Rroxscaffold_4G00280340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000653
Physical Location & Seq
Forward (+)
2904891 .. 2905829
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280340.1

Sequence Viewer

Length: 516 bp
ATGGGATTAAGAGATCCAGATCAACTCGAGAAGAAGCTGCCGGAAAATATCAAATTCCCTGAAGCCAAGTGGATGGATCTGGATCTGGCCGGCGAGAACATGACTCATGCCAACAAGAACAGGACACATTGTAGCAAGGGGGAAGAATCAAACAATGCTGAAGACTATTACAGTCTGGGCACATTTCTGCGTGACCTCACAGCTATCTCCAAAAATGAAGAAAACAGTGAAGATGGATATAGTGAAGAAGAAGGAGAGGGTTGTCCCATTTGTTTCAATCTGGATCACAATAACAATAACTGCCCATGGCTAGTTAATGTTCCACCAGGTAAAACAGTTGGGCGTCTCTATGACAGAGAATGCAAGGGTTGTGGTAAGTTGGGACCTGCATGCTGTTATGAGGGAGGACGTGCTGTTCTCAGAAGGTGTGGTCATTGTTTTGATTTTGGTCACTGGGGAAACGACTGCCCCAACTATAAGTATATGCCCCTTTCGGCCTTCCCAGATTATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

19.31

Weight (kDa)

