Rorug02G0412300

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000653
Physical Location & Seq
Forward (+)
52824859 .. 52828221
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0412300.1

Sequence Viewer

Length: 1221 bp
ATGGGGAGAGCTAGAAGATGGTTGAAGGCTCTGTTGGGGATGAAGAAAGAGAAAGACCCTCTTGAGCTTCCAAATAGTACTACCAATACTACTCGGCCTGGTGATCGGAAAGAGAAGAGAAGGTGGAGTTTCGGCAAGTCATTGAAAGATGCAAGTCCAATTAGTCATGTCCCGGCTAATCTTCCCCCGAATGTTTCCCCTGCTGACTCAGCCTGGCTCAGATCCTATCTTGCCGAGACAGAGAAGGAACAGAACAAGCACGCAATTGCGGTGGCTGCTGCCACTGCCGCCGCTGCTGACGCTGCCGTGGCTGCTGCTCAGGCGGCGGTGGCGGTTGTGAGGCTGACGAGCAAGGGCTGTGGAGGAGACTTGTTTGGTAAGAGAGAGAGATGGGCGGCAATGAGGATTCAGACTGTTTTCAGAGGCTATTTGGCCAGGAAGGCGCACCGAGCTCTTAGAGGACTTGTTAAGTTGCAGGCTCTTGTTAGAGGCTTTCTTGTCAGGAAACGAGCTGCTGCTACTCTTCATGGTATGCAAGCTCTTATTAGAGCACAGACAAGTGTCCGATACCAGCGAGCACGTCGTTCTTTCAATAAAGAGAACAGGTTCCTACCCGAAAGCAGGCCTAGGAAGTCCATTGAGAGATTTGATGAAGCAAGAAGTGAATTTTATAGCAAGAGGCTTTCTGCAACTTATGAACCTTCTCTTAATGGCTTTGATGAAAGCCCAAAGATTGTTGAAATCGATACCTTCAGAACCAGATCCAAGTCTCGCCGATTCAACACCGTCTTATCTGAATATGGCGAAGATGTGCCTTACACCTCTTCGCCACTGCCATGTCCAATTCCAACTCGCATTACTGTACCTGGGGGCCAGCAGCTTCAGGATTATGAATGGTACTTCAATGCTGATGAATGCAAGTTCAGTACAGCCCATAGTACCCCCCGTTTGGCCAGCTCTTTTCGGTCTAATGCACCCAGTACACCTGCCAAGAGTGTTTGTGGAGACAGCTTCTTTTATCCTTACTCAAACTGCCCAAACTATATGTCAAGCACCAAGTCATTTAAGGCCAAATCAAGGTCCCACAGTGCACCGAAGCAAAGGCCTGAACCCCTGCCTAGGAAGAGGCTTTCCCTTGATGAAATAATGGCAGCAAGAAACAGTGTGAGTGGCATTCGAATGCATCGACCCTACAACCAAGTCCAAGAAGAAACAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
DUF1754 ERG4_ERG24

Protein Analysis

406

Amino Acids

45.65

Weight (kDa)

10.33

Isoelectric Point (pI)

