MD09G1151000.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008876
Physical Location & Seq
Forward (+)
11925815 .. 11932171
6357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1151000.v1.1.491

Sequence Viewer

Length: 2478 bp
ATGGGGAGCGAGACGAAGAATGGAGTGGCGGCAGCGGCGGCGCAGTTTCCGCCGATGACGAATAAGATTATACAGAGCCTGAAGGAGATTGTTAACTGTCCGGAGCCTGAGATCTACTCGGTGCTTAAAGAGTGCAATATGGACCCGAACGACGCCGTTCAGCGCCTGCTCTCTTTAGATACTTTTCATGAAGTCAAGAGCAAACGTGAAAGAAGGAAAGAGATAAAGGAGACACAAGACTCCAAGCCACGAGTTCACAGCGCAGGTTCAAATCGTGGTGCTAAAGGTAGTAATGAGCATACTGGTGGATGGTGTGGATCAACTCAAACTGGTTCCAATGAGCCCGGTAAAGCTGCTTATAAGGGACAAAATGGGTTTGTTTCACCTCATACTCCAGCTTCTCACTTTACAGGAGGAGCCACAAGCCAGCAACCTTCATCTCAGCGTGAATCTTTTAGTACTTCAATAAGGCCAGGTGATGCAGTCTGTGTATCTCTACAACCTTCTACGGGAAATCAATCTACTTCCGGAACCAGCTTGGGGAATCTTTCAATGGCCGACATTGTTAAGATGGGTAGACCACCTAGCATGGGCTCACGTATTTTATCTGATACATCTTTGCATCAGGATGCATTTTCTACAAATTCATGTAATAGCCACGTTGAATCTTCCCAAACAGAGATGCACCAATATATGCACTTTCATAATCCTCGTGTGTCAGAGATGATACATAAAACTGGTGATAGTGCTGGACAGAACGATTTCCATGATGAATGGCCTGTGATTGAGCAGCCAACGGCTGTAAATAGGTCATCAGCTTTAAATGCAAATCAATCTAAGTTTTATATCAATGAATCCAATGTGCCAAGGGATTACCAGTCACATAAGGTCCAACTATCAGAGGGGAATATTAGTAGCGAAAGTCTTAGTTCAGACTGCATTGCTTTTGCTTCCAGTAGACAGAAGATGGTGGATGGTTCCGGTGGAAAATCATATTATGTTGATGATTTGTCTTCCAATTCTAGTTCCGACGAATCTCGTAGGTCTGCATATGAGGATGGAAAAGGAACTGTTATTGATTCAAATGCACCATATCCAAATCATTCTGCCTCTAATGATGTTTCTGTGGCATCAGCTACTATGAGAATGCAACAACTGAATTTAAGAAAGGAGGAAGATCAAGATAATTGTGCAGTAGTCCTTCCAAATAATTTGCAAGAATTGGCTGCCGATTGTTCGCATTTGAGTTTTGGCACCTTTAGACCGTCTCAAGGTTCTGCATTATCCAGCAAGCCATCTAATTCCGTGAAAAATGACTTGGGAGGATCTTCTGCAGGAGAAGCAGGAGTTGATGTTTTCTCAGCTGGGCGCTTGGATACAAGAAATTCAGGGTACAATCATGACGAACTTCTTGGATACATGTATAATACTGGGAGAGCAAGCATAGGTGCTAAAAATTATGATTTTCCAGTACCTTCACAGCCAGATCTGATAAAACAGGATATCCTTGAAGCAACCCATGGACATAGAGACAGCTCTACCTCATCACTACGTAATTTTAATTCTGAGAACATTGAAAGAGCAACTTCTGGATTGCCCTTTGCAAAGGCAGATCCTGAATTCAGAAATATTCCTCTTCAAAACAATATGGCATATTCAGATTCTATACGAAGTGATCTATTGGAGTCATTCCAACAGTTTTTGGTGAAGCCATCTGTGCCTTCAAGATACAACAGTGTAGTTTCATCCGTAAAGAACCCAACTAGTTCCATGTCCGAGGCTCTGAGACCAGGCGCTGTACCGTTATCTGAGGTGTCTTCTGTACTTCCTCAGCGCCATGCAGCCCGTTCATATGCTCAACCTACCCATTCTTACGAACAGTTAGCAAACATTATTGGGTACCCTTCCATGCCTCAGGCTCAGAACTACTCGCCTATGCCATCAAATTTGCAGCAAGAATATCTAGGTGGTAGTGCATTCCATCAGTCAGGGGCTAGCATGGAATACAACTTCCCTCAGTATAGAAGTGGAGCCTCAGTAAGCAGATTGCCTACGTCTGCTGCTGCTGCTGGTCGTGGAAGTTTTGGGGCCTCTAATAACAATCACGGGAGTTTCTTGCATAATGCATCTGCTACTCCCGGCAGCATAGCTGACTATGATGATGTTTTCCGCCCTCAGTACAAGGATGGAAACCATTTAACCGCTCTTTATCAGAGTTCAAGAACATTGCCTTCCGTTCCAGACAGTGCATTTAACAACTTACTTGGGCAGAATCAACAGCATGCAGGATATCGACAAGGGCAGGCTGGACAGCTGCCGTCCCAGTTGCAGAGTTATCGAGGACACCCGGATTTCTATCATTCCCAAGTGGACGTAACACAAGAACTTCAGCGGCAACGCCTCAATGATTTAGGCTTAGGAGGTTTCCAAGACCTGTCACCTCAGCAGTTGCACCAGATTTGGCAGGGGACCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

826

Amino Acids

89.56

Weight (kDa)

6.11

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 20 - 74 1.1e-28 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 254
Acc65I GGTACC 1 cut(s) 1899
AccB1I GGYRCC 2 cut(s) 1253, 1899
AccBSI CCGCTC 1 cut(s) 2204
AccI GTMKAC 2 cut(s) 577, 958
AccIII TCCGGA 2 cut(s) 100, 527
AciI CCGC 7 cut(s) 29, 35, 38, 50, 2170, 2202, 2392
AclWI GGATC 3 cut(s) 325, 1333, 1607
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 5 cut(s) 643, 1159, 1384, 1619, 1945
AcuI CTGAAG 2 cut(s) 101, 2372
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 7 cut(s) 460, 1394, 1473, 1800, 1824, 1901, 2180
AfiI CCNNNNNNNGG 4 cut(s) 509, 540, 590, 1271
AflIII ACRYGT 1 cut(s) 1419
AhlI ACTAGT 1 cut(s) 1763
AjnI CCWGG 2 cut(s) 472, 1789
AjuI GAANNNNNNNTTGG 2 cut(s) 1301, 1333
AluBI AGCT 9 cut(s) 353, 398, 537, 818, 1136, 1364, 1536, 2150, 2314
AluI AGCT 9 cut(s) 353, 398, 537, 818, 1136, 1364, 1536, 2150, 2314
Alw26I GTCTC 5 cut(s) 5, 224, 1272, 1524, 1780
AlwI GGATC 3 cut(s) 325, 1333, 1607
AlwNI CAGNNNCTG 4 cut(s) 79, 166, 1616, 1796
Aor13HI TCCGGA 2 cut(s) 100, 527
AoxI GGCC 4 cut(s) 470, 555, 776, 2088
ApoI RAATTY 5 cut(s) 643, 1159, 1384, 1619, 1945
Asp700I GAANNNNTTC 1 cut(s) 761
Asp718I GGTACC 1 cut(s) 1899
AspLEI GCGC 6 cut(s) 43, 165, 263, 1371, 1796, 1836
AspS9I GGNCC 4 cut(s) 142, 889, 2088, 2469
AsuC2I CCSGG 3 cut(s) 345, 2139, 2348
AsuHPI GGTGA 5 cut(s) 375, 488, 752, 1717, 2430
AsuNHI GCTAGC 1 cut(s) 1994
AvaII GGWCC 3 cut(s) 142, 889, 2469
AxyI CCTNAGG 1 cut(s) 1914
BaeI ACNNNNGTAYC 2 cut(s) 1720, 1753
BanI GGYRCC 2 cut(s) 1253, 1899
BanII GRGCYC 2 cut(s) 345, 596
BauI CACGAG 2 cut(s) 249, 711
BbsI GAAGAC 2 cut(s) 1005, 1809
