MD09G1165200.v1.1

V-type proton ATPase catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008079
Physical Location & Seq
Reverse (-)
13521834 .. 13527397
5564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1165200.v1.1.491

Sequence Viewer

Length: 1872 bp
ATGCCGGCGGTGTACGGTGCCCGATTGACCACCTTTGAGGACTCCGAGAAGGAGAGTGAGTATGGCTACGTCCGCAAGGTCTCTGGACCAGTCGTGGTTGCTGATGGCATGGCTGGAGCTGCTATGTACGAGTTGGTTCGTGTTGGACATGATAATTTGATTGGTGAAATTATTCGGTTAGAAGGAGATTCTGCCACAATTCAAGTTTATGAGGAAACAGCTGGGTTGATGGTGAACGATCCGGTTCTACGCACTCGCAAGCCTTTATCAGTGGAGTTGGGACCTGGAATTTTGGGAAATATTTTTGACGGAATCCAGAGGCCCCTGAAAACTATTGCAAAGAGATCTGGTGATGTCTATATCCCACGTGGTGTATCTGTCCCAGCTCTTGACAAAGATATACTTTGGGAGTTTCAACCTAAAAAAATAGGTGAAGGTGATCATCTTACTGGTGGAGACTTGTACGCTACGGTCTTTGAAAATAGTCTGATGGAGCACCGAGTTGCACTTCCTCCCGATGCTATGGGAAAAGTTACTTACGTCGCACCACCTGGTCAATATTCATTGAAGGATACCGTGTTAGAGCTTGAGTTTCAAGGACAAAAAAAGAAATTTACAATGCTTCAGACTTGGCCTGTACGTACACCTAGGCCTGTTGCATCAAAGCTTGCTGCTGATACCCCTCTACTCACTGGGCAGCGTGTTCTTGATGCCCTTTTCCCCTCTGTTCTTGGTGGGACTTGTGCCATCCCTGGGGCTTTTGGTTGTGGAAAAACGGTTATTAGTCAAGCTCTTTCAAAGTACTCAAACTCAGACACTGTGGTTTATGTTGGTTGTGGAGAACGTGGAAATGAAATGGCAGAGGTGCTTATGGATTTCCCTCAATTGACAATGACTTTGCCTGATGGTCGTGAAGAATCTGTCATGAAGCGCACAACCCTTGTGGCCAACACTTCTAACATGCCTGTGGCCGCTCGTGAAGCTTCAATCTATACTGGAATCACTATTGCTGAATACTTCAGAGATATGGGTTACAATGTCAGCATGATGGCAGATTCAACATCTCGATGGGCTGAGGCATTGCGTGAGATTTCTGGGCGGCTGGCTGAAATGCCTGCAGATAGTGGATACCCTGCTTATTTGGCAGCACGTTTAGCATCATTTTATGAACGGGCTGGTAAAGTAAAATGTCTTGGCGGGCCAGAGCGTAATGGCAGTGTGACAATTGTTGGTGCTGTTTCTCCTCCAGGAGGAGATTTCTCAGACCCTGTGACATCTGCAACCCTTAGCATTGTTCAGGTTTTCTGGGGTCTGGACAAAAAACTTGCCCAGAGGAAGCATTTCCCCTCTGTGAACTGGCTTATTTCTTACTCGAAGTACTCAACGGCATTAGAGTCTTTCTATGATCAATTTGATCCTGATTTTATCAACATCAGGACAAAGGCACGTGAGGTTTTGCAACGAGAAGATGATCTCAATGAAATTGTCCAACTTGTAGGAAAGGATGCTTTGGCTGAAGGAGATAAGATCACCTTAGAGACTGCAAAGCTCTTGAGGGAGGATTATCTTGCGCAGAATGCATTTACTCCATATGACAAGTTCTGCCCATTCTACAAGTCCGTCTGGATGATGCGGAATATCATCCATTACTTTAATCTGGCCAATCAGGCTGTAGAGAGAGCGGCTGGTATGGATGGTCAGAAGATAAGTTACAGCCTAATCAAGCACCGTTTGGGAGATCTCTTTTACCGCCTAGTGTCCCAAAAGTTTGAGGATCCGGCAGAAGGGGAAGAAGCTCTTGTGGCAAAGTTTAAGAAGCTACACGAGGACCTCACAAGTGGTTTCCGTGCTCTTGAGGATGAAACCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0002020 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0006996 GO:0007030 GO:0007275 GO:0008150 GO:0008324 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009555 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010256 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019899 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0046961 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N ATP-synt_ab_Xtn

Protein Analysis

624

Amino Acids

68.83

Weight (kDa)

5.43

