MD17G1138600.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008876
Physical Location & Seq
Forward (+)
12443038 .. 12449531
6494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1138600.v1.1.491

Sequence Viewer

Length: 2463 bp
ATGGGGAGCGAGAAGAAGAATGGAGTGGGGGTAGCGGCGGCGCAGTTTCCGCCGACGACGAGGAAGATTATACAGAGCCTGAAGGAGATTGTTAACTGTCCGGAGCCTGAGATCTACTCGGTTCTTAAAGAGTGCAGTATGGACCCGAGCGACGCCGTTCAGCGCCTGCTCTCTTTAGATACTTTTCATGAAGTCAAGAGCAAACGTGAAAGAAGGAAAGAGATAAAGGAGACACAAGACACCAAGTCGCGAGTTCACAATGCAGGTTCAAATCGTGGTGCTAGAGGTATTAATGAGTGTACTGGTGGATGGTGTGGATCAACTCAAACTAGTTCCAATGAGCTCGGTCAAGCAGCATATAAGGGACAGAATGGGTTTGTTGCTCCTTCACCGCATACTTCAGCTTCTCACTTTACAGGAGGAACCACAAGCCAGCAACCTTCATCTCAGCGTGAATCTTTTAGTACTTCCATAAGGCCAGGTGATGCAATCTCTGCATCTCTACAACCTTCTTCGGGAAATCAATCTACTTATGGAACCGGCTTGGGGAATCTTTCAATGGCCGACATTGTTAAGATGGGTAGACAACCTAGCATGGGCTCACATATTTCATCTGATACATCTTTGCATCAGGATGCATTTTCAACAAATTCATGTAATAGCCACGTGGAATCTTCCCAAACAGAGATGCACTCTCATAATCCTCGTGTGTCAGAGATGTTACATAAAACTGGCGATAGTGCTGGACAGAATTATTTCCATGATGAATGGCGTGTGATTGAGCAGCCAACAGCTGCAAATAGGTCATCAGCTTTAAATGCAAATCAATCTAAGTTTTATATCAATGAATCCAACGTGCCAAGGGATTACCAGTCACATAAGGTCCAAGAATCAGAGGGTAATATTAGTAGTGAAAGTGTTAGCTCAGATTGCAATGCTTATGCTTTTGCTTCCAGTAGACAGAAGATGGCGGATGCTTCCGGTGGAAAATTATATTGTGTTGATGATTTGTCTTCCAATTCTAGTTCCTACGAATCTCATAGGTCTGCATATGAGAATGGAGAAGGAACTGATATTGGTTCAAATGCGTCATATCCAAATCATTCTGTCTCTAATGATGTTGCTGTGGCATCAGCTACTGTGAGAATGCAACAACTGAATCTGAGAAAGGATGAAGATCAAGATAATTGTGCAGTAGTCCTTCCATATAATTTGCAAGAATTGGCTGCCGATTGTTCGCATTTGAGTTTTGGCACCTTTAGACCGGCTCAAAGTTCTGCATTATCGAGCAAGCCATCTAATTCCTTGAAAAATGACTTGGGAGGATCTTCTGCAGGAATAGATGTTATCTCAGCCAGTCGCTTGGATACAAGAAATTCAGGGTATACTCACGATGAACTTCTTGGATCCTTATATGATACTAGAAGAGCAGCCATAGGTTATGATTTGCCTGTACCTTCACAGCGAGAACCGATAAAACAGGATATCCTTGAAGCAGCCCGTGGATATAGGGACATTACTACCTCATCTCTACATAATTTTAATTCTGAGAAGATTGAAAAAGCAACTTCTGGATTGCCTTTTGCAAAGGCAGATCCCGAATTCAGAAATGTACCTAAAAATGATATGGCATATTCAGATTCTGCACGAAGTGACCTATTGGAGTCATTCCAACAGTTTTTGGTGAAGCCACCTGTGCCTTCAAGATATAGCAGTGCAGTTTCTTCTGTGAACAACCCAACAATTTCCATGTCTGAAGCTCTGAGACCAGGCACCGTACCCTTATCTGAGGCATGTTCTGTACTTCCTCAGTGTCATACAGCCCGTTCATACGCTCAGCCTACCCTTTCTTACGAACAGTTAGCAAACGGTATGGTCTATAAACCTTCCATTCCTCAGGCTCAGAACTACTCGCCCGTGCCACCAAATTTGCAGCAAGAGTATGTAGGTGGTAGTGCATTCCATATGTCAGGGGCTAGCATGGAATACAACTTCCCACAGTATAGTGGAGCCTCAGTAAGCAGATTGCCTACATCTGCTGCTGCTGCTGTCACTGGTCATGGAAGGTTTGGGGCCTCTAATAACACTCACGGGAGTTTATTGCATAATGCATCTGCTACTCCCGGCAGCATACCCGACTATGATGATGTATTCCGCCCCCAGTATAAAGATGGAAACCATTTAGCTGCTCTTTATCAGAGTTCAAGAACATTGCCTTCCGTTCCAGACAGAGCATTTAATAACTTACATGGGCATAATCAACAGCATGCAGGATATCGACAAGGGCAGGCTGGACAGCTGCCCACCCAGTTGCAGAATTATCTGGGACATCCCGATTTCTATTTTTCCCAAAAGGACGTAACACAAGAACATCAGCGACAACGCCTCAATGATTTAGGCTTAGGAGGATTCCAAGACCTGTCACCTCAGCAGCTGCACCAGATTTGGCAGCGGACCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

