Rmu_sc0008712.1_g000008

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008712.1
OG0000653
Physical Location & Seq
Reverse (-)
23783 .. 31457
7675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008712.1_g000008.1.cds

Sequence Viewer

Length: 1059 bp
atgaaacgacataaacctaaaaagctcaaaataccggatctggaagacgacccgttgaagaagaggaaacctgttattgatcctgtatatgacgaagtttgcattggttgtggcataattggagagatctgctgttccaagggagcatatgctgttctcaagaggtgtggtgtttgtatggctatagccgcctataggtcactggtactgggacggcagaacaaggaagaggatgaggttatggtaaatgttgggaaggacaacaagaacggggatgaggttgtggtcgtggatgacaatggcaaggaagatgaggctaaagatcgagcagagcgagaaccactaggaagtgaggagaacttgctaaagcgtgactttagcttggctggggtgctagggcgttacccttgctcggctggttctgtaaccgttgtggtcgcggccctaccgtcggctcccgaggagactaggaccgaagttgagatctatgaggataacgataatgcatgcgagcaaggacgagtttgcggctctgctctcggatcgatgaccaatatgtgttcactgcagattgaaacgtacggatccataggagacatccagctgcacgcactagttgtttatgccaatgaggaacatgaagcactgctcaaacaggagcctcaagtggagagatggcttttcttggcatgtcacccttgcttgtggctgcgagttgaacgatccattaaagatccattggagcctggcgtcttccccagtattgagacagcggttgctgccgcaagtgcattggagttcctgtccacctgcaaatcggcaaacttaactgtaaaggtagagccgggttgttgcttgcttcatagaagtggaagactcacaatagatgggtgcgtgcttcagtgccagacaaatcccctagacttcctttcagtccccattgtgagtacggcaagtgcgcaaactctgttgtcttcaacggtgaagtgttataaggatggtgtttctgtttctcggactcaaattggaggtgccaactgccaaggctgtttcaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

352

Amino Acids

38.23

Weight (kDa)