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 25 - 85 2.8e-15 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab_Xtn PF16886 127 - 214 9.9e-29 ATPsynthase alpha/beta subunit barrel-sandwich domain
ATP-synt_ab PF00006 232 - 458 9e-75 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 466 - 559 3.4e-28 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1572
AccB1I GGYRCC 1 cut(s) 17
AccBSI CCGCTC 2 cut(s) 974, 1682
AciI CCGC 8 cut(s) 8, 73, 972, 1099, 1197, 1633, 1682, 1750
AclWI GGATC 4 cut(s) 233, 1409, 1769, 1782
AcoI YGGCCR 3 cut(s) 945, 969, 1659
AcsI RAATTY 2 cut(s) 288, 611
AcuI CTGAAG 3 cut(s) 608, 1003, 1536
AcvI CACGTG 2 cut(s) 368, 1448
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 7 cut(s) 14, 128, 464, 639, 643, 803, 1379
AfiI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
AgsI TTSAA 8 cut(s) 203, 416, 479, 568, 596, 798, 987, 1059
AjnI CCWGG 4 cut(s) 283, 550, 751, 1246
Alw21I GWGCWC 2 cut(s) 498, 1852
Alw26I GTCTC 3 cut(s) 85, 450, 1532
AlwI GGATC 4 cut(s) 233, 1409, 1769, 1782
AlwNI CAGNNNCTG 2 cut(s) 818, 1268
AoxI GGCC 7 cut(s) 320, 632, 650, 945, 969, 1199, 1659
ApeKI GCWGC 4 cut(s) 119, 671, 697, 1145
ApoI RAATTY 2 cut(s) 288, 611
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
Asp700I GAANNNNTTC 2 cut(s) 171, 1340
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 2 cut(s) 933, 1573
AspS9I GGNCC 5 cut(s) 86, 281, 321, 1199, 1828
AsuHPI GGTGA 6 cut(s) 176, 244, 362, 443, 449, 1522
AvaII GGWCC 3 cut(s) 86, 281, 1828
AvrII CCTAGG 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 2 cut(s) 947, 1661
BamHI GGATCC 1 cut(s) 1774
BanI GGYRCC 1 cut(s) 17
BarI GAAGNNNNNNTAC 2 cut(s) 1694, 1726
BauI CACGAG 2 cut(s) 975, 1823
BbrPI CACGTG 2 cut(s) 368, 1448
Bbv12I GWGCWC 2 cut(s) 498, 1852
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 4 cut(s) 106, 658, 709, 1157
BccI CCATC 8 cut(s) 98, 223, 484, 755, 899, 1042, 1062, 1688
BceAI ACGGC 1 cut(s) 1401
BciT130I CCWGG 4 cut(s) 285, 552, 753, 1248
BciVI GTATCC 2 cut(s) 565, 1121
BclI TGATCA 2 cut(s) 439, 1405
BcoDI GTCTC 3 cut(s) 85, 450, 1532
BfaI CTAG 2 cut(s) 648, 1754
BfmI CTRYAG 2 cut(s) 1116, 1671
BfuI GTATCC 2 cut(s) 565, 1121
BglI GCCNNNNNGGC 1 cut(s) 1667
BglII AGATCT 2 cut(s) 344, 1738
BisI GCNGC 7 cut(s) 120, 672, 698, 972, 1100, 1146, 1683
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 7 cut(s) 121, 673, 699, 973, 1101, 1147, 1684
BmcAI AGTACT 2 cut(s) 803, 1379
Bme1390I CCNGG 4 cut(s) 285, 552, 753, 1248
Bme18I GGWCC 3 cut(s) 86, 281, 1828
BmgT120I GGNCC 5 cut(s) 86, 281, 321, 1199, 1828
BmiI GGNNCC 4 cut(s) 19, 282, 323, 1776
BmrFI CCNGG 4 cut(s) 285, 552, 753, 1248
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 6 cut(s) 508, 668, 700, 1166, 1495, 1620
