAAGTAATCCACCGGAGCAAGAAAAGCCGAAAGCAGAACCTGTTGACAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0008270 GO:0009719 GO:0009725 GO:0009751 GO:0009863 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043565 GO:0045893 GO:0045935 GO:0046677 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

485

Amino Acids

53.15

Weight (kDa)

5.93

Isoelectric Point (pI)

45.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	113 - 169	9.7e-24	WRKY DNA -binding domain
WRKY	PF03106	284 - 340	2.4e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04880 AT2G04880
fragaria_vesca	FvH4_6g41210
malus_domestica	MD09G1121600.v1.1 MD17G1112600.v1.1
prunus_persica	Prupe.3G202000_v2.0.a1 Prupe.3G202000_v2.0.a1 Prupe.3G202000_v2.0.a1 Prupe.3G202000_v2.0.a1 Prupe.3G202000_v2.0.a1 Prupe.3G202000_v2.0.a1
pyrus_communis	pycom09g04600 pycom17g10430
rosa_chinensis	RchiOBHm_Chr2g0156771
rosa_laevigata	RLG00000020940
rosa_multiflora	Rmu_sc0008149.1_g000011
rosa_roxburghii	Rroxscaffold_2G00092740
rosa_rugosa	Rorug02G0456100 Rorug02G0456100
rosa_samantha	Rh2AG521500 Rh2BG535000 Rh2CG506400 Rh2DG544000
rosa_wichuraiana	Rw2G043060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	615
AccB1I	GGYRCC	1 cut(s)	178
AccI	GTMKAC	1 cut(s)	483
AccII	CGCG	1 cut(s)	1128
AccIII	TCCGGA	1 cut(s)	1399
AciI	CCGC	3 cut(s)	270, 1081, 1126
AclWI	GGATC	3 cut(s)	119, 644, 725
AcoI	YGGCCR	2 cut(s)	364, 463
AcsI	RAATTY	3 cut(s)	78, 371, 385
AcuI	CTGAAG	1 cut(s)	702
AcyI	GRCGYC	1 cut(s)	965
AdeI	CACNNNGTG	1 cut(s)	461
AfaI	GTAC	4 cut(s)	51, 415, 857, 1282
AfiI	CCNNNNNNNGG	3 cut(s)	107, 725, 1032
AflII	CTTAAG	1 cut(s)	323
AflIII	ACRYGT	2 cut(s)	952, 1065
AgsI	TTSAA	4 cut(s)	596, 666, 803, 978
AhdI	GACNNNNNGTC	1 cut(s)	604
AjnI	CCWGG	1 cut(s)	931
AluBI	AGCT	4 cut(s)	98, 530, 661, 797
AluI	AGCT	4 cut(s)	98, 530, 661, 797
Alw21I	GWGCWC	3 cut(s)	457, 624, 702
Alw26I	GTCTC	1 cut(s)	972
Alw44I	GTGCAC	1 cut(s)	453
AlwI	GGATC	3 cut(s)	119, 644, 725
AlwNI	CAGNNNCTG	4 cut(s)	479, 530, 680, 1442
Aor13HI	TCCGGA	1 cut(s)	1399
AoxI	GGCC	2 cut(s)	364, 463
ApaLI	GTGCAC	1 cut(s)	453
ApeKI	GCWGC	2 cut(s)	272, 1055
ApoI	RAATTY	3 cut(s)	78, 371, 385
Asp700I	GAANNNNTTC	1 cut(s)	226
AspLEI	GCGC	2 cut(s)	616, 867
AsuHPI	GGTGA	1 cut(s)	503
BaeGI	GKGCMC	1 cut(s)	457
BalI	TGGCCA	1 cut(s)	465
BanI	GGYRCC	1 cut(s)	178
