Rroxscaffold_2G00098030

V-type proton ATPase catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008079
Physical Location & Seq
Reverse (-)
19182743 .. 19188507
5765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098030.1

Sequence Viewer

Length: 1872 bp
ATGCCGGCGGTGTACGGTTCCCGATTGACCACATTCGAGGACTCCGAGAAGGAGAGCGAGTTTGGATACGTCCGCAAGGTCTCAGGACCAGTCGTCGTTGCAGATGGCATGGGTGGGGCTGCCATGTACGAGCTGGTTCGTGTTGGAAATGATAATTTGATTGGTGAAATTATTCGGTTAGAAGGAGATTCTGCCACAATTCAAGTTTATGAGGAGACAGCTGGGTTGATGGTGAATGATCCTGTTCTACGTACACGCAAGCCTTTATCAGTGGAGCTGGGACCTGGAATTTTGGGAAATATTTTTGACGGAATCCAGAGACCTTTGAAAACTATTGCAAAAATATCTGGTGATGTCTATATCCCACGTGGTGTGTCTGTCCCAGCTCTTGACAAGGATATACTTTGGGAGTTTCAACCTAAAAAAATAGGTGAAGGTGATCATATAACTGGTGGAGACTTGTATGCTACTGTGTTTGAAAATACTCTGATGCAGCACCATATGGCGCTTCCTCCTGATGCTATGGGAAAAGTTACATATGTTGCACCAGCTGGTCAATATTCATTGAAGGATACTGTATTGGAGCTTGAGTTTCAAGGTGTCAAAAAGCAATTTACTATGCTTCAGAGTTGGCCTGTACGTACACCAAGGCCTGTCGCATCAAAGCTTGCTGCCGATACCCCTTTGCTTACTGGGCAGCGTGTTCTTGATGCCCTTTTCCCCTCTGTTCTTGGTGGAACTTGTGCCATTCCTGGAGCATTTGGTTGTGGAAAAACAGTCATTAGTCAAGCTCTTTCCAAGTATTCTAACTCAGACACTGTGGTTTATGTTGGTTGTGGAGAACGAGGAAATGAAATGGCTGAGGTTCTTATGGATTTTCCTCAACTGACAATGACTTTACCTGATGGTCGTGAAGAATCTGTCATGAAGCGAACAACACTGGTCGCCAACACTTCTAACATGCCTGTGGCTGCTCGTGAAGCTTCAATCTATACTGGAATCACTATTGCTGAATACTTCAGAGATATGGGATACAATGTTAGTATGATGGCAGATTCAACATCTCGATGGGCAGAAGCATTGCGTGAAATTTCTGGGCGGCTGGCAGAAATGCCTGCAGATAGTGGATATCCTGCTTATTTGGCAGCACGTTTAGCATCGTTTTATGAACGTGCCGGTAAAGTAAAATGTCTTGGTGGACCAGAGCGTACTGGTAGTGTGACTATTGTTGGTGCTGTTTCACCCCCTGGAGGAGATTTCTCAGATCCTGTGACATCTGCAACCCTCAGTATTGTCCAGGTTTTCTGGGGTCTGGACAAGAAACTTGCTCAGAGGAAGCATTTCCCTTCTGTGAACTGGCTTATTTCGTACTCCAAGTATTCAACGGCATTAGAGTCTTTCTATGATAAATTTGATTCCGACTTTATCAACATCAGGACAAAGGCACGTGAGGTGTTGCAAAGAGAAGATGATCTGAATGAAATTGTCCAACTTGTAGGAAAGGATGCTTTAGCTGAAGGAGACAAGATCACCTTAGAAACTGCAAAGCTGTTGAGGGAGGATTATCTCGCACAGAATGCATTTACTCCATATGATAAATTCTGCCCCTTCTACAAGTCTGTTTGGATGATGCGGAATATCATCCATTTCTTTAATTTGGCAAATCAGGCAGTAGAGAGAGCAGCTGGTATGGATGGTCAAAAGATTAATTACAGTCTAATCAAGCACCGTATGGGAGATCTCTTTTACCGCCTAGTGTCTCAAAAATTTGAGGATCCTGCTGAAGGTGAACAAGCGCTTGTGGCAAAATTTAAGAAGCTTCATGAAGACTTAACGAACGGTTTCCGTGCTCTTGAGGATGAAACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0002020 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0006996 GO:0007030 GO:0007275 GO:0008150 GO:0008324 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009555 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010256 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019899 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0046961 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N ATP-synt_ab_Xtn