BmuI ACTGGG 1 cut(s) 702
BpmI CTGGAG 2 cut(s) 135, 1230
Bpu10I CCTNAGC 2 cut(s) 1074, 1286
BpuEI CTTGAG 2 cut(s) 608, 1573
BsaAI YACGTR 3 cut(s) 368, 641, 1448
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 647, 751, 752
BsaWI WCCGGW 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
Bse3DI GCAATG 1 cut(s) 1079
BseBI CCWGG 4 cut(s) 285, 552, 753, 1248
BseDI CCNNGG 3 cut(s) 647, 751, 752
BseGI GGATG 6 cut(s) 747, 1510, 1632, 1641, 1699, 1864
BseLI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 3 cut(s) 825, 1065, 1275
BseNI ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
BseRI GAGGAG 2 cut(s) 1233, 1266
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 4 cut(s) 106, 658, 709, 1157
BseYI CCCAGC 2 cut(s) 221, 382
BshFI GGCC 7 cut(s) 322, 634, 652, 947, 971, 1201, 1661
BshNI GGYRCC 1 cut(s) 17
BsiHKAI GWGCWC 2 cut(s) 498, 1852
BsiSI CCGG 3 cut(s) 5, 242, 1778
BslFI GGGAC 4 cut(s) 294, 365, 751, 1744
BslI CCNNNNNNNGG 3 cut(s) 753, 1250, 1784
BsmAI GTCTC 3 cut(s) 85, 450, 1532
BsmFI GGGAC 4 cut(s) 294, 365, 751, 1744
BsmI GAATGC 1 cut(s) 1582
BsnI GGCC 7 cut(s) 322, 634, 652, 947, 971, 1201, 1661
Bso31I GGTCTC 1 cut(s) 85
Bsp1286I GDGCHC 3 cut(s) 22, 498, 1852
Bsp143I GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
BspACI CCGC 8 cut(s) 8, 73, 972, 1099, 1197, 1633, 1682, 1750
BspANI GGCC 7 cut(s) 322, 634, 652, 947, 971, 1201, 1661
BspCNI CTCAG 3 cut(s) 824, 1066, 1274
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 4 cut(s) 19, 282, 323, 1776
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 4 cut(s) 233, 1409, 1769, 1782
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 85
BsrBI CCGCTC 2 cut(s) 974, 1682
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 3 cut(s) 647, 751, 752
BssMI GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
BssSI CACGAG 2 cut(s) 975, 1823
BssT1I CCWWGG 1 cut(s) 647
Bst2BI CACGAG 2 cut(s) 975, 1823
Bst2UI CCWGG 4 cut(s) 285, 552, 753, 1248
Bst4CI ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
BstBAI YACGTR 3 cut(s) 368, 641, 1448
BstC8I GCNNGC 6 cut(s) 6, 260, 669, 1104, 1116, 1199
BstDEI CTNAG 5 cut(s) 811, 1074, 1261, 1286, 1534
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 6 cut(s) 747, 1510, 1632, 1641, 1699, 1864
BstHHI GCGC 2 cut(s) 933, 1573
BstKTI GATC 9 cut(s) 241, 347, 442, 1408, 1417, 1474, 1530, 1741, 1777
BstMAI GTCTC 3 cut(s) 85, 450, 1532
BstMBI GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
BstMWI GCNNNNNNNGC 8 cut(s) 72, 119, 980, 1142, 1154, 1577, 1667, 1802
BstNI CCWGG 4 cut(s) 285, 552, 753, 1248
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 283, 550, 751, 1246
