RLG00000002622

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000653
Physical Location & Seq
Reverse (-)
37135410 .. 37138639
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002622

Sequence Viewer

Length: 1755 bp
ATGGTTGTGATCGAAATTTCCGACGAGAAATTACTTTTTACGGACGAGGAATATGGAAGAAGGCGCTTGATTATACCAGAGGACGAAGCCGCGGAGTTCGAGGCTCAGGCGAGAAGGAGATGGAAGAAGGAAAATAGAAAGCTTCACATACCGAATCTGAACAACAGAGCCGAAGTCGCGGAGCTTAGGAAAAGGCTTCAAAGGAAGACAAGCTCTGACCCTGAAAAAGAAGAAGATGGAGAAGTTAGGGATTTTGTATATGGCTGTGGTGTTTGTGGTAACATGGATGAGAATCACGATGATCGGGAGTGCCCATGGCTAGATAAACTCCCACCACACAAAACTGTTGTTGGCCCTTACTATGACATAGTTTGCATCGGCTGTGGCCAGATTGGTGAGCTGTGCTGTTCCAAGAGAGGTTCCAAGCGAGGATATGCTGTTCTCAAGTCCTGTATATTTTGTCAGACAGCTGGTCACTGGCCTTGGGAATGCCTCACTGGCCCCAAGATTCCGGGGCTCACAGAGGAACTCGTTGAGGAGGTCATATCCTTTGAACCACCCTGCAAGACTGTACTGTTTAGCCAACAATTACACTTTAGGCAAGTATTGCTGGAGAAGGACGACGATGACGATGATGAGGACGACGATGACGATGATGAGGACGAGGATAATGTGTTGAAGGAAGTCATGGAAGAGGATGATGAGGATGGGTTGAAGAAATTCGAGGAAGAGGATGATGATAATGTGTCAGAGGAAGTTACTAAAGAGGATGAGGATGTAATGAAGGAAGTCAAGGAAGAGGAAGGAGGGGATCAAGCTATGGTAGTGATTGAAAATATACCAGAGCACAAAACCGACGAGAAATTATATTTTACGGATGAGGAATATGCAAGAAGGCGCTTGATTATACCAGAGGACGAAGCTGCCGAGTTCGAGGCTCAGGCGAGAAGGAGATGGAAGAAGGAAAATAGAAAGCTCCACATACCGAATCTGAACAACAGAGCCGAAGTTGCGGAGCTTAGGAAAAGGCTTCAAAGGAAGAGAAGCTCTGACCCTGAAAAAGAAGAAGATGGAGAAGTTCGAGATTATGAATATGGCTGTGGTGTTTGTGGTAACATGGATGAGAATCACGATGATCGGGAGTGCCCATGGCTAGATAAACTCCCACCACACAAAACTGTTGTCGGCCCTTACTATGACATAGTTTGCGTCTGCTGTGGCCAGATTGGTGAGCTGTGCTGTTCCAAGAGAGGTTCCAAGCGAGGATATGCTGTTCTCAAGTCCTGTATATTTTGTCAGGCAGCTGGTCACTGGCCTTGGGAATGCCTCACTGGCCCCAAGATTCCAGGGCTCACAGAGGAACTCGTCGAGGTCATATCCCTTGAACCATCCTGCAAGAACATGGAAGAGTATGAGGTTGTGTTTGAGGATGCGACTTACACCAAGGAAGATGGGGCTCAAGGTGGTTATTCTATTCGAGATACTCACCGGCCTTGGGAATGCCCCAAGGACCCGAAGATTCCAGGGCTCTCAGAGATTTTCATCAAGGCTGTCCTATCCTGCAAGAACATGGAAGGGGATGAGGATGAGGAAGATGGGGCTCAAGGTGGAAATGAATCTTCAAAGCAGAGCTGGAGTAAGAAAAAGACTGGTGAGGTATTGTTGAACCCAGGGAATGTTGCCAAGAAGCGTAGAATCGATTTTGAGGTAAGCCGCTATCTGATTGTCTTTACATGTAATGGATTGTTTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

585

Amino Acids

67.13

Weight (kDa)

