Rorug02G0412100

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002762
Physical Location & Seq
Reverse (-)
52807297 .. 52813134
5838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0412100.1

Sequence Viewer

Length: 2106 bp
ATGGTGAGCTCAGACGGATTGAGTGCTTCTCCGCCGCCGCCGCCGCAGGCGGTGTCGGCAAAGCAATTCGGGGTCACAAAGCCGATTTCGACGGCCGGGCCTACTGAGGCCGATACTCAGAGAACTTTAGAGTTGGAGAAGCTTTTGGTTGAAGCTGGGCTTTACGAAAGCAAAGGGGAAGCTGCCAAGAGAGAGGAGGTTCTTCACCGAATTGGGCAGATTGTTAAGGATTGGGTAAAGCAACTTACTCAGTTGAGAGGATACACAGATCAAATGGTTGAGGATGCTAATGCTCTTATTTTTACATTTGGTTCCTATAGGCTTGGGGTACATGGTCCTGGGGCTGACATAGACACATTGTGTGTTGGGCCATCTTATGTCAACCGTGAGGAAGACTTCTTCTTTGTATTGCACAACATTCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCCCATGTTCCCGTAATGAAGTTTAAGTTTGATGGAATATCAATCGACCTCCTTTATGCAAGTGTTTCTCTGTTGGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTACAACATCGATGAGCCTACCGTTCGAAGTCTTAATGGCTGCAGGGTTGCTGATCAAATTCTTAAGCTTGTTCCAAATGTTGAGCATTTCTGTACAACACTCCGATGCTTGAAGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTACTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTTGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGTTCCTAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCTATTGAAGAGGATGAACTTGGTTTTAATGTTTGGGACCCACGCAAAAATCCTCGCGACCGTACTCATCATATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAGTTTCAGCAGGGAAACAAGATTTGTGAGGAAATAGAGCTGAATAAAGCTAGGTGGTGTGCTCTGTTTGAACCATATTTGTTCTTTGAAAGCTACAAGAACTATCTCCAGGTCGACATCGTTGCAGTTGATGCTGATGATCTGCGTGCTTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATACTTTGGAGATGTTACAATGTCACCCATATCCTCATGAGTATGTTGATACGACCAAACAGTGTGCACATAGTGCTTTCTTTATGGGTTTGCAGAGGAAACAAGGTGAAAAGGTTCAGGGTCAGCAGTTCGATATACGAGGGACTGTTGATGAGTTCAAGCAGTCCATTGAGATGTACAATTTTTGGAAACCTGGGATGGAAATTTATGTTTCTCACGTTCGTAGAAGGCAACTTCCTTCTTATGTGTTTCCTGATGGTTGTAAACGACCTAGACCATCTAGAGCTGTGGCCCTGCAGCATGATAATGAAGTTAGTCCGACGGGTTCTGGTGAGAGACACCTGAAGAGGAAGAATGATCTTGGTGGGGGTTCGCCACCGAAACGACTATCTATTAGCCCTCTGCCCCAGGATTCAATATCTCCTGAAATTATCACTCACAGAGGAGGAAGTGCATCTCCAGAGACTTTTGATAAATGCTTTAGTTTGGAGGTAGTTGATTCAAATAAAAAAACGTTGTCCGGTGAACTGGAGCCTGGAGATGCGACCAATTCCAGTGTTGTTACAAGTGTTACAAGCATGAGCAGTAGTGAAGATGCTGGATTTGGATCAATGACTGGTAGCTGTGAGGGGAATACTAAAAGTGTTGAAGGAAGCAGTATTGGGAGTAACACTGCTTCTTTATGTGAGGCAGACTCTGAATCTCTTTCAGTTAACGGAGGCGAAAATGGCAACCTTGTGCAAAGTGGATCACAAGAAGGATTAGAGTTGAATGCTGCATTTGGGATGATACTTAATCCTAGCGAGGGAGTAAGATCGGAAGGTGTGCAGAAAGCAGTGATGAGCAGGTTGGGTTTAACCTCAATGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
DUF1754 ERG4_ERG24

Protein Analysis

701

Amino Acids

77.95

Weight (kDa)

5.39

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 24 - 218 2.5e-77 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 84 - 173 5e-11 Nucleotidyltransferase domain