BbvCI CCTCAGC 2 cut(s) 1830, 2442
BceAI ACGGC 3 cut(s) 140, 813, 2302
BcgI CGANNNNNNTGC 2 cut(s) 2318, 2352
BciT130I CCWGG 2 cut(s) 474, 1791
BciVI GTATCC 2 cut(s) 1369, 1409
BcnI CCSGG 3 cut(s) 345, 2139, 2348
BcoDI GTCTC 5 cut(s) 5, 224, 1272, 1524, 1780
BcuI ACTAGT 1 cut(s) 1763
BfaI CTAG 6 cut(s) 585, 1023, 1764, 1964, 1995, 2476
BfmI CTRYAG 1 cut(s) 1332
BfoI RGCGCY 4 cut(s) 166, 1372, 1797, 1837
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 2 cut(s) 1369, 1409
BglII AGATCT 2 cut(s) 111, 1486
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 5 cut(s) 345, 474, 1791, 2139, 2348
Bme18I GGWCC 3 cut(s) 142, 889, 2469
BmgT120I GGNCC 4 cut(s) 142, 889, 2088, 2469
BmrFI CCNGG 5 cut(s) 345, 474, 1791, 2139, 2348
BmrI ACTGGG 2 cut(s) 1440, 2317
BmsI GCATC 6 cut(s) 469, 619, 631, 672, 1139, 2135
BmtI GCTAGC 1 cut(s) 1998
BmuI ACTGGG 2 cut(s) 1440, 2317
BpiI GAAGAC 2 cut(s) 1005, 1809
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 378
Bpu10I CCTNAGC 3 cut(s) 1830, 2416, 2442
BpuEI CTTGAG 1 cut(s) 1254
BpuMI CCSGG 3 cut(s) 345, 2139, 2348
BsaAI YACGTR 2 cut(s) 599, 1553
BsaBI GATNNNNATC 1 cut(s) 2355
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 1780
BsaJI CCNNGG 3 cut(s) 866, 1519, 1776
BsaWI WCCGGW 3 cut(s) 100, 527, 980
BsaXI ACNNNNNCTCC 4 cut(s) 1338, 1368, 2022, 2052
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 540, 590, 1271
Bse1I ACTGG 8 cut(s) 307, 334, 742, 877, 954, 1435, 1469, 2323
Bse21I CCTNAGG 1 cut(s) 1914
Bse3DI GCAATG 2 cut(s) 939, 2225
Bse8I GATNNNNATC 1 cut(s) 2355
BseAI TCCGGA 2 cut(s) 100, 527
BseBI CCWGG 2 cut(s) 474, 1791
BseDI CCNNGG 3 cut(s) 866, 1519, 1776
BseGI GGATG 6 cut(s) 314, 634, 979, 1063, 1745, 2191
BseJI GATNNNNATC 1 cut(s) 2355
BseLI CCNNNNNNNGG 4 cut(s) 509, 540, 590, 1271
BseMI GCAATG 2 cut(s) 939, 2225
BseNI ACTGG 8 cut(s) 307, 334, 742, 877, 954, 1435, 1469, 2323
BseRI GAGGAG 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 1364
BsgI GTGCAG 1 cut(s) 1212
BshFI GGCC 4 cut(s) 472, 557, 778, 2090
BshNI GGYRCC 2 cut(s) 1253, 1899
BsiSI CCGG 6 cut(s) 101, 345, 528, 981, 2139, 2348
BslFI GGGAC 2 cut(s) 378, 2305
BslI CCNNNNNNNGG 4 cut(s) 509, 540, 590, 1271
BsmAI GTCTC 5 cut(s) 5, 224, 1272, 1524, 1780