Protein Analysis

623

Amino Acids

68.74

Weight (kDa)

5.42

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 25 - 85 2.7e-15 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab_Xtn PF16886 127 - 214 8e-28 ATPsynthase alpha/beta subunit barrel-sandwich domain
ATP-synt_ab PF00006 232 - 458 1.2e-74 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_VA_C PF22919 466 - 560 2.5e-28 C-terminal domain of V and A type ATP synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 8, 73, 1099, 1633, 1750
AclWI GGATC 4 cut(s) 233, 1259, 1769, 1782
AcsI RAATTY 6 cut(s) 288, 1089, 1409, 1598, 1766, 1808
AcuI CTGAAG 4 cut(s) 608, 1003, 1536, 1803
AcvI CACGTG 2 cut(s) 368, 1448
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 7 cut(s) 14, 128, 253, 639, 643, 1210, 1370
AfeI AGCGCT 1 cut(s) 1797
AfiI CCNNNNNNNGG 2 cut(s) 1250, 1784
AgsI TTSAA 9 cut(s) 203, 328, 416, 479, 568, 596, 987, 1059, 1383
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 4 cut(s) 283, 751, 1246, 1296
Alw21I GWGCWC 1 cut(s) 1852
Alw26I GTCTC 6 cut(s) 85, 209, 313, 450, 1515, 1764
AlwI GGATC 4 cut(s) 233, 1259, 1769, 1782
AlwNI CAGNNNCTG 2 cut(s) 818, 1268
Aor51HI AGCGCT 1 cut(s) 1797
AoxI GGCC 2 cut(s) 632, 650
ApeKI GCWGC 7 cut(s) 119, 493, 671, 697, 971, 1145, 1682
ApoI RAATTY 6 cut(s) 288, 1089, 1409, 1598, 1766, 1808
AseI ATTAAT 1 cut(s) 1707
Asp700I GAANNNNTTC 3 cut(s) 171, 1340, 1841
AspLEI GCGC 2 cut(s) 508, 1798
AspS9I GGNCC 3 cut(s) 86, 281, 1199
AsuHPI GGTGA 8 cut(s) 176, 244, 362, 443, 449, 1233, 1522, 1799
AvaII GGWCC 3 cut(s) 86, 281, 1199
BamHI GGATCC 1 cut(s) 1774
BauI CACGAG 1 cut(s) 975
BbrPI CACGTG 2 cut(s) 368, 1448
BbsI GAAGAC 1 cut(s) 1833
Bbv12I GWGCWC 1 cut(s) 1852
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 7 cut(s) 106, 505, 658, 709, 958, 1157, 1694
BccI CCATC 6 cut(s) 98, 223, 899, 1042, 1062, 1688
BceAI ACGGC 1 cut(s) 1401
BciT130I CCWGG 4 cut(s) 285, 753, 1248, 1298
BciVI GTATCC 3 cut(s) 59, 565, 1025
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 6 cut(s) 85, 209, 313, 450, 1515, 1764
BfaI CTAG 1 cut(s) 1754
BfmI CTRYAG 1 cut(s) 1116
BfoI RGCGCY 2 cut(s) 509, 1799
BfuI GTATCC 3 cut(s) 59, 565, 1025
BglII AGATCT 1 cut(s) 1738
BisI GCNGC 8 cut(s) 120, 494, 672, 698, 972, 1100, 1146, 1683
BlsI GCNGC 8 cut(s) 121, 495, 673, 699, 973, 1101, 1147, 1684
Bme1390I CCNGG 4 cut(s) 285, 753, 1248, 1298
Bme18I GGWCC 3 cut(s) 86, 281, 1199
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 86, 281, 1199
BmiI GGNNCC 3 cut(s) 19, 282, 1776
BmrFI CCNGG 4 cut(s) 285, 753, 1248, 1298