PAP_central PF04928 223 - 367 7.7e-70 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 371 - 430 7.7e-11 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 428 - 503 6.2e-08 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
Acc36I ACCTGC 1 cut(s) 2070
AccBSI CCGCTC 1 cut(s) 1231
AccI GTMKAC 2 cut(s) 837, 1143
AccII CGCG 1 cut(s) 933
AciI CCGC 7 cut(s) 32, 35, 38, 41, 44, 50, 1231
AclI AACGTT 1 cut(s) 1748
AclWI GGATC 2 cut(s) 1849, 1990
AcoI YGGCCR 2 cut(s) 93, 854
AcsI RAATTY 2 cut(s) 639, 1437
AcuI CTGAAG 1 cut(s) 1598
AdeI CACNNNGTG 3 cut(s) 360, 846, 1307
AfaI GTAC 7 cut(s) 330, 546, 584, 676, 940, 1009, 1412
AfiI CCNNNNNNNGG 1 cut(s) 2039
AflII CTTAAG 1 cut(s) 644
AjnI CCWGG 6 cut(s) 337, 555, 1137, 1426, 1641, 1768
Alw21I GWGCWC 3 cut(s) 11, 1093, 1303
Alw26I GTCTC 2 cut(s) 1564, 1691
Alw44I GTGCAC 1 cut(s) 1299
AlwI GGATC 2 cut(s) 1849, 1990
AlwNI CAGNNNCTG 1 cut(s) 1931
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 7 cut(s) 93, 98, 108, 368, 854, 1524, 2100
ApaLI GTGCAC 1 cut(s) 1299
ApeKI GCWGC 4 cut(s) 182, 621, 1531, 2009
ApoI RAATTY 2 cut(s) 639, 1437
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp700I GAANNNNTTC 1 cut(s) 1347
AspS9I GGNCC 5 cut(s) 98, 335, 368, 913, 1525
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 6 cut(s) 16, 197, 1250, 1352, 1577, 1769
AsuII TTCGAA 1 cut(s) 607
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 2 cut(s) 335, 913
BaeGI GKGCMC 1 cut(s) 1303
BalI TGGCCA 1 cut(s) 856
BanII GRGCYC 2 cut(s) 11, 763
BarI GAAGNNNNNNTAC 2 cut(s) 1894, 1926
BbsI GAAGAC 2 cut(s) 399, 558
Bbv12I GWGCWC 3 cut(s) 11, 1093, 1303
BbvI GCAGC 4 cut(s) 169, 608, 1543, 1996
BccI CCATC 7 cut(s) 379, 491, 846, 1018, 1426, 1484, 1519
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 2 cut(s) 1133, 1167
BciT130I CCWGG 6 cut(s) 339, 557, 1139, 1428, 1643, 1770
BciVI GTATCC 2 cut(s) 254, 1228
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 1564, 1691
BfaI CTAG 6 cut(s) 807, 987, 1080, 1506, 1515, 2034
BfmI CTRYAG 3 cut(s) 316, 622, 1529
BfrI CTTAAG 1 cut(s) 644
BfuAI ACCTGC 1 cut(s) 2070
BfuI GTATCC 2 cut(s) 254, 1228
BisI GCNGC 8 cut(s) 35, 38, 41, 44, 183, 622, 1532, 2010
BlsI GCNGC 8 cut(s) 36, 39, 42, 45, 184, 623, 1533, 2011
Bme1390I CCNGG 7 cut(s) 97, 339, 557, 1139, 1428, 1643, 1770
Bme18I GGWCC 2 cut(s) 335, 913
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 5 cut(s) 98, 335, 368, 913, 1525
BmiI GGNNCC 4 cut(s) 313, 914, 915, 1767
BmrFI CCNGG 7 cut(s) 97, 339, 557, 1139, 1428, 1643, 1770
BmrI ACTGGG 1 cut(s) 766
BmsI GCATC 7 cut(s) 274, 454, 677, 1150, 1697, 1765, 1819
BmuI ACTGGG 1 cut(s) 766
BpiI GAAGAC 2 cut(s) 399, 558
BplI GAGNNNNNCTC 4 cut(s) 13, 45, 1913, 1945
BpmI CTGGAG 4 cut(s) 1121, 1677, 1784, 1791
Bpu14I TTCGAA 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 97
Bsa29I ATCGAT 1 cut(s) 591