BsmBI CGTCTC 2 cut(s) 5, 1272
BsmFI GGGAC 2 cut(s) 378, 2305
BsmI GAATGC 2 cut(s) 1152, 1976
BsnI GGCC 4 cut(s) 472, 557, 778, 2090
Bso31I GGTCTC 1 cut(s) 1780
Bsp1286I GDGCHC 2 cut(s) 345, 596
Bsp13I TCCGGA 2 cut(s) 100, 527
Bsp143I GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
Bsp19I CCATGG 1 cut(s) 1519
BspACI CCGC 7 cut(s) 29, 35, 38, 50, 2170, 2202, 2392
BspANI GGCC 4 cut(s) 472, 557, 778, 2090
BspEI TCCGGA 2 cut(s) 100, 527
BspHI TCATGA 2 cut(s) 187, 1399
BspMAI CTGCAG 1 cut(s) 1336
BspMI ACCTGC 1 cut(s) 254
BspOI GCTAGC 1 cut(s) 1998
BspPI GGATC 3 cut(s) 325, 1333, 1607
BspT107I GGYRCC 2 cut(s) 1253, 1899
BspTNI GGTCTC 1 cut(s) 1780
BsrBI CCGCTC 1 cut(s) 2204
BsrDI GCAATG 2 cut(s) 939, 2225
BsrI ACTGG 8 cut(s) 307, 334, 742, 877, 954, 1435, 1469, 2323
BssECI CCNNGG 3 cut(s) 866, 1519, 1776
BssMI GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 2 cut(s) 249, 711
BssT1I CCWWGG 2 cut(s) 866, 1519
Bst2BI CACGAG 2 cut(s) 249, 711
Bst2UI CCWGG 2 cut(s) 474, 1791
Bst4CI ACNGT 8 cut(s) 98, 1072, 1266, 1698, 1736, 1803, 1881, 2246
Bst6I CTCTTC 1 cut(s) 1641
BstACI GRCGYC 1 cut(s) 153
BstBAI YACGTR 2 cut(s) 599, 1553
BstC8I GCNNGC 7 cut(s) 167, 428, 1292, 1441, 1996, 2283, 2304
BstDSI CCRYGG 1 cut(s) 1519
BstF5I GGATG 6 cut(s) 314, 634, 979, 1063, 1745, 2191
BstH2I RGCGCY 4 cut(s) 166, 1372, 1797, 1837
BstHHI GCGC 6 cut(s) 43, 165, 263, 1371, 1796, 1836
BstKTI GATC 7 cut(s) 114, 320, 1180, 1328, 1489, 1615, 1678
BstMAI GTCTC 5 cut(s) 5, 224, 1272, 1524, 1780
BstMBI GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
BstMWI GCNNNNNNNGC 7 cut(s) 35, 38, 49, 824, 1340, 1717, 2066
BstNI CCWGG 2 cut(s) 474, 1791
BstNSI RCATGY 2 cut(s) 1423, 2285
BstSCI CCNGG 5 cut(s) 343, 472, 1789, 2137, 2346
BstSFI CTRYAG 1 cut(s) 1332
BstSNI TACGTA 1 cut(s) 1553
BstV2I GAAGAC 2 cut(s) 1005, 1809
BstX2I RGATCY 4 cut(s) 111, 1325, 1486, 1612
BstYI RGATCY 4 cut(s) 111, 1325, 1486, 1612
Bsu36I CCTNAGG 1 cut(s) 1914
BsuI GTATCC 2 cut(s) 1369, 1409
BsuRI GGCC 4 cut(s) 472, 557, 778, 2090
BtgI CCRYGG 1 cut(s) 1519
BtsCI GGATG 6 cut(s) 314, 634, 979, 1063, 1745, 2191
BtsIMutI CAGTG 2 cut(s) 1741, 2251
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 7 cut(s) 167, 428, 1292, 1441, 1996, 2283, 2304
