FvH4_6g20312

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000653
Physical Location & Seq
Reverse (-)
13904228 .. 13904723
496 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20312.t1

Sequence Viewer

Length: 360 bp
ATGTCCACTACATACCTTCCAAAGGGTGAGGATGAATTTTCTCAAAATTTGAAGCCGAAAACAAATGACGCTGGAGAGAGTTACTTCCGCATGGGCGACTATGTGTATGTGATAGGCTCTGTAGAGGAAGTTGCACATCCTGAATACGACATTGATGGTTGTCCCATTTGTTTCGGCCTTGATCACGGTAACATGAAGTGCCCATGGCTAGATAAGGTTCCACAAGGTAAAACAGTTGGGCCTATGTATGACATAGAATGCATAGGTTGTGGTAAGCTCAGACATACAGGCTGTTATGAGGGTGAACGTCCTGTTCTCTGGAGGTGTTTTAATTGTTACGGCATTGTGTGTTTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.46

Weight (kDa)

4.92

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 88
AcsI RAATTY 2 cut(s) 35, 46
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 1 cut(s) 52
AluBI AGCT 1 cut(s) 277
AluI AGCT 1 cut(s) 277
AoxI GGCC 2 cut(s) 175, 239
ApoI RAATTY 2 cut(s) 35, 46
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 38, 314
BaeGI GKGCMC 1 cut(s) 203
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 355
BclI TGATCA 1 cut(s) 181
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 219
BpmI CTGGAG 2 cut(s) 93, 340
BsaJI CCNNGG 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 2 cut(s) 37, 136
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 292
BseSI GKGCMC 1 cut(s) 203
BshFI GGCC 2 cut(s) 177, 241
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 2 cut(s) 177, 241
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 1 cut(s) 181
Bsp19I CCATGG 1 cut(s) 203
BspACI CCGC 1 cut(s) 88
BspANI GGCC 2 cut(s) 177, 241
BspCNI CTCAG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 203
BssMI GATC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 188, 235
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 37, 136
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 203
BsuRI GGCC 2 cut(s) 177, 241
BtgI CCRYGG 1 cut(s) 203
BtsCI GGATG 2 cut(s) 37, 136
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 77
CviAII CATG 3 cut(s) 91, 193, 204
CviJI RGCY 7 cut(s) 55, 117, 177, 208, 241, 277, 291
CviKI_1 RGCY 7 cut(s) 55, 117, 177, 208, 241, 277, 291
DdeI CTNAG 1 cut(s) 278
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoT14I CCWWGG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 3 cut(s) 94, 196, 207
FaqI GGGAC 1 cut(s) 147
FatI CATG 3 cut(s) 90, 192, 203
FbaI TGATCA 1 cut(s) 181
FokI GGATG 2 cut(s) 44, 123
FspBI CTAG 1 cut(s) 209
GsuI CTGGAG 2 cut(s) 93, 340
HaeIII GGCC 2 cut(s) 177, 241
HgaI GACGC 1 cut(s) 77
Hin1II CATG 3 cut(s) 94, 196, 207
HphI GGTGA 2 cut(s) 38, 314
Hpy166II GTNNAC 2 cut(s) 6, 305
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 2 cut(s) 140, 319
Hpy8I GTNNAC 2 cut(s) 6, 305
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 2 cut(s) 188, 235
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 2 cut(s) 134, 261
HpyF3I CTNAG 1 cut(s) 278
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 3 cut(s) 94, 196, 207
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 181
LpnPI CCDG 5 cut(s) 57, 153, 273, 304, 324
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 3 cut(s) 80, 188, 335
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 3 cut(s) 35, 46, 331
MnlI CCTC 4 cut(s) 22, 118, 292, 315
Mph1103I ATGCAT 1 cut(s) 263
MseI TTAA 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 263
NcoI CCATGG 1 cut(s) 203
NdeII GATC 1 cut(s) 181
NlaIII CATG 3 cut(s) 94, 196, 207
NlaIV GGNNCC 1 cut(s) 219
NsiI ATGCAT 1 cut(s) 263
PctI GAATGC 1 cut(s) 263
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 203
SetI ASST 7 cut(s) 18, 219, 229, 268, 279, 310, 326
SfcI CTRYAG 1 cut(s) 120
Sse9I AATT 3 cut(s) 35, 46, 331
SsiI CCGC 1 cut(s) 88
SspMI CTAG 1 cut(s) 209
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 188, 235
TaiI ACGT 1 cut(s) 310
TasI AATT 3 cut(s) 35, 46, 331
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TspDTI ATGAA 2 cut(s) 48, 209
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 2 cut(s) 35, 46
XspI CTAG 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.