BsaBI GATNNNNATC 1 cut(s) 1840
BsaJI CCNNGG 3 cut(s) 338, 1427, 1641
BsaWI WCCGGW 1 cut(s) 1754
Bsc4I CCNNNNNNNGG 1 cut(s) 2039
Bse1I ACTGG 6 cut(s) 455, 739, 761, 1767, 1788, 1855
Bse8I GATNNNNATC 1 cut(s) 1840
BseBI CCWGG 6 cut(s) 339, 557, 1139, 1428, 1643, 1770
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 3 cut(s) 338, 1427, 1641
BseGI GGATG 4 cut(s) 289, 895, 1437, 2025
BseJI GATNNNNATC 1 cut(s) 1840
BseLI CCNNNNNNNGG 1 cut(s) 2039
BseMII CTCAG 4 cut(s) 24, 96, 131, 263
BseNI ACTGG 6 cut(s) 455, 739, 761, 1767, 1788, 1855
BseRI GAGGAG 2 cut(s) 209, 1692
BseSI GKGCMC 1 cut(s) 1303
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 4 cut(s) 169, 608, 1543, 1996
BseYI CCCAGC 2 cut(s) 155, 1188
BsgI GTGCAG 1 cut(s) 2081
Bsh1236I CGCG 1 cut(s) 933
Bsh1285I CGRYCG 2 cut(s) 96, 937
BshFI GGCC 7 cut(s) 95, 100, 110, 370, 856, 1526, 2102
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 2 cut(s) 96, 937
BsiHKAI GWGCWC 3 cut(s) 11, 1093, 1303
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 2 cut(s) 96, 1755
BslFI GGGAC 2 cut(s) 926, 1390
BslI CCNNNNNNNGG 1 cut(s) 2039
BsmAI GTCTC 2 cut(s) 1564, 1691
BsmFI GGGAC 2 cut(s) 926, 1390
BsmI GAATGC 1 cut(s) 2011
BsnI GGCC 7 cut(s) 95, 100, 110, 370, 856, 1526, 2102
BsoBI CYCGRG 1 cut(s) 774
Bsp119I TTCGAA 1 cut(s) 607
Bsp1286I GDGCHC 4 cut(s) 11, 763, 1093, 1303
Bsp1407I TGTACA 3 cut(s) 582, 674, 1410
Bsp143I GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
Bsp68I TCGCGA 1 cut(s) 933
BspACI CCGC 7 cut(s) 32, 35, 38, 41, 44, 50, 1231
BspANI GGCC 7 cut(s) 95, 100, 110, 370, 856, 1526, 2102
BspCNI CTCAG 4 cut(s) 23, 97, 130, 262
BspDI ATCGAT 1 cut(s) 591
BspFNI CGCG 1 cut(s) 933
BspHI TCATGA 2 cut(s) 1020, 1270
BspLI GGNNCC 4 cut(s) 313, 914, 915, 1767
BspMAI CTGCAG 2 cut(s) 626, 1533
BspMI ACCTGC 1 cut(s) 2070
BspPI GGATC 2 cut(s) 1849, 1990
BspT104I TTCGAA 1 cut(s) 607
BspTI CTTAAG 1 cut(s) 644
BsrBI CCGCTC 1 cut(s) 1231
BsrGI TGTACA 3 cut(s) 582, 674, 1410
BsrI ACTGG 6 cut(s) 455, 739, 761, 1767, 1788, 1855
BssECI CCNNGG 3 cut(s) 338, 1427, 1641
BssMI GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
Bst2UI CCWGG 6 cut(s) 339, 557, 1139, 1428, 1643, 1770
Bst4CI ACNGT 6 cut(s) 386, 604, 846, 938, 1296, 1381
Bst6I CTCTTC 2 cut(s) 879, 1574
BstAFI CTTAAG 1 cut(s) 644
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1307
BstAUI TGTACA 3 cut(s) 582, 674, 1410
BstBI TTCGAA 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 48, 812, 1176
BstDEI CTNAG 4 cut(s) 10, 105, 117, 249
BstF5I GGATG 4 cut(s) 289, 895, 1437, 2025
BstFNI CGCG 1 cut(s) 933
BstKTI GATC 7 cut(s) 271, 637, 1171, 1594, 1844, 1985, 2051
BstMAI GTCTC 2 cut(s) 1564, 1691
BstMBI GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
BstMCI CGRYCG 2 cut(s) 96, 937