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 7 cut(s) 480, 508, 668, 700, 1166, 1495, 1620
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 1833
BpmI CTGGAG 2 cut(s) 774, 1269
Bpu10I CCTNAGC 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 608
BsaAI YACGTR 4 cut(s) 251, 368, 641, 1448
BsaI GGTCTC 2 cut(s) 85, 313
BsaJI CCNNGG 2 cut(s) 647, 1246
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 1250, 1784
Bse118I RCCGGY 2 cut(s) 4, 1175
Bse1I ACTGG 7 cut(s) 89, 454, 697, 945, 1000, 1216, 1361
Bse3DI GCAATG 1 cut(s) 1079
BseBI CCWGG 4 cut(s) 285, 753, 1248, 1298
BseDI CCNNGG 2 cut(s) 647, 1246
BseGI GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BseLI CCNNNNNNNGG 2 cut(s) 1250, 1784
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 6 cut(s) 96, 825, 852, 1275, 1300, 1343
BseNI ACTGG 7 cut(s) 89, 454, 697, 945, 1000, 1216, 1361
BseRI GAGGAG 2 cut(s) 227, 1266
BseXI GCAGC 7 cut(s) 106, 505, 658, 709, 958, 1157, 1694
BseYI CCCAGC 3 cut(s) 221, 277, 382
BshFI GGCC 2 cut(s) 634, 652
BsiHKAI GWGCWC 1 cut(s) 1852
BsiSI CCGG 2 cut(s) 5, 1176
BslFI GGGAC 2 cut(s) 294, 365
BslI CCNNNNNNNGG 2 cut(s) 1250, 1784
BsmAI GTCTC 6 cut(s) 85, 209, 313, 450, 1515, 1764
BsmFI GGGAC 2 cut(s) 294, 365
BsmI GAATGC 1 cut(s) 1582
BsnI GGCC 2 cut(s) 634, 652
Bso31I GGTCTC 2 cut(s) 85, 313
Bsp1286I GDGCHC 1 cut(s) 1852
Bsp143I GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
BspACI CCGC 5 cut(s) 8, 73, 1099, 1633, 1750
BspANI GGCC 2 cut(s) 634, 652
BspCNI CTCAG 6 cut(s) 95, 824, 853, 1274, 1299, 1342
BspHI TCATGA 2 cut(s) 924, 1822
BspLI GGNNCC 3 cut(s) 19, 282, 1776
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 4 cut(s) 233, 1259, 1769, 1782
BspTNI GGTCTC 2 cut(s) 85, 313
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 2 cut(s) 4, 1175
BsrI ACTGG 7 cut(s) 89, 454, 697, 945, 1000, 1216, 1361
BssAI RCCGGY 2 cut(s) 4, 1175
BssECI CCNNGG 2 cut(s) 647, 1246
BssMI GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
BssSI CACGAG 1 cut(s) 975
BssT1I CCWWGG 1 cut(s) 647
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 4 cut(s) 285, 753, 1248, 1298
Bst4CI ACNGT 8 cut(s) 17, 472, 577, 778, 820, 1715, 1730, 1841
BstAPI GCANNNNNTGC 1 cut(s) 1577
BstBAI YACGTR 4 cut(s) 251, 368, 641, 1448
BstC8I GCNNGC 5 cut(s) 6, 260, 669, 1104, 1116
BstDEI CTNAG 7 cut(s) 82, 811, 861, 1261, 1286, 1329, 1534
BstENI CCTNNNNNAGG 1 cut(s) 1782
BstF5I GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BstH2I RGCGCY 2 cut(s) 509, 1799
BstHHI GCGC 2 cut(s) 508, 1798
BstKTI GATC 7 cut(s) 241, 442, 1267, 1474, 1530, 1741, 1777
BstMAI GTCTC 6 cut(s) 85, 209, 313, 450, 1515, 1764