821

Amino Acids

89.12

Weight (kDa)

6.24

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 17 - 74 4e-28 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 254
AccB1I GGYRCC 2 cut(s) 1253, 1772
AccI GTMKAC 3 cut(s) 583, 958, 1385
AccII CGCG 1 cut(s) 250
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 7 cut(s) 35, 38, 50, 392, 971, 2155, 2452
AclWI GGATC 5 cut(s) 325, 1333, 1401, 1414, 1589
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 4 cut(s) 649, 1375, 1601, 1927
AcuI CTGAAG 3 cut(s) 101, 384, 1776
AcvI CACGTG 1 cut(s) 667
AcyI GRCGYC 1 cut(s) 153
AdeI CACNNNGTG 1 cut(s) 1652
AfaI GTAC 6 cut(s) 301, 466, 1455, 1614, 1779, 1803
AfiI CCNNNNNNNGG 3 cut(s) 515, 546, 596
AgsI TTSAA 9 cut(s) 270, 558, 645, 1083, 1309, 1493, 1559, 1704, 2205
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 2 cut(s) 478, 1768
AjuI GAANNNNNNNTTGG 2 cut(s) 1301, 1333
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 3 cut(s) 224, 1114, 1759
AlwI GGATC 5 cut(s) 325, 1333, 1401, 1414, 1589
AlwNI CAGNNNCTG 5 cut(s) 79, 166, 1139, 1643, 2434
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 3 cut(s) 476, 561, 2073
ApoI RAATTY 4 cut(s) 649, 1375, 1601, 1927
AseI ATTAAT 1 cut(s) 291
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 2 cut(s) 43, 165
AspS9I GGNCC 4 cut(s) 142, 883, 2073, 2454
AsuC2I CCSGG 1 cut(s) 2124
AsuHPI GGTGA 4 cut(s) 381, 494, 1696, 2415
AsuNHI GCTAGC 1 cut(s) 1976
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 3 cut(s) 142, 883, 2454
AxyI CCTNAGG 1 cut(s) 1896
BamHI GGATCC 1 cut(s) 1406
BanI GGYRCC 2 cut(s) 1253, 1772
BanII GRGCYC 2 cut(s) 345, 602
BauI CACGAG 1 cut(s) 705
BbrPI CACGTG 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 2427
BccI CCATC 5 cut(s) 303, 571, 961, 1303, 2165
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 2 cut(s) 480, 1770
BciVI GTATCC 1 cut(s) 1360
BcnI CCSGG 1 cut(s) 2124
BcoDI GTCTC 3 cut(s) 224, 1114, 1759
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 6 cut(s) 282, 330, 591, 1023, 1422, 1977
BfmI CTRYAG 1 cut(s) 1332
BfoI RGCGCY 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 1 cut(s) 1360
BglII AGATCT 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 1836
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 3 cut(s) 480, 1770, 2124
Bme18I GGWCC 3 cut(s) 142, 883, 2454
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 142, 883, 2073, 2454
BmiI GGNNCC 9 cut(s) 105, 144, 424, 538, 1255, 1408, 1774, 2011, 2074
BmrFI CCNGG 3 cut(s) 480, 1770, 2124