BbsI	GAAGAC	1 cut(s)	603
Bbv12I	GWGCWC	3 cut(s)	457, 624, 702
BbvI	GCAGC	2 cut(s)	259, 1067
BccI	CCATC	8 cut(s)	14, 179, 319, 335, 719, 845, 855, 1342
BceAI	ACGGC	2 cut(s)	379, 671
BcgI	CGANNNNNNTGC	2 cut(s)	1097, 1131
BciT130I	CCWGG	1 cut(s)	933
BclI	TGATCA	2 cut(s)	1012, 1258
BcoDI	GTCTC	1 cut(s)	972
BfaI	CTAG	2 cut(s)	1134, 1235
BfmI	CTRYAG	2 cut(s)	406, 916
BfrI	CTTAAG	1 cut(s)	323
BisI	GCNGC	3 cut(s)	273, 1056, 1126
BlsI	GCNGC	3 cut(s)	274, 1057, 1127
Bme1390I	CCNGG	1 cut(s)	933
BmeRI	GACNNNNNGTC	1 cut(s)	604
BmiI	GGNNCC	3 cut(s)	180, 304, 1240
BmrFI	CCNGG	1 cut(s)	933
BmrI	ACTGGG	1 cut(s)	518
BmsI	GCATC	4 cut(s)	427, 911, 926, 1067
BmuI	ACTGGG	1 cut(s)	518
BpiI	GAAGAC	1 cut(s)	603
BplI	GAGNNNNNCTC	2 cut(s)	952, 984
BpmI	CTGGAG	1 cut(s)	373
BpuEI	CTTGAG	1 cut(s)	279
BsaAI	YACGTR	1 cut(s)	809
BsaBI	GATNNNNATC	3 cut(s)	345, 729, 1230
BsaHI	GRCGYC	1 cut(s)	965
BsaJI	CCNNGG	1 cut(s)	905
BsaWI	WCCGGW	2 cut(s)	1399, 1414
Bsc4I	CCNNNNNNNGG	3 cut(s)	107, 725, 1032
Bse1I	ACTGG	4 cut(s)	356, 524, 644, 786
Bse8I	GATNNNNATC	3 cut(s)	345, 729, 1230
BseAI	TCCGGA	1 cut(s)	1399
BseBI	CCWGG	1 cut(s)	933
BseDI	CCNNGG	1 cut(s)	905
BseGI	GGATG	3 cut(s)	418, 502, 941
BseJI	GATNNNNATC	3 cut(s)	345, 729, 1230
BseLI	CCNNNNNNNGG	3 cut(s)	107, 725, 1032
BseMII	CTCAG	4 cut(s)	153, 234, 719, 1224
BseNI	ACTGG	4 cut(s)	356, 524, 644, 786
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	2 cut(s)	259, 1067
BsgI	GTGCAG	2 cut(s)	258, 710
Bsh1236I	CGCG	1 cut(s)	1128
BshFI	GGCC	2 cut(s)	366, 465
BshNI	GGYRCC	1 cut(s)	178
BsiHKAI	GWGCWC	3 cut(s)	457, 624, 702
BsiSI	CCGG	2 cut(s)	1400, 1415
BslFI	GGGAC	3 cut(s)	37, 154, 506
BslI	CCNNNNNNNGG	3 cut(s)	107, 725, 1032
BsmAI	GTCTC	1 cut(s)	972
BsmBI	CGTCTC	1 cut(s)	972
BsmFI	GGGAC	3 cut(s)	37, 154, 506
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	2 cut(s)	366, 465
Bsp1286I	GDGCHC	3 cut(s)	457, 624, 702
Bsp13I	TCCGGA	1 cut(s)	1399
Bsp143I	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
BspACI	CCGC	3 cut(s)	270, 1081, 1126
BspANI	GGCC	2 cut(s)	366, 465
BspCNI	CTCAG	4 cut(s)	154, 235, 718, 1223
BspEI	TCCGGA	1 cut(s)	1399
BspFNI	CGCG	1 cut(s)	1128
BspHI	TCATGA	2 cut(s)	970, 1015
BspLI	GGNNCC	3 cut(s)	180, 304, 1240
BspPI	GGATC	3 cut(s)	119, 644, 725
BspT107I	GGYRCC	1 cut(s)	178
BspTI	CTTAAG	1 cut(s)	323