BstSFI CTRYAG 2 cut(s) 1116, 1671
BstSLI GKGCMC 1 cut(s) 22
BstSNI TACGTA 1 cut(s) 641
BstV1I GCAGC 4 cut(s) 106, 658, 709, 1157
BstX2I RGATCY 3 cut(s) 344, 1738, 1774
BstYI RGATCY 3 cut(s) 344, 1738, 1774
BsuI GTATCC 2 cut(s) 565, 1121
BsuRI GGCC 7 cut(s) 322, 634, 652, 947, 971, 1201, 1661
BtsCI GGATG 6 cut(s) 747, 1510, 1632, 1641, 1699, 1864
BtsI GCAGTG 1 cut(s) 1222
BtsIMutI CAGTG 4 cut(s) 276, 690, 816, 1222
Cac8I GCNNGC 6 cut(s) 6, 260, 669, 1104, 1116, 1199
CaiI CAGNNNCTG 2 cut(s) 818, 1268
CciI TCATGA 1 cut(s) 924
CfoI GCGC 2 cut(s) 933, 1573
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 5 cut(s) 86, 281, 321, 1199, 1828
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 7 cut(s) 13, 127, 463, 638, 642, 802, 1378
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 5 cut(s) 109, 149, 925, 961, 1045
CviQI GTAC 7 cut(s) 13, 127, 463, 638, 642, 802, 1378
DdeI CTNAG 5 cut(s) 811, 1074, 1261, 1286, 1534
DpnI GATC 9 cut(s) 240, 346, 441, 1407, 1416, 1473, 1529, 1740, 1776
DpnII GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
DraIII CACNNNGTG 1 cut(s) 371
EaeI YGGCCR 3 cut(s) 945, 969, 1659
Eco105I TACGTA 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 3 cut(s) 86, 281, 1828
Eco57I CTGAAG 3 cut(s) 608, 1003, 1536
Eco72I CACGTG 2 cut(s) 368, 1448
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoO109I RGGNCCY 3 cut(s) 281, 321, 1828
EcoRII CCWGG 4 cut(s) 283, 550, 751, 1246
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 1582
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 5 cut(s) 112, 152, 928, 964, 1048
FalI AAGNNNNNCTT 6 cut(s) 387, 419, 1517, 1549, 1782, 1814
FaqI GGGAC 4 cut(s) 294, 365, 751, 1744
FatI CATG 5 cut(s) 108, 148, 924, 960, 1044
FauI CCCGC 1 cut(s) 1190
FauNDI CATATG 1 cut(s) 1591
FbaI TGATCA 2 cut(s) 439, 1405
Fnu4HI GCNGC 7 cut(s) 120, 672, 698, 972, 1100, 1146, 1683
FokI GGATG 5 cut(s) 734, 1517, 1628, 1639, 1706
Fsp4HI GCNGC 7 cut(s) 120, 672, 698, 972, 1100, 1146, 1683
FspBI CTAG 2 cut(s) 648, 1754
FspI TGCGCA 1 cut(s) 1572
GlaI GCGC 2 cut(s) 932, 1572
GluI GCNGC 7 cut(s) 120, 672, 698, 972, 1100, 1146, 1683
GsaI CCCAGC 2 cut(s) 225, 386
GsuI CTGGAG 2 cut(s) 135, 1230
HaeIII GGCC 7 cut(s) 322, 634, 652, 947, 971, 1201, 1661
HapII CCGG 3 cut(s) 5, 242, 1778
HhaI GCGC 2 cut(s) 933, 1573
Hin1II CATG 5 cut(s) 112, 152, 928, 964, 1048
Hin6I GCGC 2 cut(s) 931, 1571
HinP1I GCGC 2 cut(s) 931, 1571
HindIII AAGCTT 2 cut(s) 665, 981
HinfI GANTC 7 cut(s) 41, 188, 312, 917, 999, 1055, 1394
HpaII CCGG 3 cut(s) 5, 242, 1778
HphI GGTGA 6 cut(s) 176, 244, 362, 443, 449, 1522
Hpy166II GTNNAC 4 cut(s) 13, 235, 644, 1354
Hpy188I TCNGA 7 cut(s) 46, 489, 627, 814, 1022, 1264, 1701
Hpy8I GTNNAC 4 cut(s) 13, 235, 644, 1354
