Rmu_sc0008619.1_g000009

V-type proton ATPase catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008619.1
OG0008079
Physical Location & Seq
Forward (+)
32671 .. 36597
3927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008619.1_g000009.1.cds

Sequence Viewer

Length: 1563 bp
atgggtggggctgccatgtacgagctggttcgtgttggaaatgataatttgattggtgaaattattcgattagaaggagattctgccacaatccaagtttatgaggagacagctgggttgatggtgaatgatcctgttctacgtacacgcaagcctttatcagtggagctgggacctggaattttgggaaatatttttgacggaatccagagacctttgaaaactattgcaaaaatatctggtgatgtatatatcccacgtggtgtgtctgtcccagctcttgacaaggatatactttgggagtttcaacctaaaaaaataggtgaaggtgatcatataactggtggagacttgtatgctactgtatttgaaaatactctgatgcagcaccatatggcgcttcctcctgatgctatgggaaaagttacatacgttgcaccagctggtcaatattcattgaaggatactgtattggagcttgagtttcaaggtgtcaaaaagcaatttactatgcttcagagttggcctgtacgtacaccaaggcctgttgcatcaaagcttgctgccgatacccctttgcttactgggcagcgtgttcttgatgcccttttcccctctgttcttggtggaacttgtgccattcctggagcatttggttgtggaaaaacagtcattagtcaagctctttccaagtattctaactcagacactgtggtttatgttggttgtggagaacgaggaaatgaaatggctgaggttcttatggattttcctcaactgacaatgactttacctgatggtcgtgaagaatctgtcatgaagcgaacaacactggttgccaacacttctaacatgcctgtggctgctcgtgaagcttcaatctatactggaatcactattgctgaatacttcagagatatgggatacaatgttagtatgatggcagattcaacatctcgatgggcagaagcattgcgtgaaatttctgggcggctggcagaaatgcctgcagatagtggatatcctgcttatttggcagcacgtttagcatcgttttatgaacgtgccggtaaagtgaaatgtcttggtggaccagagcgtactggtagtgtgactattgttggtgctgtttcaccccctggaggagatttctcagatcctgtgacatctgcaaccctcagtattgtccaggttttctggggtctggacaagaaacttgctcagaggaagcatttcccttctgtgaactggcttatttcgtactccaagtattcaacggcattagagtctttctatgataaatttgattccgactttatcaacatcaggacaaaggcacgtgaggtgttgcaaagagaagatgatctgaatgaaattgtccaagaggagaagttaaaagttaaatatggttacgtttatctgcccatgactaaaaggctgtgcatatttgcaatcttgcagcttgtaggaaaggacgctttagctgaaggagacaagatcaccttagaaactgcaaagctgttgagggaggattatctcgcacagaatgcatttactccgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0002020 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0006996 GO:0007030 GO:0007275 GO:0008150 GO:0008324 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009555 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010256 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019899 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0046961 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N ATP-synt_ab_Xtn

Protein Analysis

520

Amino Acids

57.01

Weight (kDa)