BstMBI GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
BstMWI GCNNNNNNNGC 7 cut(s) 694, 980, 1142, 1154, 1577, 1667, 1802
BstNI CCWGG 4 cut(s) 285, 753, 1248, 1298
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 283, 751, 1246, 1296
BstSFI CTRYAG 1 cut(s) 1116
BstSNI TACGTA 2 cut(s) 251, 641
BstV1I GCAGC 7 cut(s) 106, 505, 658, 709, 958, 1157, 1694
BstV2I GAAGAC 1 cut(s) 1833
BstX2I RGATCY 3 cut(s) 1264, 1738, 1774
BstYI RGATCY 3 cut(s) 1264, 1738, 1774
BsuI GTATCC 3 cut(s) 59, 565, 1025
BsuRI GGCC 2 cut(s) 634, 652
BtsCI GGATG 5 cut(s) 1510, 1632, 1641, 1699, 1864
BtsIMutI CAGTG 3 cut(s) 276, 816, 938
Cac8I GCNNGC 5 cut(s) 6, 260, 669, 1104, 1116
CaiI CAGNNNCTG 2 cut(s) 818, 1268
CciI TCATGA 2 cut(s) 924, 1822
CfoI GCGC 2 cut(s) 508, 1798
Cfr10I RCCGGY 2 cut(s) 4, 1175
Cfr13I GGNCC 3 cut(s) 86, 281, 1199
Csp6I GTAC 7 cut(s) 13, 127, 252, 638, 642, 1209, 1369
CviAII CATG 5 cut(s) 109, 124, 925, 961, 1823
CviQI GTAC 7 cut(s) 13, 127, 252, 638, 642, 1209, 1369
DdeI CTNAG 7 cut(s) 82, 811, 861, 1261, 1286, 1329, 1534
DpnI GATC 7 cut(s) 240, 441, 1266, 1473, 1529, 1740, 1776
DpnII GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
DraIII CACNNNGTG 1 cut(s) 371
DriI GACNNNNNGTC 1 cut(s) 92
Eam1105I GACNNNNNGTC 1 cut(s) 92
Eco105I TACGTA 2 cut(s) 251, 641
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 652
Eco31I GGTCTC 2 cut(s) 85, 313
Eco32I GATATC 1 cut(s) 1130
Eco47I GGWCC 3 cut(s) 86, 281, 1199
Eco47III AGCGCT 1 cut(s) 1797
Eco57I CTGAAG 4 cut(s) 608, 1003, 1536, 1803
Eco72I CACGTG 2 cut(s) 368, 1448
EcoNI CCTNNNNNAGG 1 cut(s) 1782
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 4 cut(s) 283, 751, 1246, 1296
EcoRV GATATC 1 cut(s) 1130
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 1582
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 5 cut(s) 112, 127, 928, 964, 1826
FalI AAGNNNNNCTT 2 cut(s) 1517, 1549
FaqI GGGAC 2 cut(s) 294, 365
FatI CATG 5 cut(s) 108, 123, 924, 960, 1822
FauNDI CATATG 3 cut(s) 501, 538, 1591
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 8 cut(s) 120, 494, 672, 698, 972, 1100, 1146, 1683
FokI GGATG 4 cut(s) 1517, 1628, 1639, 1706
Fsp4HI GCNGC 8 cut(s) 120, 494, 672, 698, 972, 1100, 1146, 1683
FspBI CTAG 1 cut(s) 1754
GlaI GCGC 2 cut(s) 507, 1797
GluI GCNGC 8 cut(s) 120, 494, 672, 698, 972, 1100, 1146, 1683
GsaI CCCAGC 3 cut(s) 225, 281, 386
GsuI CTGGAG 2 cut(s) 774, 1269
HaeII RGCGCY 2 cut(s) 509, 1799
HaeIII GGCC 2 cut(s) 634, 652
HapII CCGG 2 cut(s) 5, 1176
HhaI GCGC 2 cut(s) 508, 1798
Hin1II CATG 5 cut(s) 112, 127, 928, 964, 1826
Hin6I GCGC 2 cut(s) 506, 1796
