Rh2DG494000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000653
Physical Location & Seq
Forward (+)
71414662 .. 71420745
6084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG494000.1

Sequence Viewer

Length: 582 bp
ATGCCCCGGCCGAAAAACCGAAATCTATCTATGACAAAGAAGGTAGCAAGGGGCAAGAATCAGAGGGTTCTGGAGACATTAAAGGTGATGACTATTTGGGCGAGGATGTGTCAGTGTAACCTCAAAACAGGTAGCGAGGGGAAAGAATCAAAGGATCATCTAAGAACTGCCCGTGATGACTTTTTGGGCATAGATATTCCAGTGTCATGTAGAAGGAAATTAGCGGGTTGTGCCTGTTGTGGCGAGTATGATCACCTTAATGTAACCTGCCCATTCCTAAAATATATTCCTGATGGCGCTAAAATTGGCCGTTGCTTTGAGACAATATGTCGGGGTTGTCACCATATTGGAAAGGTGTGCTGTGTTAAGAGGGGGAAGTCTGTGGGAGGATATGCTATGCTGAAGAGGTGTTGTCATTGTTTATGTATTGGTCACTGGGAGTGGGAGGACTGTGAAGATTGTCCTAGACCCAAGGACGTCGTCTATGAGAAACTTGAATACTTAGATGGGGATGGGATTTCCTCAGAGTCTGAGGATGAGGATGCATTTCGACCCCGTAATCGTGCTCCATGGGGACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.93

Weight (kDa)