CaiI CAGNNNCTG 4 cut(s) 79, 166, 1616, 1796
CciI TCATGA 2 cut(s) 187, 1399
CfoI GCGC 6 cut(s) 43, 165, 263, 1371, 1796, 1836
Cfr13I GGNCC 4 cut(s) 142, 889, 2088, 2469
CseI GACGC 1 cut(s) 161
Csp6I GTAC 7 cut(s) 459, 1393, 1472, 1799, 1823, 1900, 2179
CviQI GTAC 7 cut(s) 459, 1393, 1472, 1799, 1823, 1900, 2179
DpnI GATC 7 cut(s) 113, 319, 1179, 1327, 1488, 1614, 1677
DpnII GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
DraI TTTAAA 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 1641
EarI CTCTTC 1 cut(s) 1641
EciI GGCGGA 2 cut(s) 39, 2159
Eco105I TACGTA 1 cut(s) 1553
Eco130I CCWWGG 2 cut(s) 866, 1519
Eco24I GRGCYC 2 cut(s) 345, 596
Eco31I GGTCTC 1 cut(s) 1780
Eco32I GATATC 2 cut(s) 1504, 2291
Eco47I GGWCC 3 cut(s) 142, 889, 2469
Eco57I CTGAAG 2 cut(s) 101, 2372
Eco81I CCTNAGG 1 cut(s) 1914
EcoO109I RGGNCCY 2 cut(s) 2088, 2469
EcoRI GAATTC 1 cut(s) 1619
EcoRII CCWGG 2 cut(s) 472, 1789
EcoRV GATATC 2 cut(s) 1504, 2291
EcoT14I CCWWGG 2 cut(s) 866, 1519
EcoT22I ATGCAT 2 cut(s) 634, 2128
EcoT38I GRGCYC 2 cut(s) 345, 596
ErhI CCWWGG 2 cut(s) 866, 1519
Esp3I CGTCTC 2 cut(s) 5, 1272
FalI AAGNNNNNCTT 2 cut(s) 1570, 1602
FaqI GGGAC 2 cut(s) 378, 2305
FauNDI CATATG 2 cut(s) 1051, 1852
FblI GTMKAC 2 cut(s) 577, 958
FokI GGATG 6 cut(s) 321, 641, 986, 1070, 1732, 2198
FriOI GRGCYC 2 cut(s) 345, 596
FspBI CTAG 6 cut(s) 585, 1023, 1764, 1964, 1995, 2476
GlaI GCGC 6 cut(s) 42, 164, 262, 1370, 1795, 1835
GsaI CCCAGC 1 cut(s) 1368
GsuI CTGGAG 1 cut(s) 378
HaeII RGCGCY 4 cut(s) 166, 1372, 1797, 1837
HaeIII GGCC 4 cut(s) 472, 557, 778, 2090
HapII CCGG 6 cut(s) 101, 345, 528, 981, 2139, 2348
HgaI GACGC 1 cut(s) 161
HhaI GCGC 6 cut(s) 43, 165, 263, 1371, 1796, 1836
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 6 cut(s) 41, 163, 261, 1369, 1794, 1834
HinP1I GCGC 6 cut(s) 41, 163, 261, 1369, 1794, 1834
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 6 cut(s) 101, 345, 528, 981, 2139, 2348
HphI GGTGA 5 cut(s) 375, 488, 752, 1717, 2430
Hpy166II GTNNAC 5 cut(s) 94, 256, 578, 959, 2371
Hpy8I GTNNAC 5 cut(s) 94, 256, 578, 959, 2371
Hpy99I CGWCG 2 cut(s) 155, 1034
HpyAV CCTTC 9 cut(s) 76, 207, 444, 513, 1211, 1485, 1731, 1914, 2241
HpyCH4III ACNGT 8 cut(s) 98, 1072, 1266, 1698, 1736, 1803, 1881, 2246