HinP1I GCGC 2 cut(s) 506, 1796
HindIII AAGCTT 3 cut(s) 665, 981, 1817
HinfI GANTC 8 cut(s) 41, 188, 312, 917, 999, 1055, 1394, 1415
HpaII CCGG 2 cut(s) 5, 1176
HphI GGTGA 8 cut(s) 176, 244, 362, 443, 449, 1233, 1522, 1799
Hpy166II GTNNAC 6 cut(s) 13, 254, 644, 1199, 1354, 1790
Hpy188I TCNGA 9 cut(s) 46, 489, 627, 814, 1022, 1264, 1332, 1420, 1476
Hpy8I GTNNAC 6 cut(s) 13, 254, 644, 1199, 1354, 1790
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 8 cut(s) 43, 176, 428, 562, 1356, 1511, 1618, 1778
HpyCH4III ACNGT 8 cut(s) 17, 472, 577, 778, 820, 1715, 1730, 1841
HpyCH4IV ACGT 7 cut(s) 69, 250, 367, 640, 1150, 1171, 1447
HpyCH4V TGCA 9 cut(s) 101, 338, 493, 545, 1118, 1280, 1459, 1544, 1580
HpyF10VI GCNNNNNNNGC 7 cut(s) 694, 980, 1142, 1154, 1577, 1667, 1802
HpyF3I CTNAG 7 cut(s) 82, 811, 861, 1261, 1286, 1329, 1534
HpySE526I ACGT 7 cut(s) 69, 250, 367, 640, 1150, 1171, 1447
Hsp92II CATG 5 cut(s) 112, 127, 928, 964, 1826
HspAI GCGC 2 cut(s) 506, 1796
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
LmnI GCTCC 3 cut(s) 274, 583, 755
Lsp1109I GCAGC 7 cut(s) 106, 505, 658, 709, 958, 1157, 1694
LweI GCATC 7 cut(s) 480, 508, 668, 700, 1166, 1495, 1620
MaeI CTAG 1 cut(s) 1754
MaeII ACGT 7 cut(s) 69, 250, 367, 640, 1150, 1171, 1447
MaeIII GTNAC 3 cut(s) 532, 1219, 1270
MalI GATC 7 cut(s) 240, 441, 1266, 1473, 1529, 1740, 1776
MboI GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
MboII GAAGA 3 cut(s) 926, 1478, 1838
MflI RGATCY 3 cut(s) 1264, 1738, 1774
MhlI GDGCHC 1 cut(s) 1852
MlyI GAGTC 2 cut(s) 35, 1403
MmeI TCCRAC 3 cut(s) 124, 1443, 1513
Mph1103I ATGCAT 1 cut(s) 1582
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 3 cut(s) 171, 1340, 1841
MseI TTAA 4 cut(s) 1653, 1707, 1812, 1832
MslI CAYNNNNRTG 2 cut(s) 965, 1066
MspA1I CMGCKG 3 cut(s) 221, 551, 1685
MspI CCGG 2 cut(s) 5, 1176
MspR9I CCNGG 4 cut(s) 285, 753, 1248, 1298
Mva1269I GAATGC 1 cut(s) 1582
MvaI CCWGG 4 cut(s) 285, 753, 1248, 1298
MwoI GCNNNNNNNGC 7 cut(s) 694, 980, 1142, 1154, 1577, 1667, 1802
NaeI GCCGGC 1 cut(s) 6
NdeI CATATG 3 cut(s) 501, 538, 1591
NdeII GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 112, 127, 928, 964, 1826
NlaIV GGNNCC 3 cut(s) 19, 282, 1776
NmuCI GTSAC 2 cut(s) 1219, 1270
NsiI ATGCAT 1 cut(s) 1582
NspI RCATGY 1 cut(s) 964
PagI TCATGA 2 cut(s) 924, 1822
PceI AGGCCT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 1 cut(s) 1582
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 3 cut(s) 171, 1340, 1841
PfeI GAWTC 6 cut(s) 188, 312, 917, 999, 1055, 1415
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 8 cut(s) 121, 495, 673, 699, 973, 1101, 1147, 1684