4.68

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 92, 179
AciI CCGC 5 cut(s) 90, 92, 179, 1013, 1714
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 2 cut(s) 385, 1219
AcsI RAATTY 2 cut(s) 15, 719
AfaI GTAC 1 cut(s) 573
AfiI CCNNNNNNNGG 1 cut(s) 1495
AflIII ACRYGT 1 cut(s) 1733
AgsI TTSAA 9 cut(s) 200, 554, 679, 715, 833, 1034, 1385, 1623, 1666
AhdI GACNNNNNGTC 1 cut(s) 471
AjnI CCWGG 3 cut(s) 1345, 1522, 1669
AloI GAACNNNNNNTCC 4 cut(s) 423, 455, 1257, 1289
Alw21I GWGCWC 1 cut(s) 849
AlwI GGATC 1 cut(s) 819
AoxI GGCC 9 cut(s) 352, 385, 479, 499, 1186, 1219, 1313, 1333, 1490
ApeKI GCWGC 2 cut(s) 923, 1301
ApoI RAATTY 2 cut(s) 15, 719
ArsI GACNNNNNNTTYG 2 cut(s) 1167, 1199
Asp700I GAANNNNTTC 1 cut(s) 719
AspLEI GCGC 2 cut(s) 66, 900
AspS9I GGNCC 5 cut(s) 353, 500, 1187, 1334, 1510
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 407, 1241, 1478, 1664
AvaII GGWCC 1 cut(s) 1510
BaeGI GKGCMC 2 cut(s) 314, 1148
BalI TGGCCA 2 cut(s) 387, 1221
BanII GRGCYC 5 cut(s) 519, 1353, 1459, 1530, 1603
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 2 cut(s) 910, 1313
BccI CCATC 8 cut(s) 114, 230, 701, 948, 1064, 1396, 1445, 1589
BciT130I CCWGG 3 cut(s) 1347, 1524, 1671
BcnI CCSGG 1 cut(s) 513
BfaI CTAG 2 cut(s) 320, 1154
BfoI RGCGCY 2 cut(s) 67, 901
BglI GCCNNNNNGGC 2 cut(s) 498, 1332
BisI GCNGC 4 cut(s) 90, 924, 1302, 1714
BlsI GCNGC 4 cut(s) 91, 925, 1303, 1715
Bme1390I CCNGG 4 cut(s) 513, 1347, 1524, 1671
Bme18I GGWCC 1 cut(s) 1510
BmeRI GACNNNNNGTC 1 cut(s) 471
BmgT120I GGNCC 5 cut(s) 353, 500, 1187, 1334, 1510
BmiI GGNNCC 5 cut(s) 421, 502, 1255, 1336, 1512
BmrFI CCNGG 4 cut(s) 513, 1347, 1524, 1671
BmsI GCATC 2 cut(s) 384, 1420
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 2 cut(s) 632, 1654
Bpu10I CCTNAGC 4 cut(s) 105, 185, 939, 1019
BpuEI CTTGAG 4 cut(s) 428, 1262, 1443, 1587
BpuMI CCSGG 1 cut(s) 513
Bsa29I ATCGAT 1 cut(s) 1698
BsaBI GATNNNNATC 3 cut(s) 291, 1125, 1541
Bsc4I CCNNNNNNNGG 1 cut(s) 1495
Bse118I RCCGGY 1 cut(s) 1488
Bse1I ACTGG 5 cut(s) 482, 502, 1316, 1336, 1654
Bse8I GATNNNNATC 3 cut(s) 291, 1125, 1541
BseBI CCWGG 3 cut(s) 1347, 1524, 1671
BseCI ATCGAT 1 cut(s) 1698
BseJI GATNNNNATC 3 cut(s) 291, 1125, 1541
BseLI CCNNNNNNNGG 1 cut(s) 1495
BseMII CTCAG 3 cut(s) 119, 953, 1545
BseNI ACTGG 5 cut(s) 482, 502, 1316, 1336, 1654
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 2 cut(s) 314, 1148
BseXI GCAGC 2 cut(s) 910, 1313
Bsh1236I CGCG 2 cut(s) 92, 179
BshFI GGCC 9 cut(s) 354, 387, 481, 501, 1188, 1221, 1315, 1335, 1492