HpyCH4IV ACGT 6 cut(s) 205, 598, 660, 1552, 2054, 2373
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 38, 49, 824, 1340, 1717, 2066
HpySE526I ACGT 6 cut(s) 205, 598, 660, 1552, 2054, 2373
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 6 cut(s) 41, 163, 261, 1369, 1794, 1834
Kpn2I TCCGGA 2 cut(s) 100, 527
KpnI GGTACC 1 cut(s) 1903
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
LmnI GCTCC 4 cut(s) 6, 103, 416, 2030
LweI GCATC 6 cut(s) 469, 619, 631, 672, 1139, 2135
MaeI CTAG 6 cut(s) 585, 1023, 1764, 1964, 1995, 2476
MaeII ACGT 6 cut(s) 205, 598, 660, 1552, 2054, 2373
MaeIII GTNAC 3 cut(s) 879, 2374, 2436
MalI GATC 7 cut(s) 113, 319, 1179, 1327, 1488, 1614, 1677
MbiI CCGCTC 1 cut(s) 2204
MboI GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
MboII GAAGA 8 cut(s) 28, 660, 976, 1005, 1187, 1320, 1628, 1809
MflI RGATCY 4 cut(s) 111, 1325, 1486, 1612
MhlI GDGCHC 2 cut(s) 345, 596
MlyI GAGTC 2 cut(s) 233, 1694
MmeI TCCRAC 3 cut(s) 916, 1053, 1717
Mph1103I ATGCAT 2 cut(s) 634, 2128
MroI TCCGGA 2 cut(s) 100, 527
MroXI GAANNNNTTC 1 cut(s) 761
MseI TTAA 8 cut(s) 93, 126, 567, 821, 1163, 1560, 2198, 2253
MslI CAYNNNNRTG 2 cut(s) 303, 627
MspA1I CMGCKG 4 cut(s) 35, 1364, 2314, 2392
MspI CCGG 6 cut(s) 101, 345, 528, 981, 2139, 2348
MspR9I CCNGG 5 cut(s) 345, 474, 1791, 2139, 2348
Mva1269I GAATGC 2 cut(s) 1152, 1976
MvaI CCWGG 2 cut(s) 474, 1791
MwoI GCNNNNNNNGC 7 cut(s) 35, 38, 49, 824, 1340, 1717, 2066
NciI CCSGG 3 cut(s) 345, 2139, 2348
NcoI CCATGG 1 cut(s) 1519
NdeI CATATG 2 cut(s) 1051, 1852
NdeII GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
NheI GCTAGC 1 cut(s) 1994
NmuCI GTSAC 2 cut(s) 879, 2436
NsiI ATGCAT 2 cut(s) 634, 2128
NspI RCATGY 2 cut(s) 1423, 2285
PaeI GCATGC 1 cut(s) 2285
PagI TCATGA 2 cut(s) 187, 1399
PciI ACATGT 1 cut(s) 1419
PctI GAATGC 2 cut(s) 1152, 1976
PdmI GAANNNNTTC 1 cut(s) 761
PfeI GAWTC 8 cut(s) 449, 544, 665, 854, 1034, 1079, 1661, 2272
PleI GAGTC 2 cut(s) 233, 1693
PpsI GAGTC 2 cut(s) 233, 1693
Ppu21I YACGTR 2 cut(s) 599, 1553
PpuMI RGGWCCY 1 cut(s) 2469
PscI ACATGT 1 cut(s) 1419
PsiI TTATAA 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 2469
Psp6I CCWGG 2 cut(s) 472, 1789
PspFI CCCAGC 1 cut(s) 1364