5.14

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AarI CACCTGC 1 cut(s) 818
Acc16I TGCGCA 1 cut(s) 960
Acc36I ACCTGC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 1031
AccII CGCG 1 cut(s) 440
AciI CCGC 5 cut(s) 189, 440, 528, 773, 783
AclWI GGATC 7 cut(s) 45, 74, 550, 579, 592, 717, 728
AcuI CTGAAG 1 cut(s) 884
AcyI GRCGYC 1 cut(s) 750
AfaI GTAC 3 cut(s) 207, 581, 949
AfiI CCNNNNNNNGG 3 cut(s) 195, 412, 451
AgsI TTSAA 5 cut(s) 58, 575, 719, 978, 1054
AhlI ACTAGT 1 cut(s) 613
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 3 cut(s) 25, 381, 604
AluI AGCT 3 cut(s) 25, 381, 604
Alw26I GTCTC 3 cut(s) 458, 588, 761
AlwI GGATC 7 cut(s) 45, 74, 550, 579, 592, 717, 728
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 3 cut(s) 604, 709, 779
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 2 cut(s) 442, 471
AsuC2I CCSGG 1 cut(s) 846
AsuHPI GGTGA 2 cut(s) 686, 994
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 471
BamHI GGATCC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 1031
BbsI GAAGAC 4 cut(s) 51, 745, 880, 966
BbvI GCAGC 3 cut(s) 591, 696, 766
BccI CCATC 3 cut(s) 669, 881, 992
BceAI ACGGC 2 cut(s) 230, 966
BciT130I CCWGG 1 cut(s) 747
BcnI CCSGG 1 cut(s) 846
BcoDI GTCTC 3 cut(s) 458, 588, 761
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 6 cut(s) 344, 395, 468, 614, 920, 1057
BfmI CTRYAG 3 cut(s) 183, 193, 566
BfuAI ACCTGC 1 cut(s) 818
BglII AGATCT 2 cut(s) 126, 483
BisI GCNGC 7 cut(s) 189, 441, 529, 605, 710, 780, 783
BlsI GCNGC 7 cut(s) 190, 442, 530, 606, 711, 781, 784
Bme1390I CCNGG 2 cut(s) 747, 846
Bme18I GGWCC 1 cut(s) 471
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 442, 471
BmiI GGNNCC 5 cut(s) 456, 586, 660, 744, 1033
BmrFI CCNGG 2 cut(s) 747, 846
BmrI ACTGGG 2 cut(s) 218, 753
BmuI ACTGGG 2 cut(s) 218, 753
BpiI GAAGAC 4 cut(s) 51, 745, 880, 966
BpuEI CTTGAG 2 cut(s) 143, 648
BpuMI CCSGG 1 cut(s) 846
Bsa29I ATCGAT 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 750
BsaJI CCNNGG 3 cut(s) 138, 459, 1042
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 412, 451
Bse1I ACTGG 3 cut(s) 207, 213, 759
BseBI CCWGG 1 cut(s) 747
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 3 cut(s) 138, 459, 1042
BseGI GGATG 5 cut(s) 238, 280, 298, 597, 1003
BseLI CCNNNNNNNGG 3 cut(s) 195, 412, 451
BseNI ACTGG 3 cut(s) 207, 213, 759
BseRI GAGGAG 2 cut(s) 368, 476
BseXI GCAGC 3 cut(s) 591, 696, 766
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 443
BshNI GGYRCC 1 cut(s) 1031
BshVI ATCGAT 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 35, 845
BsiWI CGTACG 1 cut(s) 579
BslFI GGGAC 2 cut(s) 225, 920
BslI CCNNNNNNNGG 3 cut(s) 195, 412, 451
BsmAI GTCTC 3 cut(s) 458, 588, 761
BsmFI GGGAC 2 cut(s) 225, 920
BsnI GGCC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 458
Bsp143I GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
BspACI CCGC 5 cut(s) 189, 440, 528, 773, 783
BspANI GGCC 1 cut(s) 443
BspDI ATCGAT 1 cut(s) 545
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 5 cut(s) 456, 586, 660, 744, 1033
BspMAI CTGCAG 1 cut(s) 570
BspMI ACCTGC 1 cut(s) 818
BspPI GGATC 7 cut(s) 45, 74, 550, 579, 592, 717, 728
BspT107I GGYRCC 1 cut(s) 1031
BsrI ACTGG 3 cut(s) 207, 213, 759
BssECI CCNNGG 3 cut(s) 138, 459, 1042
BssMI GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
BssNI GRCGYC 1 cut(s) 750
BssT1I CCWWGG 2 cut(s) 138, 1042
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 4 cut(s) 430, 450, 832, 982
Bst6I CTCTTC 2 cut(s) 56, 222
BstACI GRCGYC 1 cut(s) 750
BstC8I GCNNGC 5 cut(s) 508, 512, 609, 857, 896
BstF5I GGATG 5 cut(s) 238, 280, 298, 597, 1003
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 9 cut(s) 40, 82, 129, 325, 486, 545, 587, 725, 736
BstMAI GTCTC 3 cut(s) 458, 588, 761
BstMBI GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
BstMWI GCNNNNNNNGC 3 cut(s) 188, 779, 788
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 2 cut(s) 510, 693
BstSCI CCNGG 2 cut(s) 745, 844
BstSFI CTRYAG 3 cut(s) 183, 193, 566
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 591, 696, 766
BstV2I GAAGAC 4 cut(s) 51, 745, 880, 966
BstX2I RGATCY 5 cut(s) 37, 126, 483, 584, 733
BstYI RGATCY 5 cut(s) 37, 126, 483, 584, 733
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 1 cut(s) 443
BsuTUI ATCGAT 1 cut(s) 545
BtsCI GGATG 5 cut(s) 238, 280, 298, 597, 1003
BtsI GCAGTG 2 cut(s) 563, 644
BtsIMutI CAGTG 4 cut(s) 200, 563, 644, 908
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 5 cut(s) 508, 512, 609, 857, 896
CfoI GCGC 1 cut(s) 961
Cfr13I GGNCC 2 cut(s) 442, 471
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 1 cut(s) 739
Csp6I GTAC 3 cut(s) 206, 580, 948
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 3 cut(s) 507, 638, 690
CviQI GTAC 3 cut(s) 206, 580, 948
