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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 103 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 141, 173 |
| AluBI | AGCT | 3 cut(s) | 118, 188, 247 |
| AluI | AGCT | 3 cut(s) | 118, 188, 247 |
| Alw21I | GWGCWC | 1 cut(s) | 249 |
| AoxI | GGCC | 4 cut(s) | 70, 103, 197, 217 |
| AspS9I | GGNCC | 2 cut(s) | 71, 218 |
| AsuHPI | GGTGA | 1 cut(s) | 125 |
| BalI | TGGCCA | 1 cut(s) | 105 |
| BanII | GRGCYC | 2 cut(s) | 237, 249 |
| Bbv12I | GWGCWC | 1 cut(s) | 249 |
| BfaI | CTAG | 2 cut(s) | 38, 189 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BmgT120I | GGNCC | 2 cut(s) | 71, 218 |
| BmiI | GGNNCC | 2 cut(s) | 139, 220 |
| BmsI | GCATC | 1 cut(s) | 102 |
| BpuEI | CTTGAG | 1 cut(s) | 146 |
| BsaBI | GATNNNNATC | 1 cut(s) | 9 |
| BsaJI | CCNNGG | 2 cut(s) | 32, 200 |
| Bse1I | ACTGG | 2 cut(s) | 200, 220 |
| Bse8I | GATNNNNATC | 1 cut(s) | 9 |
| BseDI | CCNNGG | 2 cut(s) | 32, 200 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BseJI | GATNNNNATC | 1 cut(s) | 9 |
| BseNI | ACTGG | 2 cut(s) | 200, 220 |
| BseRI | GAGGAG | 1 cut(s) | 257 |
| BshFI | GGCC | 4 cut(s) | 72, 105, 199, 219 |
| BsiHKAI | GWGCWC | 1 cut(s) | 249 |
| BsmI | GAATGC | 1 cut(s) | 212 |
| BsnI | GGCC | 4 cut(s) | 72, 105, 199, 219 |
| Bsp1286I | GDGCHC | 2 cut(s) | 237, 249 |
| Bsp19I | CCATGG | 1 cut(s) | 32 |
| BspANI | GGCC | 4 cut(s) | 72, 105, 199, 219 |
| BspLI | GGNNCC | 2 cut(s) | 139, 220 |
| BsrI | ACTGG | 2 cut(s) | 200, 220 |
| BssECI | CCNNGG | 2 cut(s) | 32, 200 |
| BssT1I | CCWWGG | 2 cut(s) | 32, 200 |
| BstDSI | CCRYGG | 1 cut(s) | 32 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 216 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 229 |
| BsuRI | GGCC | 4 cut(s) | 72, 105, 199, 219 |
| BtgI | CCRYGG | 1 cut(s) | 32 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| BtsIMutI | CAGTG | 2 cut(s) | 193, 213 |
| Cfr13I | GGNCC | 2 cut(s) | 71, 218 |
| CviAII | CATG | 1 cut(s) | 33 |
| EaeI | YGGCCR | 1 cut(s) | 103 |
| Ecl136II | GAGCTC | 1 cut(s) | 247 |
| Eco130I | CCWWGG | 2 cut(s) | 32, 200 |
| Eco24I | GRGCYC | 2 cut(s) | 237, 249 |
| Eco53kI | GAGCTC | 1 cut(s) | 247 |
| EcoICRI | GAGCTC | 1 cut(s) | 247 |
| EcoT14I | CCWWGG | 2 cut(s) | 32, 200 |
| EcoT38I | GRGCYC | 2 cut(s) | 237, 249 |
| ErhI | CCWWGG | 2 cut(s) | 32, 200 |
| FaeI | CATG | 1 cut(s) | 36 |
| FaiI | YATR | 9 cut(s) | 18, 34, 81, 86, 102, 153, 173, 175, 263 |
| FatI | CATG | 1 cut(s) | 32 |
| FokI | GGATG | 1 cut(s) | 17 |
| FriOI | GRGCYC | 2 cut(s) | 237, 249 |
| FspBI | CTAG | 2 cut(s) | 38, 189 |
| HaeIII | GGCC | 4 cut(s) | 72, 105, 199, 219 |
| Hin1II | CATG | 1 cut(s) | 36 |
| HinfI | GANTC | 3 cut(s) | 10, 20, 226 |
| HphI | GGTGA | 1 cut(s) | 125 |
| Hpy188I | TCNGA | 1 cut(s) | 25 |
| Hpy188III | TCNNGA | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 1 cut(s) | 93 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 216 |
| Hsp92II | CATG | 1 cut(s) | 36 |
| LmnI | GCTCC | 1 cut(s) | 244 |
| LpnPI | CCDG | 6 cut(s) | 66, 119, 181, 181, 201, 215 |
| LweI | GCATC | 1 cut(s) | 102 |
| MaeI | CTAG | 2 cut(s) | 38, 189 |
| MaeIII | GTNAC | 1 cut(s) | 191 |
| MhlI | GDGCHC | 2 cut(s) | 237, 249 |
| MlsI | TGGCCA | 1 cut(s) | 105 |
| MluCI | AATT | 1 cut(s) | 44 |
| MluNI | TGGCCA | 1 cut(s) | 105 |
| MnlI | CCTC | 5 cut(s) | 128, 140, 221, 235, 247 |
| Mox20I | TGGCCA | 1 cut(s) | 105 |
| MscI | TGGCCA | 1 cut(s) | 105 |
| Msp20I | TGGCCA | 1 cut(s) | 105 |
| Mva1269I | GAATGC | 1 cut(s) | 212 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 216 |
| NcoI | CCATGG | 1 cut(s) | 32 |
| NlaIII | CATG | 1 cut(s) | 36 |
| NlaIV | GGNNCC | 2 cut(s) | 139, 220 |
| NmuCI | GTSAC | 1 cut(s) | 191 |
| PctI | GAATGC | 1 cut(s) | 212 |
| PfeI | GAWTC | 3 cut(s) | 10, 20, 226 |
| Psp124BI | GAGCTC | 1 cut(s) | 249 |
| PspN4I | GGNNCC | 2 cut(s) | 139, 220 |
| PspPI | GGNCC | 2 cut(s) | 71, 218 |
| SacI | GAGCTC | 1 cut(s) | 249 |
| Sau96I | GGNCC | 2 cut(s) | 71, 218 |
| SduI | GDGCHC | 2 cut(s) | 237, 249 |
| SetI | ASST | 4 cut(s) | 120, 139, 190, 249 |
| SfaNI | GCATC | 1 cut(s) | 102 |
| SmlI | CTYRAG | 1 cut(s) | 161 |
| SmoI | CTYRAG | 1 cut(s) | 161 |
| Sse9I | AATT | 1 cut(s) | 44 |
| SspMI | CTAG | 2 cut(s) | 38, 189 |
| SstI | GAGCTC | 1 cut(s) | 249 |
| StyI | CCWWGG | 2 cut(s) | 32, 200 |
| TaqI | TCGA | 1 cut(s) | 252 |
| TasI | AATT | 1 cut(s) | 44 |
| TfiI | GAWTC | 3 cut(s) | 10, 20, 226 |
| TscAI | CASTG | 2 cut(s) | 200, 220 |
| TseFI | GTSAC | 1 cut(s) | 191 |
| Tsp45I | GTSAC | 1 cut(s) | 191 |
| TspGWI | ACGGA | 1 cut(s) | 254 |
| TspRI | CASTG | 2 cut(s) | 200, 220 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 65 |
| XspI | CTAG | 2 cut(s) | 38, 189 |