5.46

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 991
AclWI GGATC 2 cut(s) 125, 1151
AcsI RAATTY 3 cut(s) 180, 981, 1301
AcuI CTGAAG 3 cut(s) 500, 895, 1506
AcvI CACGTG 2 cut(s) 260, 1340
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 6 cut(s) 20, 145, 531, 535, 1102, 1262
AfiI CCNNNNNNNGG 1 cut(s) 1142
AgsI TTSAA 8 cut(s) 220, 308, 371, 460, 488, 879, 951, 1275
AjnI CCWGG 4 cut(s) 175, 643, 1138, 1188
Alw26I GTCTC 4 cut(s) 101, 205, 342, 1485
AlwI GGATC 2 cut(s) 125, 1151
AlwNI CAGNNNCTG 2 cut(s) 710, 1160
AoxI GGCC 2 cut(s) 524, 542
ApeKI GCWGC 7 cut(s) 11, 385, 563, 589, 863, 1037, 1459
ApoI RAATTY 3 cut(s) 180, 981, 1301
Asp700I GAANNNNTTC 2 cut(s) 63, 1232
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 2 cut(s) 173, 1091
AsuHPI GGTGA 7 cut(s) 68, 136, 254, 335, 341, 1125, 1492
AvaII GGWCC 2 cut(s) 173, 1091
BauI CACGAG 1 cut(s) 867
BbrPI CACGTG 2 cut(s) 260, 1340
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 6 cut(s) 397, 550, 601, 850, 1049, 1471
BccI CCATC 4 cut(s) 115, 791, 934, 954
BceAI ACGGC 1 cut(s) 1293
BciT130I CCWGG 4 cut(s) 177, 645, 1140, 1190
BciVI GTATCC 2 cut(s) 457, 917
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 4 cut(s) 101, 205, 342, 1485
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 401
BfuI GTATCC 2 cut(s) 457, 917
BisI GCNGC 8 cut(s) 12, 386, 564, 590, 864, 992, 1038, 1460
BlsI GCNGC 8 cut(s) 13, 387, 565, 591, 865, 993, 1039, 1461
Bme1390I CCNGG 4 cut(s) 177, 645, 1140, 1190
Bme18I GGWCC 2 cut(s) 173, 1091
BmgT120I GGNCC 2 cut(s) 173, 1091
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 4 cut(s) 177, 645, 1140, 1190
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 5 cut(s) 372, 400, 560, 592, 1058
BmuI ACTGGG 1 cut(s) 594
BpmI CTGGAG 2 cut(s) 666, 1161
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 500
BsaAI YACGTR 4 cut(s) 143, 260, 533, 1340
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 539, 1138
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 1142
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 6 cut(s) 346, 589, 837, 892, 1108, 1253
Bse3DI GCAATG 1 cut(s) 971
BseBI CCWGG 4 cut(s) 177, 645, 1140, 1190
BseDI CCNNGG 2 cut(s) 539, 1138
BseLI CCNNNNNNNGG 1 cut(s) 1142
BseMI GCAATG 1 cut(s) 971
BseMII CTCAG 5 cut(s) 717, 744, 1167, 1192, 1235
BseNI ACTGG 6 cut(s) 346, 589, 837, 892, 1108, 1253
BseRI GAGGAG 3 cut(s) 119, 1158, 1400
BseXI GCAGC 6 cut(s) 397, 550, 601, 850, 1049, 1471
BseYI CCCAGC 3 cut(s) 113, 169, 274
BshFI GGCC 2 cut(s) 526, 544
BsiSI CCGG 1 cut(s) 1068
BslFI GGGAC 2 cut(s) 186, 257
BslI CCNNNNNNNGG 1 cut(s) 1142
BsmAI GTCTC 4 cut(s) 101, 205, 342, 1485
BsmFI GGGAC 2 cut(s) 186, 257
BsmI GAATGC 1 cut(s) 1552
BsnI GGCC 2 cut(s) 526, 544
Bso31I GGTCTC 1 cut(s) 205
Bsp143I GATC 5 cut(s) 130, 331, 1156, 1363, 1497
BspACI CCGC 1 cut(s) 991
BspANI GGCC 2 cut(s) 526, 544
BspCNI CTCAG 5 cut(s) 716, 745, 1166, 1191, 1234
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 2 cut(s) 125, 1151
BspTNI GGTCTC 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 6 cut(s) 346, 589, 837, 892, 1108, 1253
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 2 cut(s) 539, 1138
BssMI GATC 5 cut(s) 130, 331, 1156, 1363, 1497
BssSI CACGAG 1 cut(s) 867
BssT1I CCWWGG 1 cut(s) 539
Bst2BI CACGAG 1 cut(s) 867
Bst2UI CCWGG 4 cut(s) 177, 645, 1140, 1190
Bst4CI ACNGT 4 cut(s) 364, 469, 670, 712
BstAPI GCANNNNNTGC 1 cut(s) 1547
BstBAI YACGTR 4 cut(s) 143, 260, 533, 1340
