Rh6DG513200

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002762
Physical Location & Seq
Reverse (-)
66589868 .. 66590965
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG513200.1

Sequence Viewer

Length: 435 bp
ATGTCTGCGTACGATAACGTGGTTATTGGGAAGCTGAAGCTGAAAGGCAAAGCCTTGGACGTGAAAGGTGGGATCAAAAAGAAGAAGAAACGCAGCAAAACCTATGACTATGATCAAGCTCCTCCTCAAGCCTCAAAAGGTGGAGATGCAATAGATCAATTTGAGAATGATGGTGATGACGACATAGGGAAAGCTGGAGTATTTGATCAGATGACACCGGCAGAGAGGCGATATCTCGAGCAGGCAGAGAGGCTTCAAGTTCAAAGGCTTGCCAAAATGGCCAAGAAATCGCATCGTGATCGGGTTCAGGAATTCAATCAGTATCTGGCTAACCTATCTGAGCATTATGACATCCCCAAAGTAGGCCCTGAGACCACTGAATCCTACTGGACATTTTATCTTCATGCTTTGCAGCTGGATGCAATGCAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

144

Amino Acids

16.44

Weight (kDa)

8.63

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 3 - 86 4e-12 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 56
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 257, 263, 316
AjiI CACGTC 1 cut(s) 61
AluBI AGCT 5 cut(s) 34, 40, 119, 194, 415
AluI AGCT 5 cut(s) 34, 40, 119, 194, 415
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 2 cut(s) 279, 364
ApeKI GCWGC 2 cut(s) 93, 412
ApoI RAATTY 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 236
BalI TGGCCA 1 cut(s) 281
BbvI GCAGC 2 cut(s) 105, 424
BccI CCATC 1 cut(s) 164
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BclI TGATCA 2 cut(s) 112, 205
BcoDI GTCTC 1 cut(s) 365
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 2 cut(s) 94, 413
BlsI GCNGC 2 cut(s) 95, 414
BmeT110I CYCGRG 1 cut(s) 236
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 365
BmsI GCATC 3 cut(s) 136, 301, 409
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 2 cut(s) 351, 424
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 330, 360
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 2 cut(s) 111, 114
BseXI GCAGC 2 cut(s) 105, 424
BshFI GGCC 2 cut(s) 281, 366
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 1 cut(s) 218
BsiWI CGTACG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 2 cut(s) 281, 366
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 236
Bsp143I GATC 5 cut(s) 72, 112, 154, 205, 298
BspANI GGCC 2 cut(s) 281, 366
BspCNI CTCAG 2 cut(s) 331, 361
BspPI GGATC 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 5 cut(s) 72, 112, 154, 205, 298
BssT1I CCWWGG 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 243, 270
BstDEI CTNAG 2 cut(s) 339, 369
BstF5I GGATG 2 cut(s) 351, 424
BstKTI GATC 5 cut(s) 75, 115, 157, 208, 301
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 5 cut(s) 72, 112, 154, 205, 298
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 105, 424
BsuRI GGCC 2 cut(s) 281, 366
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 351, 424
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 2 cut(s) 243, 270
CaiI CAGNNNCTG 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 10
CviAII CATG 1 cut(s) 404
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 339, 369
DpnI GATC 5 cut(s) 74, 114, 156, 207, 300
DpnII GATC 5 cut(s) 72, 112, 154, 205, 298
EaeI YGGCCR 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 311
EcoRV GATATC 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 1 cut(s) 407
FaiI YATR 5 cut(s) 105, 111, 185, 348, 405
FatI CATG 1 cut(s) 403
FbaI TGATCA 2 cut(s) 112, 205
Fnu4HI GCNGC 2 cut(s) 94, 413
FokI GGATG 2 cut(s) 338, 431
Fsp4HI GCNGC 2 cut(s) 94, 413
GluI GCNGC 2 cut(s) 94, 413
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 281, 366
HapII CCGG 1 cut(s) 218
Hin1II CATG 1 cut(s) 407
HinfI GANTC 1 cut(s) 380
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 185
Hpy188I TCNGA 2 cut(s) 210, 340
Hpy188III TCNNGA 3 cut(s) 236, 296, 308
HpyCH4IV ACGT 2 cut(s) 18, 60
HpyCH4V TGCA 4 cut(s) 149, 412, 422, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 2 cut(s) 339, 369
HpySE526I ACGT 2 cut(s) 18, 60
Hsp92II CATG 1 cut(s) 407
Ksp22I TGATCA 2 cut(s) 112, 205
Kzo9I GATC 5 cut(s) 72, 112, 154, 205, 298
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 8 cut(s) 180, 227, 231, 293, 311, 373, 381, 401
Lsp1109I GCAGC 2 cut(s) 105, 424
LweI GCATC 3 cut(s) 136, 301, 409
MaeII ACGT 2 cut(s) 18, 60
MalI GATC 5 cut(s) 74, 114, 156, 207, 300
MboI GATC 5 cut(s) 72, 112, 154, 205, 298
MboII GAAGA 3 cut(s) 94, 97, 392
MfeI CAATTG 1 cut(s) 428
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 3 cut(s) 158, 311, 428
MluNI TGGCCA 1 cut(s) 281
MnlI CCTC 5 cut(s) 132, 135, 142, 219, 243
Mox20I TGGCCA 1 cut(s) 281
MscI TGGCCA 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeII GATC 5 cut(s) 72, 112, 154, 205, 298
NlaIII CATG 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 236
PfeI GAWTC 1 cut(s) 380
Pfl23II CGTACG 1 cut(s) 9
PkrI GCNGC 2 cut(s) 95, 414
PspLI CGTACG 1 cut(s) 9
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SatI GCNGC 2 cut(s) 94, 413
Sau3AI GATC 5 cut(s) 72, 112, 154, 205, 298
Sau96I GGNCC 1 cut(s) 365
SfaNI GCATC 3 cut(s) 136, 301, 409
Sfr274I CTCGAG 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 126, 236
SmoI CTYRAG 2 cut(s) 126, 236
Sse9I AATT 3 cut(s) 158, 311, 428
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 2 cut(s) 21, 63
TaqI TCGA 1 cut(s) 237
TasI AATT 3 cut(s) 158, 311, 428
TfiI GAWTC 1 cut(s) 380
TscAI CASTG 1 cut(s) 382
TseI GCWGC 2 cut(s) 93, 412
TspDTI ATGAA 1 cut(s) 392
TspRI CASTG 1 cut(s) 382
XapI RAATTY 1 cut(s) 311
XhoI CTCGAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.