RLG00000020408

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000653
Physical Location & Seq
Reverse (-)
64719778 .. 64723830
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020408

Sequence Viewer

Length: 666 bp
ATGTCTACCGCGTCCGCCGTCTCGCCCACAACCTTAGCATTGCTCTCAAGGAGGTCCACGACTACCAGTCGGCCTTCCGCGAAATCGAGGCCCTCCAGACACTCCACGGCTGCCTCAACGTCGTCGTTTTGTACTAGTACTTCCGGCTCGACGAGGAGGACGCCGTGCTCGTGCTCGAGTTTCTGGCCAGTGACCTGGAGACTGATCAAAGCGGCGAAGAAGGAGGGCGGGATTGGCTGCAGCGAGGTCAAAAGTGTAACCTCAGGTACCAAGGGGCAAGAATCAGAGAGTTCTAGAGACATTAAAGGTGATGACTATTTGGGCGAGGATGTGTCAGTGTCATGTAGAAGCAAATTAGCGGGTTGTTCCTGTTGTGGCAAGTATGATCACCTTAATTTAACCTACCCATTCCTAAAATATTTTCCTGAAGGCACTAAAATTGGCCATGGCTTTGAGACAATGTGCCGGGGTTGTCACCATATTGGAAAGGTGTGCTGTGTTAAGAGAGGGAAGTTTGTGGGAGGATATGCAATGCTGAAGAGGTGTACTTATTGTTACGGTATTGGTCATTGGGAGTGGGAGGACTGTAAAGATTGTCCTAGACCTAAGGACGTCGTCTATGAGAAACCTGAATACTTAGATGGGGATGGGATTTCCTCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.07

Weight (kDa)