BshVI ATCGAT 1 cut(s) 1698
BsiHKAI GWGCWC 1 cut(s) 849
BsiSI CCGG 2 cut(s) 512, 1489
BslI CCNNNNNNNGG 1 cut(s) 1495
BsmI GAATGC 3 cut(s) 494, 1328, 1505
BsnI GGCC 9 cut(s) 354, 387, 481, 501, 1188, 1221, 1315, 1335, 1492
Bsp1286I GDGCHC 8 cut(s) 314, 519, 849, 1148, 1353, 1459, 1530, 1603
Bsp143I GATC 4 cut(s) 9, 301, 811, 1135
Bsp19I CCATGG 2 cut(s) 314, 1148
BspACI CCGC 5 cut(s) 90, 92, 179, 1013, 1714
BspANI GGCC 9 cut(s) 354, 387, 481, 501, 1188, 1221, 1315, 1335, 1492
BspCNI CTCAG 3 cut(s) 118, 952, 1544
BspDI ATCGAT 1 cut(s) 1698
BspFNI CGCG 2 cut(s) 92, 179
BspLI GGNNCC 5 cut(s) 421, 502, 1255, 1336, 1512
BspPI GGATC 1 cut(s) 819
BsrFI RCCGGY 1 cut(s) 1488
BsrI ACTGG 5 cut(s) 482, 502, 1316, 1336, 1654
BssAI RCCGGY 1 cut(s) 1488
BssMI GATC 4 cut(s) 9, 301, 811, 1135
BssT1I CCWWGG 7 cut(s) 314, 482, 1148, 1316, 1443, 1493, 1506
Bst2UI CCWGG 3 cut(s) 1347, 1524, 1671
Bst4CI ACNGT 4 cut(s) 346, 571, 576, 1180
Bst6I CTCTTC 5 cut(s) 687, 723, 792, 1034, 1401
BstAPI GCANNNNNTGC 1 cut(s) 607
BstDEI CTNAG 5 cut(s) 105, 185, 939, 1019, 1531
BstDSI CCRYGG 3 cut(s) 90, 314, 1148
BstFNI CGCG 2 cut(s) 92, 179
BstH2I RGCGCY 2 cut(s) 67, 901
BstHHI GCGC 2 cut(s) 66, 900
BstKTI GATC 4 cut(s) 12, 304, 814, 1138
BstMBI GATC 4 cut(s) 9, 301, 811, 1135
BstMWI GCNNNNNNNGC 5 cut(s) 176, 498, 607, 1010, 1332
BstNI CCWGG 3 cut(s) 1347, 1524, 1671
BstNSI RCATGY 1 cut(s) 1737
BstSCI CCNGG 4 cut(s) 511, 1345, 1522, 1669
BstSLI GKGCMC 2 cut(s) 314, 1148
BstUI CGCG 2 cut(s) 92, 179
BstV1I GCAGC 2 cut(s) 910, 1313
BstV2I GAAGAC 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 1698
BsuRI GGCC 9 cut(s) 354, 387, 481, 501, 1188, 1221, 1315, 1335, 1492
BsuTUI ATCGAT 1 cut(s) 1698
BtgI CCRYGG 3 cut(s) 90, 314, 1148
BtsIMutI CAGTG 4 cut(s) 475, 495, 1309, 1329
CfoI GCGC 2 cut(s) 66, 900
Cfr10I RCCGGY 1 cut(s) 1488
Cfr13I GGNCC 5 cut(s) 353, 500, 1187, 1334, 1510
Cfr42I CCGCGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 1698
CseI GACGC 1 cut(s) 1198
Csp6I GTAC 1 cut(s) 572
CviAII CATG 8 cut(s) 283, 315, 688, 1117, 1149, 1402, 1570, 1734
CviQI GTAC 1 cut(s) 572
DdeI CTNAG 5 cut(s) 105, 185, 939, 1019, 1531
DpnI GATC 4 cut(s) 11, 303, 813, 1137
DpnII GATC 4 cut(s) 9, 301, 811, 1135
DriI GACNNNNNGTC 1 cut(s) 471
EaeI YGGCCR 2 cut(s) 385, 1219
Eam1104I CTCTTC 5 cut(s) 687, 723, 792, 1034, 1401
Eam1105I GACNNNNNGTC 1 cut(s) 471
EarI CTCTTC 5 cut(s) 687, 723, 792, 1034, 1401
Eco130I CCWWGG 7 cut(s) 314, 482, 1148, 1316, 1443, 1493, 1506