DpnI GATC 9 cut(s) 39, 81, 128, 324, 485, 544, 586, 724, 735
DpnII GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
Eam1104I CTCTTC 2 cut(s) 56, 222
EarI CTCTTC 2 cut(s) 56, 222
Eco130I CCWWGG 2 cut(s) 138, 1042
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 1 cut(s) 884
Eco88I CYCGRG 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 138, 1042
EcoT22I ATGCAT 1 cut(s) 508
ErhI CCWWGG 2 cut(s) 138, 1042
FaeI CATG 3 cut(s) 510, 641, 693
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 2 cut(s) 225, 920
FatI CATG 3 cut(s) 506, 637, 689
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 7 cut(s) 189, 441, 529, 605, 710, 780, 783
FokI GGATG 5 cut(s) 245, 287, 305, 584, 1010
Fsp4HI GCNGC 7 cut(s) 189, 441, 529, 605, 710, 780, 783
FspBI CTAG 6 cut(s) 344, 395, 468, 614, 920, 1057
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 1 cut(s) 960
GluI GCNGC 7 cut(s) 189, 441, 529, 605, 710, 780, 783
GsaI CCCAGC 1 cut(s) 390
HaeIII GGCC 1 cut(s) 443
HapII CCGG 2 cut(s) 35, 845
HgaI GACGC 1 cut(s) 739
HhaI GCGC 1 cut(s) 961
Hin1I GRCGYC 1 cut(s) 750
Hin1II CATG 3 cut(s) 510, 641, 693
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HinfI GANTC 2 cut(s) 876, 1018
HpaII CCGG 2 cut(s) 35, 845
HphI GGTGA 2 cut(s) 686, 994
Hpy166II GTNNAC 2 cut(s) 563, 807
Hpy188I TCNGA 2 cut(s) 542, 1017
Hpy188III TCNNGA 3 cut(s) 41, 160, 458
Hpy8I GTNNAC 2 cut(s) 563, 807
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 4 cut(s) 430, 450, 832, 982
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 6 cut(s) 102, 506, 568, 607, 791, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 779, 788
HpySE526I ACGT 1 cut(s) 578
Hsp92I GRCGYC 1 cut(s) 750
Hsp92II CATG 3 cut(s) 510, 641, 693
HspAI GCGC 1 cut(s) 959
Kzo9I GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
LmnI GCTCC 4 cut(s) 143, 460, 658, 742
Lsp1109I GCAGC 3 cut(s) 591, 696, 766
MaeI CTAG 6 cut(s) 344, 395, 468, 614, 920, 1057
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 5 cut(s) 198, 371, 401, 424, 692
MalI GATC 9 cut(s) 39, 81, 128, 324, 485, 544, 586, 724, 735
MboI GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
MboII GAAGA 8 cut(s) 56, 70, 73, 239, 320, 745, 885, 966
MflI RGATCY 5 cut(s) 37, 126, 483, 584, 733
MluCI AATT 2 cut(s) 117, 1023
MlyI GAGTC 2 cut(s) 870, 1012
Mph1103I ATGCAT 1 cut(s) 508
MseI TTAA 2 cut(s) 729, 827
MslI CAYNNNNRTG 1 cut(s) 867
MspA1I CMGCKG 2 cut(s) 604, 773
MspI CCGG 2 cut(s) 35, 845
MspR9I CCNGG 2 cut(s) 747, 846
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 188, 779, 788
NciI CCSGG 1 cut(s) 846
NdeI CATATG 1 cut(s) 148
NdeII GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
NlaIII CATG 3 cut(s) 510, 641, 693
NlaIV GGNNCC 5 cut(s) 456, 586, 660, 744, 1033
NmeAIII GCCGAG 1 cut(s) 392
NmuCI GTSAC 3 cut(s) 198, 371, 692
NsbI TGCGCA 1 cut(s) 960
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 2 cut(s) 510, 693
PaeI GCATGC 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 331
Pfl23II CGTACG 1 cut(s) 579
PkrI GCNGC 7 cut(s) 190, 442, 530, 606, 711, 781, 784
PleI GAGTC 2 cut(s) 870, 1012
PpsI GAGTC 2 cut(s) 870, 1012
PsiI TTATAA 1 cut(s) 993
Psp6I CCWGG 1 cut(s) 745
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 745
PspLI CGTACG 1 cut(s) 579
PspN4I GGNNCC 5 cut(s) 456, 586, 660, 744, 1033
PspPI GGNCC 2 cut(s) 442, 471
PstI CTGCAG 1 cut(s) 570
PsuI RGATCY 5 cut(s) 37, 126, 483, 584, 733
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 3 cut(s) 207, 581, 949
RsaNI GTAC 3 cut(s) 206, 580, 948
RseI CAYNNNNRTG 1 cut(s) 867
SaqAI TTAA 2 cut(s) 729, 827
SatI GCNGC 7 cut(s) 189, 441, 529, 605, 710, 780, 783
Sau3AI GATC 9 cut(s) 37, 79, 126, 322, 483, 542, 584, 722, 733
Sau96I GGNCC 2 cut(s) 442, 471
SchI GAGTC 2 cut(s) 870, 1012
ScrFI CCNGG 2 cut(s) 747, 846
SfcI CTRYAG 3 cut(s) 183, 193, 566
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 2 cut(s) 158, 663
SmoI CTYRAG 2 cut(s) 158, 663
SpeI ACTAGT 1 cut(s) 613
SphI GCATGC 1 cut(s) 510
Sse9I AATT 2 cut(s) 117, 1023
SsiI CCGC 5 cut(s) 189, 440, 528, 773, 783
SspMI CTAG 6 cut(s) 344, 395, 468, 614, 920, 1057
StyD4I CCNGG 2 cut(s) 745, 844
StyI CCWWGG 2 cut(s) 138, 1042
TaaI ACNGT 4 cut(s) 430, 450, 832, 982
TaiI ACGT 1 cut(s) 581
TaqI TCGA 2 cut(s) 325, 545
TaqII GACCGA 1 cut(s) 488
TasI AATT 2 cut(s) 117, 1023
TauI GCSGC 4 cut(s) 191, 443, 531, 785
Tru1I TTAA 2 cut(s) 729, 827
Tru9I TTAA 2 cut(s) 729, 827
TscAI CASTG 4 cut(s) 207, 570, 651, 908
TseFI GTSAC 3 cut(s) 198, 371, 692
TseI GCWGC 3 cut(s) 604, 709, 779
Tsp45I GTSAC 3 cut(s) 198, 371, 692
TspDTI ATGAA 3 cut(s) 17, 654, 851
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 4 cut(s) 207, 570, 651, 908
VpaK11BI GGWCC 1 cut(s) 471
XceI RCATGY 2 cut(s) 510, 693
XspI CTAG 6 cut(s) 344, 395, 468, 614, 920, 1057
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.