8.97

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 615
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 11, 80
AciI CCGC 6 cut(s) 9, 15, 78, 212, 228, 359
AcoI YGGCCR 2 cut(s) 185, 442
AcuI CTGAAG 2 cut(s) 447, 557
AcyI GRCGYC 2 cut(s) 161, 612
AfaI GTAC 4 cut(s) 133, 139, 268, 547
AhdI GACNNNNNGTC 1 cut(s) 66
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 194
Alw21I GWGCWC 2 cut(s) 170, 176
Alw26I GTCTC 4 cut(s) 25, 193, 291, 449
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 4 cut(s) 71, 89, 185, 442
ApeKI GCWGC 3 cut(s) 110, 237, 240
Asp718I GGTACC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 54, 90
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 3 cut(s) 320, 380, 467
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 1 cut(s) 54
AxyI CCTNAGG 2 cut(s) 262, 606
BalI TGGCCA 2 cut(s) 187, 444
BanI GGYRCC 1 cut(s) 266
BarI GAAGNNNNNNTAC 2 cut(s) 124, 156
BauI CACGAG 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 170, 176
BbvI GCAGC 3 cut(s) 97, 224, 252
BccI CCATC 2 cut(s) 635, 641
BceAI ACGGC 3 cut(s) 2, 123, 148
BciT130I CCWGG 1 cut(s) 196
BclI TGATCA 2 cut(s) 204, 385
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 4 cut(s) 25, 193, 291, 449
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 3 cut(s) 135, 294, 600
BfmI CTRYAG 1 cut(s) 238
BisI GCNGC 4 cut(s) 111, 213, 238, 241
BlsI GCNGC 4 cut(s) 112, 214, 239, 242
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 2 cut(s) 196, 467
Bme18I GGWCC 1 cut(s) 54
BmeRI GACNNNNNGTC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 54, 90
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 196, 467
BpmI CTGGAG 2 cut(s) 79, 217
Bpu10I CCTNAGC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 467
BsaHI GRCGYC 2 cut(s) 161, 612
BsaJI CCNNGG 4 cut(s) 105, 270, 445, 466
Bse1I ACTGG 2 cut(s) 66, 188
Bse21I CCTNAGG 2 cut(s) 262, 606
Bse3DI GCAATG 2 cut(s) 38, 537
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 4 cut(s) 105, 270, 445, 466
BseGI GGATG 2 cut(s) 334, 652
BseMI GCAATG 2 cut(s) 38, 537
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 2 cut(s) 66, 188
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 97, 224, 252
Bsh1236I CGCG 2 cut(s) 11, 80
BshFI GGCC 4 cut(s) 73, 91, 187, 444
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 2 cut(s) 170, 176
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 2 cut(s) 144, 466
BsmAI GTCTC 4 cut(s) 25, 193, 291, 449
BsmBI CGTCTC 1 cut(s) 25
BsnI GGCC 4 cut(s) 73, 91, 187, 444
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 170, 176
Bsp143I GATC 2 cut(s) 204, 385
Bsp19I CCATGG 1 cut(s) 445
BspACI CCGC 6 cut(s) 9, 15, 78, 212, 228, 359
BspANI GGCC 4 cut(s) 73, 91, 187, 444
BspCNI CTCAG 1 cut(s) 275
BspFNI CGCG 2 cut(s) 11, 80
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 38, 537
BsrI ACTGG 2 cut(s) 66, 188
BssECI CCNNGG 4 cut(s) 105, 270, 445, 466
BssMI GATC 2 cut(s) 204, 385
BssNI GRCGYC 2 cut(s) 161, 612
BssSI CACGAG 1 cut(s) 169
BssT1I CCWWGG 2 cut(s) 270, 445
Bst2BI CACGAG 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 2 cut(s) 560, 587
Bst6I CTCTTC 1 cut(s) 533
BstACI GRCGYC 2 cut(s) 161, 612
BstDEI CTNAG 5 cut(s) 34, 262, 606, 637, 658
BstDSI CCRYGG 2 cut(s) 105, 445
BstF5I GGATG 2 cut(s) 334, 652
BstFNI CGCG 2 cut(s) 11, 80
BstKTI GATC 2 cut(s) 207, 388
BstMAI GTCTC 4 cut(s) 25, 193, 291, 449
BstMBI GATC 2 cut(s) 204, 385
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 465
BstSFI CTRYAG 1 cut(s) 238
BstUI CGCG 2 cut(s) 11, 80
BstV1I GCAGC 3 cut(s) 97, 224, 252
BstXI CCANNNNNNTGG 1 cut(s) 195
Bsu36I CCTNAGG 2 cut(s) 262, 606
BsuRI GGCC 4 cut(s) 73, 91, 187, 444
BtgI CCRYGG 2 cut(s) 105, 445
BtsCI GGATG 2 cut(s) 334, 652
BtsIMutI CAGTG 2 cut(s) 195, 342
Cfr13I GGNCC 2 cut(s) 54, 90
CseI GACGC 1 cut(s) 169
Csp6I GTAC 4 cut(s) 132, 138, 267, 546
CviAII CATG 2 cut(s) 342, 446
CviJI RGCY 8 cut(s) 73, 91, 110, 147, 187, 237, 444, 450
CviKI_1 RGCY 8 cut(s) 73, 91, 110, 147, 187, 237, 444, 450
CviQI GTAC 4 cut(s) 132, 138, 267, 546
DdeI CTNAG 5 cut(s) 34, 262, 606, 637, 658
DpnI GATC 2 cut(s) 206, 387
DpnII GATC 2 cut(s) 204, 385
DriI GACNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 2 cut(s) 185, 442