Eco24I GRGCYC 5 cut(s) 519, 1353, 1459, 1530, 1603
Eco47I GGWCC 1 cut(s) 1510
EcoO109I RGGNCCY 1 cut(s) 1510
EcoRII CCWGG 3 cut(s) 1345, 1522, 1669
EcoT14I CCWWGG 7 cut(s) 314, 482, 1148, 1316, 1443, 1493, 1506
EcoT38I GRGCYC 5 cut(s) 519, 1353, 1459, 1530, 1603
ErhI CCWWGG 7 cut(s) 314, 482, 1148, 1316, 1443, 1493, 1506
FaeI CATG 8 cut(s) 286, 318, 691, 1120, 1152, 1405, 1573, 1737
FatI CATG 8 cut(s) 282, 314, 687, 1116, 1148, 1401, 1569, 1733
Fnu4HI GCNGC 4 cut(s) 90, 924, 1302, 1714
FriOI GRGCYC 5 cut(s) 519, 1353, 1459, 1530, 1603
Fsp4HI GCNGC 4 cut(s) 90, 924, 1302, 1714
FspBI CTAG 2 cut(s) 320, 1154
GlaI GCGC 2 cut(s) 65, 899
GluI GCNGC 4 cut(s) 90, 924, 1302, 1714
GsuI CTGGAG 2 cut(s) 632, 1654
HaeII RGCGCY 2 cut(s) 67, 901
HaeIII GGCC 9 cut(s) 354, 387, 481, 501, 1188, 1221, 1315, 1335, 1492
HapII CCGG 2 cut(s) 512, 1489
HgaI GACGC 1 cut(s) 1198
HhaI GCGC 2 cut(s) 66, 900
Hin1II CATG 8 cut(s) 286, 318, 691, 1120, 1152, 1405, 1573, 1737
Hin6I GCGC 2 cut(s) 64, 898
HinP1I GCGC 2 cut(s) 64, 898
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 9 cut(s) 154, 292, 508, 988, 1126, 1342, 1519, 1616, 1695
HpaII CCGG 2 cut(s) 512, 1489
HphI GGTGA 4 cut(s) 407, 1241, 1478, 1664
Hpy188I TCNGA 9 cut(s) 22, 159, 217, 465, 751, 993, 1051, 1534, 1722
Hpy188III TCNNGA 6 cut(s) 296, 305, 1082, 1130, 1139, 1478
Hpy99I CGWCG 5 cut(s) 26, 626, 647, 860, 1370
HpyCH4III ACNGT 4 cut(s) 346, 571, 576, 1180
HpyCH4V TGCA 5 cut(s) 375, 564, 890, 1395, 1563
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 498, 607, 1010, 1332
HpyF3I CTNAG 5 cut(s) 105, 185, 939, 1019, 1531
Hsp92II CATG 8 cut(s) 286, 318, 691, 1120, 1152, 1405, 1573, 1737
HspAI GCGC 2 cut(s) 64, 898
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 4 cut(s) 9, 301, 811, 1135
LmnI GCTCC 3 cut(s) 181, 981, 1015
Lsp1109I GCAGC 2 cut(s) 910, 1313
LweI GCATC 2 cut(s) 384, 1420
MaeI CTAG 2 cut(s) 320, 1154
MaeIII GTNAC 5 cut(s) 278, 473, 757, 1112, 1307
MalI GATC 4 cut(s) 11, 303, 813, 1137
MboI GATC 4 cut(s) 9, 301, 811, 1135
MhlI GDGCHC 8 cut(s) 314, 519, 849, 1148, 1353, 1459, 1530, 1603
MlsI TGGCCA 2 cut(s) 387, 1221
MluCI AATT 5 cut(s) 15, 29, 587, 719, 863
MluNI TGGCCA 2 cut(s) 387, 1221
MmeI TCCRAC 1 cut(s) 45
Mox20I TGGCCA 2 cut(s) 387, 1221
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 2 cut(s) 387, 1221
Msp20I TGGCCA 2 cut(s) 387, 1221
MspA1I CMGCKG 3 cut(s) 92, 470, 1304
MspI CCGG 2 cut(s) 512, 1489
MspR9I CCNGG 4 cut(s) 513, 1347, 1524, 1671
Mva1269I GAATGC 3 cut(s) 494, 1328, 1505