BstMWI GCNNNNNNNGC 7 cut(s) 40, 43, 56, 1036, 1160, 1307, 1962
BstNI CCWGG 6 cut(s) 339, 557, 1139, 1428, 1643, 1770
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 7 cut(s) 95, 337, 555, 1137, 1426, 1641, 1768
BstSFI CTRYAG 3 cut(s) 316, 622, 1529
BstSLI GKGCMC 1 cut(s) 1303
BstUI CGCG 1 cut(s) 933
BstV1I GCAGC 4 cut(s) 169, 608, 1543, 1996
BstV2I GAAGAC 2 cut(s) 399, 558
BstZI CGGCCG 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 591
BsuI GTATCC 2 cut(s) 254, 1228
BsuRI GGCC 7 cut(s) 95, 100, 110, 370, 856, 1526, 2102
BsuTUI ATCGAT 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 865
BtsCI GGATG 4 cut(s) 289, 895, 1437, 2025
BtsI GCAGTG 2 cut(s) 1905, 2076
BtsIMutI CAGTG 5 cut(s) 851, 1301, 1795, 1905, 2076
BtuMI TCGCGA 1 cut(s) 933
BveI ACCTGC 1 cut(s) 2070
Cac8I GCNNGC 3 cut(s) 48, 812, 1176
CaiI CAGNNNCTG 1 cut(s) 1931
CciI TCATGA 2 cut(s) 1020, 1270
Cfr13I GGNCC 5 cut(s) 98, 335, 368, 913, 1525
ClaI ATCGAT 1 cut(s) 591
Csp6I GTAC 7 cut(s) 329, 545, 583, 675, 939, 1008, 1411
CviAII CATG 8 cut(s) 332, 470, 811, 868, 1021, 1271, 1535, 1813
CviQI GTAC 7 cut(s) 329, 545, 583, 675, 939, 1008, 1411
DdeI CTNAG 4 cut(s) 10, 105, 117, 249
DpnI GATC 7 cut(s) 270, 636, 1170, 1593, 1843, 1984, 2050
DpnII GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
DraIII CACNNNGTG 3 cut(s) 360, 846, 1307
EaeI YGGCCR 2 cut(s) 93, 854
EagI CGGCCG 1 cut(s) 93
Eam1104I CTCTTC 2 cut(s) 879, 1574
EarI CTCTTC 2 cut(s) 879, 1574
EciI GGCGGA 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 11, 763
Eco47I GGWCC 2 cut(s) 335, 913
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 1598
Eco88I CYCGRG 1 cut(s) 774
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 913
EcoRII CCWGG 6 cut(s) 337, 555, 1137, 1426, 1641, 1768
EcoT38I GRGCYC 2 cut(s) 11, 763
FaeI CATG 8 cut(s) 335, 473, 814, 871, 1024, 1274, 1538, 1816
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 2 cut(s) 926, 1390
FatI CATG 8 cut(s) 331, 469, 810, 867, 1020, 1270, 1534, 1812
FauI CCCGC 1 cut(s) 1224
FauNDI CATATG 1 cut(s) 948
FbaI TGATCA 1 cut(s) 634
FblI GTMKAC 2 cut(s) 837, 1143
Fnu4HI GCNGC 8 cut(s) 35, 38, 41, 44, 183, 622, 1532, 2010
FokI GGATG 4 cut(s) 296, 902, 1444, 2032
FriOI GRGCYC 2 cut(s) 11, 763
Fsp4HI GCNGC 8 cut(s) 35, 38, 41, 44, 183, 622, 1532, 2010
FspBI CTAG 6 cut(s) 807, 987, 1080, 1506, 1515, 2034
GluI GCNGC 8 cut(s) 35, 38, 41, 44, 183, 622, 1532, 2010
GsaI CCCAGC 2 cut(s) 159, 1192
GsuI CTGGAG 4 cut(s) 1121, 1677, 1784, 1791
HaeIII GGCC 7 cut(s) 95, 100, 110, 370, 856, 1526, 2102
HapII CCGG 2 cut(s) 96, 1755
Hin1II CATG 8 cut(s) 335, 473, 814, 871, 1024, 1274, 1538, 1816
HincII GTYRAC 4 cut(s) 382, 785, 1144, 1948
HindII GTYRAC 4 cut(s) 382, 785, 1144, 1948
HindIII AAGCTT 2 cut(s) 140, 647