62.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 309 - 390 2.2e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 994
Acc36I ACCTGC 1 cut(s) 994
AciI CCGC 7 cut(s) 269, 288, 291, 323, 326, 332, 395
AclWI GGATC 2 cut(s) 216, 756
AcoI YGGCCR 2 cut(s) 432, 951
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 2 cut(s) 736, 866
AfaI GTAC 6 cut(s) 79, 864, 899, 928, 940, 982
AfiI CCNNNNNNNGG 4 cut(s) 621, 949, 1077, 1119
AgsI TTSAA 6 cut(s) 25, 145, 592, 740, 781, 904
AjiI CACGTC 1 cut(s) 581
AjnI CCWGG 4 cut(s) 97, 212, 434, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 8 cut(s) 11, 67, 452, 512, 539, 880, 957, 1011
AluI AGCT 8 cut(s) 11, 67, 452, 512, 539, 880, 957, 1011
Alw21I GWGCWC 4 cut(s) 454, 553, 580, 1093
Alw26I GTCTC 4 cut(s) 230, 360, 774, 999
Alw44I GTGCAC 1 cut(s) 1089
AlwI GGATC 2 cut(s) 216, 756
AoxI GGCC 7 cut(s) 95, 432, 623, 871, 951, 1068, 1103
ApaLI GTGCAC 1 cut(s) 1089
ApoI RAATTY 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
Asp700I GAANNNNTTC 1 cut(s) 605
AspA2I CCTAGG 2 cut(s) 626, 1118
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 871, 1080
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 1177
AvaII GGWCC 1 cut(s) 1080
AvrII CCTAGG 2 cut(s) 626, 1118
BaeGI GKGCMC 1 cut(s) 1093
BalI TGGCCA 2 cut(s) 434, 953
BanII GRGCYC 1 cut(s) 454
Bbv12I GWGCWC 4 cut(s) 454, 553, 580, 1093
BccI CCATC 2 cut(s) 12, 384
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 4 cut(s) 99, 214, 436, 867
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 4 cut(s) 230, 360, 774, 999
BfaI CTAG 3 cut(s) 12, 627, 1119
BfuAI ACCTGC 1 cut(s) 994
BglI GCCNNNNNGGC 1 cut(s) 440
BlnI CCTAGG 2 cut(s) 626, 1118
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 5 cut(s) 99, 173, 214, 436, 867
Bme18I GGWCC 1 cut(s) 1080
BmgBI CACGTC 1 cut(s) 581
BmgT120I GGNCC 2 cut(s) 871, 1080
BmiI GGNNCC 3 cut(s) 608, 872, 1082
BmrFI CCNGG 5 cut(s) 99, 173, 214, 436, 867
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 2 cut(s) 139, 1192
BmuI ACTGGG 1 cut(s) 972
BoxI GACNNNNGTC 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 1177
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 744
BsaJI CCNNGG 4 cut(s) 306, 626, 866, 1118
Bsc4I CCNNNNNNNGG 4 cut(s) 621, 949, 1077, 1119
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 4 cut(s) 99, 214, 436, 867
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 4 cut(s) 306, 626, 866, 1118
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 4 cut(s) 621, 949, 1077, 1119
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 3 cut(s) 222, 232, 332
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 1093
BshFI GGCC 7 cut(s) 97, 434, 625, 873, 953, 1070, 1105
BshVI ATCGAT 1 cut(s) 744
BsiHKAI GWGCWC 4 cut(s) 454, 553, 580, 1093
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 2 cut(s) 155, 1066
BslI CCNNNNNNNGG 4 cut(s) 621, 949, 1077, 1119
BsmAI GTCTC 4 cut(s) 230, 360, 774, 999
BsmFI GGGAC 2 cut(s) 155, 1066
BsmI GAATGC 3 cut(s) 920, 1173, 1185
BsnI GGCC 7 cut(s) 97, 434, 625, 873, 953, 1070, 1105
Bsp119I TTCGAA 1 cut(s) 1177
Bsp1286I GDGCHC 4 cut(s) 454, 553, 580, 1093
Bsp143I GATC 3 cut(s) 103, 221, 761
BspACI CCGC 7 cut(s) 269, 288, 291, 323, 326, 332, 395
BspANI GGCC 7 cut(s) 97, 434, 625, 873, 953, 1070, 1105
BspCNI CTCAG 3 cut(s) 221, 231, 331