BmrI ACTGGG 2 cut(s) 2155, 2302
BmsI GCATC 8 cut(s) 475, 506, 625, 637, 678, 964, 1139, 2120
BmtI GCTAGC 1 cut(s) 1980
BmuI ACTGGG 2 cut(s) 2155, 2302
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 4 cut(s) 101, 133, 677, 709
Bpu10I CCTNAGC 2 cut(s) 2401, 2427
Bpu1102I GCTNAGC 1 cut(s) 1836
BpuMI CCSGG 1 cut(s) 2124
BsaAI YACGTR 1 cut(s) 667
BsaBI GATNNNNATC 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 1759
BsaJI CCNNGG 2 cut(s) 860, 1501
BsaWI WCCGGW 2 cut(s) 100, 980
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 2001, 2031
Bsc4I CCNNNNNNNGG 3 cut(s) 515, 546, 596
Bse118I RCCGGY 2 cut(s) 539, 1264
Bse1I ACTGG 8 cut(s) 307, 736, 871, 954, 1356, 2059, 2161, 2308
Bse21I CCTNAGG 1 cut(s) 1896
Bse3DI GCAATG 2 cut(s) 940, 2210
Bse8I GATNNNNATC 1 cut(s) 1176
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 2 cut(s) 480, 1770
BseDI CCNNGG 2 cut(s) 860, 1501
BseGI GGATG 5 cut(s) 314, 640, 979, 1177, 2329
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 3 cut(s) 515, 546, 596
BseMI GCAATG 2 cut(s) 940, 2210
BseNI ACTGG 8 cut(s) 307, 736, 871, 954, 1356, 2059, 2161, 2308
BsgI GTGCAG 5 cut(s) 154, 1212, 1629, 1737, 2420
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 478, 563, 2075
BshNI GGYRCC 2 cut(s) 1253, 1772
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 5 cut(s) 101, 540, 981, 1265, 2124
BslFI GGGAC 3 cut(s) 378, 1526, 2340
BslI CCNNNNNNNGG 3 cut(s) 515, 546, 596
BsmAI GTCTC 3 cut(s) 224, 1114, 1759
BsmFI GGGAC 3 cut(s) 378, 1526, 2340
BsmI GAATGC 2 cut(s) 1152, 1958
BsnI GGCC 3 cut(s) 478, 563, 2075
Bso31I GGTCTC 1 cut(s) 1759
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 345, 602
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
Bsp1720I GCTNAGC 1 cut(s) 1836
Bsp68I TCGCGA 1 cut(s) 250
BspACI CCGC 7 cut(s) 35, 38, 50, 392, 971, 2155, 2452
BspANI GGCC 3 cut(s) 478, 563, 2075
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 250
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 9 cut(s) 105, 144, 424, 538, 1255, 1408, 1774, 2011, 2074
BspMAI CTGCAG 1 cut(s) 1336
BspMI ACCTGC 1 cut(s) 254
BspOI GCTAGC 1 cut(s) 1980
BspPI GGATC 5 cut(s) 325, 1333, 1401, 1414, 1589
BspQI GCTCTTC 1 cut(s) 1420
BspT107I GGYRCC 2 cut(s) 1253, 1772
BspTNI GGTCTC 1 cut(s) 1759
BsrDI GCAATG 2 cut(s) 940, 2210
BsrFI RCCGGY 2 cut(s) 539, 1264
BsrI ACTGG 8 cut(s) 307, 736, 871, 954, 1356, 2059, 2161, 2308
BssAI RCCGGY 2 cut(s) 539, 1264
BssECI CCNNGG 2 cut(s) 860, 1501
BssMI GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
BssNAI GTATAC 1 cut(s) 1386
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 860
Bst1107I GTATAC 1 cut(s) 1386