BsrI	ACTGG	4 cut(s)	356, 524, 644, 786
BssECI	CCNNGG	1 cut(s)	905
BssMI	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
BssNAI	GTATAC	1 cut(s)	484
BssNI	GRCGYC	1 cut(s)	965
BssT1I	CCWWGG	1 cut(s)	905
Bst1107I	GTATAC	1 cut(s)	484
Bst2UI	CCWGG	1 cut(s)	933
Bst4CI	ACNGT	3 cut(s)	481, 1039, 1451
BstACI	GRCGYC	1 cut(s)	965
BstAFI	CTTAAG	1 cut(s)	323
BstAPI	GCANNNNNTGC	1 cut(s)	697
BstBAI	YACGTR	1 cut(s)	809
BstC8I	GCNNGC	5 cut(s)	795, 1081, 1163, 1207, 1384
BstDEI	CTNAG	6 cut(s)	162, 243, 458, 705, 1157, 1210
BstF5I	GGATG	3 cut(s)	418, 502, 941
BstFNI	CGCG	1 cut(s)	1128
BstHHI	GCGC	2 cut(s)	616, 867
BstKTI	GATC	7 cut(s)	114, 133, 652, 733, 1015, 1234, 1261
BstMAI	GTCTC	1 cut(s)	972
BstMBI	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
BstMWI	GCNNNNNNNGC	4 cut(s)	278, 697, 1134, 1426
BstNI	CCWGG	1 cut(s)	933
BstNSI	RCATGY	2 cut(s)	956, 1281
BstSCI	CCNGG	1 cut(s)	931
BstSFI	CTRYAG	2 cut(s)	406, 916
BstSLI	GKGCMC	1 cut(s)	457
BstUI	CGCG	1 cut(s)	1128
BstV1I	GCAGC	2 cut(s)	259, 1067
BstV2I	GAAGAC	1 cut(s)	603
BstX2I	RGATCY	2 cut(s)	111, 649
BstYI	RGATCY	2 cut(s)	111, 649
BstZ17I	GTATAC	1 cut(s)	484
BsuRI	GGCC	2 cut(s)	366, 465
BtsCI	GGATG	3 cut(s)	418, 502, 941
BtsIMutI	CAGTG	1 cut(s)	349
Cac8I	GCNNGC	5 cut(s)	795, 1081, 1163, 1207, 1384
CaiI	CAGNNNCTG	4 cut(s)	479, 530, 680, 1442
CciI	TCATGA	2 cut(s)	970, 1015
CfoI	GCGC	2 cut(s)	616, 867
CseI	GACGC	4 cut(s)	159, 189, 954, 1385
Csp6I	GTAC	4 cut(s)	50, 414, 856, 1281
CviQI	GTAC	4 cut(s)	50, 414, 856, 1281
DdeI	CTNAG	6 cut(s)	162, 243, 458, 705, 1157, 1210
DpnI	GATC	7 cut(s)	113, 132, 651, 732, 1014, 1233, 1260
DpnII	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
DraIII	CACNNNGTG	1 cut(s)	461
DriI	GACNNNNNGTC	1 cut(s)	604
EaeI	YGGCCR	2 cut(s)	364, 463
Eam1105I	GACNNNNNGTC	1 cut(s)	604
Eco130I	CCWWGG	1 cut(s)	905
Eco57I	CTGAAG	1 cut(s)	702
EcoRII	CCWGG	1 cut(s)	931
EcoT14I	CCWWGG	1 cut(s)	905
ErhI	CCWWGG	1 cut(s)	905
Esp3I	CGTCTC	1 cut(s)	972
FaqI	GGGAC	3 cut(s)	37, 154, 506
FauI	CCCGC	1 cut(s)	1088
FauNDI	CATATG	2 cut(s)	391, 997
FbaI	TGATCA	2 cut(s)	1012, 1258
FblI	GTMKAC	1 cut(s)	483
Fnu4HI	GCNGC	3 cut(s)	273, 1056, 1126
FokI	GGATG	3 cut(s)	405, 489, 948
Fsp4HI	GCNGC	3 cut(s)	273, 1056, 1126
FspAI	RTGCGCAY	1 cut(s)	615
FspBI	CTAG	2 cut(s)	1134, 1235
FspI	TGCGCA	1 cut(s)	615