BspDI ATCGAT 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 608, 872, 1082
BspMI ACCTGC 1 cut(s) 994
BspPI GGATC 2 cut(s) 216, 756
BspT104I TTCGAA 1 cut(s) 1177
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 4 cut(s) 306, 626, 866, 1118
BssMI GATC 3 cut(s) 103, 221, 761
BssT1I CCWWGG 2 cut(s) 626, 1118
Bst2UI CCWGG 4 cut(s) 99, 214, 436, 867
Bst4CI ACNGT 6 cut(s) 415, 787, 862, 1088, 1163, 1216
Bst6I CTCTTC 4 cut(s) 110, 528, 829, 1118
BstBI TTCGAA 1 cut(s) 1177
BstC8I GCNNGC 7 cut(s) 261, 477, 537, 576, 623, 875, 955
BstDEI CTNAG 4 cut(s) 208, 218, 318, 455
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 106, 224, 764
BstMAI GTCTC 4 cut(s) 230, 360, 774, 999
BstMBI GATC 3 cut(s) 103, 221, 761
BstNI CCWGG 4 cut(s) 99, 214, 436, 867
BstPAI GACNNNNGTC 1 cut(s) 560
BstSCI CCNGG 5 cut(s) 97, 171, 212, 434, 865
BstSLI GKGCMC 1 cut(s) 1093
BstX2I RGATCY 2 cut(s) 221, 761
BstYI RGATCY 2 cut(s) 221, 761
Bsu15I ATCGAT 1 cut(s) 744
BsuRI GGCC 7 cut(s) 97, 434, 625, 873, 953, 1070, 1105
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 2 cut(s) 282, 830
BtsIMutI CAGTG 5 cut(s) 282, 830, 1093, 1168, 1221
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 7 cut(s) 261, 477, 537, 576, 623, 875, 955
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 871, 1080
ClaI ATCGAT 1 cut(s) 744
CseI GACGC 1 cut(s) 308
Csp6I GTAC 6 cut(s) 78, 863, 898, 927, 939, 981
CspCI CAANNNNNGTGG 4 cut(s) 252, 287, 340, 375
CviAII CATG 3 cut(s) 167, 527, 837
CviQI GTAC 6 cut(s) 78, 863, 898, 927, 939, 981
DdeI CTNAG 4 cut(s) 208, 218, 318, 455
DpnI GATC 3 cut(s) 105, 223, 763
DpnII GATC 3 cut(s) 103, 221, 761
EaeI YGGCCR 2 cut(s) 432, 951
Eam1104I CTCTTC 4 cut(s) 110, 528, 829, 1118
EarI CTCTTC 4 cut(s) 110, 528, 829, 1118
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 2 cut(s) 626, 1118
Eco147I AGGCCT 2 cut(s) 625, 1105
Eco24I GRGCYC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 1080
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 736, 866
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 1080
EcoRII CCWGG 4 cut(s) 97, 212, 434, 865
EcoT14I CCWWGG 2 cut(s) 626, 1118
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 626, 1118
FaeI CATG 3 cut(s) 170, 530, 840
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 155, 1066
FatI CATG 3 cut(s) 166, 526, 836
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 454
FspBI CTAG 3 cut(s) 12, 627, 1119
GlaI GCGC 1 cut(s) 444
HaeIII GGCC 7 cut(s) 97, 434, 625, 873, 953, 1070, 1105
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 170, 530, 840
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HinfI GANTC 3 cut(s) 206, 406, 777
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 2 cut(s) 983, 1091
Hpy188I TCNGA 7 cut(s) 108, 221, 411, 422, 566, 755, 796
Hpy188III TCNNGA 3 cut(s) 62, 502, 884
Hpy8I GTNNAC 2 cut(s) 983, 1091
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 6 cut(s) 19, 114, 238, 433, 711, 760
HpyCH4III ACNGT 6 cut(s) 415, 787, 862, 1088, 1163, 1216
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 8 cut(s) 152, 475, 535, 689, 918, 974, 1091, 1183
HpyF3I CTNAG 4 cut(s) 208, 218, 318, 455
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 3 cut(s) 170, 530, 840
HspAI GCGC 1 cut(s) 443
Kzo9I GATC 3 cut(s) 103, 221, 761