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 480, 1770
Bst4CI ACNGT 7 cut(s) 98, 1141, 1677, 1777, 1860, 1871, 2001
Bst6I CTCTTC 1 cut(s) 1420
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 6 cut(s) 167, 434, 1292, 1978, 2268, 2289
BstDSI CCRYGG 1 cut(s) 1501
BstF5I GGATG 5 cut(s) 314, 640, 979, 1177, 2329
BstFNI CGCG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 2 cut(s) 43, 165
BstKTI GATC 6 cut(s) 114, 320, 1180, 1328, 1409, 1597
BstMAI GTCTC 3 cut(s) 224, 1114, 1759
BstMBI GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
BstMWI GCNNNNNNNGC 6 cut(s) 49, 494, 818, 930, 1696, 2045
BstNI CCWGG 2 cut(s) 480, 1770
BstNSI RCATGY 2 cut(s) 1797, 2270
BstSCI CCNGG 3 cut(s) 478, 1768, 2122
BstSFI CTRYAG 1 cut(s) 1332
BstUI CGCG 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 4 cut(s) 111, 1325, 1406, 1594
BstXI CCANNNNNNTGG 1 cut(s) 1363
BstYI RGATCY 4 cut(s) 111, 1325, 1406, 1594
BstZ17I GTATAC 1 cut(s) 1386
Bsu36I CCTNAGG 1 cut(s) 1896
BsuI GTATCC 1 cut(s) 1360
BsuRI GGCC 3 cut(s) 478, 563, 2075
BtgI CCRYGG 1 cut(s) 1501
BtsCI GGATG 5 cut(s) 314, 640, 979, 1177, 2329
BtsI GCAGTG 1 cut(s) 1720
BtsIMutI CAGTG 3 cut(s) 1720, 1817, 2052
BtuMI TCGCGA 1 cut(s) 250
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 6 cut(s) 167, 434, 1292, 1978, 2268, 2289
CaiI CAGNNNCTG 5 cut(s) 79, 166, 1139, 1643, 2434
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 43, 165
Cfr10I RCCGGY 2 cut(s) 539, 1264
Cfr13I GGNCC 4 cut(s) 142, 883, 2073, 2454
CseI GACGC 2 cut(s) 161, 1077
Csp6I GTAC 6 cut(s) 300, 465, 1454, 1613, 1778, 1802
CspCI CAANNNNNGTGG 2 cut(s) 1911, 1946
CviQI GTAC 6 cut(s) 300, 465, 1454, 1613, 1778, 1802
DpnI GATC 6 cut(s) 113, 319, 1179, 1327, 1408, 1596
DpnII GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
DraI TTTAAA 1 cut(s) 816
DraIII CACNNNGTG 1 cut(s) 1652
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 1420
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 1420
EciI GGCGGA 3 cut(s) 39, 986, 2144
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 860
Eco24I GRGCYC 2 cut(s) 345, 602
Eco31I GGTCTC 1 cut(s) 1759
Eco32I GATATC 2 cut(s) 1486, 2276
Eco47I GGWCC 3 cut(s) 142, 883, 2454
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 3 cut(s) 101, 384, 1776
Eco72I CACGTG 1 cut(s) 667
Eco81I CCTNAGG 1 cut(s) 1896
Eco88I CYCGRG 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 2073
EcoRI GAATTC 1 cut(s) 1601
EcoRII CCWGG 2 cut(s) 478, 1768
EcoRV GATATC 2 cut(s) 1486, 2276
EcoT14I CCWWGG 1 cut(s) 860
EcoT22I ATGCAT 2 cut(s) 640, 2113
EcoT38I GRGCYC 2 cut(s) 345, 602
ErhI CCWWGG 1 cut(s) 860
FaqI GGGAC 3 cut(s) 378, 1526, 2340