5.03

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 394
AclWI GGATC 4 cut(s) 8, 84, 90, 291
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 2 cut(s) 81, 180
AcyI GRCGYC 1 cut(s) 343
AgsI TTSAA 1 cut(s) 277
AjiI CACGTC 1 cut(s) 410
AjnI CCWGG 1 cut(s) 325
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 2 cut(s) 37, 203
AluI AGCT 2 cut(s) 37, 203
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 4 cut(s) 8, 84, 90, 291
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 87, 495
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 24
BccI CCATC 2 cut(s) 67, 227
BciT130I CCWGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 383
BmeT110I CYCGRG 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 463
BpiI GAAGAC 1 cut(s) 168
BsaBI GATNNNNATC 2 cut(s) 18, 81
BsaHI GRCGYC 1 cut(s) 343
BsaJI CCNNGG 1 cut(s) 305
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 458
Bse8I GATNNNNATC 2 cut(s) 18, 81
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 1 cut(s) 78
BseJI GATNNNNATC 2 cut(s) 18, 81
BseMII CTCAG 1 cut(s) 433
BseNI ACTGG 1 cut(s) 458
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 1 cut(s) 24
BshFI GGCC 2 cut(s) 89, 497
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 41, 90
BslFI GGGAC 2 cut(s) 249, 396
BsmAI GTCTC 1 cut(s) 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 249, 396
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 2 cut(s) 89, 497
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 5 cut(s) 13, 19, 76, 82, 283
Bsp19I CCATGG 1 cut(s) 305
BspANI GGCC 2 cut(s) 89, 497
BspCNI CTCAG 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 4 cut(s) 8, 84, 90, 291
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 5 cut(s) 13, 19, 76, 82, 283
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 3 cut(s) 173, 227, 337
BstACI GRCGYC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 91, 391
BstDEI CTNAG 1 cut(s) 419
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 5 cut(s) 16, 22, 79, 85, 286
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 5 cut(s) 13, 19, 76, 82, 283
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 325
BstSLI GKGCMC 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 3 cut(s) 13, 76, 82
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 3 cut(s) 13, 76, 82
BsuRI GGCC 2 cut(s) 89, 497
BtgI CCRYGG 1 cut(s) 305
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 232, 451
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 91, 391
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 383
CseI GACGC 1 cut(s) 332
CsiI ACCWGGT 1 cut(s) 325
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 5 cut(s) 100, 107, 306, 390, 513
CviJI RGCY 6 cut(s) 37, 65, 89, 203, 310, 497
CviKI_1 RGCY 6 cut(s) 37, 65, 89, 203, 310, 497
DdeI CTNAG 1 cut(s) 419
DpnI GATC 5 cut(s) 15, 21, 78, 84, 285
DpnII GATC 5 cut(s) 13, 19, 76, 82, 283
EaeI YGGCCR 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 2 cut(s) 81, 180
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 5 cut(s) 103, 110, 309, 393, 516
FaqI GGGAC 2 cut(s) 249, 396
FatI CATG 5 cut(s) 99, 106, 305, 389, 512
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 311
GluI GCNGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 89, 497
HapII CCGG 2 cut(s) 41, 90
HgaI GACGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 5 cut(s) 103, 110, 309, 393, 516
HinfI GANTC 2 cut(s) 103, 146
HpaII CCGG 2 cut(s) 41, 90
Hpy188I TCNGA 1 cut(s) 422
Hpy188III TCNNGA 4 cut(s) 17, 28, 80, 281
HpyAV CCTTC 3 cut(s) 245, 417, 508
HpyCH4III ACNGT 3 cut(s) 173, 227, 337
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 2 cut(s) 363, 389
HpyF3I CTNAG 1 cut(s) 419
HpySE526I ACGT 1 cut(s) 409
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 5 cut(s) 103, 110, 309, 393, 516
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Kzo9I GATC 5 cut(s) 13, 19, 76, 82, 283
Lsp1109I GCAGC 1 cut(s) 24
MabI ACCWGGT 1 cut(s) 325
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 2 cut(s) 191, 449
MalI GATC 5 cut(s) 15, 21, 78, 84, 285
MboI GATC 5 cut(s) 13, 19, 76, 82, 283
MboII GAAGA 7 cut(s) 43, 155, 173, 230, 242, 257, 260
MflI RGATCY 3 cut(s) 13, 76, 82
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 1 cut(s) 53
MlyI GAGTC 1 cut(s) 97
MnlI CCTC 4 cut(s) 206, 250, 394, 398
MroNI GCCGGC 1 cut(s) 89
MseI TTAA 2 cut(s) 8, 315
MspI CCGG 2 cut(s) 41, 90
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 1 cut(s) 327
NaeI GCCGGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 305
NdeII GATC 5 cut(s) 13, 19, 76, 82, 283
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 103, 110, 309, 393, 516
NlaIV GGNNCC 1 cut(s) 384
NmuCI GTSAC 2 cut(s) 191, 449
NspI RCATGY 1 cut(s) 393
PaeI GCATGC 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 26
PctI GAATGC 1 cut(s) 365
PdiI GCCGGC 1 cut(s) 91
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 1 cut(s) 383
PspPPI RGGWCCY 1 cut(s) 383
PsuI RGATCY 3 cut(s) 13, 76, 82
SaqAI TTAA 2 cut(s) 8, 315
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 5 cut(s) 13, 19, 76, 82, 283
Sau96I GGNCC 1 cut(s) 383
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 182
SetI ASST 7 cut(s) 39, 198, 205, 331, 388, 412, 428
SexAI ACCWGGT 1 cut(s) 325
Sfr274I CTCGAG 1 cut(s) 26
SinI GGWCC 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SphI GCATGC 1 cut(s) 393
Sse9I AATT 1 cut(s) 53
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 3 cut(s) 173, 227, 337
TaiI ACGT 1 cut(s) 412
TaqI TCGA 1 cut(s) 27
TasI AATT 1 cut(s) 53
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 2 cut(s) 8, 315
Tru9I TTAA 2 cut(s) 8, 315
TscAI CASTG 2 cut(s) 232, 458
TseFI GTSAC 2 cut(s) 191, 449
TseI GCWGC 1 cut(s) 37
Tsp45I GTSAC 2 cut(s) 191, 449
TspDTI ATGAA 1 cut(s) 231
TspRI CASTG 2 cut(s) 232, 458
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 26
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.