RLG00000020421

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000653
Physical Location & Seq
Forward (+)
64800443 .. 64802676
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020421

Sequence Viewer

Length: 615 bp
ATGGCCCCGACAAGGAGGAAGAGGACCAAGCAGAGGAGAAAGGCGAGGGCTGGTTTTACCAACAAGGAGGAGCAAAAACAAGTTGAGGCCTCGGAGCGTGAGATTGCGGATCTGAATCTGGGCGACAAGAGCAAGACTCATGGTACCAAGGGGCAAGAATCAGAGGGTTCTGGAGACATTAAAGGCGATGACTATTTGGGCGAGGATGTGTCAGGTAGCAAGGGGAAAAAATCAAAGGATCATCTAGGATTTGCCCGTGATGACTTTTTGGGCATAAATATTCCAGTGTCAAGTAGAAGGAAATTAGCGGGTTGTGCCTGTTGTGGCGAGTATGATCACCTTAATGTAACCTGCCCATTCCTAAAATATATTACTGAAGGCGCTAAAATTGGCTGTGGCTTTGAGACAATATGCCGGGGTTGTCACCATATTGGAAAGGTGTGCTGTGTTAAGAGGGGGAAGTCTGTGGGAGGATATGCTAAGCTGAAGAGTTGTGCTTATTGTTTCGGTATTGGTCACTGGGAGTGGGAGGACTGTGAAGATTGTCCTAGACCCAAGGACGTCGTCTTTGAGAAAGATGAATACTTGGATGGGGATGGGATTTCCTCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.53

Weight (kDa)