8.7

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 275
AciI CCGC 1 cut(s) 224
AclWI GGATC 1 cut(s) 162
AcoI YGGCCR 2 cut(s) 8, 307
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 477
AgsI TTSAA 1 cut(s) 497
AhdI GACNNNNNGTC 1 cut(s) 327
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 68, 314
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 2 cut(s) 8, 307
AspLEI GCGC 1 cut(s) 299
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 3 cut(s) 97, 245, 332
Bbv12I GWGCWC 1 cut(s) 568
BccI CCATC 3 cut(s) 287, 500, 506
BceAI ACGGC 1 cut(s) 294
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 2 cut(s) 68, 314
BfaI CTAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 7
BmeRI GACNNNNNGTC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 477
BsaJI CCNNGG 3 cut(s) 5, 471, 569
Bse1I ACTGG 2 cut(s) 200, 440
BseDI CCNNGG 3 cut(s) 5, 471, 569
BseGI GGATG 4 cut(s) 111, 517, 541, 547
BseMII CTCAG 2 cut(s) 522, 537
BseNI ACTGG 2 cut(s) 200, 440
BseX3I CGGCCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 10, 309
BsiEI CGRYCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 1 cut(s) 7
BsmAI GTCTC 2 cut(s) 68, 314
BsnI GGCC 2 cut(s) 10, 309
Bsp1286I GDGCHC 1 cut(s) 568
Bsp143I GATC 2 cut(s) 154, 250
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 1 cut(s) 224
BspANI GGCC 2 cut(s) 10, 309
BspCNI CTCAG 2 cut(s) 523, 536
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 1 cut(s) 162
BsrI ACTGG 2 cut(s) 200, 440
BssECI CCNNGG 3 cut(s) 5, 471, 569
BssMI GATC 2 cut(s) 154, 250
BssNI GRCGYC 1 cut(s) 477
BssT1I CCWWGG 2 cut(s) 471, 569
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 477
BstDEI CTNAG 4 cut(s) 161, 502, 523, 531
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 4 cut(s) 111, 517, 541, 547
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 157, 253
BstMAI GTCTC 2 cut(s) 68, 314
BstMBI GATC 2 cut(s) 154, 250
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 5
BstZI CGGCCG 1 cut(s) 8
BsuRI GGCC 2 cut(s) 10, 309
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 4 cut(s) 111, 517, 541, 547
BtsIMutI CAGTG 3 cut(s) 119, 207, 433
BveI ACCTGC 1 cut(s) 275
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 299
CviAII CATG 2 cut(s) 207, 570
CviJI RGCY 2 cut(s) 10, 309
CviKI_1 RGCY 2 cut(s) 10, 309
DdeI CTNAG 4 cut(s) 161, 502, 523, 531
DpnI GATC 2 cut(s) 156, 252
DpnII GATC 2 cut(s) 154, 250
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 2 cut(s) 8, 307
EagI CGGCCG 1 cut(s) 8
Eam1104I CTCTTC 1 cut(s) 398
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 398
EclXI CGGCCG 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 471, 569
Eco52I CGGCCG 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 422
EcoT14I CCWWGG 2 cut(s) 471, 569
EcoT22I ATGCAT 1 cut(s) 547
ErhI CCWWGG 2 cut(s) 471, 569
FaeI CATG 2 cut(s) 210, 573
FatI CATG 2 cut(s) 206, 569
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 1 cut(s) 250
FokI GGATG 4 cut(s) 118, 524, 548, 554
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 298
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 2 cut(s) 10, 309
HapII CCGG 1 cut(s) 7
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 2 cut(s) 210, 573
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 3 cut(s) 58, 146, 527
HpaII CCGG 1 cut(s) 7
HphI GGTGA 3 cut(s) 97, 245, 332
Hpy188I TCNGA 3 cut(s) 63, 526, 532
Hpy188III TCNNGA 2 cut(s) 71, 290
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 2 cut(s) 34, 207
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 4 cut(s) 161, 502, 523, 531
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 2 cut(s) 210, 573
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 154, 250
LmnI GCTCC 1 cut(s) 571
LpnPI CCDG 8 cut(s) 20, 56, 114, 213, 247, 280, 303, 421
LweI GCATC 1 cut(s) 532
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 4 cut(s) 116, 262, 338, 431
MalI GATC 2 cut(s) 156, 252
MboI GATC 2 cut(s) 154, 250
MboII GAAGA 2 cut(s) 415, 467
MhlI GDGCHC 1 cut(s) 568
MluCI AATT 2 cut(s) 218, 303
MlyI GAGTC 1 cut(s) 536
Mph1103I ATGCAT 1 cut(s) 547
MseI TTAA 3 cut(s) 80, 258, 366
MslI CAYNNNNRTG 1 cut(s) 258
MspI CCGG 1 cut(s) 7
MspR9I CCNGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 230
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 569
NdeII GATC 2 cut(s) 154, 250
NlaIII CATG 2 cut(s) 210, 573
NmuCI GTSAC 2 cut(s) 338, 431
NsiI ATGCAT 1 cut(s) 547
PfeI GAWTC 2 cut(s) 58, 146
PflFI GACNNNGTC 1 cut(s) 479
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 530
PsyI GACNNNGTC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 258
SaqAI TTAA 3 cut(s) 80, 258, 366
Sau3AI GATC 2 cut(s) 154, 250
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 1 cut(s) 568
SetI ASST 9 cut(s) 45, 87, 123, 133, 258, 269, 357, 410, 480
SfaNI GCATC 1 cut(s) 532
SmiMI CAYNNNNRTG 1 cut(s) 258
Sse9I AATT 2 cut(s) 218, 303
SsiI CCGC 1 cut(s) 224
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 2 cut(s) 471, 569
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 1 cut(s) 480
TaqI TCGA 1 cut(s) 550
TasI AATT 2 cut(s) 218, 303
TfiI GAWTC 2 cut(s) 58, 146
Tru1I TTAA 3 cut(s) 80, 258, 366
Tru9I TTAA 3 cut(s) 80, 258, 366
TscAI CASTG 3 cut(s) 119, 207, 440
TseFI GTSAC 2 cut(s) 338, 431
Tsp45I GTSAC 2 cut(s) 338, 431
TspRI CASTG 3 cut(s) 119, 207, 440
Tth111I GACNNNGTC 1 cut(s) 479
XspI CTAG 1 cut(s) 465
ZraI GACGTC 1 cut(s) 478
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.