GlaI	GCGC	2 cut(s)	615, 866
GluI	GCNGC	3 cut(s)	273, 1056, 1126
GsuI	CTGGAG	1 cut(s)	373
HaeIII	GGCC	2 cut(s)	366, 465
HapII	CCGG	2 cut(s)	1400, 1415
HgaI	GACGC	4 cut(s)	159, 189, 954, 1385
HhaI	GCGC	2 cut(s)	616, 867
Hin1I	GRCGYC	1 cut(s)	965
Hin6I	GCGC	2 cut(s)	614, 865
HinP1I	GCGC	2 cut(s)	614, 865
HincII	GTYRAC	2 cut(s)	585, 1447
HindII	GTYRAC	2 cut(s)	585, 1447
HinfI	GANTC	6 cut(s)	4, 626, 974, 1091, 1178, 1226
HpaI	GTTAAC	1 cut(s)	585
HpaII	CCGG	2 cut(s)	1400, 1415
HphI	GGTGA	1 cut(s)	503
Hpy166II	GTNNAC	5 cut(s)	455, 484, 585, 1071, 1447
Hpy188III	TCNNGA	7 cut(s)	215, 263, 296, 734, 971, 1016, 1400
Hpy8I	GTNNAC	5 cut(s)	455, 484, 585, 1071, 1447
HpyAV	CCTTC	5 cut(s)	212, 293, 748, 1103, 1358
HpyCH4III	ACNGT	3 cut(s)	481, 1039, 1451
HpyCH4IV	ACGT	3 cut(s)	451, 808, 1065
HpyCH4V	TGCA	7 cut(s)	124, 275, 286, 455, 674, 691, 1320
HpyF10VI	GCNNNNNNNGC	4 cut(s)	278, 697, 1134, 1426
HpyF3I	CTNAG	6 cut(s)	162, 243, 458, 705, 1157, 1210
HpySE526I	ACGT	3 cut(s)	451, 808, 1065
Hsp92I	GRCGYC	1 cut(s)	965
HspAI	GCGC	2 cut(s)	614, 865
Kpn2I	TCCGGA	1 cut(s)	1399
Ksp22I	TGATCA	2 cut(s)	1012, 1258
KspAI	GTTAAC	1 cut(s)	585
Kzo9I	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
LmnI	GCTCC	3 cut(s)	103, 1244, 1417
Lsp1109I	GCAGC	2 cut(s)	259, 1067
LweI	GCATC	4 cut(s)	427, 911, 926, 1067
MaeI	CTAG	2 cut(s)	1134, 1235
MaeII	ACGT	3 cut(s)	451, 808, 1065
MaeIII	GTNAC	7 cut(s)	171, 193, 201, 401, 676, 911, 1039
MalI	GATC	7 cut(s)	113, 132, 651, 732, 1014, 1233, 1260
MboI	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
MboII	GAAGA	5 cut(s)	29, 67, 448, 608, 763
MflI	RGATCY	2 cut(s)	111, 649
MhlI	GDGCHC	3 cut(s)	457, 624, 702
MlsI	TGGCCA	1 cut(s)	465
MluCI	AATT	6 cut(s)	78, 149, 371, 385, 881, 1353
MluNI	TGGCCA	1 cut(s)	465
MlyI	GAGTC	2 cut(s)	1100, 1187
MnlI	CCTC	6 cut(s)	241, 550, 663, 826, 954, 994
Mox20I	TGGCCA	1 cut(s)	465
MroI	TCCGGA	1 cut(s)	1399
MroXI	GAANNNNTTC	1 cut(s)	226
MscI	TGGCCA	1 cut(s)	465
MseI	TTAA	8 cut(s)	236, 324, 378, 584, 945, 1152, 1356, 1453
MslI	CAYNNNNRTG	1 cut(s)	1014
Msp20I	TGGCCA	1 cut(s)	465
MspA1I	CMGCKG	2 cut(s)	272, 797
MspCI	CTTAAG	1 cut(s)	323
MspI	CCGG	2 cut(s)	1400, 1415
MspR9I	CCNGG	1 cut(s)	933
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	1 cut(s)	933
MvnI	CGCG	1 cut(s)	1128
MwoI	GCNNNNNNNGC	4 cut(s)	278, 697, 1134, 1426