8.17

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 359
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 143
AciI CCGC 2 cut(s) 107, 308
AclWI GGATC 2 cut(s) 117, 246
AcuI CTGAAG 2 cut(s) 396, 506
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 2 cut(s) 12, 33
AluBI AGCT 1 cut(s) 484
AluI AGCT 1 cut(s) 484
Alw26I GTCTC 2 cut(s) 168, 398
AlwI GGATC 2 cut(s) 117, 246
AoxI GGCC 2 cut(s) 3, 87
ArsI GACNNNNNNTTYG 2 cut(s) 551, 583
Asp718I GGTACC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 4, 24
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 329, 416
AvaII GGWCC 1 cut(s) 24
BanI GGYRCC 1 cut(s) 143
BccI CCATC 2 cut(s) 584, 590
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 168, 398
BfaI CTAG 2 cut(s) 245, 549
BfoI RGCGCY 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 359
BlpI GCTNAGC 1 cut(s) 480
Bme1390I CCNGG 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 24
BmgT120I GGNCC 2 cut(s) 4, 24
BmiI GGNNCC 2 cut(s) 6, 145
BmrFI CCNGG 1 cut(s) 416
BmrI ACTGGG 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 529
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BpmI CTGGAG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 480
BpuMI CCSGG 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 90, 147, 415, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 33
Bse1I ACTGG 2 cut(s) 284, 524
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 4 cut(s) 90, 147, 415, 555
BseGI GGATG 3 cut(s) 211, 595, 601
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 12, 33
BseNI ACTGG 2 cut(s) 284, 524
BseRI GAGGAG 2 cut(s) 49, 83
BshFI GGCC 2 cut(s) 5, 89
BshNI GGYRCC 1 cut(s) 143
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 12, 33
BsmAI GTCTC 2 cut(s) 168, 398
BsnI GGCC 2 cut(s) 5, 89
Bsp143I GATC 3 cut(s) 109, 238, 334
Bsp1720I GCTNAGC 1 cut(s) 480
BspACI CCGC 2 cut(s) 107, 308
BspANI GGCC 2 cut(s) 5, 89
BspLI GGNNCC 2 cut(s) 6, 145
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 117, 246
BspT107I GGYRCC 1 cut(s) 143
BsrI ACTGG 2 cut(s) 284, 524
BssECI CCNNGG 4 cut(s) 90, 147, 415, 555
BssMI GATC 3 cut(s) 109, 238, 334
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 2 cut(s) 147, 555
Bst4CI ACNGT 1 cut(s) 536
Bst6I CTCTTC 2 cut(s) 14, 482
BstACI GRCGYC 1 cut(s) 561
BstDEI CTNAG 2 cut(s) 480, 607
BstF5I GGATG 3 cut(s) 211, 595, 601
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 3 cut(s) 112, 241, 337
BstMAI GTCTC 2 cut(s) 168, 398
BstMBI GATC 3 cut(s) 109, 238, 334
BstMWI GCNNNNNNNGC 2 cut(s) 129, 314
BstSCI CCNGG 1 cut(s) 414
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 2 cut(s) 5, 89
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 211, 595, 601
BtsIMutI CAGTG 2 cut(s) 291, 517
BveI ACCTGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 4, 24
Csp6I GTAC 1 cut(s) 144
CviAII CATG 1 cut(s) 140
CviJI RGCY 6 cut(s) 5, 50, 89, 393, 399, 484
CviKI_1 RGCY 6 cut(s) 5, 50, 89, 393, 399, 484
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 2 cut(s) 480, 607
DpnI GATC 3 cut(s) 111, 240, 336
DpnII GATC 3 cut(s) 109, 238, 334
Eam1104I CTCTTC 2 cut(s) 14, 482
EarI CTCTTC 2 cut(s) 14, 482
Eco130I CCWWGG 2 cut(s) 147, 555
Eco147I AGGCCT 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 396, 506
EcoT14I CCWWGG 2 cut(s) 147, 555
ErhI CCWWGG 2 cut(s) 147, 555
FaeI CATG 1 cut(s) 143
FaiI YATR 7 cut(s) 141, 275, 333, 369, 412, 429, 477
FatI CATG 1 cut(s) 139
FauI CCCGC 1 cut(s) 301
FbaI TGATCA 1 cut(s) 334
FokI GGATG 3 cut(s) 218, 602, 608
FspBI CTAG 2 cut(s) 245, 549
GlaI GCGC 1 cut(s) 382
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 5, 89
HapII CCGG 1 cut(s) 415
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 1 cut(s) 143
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 3 cut(s) 115, 136, 158
HpaII CCGG 1 cut(s) 415
HphI GGTGA 2 cut(s) 329, 416
Hpy188I TCNGA 4 cut(s) 94, 114, 163, 610
Hpy188III TCNNGA 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 2 cut(s) 291, 371
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4IV ACGT 1 cut(s) 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 314
HpyF3I CTNAG 2 cut(s) 480, 607
HpySE526I ACGT 1 cut(s) 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 1 cut(s) 143
HspAI GCGC 1 cut(s) 381
KpnI GGTACC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 109, 238, 334
LmnI GCTCC 2 cut(s) 70, 94
LpnPI CCDG 9 cut(s) 36, 104, 156, 198, 297, 331, 364, 428, 505
MaeI CTAG 2 cut(s) 245, 549
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 3 cut(s) 346, 422, 515
MalI GATC 3 cut(s) 111, 240, 336
MboI GATC 3 cut(s) 109, 238, 334
MboII GAAGA 3 cut(s) 31, 499, 551
MflI RGATCY 1 cut(s) 109
MluCI AATT 2 cut(s) 302, 387
MlyI GAGTC 1 cut(s) 130
MseI TTAA 3 cut(s) 180, 342, 450
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 129, 314
NciI CCSGG 1 cut(s) 416
NdeII GATC 3 cut(s) 109, 238, 334
NlaIII CATG 1 cut(s) 143
NlaIV GGNNCC 2 cut(s) 6, 145
NmuCI GTSAC 2 cut(s) 422, 515
PceI AGGCCT 1 cut(s) 89
PfeI GAWTC 2 cut(s) 115, 158
PflFI GACNNNGTC 1 cut(s) 563
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 6, 145
PspPI GGNCC 2 cut(s) 4, 24
PsuI RGATCY 1 cut(s) 109
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 3 cut(s) 180, 342, 450
Sau3AI GATC 3 cut(s) 109, 238, 334
Sau96I GGNCC 2 cut(s) 4, 24
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 416
SetI ASST 6 cut(s) 217, 342, 353, 441, 486, 564
SinI GGWCC 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 2 cut(s) 302, 387
SseBI AGGCCT 1 cut(s) 89
SsiI CCGC 2 cut(s) 107, 308
SspI AATATT 1 cut(s) 280
SspMI CTAG 2 cut(s) 245, 549
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 2 cut(s) 147, 555
TaaI ACNGT 1 cut(s) 536
TaiI ACGT 1 cut(s) 564
TasI AATT 2 cut(s) 302, 387
TfiI GAWTC 2 cut(s) 115, 158
Tru1I TTAA 3 cut(s) 180, 342, 450
Tru9I TTAA 3 cut(s) 180, 342, 450
TscAI CASTG 2 cut(s) 291, 524
TseFI GTSAC 2 cut(s) 422, 515
Tsp45I GTSAC 2 cut(s) 422, 515
TspDTI ATGAA 1 cut(s) 594
TspRI CASTG 2 cut(s) 291, 524
Tth111I GACNNNGTC 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 24
XspI CTAG 2 cut(s) 245, 549
ZraI GACGTC 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.