Eam1104I CTCTTC 1 cut(s) 533
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 533
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 2 cut(s) 270, 445
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 2 cut(s) 447, 557
Eco81I CCTNAGG 2 cut(s) 262, 606
Eco88I CYCGRG 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 2 cut(s) 270, 445
ErhI CCWWGG 2 cut(s) 270, 445
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 2 cut(s) 345, 449
FaiI YATR 6 cut(s) 343, 384, 447, 480, 528, 621
FatI CATG 2 cut(s) 341, 445
FauI CCCGC 2 cut(s) 221, 352
FbaI TGATCA 2 cut(s) 204, 385
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 111, 213, 238, 241
FokI GGATG 2 cut(s) 341, 659
Fsp4HI GCNGC 4 cut(s) 111, 213, 238, 241
FspBI CTAG 3 cut(s) 135, 294, 600
GluI GCNGC 4 cut(s) 111, 213, 238, 241
GsuI CTGGAG 2 cut(s) 79, 217
HaeIII GGCC 4 cut(s) 73, 91, 187, 444
HapII CCGG 2 cut(s) 144, 466
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 2 cut(s) 161, 612
Hin1II CATG 2 cut(s) 345, 449
HinfI GANTC 1 cut(s) 281
HpaII CCGG 2 cut(s) 144, 466
HphI GGTGA 3 cut(s) 320, 380, 467
Hpy166II GTNNAC 3 cut(s) 6, 57, 546
Hpy188I TCNGA 2 cut(s) 286, 665
Hpy188III TCNNGA 3 cut(s) 96, 294, 425
Hpy8I GTNNAC 3 cut(s) 6, 57, 546
Hpy99I CGWCG 4 cut(s) 124, 127, 154, 617
HpyAV CCTTC 3 cut(s) 84, 214, 422
HpyCH4III ACNGT 2 cut(s) 560, 587
HpyCH4IV ACGT 2 cut(s) 119, 612
HpyCH4V TGCA 2 cut(s) 240, 530
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 5 cut(s) 34, 262, 606, 637, 658
HpySE526I ACGT 2 cut(s) 119, 612
Hsp92I GRCGYC 2 cut(s) 161, 612
Hsp92II CATG 2 cut(s) 345, 449
KpnI GGTACC 1 cut(s) 270
Ksp22I TGATCA 2 cut(s) 204, 385
Kzo9I GATC 2 cut(s) 204, 385
Lsp1109I GCAGC 3 cut(s) 97, 224, 252
MaeI CTAG 3 cut(s) 135, 294, 600
MaeII ACGT 2 cut(s) 119, 612
MaeIII GTNAC 4 cut(s) 190, 256, 473, 554
MalI GATC 2 cut(s) 206, 387
MboI GATC 2 cut(s) 204, 385
MboII GAAGA 2 cut(s) 229, 550
MhlI GDGCHC 2 cut(s) 170, 176
MlsI TGGCCA 2 cut(s) 187, 444
MluCI AATT 3 cut(s) 353, 394, 438
MluNI TGGCCA 2 cut(s) 187, 444
Mox20I TGGCCA 2 cut(s) 187, 444
MscI TGGCCA 2 cut(s) 187, 444
MseI TTAA 4 cut(s) 303, 393, 398, 501
Msp20I TGGCCA 2 cut(s) 187, 444
MspI CCGG 2 cut(s) 144, 466
MspR9I CCNGG 2 cut(s) 196, 467
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 2 cut(s) 11, 80
MwoI GCNNNNNNNGC 1 cut(s) 234
NciI CCSGG 1 cut(s) 467
NcoI CCATGG 1 cut(s) 445
NdeII GATC 2 cut(s) 204, 385
NlaIII CATG 2 cut(s) 345, 449
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 190, 473
PaeR7I CTCGAG 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 1 cut(s) 281
PflFI GACNNNGTC 1 cut(s) 614
PkrI GCNGC 4 cut(s) 112, 214, 239, 242
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 2 cut(s) 54, 90
PspXI VCTCGAGB 1 cut(s) 175
PstI CTGCAG 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 614
RsaI GTAC 4 cut(s) 133, 139, 268, 547
RsaNI GTAC 4 cut(s) 132, 138, 267, 546
SaqAI TTAA 4 cut(s) 303, 393, 398, 501
SatI GCNGC 4 cut(s) 111, 213, 238, 241
Sau3AI GATC 2 cut(s) 204, 385
Sau96I GGNCC 2 cut(s) 54, 90
ScaI AGTACT 1 cut(s) 139
ScrFI CCNGG 2 cut(s) 196, 467
SduI GDGCHC 2 cut(s) 170, 176
SfcI CTRYAG 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 175
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 46, 175
SmoI CTYRAG 2 cut(s) 46, 175
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 3 cut(s) 353, 394, 438
SsiI CCGC 6 cut(s) 9, 15, 78, 212, 228, 359
SspI AATATT 1 cut(s) 419
SspMI CTAG 3 cut(s) 135, 294, 600
StyD4I CCNGG 2 cut(s) 194, 465
StyI CCWWGG 2 cut(s) 270, 445
TaaI ACNGT 2 cut(s) 560, 587
TaiI ACGT 2 cut(s) 122, 615
TaqI TCGA 3 cut(s) 86, 149, 176
TasI AATT 3 cut(s) 353, 394, 438
TatI WGTACW 3 cut(s) 131, 137, 545
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 4 cut(s) 303, 393, 398, 501
Tru9I TTAA 4 cut(s) 303, 393, 398, 501
TscAI CASTG 2 cut(s) 195, 342
TseFI GTSAC 2 cut(s) 190, 473
TseI GCWGC 3 cut(s) 110, 237, 240
Tsp45I GTSAC 2 cut(s) 190, 473
TspRI CASTG 2 cut(s) 195, 342
Tth111I GACNNNGTC 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 54
XbaI TCTAGA 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 3 cut(s) 135, 294, 600
ZraI GACGTC 1 cut(s) 613
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.