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 6 cut(s) 43, 176, 428, 562, 1511, 1778
HpyCH4III ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
HpyCH4IV ACGT 7 cut(s) 69, 367, 540, 640, 844, 1150, 1447
HpyCH4V TGCA 8 cut(s) 338, 506, 659, 1118, 1280, 1459, 1544, 1580
HpyF10VI GCNNNNNNNGC 8 cut(s) 72, 119, 980, 1142, 1154, 1577, 1667, 1802
HpyF3I CTNAG 5 cut(s) 811, 1074, 1261, 1286, 1534
HpySE526I ACGT 7 cut(s) 69, 367, 540, 640, 844, 1150, 1447
Hsp92II CATG 5 cut(s) 112, 152, 928, 964, 1048
HspAI GCGC 2 cut(s) 931, 1571
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 439, 1405
Kzo9I GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
LmnI GCTCC 2 cut(s) 116, 493
Lsp1109I GCAGC 4 cut(s) 106, 658, 709, 1157
LweI GCATC 6 cut(s) 508, 668, 700, 1166, 1495, 1620
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 2 cut(s) 648, 1754
MaeII ACGT 7 cut(s) 69, 367, 540, 640, 844, 1150, 1447
MaeIII GTNAC 5 cut(s) 532, 1031, 1219, 1270, 1709
MalI GATC 9 cut(s) 240, 346, 441, 1407, 1416, 1473, 1529, 1740, 1776
MbiI CCGCTC 2 cut(s) 974, 1682
MboI GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
MboII GAAGA 4 cut(s) 926, 1478, 1714, 1802
MfeI CAATTG 2 cut(s) 884, 1224
MflI RGATCY 3 cut(s) 344, 1738, 1774
MhlI GDGCHC 3 cut(s) 22, 498, 1852
MlsI TGGCCA 2 cut(s) 947, 1661
MluCI AATT 9 cut(s) 154, 168, 198, 288, 611, 884, 1224, 1409, 1482
MluNI TGGCCA 2 cut(s) 947, 1661
MlyI GAGTC 2 cut(s) 35, 1403
MmeI TCCRAC 2 cut(s) 124, 1513
Mox20I TGGCCA 2 cut(s) 947, 1661
Mph1103I ATGCAT 1 cut(s) 1582
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 171, 1340
MscI TGGCCA 2 cut(s) 947, 1661
MseI TTAA 2 cut(s) 1653, 1812
MslI CAYNNNNRTG 2 cut(s) 965, 1066
Msp20I TGGCCA 2 cut(s) 947, 1661
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 3 cut(s) 5, 242, 1778
MspR9I CCNGG 4 cut(s) 285, 552, 753, 1248
MunI CAATTG 2 cut(s) 884, 1224
Mva1269I GAATGC 1 cut(s) 1582
MvaI CCWGG 4 cut(s) 285, 552, 753, 1248
MwoI GCNNNNNNNGC 8 cut(s) 72, 119, 980, 1142, 1154, 1577, 1667, 1802
NaeI GCCGGC 1 cut(s) 6
NdeI CATATG 1 cut(s) 1591
NdeII GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 112, 152, 928, 964, 1048
NlaIV GGNNCC 4 cut(s) 19, 282, 323, 1776
NmuCI GTSAC 2 cut(s) 1219, 1270
NsbI TGCGCA 1 cut(s) 1572
NsiI ATGCAT 1 cut(s) 1582
NspI RCATGY 1 cut(s) 964
PagI TCATGA 1 cut(s) 924
PasI CCCWGGG 1 cut(s) 752
PceI AGGCCT 1 cut(s) 652
PctI GAATGC 1 cut(s) 1582
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 171, 1340
PfeI GAWTC 5 cut(s) 188, 312, 917, 999, 1055
PfoI TCCNGGA 1 cut(s) 1246
PkrI GCNGC 7 cut(s) 121, 673, 699, 973, 1101, 1147, 1684
PleI GAGTC 2 cut(s) 35, 1402
PmaCI CACGTG 2 cut(s) 368, 1448
PmlI CACGTG 2 cut(s) 368, 1448