NdeI	CATATG	2 cut(s)	391, 997
NdeII	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
NlaIV	GGNNCC	3 cut(s)	180, 304, 1240
NmuCI	GTSAC	3 cut(s)	171, 201, 1039
NsbI	TGCGCA	1 cut(s)	615
NspI	RCATGY	2 cut(s)	956, 1281
PagI	TCATGA	2 cut(s)	970, 1015
PciI	ACATGT	1 cut(s)	952
PctI	GAATGC	1 cut(s)	1120
PdmI	GAANNNNTTC	1 cut(s)	226
PfeI	GAWTC	4 cut(s)	4, 626, 974, 1226
PfoI	TCCNGGA	1 cut(s)	931
PkrI	GCNGC	3 cut(s)	274, 1057, 1127
PleI	GAGTC	2 cut(s)	1099, 1186
PpsI	GAGTC	2 cut(s)	1099, 1186
Ppu21I	YACGTR	1 cut(s)	809
PscI	ACATGT	1 cut(s)	952
Psp6I	CCWGG	1 cut(s)	931
PspGI	CCWGG	1 cut(s)	931
PspN4I	GGNNCC	3 cut(s)	180, 304, 1240
PstNI	CAGNNNCTG	4 cut(s)	479, 530, 680, 1442
PsuI	RGATCY	2 cut(s)	111, 649
PvuII	CAGCTG	1 cut(s)	797
RsaI	GTAC	4 cut(s)	51, 415, 857, 1282
RsaNI	GTAC	4 cut(s)	50, 414, 856, 1281
RseI	CAYNNNNRTG	1 cut(s)	1014
SaqAI	TTAA	8 cut(s)	236, 324, 378, 584, 945, 1152, 1356, 1453
SatI	GCNGC	3 cut(s)	273, 1056, 1126
Sau3AI	GATC	7 cut(s)	111, 130, 649, 730, 1012, 1231, 1258
SchI	GAGTC	2 cut(s)	1100, 1187
ScrFI	CCNGG	1 cut(s)	933
SduI	GDGCHC	3 cut(s)	457, 624, 702
SfaNI	GCATC	4 cut(s)	427, 911, 926, 1067
SfcI	CTRYAG	2 cut(s)	406, 916
SmiMI	CAYNNNNRTG	1 cut(s)	1014
SmlI	CTYRAG	2 cut(s)	294, 323
SmoI	CTYRAG	2 cut(s)	294, 323
Sse9I	AATT	6 cut(s)	78, 149, 371, 385, 881, 1353
SsiI	CCGC	3 cut(s)	270, 1081, 1126
SspMI	CTAG	2 cut(s)	1134, 1235
StyD4I	CCNGG	1 cut(s)	931
StyI	CCWWGG	1 cut(s)	905
TaaI	ACNGT	3 cut(s)	481, 1039, 1451
TaiI	ACGT	3 cut(s)	454, 811, 1068
TaqII	GACCGA	1 cut(s)	733
TasI	AATT	6 cut(s)	78, 149, 371, 385, 881, 1353
TatI	WGTACW	1 cut(s)	49
TauI	GCSGC	1 cut(s)	1128
TfiI	GAWTC	4 cut(s)	4, 626, 974, 1226
Tru1I	TTAA	8 cut(s)	236, 324, 378, 584, 945, 1152, 1356, 1453
Tru9I	TTAA	8 cut(s)	236, 324, 378, 584, 945, 1152, 1356, 1453
TscAI	CASTG	1 cut(s)	356
TseFI	GTSAC	3 cut(s)	171, 201, 1039
TseI	GCWGC	2 cut(s)	272, 1055
Tsp45I	GTSAC	3 cut(s)	171, 201, 1039
TspDTI	ATGAA	4 cut(s)	71, 378, 513, 1161
TspGWI	ACGGA	3 cut(s)	108, 1183, 1304
TspRI	CASTG	1 cut(s)	356
Vha464I	CTTAAG	1 cut(s)	323
VneI	GTGCAC	1 cut(s)	453
XapI	RAATTY	3 cut(s)	78, 371, 385
XceI	RCATGY	2 cut(s)	956, 1281
XmiI	GTMKAC	1 cut(s)	483
XmnI	GAANNNNTTC	1 cut(s)	226
XspI	CTAG	2 cut(s)	1134, 1235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.