BstC8I GCNNGC 4 cut(s) 152, 561, 996, 1008
BstDEI CTNAG 6 cut(s) 703, 753, 1153, 1178, 1221, 1504
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 5 cut(s) 133, 334, 1159, 1366, 1500
BstMAI GTCTC 4 cut(s) 101, 205, 342, 1485
BstMBI GATC 5 cut(s) 130, 331, 1156, 1363, 1497
BstMWI GCNNNNNNNGC 5 cut(s) 586, 872, 1034, 1046, 1547
BstNI CCWGG 4 cut(s) 177, 645, 1140, 1190
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 4 cut(s) 175, 643, 1138, 1188
BstSFI CTRYAG 1 cut(s) 1008
BstSNI TACGTA 2 cut(s) 143, 533
BstV1I GCAGC 6 cut(s) 397, 550, 601, 850, 1049, 1471
BstX2I RGATCY 1 cut(s) 1156
BstYI RGATCY 1 cut(s) 1156
BsuI GTATCC 2 cut(s) 457, 917
BsuRI GGCC 2 cut(s) 526, 544
BtsIMutI CAGTG 3 cut(s) 168, 708, 830
Cac8I GCNNGC 4 cut(s) 152, 561, 996, 1008
CaiI CAGNNNCTG 2 cut(s) 710, 1160
CciI TCATGA 1 cut(s) 816
CfoI GCGC 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 2 cut(s) 173, 1091
CseI GACGC 1 cut(s) 1484
Csp6I GTAC 6 cut(s) 19, 144, 530, 534, 1101, 1261
CviAII CATG 4 cut(s) 16, 817, 853, 1426
CviQI GTAC 6 cut(s) 19, 144, 530, 534, 1101, 1261
DdeI CTNAG 6 cut(s) 703, 753, 1153, 1178, 1221, 1504
DpnI GATC 5 cut(s) 132, 333, 1158, 1365, 1499
DpnII GATC 5 cut(s) 130, 331, 1156, 1363, 1497
DraIII CACNNNGTG 1 cut(s) 263
Eco105I TACGTA 2 cut(s) 143, 533
Eco130I CCWWGG 1 cut(s) 539
Eco147I AGGCCT 1 cut(s) 544
Eco31I GGTCTC 1 cut(s) 205
Eco32I GATATC 1 cut(s) 1022
Eco47I GGWCC 2 cut(s) 173, 1091
Eco57I CTGAAG 3 cut(s) 500, 895, 1506
Eco72I CACGTG 2 cut(s) 260, 1340
EcoO109I RGGNCCY 1 cut(s) 173
EcoRII CCWGG 4 cut(s) 175, 643, 1138, 1188
EcoRV GATATC 1 cut(s) 1022
EcoT14I CCWWGG 1 cut(s) 539
EcoT22I ATGCAT 1 cut(s) 1552
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 4 cut(s) 19, 820, 856, 1429
FalI AAGNNNNNCTT 2 cut(s) 1487, 1519
FaqI GGGAC 2 cut(s) 186, 257
FatI CATG 4 cut(s) 15, 816, 852, 1425
FauNDI CATATG 1 cut(s) 393
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 8 cut(s) 12, 386, 564, 590, 864, 992, 1038, 1460
Fsp4HI GCNGC 8 cut(s) 12, 386, 564, 590, 864, 992, 1038, 1460
GlaI GCGC 1 cut(s) 399
GluI GCNGC 8 cut(s) 12, 386, 564, 590, 864, 992, 1038, 1460
GsaI CCCAGC 3 cut(s) 117, 173, 278
GsuI CTGGAG 2 cut(s) 666, 1161
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 2 cut(s) 526, 544
HapII CCGG 1 cut(s) 1068
HgaI GACGC 1 cut(s) 1484
HhaI GCGC 1 cut(s) 400
Hin1II CATG 4 cut(s) 19, 820, 856, 1429
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HindIII AAGCTT 2 cut(s) 557, 873
HinfI GANTC 7 cut(s) 80, 204, 809, 891, 947, 1286, 1307
HpaII CCGG 1 cut(s) 1068
HphI GGTGA 7 cut(s) 68, 136, 254, 335, 341, 1125, 1492
Hpy166II GTNNAC 4 cut(s) 146, 536, 1091, 1246
Hpy188I TCNGA 8 cut(s) 381, 519, 706, 914, 1156, 1224, 1312, 1368
Hpy8I GTNNAC 4 cut(s) 146, 536, 1091, 1246
HpyAV CCTTC 5 cut(s) 68, 320, 454, 1248, 1481
HpyCH4III ACNGT 4 cut(s) 364, 469, 670, 712
HpyCH4IV ACGT 8 cut(s) 142, 259, 432, 532, 1042, 1063, 1339, 1413
HpyF10VI GCNNNNNNNGC 5 cut(s) 586, 872, 1034, 1046, 1547
HpyF3I CTNAG 6 cut(s) 703, 753, 1153, 1178, 1221, 1504
HpySE526I ACGT 8 cut(s) 142, 259, 432, 532, 1042, 1063, 1339, 1413
Hsp92II CATG 4 cut(s) 19, 820, 856, 1429
HspAI GCGC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 5 cut(s) 130, 331, 1156, 1363, 1497
LmnI GCTCC 3 cut(s) 166, 475, 647
Lsp1109I GCAGC 6 cut(s) 397, 550, 601, 850, 1049, 1471
LweI GCATC 5 cut(s) 372, 400, 560, 592, 1058
MaeII ACGT 8 cut(s) 142, 259, 432, 532, 1042, 1063, 1339, 1413