PpsI GAGTC 2 cut(s) 35, 1402
Ppu21I YACGTR 3 cut(s) 368, 641, 1448
PpuMI RGGWCCY 2 cut(s) 281, 1828
Psp5II RGGWCCY 2 cut(s) 281, 1828
Psp6I CCWGG 4 cut(s) 283, 550, 751, 1246
PspCI CACGTG 2 cut(s) 368, 1448
PspFI CCCAGC 2 cut(s) 221, 382
PspGI CCWGG 4 cut(s) 283, 550, 751, 1246
PspN4I GGNNCC 4 cut(s) 19, 282, 323, 1776
PspPI GGNCC 5 cut(s) 86, 281, 321, 1199, 1828
PspPPI RGGWCCY 2 cut(s) 281, 1828
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 2 cut(s) 818, 1268
PsuI RGATCY 3 cut(s) 344, 1738, 1774
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 7 cut(s) 14, 128, 464, 639, 643, 803, 1379
RsaNI GTAC 7 cut(s) 13, 127, 463, 638, 642, 802, 1378
RseI CAYNNNNRTG 2 cut(s) 965, 1066
SaqAI TTAA 2 cut(s) 1653, 1812
SatI GCNGC 7 cut(s) 120, 672, 698, 972, 1100, 1146, 1683
Sau3AI GATC 9 cut(s) 238, 344, 439, 1405, 1414, 1471, 1527, 1738, 1774
Sau96I GGNCC 5 cut(s) 86, 281, 321, 1199, 1828
ScaI AGTACT 2 cut(s) 803, 1379
SchI GAGTC 2 cut(s) 35, 1403
ScrFI CCNGG 4 cut(s) 285, 552, 753, 1248
SduI GDGCHC 3 cut(s) 22, 498, 1852
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 6 cut(s) 508, 668, 700, 1166, 1495, 1620
SfcI CTRYAG 2 cut(s) 1116, 1671
SinI GGWCC 3 cut(s) 86, 281, 1828
SmiMI CAYNNNNRTG 2 cut(s) 965, 1066
SmlI CTYRAG 3 cut(s) 587, 1552, 1853
SmoI CTYRAG 3 cut(s) 587, 1552, 1853
SnaBI TACGTA 1 cut(s) 641
Sse9I AATT 9 cut(s) 154, 168, 198, 288, 611, 884, 1224, 1409, 1482
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 8 cut(s) 8, 73, 972, 1099, 1197, 1633, 1682, 1750
SspI AATATT 2 cut(s) 301, 560
SspMI CTAG 2 cut(s) 648, 1754
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 4 cut(s) 283, 550, 751, 1246
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 6 cut(s) 17, 472, 577, 778, 820, 1730
TaiI ACGT 7 cut(s) 72, 370, 543, 643, 847, 1153, 1450
TaqI TCGA 2 cut(s) 1066, 1373
TasI AATT 9 cut(s) 154, 168, 198, 288, 611, 884, 1224, 1409, 1482
TatI WGTACW 2 cut(s) 801, 1377
TauI GCSGC 3 cut(s) 974, 1102, 1685
TfiI GAWTC 5 cut(s) 188, 312, 917, 999, 1055
Tru1I TTAA 2 cut(s) 1653, 1812
Tru9I TTAA 2 cut(s) 1653, 1812
TscAI CASTG 4 cut(s) 276, 697, 823, 1222
TseFI GTSAC 2 cut(s) 1219, 1270
TseI GCWGC 4 cut(s) 119, 671, 697, 1145
Tsp45I GTSAC 2 cut(s) 1219, 1270
TspDTI ATGAA 5 cut(s) 552, 867, 941, 1182, 1494
TspGWI ACGGA 3 cut(s) 324, 1609, 1835
TspRI CASTG 4 cut(s) 276, 697, 823, 1222
VpaK11BI GGWCC 3 cut(s) 86, 281, 1828
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 2 cut(s) 288, 611
XceI RCATGY 1 cut(s) 964
XmaJI CCTAGG 1 cut(s) 647
XmnI GAANNNNTTC 2 cut(s) 171, 1340
XspI CTAG 2 cut(s) 648, 1754
ZrmI AGTACT 2 cut(s) 803, 1379
Zsp2I ATGCAT 1 cut(s) 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.