PspGI CCWGG 2 cut(s) 472, 1789
PspPI GGNCC 4 cut(s) 142, 889, 2088, 2469
PspPPI RGGWCCY 1 cut(s) 2469
PstI CTGCAG 1 cut(s) 1336
PstNI CAGNNNCTG 4 cut(s) 79, 166, 1616, 1796
PsuI RGATCY 4 cut(s) 111, 1325, 1486, 1612
PvuII CAGCTG 2 cut(s) 1364, 2314
RsaI GTAC 7 cut(s) 460, 1394, 1473, 1800, 1824, 1901, 2180
RsaNI GTAC 7 cut(s) 459, 1393, 1472, 1799, 1823, 1900, 2179
RseI CAYNNNNRTG 2 cut(s) 303, 627
SaqAI TTAA 8 cut(s) 93, 126, 567, 821, 1163, 1560, 2198, 2253
Sau3AI GATC 7 cut(s) 111, 317, 1177, 1325, 1486, 1612, 1675
Sau96I GGNCC 4 cut(s) 142, 889, 2088, 2469
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 2 cut(s) 233, 1694
ScrFI CCNGG 5 cut(s) 345, 474, 1791, 2139, 2348
SduI GDGCHC 2 cut(s) 345, 596
SfaNI GCATC 6 cut(s) 469, 619, 631, 672, 1139, 2135
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 3 cut(s) 142, 889, 2469
SmiMI CAYNNNNRTG 2 cut(s) 303, 627
SmlI CTYRAG 1 cut(s) 1269
SmoI CTYRAG 1 cut(s) 1269
SnaBI TACGTA 1 cut(s) 1553
SpeI ACTAGT 1 cut(s) 1763
SphI GCATGC 1 cut(s) 2285
SsiI CCGC 7 cut(s) 29, 35, 38, 50, 2170, 2202, 2392
SspI AATATT 2 cut(s) 910, 1630
SspMI CTAG 6 cut(s) 585, 1023, 1764, 1964, 1995, 2476
StyD4I CCNGG 5 cut(s) 343, 472, 1789, 2137, 2346
StyI CCWWGG 2 cut(s) 866, 1519
TaaI ACNGT 8 cut(s) 98, 1072, 1266, 1698, 1736, 1803, 1881, 2246
TaiI ACGT 6 cut(s) 208, 601, 663, 1555, 2057, 2376
TaqI TCGA 2 cut(s) 2293, 2338
TatI WGTACW 3 cut(s) 458, 1822, 2178
TauI GCSGC 4 cut(s) 32, 38, 41, 2395
TfiI GAWTC 8 cut(s) 449, 544, 665, 854, 1034, 1079, 1661, 2272
Tru1I TTAA 8 cut(s) 93, 126, 567, 821, 1163, 1560, 2198, 2253
Tru9I TTAA 8 cut(s) 93, 126, 567, 821, 1163, 1560, 2198, 2253
TscAI CASTG 2 cut(s) 1741, 2251
TseFI GTSAC 2 cut(s) 879, 2436
Tsp45I GTSAC 2 cut(s) 879, 2436
TspDTI ATGAA 9 cut(s) 176, 204, 426, 636, 692, 786, 867, 1734, 1839
TspGWI ACGGA 3 cut(s) 1294, 1738, 2224
TspRI CASTG 2 cut(s) 1741, 2251
VpaK11BI GGWCC 3 cut(s) 142, 889, 2469
XapI RAATTY 5 cut(s) 643, 1159, 1384, 1619, 1945
XceI RCATGY 2 cut(s) 1423, 2285
XmiI GTMKAC 2 cut(s) 577, 958
XmnI GAANNNNTTC 1 cut(s) 761
XspI CTAG 6 cut(s) 585, 1023, 1764, 1964, 1995, 2476
ZrmI AGTACT 1 cut(s) 460
Zsp2I ATGCAT 2 cut(s) 634, 2128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.