HinfI GANTC 7 cut(s) 834, 1196, 1216, 1646, 1733, 1928, 1934
HpaI GTTAAC 1 cut(s) 1948
HpaII CCGG 2 cut(s) 96, 1755
HphI GGTGA 6 cut(s) 16, 197, 1250, 1352, 1577, 1769
Hpy166II GTNNAC 9 cut(s) 382, 754, 785, 838, 1144, 1301, 1499, 1760, 1948
Hpy188I TCNGA 7 cut(s) 13, 120, 568, 686, 1554, 1933, 2053
Hpy188III TCNNGA 9 cut(s) 461, 822, 932, 1021, 1271, 1487, 1515, 1658, 1694
Hpy8I GTNNAC 9 cut(s) 382, 754, 785, 838, 1144, 1301, 1499, 1760, 1948
Hpy99I CGWCG 2 cut(s) 94, 1558
HpyAV CCTTC 5 cut(s) 1455, 1482, 1877, 1985, 2048
HpyCH4III ACNGT 6 cut(s) 386, 604, 846, 938, 1296, 1381
HpyCH4IV ACGT 2 cut(s) 1452, 1748
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 43, 56, 1036, 1160, 1307, 1962
HpyF3I CTNAG 4 cut(s) 10, 105, 117, 249
HpySE526I ACGT 2 cut(s) 1452, 1748
Hsp92II CATG 8 cut(s) 335, 473, 814, 871, 1024, 1274, 1538, 1816
KflI GGGWCCC 1 cut(s) 913
Ksp22I TGATCA 1 cut(s) 634
KspAI GTTAAC 1 cut(s) 1948
Kzo9I GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
LmnI GCTCC 2 cut(s) 1027, 1765
Lsp1109I GCAGC 4 cut(s) 169, 608, 1543, 1996
LweI GCATC 7 cut(s) 274, 454, 677, 1150, 1697, 1765, 1819
MaeI CTAG 6 cut(s) 807, 987, 1080, 1506, 1515, 2034
MaeII ACGT 2 cut(s) 1452, 1748
MaeIII GTNAC 8 cut(s) 73, 439, 730, 1248, 1256, 1795, 1804, 1901
MalI GATC 7 cut(s) 270, 636, 1170, 1593, 1843, 1984, 2050
MbiI CCGCTC 1 cut(s) 1231
MboI GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
MboII GAAGA 9 cut(s) 194, 391, 404, 446, 563, 896, 1591, 1597, 1838
MhlI GDGCHC 4 cut(s) 11, 763, 1093, 1303
MlsI TGGCCA 1 cut(s) 856
MluCI AATT 8 cut(s) 65, 210, 639, 954, 1414, 1437, 1662, 1783
MluNI TGGCCA 1 cut(s) 856
MlyI GAGTC 3 cut(s) 843, 1210, 1922
MmeI TCCRAC 2 cut(s) 114, 1577
Mox20I TGGCCA 1 cut(s) 856
MroXI GAANNNNTTC 1 cut(s) 1347
MscI TGGCCA 1 cut(s) 856
MseI TTAA 8 cut(s) 225, 489, 615, 645, 903, 1947, 2028, 2090
MslI CAYNNNNRTG 4 cut(s) 685, 1275, 1406, 1539
Msp20I TGGCCA 1 cut(s) 856
MspCI CTTAAG 1 cut(s) 644
MspI CCGG 2 cut(s) 96, 1755
MspR9I CCNGG 7 cut(s) 97, 339, 557, 1139, 1428, 1643, 1770
Mva1269I GAATGC 1 cut(s) 2011
MvaI CCWGG 6 cut(s) 339, 557, 1139, 1428, 1643, 1770
MvnI CGCG 1 cut(s) 933
MwoI GCNNNNNNNGC 7 cut(s) 40, 43, 56, 1036, 1160, 1307, 1962
NciI CCSGG 1 cut(s) 97
NdeI CATATG 1 cut(s) 948
NdeII GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
NlaIII CATG 8 cut(s) 335, 473, 814, 871, 1024, 1274, 1538, 1816
NlaIV GGNNCC 4 cut(s) 313, 914, 915, 1767
NmuCI GTSAC 3 cut(s) 73, 439, 1256
NruI TCGCGA 1 cut(s) 933
NspI RCATGY 1 cut(s) 814
NspV TTCGAA 1 cut(s) 607
PaeI GCATGC 1 cut(s) 814
PagI TCATGA 2 cut(s) 1020, 1270
PctI GAATGC 1 cut(s) 2011
PdmI GAANNNNTTC 1 cut(s) 1347
PfeI GAWTC 4 cut(s) 1196, 1646, 1733, 1934
PkrI GCNGC 8 cut(s) 36, 39, 42, 45, 184, 623, 1533, 2011
PleI GAGTC 3 cut(s) 842, 1210, 1922