LweI GCATC 2 cut(s) 139, 1192
MaeI CTAG 3 cut(s) 12, 627, 1119
MaeII ACGT 1 cut(s) 580
MalI GATC 3 cut(s) 105, 223, 763
MboI GATC 3 cut(s) 103, 221, 761
MboII GAAGA 9 cut(s) 27, 55, 127, 173, 515, 816, 818, 1135, 1220
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 221, 761
MhlI GDGCHC 4 cut(s) 454, 553, 580, 1093
MlsI TGGCCA 2 cut(s) 434, 953
MluCI AATT 4 cut(s) 159, 264, 665, 843
MluNI TGGCCA 2 cut(s) 434, 953
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 872
Mox20I TGGCCA 2 cut(s) 434, 953
Mph1103I ATGCAT 1 cut(s) 1185
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 2 cut(s) 434, 953
MseI TTAA 3 cut(s) 468, 708, 1065
MslI CAYNNNNRTG 2 cut(s) 835, 1178
Msp20I TGGCCA 2 cut(s) 434, 953
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 5 cut(s) 99, 173, 214, 436, 867
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 3 cut(s) 920, 1173, 1185
MvaI CCWGG 4 cut(s) 99, 214, 436, 867
NciI CCSGG 1 cut(s) 173
NdeII GATC 3 cut(s) 103, 221, 761
NlaIII CATG 3 cut(s) 170, 530, 840
NlaIV GGNNCC 3 cut(s) 608, 872, 1082
NmeAIII GCCGAG 2 cut(s) 73, 259
NsiI ATGCAT 1 cut(s) 1185
NspV TTCGAA 1 cut(s) 1177
PaqCI CACCTGC 1 cut(s) 994
PceI AGGCCT 2 cut(s) 625, 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 1183
PctI GAATGC 3 cut(s) 920, 1173, 1185
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 406, 777
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1080
PshAI GACNNNNGTC 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 1080
Psp6I CCWGG 4 cut(s) 97, 212, 434, 865
PspGI CCWGG 4 cut(s) 97, 212, 434, 865
PspN4I GGNNCC 3 cut(s) 608, 872, 1082
PspPI GGNCC 2 cut(s) 871, 1080
PspPPI RGGWCCY 1 cut(s) 1080
PsuI RGATCY 2 cut(s) 221, 761
RsaI GTAC 6 cut(s) 79, 864, 899, 928, 940, 982
RsaNI GTAC 6 cut(s) 78, 863, 898, 927, 939, 981
RseI CAYNNNNRTG 2 cut(s) 835, 1178
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 468, 708, 1065
Sau3AI GATC 3 cut(s) 103, 221, 761
Sau96I GGNCC 2 cut(s) 871, 1080
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 5 cut(s) 99, 173, 214, 436, 867
SduI GDGCHC 4 cut(s) 454, 553, 580, 1093
SfaNI GCATC 2 cut(s) 139, 1192
SfuI TTCGAA 1 cut(s) 1177
SinI GGWCC 1 cut(s) 1080
SmiMI CAYNNNNRTG 2 cut(s) 835, 1178
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 159, 264, 665, 843
SseBI AGGCCT 2 cut(s) 625, 1105
SsiI CCGC 7 cut(s) 269, 288, 291, 323, 326, 332, 395
SspMI CTAG 3 cut(s) 12, 627, 1119
SstI GAGCTC 1 cut(s) 454
StuI AGGCCT 2 cut(s) 625, 1105
StyD4I CCNGG 5 cut(s) 97, 171, 212, 434, 865
StyI CCWWGG 2 cut(s) 626, 1118
TaaI ACNGT 6 cut(s) 415, 787, 862, 1088, 1163, 1216
TaiI ACGT 1 cut(s) 583
TaqI TCGA 3 cut(s) 744, 1177, 1186
TaqII GACCGA 1 cut(s) 954
TasI AATT 4 cut(s) 159, 264, 665, 843
TatI WGTACW 3 cut(s) 77, 926, 980
TauI GCSGC 4 cut(s) 290, 293, 326, 398
TfiI GAWTC 2 cut(s) 406, 777
Tru1I TTAA 3 cut(s) 468, 708, 1065
Tru9I TTAA 3 cut(s) 468, 708, 1065
TscAI CASTG 5 cut(s) 289, 837, 1093, 1168, 1221
TspDTI ATGAA 8 cut(s) 56, 515, 666, 711, 735, 906, 927, 1155
TspRI CASTG 5 cut(s) 289, 837, 1093, 1168, 1221
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 1 cut(s) 1080
XapI RAATTY 1 cut(s) 665
XmaJI CCTAGG 2 cut(s) 626, 1118
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 3 cut(s) 12, 627, 1119
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.