MvaI CCWGG 3 cut(s) 1347, 1524, 1671
MvnI CGCG 2 cut(s) 92, 179
MwoI GCNNNNNNNGC 5 cut(s) 176, 498, 607, 1010, 1332
NciI CCSGG 1 cut(s) 513
NcoI CCATGG 2 cut(s) 314, 1148
NdeII GATC 4 cut(s) 9, 301, 811, 1135
NlaIII CATG 8 cut(s) 286, 318, 691, 1120, 1152, 1405, 1573, 1737
NlaIV GGNNCC 5 cut(s) 421, 502, 1255, 1336, 1512
NmeAIII GCCGAG 1 cut(s) 952
NmuCI GTSAC 2 cut(s) 473, 1307
NspI RCATGY 1 cut(s) 1737
PasI CCCWGGG 1 cut(s) 1670
PciI ACATGT 1 cut(s) 1733
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 627, 648, 924
PctI GAATGC 3 cut(s) 494, 1328, 1505
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 9 cut(s) 154, 292, 508, 988, 1126, 1342, 1519, 1616, 1695
PkrI GCNGC 4 cut(s) 91, 925, 1303, 1715
PpuMI RGGWCCY 1 cut(s) 1510
PscI ACATGT 1 cut(s) 1733
Psp5II RGGWCCY 1 cut(s) 1510
Psp6I CCWGG 3 cut(s) 1345, 1522, 1669
PspGI CCWGG 3 cut(s) 1345, 1522, 1669
PspN4I GGNNCC 5 cut(s) 421, 502, 1255, 1336, 1512
PspPI GGNCC 5 cut(s) 353, 500, 1187, 1334, 1510
PspPPI RGGWCCY 1 cut(s) 1510
PvuII CAGCTG 2 cut(s) 470, 1304
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SacII CCGCGG 1 cut(s) 93
SatI GCNGC 4 cut(s) 90, 924, 1302, 1714
Sau3AI GATC 4 cut(s) 9, 301, 811, 1135
Sau96I GGNCC 5 cut(s) 353, 500, 1187, 1334, 1510
ScrFI CCNGG 4 cut(s) 513, 1347, 1524, 1671
SduI GDGCHC 8 cut(s) 314, 519, 849, 1148, 1353, 1459, 1530, 1603
SfaNI GCATC 2 cut(s) 384, 1420
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 1 cut(s) 1510
SmlI CTYRAG 4 cut(s) 443, 1277, 1458, 1602
SmoI CTYRAG 4 cut(s) 443, 1277, 1458, 1602
Sse9I AATT 5 cut(s) 15, 29, 587, 719, 863
SsiI CCGC 5 cut(s) 90, 92, 179, 1013, 1714
SspMI CTAG 2 cut(s) 320, 1154
StyD4I CCNGG 4 cut(s) 511, 1345, 1522, 1669
StyI CCWWGG 7 cut(s) 314, 482, 1148, 1316, 1443, 1493, 1506
TaaI ACNGT 4 cut(s) 346, 571, 576, 1180
TaqI TCGA 8 cut(s) 12, 99, 723, 933, 1081, 1368, 1477, 1698
TasI AATT 5 cut(s) 15, 29, 587, 719, 863
TatI WGTACW 1 cut(s) 571
TauI GCSGC 2 cut(s) 92, 1716
TfiI GAWTC 9 cut(s) 154, 292, 508, 988, 1126, 1342, 1519, 1616, 1695
TscAI CASTG 4 cut(s) 482, 502, 1316, 1336
TseFI GTSAC 2 cut(s) 473, 1307
TseI GCWGC 2 cut(s) 923, 1301
Tsp45I GTSAC 2 cut(s) 473, 1307
TspDTI ATGAA 4 cut(s) 797, 1104, 1531, 1629
TspGWI ACGGA 2 cut(s) 56, 890
TspRI CASTG 4 cut(s) 482, 502, 1316, 1336
VpaK11BI GGWCC 1 cut(s) 1510
XapI RAATTY 2 cut(s) 15, 719
XceI RCATGY 1 cut(s) 1737
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 2 cut(s) 320, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.