PpsI GAGTC 3 cut(s) 842, 1210, 1922
PpuMI RGGWCCY 1 cut(s) 913
PsiI TTATAA 1 cut(s) 959
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 1748
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 6 cut(s) 337, 555, 1137, 1426, 1641, 1768
PspFI CCCAGC 2 cut(s) 155, 1188
PspGI CCWGG 6 cut(s) 337, 555, 1137, 1426, 1641, 1768
PspN4I GGNNCC 4 cut(s) 313, 914, 915, 1767
PspPI GGNCC 5 cut(s) 98, 335, 368, 913, 1525
PspPPI RGGWCCY 1 cut(s) 913
PsrI GAACNNNNNNTAC 2 cut(s) 974, 1006
PstI CTGCAG 2 cut(s) 626, 1533
PstNI CAGNNNCTG 1 cut(s) 1931
RruI TCGCGA 1 cut(s) 933
RsaI GTAC 7 cut(s) 330, 546, 584, 676, 940, 1009, 1412
RsaNI GTAC 7 cut(s) 329, 545, 583, 675, 939, 1008, 1411
RseI CAYNNNNRTG 4 cut(s) 685, 1275, 1406, 1539
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 1142
SaqAI TTAA 8 cut(s) 225, 489, 615, 645, 903, 1947, 2028, 2090
SatI GCNGC 8 cut(s) 35, 38, 41, 44, 183, 622, 1532, 2010
Sau3AI GATC 7 cut(s) 268, 634, 1168, 1591, 1841, 1982, 2048
Sau96I GGNCC 5 cut(s) 98, 335, 368, 913, 1525
SchI GAGTC 3 cut(s) 843, 1210, 1922
ScrFI CCNGG 7 cut(s) 97, 339, 557, 1139, 1428, 1643, 1770
SduI GDGCHC 4 cut(s) 11, 763, 1093, 1303
SfaNI GCATC 7 cut(s) 274, 454, 677, 1150, 1697, 1765, 1819
SfcI CTRYAG 3 cut(s) 316, 622, 1529
SfuI TTCGAA 1 cut(s) 607
SinI GGWCC 2 cut(s) 335, 913
SmiMI CAYNNNNRTG 4 cut(s) 685, 1275, 1406, 1539
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
SphI GCATGC 1 cut(s) 814
Sse9I AATT 8 cut(s) 65, 210, 639, 954, 1414, 1437, 1662, 1783
SsiI CCGC 7 cut(s) 32, 35, 38, 41, 44, 50, 1231
SspMI CTAG 6 cut(s) 807, 987, 1080, 1506, 1515, 2034
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 7 cut(s) 95, 337, 555, 1137, 1426, 1641, 1768
TaaI ACNGT 6 cut(s) 386, 604, 846, 938, 1296, 1381
TaiI ACGT 2 cut(s) 1455, 1751
TaqI TCGA 7 cut(s) 89, 510, 591, 607, 823, 1143, 1365
TasI AATT 8 cut(s) 65, 210, 639, 954, 1414, 1437, 1662, 1783
TatI WGTACW 3 cut(s) 582, 674, 1410
TauI GCSGC 4 cut(s) 37, 40, 43, 46
TfiI GAWTC 4 cut(s) 1196, 1646, 1733, 1934
Tru1I TTAA 8 cut(s) 225, 489, 615, 645, 903, 1947, 2028, 2090
Tru9I TTAA 8 cut(s) 225, 489, 615, 645, 903, 1947, 2028, 2090
TscAI CASTG 5 cut(s) 851, 1301, 1795, 1912, 2076
TseFI GTSAC 3 cut(s) 73, 439, 1256
TseI GCWGC 4 cut(s) 182, 621, 1531, 2009
Tsp45I GTSAC 3 cut(s) 73, 439, 1256
TspDTI ATGAA 4 cut(s) 497, 906, 995, 1557
TspGWI ACGGA 3 cut(s) 30, 1004, 1965
TspRI CASTG 5 cut(s) 851, 1301, 1795, 1912, 2076
Vha464I CTTAAG 1 cut(s) 644
VneI GTGCAC 1 cut(s) 1299
VpaK11BI GGWCC 2 cut(s) 335, 913
XapI RAATTY 2 cut(s) 639, 1437
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 1 cut(s) 814
XmiI GTMKAC 2 cut(s) 837, 1143
XmnI GAANNNNTTC 1 cut(s) 1347
XspI CTAG 6 cut(s) 807, 987, 1080, 1506, 1515, 2034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.