MaeIII GTNAC 4 cut(s) 424, 1111, 1162, 1409
MalI GATC 5 cut(s) 132, 333, 1158, 1365, 1499
MboI GATC 5 cut(s) 130, 331, 1156, 1363, 1497
MboII GAAGA 2 cut(s) 818, 1370
MflI RGATCY 1 cut(s) 1156
MluCI AATT 7 cut(s) 46, 60, 180, 503, 981, 1301, 1374
MlyI GAGTC 1 cut(s) 1295
MmeI TCCRAC 2 cut(s) 16, 1335
Mph1103I ATGCAT 1 cut(s) 1552
MroXI GAANNNNTTC 2 cut(s) 63, 1232
MseI TTAA 2 cut(s) 1394, 1401
MslI CAYNNNNRTG 2 cut(s) 857, 958
MspA1I CMGCKG 2 cut(s) 113, 443
MspI CCGG 1 cut(s) 1068
MspR9I CCNGG 4 cut(s) 177, 645, 1140, 1190
Mva1269I GAATGC 1 cut(s) 1552
MvaI CCWGG 4 cut(s) 177, 645, 1140, 1190
MwoI GCNNNNNNNGC 5 cut(s) 586, 872, 1034, 1046, 1547
NdeI CATATG 1 cut(s) 393
NdeII GATC 5 cut(s) 130, 331, 1156, 1363, 1497
NlaIII CATG 4 cut(s) 19, 820, 856, 1429
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 1111, 1162
NsiI ATGCAT 1 cut(s) 1552
NspI RCATGY 1 cut(s) 856
PagI TCATGA 1 cut(s) 816
PceI AGGCCT 1 cut(s) 544
PctI GAATGC 1 cut(s) 1552
PdmI GAANNNNTTC 2 cut(s) 63, 1232
PfeI GAWTC 6 cut(s) 80, 204, 809, 891, 947, 1307
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 8 cut(s) 13, 387, 565, 591, 865, 993, 1039, 1461
PleI GAGTC 1 cut(s) 1294
PmaCI CACGTG 2 cut(s) 260, 1340
PmlI CACGTG 2 cut(s) 260, 1340
PpsI GAGTC 1 cut(s) 1294
Ppu21I YACGTR 4 cut(s) 143, 260, 533, 1340
PpuMI RGGWCCY 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 173
Psp6I CCWGG 4 cut(s) 175, 643, 1138, 1188
PspCI CACGTG 2 cut(s) 260, 1340
PspFI CCCAGC 3 cut(s) 113, 169, 274
PspGI CCWGG 4 cut(s) 175, 643, 1138, 1188
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 173, 1091
PspPPI RGGWCCY 1 cut(s) 173
PstI CTGCAG 1 cut(s) 1012
PstNI CAGNNNCTG 2 cut(s) 710, 1160
PsuI RGATCY 1 cut(s) 1156
PvuII CAGCTG 2 cut(s) 113, 443
RsaI GTAC 6 cut(s) 20, 145, 531, 535, 1102, 1262
RsaNI GTAC 6 cut(s) 19, 144, 530, 534, 1101, 1261
RseI CAYNNNNRTG 2 cut(s) 857, 958
SaqAI TTAA 2 cut(s) 1394, 1401
SatI GCNGC 8 cut(s) 12, 386, 564, 590, 864, 992, 1038, 1460
Sau3AI GATC 5 cut(s) 130, 331, 1156, 1363, 1497
Sau96I GGNCC 2 cut(s) 173, 1091
SchI GAGTC 1 cut(s) 1295
ScrFI CCNGG 4 cut(s) 177, 645, 1140, 1190
SfaNI GCATC 5 cut(s) 372, 400, 560, 592, 1058
SfcI CTRYAG 1 cut(s) 1008
SinI GGWCC 2 cut(s) 173, 1091
SmiMI CAYNNNNRTG 2 cut(s) 857, 958
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SnaBI TACGTA 2 cut(s) 143, 533
Sse9I AATT 7 cut(s) 46, 60, 180, 503, 981, 1301, 1374
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 1 cut(s) 991
SspI AATATT 2 cut(s) 193, 452
StuI AGGCCT 1 cut(s) 544
StyD4I CCNGG 4 cut(s) 175, 643, 1138, 1188
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 4 cut(s) 364, 469, 670, 712
TaiI ACGT 8 cut(s) 145, 262, 435, 535, 1045, 1066, 1342, 1416
TaqI TCGA 2 cut(s) 67, 958
TasI AATT 7 cut(s) 46, 60, 180, 503, 981, 1301, 1374
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 6 cut(s) 80, 204, 809, 891, 947, 1307
Tru1I TTAA 2 cut(s) 1394, 1401
Tru9I TTAA 2 cut(s) 1394, 1401
TscAI CASTG 3 cut(s) 168, 715, 837
TseFI GTSAC 2 cut(s) 1111, 1162
TseI GCWGC 7 cut(s) 11, 385, 563, 589, 863, 1037, 1459
Tsp45I GTSAC 2 cut(s) 1111, 1162
TspDTI ATGAA 5 cut(s) 444, 759, 833, 1074, 1386
TspGWI ACGGA 2 cut(s) 216, 1548
TspRI CASTG 3 cut(s) 168, 715, 837
VpaK11BI GGWCC 2 cut(s) 173, 1091
XapI RAATTY 3 cut(s) 180, 981, 1301
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmnI GAANNNNTTC 2 cut(s) 63, 1232
Zsp2I ATGCAT 1 cut(s) 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.