Rh1DG422000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000653
Physical Location & Seq
Reverse (-)
63616373 .. 63616738
366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG422000.1

Sequence Viewer

Length: 366 bp
ATGAAAACAAATGATGCTGGAGAGTCTTATCTCCGTATGGGCGACTATGTGTTTGTGTATGTGAAAGACTCTAAAAAGGAAGAAATCAGAGAAGAAGAAGAAGCAGAAGACGGTTGTCCCATCTGTCTTGGGGACCACTTTAACTTGGAATGCCCATGGCGAGATAATGTTCCACCAGGCCAACAAGTTGGGCGTCTCTATGACATAAAATGCAAGGGTTGTGGTAAGTGGGGACTTGCATGCTGTGCTGAGGGAGGACTTGCTGTTCTCAGGAGGTGTGGTCATTGTTTTGATTTTGGTCACTGGGGGGACGTCTGCCCCGACTATAAGTATATGCCCCTTTCGGCCTTCCCAAATTATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.71

Weight (kDa)

4.99

Isoelectric Point (pI)

56.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
AcyI GRCGYC 2 cut(s) 193, 312
AjnI CCWGG 1 cut(s) 175
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw26I GTCTC 1 cut(s) 200
AoxI GGCC 2 cut(s) 178, 345
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AspS9I GGNCC 1 cut(s) 133
AvaII GGWCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 249
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 200
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 177
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 4
BmuI ACTGGG 1 cut(s) 313
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 249
BsaHI GRCGYC 2 cut(s) 193, 312
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bse1I ACTGG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 155
BseMII CTCAG 2 cut(s) 240, 283
BseNI ACTGG 1 cut(s) 308
BshFI GGCC 2 cut(s) 180, 347
BslFI GGGAC 4 cut(s) 102, 146, 246, 323
BsmAI GTCTC 1 cut(s) 200
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 4 cut(s) 102, 146, 246, 323
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 2 cut(s) 180, 347
Bsp19I CCATGG 1 cut(s) 155
BspANI GGCC 2 cut(s) 180, 347
BspCNI CTCAG 2 cut(s) 241, 282
BspLI GGNNCC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 155
BssNI GRCGYC 2 cut(s) 193, 312
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 113
BstACI GRCGYC 2 cut(s) 193, 312
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 2 cut(s) 249, 269
BstDSI CCRYGG 1 cut(s) 155
BstMAI GTCTC 1 cut(s) 200
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 243
BstPAI GACNNNNGTC 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 188
BsuRI GGCC 2 cut(s) 180, 347
BtgI CCRYGG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 1 cut(s) 182
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 3 cut(s) 156, 240, 363
CviJI RGCY 2 cut(s) 180, 347
CviKI_1 RGCY 2 cut(s) 180, 347
DdeI CTNAG 2 cut(s) 249, 269
Eco130I CCWWGG 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 3 cut(s) 159, 243, 366
FaqI GGGAC 4 cut(s) 102, 146, 246, 323
FatI CATG 3 cut(s) 155, 239, 362
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 180, 347
HgaI GACGC 1 cut(s) 182
Hin1I GRCGYC 2 cut(s) 193, 312
Hin1II CATG 3 cut(s) 159, 243, 366
HinfI GANTC 2 cut(s) 23, 68
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 213, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 2 cut(s) 249, 269
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 2 cut(s) 193, 312
Hsp92II CATG 3 cut(s) 159, 243, 366
LpnPI CCDG 5 cut(s) 3, 162, 189, 256, 289
LweI GCATC 1 cut(s) 4
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 299
MboII GAAGA 5 cut(s) 92, 104, 107, 110, 119
MluCI AATT 1 cut(s) 355
MlyI GAGTC 2 cut(s) 32, 62
MnlI CCTC 3 cut(s) 244, 248, 267
MseI TTAA 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 155
NlaIII CATG 3 cut(s) 159, 243, 366
NlaIV GGNNCC 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 243
PaeI GCATGC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 1 cut(s) 155
PleI GAGTC 2 cut(s) 31, 62
PpsI GAGTC 2 cut(s) 31, 62
PshAI GACNNNNGTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 141
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 2 cut(s) 32, 62
ScrFI CCNGG 1 cut(s) 177
SetI ASST 2 cut(s) 278, 315
SfaNI GCATC 1 cut(s) 4
SinI GGWCC 1 cut(s) 133
SphI GCATGC 1 cut(s) 243
Sse9I AATT 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 113
TaiI ACGT 1 cut(s) 315
TasI AATT 1 cut(s) 355
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 133
XceI RCATGY 1 cut(s) 243
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.