PleI GAGTC 2 cut(s) 35, 1402
PmaCI CACGTG 2 cut(s) 368, 1448
PmlI CACGTG 2 cut(s) 368, 1448
PpsI GAGTC 2 cut(s) 35, 1402
Ppu21I YACGTR 4 cut(s) 251, 368, 641, 1448
PpuMI RGGWCCY 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 1707
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 4 cut(s) 283, 751, 1246, 1296
PspCI CACGTG 2 cut(s) 368, 1448
PspFI CCCAGC 3 cut(s) 221, 277, 382
PspGI CCWGG 4 cut(s) 283, 751, 1246, 1296
PspN4I GGNNCC 3 cut(s) 19, 282, 1776
PspPI GGNCC 3 cut(s) 86, 281, 1199
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 2 cut(s) 818, 1268
PsuI RGATCY 3 cut(s) 1264, 1738, 1774
PvuII CAGCTG 3 cut(s) 221, 551, 1685
RsaI GTAC 7 cut(s) 14, 128, 253, 639, 643, 1210, 1370
RsaNI GTAC 7 cut(s) 13, 127, 252, 638, 642, 1209, 1369
RseI CAYNNNNRTG 2 cut(s) 965, 1066
SaqAI TTAA 4 cut(s) 1653, 1707, 1812, 1832
SatI GCNGC 8 cut(s) 120, 494, 672, 698, 972, 1100, 1146, 1683
Sau3AI GATC 7 cut(s) 238, 439, 1264, 1471, 1527, 1738, 1774
Sau96I GGNCC 3 cut(s) 86, 281, 1199
SchI GAGTC 2 cut(s) 35, 1403
ScrFI CCNGG 4 cut(s) 285, 753, 1248, 1298
SduI GDGCHC 1 cut(s) 1852
SfaNI GCATC 7 cut(s) 480, 508, 668, 700, 1166, 1495, 1620
SfcI CTRYAG 1 cut(s) 1116
SinI GGWCC 3 cut(s) 86, 281, 1199
SmiMI CAYNNNNRTG 2 cut(s) 965, 1066
SmlI CTYRAG 2 cut(s) 587, 1853
SmoI CTYRAG 2 cut(s) 587, 1853
SnaBI TACGTA 2 cut(s) 251, 641
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 5 cut(s) 8, 73, 1099, 1633, 1750
SspI AATATT 2 cut(s) 301, 560
SspMI CTAG 1 cut(s) 1754
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 4 cut(s) 283, 751, 1246, 1296
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 8 cut(s) 17, 472, 577, 778, 820, 1715, 1730, 1841
TaiI ACGT 7 cut(s) 72, 253, 370, 643, 1153, 1174, 1450
TaqI TCGA 3 cut(s) 36, 1066, 1867
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 6 cut(s) 188, 312, 917, 999, 1055, 1415
Tru1I TTAA 4 cut(s) 1653, 1707, 1812, 1832
Tru9I TTAA 4 cut(s) 1653, 1707, 1812, 1832
TscAI CASTG 3 cut(s) 276, 823, 945
TseFI GTSAC 2 cut(s) 1219, 1270
TseI GCWGC 7 cut(s) 119, 493, 671, 697, 971, 1145, 1682
Tsp45I GTSAC 2 cut(s) 1219, 1270
TspDTI ATGAA 7 cut(s) 552, 867, 941, 1182, 1494, 1811, 1839
TspGWI ACGGA 2 cut(s) 324, 1835
TspRI CASTG 3 cut(s) 276, 823, 945
VpaK11BI GGWCC 3 cut(s) 86, 281, 1199
VspI ATTAAT 1 cut(s) 1707
XagI CCTNNNNNAGG 1 cut(s) 1782
XapI RAATTY 6 cut(s) 288, 1089, 1409, 1598, 1766, 1808
XceI RCATGY 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmnI GAANNNNTTC 3 cut(s) 171, 1340, 1841
XspI CTAG 1 cut(s) 1754
Zsp2I ATGCAT 1 cut(s) 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.