FauNDI CATATG 2 cut(s) 1051, 1965
FblI GTMKAC 3 cut(s) 583, 958, 1385
FokI GGATG 5 cut(s) 321, 647, 986, 1184, 2316
FriOI GRGCYC 2 cut(s) 345, 602
FspBI CTAG 6 cut(s) 282, 330, 591, 1023, 1422, 1977
GlaI GCGC 2 cut(s) 42, 164
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 3 cut(s) 478, 563, 2075
HapII CCGG 5 cut(s) 101, 540, 981, 1265, 2124
HgaI GACGC 2 cut(s) 161, 1077
HhaI GCGC 2 cut(s) 43, 165
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 2 cut(s) 41, 163
HinP1I GCGC 2 cut(s) 41, 163
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 5 cut(s) 101, 540, 981, 1265, 2124
HphI GGTGA 4 cut(s) 381, 494, 1696, 2415
Hpy166II GTNNAC 7 cut(s) 94, 256, 300, 584, 959, 1386, 1732
Hpy8I GTNNAC 7 cut(s) 94, 256, 300, 584, 959, 1386, 1732
Hpy99I CGWCG 3 cut(s) 58, 61, 155
HpyCH4III ACNGT 7 cut(s) 98, 1141, 1677, 1777, 1860, 1871, 2001
HpyCH4IV ACGT 4 cut(s) 205, 666, 855, 2358
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 494, 818, 930, 1696, 2045
HpySE526I ACGT 4 cut(s) 205, 666, 855, 2358
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 2 cut(s) 41, 163
Kpn2I TCCGGA 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
LguI GCTCTTC 1 cut(s) 1420
LmnI GCTCC 4 cut(s) 6, 103, 388, 2009
LweI GCATC 8 cut(s) 475, 506, 625, 637, 678, 964, 1139, 2120
MaeI CTAG 6 cut(s) 282, 330, 591, 1023, 1422, 1977
MaeII ACGT 4 cut(s) 205, 666, 855, 2358
MaeIII GTNAC 6 cut(s) 720, 873, 1652, 2050, 2359, 2421
MalI GATC 6 cut(s) 113, 319, 1179, 1327, 1408, 1596
MboI GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
MflI RGATCY 4 cut(s) 111, 1325, 1406, 1594
MhlI GDGCHC 2 cut(s) 345, 602
MlyI GAGTC 1 cut(s) 1673
MmeI TCCRAC 2 cut(s) 876, 1696
Mph1103I ATGCAT 2 cut(s) 640, 2113
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 7 cut(s) 93, 126, 291, 573, 815, 1542, 2238
MslI CAYNNNNRTG 1 cut(s) 633
MspA1I CMGCKG 4 cut(s) 794, 2299, 2434, 2452
MspI CCGG 5 cut(s) 101, 540, 981, 1265, 2124
MspR9I CCNGG 3 cut(s) 480, 1770, 2124
Mva1269I GAATGC 2 cut(s) 1152, 1958
MvaI CCWGG 2 cut(s) 480, 1770
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 6 cut(s) 49, 494, 818, 930, 1696, 2045
NciI CCSGG 1 cut(s) 2124
NdeI CATATG 2 cut(s) 1051, 1965
NdeII GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
NheI GCTAGC 1 cut(s) 1976
NlaIV GGNNCC 9 cut(s) 105, 144, 424, 538, 1255, 1408, 1774, 2011, 2074
NmuCI GTSAC 4 cut(s) 873, 1652, 2050, 2421
NruI TCGCGA 1 cut(s) 250
NsiI ATGCAT 2 cut(s) 640, 2113
NspI RCATGY 2 cut(s) 1797, 2270
PaeI GCATGC 1 cut(s) 2270
PagI TCATGA 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 1420
PctI GAATGC 2 cut(s) 1152, 1958
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 9 cut(s) 455, 550, 671, 848, 890, 1034, 1159, 1640, 2409
PleI GAGTC 1 cut(s) 1672
PmaCI CACGTG 1 cut(s) 667
PmlI CACGTG 1 cut(s) 667
PpsI GAGTC 1 cut(s) 1672
Ppu21I YACGTR 1 cut(s) 667
PshBI ATTAAT 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 478, 1768
PspCI CACGTG 1 cut(s) 667
PspGI CCWGG 2 cut(s) 478, 1768
PspN4I GGNNCC 9 cut(s) 105, 144, 424, 538, 1255, 1408, 1774, 2011, 2074
PspPI GGNCC 4 cut(s) 142, 883, 2073, 2454
PstI CTGCAG 1 cut(s) 1336
PstNI CAGNNNCTG 5 cut(s) 79, 166, 1139, 1643, 2434
PsuI RGATCY 4 cut(s) 111, 1325, 1406, 1594
PvuII CAGCTG 3 cut(s) 794, 2299, 2434
RruI TCGCGA 1 cut(s) 250
RsaI GTAC 6 cut(s) 301, 466, 1455, 1614, 1779, 1803
RsaNI GTAC 6 cut(s) 300, 465, 1454, 1613, 1778, 1802
RseI CAYNNNNRTG 1 cut(s) 633
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 1420
SaqAI TTAA 7 cut(s) 93, 126, 291, 573, 815, 1542, 2238
Sau3AI GATC 6 cut(s) 111, 317, 1177, 1325, 1406, 1594
Sau96I GGNCC 4 cut(s) 142, 883, 2073, 2454
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 1673
ScrFI CCNGG 3 cut(s) 480, 1770, 2124
SduI GDGCHC 2 cut(s) 345, 602
SfaNI GCATC 8 cut(s) 475, 506, 625, 637, 678, 964, 1139, 2120
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 3 cut(s) 142, 883, 2454
SmiMI CAYNNNNRTG 1 cut(s) 633
SpeI ACTAGT 1 cut(s) 329
SphI GCATGC 1 cut(s) 2270
SsiI CCGC 7 cut(s) 35, 38, 50, 392, 971, 2155, 2452
SspI AATATT 1 cut(s) 904
SspMI CTAG 6 cut(s) 282, 330, 591, 1023, 1422, 1977
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 3 cut(s) 478, 1768, 2122
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 7 cut(s) 98, 1141, 1677, 1777, 1860, 1871, 2001
TaiI ACGT 4 cut(s) 208, 669, 858, 2361
TaqI TCGA 2 cut(s) 1286, 2278
TaqII GACCGA 1 cut(s) 335
TatI WGTACW 3 cut(s) 299, 464, 1801
TauI GCSGC 2 cut(s) 38, 41
TfiI GAWTC 9 cut(s) 455, 550, 671, 848, 890, 1034, 1159, 1640, 2409
Tru1I TTAA 7 cut(s) 93, 126, 291, 573, 815, 1542, 2238
Tru9I TTAA 7 cut(s) 93, 126, 291, 573, 815, 1542, 2238
TscAI CASTG 3 cut(s) 1720, 1817, 2059
TseFI GTSAC 4 cut(s) 873, 1652, 2050, 2421
Tsp45I GTSAC 4 cut(s) 873, 1652, 2050, 2421
TspGWI ACGGA 1 cut(s) 2209
TspRI CASTG 3 cut(s) 1720, 1817, 2059
VpaK11BI GGWCC 3 cut(s) 142, 883, 2454
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 4 cut(s) 649, 1375, 1601, 1927
XceI RCATGY 2 cut(s) 1797, 2270
XcmI CCANNNNNNNNNTGG 1 cut(s) 2168
XmiI GTMKAC 3 cut(s) 583, 958, 1385
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 6 cut(s) 282, 330, 591, 1023, 1422, 1977
ZrmI AGTACT 1 cut(s) 466
Zsp2I ATGCAT 2 cut(s) 640, 2113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.